Classifications

• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23K—FODDER
  • A23K20/00—Accessory food factors for animal feeding-stuffs
  • A23K20/10—Organic substances
  • A23K20/179—Colouring agents, e.g. pigmenting or dyeing agents
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23K—FODDER
  • A23K50/00—Feeding-stuffs specially adapted for particular animals
  • A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
  • A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry

Definitions

• the present invention relates to the use of canthaxanthin and canthaxanthin containing compounds for increasing the reddish colour of animal tissue.
• the invention relates to the use of canthaxanthin for colouring muscle tissue in birds, especially poultry.
• canthaxanthin may act as a potential vitamin A precursor and plays an important role as a free radical scavenger due to its antioxidant properties in poultry metabolism.
• canthaxanthin is used in regular poultry diets in amounts of 8 to 10 ppm.
• canthaxanthin in amounts of at least 10 ppm , preferably of 12 to 25 ppm, in a bird diet results in a significant colouration of muscle tissue.
• the invention relates to the use of canthaxanthin in amounts of at least 10 ppm in a diet for increasing the reddish color of animal tissue.
• the invention relates to the use of canthaxanthin in amounts of 10 to 25 ppm in a diet for colouration of muscle tissue in birds, especially poultry.
• the invention relates to an animal diet comprising at least 10 ppm canthaxanthin and to a method of colouring muscle tissue in animals, especially birds, which comprises administering to such animals an effective amount of at least 10 ppm canthaxanthin.
• birds as used herein comprises poultry, turkey and canard.
• canthaxanthin is suitably administered as supplement to the animal feed or diet.
• Feed or diet may be supplemented by admixing canthaxanthin, e.g., as a commercial formulation such as available under the Trademark CAROPHYLL®Red ® 10%, to regular feed or by first preparing a premix of a feed component and canthaxanthin and subsequent mixing the premix with other food components.
• the food can be any conventional bird feed.
• feed or diet as used herein comprises both solid and liquid food as well as drinking fluids such as drinking water.
• canthaxanthin can be added as a formulated powder to a premix containing other minerals, vitamins, amino acids and trace elements which premix is added to regular animal food and thorough mixing to achieve even distribution therein.
• Canthaxanthin was supplemented to a low carotenoid basal diet at increasing levels of 1.56, 3.13, 6.25, 12.5, 25.0 and 50 mg per kg feed. The supplementation was given over the whole test period from day 1 to day 36. Furthermore, birds of one treatment received 25 mg canthaxanthin per kg feed only in the last three weeks (day 15 - 36) of the experiment. A control diet was formulated without any supplementation of canthaxanthin.
• the room temperature was adapted to the age of the birds. In the first few days an additional infra-red light was placed in each pen. Feed and tap water were provided ad libitum. During the first week, the feed was given as crumbled afterwards as pelleted feed. The animals were weighed at the beginning of the test period (day 1), at day 15 and at the end of the trial (day 36). The feed consumption was determined per group. Body weight gain and feed conversion ratio were calculated.
• mice received a low carotenoid basal diet (Table 1).
• the diets of groups B - G were supplemented with canthaxanthin according the trial design during the five weeks of the trial.
• the animals of the treatment H received the canthaxanthin supplementation only from day 15 to day 36.
• the control treatment (groups A) did not contain any additional canthaxanthin.
• canthaxanthin (CAROPHYLL® Red 10%, (10.5% canthaxanthin)) was given at inclusion levels of 0, 1.56, 3.13, 6.25, 12.5, 25 and 50 mg per kg feed.
• the animals of the groups H received 25 mg canthaxanthin (CAROPHYLL® RED 10%, 10.4 % canthaxanthin) per kg feed.
• Appropriate amounts of the products were mixed with 1 kg of the basal feed as a premix which was then added to the final feed in order to achieve the targeted concentrations. After mixing, the feed was pelleted (3 x 25 mm) at a temperature of 70 0 C at the die.
• the concentration of carotenoids in the feed was determined by HPLC. Plasma, skin and abdominal fat were extracted and analysed for carotenoids by the HPLC method used for the in- feed assays. In the same way, the concentration of carotenoids in the breast muscle, liver and kidney samples were determined. The samples of the thigh muscles were stored at -20 0 C for an eventual canthaxanthin determination at a later stage.
• the concentration of canthaxanthin in the skin, in the abdominal fat and in the plasma samples are presented in Table 3, 4 and 5.
• canthaxanthin was included at 12.5, 25 or 50 mg per kg feed, the differences between the treatment groups were significant in the skin and in the abdominal fat compared to the lower dosages. In the plasma the effect was already significant at an inclusion level of 6.25 mg/kg compared to the lower dosage. Besides these significant effects of the canthaxanthin supplementation there was also a linear dose- dependent increase of the canthaxanthin concentration in the tissues ( Figures 1 to 3).
• Table 6 shows a comparison of the via HPLC assay determined contents in the skin, the abdominal fat and the blood plasma and the values calculated with the linear regression equations on the basis of three earlier conducted pigmentation studies in which canthaxanthin was added at levels of 0 - 20 mg/kg feed.
• the results of the analytical determination of the canthaxanthin content in skin, abdominal fat and plasma samples fully correspond to the data calculated with the above mentioned linear regression. Even at the highest level of canthaxanthin supplementation (50 mg/kg feed) the calculated level extrapolated from the regression was in accordance with the analysed level.
• Table 8 Determined concentration of canthaxanthin in the kindney samples (mean ⁇ stdv)

Landscapes

• Life Sciences & Earth Sciences (AREA)
• Chemical & Material Sciences (AREA)
• Polymers & Plastics (AREA)
• Birds (AREA)
• Animal Husbandry (AREA)
• Zoology (AREA)
• Engineering & Computer Science (AREA)
• Food Science & Technology (AREA)
• Feed For Specific Animals (AREA)
• Fodder In General (AREA)
• Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)

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• 2009
  • 2009-05-20 WO PCT/EP2009/056141 patent/WO2009141380A1/en active Application Filing
  • 2009-05-20 KR KR1020107028764A patent/KR20110015016A/ko not_active Application Discontinuation
  • 2009-05-20 EP EP09749870A patent/EP2285235A1/de not_active Withdrawn
  • 2009-05-20 JP JP2011509978A patent/JP2011520459A/ja not_active Withdrawn

Non-Patent Citations (1)

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