EP2291541A4 - Multiplexed pcr-coupled pna labeled beads flow cytometric assay for simultaneous detection of multiple biological agents - Google Patents

Multiplexed pcr-coupled pna labeled beads flow cytometric assay for simultaneous detection of multiple biological agents

Info

Publication number
EP2291541A4
EP2291541A4 EP09716939A EP09716939A EP2291541A4 EP 2291541 A4 EP2291541 A4 EP 2291541A4 EP 09716939 A EP09716939 A EP 09716939A EP 09716939 A EP09716939 A EP 09716939A EP 2291541 A4 EP2291541 A4 EP 2291541A4
Authority
EP
European Patent Office
Prior art keywords
flow cytometric
biological agents
simultaneous detection
multiple biological
multiplexed pcr
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP09716939A
Other languages
German (de)
French (fr)
Other versions
EP2291541A1 (en
Inventor
Wan Wen Su
Jeffry Golden
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Clean Earth Technologies LLC
Original Assignee
Clean Earth Technologies LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Clean Earth Technologies LLC filed Critical Clean Earth Technologies LLC
Publication of EP2291541A1 publication Critical patent/EP2291541A1/en
Publication of EP2291541A4 publication Critical patent/EP2291541A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP09716939A 2008-03-05 2009-03-05 Multiplexed pcr-coupled pna labeled beads flow cytometric assay for simultaneous detection of multiple biological agents Withdrawn EP2291541A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US3395208P 2008-03-05 2008-03-05
PCT/US2009/001466 WO2009111072A1 (en) 2008-03-05 2009-03-05 Multiplexed pcr-coupled pna labeled beads flow cytometric assay for simultaneous detection of multiple biological agents

Publications (2)

Publication Number Publication Date
EP2291541A1 EP2291541A1 (en) 2011-03-09
EP2291541A4 true EP2291541A4 (en) 2011-06-22

Family

ID=41056320

Family Applications (1)

Application Number Title Priority Date Filing Date
EP09716939A Withdrawn EP2291541A4 (en) 2008-03-05 2009-03-05 Multiplexed pcr-coupled pna labeled beads flow cytometric assay for simultaneous detection of multiple biological agents

Country Status (2)

Country Link
EP (1) EP2291541A4 (en)
WO (1) WO2009111072A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102719564B (en) * 2012-06-25 2013-09-25 广西壮族自治区兽医研究所 Triple polymerase chain reaction (PCR) kit for duck hepatitis virus type I, duck circoviruses and Muscovy duckling parvovirosis and application of triple PCR kit
CN114341627A (en) * 2019-11-13 2022-04-12 李峰 Nucleic acid detection method and device

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6449562B1 (en) * 1996-10-10 2002-09-10 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and method
WO2007024840A2 (en) * 2005-08-22 2007-03-01 Critical Therapeutics, Inc. Method of quantitating nucleic acids by flow cytometry microparticle-based array
US20070231803A1 (en) * 2006-03-31 2007-10-04 Jensen Mark A Multiplex pcr mixtures and kits containing the same

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE295427T1 (en) * 1996-06-04 2005-05-15 Univ Utah Res Found MONITORING HYBRIDIZATION DURING PCR
US6280946B2 (en) * 1998-08-07 2001-08-28 Boston Probes, Inc. PNA probes, probe sets, methods and kits pertaining to the universal detection of bacteria and eucarya
US6994971B1 (en) * 1999-10-08 2006-02-07 University Of Utah Research Foundation Particle analysis assay for biomolecular quantification
US20050214825A1 (en) * 2000-02-07 2005-09-29 John Stuelpnagel Multiplex sample analysis on universal arrays
US20040161767A1 (en) * 2002-06-28 2004-08-19 Baldwin Brett R. Detection and quantification of aromatic oxygenase genes by real-time PCR
US20040038385A1 (en) * 2002-08-26 2004-02-26 Langlois Richard G. System for autonomous monitoring of bioagents
US7381547B2 (en) * 2004-04-28 2008-06-03 Tzam Diagnostics, Llc Methods and compositions to detect bacteria using multiplex PCR

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6449562B1 (en) * 1996-10-10 2002-09-10 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and method
WO2007024840A2 (en) * 2005-08-22 2007-03-01 Critical Therapeutics, Inc. Method of quantitating nucleic acids by flow cytometry microparticle-based array
US20070231803A1 (en) * 2006-03-31 2007-10-04 Jensen Mark A Multiplex pcr mixtures and kits containing the same

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO2009111072A1 *

Also Published As

Publication number Publication date
WO2009111072A1 (en) 2009-09-11
EP2291541A1 (en) 2011-03-09

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