EP2313519A1 - Procédé de diagnostic/pronostic in vitro et kit pour évaluer la tolérance de la transplantation du foie - Google Patents

Procédé de diagnostic/pronostic in vitro et kit pour évaluer la tolérance de la transplantation du foie

Info

Publication number
EP2313519A1
EP2313519A1 EP09772548A EP09772548A EP2313519A1 EP 2313519 A1 EP2313519 A1 EP 2313519A1 EP 09772548 A EP09772548 A EP 09772548A EP 09772548 A EP09772548 A EP 09772548A EP 2313519 A1 EP2313519 A1 EP 2313519A1
Authority
EP
European Patent Office
Prior art keywords
genes
tol
tolerance
microarray
tolerant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP09772548A
Other languages
German (de)
English (en)
Inventor
Alberto Sanchez Fueyo
Juan José LOZANO SALVATELLA
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut dInvestigacions Biomediques August Pi i Sunyer IDIBAPS
Original Assignee
Institut dInvestigacions Biomediques August Pi i Sunyer IDIBAPS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/EP2008/058592 external-priority patent/WO2010000320A1/fr
Application filed by Institut dInvestigacions Biomediques August Pi i Sunyer IDIBAPS filed Critical Institut dInvestigacions Biomediques August Pi i Sunyer IDIBAPS
Priority to EP09772548A priority Critical patent/EP2313519A1/fr
Publication of EP2313519A1 publication Critical patent/EP2313519A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/24—Immunology or allergic disorders
    • G01N2800/245—Transplantation related diseases, e.g. graft versus host disease
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/52—Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne un procédé de diagnostic/pronostic in vitro et un kit, pour évaluer la tolérance de la transplantation du foie. La présente invention se rapporte à l'étude de motifs de la transcription du sang périphérique provenant de 80 bénéficiaires d’une transplantation du foie et de 16 individus sains non transplantés utilisant des matrices oligonucléotidiques et/ou une PCR quantitative en temps réel pour concevoir un test moléculaire applicable cliniquement. Ceci a résulté en la découverte et la validation de plusieurs signatures génétiques comprenant un petit nombre de gènes capables d'identifier des bénéficiaires tolérants et non tolérants avec une grande précision. Les marqueurs génétiques sont les KLRF1, SLAMF7, NKG7, IL2RB, KLRB1, FANCG, GNPTAB, CLIC3, PSMD14, ALG8, CX3CR1, RGS 3. De multiples sous ensembles de lymphocytes du sang périphérique contribuent à des motifs de la transcription associés à la tolérance avec des lymphocytes T NK et γdelta exerçant une influence prédominante. L'invention conclut que le profilage de la transcription du sang périphérique peut être utilisé pour identifier des bénéficiaires de transplantation du foie qui peuvent arrêter la thérapie immunosuppressive et que les cellules immunitaires innées sont susceptibles de jouer un rôle majeur dans le maintien d'une tolérance opérationnelle de la transplantation du foie.
EP09772548A 2008-07-03 2009-07-03 Procédé de diagnostic/pronostic in vitro et kit pour évaluer la tolérance de la transplantation du foie Withdrawn EP2313519A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP09772548A EP2313519A1 (fr) 2008-07-03 2009-07-03 Procédé de diagnostic/pronostic in vitro et kit pour évaluer la tolérance de la transplantation du foie

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
PCT/EP2008/058592 WO2010000320A1 (fr) 2008-07-03 2008-07-03 Procédé et kit de diagnostic/pronostic in vitro pour l'évaluation de la tolérance dans une transplantation hépatique
PCT/EP2009/058441 WO2010000848A1 (fr) 2008-07-03 2009-07-03 Procédé de diagnostic/pronostic in vitro et kit pour évaluer la tolérance de la transplantation du foie
EP09772548A EP2313519A1 (fr) 2008-07-03 2009-07-03 Procédé de diagnostic/pronostic in vitro et kit pour évaluer la tolérance de la transplantation du foie

Publications (1)

Publication Number Publication Date
EP2313519A1 true EP2313519A1 (fr) 2011-04-27

Family

ID=43760036

Family Applications (1)

Application Number Title Priority Date Filing Date
EP09772548A Withdrawn EP2313519A1 (fr) 2008-07-03 2009-07-03 Procédé de diagnostic/pronostic in vitro et kit pour évaluer la tolérance de la transplantation du foie

Country Status (1)

Country Link
EP (1) EP2313519A1 (fr)

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO2010000848A1 *

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Inventor name: LOZANO SALVATELLA, JUAN JOSE

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