EP2355929A1 - Sample carrier and method for achieving comparable analytical results by aligning the substances on a uniform plane - Google Patents

Sample carrier and method for achieving comparable analytical results by aligning the substances on a uniform plane

Info

Publication number
EP2355929A1
EP2355929A1 EP09749018A EP09749018A EP2355929A1 EP 2355929 A1 EP2355929 A1 EP 2355929A1 EP 09749018 A EP09749018 A EP 09749018A EP 09749018 A EP09749018 A EP 09749018A EP 2355929 A1 EP2355929 A1 EP 2355929A1
Authority
EP
European Patent Office
Prior art keywords
sample carrier
substances
recesses
substance
vessel
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP09749018A
Other languages
German (de)
French (fr)
Other versions
EP2355929B1 (en
Inventor
Andreas Fischer
Dagmar Lischke
Dagmar Peters-Groth
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gruenenthal GmbH
Original Assignee
Gruenenthal GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gruenenthal GmbH filed Critical Gruenenthal GmbH
Priority to EP09749018.9A priority Critical patent/EP2355929B1/en
Publication of EP2355929A1 publication Critical patent/EP2355929A1/en
Application granted granted Critical
Publication of EP2355929B1 publication Critical patent/EP2355929B1/en
Not-in-force legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50853—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/06—Test-tube stands; Test-tube holders
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0642—Filling fluids into wells by specific techniques
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50855—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates using modular assemblies of strips or of individual wells

Definitions

  • the present invention relates to a sample carrier, for example a multiwell platform, with one or a plurality of recesses, in each of which a substance to be analyzed is located.
  • the present invention further relates to a method in which one or more substances, which are each located in a recess of a sample carrier, are aligned prior to their analysis on a uniform plane relative to the surface of the sample carrier.
  • the object is achieved with a sample carrier having a plurality of recesses, in each of which a substance to be analyzed is located, wherein it has a means with which the substances to be analyzed can be aligned respectively to the same plane relative to the sample carrier.
  • the sample carrier according to the invention is, for example, a so-called multiwell platform.
  • the sample carrier has one or any multiplicity of recesses, in particular bores, which are preferably arranged in a uniform pattern, very particularly preferably equidistantly are.
  • the recesses extend through the entire thickness of the sample carrier.
  • the recesses may have any cross-section, but are preferably circular.
  • the recesses are lined with a sleeve, in particular a sleeve, on which the least possible adhesion of the substances takes place. For example, it is a Teflon sleeve.
  • each recess for example, coated with a non-stick coating, and / or the entire sample carrier is made of an inert material, which particularly preferably also has non-stick properties.
  • a vessel is preferably arranged in each case displaceably along the recess.
  • the vessel may be part of a stamp, in particular be arranged on its upper side.
  • the stamp can be made of glass, metal, especially steel and / or plastic. He may also have a non-stick coating.
  • This vessel can be configured as desired. For example, it may be flat but also convex and / or concave.
  • the substance to be analyzed is located in the vessel and / or on the vessel.
  • the height of the substance preferably extends at least partially at least to the upper edge of the vessel, but is particularly preferably beyond.
  • the substance to be analyzed can each be placed on the vessel.
  • the production of the respective substance takes place in the vessel, so that the vessel can also be designed as a production vessel.
  • the preparation preferably takes place in a comparatively lowered state of the vessel within the sample carrier.
  • the vessel may be made of glass, metal, in particular steel, and / or plastic. It may also have a non-stick coating.
  • the sample carrier now has a means with which the substance to be analyzed can be aligned relative to the surface of the sample carrier, in each case on the same plane, in particular horizontal plane, preferably above the sample carrier.
  • This means is preferably a covering means covering one side of each recess.
  • the respective substance is moved along the recess in the direction of the cover before its analysis, in particular displaced, which, for example, with the above-described Stamp can be made, which is pressed from below against the vessel.
  • the substance to be analyzed in each case can also simply be filled into the respective recess, then it falls onto the cover and preferably remains at least partially attached thereto.
  • each substance and / or the respective vessel comes into contact with the covering means, so that these are then all in the same plane relative to the surface of the sample carrier. Accordingly, in the case of the sample carrier according to the invention, all substances have the same position, at least in some areas, relative to the surface of the sample carrier. As a result, the quality of the comparability of the analysis, for example, in the Röntengenpulverdiffraktometriefug in reflection is increased.
  • the covering means of the respective substance and / or the respective vessel serves as a stop. Preferably, these are pressed against the cover and particularly preferably pressed flat.
  • the covering means is preferably provided in one piece and therefore covers all ends of the existing recesses on one side of the sample carrier. Particularly preferably, the transmission of the covering agent is as high as possible, in particular with regard to X-rays.
  • the covering means is preferably a foil, in particular an X-ray-permeable foil.
  • a film according to the invention has a thickness in the micron range.
  • the covering means is a plate, in particular an X-ray transparent plate, which has a higher mechanical stability than a foil and in particular is not damaged or plastically deformed by the substances and / or the vessels.
  • the covering means can also be used to prevent cross-contamination of the substances with one another.
  • a spacer is arranged between the sample carrier and the cover.
  • the cover means in particular reversibly, is then preferably arranged on this spacer.
  • the covering means in particular if it is a film, adhered to the spacer.
  • the spacer has recesses, for example in the form of through holes, which are coaxial with the recesses of the Sample carrier are arranged.
  • the thickness of this spacer is greater than the amount by which the sleeves, which are arranged in the recesses of the sample holder, protrude from the surface of the sample carrier. As a rule, a thickness of a few millimeters is sufficient.
  • the area of the recess in the spacer is equal to or greater than the area of the underlying recess in the sample carrier.
  • the area of each recess in the spacer is so large that it can receive the outer edge of the sleeves which are located in the recesses in the sample carrier.
  • the spacer is not located on the edge of a sleeve but directly on the respective surface of the sample carrier. Now, if the substances are moved exactly to the outer edge of the spacer, where the covering is as a stop, they are all aligned exactly in the same plane relative to the surface of the sample carrier. For example, with a horizontally oriented sample carrier, they are located at exactly the same height above it.
  • a locking means is arranged on the side facing away from the sample carrier of the cover.
  • This counter-agent increases in particular the mechanical strength of the cover and is particularly advantageous if the cover is a film.
  • the securing means is preferably fastened to the sample carrier such that the covering means is located between the securing means and the sample carrier.
  • the locking means it is ensured that the substances and / or the vessels do not damage the covering means when they are brought into contact with it.
  • the counter-agent the substances can be pressed against the covering means with comparatively high force, without the covering means changing its position and / or being mechanically destroyed.
  • the counter-agent can also serve as a stop for the substances and / or the vessels. For the actual analysis, the counter-agent is then preferably removed from the sample carrier.
  • the sample carrier according to the invention is suitable for any analysis, but in particular for high-throughput screening and in particular for the analysis of reflection measurements by means of X-ray powder diffractometry.
  • Another object of the present invention is a method for analyzing a plurality of substances, each located in the recess of a sample carrier, in which the substances are aligned prior to their analysis on a uniform plane relative to the sample carrier.
  • Alignment may be by manual or mechanical force and / or gravity.
  • sample carrier according to the invention applies equally to the method according to the invention and vice versa.
  • the method according to the invention can be carried out in particular with the sample carrier according to the invention.
  • the recesses are covered with a covering.
  • This covering means serves all samples and / or the vessel, on or in which they are located, preferably as a stop, but also to avoid cross-contamination of the substances with each other.
  • the substances are brought to the cover means to the plant, preferably pressed against it.
  • the substances to be analyzed are in each case in and / or on a vessel which is arranged longitudinally displaceable in each case in a recess of the sample carrier, these vessels are moved in the direction of the cover before the analysis of the substances until the substance and / or the vessel touches the cover in each case.
  • FIGS 1- 1-5 These explanations are merely exemplary and do not limit the general inventive concept. The explanations apply equally to both subjects of the invention.
  • FIG. 1 shows a sample carrier, here a multiwell platform.
  • FIG. 2 shows a stamp with a vessel.
  • FIG. 3 shows the spacer
  • FIG. 4 shows the counter plate.
  • FIG. 5 shows the sample carrier before the measurement.
  • FIG. 1 shows the sample carrier 1 according to the invention in two views.
  • This is a multiwell plate which has a multiplicity, here 96, of through holes 2, which therefore extend over the entire thickness D of the sample carrier.
  • the holes 2 are arranged equidistantly in a uniform pattern.
  • Each hole is lined with a Teflon sleeve 8, which protrudes by a certain amount over the surface 1.1 of the sample carrier, this amount varies, usually max. Does not exceed 2 mm.
  • the Teflon sleeve has an outer diameter 8.1.
  • a punch 9 is arranged, which is shown in Figure 2.
  • the stamp is made of steel and / or Teflon and has at its upper end a preferably flat glass plate 3, the vessel on which the substance to be analyzed 4 arranged and / or on which the substance to be analyzed is prepared.
  • the diameter of the punch 9 and the vessel 3 is dimensioned so that the punch can be moved with moderate effort within the sleeve.
  • Figure 3 shows the spacer 5, which also has a plurality of through holes 5.1, whose arrangement corresponds to the arrangement of the holes 2 in the sample carrier, so that the center of the holes 2, 5.1 is aligned when the spacer 5 is placed on the sample carrier 1.
  • the diameter 5.2 of the holes 5.1 is chosen so that it is at least equal, preferably slightly larger than the outer diameter of the sleeves 8, so that the upper edge of the recesses 5.1 can be added.
  • the spacer 5 can be connected by four screws in the corner with the sample carrier. After the spacer 5 has been placed on the sample carrier 1, the edge of any sleeve is beyond the surface of the spacer.
  • a glue a water-soluble adhesive has proven itself.
  • the surface of the spacer 5 is at least partially coated with the adhesive and then the film 6 glued.
  • the counter plate 7 which is shown in Figure 4, attached to the glued-with-foil spacer, for example screwed.
  • the analysis of the substances is carried out as follows. In each case a substance 4 is placed on the vessel 3 of a sample carrier or produced therein. The vessels 3 and the punches 9 are in a comparatively lowered position. Thereafter, or before the adhered with a film 6 spacer 5 is mounted on the surface 1.1 of the sample carrier 1. Then the counter plate is attached. Thereafter, the sample carrier is rotated together with spacer 5, slide 6 and counter plate 7 by 180 °, so that the counter plate points down. Subsequently, the punches 9 and thus the vessels 3 are manually or mechanically displaced in the direction of the covering means 6, until the edge 3.1 of the vessels 3 rests against the covering means 6, respectively.
  • the counter plate 7 prevents the film 6 is damaged by the vessels 3 in particular overstretched. But the counter plate also serves as a stop for the vessels / stamp, so that the edge 3.1 of all vessels and the position of the substances 4 after moving the stamp has exactly the same position relative to the surface of the sample carrier 1, i. the substances 4 and / or the edge 3.1 then all have the same distance from the surface 1.1 of the sample carrier.
  • the substances 4 can partially stick to the film 6. Thereafter, the sample carrier is rotated back by 180 ° and now the counter plate 7 removed from the sample carrier 1 and the sample carrier preferably horizontally mounted on an analyzer and carried out the substance by substance analysis.
  • the substances all have the same height, which is advantageous for the comparability of the measurements.
  • the covering means itself has sufficient mechanical stability that it is not damaged or plastically deformed by the substances and / or the vessels.
  • the sample carrier provided with the covering means only, as described above, aligned with the covering means down and each substance to be analyzed are each filled into a bore, which then falls on the cover and clinging there. Then the sample carrier can be turned back and the analysis carried out.
  • FIG. 5 shows the sample carrier after the alignment. It can be clearly seen how the substances on the film 6, which is glued to the spacer 5 adheres. All substances are at the same height.
  • reaction vessel 3 vessel, reaction vessel, glass plate 3.1 upper edge of the reaction vessel

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

The present invention relates to a sample carrier, for example, a multiwell platform having one or a plurality of recesses, in each of which there is a substance to be analyzed. The present invention also relates to a method in which one or more substances, which are in a recess in a sample carrier, are aligned on a uniform plane relative to the surface of the sample carrier before being analyzed.

Description

Probenträger und Verfahren zur Erzielung vergleichbarer Analyseergebnisse durch Ausrichtung der Substanzen auf eine einheitliche Ebene Sample carriers and methods for achieving comparable analysis results by aligning the substances to a uniform level
Die vorliegende Erfindung betrifft einen Probenträger, beispielsweise eine Multiwellplattform, mit einer oder einer Vielzahl von Ausnehmungen, in denen sich jeweils eine zu analysierende Substanz befindet. Die vorliegende Erfindung betrifft des weiteren ein Verfahren, bei dem eine oder mehrere Substanzen, die sich jeweils in einer Ausnehmung eines Probenträgers befinden, vor deren Analyse auf eine einheitliche Ebene relativ zur Oberfläche des Probenträgers ausgerichtet werden.The present invention relates to a sample carrier, for example a multiwell platform, with one or a plurality of recesses, in each of which a substance to be analyzed is located. The present invention further relates to a method in which one or more substances, which are each located in a recess of a sample carrier, are aligned prior to their analysis on a uniform plane relative to the surface of the sample carrier.
Untersuchungen von Substanzen im Hochdurchsatzverfahren machen es notwendig, Reaktionen und/oder analytische Messungen in Probenträgern durchzuführen, die eine Vielzahl von Ausnehmungen aufweisen, in oder auf denen sich die jeweilige Substanz befindet. Ein Analyseverfahren ist dabei z.B. die Röntengenpulverdiffraktometrie, die bei der Bestimmung von Polymorphismus ein Standardverfahren ist. Bei dieser Messung wird nacheinander ein Röntgenstrahl jeweils auf eine Substanz auf dem Probenträger gerichtet und der davon reflektierte Anteil ausgewertet. Die Analyse von Substanzen in derartigen Probenträgern hat jedoch den Nachteil, dass die dabei erzielten Ergebnisse nur bedingt miteinander vergleichbar sind.Examination of substances in high-throughput procedures makes it necessary to carry out reactions and / or analytical measurements in sample carriers which have a multiplicity of recesses in or on which the respective substance is located. An analysis method is e.g. X-ray powder diffraction, which is a standard procedure in the determination of polymorphism. In this measurement, an X-ray beam is successively directed to a substance on the sample carrier and the portion reflected therefrom is evaluated. However, the analysis of substances in such sample carriers has the disadvantage that the results obtained are only partially comparable with each other.
Es war deshalb die Aufgabe der vorliegenden Erfindung einen Probenträger und ein Analyseverfahren zur Verfügung zu stellen, mit dem Analysen der auf oder in dem Probenträger befindlichen Substanzen durchführbar sind, die eine sehr gute Vergleichbarkeit der erzielten Resultate gewährleistet.It was therefore the object of the present invention to provide a sample carrier and an analysis method with which analyzes of the substances located on or in the sample carrier can be carried out, which ensures a very good comparability of the results obtained.
Gelöst wird die Aufgabe mit einem Probenträger mit einer Vielzahl von Ausnehmungen, in denen sich jeweils eine zu analysierendes Substanz befindet, wobei er ein Mittel aufweist, mit dem die zu analysierenden Substanzen jeweils auf dieselbe Ebene relativ zu dem Probenträger ausrichtbar sind.The object is achieved with a sample carrier having a plurality of recesses, in each of which a substance to be analyzed is located, wherein it has a means with which the substances to be analyzed can be aligned respectively to the same plane relative to the sample carrier.
Bei dem erfindungsgemäßen Probenträger handelt es sich beispielsweise um eine sogenannte Multiwellplattform. Der Probenträger weist eine oder eine beliebige Vielzahl von Ausnehmungen, insbesondere Bohrungen, auf, die vorzugsweise in einem gleichmäßigen Muster, ganz besonders bevorzugt äquidistant, angeordnet sind. Vorzugsweise erstrecken sich die Ausnehmungen durch die gesamte Dicke des Probenträgers. Die Ausnehmungen können einen beliebigen Querschnitt aufweisen, sind jedoch vorzugsweise kreisrund. Vorzugsweise sind die Ausnehmungen mit einer Hülse, insbesondere einer Hülse, an der eine möglichst geringe Anhaftung der Substanzen stattfindet, ausgekleidet. Beispielsweise handelt es sich um eine Teflonhülse. In einer anderen bevorzugten Ausführungsform ist jede Ausnehmung, beispielsweise mit einer Antihaftbeschichtung, beschichtet und/oder der gesamte Probenträger ist aus einem inerten Material, das besonders bevorzugt auch Antihafteigenschaften aufweist.The sample carrier according to the invention is, for example, a so-called multiwell platform. The sample carrier has one or any multiplicity of recesses, in particular bores, which are preferably arranged in a uniform pattern, very particularly preferably equidistantly are. Preferably, the recesses extend through the entire thickness of the sample carrier. The recesses may have any cross-section, but are preferably circular. Preferably, the recesses are lined with a sleeve, in particular a sleeve, on which the least possible adhesion of the substances takes place. For example, it is a Teflon sleeve. In another preferred embodiment, each recess, for example, coated with a non-stick coating, and / or the entire sample carrier is made of an inert material, which particularly preferably also has non-stick properties.
In diesen Ausnehmungen ist vorzugsweise jeweils ein Gefäß verschieblich entlang der Ausnehmung angeordnet. Beispielsweise kann das Gefäß Teil eines Stempels sein, insbesondere an dessen Oberseite angeordnet sein. Der Stempel kann aus Glas, Metall, insbesondere Stahl und/oder Kunststoff gefertigt sein. Er kann ebenfalls eine Antihaftbeschichtung aufweisen. Dieses Gefäß kann beliebig ausgestaltet sein. Beispielsweise kann es plan aber auch konvex und/oder konkav gewölbt sein. In dem Gefäß und/oder auf dem Gefäß befindet sich jeweils die zu analysierende Substanz. Die Höhe der Substanz erstreckt sich vorzugsweise zumindest teilweise zumindest bis zum oberen Rand des Gefäßes, steht jedoch besonders bevorzugt darüber hinaus. Die zu analysierende Substanz kann jeweils auf das Gefäß gelegt werden. Vorzugsweise findet in dem Gefäß jedoch auch die Herstellung der jeweiligen Substanz, beispielsweise durch Reaktion und/oder eine verfahrenstechnische Operation, statt, so dass das Gefäß auch als Herstellungsgefäß ausgestaltet sein kann. Die Herstellung findet dabei vorzugsweise in einem vergleichsweise abgesenkten Zustand des Gefäßes innerhalb des Probenträgers statt. Das Gefäß kann aus Glas, Metall, insbesondere Stahl, und/oder Kunststoff gefertigt sein. Es kann ebenfalls eine Antihaftbeschichtung aufweisen.In these recesses, a vessel is preferably arranged in each case displaceably along the recess. For example, the vessel may be part of a stamp, in particular be arranged on its upper side. The stamp can be made of glass, metal, especially steel and / or plastic. He may also have a non-stick coating. This vessel can be configured as desired. For example, it may be flat but also convex and / or concave. In each case the substance to be analyzed is located in the vessel and / or on the vessel. The height of the substance preferably extends at least partially at least to the upper edge of the vessel, but is particularly preferably beyond. The substance to be analyzed can each be placed on the vessel. Preferably, however, the production of the respective substance, for example by reaction and / or a procedural operation, takes place in the vessel, so that the vessel can also be designed as a production vessel. The preparation preferably takes place in a comparatively lowered state of the vessel within the sample carrier. The vessel may be made of glass, metal, in particular steel, and / or plastic. It may also have a non-stick coating.
Erfindungsgemäß weist der Probenträger nun ein Mittel, auf, mit dem die zu analysierende Substanz jeweils auf dieselbe Ebene, insbesondere horizontale Ebene, vorzugsweise oberhalb des Probenträgers, relativ zu der Oberfläche des Probenträgers ausrichtbar sind. Dieses Mittel ist vorzugsweise ein Abdeckmittel, das eine Seite jeder Ausnehmungen abgedeckt. Vorzugsweise wird die jeweilige Substanz vor dessen Analyse entlang der Ausnehmung in Richtung der Abdeckung bewegt, insbesondere verschoben, was beispielsweise mit dem oben beschriebenen Stempel erfolgen kann, der von unten gegen das Gefäß gedrückt wird. Die jeweils zu analysierende Substanz kann aber auch einfach in die jeweilige Ausnehmung eingefüllt werden, fällt dann auf die Abdeckung und bleibt vorzugsweise zumindest teilweise daran hängen.According to the invention, the sample carrier now has a means with which the substance to be analyzed can be aligned relative to the surface of the sample carrier, in each case on the same plane, in particular horizontal plane, preferably above the sample carrier. This means is preferably a covering means covering one side of each recess. Preferably, the respective substance is moved along the recess in the direction of the cover before its analysis, in particular displaced, which, for example, with the above-described Stamp can be made, which is pressed from below against the vessel. However, the substance to be analyzed in each case can also simply be filled into the respective recess, then it falls onto the cover and preferably remains at least partially attached thereto.
So gelangt jede Substanz und/oder das jeweilige Gefäß in Kontakt mit dem Abdeckmittel, so dass sich diese anschließend alle in derselben Ebene relativ zu der Oberfläche des Probenträgers befinden. Bei dem erfindungsgemäßen Probenträger weisen demnach alle Substanzen zumindest in Teilbereichen bezogen auf die Oberfläche des Probenträgers dieselbe Lage auf. Dadurch wird die Qualität der Vergleichbarkeit der Analyse beispielsweise bei der Röntengenpulverdiffraktometriemessung in Reflektion erhöht.Thus, each substance and / or the respective vessel comes into contact with the covering means, so that these are then all in the same plane relative to the surface of the sample carrier. Accordingly, in the case of the sample carrier according to the invention, all substances have the same position, at least in some areas, relative to the surface of the sample carrier. As a result, the quality of the comparability of the analysis, for example, in the Röntengenpulverdiffraktometriemessung in reflection is increased.
Vorzugsweise dient das Abdeckmittel der jeweiligen Substanz und/oder dem jeweiligen Gefäß als Anschlag. Vorzugsweise werden diese gegen das Abdeckmittel gedrückt und besonders bevorzugt dabei flach gedrückt. Das Abdeckmittel ist vorzugsweise einstückig vorgesehen und deckt demnach alle Enden der vorhandenen Ausnehmungen auf einer Seite des Probenträgers ab. Besonders bevorzugt ist die Transmission des Abdeckmittels insbesondere in Bezug auf Röntgenstrahlen möglichst hoch. Vorzugsweise handelt es sich bei dem Abdeckmittel um eine Folie, insbesondere um eine Röntgenstrahl-durchlässige Folie. Eine Folie im Sinne der Erfindung weist eine Dicke im μm-Bereich auf. In einer anderen bevorzugten Ausführungsform ist das Abdeckmittel eine Platte, insbesondere eine Röntgenstrahl-durchlässige Platte, die eine höhere mechanische Stabilität aufweist als eine Folie und insbesondere von den Substanzen und/oder den Gefäßen nicht beschädigt oder plastisch deformiert wird.Preferably, the covering means of the respective substance and / or the respective vessel serves as a stop. Preferably, these are pressed against the cover and particularly preferably pressed flat. The covering means is preferably provided in one piece and therefore covers all ends of the existing recesses on one side of the sample carrier. Particularly preferably, the transmission of the covering agent is as high as possible, in particular with regard to X-rays. The covering means is preferably a foil, in particular an X-ray-permeable foil. A film according to the invention has a thickness in the micron range. In another preferred embodiment, the covering means is a plate, in particular an X-ray transparent plate, which has a higher mechanical stability than a foil and in particular is not damaged or plastically deformed by the substances and / or the vessels.
Mit dem Abdeckmittel kann auch eine Kreuzkontamination der Substanzen untereinander verhindert werden.The covering means can also be used to prevent cross-contamination of the substances with one another.
Vorzugsweise ist zwischen dem Probenträger und dem Abdeckmittel ein Abstandhalter angeordnet. Auf diesem Abstandhalter wird dann vorzugsweise das Abdeckmittel, insbesondere reversibel angeordnet. Besonders bevorzugt wird das Abdeckmittel, insbesondere wenn es sich um eine Folie handelt, auf den Abstandhalter geklebt. Der Abstandhalter weist Ausnehmungen, beispielsweise in Form von Durchgangsbohrungen, auf, die koaxial zu den Ausnehmungen des Probenträgers angeordnet sind. Vorzugsweise ist die Dicke dieses Abstandhalters größer als der Betrag, um den die Hülsen, die in den Ausnehmungen des Probenträgers angeordnet sind, aus der Oberfläche des Probenträgers herausstehen. In der Regel reicht eine Dicke von wenigen Millimetern. Vorzugsweise ist die Fläche der Ausnehmung in dem Abstandhalter gleich oder größer als die Fläche der darunter befindlichen Ausnehmung in dem Probenträger. Insbesondere ist die Fläche jeder Ausnehmung in dem Abstandhalter so groß, dass sie den Außenrand der Hülsen, die sich in den Ausnehmungen in dem Probenträger befinden, aufnehmen kann. Dadurch liegt der Abstandhalter nicht auf dem Rand einer Hülse sondern direkt auf der jeweiligen Oberfläche des Probenträgers auf. Werden nun die Substanzen genau bis zu dem äußern Rand des Abstandhalters bewegt werden, wo sich das Abdeckmittel als Anschlag befindet, sind sie alle genau in derselben Ebene relativ zu der Oberfläche des Probenträgers ausgerichtet. Bei einem horizontal ausgerichteten Probenträger befinden sie sich beispielsweise genau in derselben Höhe darüber.Preferably, a spacer is arranged between the sample carrier and the cover. The cover means, in particular reversibly, is then preferably arranged on this spacer. Particularly preferably, the covering means, in particular if it is a film, adhered to the spacer. The spacer has recesses, for example in the form of through holes, which are coaxial with the recesses of the Sample carrier are arranged. Preferably, the thickness of this spacer is greater than the amount by which the sleeves, which are arranged in the recesses of the sample holder, protrude from the surface of the sample carrier. As a rule, a thickness of a few millimeters is sufficient. Preferably, the area of the recess in the spacer is equal to or greater than the area of the underlying recess in the sample carrier. In particular, the area of each recess in the spacer is so large that it can receive the outer edge of the sleeves which are located in the recesses in the sample carrier. As a result, the spacer is not located on the edge of a sleeve but directly on the respective surface of the sample carrier. Now, if the substances are moved exactly to the outer edge of the spacer, where the covering is as a stop, they are all aligned exactly in the same plane relative to the surface of the sample carrier. For example, with a horizontally oriented sample carrier, they are located at exactly the same height above it.
Vorzugsweise ist auf der dem Probenträger abgewandten Seite des Abdeckmittels ein Kontermittel angeordnet. Dieses Kontermittel erhöht insbesondere die mechanische Belastbarkeit des Abdeckmittels und ist insbesondere dann von Vorteil, wenn das Abdeckmittel eine Folie ist. Das Kontermittel wird vorzugsweise so an dem Probenträger befestigt, dass sich das Abdeckmittel zwischen dem Kontermittel und dem Probenträger befindet. Durch das Kontermittel wird sichergestellt, dass die Substanzen und/oder die Gefäße das Abdeckmittel nicht beschädigen, wenn sie mit ihm in Kontakt gebracht werden. Durch das Kontermittel können die Substanzen mit vergleichsweise hoher Kraft gegen das Abdeckmittel gedrückt werden können, ohne dass das Abdeckmittel seine Lage verändert und/oder mechanisch zerstört wird. Das Kontermittel kann auch als Anschlag für die Substanzen und/oder die Gefäße dienen. Zur eigentlichen Analyse wird das Kontermittel dann vorzugsweise von dem Probenträger entfernt.Preferably, a locking means is arranged on the side facing away from the sample carrier of the cover. This counter-agent increases in particular the mechanical strength of the cover and is particularly advantageous if the cover is a film. The securing means is preferably fastened to the sample carrier such that the covering means is located between the securing means and the sample carrier. By means of the locking means it is ensured that the substances and / or the vessels do not damage the covering means when they are brought into contact with it. By means of the counter-agent, the substances can be pressed against the covering means with comparatively high force, without the covering means changing its position and / or being mechanically destroyed. The counter-agent can also serve as a stop for the substances and / or the vessels. For the actual analysis, the counter-agent is then preferably removed from the sample carrier.
Der erfindungsgemäße Probenträger eignet sich für jede Analyse, insbesondere jedoch zum Hochdurchsatzscreening und hier insbesondere zur Analyse von Reflektionsmessungen mittels der Röntengenpulverdiffraktometrie. Ein weiter Gegenstand der vorliegenden Erfindung ist ein Verfahren zur Analyse einer Vielzahl von Substanzen, die sich jeweils in der Ausnehmung eines Probenträgers befinden, bei dem die Substanzen vor deren Analyse auf eine einheitliche Ebene relativ zu dem Probenträger ausgerichtet werden.The sample carrier according to the invention is suitable for any analysis, but in particular for high-throughput screening and in particular for the analysis of reflection measurements by means of X-ray powder diffractometry. Another object of the present invention is a method for analyzing a plurality of substances, each located in the recess of a sample carrier, in which the substances are aligned prior to their analysis on a uniform plane relative to the sample carrier.
Die Ausrichtung kann durch eine manuelle oder maschinelle Krafteinwirkung und/oder unter Einwirkung der Schwerkraft erfolgen.Alignment may be by manual or mechanical force and / or gravity.
Die zu dem erfindungsgemäßen Probenträger gemachten Ausführungen gelten für das erfindungsgemäße Verfahren gleichermaßen und umgekehrt. Das erfindungsgemäße Verfahren lässt sich insbesondere mit dem erfindungsgemäßen Probenträger durchführen.The statements made on the sample carrier according to the invention apply equally to the method according to the invention and vice versa. The method according to the invention can be carried out in particular with the sample carrier according to the invention.
Vorzugsweise werden die Ausnehmungen mit einem Abdeckmittel abgedeckt. Dieses Abdeckmittel dient allen Proben und/oder dem Gefäß, auf oder in dem sie sich befinden, vorzugsweise als Anschlag, aber auch um eine Kreuzkontamination der Substanzen untereinander zu vermeiden.Preferably, the recesses are covered with a covering. This covering means serves all samples and / or the vessel, on or in which they are located, preferably as a stop, but also to avoid cross-contamination of the substances with each other.
Vorzugsweise werden die Substanzen an das Abdeckmittel zur Anlage gebracht, vorzugsweise dagegen gedrückt.Preferably, the substances are brought to the cover means to the plant, preferably pressed against it.
In einer bevorzugten Ausführungsform befinden sich die zu analysierenden Substanzen jeweils in und/oder auf einem Gefäß, das längsverschieblich jeweils in einer Ausnehmung des Probenträger angeordnet ist, wobei diese Gefäße vor der Analyse der Substanzen jeweils in Richtung des Abdeckmittels bewegt werden, bis die Substanz und/oder das Gefäß das Abdeckmittel jeweils berührt.In a preferred embodiment, the substances to be analyzed are in each case in and / or on a vessel which is arranged longitudinally displaceable in each case in a recess of the sample carrier, these vessels are moved in the direction of the cover before the analysis of the substances until the substance and / or the vessel touches the cover in each case.
Im Folgenden wird die Erfindung anhand der Figuren 1- 5 erläutert. Diese Erläuterungen sind lediglich beispielhaft und schränken den allgemeinen Erfindungsgedanken nicht ein. Die Erläuterungen gelten für beide Erfindungsgegenstände gleichermaßen.In the following the invention will be explained with reference to FIGS 1- 1-5. These explanations are merely exemplary and do not limit the general inventive concept. The explanations apply equally to both subjects of the invention.
Figur 1 zeigt einen Probenträger, hier eine Multiwellplattform.FIG. 1 shows a sample carrier, here a multiwell platform.
Figur 2 zeigt einen Stempel mit einem Gefäß.FIG. 2 shows a stamp with a vessel.
Figur 3 zeigt den Abstandhalter.FIG. 3 shows the spacer.
Figur 4 zeigt die Konterplatte. Figur 5 den Probenträger vor der Messung.FIG. 4 shows the counter plate. FIG. 5 shows the sample carrier before the measurement.
Figur 1 zeigt den erfindungsgemäßen Probenträger 1 in zwei Ansichten. Es handelt sich um hier um eine Multiwellplatte Dieser weist eine Vielzahl, hier 96, von Durchgangsbohrungen 2 auf, die sich also über die gesamte Dicke D des Probenträgers erstrecken. Die Bohrungen 2 sind in einem gleichmäßigen Muster äquidistant angeordnet. Jede Bohrung ist mit einer Teflonhülse 8 ausgekleidet, die um einen bestimmten Betrag über die Oberfläche 1.1 des Probenträgers hinaussteht, wobei dieser Betrag variiert, in der Regel max. 2 mm nicht überschreitet. An ihrem oberen Ende hat die Teflonhülse einen Außendurchmesser 8.1. In jeder dieser Teflonhülsen 8 ist ein Stempel 9 angeordnet, der in Figur 2 dargestellt ist. Der Stempel besteht aus Stahl und/oder Teflon und besitzt an seinem oberen Ende eine vorzugsweise plane Glasplatte 3, das Gefäß, auf der die zu analysierende Substanz 4 angeordnet und/oder auf dem die zu analysierende Substanz hergestellt wird. Der Durchmesser des Stempels 9 und des Gefäßes 3 ist so bemessen, dass der Stempel unter mäßigem Kraftaufwand innerhalb der Hülse verschoben werden kann.FIG. 1 shows the sample carrier 1 according to the invention in two views. This is a multiwell plate which has a multiplicity, here 96, of through holes 2, which therefore extend over the entire thickness D of the sample carrier. The holes 2 are arranged equidistantly in a uniform pattern. Each hole is lined with a Teflon sleeve 8, which protrudes by a certain amount over the surface 1.1 of the sample carrier, this amount varies, usually max. Does not exceed 2 mm. At its upper end, the Teflon sleeve has an outer diameter 8.1. In each of these Teflon sleeves 8, a punch 9 is arranged, which is shown in Figure 2. The stamp is made of steel and / or Teflon and has at its upper end a preferably flat glass plate 3, the vessel on which the substance to be analyzed 4 arranged and / or on which the substance to be analyzed is prepared. The diameter of the punch 9 and the vessel 3 is dimensioned so that the punch can be moved with moderate effort within the sleeve.
Figur 3 zeigt den Abstandhalter 5, der ebenfalls eine Vielzahl von Durchgangsbohrungen 5.1 aufweist, deren Anordnung der Anordnung der Bohrungen 2 in dem Probenträger entspricht, so dass der Mittelpunkt der Bohrungen 2, 5.1 fluchtet, wenn der Abstandhalter 5 auf den Probenträger 1 gelegt wird. Der Durchmesser 5.2 der Bohrungen 5.1 wird dabei so gewählt, dass er mindestens gleich, vorzugsweise etwas größer, ist als der Außendurchmesser der Hülsen 8, so dass deren oberer Rand von den Ausnehmungen 5.1 aufgenommen werden kann. Der Abstandhalter 5 kann mittels vier Schrauben im Eckbereich mit dem Probenträger verbunden werden. Nachdem der Abstandhalter 5 auf den Probenträger 1 aufgesetzt worden ist, steht der Rand keiner Hülse über die Oberfläche des Abstandhalters hinaus.Figure 3 shows the spacer 5, which also has a plurality of through holes 5.1, whose arrangement corresponds to the arrangement of the holes 2 in the sample carrier, so that the center of the holes 2, 5.1 is aligned when the spacer 5 is placed on the sample carrier 1. The diameter 5.2 of the holes 5.1 is chosen so that it is at least equal, preferably slightly larger than the outer diameter of the sleeves 8, so that the upper edge of the recesses 5.1 can be added. The spacer 5 can be connected by four screws in the corner with the sample carrier. After the spacer 5 has been placed on the sample carrier 1, the edge of any sleeve is beyond the surface of the spacer.
Auf diesen Abstandshalter 5 wird nun ein Abdeckmittel 6, hier eine Folie, vorzugsweise eine röntgenstrahldurchlässige Folie, beispielsweise die Folie mit dem Handelsnamen Ultraphan, geklebt. Als Kleber hat sich ein wasserlöslicher Kleber bewährt. Die Oberfläche des Abstandhalters 5 wird zumindest abschnittsweise mit dem Kleber bestrichen und dann die Folie 6 aufgeklebt. Danach wird die Konterplatte 7 die in Figur 4 dargestellt ist, auf den mit Folie beklebten Abstandhalter befestigt, beispielsweise geschraubt.In this spacer 5 is now a covering 6, here a film, preferably a X-ray permeable film, for example, the film with the trade name Ultraphan, glued. As a glue, a water-soluble adhesive has proven itself. The surface of the spacer 5 is at least partially coated with the adhesive and then the film 6 glued. Thereafter, the counter plate 7 which is shown in Figure 4, attached to the glued-with-foil spacer, for example screwed.
Die Analyse der Substanzen wird wie folgt durchgeführt. Jeweils eine Substanz 4 wird auf das Gefäß 3 eines Probenträgers platziert oder darin hergestellt. Die Gefäße 3 und die Stempel 9 befinden sich dabei in einer vergleichsweise abgesenkten Stellung. Danach oder vorher wird der mit einer Folie 6 beklebte Abstandhalter 5 auf der Oberfläche 1.1 des Probenträgers 1 befestigt. Darauf wird die Konterplatte angebracht. Danach wird der Probenträger gemeinsam mit Abstandhalter 5, Folie 6 und Konterplatte 7 um 180° gedreht, so dass die Konterplatte nach unten zeigt. Anschließend werden die Stempel 9 und damit die Gefäße 3 manuell oder maschinell in Richtung des Abdeckmittels 6 verschoben, bis der Rand 3.1 der Gefäße 3 jeweils an dem Abdeckmittel 6 anliegt. Die Konterplatte 7 verhindert, dass die Folie 6 durch die Gefäße 3 beschädigt insbesondere überdehnt wird. Die Konterplatte dient aber auch als Anschlag für die Gefäße/Stempel, so dass der Rand 3.1 aller Gefäße und die Lage der Substanzen 4 nach dem Verschieben der Stempel exakt dieselbe Lage relativ zu der Oberfläche des Probenträgers 1 hat, d.h. die Substanzen 4 und/oder der Rand 3.1 haben anschließend alle denselben Abstand von der Oberfläche 1.1 des Probenträgers. Die Substanzen 4 können teilweise an der Folie 6 kleben. Danach wird der Probenträger wieder um 180° zurückgedreht und nun die Konterplatte 7 von dem Probenträger 1 abgenommen und der Probenträger vorzugsweise waagerecht, auf einem Analysengerät befestigt und die Analyse Substanz für Substanz durchgeführt. Die Substanzen haben dabei alle dieselbe Höhe, was für die Vergleichbarkeit der Messungen von Vorteil ist.The analysis of the substances is carried out as follows. In each case a substance 4 is placed on the vessel 3 of a sample carrier or produced therein. The vessels 3 and the punches 9 are in a comparatively lowered position. Thereafter, or before the adhered with a film 6 spacer 5 is mounted on the surface 1.1 of the sample carrier 1. Then the counter plate is attached. Thereafter, the sample carrier is rotated together with spacer 5, slide 6 and counter plate 7 by 180 °, so that the counter plate points down. Subsequently, the punches 9 and thus the vessels 3 are manually or mechanically displaced in the direction of the covering means 6, until the edge 3.1 of the vessels 3 rests against the covering means 6, respectively. The counter plate 7 prevents the film 6 is damaged by the vessels 3 in particular overstretched. But the counter plate also serves as a stop for the vessels / stamp, so that the edge 3.1 of all vessels and the position of the substances 4 after moving the stamp has exactly the same position relative to the surface of the sample carrier 1, i. the substances 4 and / or the edge 3.1 then all have the same distance from the surface 1.1 of the sample carrier. The substances 4 can partially stick to the film 6. Thereafter, the sample carrier is rotated back by 180 ° and now the counter plate 7 removed from the sample carrier 1 and the sample carrier preferably horizontally mounted on an analyzer and carried out the substance by substance analysis. The substances all have the same height, which is advantageous for the comparability of the measurements.
Der Fachmann versteht, dass auf die Konterplatte 7 verzichtet werden kann, wenn beispielsweise das Abdeckmittel selbst eine so hinreichende mechanische Stabilität aufweist, dass es von den Substanzen und/oder den Gefäßen nicht beschädigt oder plastisch deformiert wird.The skilled person understands that it is possible to dispense with the counter plate 7 if, for example, the covering means itself has sufficient mechanical stability that it is not damaged or plastically deformed by the substances and / or the vessels.
Auf die Stempel/Gefäße kann verzichtet werden, wenn die jeweils zu analysierende Substanz an dem Abdeckmittel anhaftet. In diesem Fall muss der mit dem Abdeckmittel versehene Probenträger nur, wie oben beschrieben, mit dem Abdeckmittel nach unten ausgerichtet und die jeweils zu analysierende Substanz jeweils in eine Bohrung eingefüllt werden, die dann auf das Abdeckmittel fällt und dort anhaftet. Sodann kann der Probenträger wieder zurückgedreht und die Analyse durchgeführt werden.On the stamp / vessels can be dispensed with, if the substance to be analyzed in each case adheres to the covering. In this case, the sample carrier provided with the covering means only, as described above, aligned with the covering means down and each substance to be analyzed are each filled into a bore, which then falls on the cover and clinging there. Then the sample carrier can be turned back and the analysis carried out.
Figur 5 zeigt den Probenträger nach der Ausrichtung. Es ist deutlich zu erkennen, wie die Substanzen an der Folie 6, die auf den Abstandshalter 5 geklebt ist, haftet. Alle Substanzen befinden sich auf derselben Höhe. FIG. 5 shows the sample carrier after the alignment. It can be clearly seen how the substances on the film 6, which is glued to the spacer 5 adheres. All substances are at the same height.
Bezugszeichenliste:LIST OF REFERENCE NUMBERS
1 Probenträger, Multiwellplatte, Multiwellplattform 1.1 Oberfläche des Probenträgers1 sample carrier, multiwell plate, multiwell platform 1.1 Surface of the sample carrier
2 Ausnehmung, Bohrung2 recess, bore
3 Gefäß, Reaktionsgefäß, Glasplatte 3.1 oberer Rand des Reaktionsgefäßes3 vessel, reaction vessel, glass plate 3.1 upper edge of the reaction vessel
4 zu analysierende Substanz4 substance to be analyzed
5 Abstandhalter, Lochplatte5 spacers, perforated plate
5.1 Ausnehmungen im Abstandhalter, Durchgangsbohrung5.1 recesses in the spacer, through hole
5.2 Durchmesser, Fläche der Ausnehmung5.2 diameter, area of the recess
6 Abdeckmittel, Abdeckung, Folie6 Cover, cover, foil
7 Kontermittel, Konterplatte7 counter means, counter plate
8 Auskleidung, Hülse, Teflonhülse 8.1 Fläche, Durchmesser der Hülse8 lining, sleeve, Teflon sleeve 8.1 surface, diameter of the sleeve
9 Stempel9 stamps
D Dicke des Probenträgers D thickness of the sample carrier

Claims

Patentansprüche: claims:
1. Probenträger (1 ) mit einer Vielzahl von Ausnehmungen (2), in denen sich jeweils eine zu analysierende Substanz (4) befindet, dadurch gekennzeichnet, dass er ein Mittel (5, 6, 7), aufweist, mit dem die zu analysierenden Substanzen jeweils auf dieselbe Höhe relativ zu der Oberfläche (1.1 ) des Probenträgers ausrichtbar sind.1. sample carrier (1) having a plurality of recesses (2), in each of which there is a substance to be analyzed (4), characterized in that it comprises means (5, 6, 7), with which the to be analyzed Substances are each aligned at the same height relative to the surface (1.1) of the sample carrier.
2. Probenträger nach Anspruch 1 , dadurch gekennzeichnet, dass in den Ausnehmungen (2) jeweils ein Gefäß (3) verschiebbar angeordnet ist, auf dem sich die zu analysierende Substanz (4) befindet.2. Sample carrier according to claim 1, characterized in that in the recesses (2) in each case a vessel (3) is displaceably arranged, on which the substance to be analyzed (4) is located.
3. Probenträger nach Anspruch 1oder 2, dadurch gekennzeichnet, dass auf dem Probenträger (1 ) ein Abdeckmittel (6) angeordnet ist, das der Substanz (4) und/oder dem Gefäß (3) zumindest teilweise als Anschlag dient.3. Sample carrier according to claim 1 or 2, characterized in that on the sample carrier (1) a covering means (6) is arranged, which serves the substance (4) and / or the vessel (3) at least partially as a stop.
4. Probenträger nach einem der voranstehenden Ansprüche, dadurch gekennzeichnet, dass zwischen dem Probenträger (1 ) und dem Abdeckmittel (6) ein Abstandhalter (5) angeordnet ist.4. Sample carrier according to one of the preceding claims, characterized in that between the sample carrier (1) and the covering means (6), a spacer (5) is arranged.
5. Probenträger nach Anspruch 4, dadurch gekennzeichnet, dass der Abstandhalter (5) Ausnehmungen (5.1 ) aufweist, die koaxial zu den Ausnehmungen (2) angeordnet sind.5. Sample carrier according to claim 4, characterized in that the spacer (5) recesses (5.1), which are arranged coaxially to the recesses (2).
6. Probenträger nach Anspruch 5, dadurch gekennzeichnet, dass die Fläche (5.2) der Ausnehmung (5.1 ) gleich oder größer ist als die Fläche (3.2) der darunter befindlichen Ausnehmung in dem Probenträger.6. Sample carrier according to claim 5, characterized in that the surface (5.2) of the recess (5.1) is equal to or larger than the surface (3.2) of the underlying recess in the sample carrier.
7. Probenträger nach einem der voranstehenden Ansprüche, dadurch gekennzeichnet, dass der Durchmesser der Ausnehmungen (5.1 ) größer ist als der Durchmesser der darunter befindlichen Ausnehmung in dem Probenträger.7. Sample carrier according to one of the preceding claims, characterized in that the diameter of the recesses (5.1) is greater than the diameter of the underlying recess in the sample carrier.
8. Probenträger nach einem der voranstehenden Ansprüche, dadurch gekennzeichnet, dass das Abdeckmittel (6) eine Folie ist.8. Sample carrier according to one of the preceding claims, characterized in that the covering means (6) is a film.
9. Probenträger nach Anspruch 8, dadurch gekennzeichnet, dass die Folie eine Röntgenstrahlen durchlässige Folie ist. 9. Sample carrier according to claim 8, characterized in that the film is an X-ray permeable film.
10. Probenträger nach einem der voranstehenden Ansprüche, dadurch gekennzeichnet, dass auf der dem Probenträger abgewandten Seite des Abdeckmittels (6) ein Kontermittel (7) angeordnet ist.10. Sample carrier according to one of the preceding claims, characterized in that on the side facing away from the sample carrier of the cover means (6) a counter-means (7) is arranged.
11. Verfahren zur Analyse einer Vielzahl von Substanzen (4), die sich jeweils in der Ausnehmung (2) eines Probenträgers befinden, dadurch gekennzeichnet, dass die Substanzen (4) vor deren Analyse auf eine einheitliche Ebene relativ zu dem Probenträger ausgerichtet werden.11. A method for analyzing a plurality of substances (4), each located in the recess (2) of a sample carrier, characterized in that the substances (4) are aligned prior to their analysis to a uniform level relative to the sample carrier.
12. Verfahren nach Anspruch 11 , dadurch gekennzeichnet, dass die Ausnehmungen (2) mit einem Abdeckmittel (6) abgedeckt werden.12. The method according to claim 11, characterized in that the recesses (2) are covered with a covering means (6).
13. Verfahren nach Anspruch 12, dadurch gekennzeichnet, dass die Substanzen an das Abdeckmittel (6) zur Anlage gebracht, vorzugsweise dagegen gedrückt, werden.13. The method according to claim 12, characterized in that the substances to the cover (6) brought to bear, preferably pressed against it, are.
14. Verfahren nach einem der Ansprüche 12 oder 13, dadurch gekennzeichnet, dass, die Substanzen sich jeweils in oder auf einem Gefäß (3) befinden, das längsverschieblich in einem Probenträger angeordnet ist und dass diese Gefäße (3) vor der Analyse der Substanz (4) jeweils in Richtung eines Abdeckmittels (6) bewegt werden bis die Substanz (4) und/oder das Gefäß (3) das Abdeckmittel (6) berührt. 14. The method according to any one of claims 12 or 13, characterized in that, the substances are each in or on a vessel (3) which is arranged longitudinally displaceable in a sample carrier and that these vessels (3) before the analysis of the substance ( 4) are each moved in the direction of a covering means (6) until the substance (4) and / or the vessel (3) touches the covering means (6).
EP09749018.9A 2008-11-06 2009-11-05 Sample carrier and method for achieving comparable analysis results by arranging substances on a common level Not-in-force EP2355929B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP09749018.9A EP2355929B1 (en) 2008-11-06 2009-11-05 Sample carrier and method for achieving comparable analysis results by arranging substances on a common level

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP08019415 2008-11-06
EP09749018.9A EP2355929B1 (en) 2008-11-06 2009-11-05 Sample carrier and method for achieving comparable analysis results by arranging substances on a common level
PCT/EP2009/007914 WO2010051990A1 (en) 2008-11-06 2009-11-05 Sample carrier and method for achieving comparable analytical results by aligning the substances on a uniform plane

Publications (2)

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EP2355929A1 true EP2355929A1 (en) 2011-08-17
EP2355929B1 EP2355929B1 (en) 2016-12-21

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EP09749018.9A Not-in-force EP2355929B1 (en) 2008-11-06 2009-11-05 Sample carrier and method for achieving comparable analysis results by arranging substances on a common level

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US (1) US20110245106A1 (en)
EP (1) EP2355929B1 (en)
ES (1) ES2619831T3 (en)
WO (1) WO2010051990A1 (en)

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Publication number Priority date Publication date Assignee Title
US3987719A (en) * 1973-07-23 1976-10-26 Leonard Kian Cooking utensil
DE4128698A1 (en) * 1991-08-29 1993-03-04 Boehringer Mannheim Gmbh Immunological analysis system for a specific combination reaction - contains extractors for specimen and reaction vessels on single rotor with washing and pipette stations on circumference
US6258325B1 (en) * 1993-04-19 2001-07-10 Ashok Ramesh Sanadi Method and apparatus for preventing cross-contamination of multi-well test plates
US5342581A (en) * 1993-04-19 1994-08-30 Sanadi Ashok R Apparatus for preventing cross-contamination of multi-well test plates
JP3032159B2 (en) * 1996-09-24 2000-04-10 株式会社日立製作所 Analysis system
US6241949B1 (en) * 1999-08-17 2001-06-05 Spectrumedix Corporation Spill-resistant microtitre trays and method of making
US6677162B1 (en) * 2000-07-18 2004-01-13 Uop Llc Process of parallel sample preparation
US20030085952A1 (en) * 2001-11-05 2003-05-08 Williams Roger O Apparatus and method for controlling the free surface of liquid in a well plate
ATE372168T1 (en) * 2004-07-06 2007-09-15 Hoffmann La Roche STAND SYSTEM WITH ADAPTER
US20070116613A1 (en) * 2005-11-23 2007-05-24 Donat Elsener Sample tube and system for storing and providing nucleic acid samples

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Title
See references of WO2010051990A1 *

Also Published As

Publication number Publication date
ES2619831T3 (en) 2017-06-27
WO2010051990A1 (en) 2010-05-14
EP2355929B1 (en) 2016-12-21
US20110245106A1 (en) 2011-10-06

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