EP2405009A3 - Transgenic plants containing soluble cell wall polysaccharides - Google Patents

Transgenic plants containing soluble cell wall polysaccharides Download PDF

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Publication number
EP2405009A3
EP2405009A3 EP11003665A EP11003665A EP2405009A3 EP 2405009 A3 EP2405009 A3 EP 2405009A3 EP 11003665 A EP11003665 A EP 11003665A EP 11003665 A EP11003665 A EP 11003665A EP 2405009 A3 EP2405009 A3 EP 2405009A3
Authority
EP
European Patent Office
Prior art keywords
cell wall
transgenic plants
plants containing
containing soluble
soluble cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP11003665A
Other languages
German (de)
French (fr)
Other versions
EP2405009B1 (en
EP2405009A2 (en
Inventor
Ziv Shani
Oded Shoseyov
Miron Abramson
Noga Barimboim
Mara Dekel
Shaul Lapidot
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yissum Research Development Co of Hebrew University of Jerusalem
Futuragene Israel Ltd
Original Assignee
Yissum Research Development Co of Hebrew University of Jerusalem
Futuragene Israel Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yissum Research Development Co of Hebrew University of Jerusalem, Futuragene Israel Ltd filed Critical Yissum Research Development Co of Hebrew University of Jerusalem
Publication of EP2405009A2 publication Critical patent/EP2405009A2/en
Publication of EP2405009A3 publication Critical patent/EP2405009A3/en
Application granted granted Critical
Publication of EP2405009B1 publication Critical patent/EP2405009B1/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C12N15/8246Non-starch polysaccharides, e.g. cellulose, fructans, levans
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0006Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1048Glycosyltransferases (2.4)
    • C12N9/1051Hexosyltransferases (2.4.1)
    • C12N9/1055Levansucrase (2.4.1.10)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
    • C12P7/08Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y101/00Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/99Oxidoreductases acting on the CH-OH group of donors (1.1) with other acceptors (1.1.99)
    • C12Y101/99018Cellobiose oxidase (1.1.99.18)
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Paper (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present invention relates to a transgenic plant expressing CDH. The invention furthermore relates to s method of making a bio-fuel, wood, paper, textile or yarn product, comprising: a) over-expressing a nucleic acid molecule encoding CDH in a plant; and b) processing the cellulose or hemicellulose portion of the plant into said product.
EP11003665.4A 2007-03-29 2008-03-26 Transgenic plants containing soluble cell wall polysaccharides Active EP2405009B1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US90734407P 2007-03-29 2007-03-29
EP08720044A EP2140009A2 (en) 2007-03-29 2008-03-26 Transgenic plants containing soluble cell wall polysaccharides

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
EP08720044A Division EP2140009A2 (en) 2007-03-29 2008-03-26 Transgenic plants containing soluble cell wall polysaccharides

Publications (3)

Publication Number Publication Date
EP2405009A2 EP2405009A2 (en) 2012-01-11
EP2405009A3 true EP2405009A3 (en) 2012-02-29
EP2405009B1 EP2405009B1 (en) 2016-08-03

Family

ID=39641392

Family Applications (2)

Application Number Title Priority Date Filing Date
EP08720044A Withdrawn EP2140009A2 (en) 2007-03-29 2008-03-26 Transgenic plants containing soluble cell wall polysaccharides
EP11003665.4A Active EP2405009B1 (en) 2007-03-29 2008-03-26 Transgenic plants containing soluble cell wall polysaccharides

Family Applications Before (1)

Application Number Title Priority Date Filing Date
EP08720044A Withdrawn EP2140009A2 (en) 2007-03-29 2008-03-26 Transgenic plants containing soluble cell wall polysaccharides

Country Status (10)

Country Link
US (1) US10011846B2 (en)
EP (2) EP2140009A2 (en)
CN (2) CN101796189A (en)
AR (1) AR065851A1 (en)
BR (1) BRPI0808598A8 (en)
CA (1) CA2681614C (en)
NZ (2) NZ580623A (en)
PT (1) PT2405009T (en)
WO (1) WO2008120194A2 (en)
ZA (1) ZA200907369B (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2681614C (en) 2007-03-29 2017-01-03 Cbd Technologies Ltd. Transgenic plants containing water soluble polysaccharides and comprising cellobiose dehydrogenase (cdh) in the cell wall
WO2008120193A1 (en) * 2007-03-29 2008-10-09 Cbd Technologies Ltd. Plants over-expressing pme
CA2787698C (en) * 2010-01-25 2018-07-31 Bayer Cropscience Nv Methods for manufacturing plant cell walls comprising chitin
CN109837787A (en) * 2019-01-18 2019-06-04 易艾通智慧科技有限公司 A kind of traditional Chinese medicine Chinese mugwort ginger paper and its preparation process
CN115606503B (en) * 2022-12-19 2023-03-31 北京花乡花木集团有限公司 Tissue culture method of aster

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000009729A2 (en) * 1998-08-14 2000-02-24 Pioneer Hi-Bred International, Inc. Expression of chitin synthase and chitin deacetylase genes in plants to alter the cell wall for industrial uses and improved disease resistance
US6033891A (en) * 1999-03-09 2000-03-07 Novo Nordisk Biotech, Inc. Nucleic acids encoding polypeptides having cellobiose dehydrogenase activity
EP1482033A1 (en) * 2002-02-25 2004-12-01 Oji Paper Co., Ltd. Cellulose digesting enzyme gene and utilization of the gene

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US212050A (en) * 1879-02-04 Improvement sn combination-ladders
US5525502A (en) 1991-10-09 1996-06-11 Institute For Molecular Biology & Biotechnology/Forth DNA encoding chitin deacetylase
DK87592D0 (en) 1992-07-03 1992-07-03 Novo Nordisk As ENZYME
CA2176109A1 (en) 1993-11-09 1995-05-18 Perry Girard Caimi Transgenic fructan accumulating crops and methods for their production
NL1002275C2 (en) * 1996-02-07 1997-08-08 Have D J Van Der Bv Modification of polysaccharides.
IL121404A0 (en) * 1997-07-27 1998-01-04 Yissum Res Dev Co Transgenic higher plants of altered structural morphology
DE69828193T2 (en) * 1997-10-31 2005-12-01 The Board Of Regents Of The University Of Oklahoma, Norman HYALURONAN SYNTHASE GENE AND ITS USE
JP3030431B2 (en) 1997-12-02 2000-04-10 農林水産省食品総合研究所長 Chitin deacetylase gene, vector containing the gene, and transformant
AU3204699A (en) * 1998-03-26 1999-10-18 E.I. Du Pont De Nemours And Company Plant 1,3-beta-d-glucan synthase and brittle-1 encoding sequences
CA2341078A1 (en) 1998-08-25 2000-03-02 Pioneer Hi-Bred International, Inc. Plant glutamine: fructose-6-phosphate amidotransferase nucleic acids
WO2002034926A2 (en) * 2000-10-20 2002-05-02 Michigan State University Transgenic plants containing ligninase and cellulase which degrade lignin and cellulose to fermentable sugars
DE10143205A1 (en) 2001-09-04 2003-03-20 Icon Genetics Ag Process for protein production in plants
DE602004030345D1 (en) 2003-07-31 2011-01-13 Toyo Boseki HYALURONIC ACID PRODUCING PLANT
CN104293912A (en) * 2003-12-16 2015-01-21 先锋高级育种国际公司 Dominant gene suppression transgenes and methods of using same
DE602005015473D1 (en) 2004-09-23 2009-08-27 Bayer Cropscience Ag METHOD AND MEANS FOR PRODUCING HYALURONANE
EP1640457A1 (en) * 2004-09-23 2006-03-29 Bayer CropScience GmbH Process and means for the production of hyaluronan
GB0422977D0 (en) 2004-10-15 2004-11-17 Swetree Technologies Ab Methods for improving tree growth and wood properties
MX2007008050A (en) 2005-01-06 2007-08-21 Novozymes Inc Polypeptides having cellobiohydrlase activity and polynucleotides encoding same.
DK1951878T3 (en) 2005-10-05 2015-05-26 Bayer Ip Gmbh Plants with increased hyaluronanproduktion
US8124842B2 (en) * 2005-10-05 2012-02-28 Bayer Cropscience Ag Plants having an increased content of amino sugars
EP1951878B1 (en) 2005-10-05 2015-02-25 Bayer Intellectual Property GmbH Plants with increased hyaluronan production
WO2008120193A1 (en) 2007-03-29 2008-10-09 Cbd Technologies Ltd. Plants over-expressing pme
CA2681614C (en) 2007-03-29 2017-01-03 Cbd Technologies Ltd. Transgenic plants containing water soluble polysaccharides and comprising cellobiose dehydrogenase (cdh) in the cell wall

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000009729A2 (en) * 1998-08-14 2000-02-24 Pioneer Hi-Bred International, Inc. Expression of chitin synthase and chitin deacetylase genes in plants to alter the cell wall for industrial uses and improved disease resistance
US6033891A (en) * 1999-03-09 2000-03-07 Novo Nordisk Biotech, Inc. Nucleic acids encoding polypeptides having cellobiose dehydrogenase activity
EP1482033A1 (en) * 2002-02-25 2004-12-01 Oji Paper Co., Ltd. Cellulose digesting enzyme gene and utilization of the gene

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
FARROKHI NASER ET AL: "Plant cell wall biosynthesis: genetic, biochemical and functional genomics approaches to the identification of key genes.", PLANT BIOTECHNOLOGY JOURNAL MAR 2006, vol. 4, no. 2, March 2006 (2006-03-01), pages 145 - 167, XP002491105, ISSN: 1467-7652 *

Also Published As

Publication number Publication date
US10011846B2 (en) 2018-07-03
WO2008120194A3 (en) 2009-01-08
US20100137568A1 (en) 2010-06-03
NZ593692A (en) 2012-08-31
CN101796189A (en) 2010-08-04
CA2681614A1 (en) 2008-10-09
EP2140009A2 (en) 2010-01-06
BRPI0808598A2 (en) 2015-07-28
EP2405009B1 (en) 2016-08-03
WO2008120194A2 (en) 2008-10-09
AR065851A1 (en) 2009-07-08
CA2681614C (en) 2017-01-03
EP2405009A2 (en) 2012-01-11
PT2405009T (en) 2016-11-07
ZA200907369B (en) 2010-12-29
NZ580623A (en) 2012-04-27
CN108192909A (en) 2018-06-22
BRPI0808598A8 (en) 2017-05-09

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