EP2298449B1 - Dispositif pour analyse - Google Patents

Dispositif pour analyse Download PDF

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Publication number
EP2298449B1
EP2298449B1 EP10183084A EP10183084A EP2298449B1 EP 2298449 B1 EP2298449 B1 EP 2298449B1 EP 10183084 A EP10183084 A EP 10183084A EP 10183084 A EP10183084 A EP 10183084A EP 2298449 B1 EP2298449 B1 EP 2298449B1
Authority
EP
European Patent Office
Prior art keywords
chamber
fluid
channel
components
heat
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Not-in-force
Application number
EP10183084A
Other languages
German (de)
English (en)
Other versions
EP2298449A2 (fr
EP2298449A3 (fr
Inventor
Hans-Peter Wahl
Emad Sarofim
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Roche Diagnostics GmbH
Original Assignee
F Hoffmann La Roche AG
Roche Diagnostics GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG, Roche Diagnostics GmbH filed Critical F Hoffmann La Roche AG
Priority to EP10183084A priority Critical patent/EP2298449B1/fr
Publication of EP2298449A2 publication Critical patent/EP2298449A2/fr
Publication of EP2298449A3 publication Critical patent/EP2298449A3/fr
Application granted granted Critical
Publication of EP2298449B1 publication Critical patent/EP2298449B1/fr
Not-in-force legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50851—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates specially adapted for heating or cooling samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/04—Exchange or ejection of cartridges, containers or reservoirs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B01L2300/0858—Side walls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0677—Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples

Definitions

  • the present invention relates to a fluidic device for analysis of a fluid, said device having a plurality of analytical units, a method of use of said device, an instrument for analyzing fluids using said device and a system including said device and said instrument.
  • the field of application of the fluidic device according to the invention is mainly in analytics of fluid, for instance in health care, for the analysis of nucleic acids. Analyses performed using this device are considerably improved, as it is possible to increase the throughput of samples to be analyzed in automated manner.
  • EP 318 256 there is shown a device comprising a chamber through which the fluid is forced. This device cannot perform more than one analysis.
  • a fluidic unit is a part of the device that has a fluidic behavior which is independent from the individual fluidic behavior of other fluidic units in the device.
  • the function of fluidic units of the same device may be the same or different. However, preferably the function is generally the same, but the reagents used for the treatment may differ from one fluidic unit to another.
  • the geometry of the fluidic units on the same device is the same, e.g. each unit has the same number and arrangement of chambers and channels as any other fluidic unit on the same device.
  • the fluidic units allow the analysis of different fluids in parallel. For example, two or more samples from different sources, e.g. from different human beings, but of the same kind, e.g. serum, can be analyzed in parallel.
  • the body has an area that is generally flat over an area of between 100 and 10000 mm 2 , more preferred between 160 and 2250 mm 2 or more preferably between 4500 and 8500 mm 2 .
  • This area is in the following called the sealing area.
  • the term flat means that the body towards the outside of the device is as geometrically homogenous to allow a sealing unit to approach and thermally contact the body such that sufficient heat can be applied to the material of the body to melt a part of the body in contact with the sealing unit.
  • the body may contain areas that rise from the flat surface, e.g. in the vicinity of chambers formed in the body.
  • a channel according to the invention is a cavity in the device which has a longitudinal dimension that is larger than its width and height.
  • the channel is preferably confined by walls defining the width and height of the channel.
  • a wall of the channel is defined by the surface of a groove formed in the body of the device and a surface of a wall closely sealed to the edges of the grooves of the body.
  • Channels formed within the device preferably have a cross section of less than 10 mm 2 , preferably of between 0.01 and 2 mm 2 .
  • Channels for transporting fluids through the device will preferably have smaller dimensions than chambers for keeping the fluids or/and performing a process, preferably a chemical reaction.
  • a chamber is used to receive a sample having a large volume, e.g. for performing a lysis reaction in the original sample, adding a certain volume of reagent fluid.
  • the volume of a chamber may be less than 1 L, preferably between 50 ⁇ l and 10 ml.
  • the lysis chamber is a chamber for chemical sample preparation, such as the lysis of cellular components of a fluid containing cells to release constituents of said cells, e.g. nucleic acids or other substances to be analyzed.
  • a lysis chamber does not need to be a flat chamber, but preferably will have an at least partially tube like form, having an upper opening for introducing a sample and reagents for lysis, and a lower opening as an outlet to a channel. Conditions under which chemical sample preparation is performed are well known and can be applied to the present invention easily.
  • recognition is the recognition of the group of nucleic acids by glass surfaces under binding conditions.
  • a glass fleece is preferred.
  • a chamber for separating nucleic acids from a fluid will preferably have a volume of between 5 ⁇ l and 100 ⁇ l.
  • Conditions for binding nucleic acids are well known to those skilled in the art and may comprise the use of chaotropic salts, such as guanidinium isocyanate.
  • the fluidic units are preferably arranged in a parallel mode. This means that the positions of the chambers and channels of different fluidic units geometrically parallel each other. Any inlet and outlet ports will then be located at the same side of the device, preferably each kind of port, e.g. the inlet ports, along an edge of the device, the other kind, e.g. the outlet ports, being located along another edge. If there are two different kinds of inlet ports, they may be arranged at the same side or edge of the device.
  • the two parts - body and sealing wall- can be joined by known methods.
  • the sealing wall is a thin wall comprising a thermoplastic polymer and the rigid body is made of polymer, e.g. polystyrene or polypropylene
  • the two parts can be combined and then sealed by welding, for example Laser welding, ultrasound welding, thermo sealing or gluing.
  • the two parts can also just be clamped or stuck together.
  • the fluids preferably samples to be analyzed or/and reagents
  • they are introduced into the fluidic units by a head as outlined above for the instrument, e.g. such as a head carrying pipette tips, through said inlet ports into the first chambers.
  • the samples are treated to set the components of the samples to be analyzed free from any cellular compartments the components may be associated with in the samples.
  • this may include disrupting cells by a combination of chemical treatment with chaotropic salts and a protease to digest cell walls with a physical treatment with heat, e.g.
  • the method preferably comprises first washing the components immobilized on the porous material and then eluting them from the material.
  • the liquids needed for that are conveniently introduced into said second chambers through said inlet channels of the preferred embodiment (see also FIG 2a ).

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Claims (7)

  1. Système d'analyse d'un fluide dans un dispositif, comprenant
    a) un dispositif comprenant un corps rigide comprenant deux unités fluidiques ou plus étant fermées de façon étanche par une paroi d'étanchéité, chaque unité fluidique comprenant
    - une chambre de lyse pour la lyse de cellules dans un fluide pour libérer des composants contenus dans celui-ci,
    - une chambre de liaison comprenant une phase solide pour immobilisation de composants destinés à être analysés, ladite chambre de liaison étant connectée à ladite chambre de lyse par l'intermédiaire d'un premier canal,
    - une chambre de détection ayant une paroi au moins partiellement transparente pour analyser des composants de fluide, ladite chambre de détection étant connectée à ladite chambre de liaison par l'intermédiaire d'un deuxième canal,
    dans lequel la paroi d'étanchéité est une paroi de transfert de chaleur pour transférer une chaleur vers le et/ou du corps de dispositif et/ou fluide contenu dans celui-ci,
    b) un instrument analytique comprenant
    - un accessoire pour maintenir le dispositif,
    - une tête comprenant deux unités ou plus de manipulation de liquide pour distribuer des fluides dans le et/ou aspirer des fluides du dispositif,
    - un élément de chauffage et/ou de refroidissement pour chacune des unités fluidiques du dispositif et en contact avec la paroi de transfert de chaleur,
    - une unité de surveillance de propriétés optiquement connectée à la partie transparente de la paroi de ladite chambre de détection.
  2. Système selon la revendication 1 dans lequel une paroi de transfert de chaleur a un débit de transfert de chaleur supérieur à 200 W/m2/K pour transférer une chaleur vers le et/ou du corps de dispositif et/ou fluide contenu dans celui-ci.
  3. Système selon l'une quelconque des revendications précédentes, dans lequel la paroi de transfert de chaleur est thermiquement discontinue entre les unités fluidiques.
  4. Système selon l'une quelconque des revendications précédentes dans lequel chacun des éléments de chauffage et/ou de refroidissement est régulé indépendamment.
  5. Procédé d'analyse de composants de plus d'un fluide comprenant les étapes de
    - prévision d'un système selon l'une quelconque des revendications 1 à 4, et
    - dans les unités fluidiques
    - introduction d'un fluide dans ladite chambre de lyse,
    - libération dudit composant dudit fluide à partir d'autres composants dudit fluide avec lesquels ce composant est associé dans ladite chambre de lyse,
    - transfert du fluide résultant à travers ledit premier canal jusque dans la chambre de liaison pour liaison des composants destinés à être analysés à la phase solide,
    - introduction d'un tampon de lavage à l'intérieur de ladite chambre de liaison à travers ledit premier canal ou canal latéral pour éliminer des composants libres des fluides des composants immobilisés,
    - introduction d'un tampon d'élution dans ladite chambre de liaison à travers ledit premier canal ou canal latéral pour éluer les composants liés et transférer lesdits composants dans la chambre de détection à travers ledit deuxième canal,
    - traitement thermique du fluide contenant les composants élués dans ladite chambre de détection à travers ladite paroi de transfert de chaleur et analyse desdits composants dans la chambre de détection par l'intermédiaire de la chambre de détection transparente.
  6. Procédé selon la revendication 5 comprenant l'étape de fermeture de vannes par traitement thermique.
  7. Procédé selon la revendication 5 ou 6 comprenant l'étape d'utilisation de différents profils thermiques au niveau d'unités fluidiques adjacentes.
EP10183084A 2006-07-14 2007-07-13 Dispositif pour analyse Not-in-force EP2298449B1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP10183084A EP2298449B1 (fr) 2006-07-14 2007-07-13 Dispositif pour analyse

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP06014678A EP1886727A1 (fr) 2006-07-14 2006-07-14 Dispositif pour analyse
EP10183084A EP2298449B1 (fr) 2006-07-14 2007-07-13 Dispositif pour analyse
EP07765203A EP2054155B1 (fr) 2006-07-14 2007-07-13 Dispositif pour analyse

Related Parent Applications (2)

Application Number Title Priority Date Filing Date
EP07765203.0 Division 2007-07-13
EP07765203A Division-Into EP2054155B1 (fr) 2006-07-14 2007-07-13 Dispositif pour analyse

Publications (3)

Publication Number Publication Date
EP2298449A2 EP2298449A2 (fr) 2011-03-23
EP2298449A3 EP2298449A3 (fr) 2011-05-11
EP2298449B1 true EP2298449B1 (fr) 2013-01-02

Family

ID=37622007

Family Applications (3)

Application Number Title Priority Date Filing Date
EP06014678A Withdrawn EP1886727A1 (fr) 2006-07-14 2006-07-14 Dispositif pour analyse
EP07765203A Not-in-force EP2054155B1 (fr) 2006-07-14 2007-07-13 Dispositif pour analyse
EP10183084A Not-in-force EP2298449B1 (fr) 2006-07-14 2007-07-13 Dispositif pour analyse

Family Applications Before (2)

Application Number Title Priority Date Filing Date
EP06014678A Withdrawn EP1886727A1 (fr) 2006-07-14 2006-07-14 Dispositif pour analyse
EP07765203A Not-in-force EP2054155B1 (fr) 2006-07-14 2007-07-13 Dispositif pour analyse

Country Status (3)

Country Link
EP (3) EP1886727A1 (fr)
JP (1) JP2009543548A (fr)
WO (1) WO2008006617A2 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0715170D0 (en) 2007-08-03 2007-09-12 Enigma Diagnostics Ltd Reaction vessel
WO2012019119A1 (fr) * 2010-08-06 2012-02-09 Instantlabs Medical Diagnostics Corporation Systèmes, dispositifs et procédés de surveillance et de détection de réactions chimiques
EP2647435B1 (fr) 2012-04-05 2020-08-05 ThinXXS Microtechnology AG Système avec une cellule fluidique et un élément de températion
US9428787B2 (en) 2012-06-05 2016-08-30 3M Innovative Properties Company Apparatus and method for processing a sample
US9700887B2 (en) 2012-06-05 2017-07-11 3M Innovative Properties Company Multiwell plate

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
CA1339653C (fr) 1986-02-25 1998-02-03 Larry J. Johnson Appareil servant a l'amplification automatisee de sequences nucleotidiques et d'essais au moyen de cycle de chauffage et de refroidissement
EP0318256B1 (fr) 1987-11-23 1992-12-23 EASTMAN KODAK COMPANY (a New Jersey corporation) Cuvette munie d'une paroi rigide conductrice de chaleur
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
EP0637997B1 (fr) 1992-05-01 1998-07-01 Trustees Of The University Of Pennsylvania Analyse fondee sur un resserrement du systeme d'ecoulement
US5863502A (en) * 1996-01-24 1999-01-26 Sarnoff Corporation Parallel reaction cassette and associated devices
WO2002043864A2 (fr) * 2000-11-03 2002-06-06 Clinical Micro Sensors, Inc. Dispositifs et procedes de multiplexage de puces a adn
US7217542B2 (en) * 2002-10-31 2007-05-15 Hewlett-Packard Development Company, L.P. Microfluidic system for analyzing nucleic acids
WO2006044368A1 (fr) * 2004-10-12 2006-04-27 Bioprocessors Corp. Microréacteurs incorporant des polymères interpénétrants et/ou semi-interpénétrants

Also Published As

Publication number Publication date
EP2054155A2 (fr) 2009-05-06
EP2298449A2 (fr) 2011-03-23
JP2009543548A (ja) 2009-12-10
EP1886727A1 (fr) 2008-02-13
EP2054155B1 (fr) 2012-10-17
EP2298449A3 (fr) 2011-05-11
WO2008006617A2 (fr) 2008-01-17
WO2008006617A3 (fr) 2008-06-12

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