EP2430164A4 - Production of recombinant proteins utilizing non-antibiotic selection methods and the incorporation of non-natural amino acids therein - Google Patents

Production of recombinant proteins utilizing non-antibiotic selection methods and the incorporation of non-natural amino acids therein

Info

Publication number
EP2430164A4
EP2430164A4 EP10775323A EP10775323A EP2430164A4 EP 2430164 A4 EP2430164 A4 EP 2430164A4 EP 10775323 A EP10775323 A EP 10775323A EP 10775323 A EP10775323 A EP 10775323A EP 2430164 A4 EP2430164 A4 EP 2430164A4
Authority
EP
European Patent Office
Prior art keywords
incorporation
production
amino acids
natural amino
recombinant proteins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP10775323A
Other languages
German (de)
French (fr)
Other versions
EP2430164A2 (en
EP2430164B1 (en
Inventor
Diane M Retallack
Lawrence C Chew
Charles H Squires
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pfenex Inc
Original Assignee
Pfenex Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pfenex Inc filed Critical Pfenex Inc
Publication of EP2430164A2 publication Critical patent/EP2430164A2/en
Publication of EP2430164A4 publication Critical patent/EP2430164A4/en
Application granted granted Critical
Publication of EP2430164B1 publication Critical patent/EP2430164B1/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/78—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
EP10775323.8A 2009-05-11 2010-05-10 Production of recombinant proteins utilizing non-antibiotic selection methods and the incorporation of non-natural amino acids therein Active EP2430164B1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US17726709P 2009-05-11 2009-05-11
PCT/US2010/034201 WO2010132341A2 (en) 2009-05-11 2010-05-10 Production of recombinant proteins utilizing non-antibiotic selection methods and the incorporation of non-natural amino acids therein

Publications (3)

Publication Number Publication Date
EP2430164A2 EP2430164A2 (en) 2012-03-21
EP2430164A4 true EP2430164A4 (en) 2012-10-31
EP2430164B1 EP2430164B1 (en) 2017-07-19

Family

ID=43085513

Family Applications (1)

Application Number Title Priority Date Filing Date
EP10775323.8A Active EP2430164B1 (en) 2009-05-11 2010-05-10 Production of recombinant proteins utilizing non-antibiotic selection methods and the incorporation of non-natural amino acids therein

Country Status (7)

Country Link
US (2) US9243253B2 (en)
EP (1) EP2430164B1 (en)
AU (2) AU2010247938B2 (en)
CA (1) CA2761578C (en)
ES (1) ES2640947T3 (en)
NZ (1) NZ596283A (en)
WO (1) WO2010132341A2 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2723872A1 (en) * 2011-06-24 2014-04-30 Algenol Biofuels Inc. Genetically enhanced cyanobacteria lacking functional genes conferring biocide resistance for the production of chemical compounds
WO2014100798A1 (en) * 2012-12-21 2014-06-26 Algenol Biofuels, Inc. Novel shuttle vector capable of transforming multiple genera of cyanobacteria
KR101660580B1 (en) * 2014-04-02 2016-09-28 프레스티지 바이오파마 피티이. 엘티디. A method for preparing an antibody by controlling a sugar content of the antibody
MX2016014824A (en) 2014-05-13 2017-03-23 Bioatla Llc Conditionally active biological proteins.
WO2016004024A1 (en) * 2014-07-02 2016-01-07 Sutro Biopharma, Inc. High growth capacity auxotrophic escherichia coli and methods of use
EP3189152A4 (en) 2014-09-03 2018-04-04 BioAtla LLC Discovering and producing conditionally active biologic proteins in the same eukaryotic cell production hosts
ES2769877T3 (en) * 2014-10-23 2020-06-29 Sanofi Sa Novel selection marker for cell transfection and protein production
EP3353313A2 (en) 2015-09-23 2018-08-01 Pfizer Inc Cells and method of cell culture
BR112018070139A2 (en) * 2016-03-29 2019-02-05 Geltor Inc protein expression in gram-negative bacteria where the ratio of periplasmic volume to cytoplasmic volume is between 0.5: 1 and 10: 1
US20200056190A1 (en) * 2017-03-16 2020-02-20 Pfizer Inc. Tyrosine prototrophy
KR102166288B1 (en) * 2019-03-06 2020-10-15 한국생명공학연구원 D-glutamate auxotrophic Escherichia coli and method of producing target product
CN110029068B (en) * 2019-04-10 2022-03-25 天津科技大学 Aspergillus niger genetic engineering strain for high yield of organic acid under low dissolved oxygen condition and application thereof
WO2020243642A2 (en) * 2019-05-31 2020-12-03 The Trustees Of Columbia University In The City Of New York Multiply auxotrophic cell line for the production of recombinant proteins and methods thereof
CN115322924B (en) * 2022-07-19 2023-11-14 济南大学 Method for synthesizing polyhydroxyalkanoate by double-bacteria mixed culture

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005052151A1 (en) * 2003-11-19 2005-06-09 Dow Global Technologies Inc. Improved protein expression systems

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4511503A (en) 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4755465A (en) 1983-04-25 1988-07-05 Genentech, Inc. Secretion of correctly processed human growth hormone in E. coli and Pseudomonas
US4680264A (en) 1983-07-01 1987-07-14 Lubrizol Genetics, Inc. Class II mobilizable gram-negative plasmid
US5281532A (en) 1983-07-27 1994-01-25 Mycogen Corporation Pseudomas hosts transformed with bacillus endotoxin genes
DK594084A (en) 1984-12-12 1986-06-13 Novo Industri As PROCEDURE FOR STABILIZING EXTRA-CHROMOSOMAL ELEMENTS IN BACTERIA IN CULTURE
US4695455A (en) 1985-01-22 1987-09-22 Mycogen Corporation Cellular encapsulation of pesticides produced by expression of heterologous genes
US4695462A (en) 1985-06-28 1987-09-22 Mycogen Corporation Cellular encapsulation of biological pesticides
GB8517071D0 (en) 1985-07-05 1985-08-14 Hoffmann La Roche Gram-positive expression control sequence
US5128130A (en) 1988-01-22 1992-07-07 Mycogen Corporation Hybrid Bacillus thuringiensis gene, plasmid and transformed Pseudomonas fluorescens
US5055294A (en) 1988-03-03 1991-10-08 Mycogen Corporation Chimeric bacillus thuringiensis crystal protein gene comprising hd-73 and berliner 1715 toxin genes, transformed and expressed in pseudomonas fluorescens
US5169760A (en) 1989-07-27 1992-12-08 Mycogen Corporation Method, vectors, and host cells for the control of expression of heterologous genes from lac operated promoters
US6566121B1 (en) 1991-06-13 2003-05-20 Albert Einstein College Of Medicine Of Yeshiva University Insertional mutations in mycobacteria
US5691185A (en) 1993-10-08 1997-11-25 Chr. Hansen A/S Lactic acid bacterial suppressor mutants and their use as selective markers and as means of containment in lactic acid bacteria
US5527883A (en) 1994-05-06 1996-06-18 Mycogen Corporation Delta-endotoxin expression in pseudomonas fluorescens
US6291245B1 (en) 1998-07-15 2001-09-18 Roche Diagnostics Gmbh Host-vector system
US6413768B1 (en) 1998-12-02 2002-07-02 University Of Maryland Expression plasmids
DK2322631T3 (en) 2001-04-19 2015-01-12 Scripps Research Inst Methods and compositions for the production of orthogonal tRNA-aminoacyl-tRNA syntetasepar
CN1678624B (en) 2002-07-03 2011-10-26 陶氏环球技术公司 Benzoate- and anthranilate-inducible promoters
JP2008525032A (en) 2004-12-22 2008-07-17 アンブレツクス・インコーポレイテツド Methods for expressing and purifying recombinant human growth hormone
JP2009519942A (en) 2005-12-14 2009-05-21 アンブルックス，インコーポレイテッド Compositions comprising unnatural amino acids and polypeptides, methods relating thereto, and uses thereof
CN101528914B (en) 2006-09-08 2014-12-03 Ambrx公司 Site specific incorporation of non-natural amino acids by vertebrate cells

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005052151A1 (en) * 2003-11-19 2005-06-09 Dow Global Technologies Inc. Improved protein expression systems

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
ALBERT J. DE GRAAF ET AL: "Nonnatural Amino Acids for Site-Specific Protein Conjugation", BIOCONJUGATE CHEMISTRY, vol. 20, no. 7, 3 February 2009 (2009-02-03), pages 1281 - 1295, XP055025160, ISSN: 1043-1802, DOI: 10.1021/bc800294a *
HENDRICKSON TAMARA L ET AL: "Incorporation of nonnatural amino acids into proteins", ANNUAL REVIEW OF BIOCHEMISTRY, PALTO ALTO, CA, US, vol. 73, 26 March 2004 (2004-03-26), pages 147 - 176, XP009093569, ISSN: 0066-4154, DOI: 10.1146/ANNUREV.BIOCHEM.73.012803.092429 *
HODGSON D R W ET AL: "THE SYNTHESIS OF PEPTIDES AND PROTEINS CONTAINING NON-NATURAL AMINO ACIDS", CHEMICAL SOCIETY REVIEWS, CHEMICAL SOCIETY, LONDON, GB, vol. 33, no. 7, 1 January 2004 (2004-01-01), pages 422 - 430, XP008067398, ISSN: 0306-0012 *
K. L. KIICK: "Incorporation of azides into recombinant proteins for chemoselective modification by the Staudinger ligation (Supporting information)", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 99, no. 1, 18 December 2001 (2001-12-18), pages 1 - 3, XP055036642, ISSN: 0027-8424, DOI: 10.1073/pnas.012583299 *
KIICK K L ET AL: "Incorporation of azides into recombinant proteins for chemoselective modification by the Staudinger ligation", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, US, vol. 99, no. 1, 8 January 2002 (2002-01-08), pages 19 - 24, XP002904363, ISSN: 0027-8424, DOI: 10.1073/PNAS.012583299 *
See also references of WO2010132341A2 *

Also Published As

Publication number Publication date
US20160208268A1 (en) 2016-07-21
AU2016203224B2 (en) 2018-02-15
EP2430164A2 (en) 2012-03-21
US9708616B2 (en) 2017-07-18
CA2761578A1 (en) 2010-11-18
CA2761578C (en) 2019-06-18
US9243253B2 (en) 2016-01-26
EP2430164B1 (en) 2017-07-19
NZ596283A (en) 2013-08-30
ES2640947T3 (en) 2017-11-07
WO2010132341A3 (en) 2011-04-21
AU2010247938B2 (en) 2016-02-18
WO2010132341A2 (en) 2010-11-18
AU2010247938A1 (en) 2011-11-24
AU2016203224A1 (en) 2016-06-09
US20120115187A1 (en) 2012-05-10

Similar Documents

Publication Publication Date Title
EP2430164A4 (en) Production of recombinant proteins utilizing non-antibiotic selection methods and the incorporation of non-natural amino acids therein
IL215348A0 (en) Novel dicarboxylic acid linked amino acid and peptide prodrugs of opioids and uses thereof
IL216619A0 (en) Recombinant production of peptides
EP2547771A4 (en) Production of proteins and polypeptides
EP2250278A4 (en) Methods and compositions related to peptides and proteins with c-terminal elements cross-reference to related applications
IL230918A0 (en) Modified proteins and peptides
PL2552242T3 (en) Low protein infant formula with increased essential amino acids
HK1200489A1 (en) Recombinant human naglu protein and uses thereof naglu
EP2593469A4 (en) Modified polypeptides and proteins and uses thereof
EP2494053A4 (en) Methods for the production of recombinant proteins with improved secretion efficiencies
IL187191A0 (en) Incorporation of non-naturally encoded amino acids into proteins
EP2651964A4 (en) Croos-linked peptides and proteins, methods of making same, and uses thereof
EP2350286A4 (en) Recombinant nell protein production
IL232645A0 (en) Recombinant proteins and their therapeutic uses
IL216543A (en) Methods for improving the production of a recombinant human wild-type acid-beta-glucosidase protein
PL1998790T3 (en) Compositions containing peptides with non-natural amino acids and methods of use
EP2408926A4 (en) Production of recombinant proteins in ciliates and uses thereof
EP2968469A4 (en) Peptides comprising non-natural amino acids and methods of making and using the same
EP2406276A4 (en) Methods of renaturation of recombinant proteins
EP2456910A4 (en) Differentiation of isobaric amino acids and other species
EP2154251A4 (en) Expression system of recombinant proteins with enhanced yield and immunogenicity
EP2167665A4 (en) A method of enhancing recombinant protein production
EP2244725A4 (en) Novel peptides and protein and uses thereof
IL219182A0 (en) 2-amino-3-methyl-hex-5-enoic acid and its use in the production of peptides such as bacitracins
GB0903746D0 (en) Novel conjugated proteins and peptides

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20111212

AK Designated contracting states

Kind code of ref document: A2

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR

DAX Request for extension of the european patent (deleted)
A4 Supplementary search report drawn up and despatched

Effective date: 20121004

RIC1 Information provided on ipc code assigned before grant

Ipc: C12N 15/65 20060101AFI20120927BHEP

Ipc: C12P 21/04 20060101ALI20120927BHEP

17Q First examination report despatched

Effective date: 20140401

GRAP Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOSNIGR1

INTG Intention to grant announced

Effective date: 20161025

GRAS Grant fee paid

Free format text: ORIGINAL CODE: EPIDOSNIGR3

GRAJ Information related to disapproval of communication of intention to grant by the applicant or resumption of examination proceedings by the epo deleted

Free format text: ORIGINAL CODE: EPIDOSDIGR1

GRAL Information related to payment of fee for publishing/printing deleted

Free format text: ORIGINAL CODE: EPIDOSDIGR3

GRAP Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOSNIGR1

INTC Intention to grant announced (deleted)
INTG Intention to grant announced

Effective date: 20170330

GRAA (expected) grant

Free format text: ORIGINAL CODE: 0009210

AK Designated contracting states

Kind code of ref document: B1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR

REG Reference to a national code

Ref country code: GB

Ref legal event code: FG4D

REG Reference to a national code

Ref country code: CH

Ref legal event code: EP

REG Reference to a national code

Ref country code: IE

Ref legal event code: FG4D

REG Reference to a national code

Ref country code: AT

Ref legal event code: REF

Ref document number: 910437

Country of ref document: AT

Kind code of ref document: T

Effective date: 20170815

REG Reference to a national code

Ref country code: DE

Ref legal event code: R096

Ref document number: 602010043751

Country of ref document: DE

REG Reference to a national code

Ref country code: NL

Ref legal event code: FP

REG Reference to a national code

Ref country code: ES

Ref legal event code: FG2A

Ref document number: 2640947

Country of ref document: ES

Kind code of ref document: T3

Effective date: 20171107

RAP2 Party data changed (patent owner data changed or rights of a patent transferred)

Owner name: PFENEX, INC.

REG Reference to a national code

Ref country code: LT

Ref legal event code: MG4D

REG Reference to a national code

Ref country code: AT

Ref legal event code: MK05

Ref document number: 910437

Country of ref document: AT

Kind code of ref document: T

Effective date: 20170719

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: FI

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170719

Ref country code: AT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170719

Ref country code: SE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170719

Ref country code: NO

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20171019

Ref country code: HR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170719

Ref country code: LT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170719

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: GR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20171020

Ref country code: IS

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20171119

Ref country code: LV

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170719

Ref country code: PL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170719

Ref country code: BG

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20171019

REG Reference to a national code

Ref country code: FR

Ref legal event code: PLFP

Year of fee payment: 9

REG Reference to a national code

Ref country code: DE

Ref legal event code: R097

Ref document number: 602010043751

Country of ref document: DE

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: DK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170719

Ref country code: CZ

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170719

Ref country code: RO

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170719

PLBE No opposition filed within time limit

Free format text: ORIGINAL CODE: 0009261

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: NO OPPOSITION FILED WITHIN TIME LIMIT

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SM

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170719

Ref country code: EE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170719

Ref country code: SK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170719

26N No opposition filed

Effective date: 20180420

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SI

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170719

REG Reference to a national code

Ref country code: CH

Ref legal event code: PL

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: MC

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170719

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: LI

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20180531

Ref country code: CH

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20180531

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: LU

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20180510

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: MT

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20180510

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: TR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170719

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: HU

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT; INVALID AB INITIO

Effective date: 20100510

Ref country code: PT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170719

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: MK

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20170719

Ref country code: CY

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170719

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: AL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170719

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: IE

Payment date: 20230310

Year of fee payment: 14

Ref country code: FR

Payment date: 20230309

Year of fee payment: 14

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: GB

Payment date: 20230316

Year of fee payment: 14

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: NL

Payment date: 20230314

Year of fee payment: 14

P01 Opt-out of the competence of the unified patent court (upc) registered

Effective date: 20230527

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: IT

Payment date: 20230412

Year of fee payment: 14

Ref country code: ES

Payment date: 20230601

Year of fee payment: 14

Ref country code: DE

Payment date: 20230314

Year of fee payment: 14

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: BE

Payment date: 20230418

Year of fee payment: 14

REG Reference to a national code

Ref country code: GB

Ref legal event code: 732E

Free format text: REGISTERED BETWEEN 20240104 AND 20240110

REG Reference to a national code

Ref country code: DE

Ref legal event code: R081

Ref document number: 602010043751

Country of ref document: DE

Owner name: PELICAN TECHNOLOGY HOLDINGS, INC. (N.D. GES. D, US

Free format text: FORMER OWNER: PFENEX, INC., SAN DIEGO, CALIF., US