EP2285235A1 - Novel use of canthaxanthin - Google Patents

Novel use of canthaxanthin

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Publication number
EP2285235A1
EP2285235A1 EP09749870A EP09749870A EP2285235A1 EP 2285235 A1 EP2285235 A1 EP 2285235A1 EP 09749870 A EP09749870 A EP 09749870A EP 09749870 A EP09749870 A EP 09749870A EP 2285235 A1 EP2285235 A1 EP 2285235A1
Authority
EP
European Patent Office
Prior art keywords
canthaxanthin
feed
ppm
groups
animal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP09749870A
Other languages
German (de)
French (fr)
Inventor
Raffaella Aureli
Fidelis Fru
Jose-Maria Hernandez
Petra Philipps
Joseph Schierle
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DSM IP Assets BV
Original Assignee
DSM IP Assets BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DSM IP Assets BV filed Critical DSM IP Assets BV
Priority to EP09749870A priority Critical patent/EP2285235A1/en
Publication of EP2285235A1 publication Critical patent/EP2285235A1/en
Withdrawn legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/179—Colouring agents, e.g. pigmenting or dyeing agents
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
    • A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry

Definitions

  • the present invention relates to the use of canthaxanthin and canthaxanthin containing compounds for increasing the reddish colour of animal tissue.
  • the invention relates to the use of canthaxanthin for colouring muscle tissue in birds, especially poultry.
  • canthaxanthin may act as a potential vitamin A precursor and plays an important role as a free radical scavenger due to its antioxidant properties in poultry metabolism.
  • canthaxanthin is used in regular poultry diets in amounts of 8 to 10 ppm.
  • canthaxanthin in amounts of at least 10 ppm , preferably of 12 to 25 ppm, in a bird diet results in a significant colouration of muscle tissue.
  • the invention relates to the use of canthaxanthin in amounts of at least 10 ppm in a diet for increasing the reddish color of animal tissue.
  • the invention relates to the use of canthaxanthin in amounts of 10 to 25 ppm in a diet for colouration of muscle tissue in birds, especially poultry.
  • the invention relates to an animal diet comprising at least 10 ppm canthaxanthin and to a method of colouring muscle tissue in animals, especially birds, which comprises administering to such animals an effective amount of at least 10 ppm canthaxanthin.
  • birds as used herein comprises poultry, turkey and canard.
  • canthaxanthin is suitably administered as supplement to the animal feed or diet.
  • Feed or diet may be supplemented by admixing canthaxanthin, e.g., as a commercial formulation such as available under the Trademark CAROPHYLL®Red ® 10%, to regular feed or by first preparing a premix of a feed component and canthaxanthin and subsequent mixing the premix with other food components.
  • the food can be any conventional bird feed.
  • feed or diet as used herein comprises both solid and liquid food as well as drinking fluids such as drinking water.
  • canthaxanthin can be added as a formulated powder to a premix containing other minerals, vitamins, amino acids and trace elements which premix is added to regular animal food and thorough mixing to achieve even distribution therein.
  • Canthaxanthin was supplemented to a low carotenoid basal diet at increasing levels of 1.56, 3.13, 6.25, 12.5, 25.0 and 50 mg per kg feed. The supplementation was given over the whole test period from day 1 to day 36. Furthermore, birds of one treatment received 25 mg canthaxanthin per kg feed only in the last three weeks (day 15 - 36) of the experiment. A control diet was formulated without any supplementation of canthaxanthin.
  • the room temperature was adapted to the age of the birds. In the first few days an additional infra-red light was placed in each pen. Feed and tap water were provided ad libitum. During the first week, the feed was given as crumbled afterwards as pelleted feed. The animals were weighed at the beginning of the test period (day 1), at day 15 and at the end of the trial (day 36). The feed consumption was determined per group. Body weight gain and feed conversion ratio were calculated.
  • mice received a low carotenoid basal diet (Table 1).
  • the diets of groups B - G were supplemented with canthaxanthin according the trial design during the five weeks of the trial.
  • the animals of the treatment H received the canthaxanthin supplementation only from day 15 to day 36.
  • the control treatment (groups A) did not contain any additional canthaxanthin.
  • canthaxanthin (CAROPHYLL® Red 10%, (10.5% canthaxanthin)) was given at inclusion levels of 0, 1.56, 3.13, 6.25, 12.5, 25 and 50 mg per kg feed.
  • the animals of the groups H received 25 mg canthaxanthin (CAROPHYLL® RED 10%, 10.4 % canthaxanthin) per kg feed.
  • Appropriate amounts of the products were mixed with 1 kg of the basal feed as a premix which was then added to the final feed in order to achieve the targeted concentrations. After mixing, the feed was pelleted (3 x 25 mm) at a temperature of 70 0 C at the die.
  • the concentration of carotenoids in the feed was determined by HPLC. Plasma, skin and abdominal fat were extracted and analysed for carotenoids by the HPLC method used for the in- feed assays. In the same way, the concentration of carotenoids in the breast muscle, liver and kidney samples were determined. The samples of the thigh muscles were stored at -20 0 C for an eventual canthaxanthin determination at a later stage.
  • the concentration of canthaxanthin in the skin, in the abdominal fat and in the plasma samples are presented in Table 3, 4 and 5.
  • canthaxanthin was included at 12.5, 25 or 50 mg per kg feed, the differences between the treatment groups were significant in the skin and in the abdominal fat compared to the lower dosages. In the plasma the effect was already significant at an inclusion level of 6.25 mg/kg compared to the lower dosage. Besides these significant effects of the canthaxanthin supplementation there was also a linear dose- dependent increase of the canthaxanthin concentration in the tissues ( Figures 1 to 3).
  • Table 6 shows a comparison of the via HPLC assay determined contents in the skin, the abdominal fat and the blood plasma and the values calculated with the linear regression equations on the basis of three earlier conducted pigmentation studies in which canthaxanthin was added at levels of 0 - 20 mg/kg feed.
  • the results of the analytical determination of the canthaxanthin content in skin, abdominal fat and plasma samples fully correspond to the data calculated with the above mentioned linear regression. Even at the highest level of canthaxanthin supplementation (50 mg/kg feed) the calculated level extrapolated from the regression was in accordance with the analysed level.
  • Table 8 Determined concentration of canthaxanthin in the kindney samples (mean ⁇ stdv)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Animal Husbandry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Birds (AREA)
  • Fodder In General (AREA)
  • Feed For Specific Animals (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)

Abstract

The present invention relates to the use of canthaxanthin and canthaxanthin containing compounds for increasing the reddish colour of animal tissue. More particularly, the invention relates to the use of canthaxanthin for colouring muscle tissue in birds, especially poultry. It is well accepted that canthaxanthin may act as a potential vitamin A precursor and plays an important role as a free radical scavenger due to its antioxidant properties in poultry metabolism. For these purposes canthaxanthin is used in regular poultry diets in amounts of 8 to 10 ppm. Now, it has surprisingly been found that the use of canthaxanthin in amounts of at least 10 ppm, preferably of 12 to 25 ppm, in a bird diet results in a significant colouration of muscle tissue.

Description

Novel use of Canthaxanthin
The present invention relates to the use of canthaxanthin and canthaxanthin containing compounds for increasing the reddish colour of animal tissue.
More particularly, the invention relates to the use of canthaxanthin for colouring muscle tissue in birds, especially poultry.
It is well accepted that canthaxanthin may act as a potential vitamin A precursor and plays an important role as a free radical scavenger due to its antioxidant properties in poultry metabolism. For these purposes canthaxanthin is used in regular poultry diets in amounts of 8 to 10 ppm.
Now, it has surprisingly been found that the use of canthaxanthin in amounts of at least 10 ppm , preferably of 12 to 25 ppm, in a bird diet results in a significant colouration of muscle tissue.
Thus, in one aspect, the invention relates to the use of canthaxanthin in amounts of at least 10 ppm in a diet for increasing the reddish color of animal tissue.
In still another aspect, the invention relates to the use of canthaxanthin in amounts of 10 to 25 ppm in a diet for colouration of muscle tissue in birds, especially poultry.
In yet another aspect, the invention relates to an animal diet comprising at least 10 ppm canthaxanthin and to a method of colouring muscle tissue in animals, especially birds, which comprises administering to such animals an effective amount of at least 10 ppm canthaxanthin. The term "bird" as used herein comprises poultry, turkey and canard.
For the purposes of the invention, canthaxanthin is suitably administered as supplement to the animal feed or diet. Feed or diet may be supplemented by admixing canthaxanthin, e.g., as a commercial formulation such as available under the Trademark CAROPHYLL®Red ® 10%, to regular feed or by first preparing a premix of a feed component and canthaxanthin and subsequent mixing the premix with other food components. The food can be any conventional bird feed.
The term feed or diet as used herein comprises both solid and liquid food as well as drinking fluids such as drinking water. Particularly, canthaxanthin can be added as a formulated powder to a premix containing other minerals, vitamins, amino acids and trace elements which premix is added to regular animal food and thorough mixing to achieve even distribution therein.
The efficiency of the treatment of birds in accordance with the invention can be seen from the experiments described below.
Example: The effects of increasing amounts up to a level of 50 mg canthaxanthin per kg feed on the concentration of canthaxanthin in various tissues of broiler chickens
In a broiler pigmentation study deposition of canthaxanthin in various tissues was evaluated. Canthaxanthin was supplemented to a low carotenoid basal diet at increasing levels of 1.56, 3.13, 6.25, 12.5, 25.0 and 50 mg per kg feed. The supplementation was given over the whole test period from day 1 to day 36. Furthermore, birds of one treatment received 25 mg canthaxanthin per kg feed only in the last three weeks (day 15 - 36) of the experiment. A control diet was formulated without any supplementation of canthaxanthin.
The supplementation of the feed with increasing amounts of canthaxanthin up to levels of 50 mg per kg feed resulted in linearly increased concentrations of canthaxanthin in the broiler skin, abdominal fat, blood plasma, livers, kidneys and breast muscles. The canthaxanthin concentrations in the skin, the abdominal fat and the plasma fully confirm results of earlier studies. The supplementation of canthaxanthin over the whole trial period resulted in a significantly higher concentration of canthaxanthin in the skin and the breast muscles compared to the birds which received the same level of canthaxanthin only from day 15 to day 36.
Nevertheless, there were no differences found between these groups in tissue samples as abdominal fat, blood plasma, liver and kidney. In addition, a more intensive colouration of shanks and breast muscles was observed at increasing levels of 12.5, 25 and 50 mg canthaxanthin per kg feed.
Materials and Methods
Day-old broiler chickens were supplied by a commercial hatchery (Joseph Grelier S. A., Elevage avicole de Ia Bohadiere, F-49290 Saint-Laurent de Ia Plaine, France). The chickens were separated by sex. On the day of arrival (day 1), the chickens were divided by weight into groups of 20 birds, ten males and ten females. The females were identified with a red colour on the feathers. Each group was placed in one floor pen littered with wood shavings and allocated to one of the eight different dietary treatments. Each treatment was replicated with two groups (A = Al + A2, B = Bl + B2, ...). The chickens were housed in an environmentally controlled room. The room temperature was adapted to the age of the birds. In the first few days an additional infra-red light was placed in each pen. Feed and tap water were provided ad libitum. During the first week, the feed was given as crumbled afterwards as pelleted feed. The animals were weighed at the beginning of the test period (day 1), at day 15 and at the end of the trial (day 36). The feed consumption was determined per group. Body weight gain and feed conversion ratio were calculated.
All the animals received a low carotenoid basal diet (Table 1). The diets of groups B - G were supplemented with canthaxanthin according the trial design during the five weeks of the trial. The animals of the treatment H received the canthaxanthin supplementation only from day 15 to day 36. The control treatment (groups A) did not contain any additional canthaxanthin.
To groups B - G canthaxanthin (CAROPHYLL® Red 10%, (10.5% canthaxanthin)) was given at inclusion levels of 0, 1.56, 3.13, 6.25, 12.5, 25 and 50 mg per kg feed. The animals of the groups H received 25 mg canthaxanthin (CAROPHYLL® RED 10%, 10.4 % canthaxanthin) per kg feed. Appropriate amounts of the products were mixed with 1 kg of the basal feed as a premix which was then added to the final feed in order to achieve the targeted concentrations. After mixing, the feed was pelleted (3 x 25 mm) at a temperature of 700C at the die.
At the end of the trial (day 36), ten chickens per group (five of each sex) were selected at random, labelled and blood samples were collected from the jugular vein. The following day, the animals were sacrificed and the carcasses partially defeathered. Samples of the dorsal skin, of the breast muscles, the thigh muscles and the abdominal fat were collected. The samples were pooled for each group. In addition, the livers and kidneys were also collected and pooled.
The concentration of carotenoids in the feed was determined by HPLC. Plasma, skin and abdominal fat were extracted and analysed for carotenoids by the HPLC method used for the in- feed assays. In the same way, the concentration of carotenoids in the breast muscle, liver and kidney samples were determined. The samples of the thigh muscles were stored at -200C for an eventual canthaxanthin determination at a later stage.
For the statistical evaluation of the data, a one-factorial (inclusion level) analysis of variance was carried out using the software 'Stat Box Pro', version 5.0 (Grimmersoft, 1995). Where significant treatment effects were indicated at probability levels of p < 0.05 the differences among treatment means were compared using the Newman Keuls test.
Results and Discussion
The analysed concentrations of carotenoids in the feed samples are shown in Table 2. The results were in accordance with the target levels. An average recovery of 95% in the final feed was noted for the canthaxanthin products.
The concentration of canthaxanthin in the skin, in the abdominal fat and in the plasma samples are presented in Table 3, 4 and 5. When canthaxanthin was included at 12.5, 25 or 50 mg per kg feed, the differences between the treatment groups were significant in the skin and in the abdominal fat compared to the lower dosages. In the plasma the effect was already significant at an inclusion level of 6.25 mg/kg compared to the lower dosage. Besides these significant effects of the canthaxanthin supplementation there was also a linear dose- dependent increase of the canthaxanthin concentration in the tissues (Figures 1 to 3). For groups F, where 25 mg canthaxanthin per kg of feed was added through the whole trial period a significant higher concentration of canthaxanthin in the skin was found compared to groups H where the same amount of canthaxanthin was given only from day 15 to day 36. In the abdominal fat and the blood plasma samples no difference was found between these two particular groups.
Table 6 shows a comparison of the via HPLC assay determined contents in the skin, the abdominal fat and the blood plasma and the values calculated with the linear regression equations on the basis of three earlier conducted pigmentation studies in which canthaxanthin was added at levels of 0 - 20 mg/kg feed. The results of the analytical determination of the canthaxanthin content in skin, abdominal fat and plasma samples fully correspond to the data calculated with the above mentioned linear regression. Even at the highest level of canthaxanthin supplementation (50 mg/kg feed) the calculated level extrapolated from the regression was in accordance with the analysed level.
The concentrations of canthaxanthin in the liver, kidney and breast muscle are presented in Table 7, 8 and 9. When the canthaxanthin product was included with more than 6.25 mg per kg feed a significantly higher concentration of canthaxanthin in the kidneys and in the breast muscle was measured compared to the lower supplementation levels. In the liver, a significant difference of the canthaxanthin concentration was noted for more than 12.5 mg canthaxanthin per kg feed. Besides these significant effects of the canthaxanthin supplementation there was also a linear dose-dependent increase of the canthaxanthin concentration in the above mentioned tissues (Figures 4 to 6).
For groups F, where 25 mg canthaxanthin per kg of feed was added through the whole trial period a significantly higher concentration of canthaxanthin in the breast muscle was found compared to groups H where the same amount of canthaxanthin was given only from day 15 to day 36. In the liver and kidney samples no difference was found between these two particular groups.
The colouration of the breast muscle samples and the shanks of the broilers from the groups A - G are shown in figure 7. Especially the high inclusion levels (12,5 - 50 mg/kg of feed) led to a more intensive reddish colour of the muscle and the shanks. The supplementation of canthaxanthin to the diets was without any influence on the growth performance of the birds (Table 10).
It can be concluded from this trial that the supplementation of the feed with increasing levels of canthaxanthin up to levels of 50 mg per kg feed resulted in linearly increased concentrations of canthaxanthin in broiler tissues.
Increasing canthaxanthin supplementation led to an increased concentration not only in skin, blood plasma and abdominal fat, but also in the liver, kidney and breast muscle.
Similar to the increased concentration of canthaxanthin in the tissues, a more intensive colouration of the shanks and of the breast muscles was observed with increasing supplementation levels from 12.5 to 25 mg canthaxanthin per kg feed.
The supplementation of canthaxanthin over the whole trial period (groups F; 25 mg canthaxanthin/kg feed) resulted in a significantly higher concentration of canthaxanthin in the skin and the breast muscles compared to the birds of groups H which received the same level of canthaxanthin only from day 15 to day 36. Nevertheless, there were no differences found between these groups in the tissue samples fat, blood plasma, liver and kidney.
The supplementation of canthaxanthin to the diets was without any effect on the animals' performance.
Table 1 : Feed composition of the basal diet
1 Metabolisable energy N-∞IT-, calculated with EC-equation on the base of the calculated crude nutrients
Table 2: Determined concentration of carotenoids in the feed
.-, .., /-, D Jose of ^i • C „ant .,haxant .,hm .
Crroups Treatment Cantnaxantnm , ,, ,
( /■mg/ ;ki g \) ( vmg 5/kg 5;)
A Control 0 < 0.01
B Canthaxanthin 1.56 1.45
C Canthaxanthin 3.13 3.03
D Canthaxanthin 6.25 6.68
E Canthaxanthin 12.5 12.9
F Canthaxanthin 25.0 24.0
G Canthaxanthin 50.0 46.6
H Canthaxanthin 25.0 25.5
Table 3 : Determined concentration of canthaxanthin in the skin (mean ± stdv)
Doses of
Canthaxanthin
Groups Treatment Canthaxanthin (mg/kg) (mg/kg)
A Control 0 < 0.01 F -
B Canthaxanthin 1.56 0.11 EF ± 0.01
C Canthaxanthin 3.13 0.21 EF ± 0.034
D Canthaxanthin 6.25 0.53 E ± 0.022
E Canthaxanthin 12.5 1.01 D ± 0.117
F Canthaxanthin 25.0 2.59 B ± 0.165
G Canthaxanthin 50.0 4.54 A ± 0.728
H Canthaxanthin 25.0 2.04 C ± 0.148
Newman Keuls test: Means within a column, not sharing a common superscript, are significantly different (p < 0.05). Table 4: Determined concentration of canthaxanthin in the abdominal fat (mean ± stdv)
Doses of
Canthaxanthin
Groups Treatment Canthaxanthin (mg/kg) (mg/kg)
A Control 0 < 0.01 D -
B Canthaxanthin 1.56 0.15 D ± 0.034
C Canthaxanthin 3.13 0.29 D ± 0.034
D Canthaxanthin 6.25 0.59 D ± 0.043
E Canthaxanthin 12.5 1.14 C ± 0.146
F Canthaxanthin 25.0 2.46 B ± 0.125
G Canthaxanthin 50.0 4.58 A ± 0.832
H Canthaxanthin 25.0 2.16 B ± 0.178
Newman Keuls test: Means within a column, not sharing a common superscnpt, are significantly different (p < 0.05).
Table 5 : Determined concentration of canthaxanthin in the plasma samples (mean ± stdv)
Doses of
Canthaxanthin
Groups Treatment Canthaxanthin (mg/kg) (mg/kg)
A Control 0 < 0.01 F -
B Canthaxanthin 1.56 0.41 EF ± 0.048
C Canthaxanthin 3.13 0.77 E ± 0.058
D Canthaxanthin 6.25 1.64 D ± 0.159
E Canthaxanthin 12.5 3.66 C ± 0.204
F Canthaxanthin 25.0 6.94 B ± 0.401
G Canthaxanthin 50.0 13.23 A ± 0.854
H Canthaxanthin 25.0 7.31 B ± 0.361
Newman Keuls test: Means withm a column, not sharing a common superscript, are significantly different (p < 0.05). Table 6: Determined concentrations of canthaxanthin in the skin, the abdominal fat and the plasma samples compared to calculations based on linear regression equations (mean ± stdv)
Dose of
Skin Abdominal fat Plasma samples
Group Treatment Canthax.. (mg/kg) (mg/kg) (mg/kg) (mg/kg) analysed calculatedl analysed calculated2 analysed calculated3
B Canthaxanthin 1.56 0.11 0.16 0.15 0.19 0.41 0.50
C Canthaxanthin 3.13 0.21 0.30 0.29 0.35 0.77 0.92
D Canthaxanthin 6.25 0.53 0.59 0.59 0.65 1.64 1.75
Canthaxanthin 12.5 1.01 1.18 1.14 1.25 3.66 3.42
Canthaxanthin 25.0 2.59 2.35* 2.46 2.46* 6.94 6.75*
G Canthaxanthin 50.0 4.54 4.68* 4.58 4.88* 13.2 13.4*
1: y = 0.0934x + 0.0107, R2 = 0.79 2: Y = 0.0968x + 0.0427, R2 = 0.75 3: Y = 0.2665x + 0.086, R2 = 0.92 * Extrapolated from regression equations
Table 7: Determined concentration of canthaxanthin in the liver samples (mean ± stdv)
Doses of
Canthaxanthin
Groups Treatment Canthaxanthin (mg/kg) (mg/kg)
A Control 0 < 0.01 C -
B Canthaxanthin 1.56 0.67 C ± 0.15
C Canthaxanthin 3.13 1.17 C ± 0.13
D Canthaxanthin 6.25 2.96 C ± 0.75
E Canthaxanthin 12.5 4.89 C ± 1.20
F Canthaxanthin 25.0 12.57 B ± 1.99
G Canthaxanthin 50.0 28.71 A ± 10.42
H Canthaxanthin 25.0 13.29 B ± 0.637 Newman Keuls test: Means within a column, not sharing a common superscript, are significantly different (p < 0.05).
Table 8: Determined concentration of canthaxanthin in the kindney samples (mean ± stdv)
Doses of
Canthaxanthin
Groups Treatment Canthaxanthin (mg/kg) (mg/kg)
A Control 0 < 0.01 D -
B Canthaxanthin 1.56 0.17 D ± 0.03
C Canthaxanthin 3.13 0.33 D ± 0.06
D Canthaxanthin 6.25 0.85 D ± 0.02
E Canthaxanthin 12.5 2.09 C ± 0.26
F Canthaxanthin 25.0 4.95 B ± 0.47
G Canthaxanthin 50.0 10.29 A ± 1.71
H Canthaxanthin 25.0 4.97 B ± 0.49
Newman Keuls test: Means within a column, not sharing a common superscript, are significantly different (p < 0.05).
Table 9: Determined concentration of canthaxanthin in the breast muscle samples (mean ± stdv)
Doses of
Canthaxanthin
Groups Treatment Canthaxanthin (mg/kg) (mg/kg)
A Control 0 < 0.01 E -
B Canthaxanthin 1.56 0.06 E ± 0.01
C Canthaxanthin 3.13 0.12 E ± 0.03
D Canthaxanthin 6.25 0.30 E ± 0.03
E Canthaxanthin 12.5 0.56 D ± 0.10
F Canthaxanthin 25.0 1.41 B ± 0.24
G Canthaxanthin 50.0 2.30 A ± 0.34
H Canthaxanthin 25.0 1.11 C ± 0.26
Newman Keuls test: Means within a column, not sharing a common superscript, are significantly different (p < 0.05). Table 10: Performance of broiler chickens (mean ± stdv) [MC-01/07]
A Control 0 2228 A ±24.6 1.624 A ±0.007
B Canthaxanthin 1.56 2325 A ± 16.7 1.577 A ±0.012
C Canthaxanthin 3.13 2236 A ±23.8 1.581 A ±0.014
D Canthaxanthin 6.25 2258 A ± 14.3 1.563 A ±0.029
E Canthaxanthin 12.5 2260 A ± 140.8 1.600 A ±0.089
F Canthaxanthin 25.0 2225 A ± 14.7 1.622 A ±0.007
G Canthaxanthin 50.0 2222 A ±33.6 1.609 A ±0.009
H Canthaxanthin 25.0 2189 A ±23.0 1.662 A ±0.006
Newman Keuls test: Means within a column, not sharing a common superscript, are significantly different (p < 0,05).

Claims

Claims
1. Use of canthaxanthin in amounts of at least 10 ppm in a bird diet for increasing the reddish colour of animal tissue.
2. Use according to claim 1 for colouring muscle tissue in birds
3. Use according to claim 1 or 2, wherein the animal is a bird, preferably poultry.
4. Use according to any of claims 1 to 3, wherein canthaxanthin is feed to the animal in amounts of at least 12 to 25 ppm.
5. Animal diet or feed comprising at least 10 ppm canthaxanthin for increasing the reddish colour of animal tissue.
6. Animal diet or feed according to claim 5, which is a poultry diet.
7. A method of colouring muscle tissue in animals, especially birds, which comprises administering to such animals an effective amount of at least 10 ppm canthaxanthin.
8. Method according to claim 7, which comprises administering to the animal an effective amount of 12 to 25 ppm of canthaxanthin.
EP09749870A 2008-05-22 2009-05-20 Novel use of canthaxanthin Withdrawn EP2285235A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP09749870A EP2285235A1 (en) 2008-05-22 2009-05-20 Novel use of canthaxanthin

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP08009430 2008-05-22
PCT/EP2009/056141 WO2009141380A1 (en) 2008-05-22 2009-05-20 Novel use of canthaxanthin
EP09749870A EP2285235A1 (en) 2008-05-22 2009-05-20 Novel use of canthaxanthin

Publications (1)

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Publication number Priority date Publication date Assignee Title
EP2938205A1 (en) * 2012-12-27 2015-11-04 DSM IP Assets B.V. Novel use of canthaxanthin and 25-hydroxy vitamin d3

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Publication number Priority date Publication date Assignee Title
US3539686A (en) * 1967-04-10 1970-11-10 Ralph Rosenberg Pigmentation in poultry husbandry
DE10104494A1 (en) * 2001-01-31 2002-08-01 Basf Ag Process for the preparation of dry powders of one or more carotenoids

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Title
See references of WO2009141380A1 *

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