EP2318527A4 - Methods for gene targeting - Google Patents

Methods for gene targeting

Info

Publication number
EP2318527A4
EP2318527A4 EP08782548A EP08782548A EP2318527A4 EP 2318527 A4 EP2318527 A4 EP 2318527A4 EP 08782548 A EP08782548 A EP 08782548A EP 08782548 A EP08782548 A EP 08782548A EP 2318527 A4 EP2318527 A4 EP 2318527A4
Authority
EP
European Patent Office
Prior art keywords
methods
gene targeting
targeting
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP08782548A
Other languages
German (de)
French (fr)
Other versions
EP2318527A1 (en
Inventor
Michael Strathmann
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of EP2318527A1 publication Critical patent/EP2318527A1/en
Publication of EP2318527A4 publication Critical patent/EP2318527A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1077—Pentosyltransferases (2.4.2)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/50—Fibroblast growth factors [FGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1051—Gene trapping, e.g. exon-, intron-, IRES-, signal sequence-trap cloning, trap vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
EP08782548A 2008-07-30 2008-07-30 Methods for gene targeting Withdrawn EP2318527A4 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2008/071667 WO2010014090A1 (en) 2008-07-30 2008-07-30 Methods for gene targeting

Publications (2)

Publication Number Publication Date
EP2318527A1 EP2318527A1 (en) 2011-05-11
EP2318527A4 true EP2318527A4 (en) 2012-07-04

Family

ID=41610616

Family Applications (1)

Application Number Title Priority Date Filing Date
EP08782548A Withdrawn EP2318527A4 (en) 2008-07-30 2008-07-30 Methods for gene targeting

Country Status (3)

Country Link
EP (1) EP2318527A4 (en)
CA (1) CA2732498A1 (en)
WO (1) WO2010014090A1 (en)

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999050426A1 (en) * 1998-03-27 1999-10-07 Lexicon Genetics Incorporated Vectors for gene mutagenesis and gene discovery
WO2001002555A1 (en) * 1999-07-06 2001-01-11 Institut Pasteur Method of making and identifying attenuated microorganisms, compositions utilizing the sequences responsible for attenuation, and preparations containing attenuated microorganisms
WO2002079467A2 (en) * 2001-03-29 2002-10-10 Københavns Universitet Antibiotic-free bacterial strain selection with antisense molecules
US6689610B1 (en) * 1989-08-22 2004-02-10 University Of Utah Research Foundation Cells and non-human organisms containing predetermined genomic modifications and positive-negative selection methods and vectors for making same
EP1607482A1 (en) * 2004-06-16 2005-12-21 Gbf-Gesellschaft Für Biotechnologische Forschung Mbh RNAi-based methods for selection of transfected eucaryotic cells
WO2007098427A2 (en) * 2006-02-18 2007-08-30 Michael Strathmann Massively multiplexed sequencing
DE102006016365A1 (en) * 2006-04-05 2007-10-11 Qiagen Gmbh Composition for inhibiting expression of one or more target genes in a eukaryotic cell comprises one or more genetic constructs comprising a target gene and a small interfering RNA tag
WO2008122314A1 (en) * 2007-04-10 2008-10-16 Qiagen Gmbh Rna interference tags
WO2010091837A1 (en) * 2009-02-11 2010-08-19 Qiagen Gmbh Rnai based selection system

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6480791B1 (en) * 1998-10-28 2002-11-12 Michael P. Strathmann Parallel methods for genomic analysis
US20080188001A1 (en) * 2007-02-06 2008-08-07 Michael Paul Strathmann Methods For Gene Targeting

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6689610B1 (en) * 1989-08-22 2004-02-10 University Of Utah Research Foundation Cells and non-human organisms containing predetermined genomic modifications and positive-negative selection methods and vectors for making same
WO1999050426A1 (en) * 1998-03-27 1999-10-07 Lexicon Genetics Incorporated Vectors for gene mutagenesis and gene discovery
WO2001002555A1 (en) * 1999-07-06 2001-01-11 Institut Pasteur Method of making and identifying attenuated microorganisms, compositions utilizing the sequences responsible for attenuation, and preparations containing attenuated microorganisms
WO2002079467A2 (en) * 2001-03-29 2002-10-10 Københavns Universitet Antibiotic-free bacterial strain selection with antisense molecules
EP1607482A1 (en) * 2004-06-16 2005-12-21 Gbf-Gesellschaft Für Biotechnologische Forschung Mbh RNAi-based methods for selection of transfected eucaryotic cells
WO2007098427A2 (en) * 2006-02-18 2007-08-30 Michael Strathmann Massively multiplexed sequencing
DE102006016365A1 (en) * 2006-04-05 2007-10-11 Qiagen Gmbh Composition for inhibiting expression of one or more target genes in a eukaryotic cell comprises one or more genetic constructs comprising a target gene and a small interfering RNA tag
WO2008122314A1 (en) * 2007-04-10 2008-10-16 Qiagen Gmbh Rna interference tags
WO2010091837A1 (en) * 2009-02-11 2010-08-19 Qiagen Gmbh Rnai based selection system

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
HAHN ET AL: "An siRNA-based system for differential regulation of ectopic gene expression constructs", JOURNAL OF BIOTECHNOLOGY, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 128, no. 4, 6 March 2007 (2007-03-06), pages 762 - 769, XP005916237, ISSN: 0168-1656, DOI: 10.1016/J.JBIOTEC.2006.12.015 *
KIM SUWON ET AL: "A screen for genes that suppress loss of contact inhibition: Identification of ING4 as a candidate tumor suppressor gene in human cancer", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, US, vol. 101, no. 46, 16 November 2004 (2004-11-16), pages 16251 - 16256, XP002501381, ISSN: 0027-8424, DOI: 10.1073/PNAS.0407158101 *
See also references of WO2010014090A1 *

Also Published As

Publication number Publication date
CA2732498A1 (en) 2010-02-04
EP2318527A1 (en) 2011-05-11
WO2010014090A1 (en) 2010-02-04

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