EP2471366A2 - Synergistic combination of flumetsulam or diclosulam with isothiazolones - Google Patents
Synergistic combination of flumetsulam or diclosulam with isothiazolones Download PDFInfo
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- EP2471366A2 EP2471366A2 EP11184073A EP11184073A EP2471366A2 EP 2471366 A2 EP2471366 A2 EP 2471366A2 EP 11184073 A EP11184073 A EP 11184073A EP 11184073 A EP11184073 A EP 11184073A EP 2471366 A2 EP2471366 A2 EP 2471366A2
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- Prior art keywords
- isothiazolin
- octyl
- ppm
- flumetsulam
- inhibition
- Prior art date
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/80—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/82—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with three ring hetero atoms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N2300/00—Combinations or mixtures of active ingredients covered by classes A01N27/00 - A01N65/48 with other active or formulation relevant ingredients, e.g. specific carrier materials or surfactants, covered by classes A01N25/00 - A01N65/48
Definitions
- This invention relates to combinations of biocides, the combinations having greater activity than would be observed for the individual antimicrobial compounds.
- 5,591,760 discloses a combination of 3-iodo-2-propynyl-butylcarbamate (IPBC) and 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one (DCOIT), but this reference does not suggest any of the combinations claimed herein.
- IPBC 3-iodo-2-propynyl-butylcarbamate
- DCOIT 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one
- the present invention is directed to a synergistic antimicrobial composition
- a synergistic antimicrobial composition comprising: (a) flumetsulam; and (b) an isothiazolone biocide selected from the group consisting of 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one, 2-n-octyl-4-isothiazolin-3-one (OIT) and N-n-butyl-1,2-benzisothiazofin-3-one (BBIT); wherein a weight ratio of flumetsulam to 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one is from 15:1 to 1:15, a weight ratio of flumetsulam to 2-n-octyl-4-isothiazolin-3-one is from 7:1 to 1:5 and a weight ratio of flumetsulam to N-n-butyl-1,2-benzisothiazolin-3
- the present invention is further directed to a synergistic antimicrobial composition
- a synergistic antimicrobial composition comprising: (a) diclosulam; and (b) an isothiazolone biocide selected from the group consisting of 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one and 2-n-octyl-4-isothiazolin-3-one; wherein a weight ratio of diclosulam to 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one is from 7:1 to 1:3 and a weight ratio of diclosulam to 2-n-octyl-4-isothiazolin-3-one is from 7:1 to 1:1.5 or 1:3 to 1:7.
- Fluometsulam is N-(2,6-difluorophenyl)-5-methyl[1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide.
- Diclosulam is N-(2,6-dichlorophenyl)-5-ethoxy-7-fluoro[1,2,4]triazolo[1,5-c]pyrimidine-2-sulfonamide.
- antimicrobial compound refers to a compound capable of inhibiting the growth of or controlling the growth of microorganisms; antimicrobial compounds include bactericides, bacteristats, fungicides, fungistats, algaecides and algistats, depending on the dose level applied, system conditions and the level of microbial control desired.
- temperatures are in degrees centigrade (°C), and references to percentages are by weight (wt%).
- Percentages of antimicrobial compounds in the composition of this invention are based on the total weight of active ingredients in the composition, i.e., the antimicrobial compounds themselves, exclusive of any amounts of solvents, carriers, dispersants, stabilizers or other materials which may be present.
- a weight ratio of flumetsulam to DCOIT is from 15:1 1 to 1:12, preferably from 12:1 1 to 1:12, preferably from 12:1 to 1:10; preferably from 10:1 to 1:10.
- a weight ratio of flumetsulam to OIT is from 7:1 to 1:4; preferably from 6:1 to 1:4; preferably from 7:1 to 1:3; preferably from 6:1 to 1:3; preferably from 5:1 to 1:3.
- a weight ratio of a weight ratio of flumetsulam to BBIT is from 15:1 to 1:12; preferably from 12:1 to 1:12; preferably from 12:1 to 1:10; preferably from 10:1 to 1:10.
- a weight ratio of diclosulam to DCOIT is from 6:1 to 1:3; preferably from 5:1 to 1:3; preferably from 6:1 to 1:2; preferably from 5:1 to 1:2.
- a weight ratio of diclosulam to OIT is from 7:1 to 1:1.5 or 1:3 to 1:6; preferably from 6:1 to 1:1.5 or 1:3 to 1:6; preferably from 5:1 to 1:1.5 or 1:3 to 1:5; preferably from 7:1 to 1:1 or 1:3 to 1:5; preferably from 6:1 to 1:1 or 1:3 to 1:5; preferably from 5:1 to 1:1 or 1:3 to 1:5; preferably from 5:1 1 to 1:1; preferably from 5:1 1 to 2:1.
- flumetsulam in combination with DCOIT and/or OIT is used to inhibit growth of fungi in an aqueous medium.
- the ratio of flumetsulam to DCOIT is from 15:1 to 1:4; preferably from 12:1 to 1:4; preferably from 12:1 to 1:3; preferably from 10:1 to 1:3; preferably from 10:1 to 1:2.
- the ratio of flumetsulam to OIT is from from 7:1 to 1:4; preferably from 6:1 to 1:4; preferably from 7:1 to 1:3; preferably from 6:1 to 1:3; preferably from 5:1 to 1:3.
- the antimicrobial compositions described above may contain more than one of the listed isothiazolone biocides and/or other biocides.
- the antimicrobial combinations of this invention are incorporated into liquid compositions, especially dispersions of polymers in aqueous media.
- the biocide combinations are particularly useful in preservation of building materials, e.g., adhesives, caulk, joint compound, sealant, wallboard, etc), paints, coatings, polymers, plastics, synthetic and natural rubber, paper products, fiberglass sheets, insulation, exterior insulating finishing systems, roofing and flooring felts, building plasters, wood products and wood-plastic composites.
- the antimicrobial compositions are latex paints or other liquid coating compositions containing the biocide combinations disclosed herein.
- the biocide combinations are useful for preservation of the dry film coating resulting after application of a paint or other liquid coating composition.
- the antimicrobial composition is an acrylic latex paint comprising one or more of the biocide combinations disclosed herein, or the dry film coating resulting from application of the paint to a surface.
- the amount of the biocide combinations of the present invention to control the growth of microorganisms is from 100 ppm to 10,000 ppm active ingredient.
- the active ingredients of the composition are present in an amount of at least 300 ppm, preferably at least 500 ppm, preferably at least 600 ppm, preferably at least 700 ppm.
- the active ingredients of the composition are present in an amount of no more than 8,000 ppm, preferably no more than 6,000 ppm, preferably no more than 5,000 ppm, preferably no more than 4,000 ppm, preferably no more than 3,000 ppm, preferably no more than 2500 ppm, preferably no more than 2,000 ppm, preferably no more than 1,800 ppm, preferably no more than 1,600 ppm.
- Concentrations mentioned above are in a liquid composition containing the biocide combinations; biocide levels in the dry film coating will be higher.
- the present invention also encompasses a method for preventing microbial growth in building materials, especially in dry film coatings, by incorporating any of the claimed biocide combinations into the materials.
- Sample preparation A single or blend of biocides was post added into white acrylic latex paint free of biocides to give a maximum total active ingredient/s concentration tested. This paint was then diluted with a biocide free acrylic latex paint to give targeted concentrations for the testing. Depending on the type of biocide blends tested, the total biocides concentrations varies from 200 to 5000 ppm. After biocides addition or dilution, each sample was hand mixed for at least a minute until uniformity is achieved.
- Each of the paint samples as well as a control sample (containing no biocide) were used to prepare films on black plastic-vinyl chloride/ acetate copolymer panels (LENETA, Mahwah, NJ) using a 3 mil (0.0762 mm) bird bar applicator. The panels were thoroughly dried for at least 2 days avoiding direct exposure to sunlight. Square discs (0.5 inch 2 ; 1.27 cm 2 ) were cut out from each panel and were used as the substrate for fungal and algal efficacy tests. This sample size allowed for an agar border when the sample disc was placed into the well of the test plate. Each sample was tested in duplicate
- Test conditions The appropriate media (BOLD'S 3N for Chlorophytes, BG-11 for Cyanobacteria, and PDA for fungi) were used to support microbial growth.
- the test plates were maintained at room temp (25°C - 26°C), in a cycled light-dark environment, for four weeks for algae. Plates for fungal challenge tests were maintained at 30C for four weeks. At the end of the incubation period the samples were scored for percent area covered by visible microbial growth.
- Algal inoculum Organisms abbreviation Type Medium for testing Gloeocapsa sp. Gs ATCC 29159 Unicellular, Colonial Cyanobacteria BG-11 Oscillatoria sp.
- ASTM 5589 is a standard accelerated test method for determining resistance of various coatings (including paints) to algal defacement. To accommodate for high-throughput screening, this method was scaled down from petri plates to 6-well plates. A single coupon was placed with a pair of sterile forceps at the center of the agar plug (on top) with the painted surface facing upwards. Algal inoculums were prepared by mixing equal concentrations (1X 10 6 cfu/ml) and equal volumes (depending on number of samples to be inoculated) of like growing organisms.
- Each well that contains a tested coupon was inoculated with 400 ⁇ l of organism mixture (1X 10 6 cfu/ml) making sure that the whole surface (paint film as well as the agar surrounding it) was evenly covered.
- the plates were incubated at room temp (25°C - 26°C) with cyclic exposure to light (OTT-Lite model # OTL4012P, 40Watt, 26KLumen) and dark phases, for a period of four weeks.
- the total area covered was evaluated at the end of each week according to percent area covered in 5% increments.
- ASTM 5590 is a standard accelerated test method for determining resistance of various coatings (including paints) to fungal defacement. To accommodate for high-throughput screening, this method was scaled down from petri plates to 6-well plates. To set up the test, an agar plug was placed at the bottom of each well of the sterile 6-well plate. A single coupon was placed with a pair of sterile forceps at the center of the agar plug (on top) with the painted surface facing upwards. Fungal inoculums were prepared by mixing equal concentrations (1X 10 6 cfu/ml) and equal volumes (depending on number of samples to be tested) of like growing organisms.
- SI is calculated based on F.C.Kull et. Al. method (Applied Microbiology, Vol. 9 (1961 ). In this study, SI was calculated based on the following formula with the minimum inhibitory concentration chosen based on the percent inhibitory exhibited by the individual biocide against each microorganisms tested.
- SI Qa/QA+Qb/QB
- Qa the concentration of Biocide A in the blend
- QA The concentration of Biocide A as the only biocide
- Qb The concentration of Biocide B in the blend
- QB The concentration of Biocide B as the only biocide
- SI value of ⁇ 1 in the formula indicates a synergism of the blended biocides exists.
Abstract
Description
- This invention relates to combinations of biocides, the combinations having greater activity than would be observed for the individual antimicrobial compounds.
- Use of combinations of at least two antimicrobial compounds can broaden potential markets, reduce use concentrations and costs, and reduce waste. In some cases, commercial antimicrobial compounds cannot provide effective control of microorganisms, even at high use concentrations, due to weak activity against certain types of microorganisms, e.g., those resistant to some antimicrobial compounds. Combinations of different antimicrobial compounds are sometimes used to provide overall control of microorganisms in a particular end use environment. For example,
U.S. Pat. No. 5,591,760 discloses a combination of 3-iodo-2-propynyl-butylcarbamate (IPBC) and 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one (DCOIT), but this reference does not suggest any of the combinations claimed herein. Moreover, there is a need for additional combinations of antimicrobial compounds having enhanced activity against various strains of microorganisms to provide effective control of the microorganisms, especially in dry film coatings. The problem addressed by this invention is to provide such additional combinations of antimicrobial compounds. - The present invention is directed to a synergistic antimicrobial composition comprising: (a) flumetsulam; and (b) an isothiazolone biocide selected from the group consisting of 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one, 2-n-octyl-4-isothiazolin-3-one (OIT) and N-n-butyl-1,2-benzisothiazofin-3-one (BBIT); wherein a weight ratio of flumetsulam to 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one is from 15:1 to 1:15, a weight ratio of flumetsulam to 2-n-octyl-4-isothiazolin-3-one is from 7:1 to 1:5 and a weight ratio of flumetsulam to N-n-butyl-1,2-benzisothiazolin-3-one is from 15:1 to 1:15.
- The present invention is further directed to a synergistic antimicrobial composition comprising: (a) diclosulam; and (b) an isothiazolone biocide selected from the group consisting of 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one and 2-n-octyl-4-isothiazolin-3-one; wherein a weight ratio of diclosulam to 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one is from 7:1 to 1:3 and a weight ratio of diclosulam to 2-n-octyl-4-isothiazolin-3-one is from 7:1 to 1:1.5 or 1:3 to 1:7.
- As used herein, the following terms have the designated definitions, unless the context clearly indicates otherwise. Fluometsulam is N-(2,6-difluorophenyl)-5-methyl[1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide. Diclosulam is N-(2,6-dichlorophenyl)-5-ethoxy-7-fluoro[1,2,4]triazolo[1,5-c]pyrimidine-2-sulfonamide. The term "antimicrobial compound" refers to a compound capable of inhibiting the growth of or controlling the growth of microorganisms; antimicrobial compounds include bactericides, bacteristats, fungicides, fungistats, algaecides and algistats, depending on the dose level applied, system conditions and the level of microbial control desired. The term "microorganism" includes, for example, fungi (such as yeast and mold), bacteria and algae. The following abbreviations are used throughout the specification: ppm = parts per million by weight (weight/weight), mL = milliliter, ATCC = American Type Culture Collection, and MIC = minimum inhibitory concentration. Unless otherwise specified, temperatures are in degrees centigrade (°C), and references to percentages are by weight (wt%). Percentages of antimicrobial compounds in the composition of this invention are based on the total weight of active ingredients in the composition, i.e., the antimicrobial compounds themselves, exclusive of any amounts of solvents, carriers, dispersants, stabilizers or other materials which may be present.
- Preferably, when the antimicrobial composition comprises flumetsulam and DCOIT, a weight ratio of flumetsulam to DCOIT is from 15:1 1 to 1:12, preferably from 12:1 1 to 1:12, preferably from 12:1 to 1:10; preferably from 10:1 to 1:10.
- Preferably, when the antimicrobial composition comprises flumetsulam and OIT, a weight ratio of flumetsulam to OIT is from 7:1 to 1:4; preferably from 6:1 to 1:4; preferably from 7:1 to 1:3; preferably from 6:1 to 1:3; preferably from 5:1 to 1:3.
- Preferably, when the antimicrobial composition comprises flumetsulam and BBIT, a weight ratio of a weight ratio of flumetsulam to BBIT is from 15:1 to 1:12; preferably from 12:1 to 1:12; preferably from 12:1 to 1:10; preferably from 10:1 to 1:10.
- Preferably, when the antimicrobial composition comprises diclosulam and DCOIT, a weight ratio of diclosulam to DCOIT is from 6:1 to 1:3; preferably from 5:1 to 1:3; preferably from 6:1 to 1:2; preferably from 5:1 to 1:2.
- Preferably, when the antimicrobial composition comprises diclosulam and OIT, a weight ratio of diclosulam to OIT is from 7:1 to 1:1.5 or 1:3 to 1:6; preferably from 6:1 to 1:1.5 or 1:3 to 1:6; preferably from 5:1 to 1:1.5 or 1:3 to 1:5; preferably from 7:1 to 1:1 or 1:3 to 1:5; preferably from 6:1 to 1:1 or 1:3 to 1:5; preferably from 5:1 to 1:1 or 1:3 to 1:5; preferably from 5:1 1 to 1:1; preferably from 5:1 1 to 2:1.
- Preferably, flumetsulam in combination with DCOIT and/or OIT is used to inhibit growth of fungi in an aqueous medium. Preferably, the ratio of flumetsulam to DCOIT is from 15:1 to 1:4; preferably from 12:1 to 1:4; preferably from 12:1 to 1:3; preferably from 10:1 to 1:3; preferably from 10:1 to 1:2. Preferably, the ratio of flumetsulam to OIT is from from 7:1 to 1:4; preferably from 6:1 to 1:4; preferably from 7:1 to 1:3; preferably from 6:1 to 1:3; preferably from 5:1 to 1:3.
- The antimicrobial compositions described above may contain more than one of the listed isothiazolone biocides and/or other biocides.
- Preferably, the antimicrobial combinations of this invention are incorporated into liquid compositions, especially dispersions of polymers in aqueous media. The biocide combinations are particularly useful in preservation of building materials, e.g., adhesives, caulk, joint compound, sealant, wallboard, etc), paints, coatings, polymers, plastics, synthetic and natural rubber, paper products, fiberglass sheets, insulation, exterior insulating finishing systems, roofing and flooring felts, building plasters, wood products and wood-plastic composites. Preferably, the antimicrobial compositions are latex paints or other liquid coating compositions containing the biocide combinations disclosed herein. The biocide combinations are useful for preservation of the dry film coating resulting after application of a paint or other liquid coating composition. Preferably, the antimicrobial composition is an acrylic latex paint comprising one or more of the biocide combinations disclosed herein, or the dry film coating resulting from application of the paint to a surface.
- Typically, the amount of the biocide combinations of the present invention to control the growth of microorganisms is from 100 ppm to 10,000 ppm active ingredient. Preferably, the active ingredients of the composition are present in an amount of at least 300 ppm, preferably at least 500 ppm, preferably at least 600 ppm, preferably at least 700 ppm. Preferably, the active ingredients of the composition are present in an amount of no more than 8,000 ppm, preferably no more than 6,000 ppm, preferably no more than 5,000 ppm, preferably no more than 4,000 ppm, preferably no more than 3,000 ppm, preferably no more than 2500 ppm, preferably no more than 2,000 ppm, preferably no more than 1,800 ppm, preferably no more than 1,600 ppm. Concentrations mentioned above are in a liquid composition containing the biocide combinations; biocide levels in the dry film coating will be higher.
- The present invention also encompasses a method for preventing microbial growth in building materials, especially in dry film coatings, by incorporating any of the claimed biocide combinations into the materials.
- Sample preparation: A single or blend of biocides was post added into white acrylic latex paint free of biocides to give a maximum total active ingredient/s concentration tested. This paint was then diluted with a biocide free acrylic latex paint to give targeted concentrations for the testing. Depending on the type of biocide blends tested, the total biocides concentrations varies from 200 to 5000 ppm. After biocides addition or dilution, each sample was hand mixed for at least a minute until uniformity is achieved. Each of the paint samples as well as a control sample (containing no biocide) were used to prepare films on black plastic-vinyl chloride/ acetate copolymer panels (LENETA, Mahwah, NJ) using a 3 mil (0.0762 mm) bird bar applicator. The panels were thoroughly dried for at least 2 days avoiding direct exposure to sunlight. Square discs (0.5 inch2; 1.27 cm2) were cut out from each panel and were used as the substrate for fungal and algal efficacy tests. This sample size allowed for an agar border when the sample disc was placed into the well of the test plate. Each sample was tested in duplicate
- Test conditions: The appropriate media (BOLD'S 3N for Chlorophytes, BG-11 for Cyanobacteria, and PDA for fungi) were used to support microbial growth. The test plates were maintained at room temp (25°C - 26°C), in a cycled light-dark environment, for four weeks for algae. Plates for fungal challenge tests were maintained at 30C for four weeks. At the end of the incubation period the samples were scored for percent area covered by visible microbial growth.
Algal inoculum Organisms abbreviation Type Medium for testing Gloeocapsa sp. Gs ATCC 29159 Unicellular, Colonial Cyanobacteria BG-11 Oscillatoria sp. Os ATCC 29135 Filamentous Cyanobacteria BG-11 Nostoc commune Nc CCAP 1453/29 Unicellular, Cenobial Chlorophyte Bold Trentepohlia aurea + Trentepohlia odorata Ta+To UTEX LB 429 + CCAP 483/4 Filamentous Chlorophyte Bold Chlorella sp. UTEX + Chlorella kessleri Cs+Ck ATCC 30582 + ATCC 11468 Unicellular Chlorophyte Bold Calothrix parientina Cp UTEX LB 1952 Filamentous Cyanobacteria Bold Fungal inoculum Organisms abbreviation ATCC# Medium for Growth and Testing Aspergillus niger An 9642 PDA Penicillium funiculosum Pf 11797 PDA Cladosporium herbarum Ch 11281 PDA Aureobasidium pullulans Ap 9348 PDA Trichoderma viride Tv 32630 PDA Alternaria alternata Aa 20084 PDA - ASTM 5589 is a standard accelerated test method for determining resistance of various coatings (including paints) to algal defacement. To accommodate for high-throughput screening, this method was scaled down from petri plates to 6-well plates. A single coupon was placed with a pair of sterile forceps at the center of the agar plug (on top) with the painted surface facing upwards. Algal inoculums were prepared by mixing equal concentrations (1X 106 cfu/ml) and equal volumes (depending on number of samples to be inoculated) of like growing organisms.
- In Flumetsulam + various IT synergy study, three pool of mixed algae were prepared as the test inoculum, Gloeocapsa sp. and Oscillatoria sp.a mix of cyanobacteria grown on BG-11 media; Chlorella sp., Chlorella kessleri, and Nostoc commune are unicellular chlorphytes that were mixed and grown on Bold media; Trentepohlia aurea, Tretepohlia odorata, and Calotrix parientina are filamentous algae that were mixed and grown on Bold media.
- In Diclosulam + various IT synergy study, only two pools of mixed algae were prepared; Gloeocapsa sp. and Oscillatoria sp. grown on BG-11 media and Chlorella sp., Chlorella kessleri, Nostoc commune, Trentepohlia aurea, Tretepohlia odorata, and Calotrix parientina grown on Bold media
- Each well that contains a tested coupon was inoculated with 400 µl of organism mixture (1X 106 cfu/ml) making sure that the whole surface (paint film as well as the agar surrounding it) was evenly covered. The plates were incubated at room temp (25°C - 26°C) with cyclic exposure to light (OTT-Lite model # OTL4012P, 40Watt, 26KLumen) and dark phases, for a period of four weeks. The total area covered was evaluated at the end of each week according to percent area covered in 5% increments.
- ASTM 5590 is a standard accelerated test method for determining resistance of various coatings (including paints) to fungal defacement. To accommodate for high-throughput screening, this method was scaled down from petri plates to 6-well plates. To set up the test, an agar plug was placed at the bottom of each well of the sterile 6-well plate. A single coupon was placed with a pair of sterile forceps at the center of the agar plug (on top) with the painted surface facing upwards. Fungal inoculums were prepared by mixing equal concentrations (1X 106 cfu/ml) and equal volumes (depending on number of samples to be tested) of like growing organisms. For Flumetsulam + various IT synergy study, three pools of mixed fungi were prepared as the test inoculum. Cladosporium herbarum was mixed with Aureobasidium pullulans; Aspergillus niger was mixed with Penicillium funiculosum and Alternaria alternata was mixed with Trichoderma viride. For Diclosulam + various IT synergy study, all above fungi were mixed as a single pool. Each well was inoculated with 400 µl of organism mixture (1X 106 cfu/ml) making sure that the whole surface (paint film as well as the agar surrounding it) was evenly covered. The plates were incubated at 30°C in presence of moisture, for a period of four weeks. The total percent area covered was evaluated and recorded at the end of each week after the 2nd week and recorded in increments of 5%.
- The SI is calculated based on F.C.Kull et. Al. method (Applied Microbiology, Vol. 9 (1961). In this study, SI was calculated based on the following formula with the minimum inhibitory concentration chosen based on the percent inhibitory exhibited by the individual biocide against each microorganisms tested.
SI= Qa/QA+Qb/QB
Qa = the concentration of Biocide A in the blend
QA = The concentration of Biocide A as the only biocide
Qb = The concentration of Biocide B in the blend
QB = The concentration of Biocide B as the only biocide - SI value of < 1 in the formula indicates a synergism of the blended biocides exists.
- Note: If any of the active with maximum concentration tested did not exhibit some inhibition, this maximum concentration is used to calculate the estimated SI and a sign of less than (<) is included to take into account that higher concentration of the active is needed to achieve the targeted inhibition
- NE = no end point at the concentration tested that will meet the percent inhibition criteria set in each SI calculation
Flumetsulam:DCOIT synergy study AaTv AnPf ApCh Cp+To+Ta Cs+Ck+Nc Gs+Os 1Flumet:1DCOIT Total conc, ppm 1150 575 575 575 575 575 % inhibition 100 95 100 100 100 100 SI < 1.16 < 0.58 0.6 2.7 0.4 2.1 2Flumet:1DCOIT Total conc, ppm 2588 862.5 862.5 431.25 431.25 431.25 % inhibition 100.0 90 100 95 100 100 SI <1.99 < 0.66 0.7 1.7 0.3 1.1 3Flumet:1DCOIT Total conc, ppm 1000 1000 1000 1000 1000 1000 % inhibition 100 100 100 100 95 1000 SI < 0.65 < 0.65 0.7 3.5 0.7 2.0 5Flumet:1DCOIT Total conc, ppm 750 1500 750 750 750 750 % inhibition 80 90 95 100 100 100 SI < 0.40 < 0.7 0.4 2.3 0.5 1.1 10Flumet:1DCOIT Total conc, ppm 1650 1650 825 825 1650 825 % inhibition 100 90 100 100 100 100 SI < 0.69 < 0.69 0.3 2.2 1.0 0.8 1Flumet:2DCOIT Total conc, ppm 888 444 444 444 444 444 % inhibition 90 100 100 95 100 100 SI <1.11 < 0.56 0.6 2.4 0.3 2.1 1Flumet:5DCOIT Total conc, ppm 1380 690 690 690 690 690 % inhibition 100 100 100 100 100 100 SI < 2.1 < 1.0 1.0 4.3 0.6 4.0 1Flumet:10DCOIT Total conc, ppm 632.5 632.5 632.5 632.5 632.5 632.5 % inhibition 90 100 100 100 100 100 SI < 1.01 < 1.0 1.0 4.1 0.5 4.0 Flumetsulam Total conc, ppm 3500 3500 3500 437.5 1750 2625 % inhibition 0 0 90 95 100 100 DCOIT Total conc, ppm 575 575 862.5 143.75 1150 143.75 % inhibition 85 100 100 100 100 100 Flumetsulam :OIT Synergy study AaTv AnPf ApCh Cp+To+Ta Cs+Ck+Nc Gs+Os 1Flumet:1OIT Total conc, ppm 750 750 750 750 750 750 % inhibition 100 100 100 100 100 100 SI < 0.50 0.9 < 0.5 1.7 1.0 1.7 2Flumet:1OIT Total conc, ppm 750 750 750 750 750 750 % inhibition 100.0 100 95 100 100 90 SI <0.4 0.7 < 0.4 1.7 0.7 1.7 3Flumet:1OIT Total conc, ppm 750 750 1500 750 750 750 % inhibition 100 95 95 95 100 100 SI < 0.40 0.6 < 0.70 1.7 0.6 7.0 5Flumet:1OIT Total conc, ppm 1800 687.5 1800 687.5 687.5 687.5 % inhibition 100 95 100 90 100 95 SI < 0.80 0.3 < 0.80 1.6 0.4 1.6 1Flumet:3OIT Total conc, ppm 600 1200 600 600 600 600 % inhibition 100 100 90 100 100 95 SI < 0.5 2.1 < 0.50 1.3 1.1 1.3 1Flumet:2OIT Total conc, ppm 675 1350 675 675 675 675 % inhibition 100 100 100 100 100 100 SI <0.60 2.1 < 0.60 1.5 1.1 1.5 Flumetsulam Total conc, ppm 3500 3500 3500 437.5 2625 437.5 % inhibition 0 65 0 60 65 90 OIT Total conc, ppm 900 450 900 450 450 450 % inhibition 100 100 80 100 100 100 Flumetsulam: BBIT synergy study AaTv AnPf ApCh Cp+To+Ta Cs+Ck+Nc Gs+Os 1Flumet:1BBIT Total conc, ppm NE 575 575 NE 1150 575 % inhibition 60 80 80 100 SI < 0.33 < 0.58 < 0.66 < 0.33 2Flumet:1BBIT Total conc, ppm NE 431 1725 NE 1725 1725 % inhibition 80 60 80 100 SI < 0.21 < 1.32 < 0.83 < 0.83 3Flumet:1BBIT Total conc, ppm NE 1000 NE NE 1000 3000 % inhibition 80 60 80 SI < 0.43 < 0.43 < 1.30 5Flumet:1BBIT Total conc, ppm NE 1500 NE 3000 3000 3000 % inhibition 80 60 60 60 SI < 0.57 1.2 <1.14 <1.15 10Flumet:1BBIT Total conc, ppm NE 825 NE 2475 1650 825 % inhibition 80 100 80 100 SI < 0.28 < 0.84 < 0.56 <0.28 1Flumet:1.8BBIT Total conc, ppm NE 887.5 NE NE 887.5 887.5 % inhibition 80 60 60 SI < 0.59 < 0.59 < 0.59 1Flumet:5 BBIT Total conc, ppm NE 1035 NE NE 690 690 % inhibition 80 100 60 SI < 0.8 < 0.53 < 0.53 1Flumet:10 BBIT Total conc, ppm NE 949 NE NE 949 632.5 % inhibition 80 80 80 SI < 0.77 < 0.77 < 0.52 Flumetsulam Total conc, ppm 3500 3500 3500 3500 3500 3500 % inhibition 0 0 0 0 0 0 BBIT Total conc, ppm 1150 1150 575 1150 1150 1150 % inhibition 0 0 80 0 0 0 Synergy study of Diclosulam and DCOIT Ratios Aa+Tv+An+Pf+Ap+Ch Cp+To+Ta+Cs+Ck+Nc Gs+Os 1Diclosulam:1DCOIT Total conc, ppm 575 575 575 % inhibition 85 100 100 SI 0.5 1.1 1.1 1Dicolosulam:2 DCOIT Total conc, ppm 887.5 443.75 443.75 % inhibition 100 100 100 SI 1.0 0.8 0.8 1Diclosulam:5 DCOIT Total conc, ppm 690 690 690 % inhibition 95 100 100 SI 1.0 1.1 1.1 1Diclosulam:10 DCOIT Total conc, ppm 632.5 632.5 632.5 % inhibition 95 100 100 SI 0.9 1.0 1.0 2Diclosulam:1 DCOIT Total conc, ppm 862.5 443.75 443.75 % inhibition 87.5 100 100 SI 0.6 0.9 0.9 5Diclosulam: DCOIT Total conc, ppm 1500 750 750 % inhibition 85 100 100 SI 0.8 1.6 1.6 10Diclosulam:1 DCOIT Total conc, ppm 3300 825 825 % inhibition 90 100 100 SI 1.3 1.8 1.8 Diclosulam Total conc, ppm 3500 437.5 437.5 % inhibition 40 100 100 DCOIT Total conc, ppm 625 625 625 % inhibition 80 100 100 Synergy study of Diclosulam + OIT Ratios Aa+Tv+An+Pf+Ap+Ch Cp+To+Ta+Cs+Ck+Nc Gs+Os 1Diclosulam:1 OIT Total conc, ppm 750 2250 750 % inhibition 100 100 100 SI < 0.9 3.8 1.3 2Dicolosulam:1 OIT Total conc, ppm 750 2250 1500 % inhibition 80 100 100 SI < 0.7 4.3 2.8 3Diclosulam:1OIT Total conc, ppm 750 2250 750 % inhibition 100 100 100 SI < 0.6 4.5 1.5 5Diclosulam:1OIT Total conc, ppm 687.5 2700 687.5 % inhibition 100 80 100 SI < 0.3 5.6 1.5 1Diclosulam:3OIT Total conc, ppm 600 600 600 % inhibition 100 100 100 SI < 1.0 1.0 0.8 1Diclosulam:2 OIT Total conc, ppm 675 675 675 % inhibition 100 100 100 SI < 1.1 1.0 1.0 1Diclosulam:5OIT Total conc, ppm 540 1080 540 % inhibition 100 100 100 SI 1.0 1.4 0.7 1Diclosulam:10 OIT Total conc, ppm 990 990 990 % inhibition 100 100 100 SI < 2.0 1.2 1.2 Diclosulam Total conc, ppm 3500 437.5 437.5 % inhibition 0 100 100 OIT Total conc, ppm 450 900 900 % inhibition 100 100 100
Claims (9)
- A synergistic antimicrobial composition comprising: (a) flumetsulam; and (b) an isothiazolone biocide selected from the group consisting of 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one, 2-n-octyl-4-isothiazolin-3-one and N-n-butyl-1,2-benzisothiazolin-3-one; wherein a weight ratio of flumetsulam to 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one is from 15:1 1 to 1:15, a weight ratio of flumetsulam to 2-n-octyl-4-isothiazolin-3-one is from 7:1 to 1:5 and a weight ratio of flumetsulam to N-n-butyl-1,2-benzisothiazofin-3-one is from 15:1 to 1:15.
- The composition of claim 1 in which the isothiazolone biocide is 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one and the weight ratio of flumetsulam to 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one is from 10:1 to 1:10.
- The composition of claim 1 in which the isothiazolone biocide is 2-n-octyl-4-isothiazolin-3-one and the weight ratio of flumetsulam to 2-n-octyl-4-isothiazolin-3-one is from 5:1 to 1:3.
- The composition of claim 1 the isothiazolone biocide is N-n-butyl-1,2-benzisothiazolin-3-one and the weight ratio of flumetsulam to N-n-butyl-1,2-benzisothiazolin-3-one is from 10:1 to 1:10.
- A synergistic antimicrobial composition comprising: (a) diclosulam; and (b) an isothiazolone biocide selected from the group consisting of 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one and 2-n-octyl-4-isothiazolin-3-one; wherein a weight ratio of diclosulam to 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one is from 7:1 to 1:3 and a weight ratio of diclosulam to 2-n-octyl-4-isothiazolin-3-one is from 7:1 to 1:1.5 or 1:3 to 1:7.
- The composition of claim 5 in which the isothiazolone biocide is 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one and the weight ratio of diclosulam to 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one is from 5:1 to 1:2.
- The composition of claim 5 in which the isothiazolone biocide is 2-n-octyl-4-isothiazolin-3-one and the weight ratio of diclosulam to 2-n-octyl-4-isothiazolin-3-one is from 5:1 to 1:1 or 1:3 to 1:5.
- The composition of claim 1 which is a building material, paint, coating, polymer, plastic, synthetic or natural rubber, paper product, fiberglass sheet, insulation, exterior insulating finishing system, roofing and flooring felt, building plaster, wood product or wood-plastic composite.
- The composition of claim 5 which is a building material, paint, coating, polymer, plastic, synthetic or natural rubber, paper product, fiberglass sheet, insulation, exterior insulating finishing system, roofing and flooring felt, building plaster, wood product or wood-plastic composite.
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PL11184073T PL2471366T3 (en) | 2010-11-04 | 2011-10-06 | Synergistic combination of flumetsulam or diclosulam with isothiazolones |
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US41008310P | 2010-11-04 | 2010-11-04 |
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EP2471366B1 EP2471366B1 (en) | 2013-12-18 |
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US (1) | US8487098B2 (en) |
EP (1) | EP2471366B1 (en) |
JP (1) | JP5302368B2 (en) |
KR (1) | KR101344739B1 (en) |
CN (2) | CN102450273B (en) |
BR (1) | BRPI1105310B1 (en) |
MX (1) | MX2011011404A (en) |
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Cited By (2)
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---|---|---|---|---|
EP2468099A3 (en) * | 2010-12-22 | 2013-05-15 | Dow Global Technologies LLC | Synergistic combination of a glyphosate compound and one of DCOIT or OIT or BBIT |
WO2017137157A1 (en) * | 2016-02-12 | 2017-08-17 | Thor Gmbh | Composition containing 2-n-octylisothiazolin-3-one and 4,5-dichloro-2-n-octylisothiazolin-3-one for the production of wpc |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
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WO2014085738A1 (en) * | 2012-11-30 | 2014-06-05 | Rohm And Haas Company | Synergistic combination of lenacil and one of dcoit or oit for dry film protection |
JP2016504298A (en) * | 2012-11-30 | 2016-02-12 | ローム アンド ハース カンパニーRohm And Haas Company | A synergistic combination of zoxamide and DCOIT or OIT for dry film protection |
CN115487181B (en) * | 2020-09-07 | 2024-03-12 | 南京艾力彼兽药研究所有限公司 | Application of isothiazolinone as synergist of antibacterial drug |
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DE69216905T2 (en) * | 1991-05-03 | 1997-05-15 | Dowelanco | Herbicidal compositions with improved cereal security |
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JP2004315507A (en) | 2003-04-07 | 2004-11-11 | Rohm & Haas Co | Microbicidal composition |
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- 2011-10-06 EP EP11184073.2A patent/EP2471366B1/en not_active Not-in-force
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- 2011-10-31 US US13/285,092 patent/US8487098B2/en not_active Expired - Fee Related
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- 2011-11-03 CN CN201310364943.8A patent/CN103392714B/en not_active Expired - Fee Related
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- 2011-11-03 KR KR1020110113991A patent/KR101344739B1/en not_active IP Right Cessation
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
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EP2468099A3 (en) * | 2010-12-22 | 2013-05-15 | Dow Global Technologies LLC | Synergistic combination of a glyphosate compound and one of DCOIT or OIT or BBIT |
KR101400098B1 (en) * | 2010-12-22 | 2014-05-27 | 다우 글로벌 테크놀로지스 엘엘씨 | Synergistic combination of a glyphosate compound and one of dcoit or oit or bbit |
WO2017137157A1 (en) * | 2016-02-12 | 2017-08-17 | Thor Gmbh | Composition containing 2-n-octylisothiazolin-3-one and 4,5-dichloro-2-n-octylisothiazolin-3-one for the production of wpc |
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Publication number | Publication date |
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BRPI1105310A2 (en) | 2013-03-05 |
CN102450273B (en) | 2015-02-04 |
KR101344739B1 (en) | 2013-12-26 |
RU2011144301A (en) | 2013-05-10 |
JP5302368B2 (en) | 2013-10-02 |
EP2471366B1 (en) | 2013-12-18 |
KR20120047829A (en) | 2012-05-14 |
US20120115887A1 (en) | 2012-05-10 |
PL2471366T3 (en) | 2014-05-30 |
CN103392714B (en) | 2015-03-11 |
CN103392714A (en) | 2013-11-20 |
EP2471366A3 (en) | 2012-12-05 |
MX2011011404A (en) | 2012-05-15 |
CN102450273A (en) | 2012-05-16 |
US8487098B2 (en) | 2013-07-16 |
RU2572701C2 (en) | 2016-01-20 |
JP2012102079A (en) | 2012-05-31 |
BRPI1105310B1 (en) | 2018-01-02 |
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