EP2331691A4 - Engineered light-emitting reporter genes and medthods of use - Google Patents

Engineered light-emitting reporter genes and medthods of use

Info

Publication number
EP2331691A4
EP2331691A4 EP09811872A EP09811872A EP2331691A4 EP 2331691 A4 EP2331691 A4 EP 2331691A4 EP 09811872 A EP09811872 A EP 09811872A EP 09811872 A EP09811872 A EP 09811872A EP 2331691 A4 EP2331691 A4 EP 2331691A4
Authority
EP
European Patent Office
Prior art keywords
medthods
reporter genes
engineered light
emitting reporter
emitting
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP09811872A
Other languages
German (de)
French (fr)
Other versions
EP2331691A1 (en
Inventor
Stacy M Burns-Guydlsh
Pamela Reilly Contag
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
COBALT TECHNOLOGIES Inc
Original Assignee
COBALT TECHNOLOGIES Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US12/205,845 external-priority patent/US20090081715A1/en
Application filed by COBALT TECHNOLOGIES Inc filed Critical COBALT TECHNOLOGIES Inc
Priority claimed from PCT/US2009/036868 external-priority patent/WO2010027531A1/en
Publication of EP2331691A1 publication Critical patent/EP2331691A1/en
Publication of EP2331691A4 publication Critical patent/EP2331691A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Ecology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
EP09811872A 2008-09-05 2009-03-11 Engineered light-emitting reporter genes and medthods of use Withdrawn EP2331691A4 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12/205,845 US20090081715A1 (en) 2007-09-07 2008-09-05 Engineered Light-Emitting Reporter Genes
PCT/US2008/075515 WO2009033114A2 (en) 2007-09-07 2008-09-06 Engineered light-emitting reporter genes
PCT/US2009/036868 WO2010027531A1 (en) 2008-09-05 2009-03-11 Engineered light-emitting reporter genes and medthods of use

Publications (2)

Publication Number Publication Date
EP2331691A1 EP2331691A1 (en) 2011-06-15
EP2331691A4 true EP2331691A4 (en) 2012-04-18

Family

ID=41800459

Family Applications (1)

Application Number Title Priority Date Filing Date
EP09811872A Withdrawn EP2331691A4 (en) 2008-09-05 2009-03-11 Engineered light-emitting reporter genes and medthods of use

Country Status (1)

Country Link
EP (1) EP2331691A4 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6737245B1 (en) * 1999-09-08 2004-05-18 Xenogen Corporation Luciferase expression cassettes and methods of use
WO2008082726A2 (en) * 2006-09-18 2008-07-10 Cobalt Technologies, Inc. Real time monitoring of microbial enzymatic pathways
WO2009033114A2 (en) * 2007-09-07 2009-03-12 Cobalt Technologies, Inc. Engineered light-emitting reporter genes

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6737245B1 (en) * 1999-09-08 2004-05-18 Xenogen Corporation Luciferase expression cassettes and methods of use
WO2008082726A2 (en) * 2006-09-18 2008-07-10 Cobalt Technologies, Inc. Real time monitoring of microbial enzymatic pathways
WO2009033114A2 (en) * 2007-09-07 2009-03-12 Cobalt Technologies, Inc. Engineered light-emitting reporter genes

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
FEUSTEL LOTHAR ET AL: "Characterization and development of two reporter gene systems for Clostridium acetobutylicum", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 70, no. 2, 1 February 2004 (2004-02-01), pages 798 - 803, XP002601727, ISSN: 0099-2240, DOI: 10.1128/AEM.70.2.798-803.2004 *
ROGERS ET AL: "Clostridium acetobutylicum Mutants That Produce Butyraldehyde and Altered Quantities of Solvents.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 53, no. 12, 1 December 1987 (1987-12-01), pages 2761 - 2766, XP055021139, ISSN: 0099-2240 *
See also references of WO2010027531A1 *

Also Published As

Publication number Publication date
EP2331691A1 (en) 2011-06-15

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Legal Events

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AX Request for extension of the european patent

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DAX Request for extension of the european patent (deleted)
A4 Supplementary search report drawn up and despatched

Effective date: 20120316

RIC1 Information provided on ipc code assigned before grant

Ipc: C12R 1/145 20060101ALI20120312BHEP

Ipc: C12N 1/21 20060101ALI20120312BHEP

Ipc: C12N 15/63 20060101ALI20120312BHEP

Ipc: C12N 15/31 20060101AFI20120312BHEP

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Effective date: 20111016