EP2223086A4 - FLUORESCENT COMPOUNDS - Google Patents

FLUORESCENT COMPOUNDS

Info

Publication number
EP2223086A4
EP2223086A4 EP20080861025 EP08861025A EP2223086A4 EP 2223086 A4 EP2223086 A4 EP 2223086A4 EP 20080861025 EP20080861025 EP 20080861025 EP 08861025 A EP08861025 A EP 08861025A EP 2223086 A4 EP2223086 A4 EP 2223086A4
Authority
EP
European Patent Office
Prior art keywords
fluorescent compounds
fluorescent
compounds
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP20080861025
Other languages
German (de)
French (fr)
Other versions
EP2223086B2 (en
EP2223086A1 (en
EP2223086B1 (en
Inventor
Fei Mao
Wai-Yee Leung
Ching-Ying Cheung
Hye Eun Hoover
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biotium Inc
Original Assignee
Biotium Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=40795823&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=EP2223086(A4) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Biotium Inc filed Critical Biotium Inc
Publication of EP2223086A1 publication Critical patent/EP2223086A1/en
Publication of EP2223086A4 publication Critical patent/EP2223086A4/en
Application granted granted Critical
Publication of EP2223086B1 publication Critical patent/EP2223086B1/en
Publication of EP2223086B2 publication Critical patent/EP2223086B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B11/00—Diaryl- or thriarylmethane dyes
    • C09B11/04—Diaryl- or thriarylmethane dyes derived from triarylmethanes, i.e. central C-atom is substituted by amino, cyano, alkyl
    • C09B11/06—Hydroxy derivatives of triarylmethanes in which at least one OH group is bound to an aryl nucleus and their ethers or esters
    • C09B11/08—Phthaleins; Phenolphthaleins; Fluorescein
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B11/00—Diaryl- or thriarylmethane dyes
    • C09B11/04—Diaryl- or thriarylmethane dyes derived from triarylmethanes, i.e. central C-atom is substituted by amino, cyano, alkyl
    • C09B11/10—Amino derivatives of triarylmethanes
    • C09B11/24—Phthaleins containing amino groups ; Phthalanes; Fluoranes; Phthalides; Rhodamine dyes; Phthaleins having heterocyclic aryl rings; Lactone or lactame forms of triarylmethane dyes
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/0066—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain being part of a carbocyclic ring,(e.g. benzene, naphtalene, cyclohexene, cyclobutenene-quadratic acid)
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
    • C09B23/06—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups three >CH- groups, e.g. carbocyanines
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
    • C09B23/08—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups more than three >CH- groups, e.g. polycarbocyanines
    • C09B23/083—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups more than three >CH- groups, e.g. polycarbocyanines five >CH- groups
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
    • C09B23/08—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups more than three >CH- groups, e.g. polycarbocyanines
    • C09B23/086—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups more than three >CH- groups, e.g. polycarbocyanines more than five >CH- groups
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B57/00—Other synthetic dyes of known constitution
    • C09B57/001—Pyrene dyes
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B57/00—Other synthetic dyes of known constitution
    • C09B57/02—Coumarine dyes
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B69/00—Dyes not provided for by a single group of this subclass
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Cell Biology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Polyethers (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
EP08861025.8A 2007-12-14 2008-12-12 Fluorescent compounds - labeling dyes Active EP2223086B2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US1395607P 2007-12-14 2007-12-14
PCT/US2008/013698 WO2009078970A1 (en) 2007-12-14 2008-12-12 Fluorescent compounds

Publications (4)

Publication Number Publication Date
EP2223086A1 EP2223086A1 (en) 2010-09-01
EP2223086A4 true EP2223086A4 (en) 2011-05-04
EP2223086B1 EP2223086B1 (en) 2013-03-20
EP2223086B2 EP2223086B2 (en) 2016-06-01

Family

ID=40795823

Family Applications (1)

Application Number Title Priority Date Filing Date
EP08861025.8A Active EP2223086B2 (en) 2007-12-14 2008-12-12 Fluorescent compounds - labeling dyes

Country Status (5)

Country Link
US (3) US9097667B2 (en)
EP (1) EP2223086B2 (en)
JP (2) JP2011506673A (en)
CN (1) CN101946171B (en)
WO (1) WO2009078970A1 (en)

Families Citing this family (82)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001294859A1 (en) * 2000-09-29 2002-04-08 Molecular Probes, Inc. Modified carbocyanine dyes and their conjugates
CN101946171B (en) 2007-12-14 2014-03-12 拜奥蒂乌姆股份有限公司 Fluorescent compounds
US8436170B2 (en) * 2009-02-03 2013-05-07 Biotium, Inc. Xanthene dyes comprising a sulfonamide group
JP5515529B2 (en) * 2009-09-04 2014-06-11 国立大学法人三重大学 Near-infrared photoluminescent compound, synthesis method thereof and luminescence method thereof
US8658434B2 (en) * 2009-10-28 2014-02-25 Biotium, Inc. Fluorescent pyrene compounds
WO2011068569A1 (en) 2009-12-04 2011-06-09 Biotium, Inc. Heterocycle-substituted xanthene dyes
DE102010022110A1 (en) * 2010-05-31 2011-12-01 LMU Universität München Department Chemie Cyanine dyes as a contrast agent to aid eye surgery
EP2609426B1 (en) * 2010-08-27 2018-12-19 The Texas A&M University System Fluorescence labeling reagents and uses thereof
WO2012054749A1 (en) 2010-10-20 2012-04-26 Li-Cor, Inc. Cyanine dyes and their conjugates
FR2967675B1 (en) * 2010-11-24 2015-02-27 Pf Medicament FLUORESCENT DERIVATIVES OF POLYAMINATED CYANINES AS A DIAGNOSTIC PROBE
EP3029027B1 (en) 2010-12-21 2018-06-27 Pierce Biotechnology, Inc. Fluorescent compounds
FR2973030B1 (en) 2011-03-25 2013-10-18 Centre Nat Rech Scient COMPOUNDS WITH CHROMOPHORE AND PHOTOREACTIVE GROUPS
DE102011101207B3 (en) * 2011-05-11 2012-05-10 Sartorius Stedim Biotech Gmbh Fluorescent dye for pH sensor
JP5742494B2 (en) * 2011-06-10 2015-07-01 住友化学株式会社 Polymer compound and electronic device using the same
US8889884B1 (en) 2011-07-14 2014-11-18 Pierce Biotechnology, Inc. Phosphine derivatives of fluorescent compounds
US9249307B2 (en) 2011-08-16 2016-02-02 Pierce Biotechnology, Inc. Benzocyanine compounds
WO2013056720A1 (en) * 2011-10-18 2013-04-25 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. Fluorescent dyes with phosphorylated hydroxymethyl groups and their use in light microscopy and imaging techniques
CN102558909B (en) * 2011-12-15 2013-07-31 合肥工业大学 Hydroxy-terminated polyether type macromolecular fluorescent dye and synthesis method thereof
US8835000B2 (en) 2011-12-23 2014-09-16 General Electric Company High-density fluorescent dye clusters
CN102608052B (en) * 2012-02-21 2014-11-05 中国科学院化学研究所 System for diagnosis of leukemia
CN102608051B (en) * 2012-02-21 2015-04-08 中国科学院化学研究所 Reagent kit for diagnosis of leukemia
CN102706842B (en) * 2012-05-24 2015-04-08 中国科学院化学研究所 Kit and system for diagnosing leukemia
US9751868B2 (en) * 2012-02-28 2017-09-05 Pierce Biotechnology, Inc. Benzocyanine compounds
US20140072515A9 (en) 2012-03-02 2014-03-13 Greg Hermanson Cyanine compounds
CN103374234B (en) * 2012-04-11 2016-03-30 中国科学院理化技术研究所 Coumarin-based near-infrared fluorescent dye and preparation method and application thereof
CN104470546B (en) * 2012-07-20 2018-02-27 佳能株式会社 Compound and the photoacoustic imaging contrast agent containing the compound
US9676787B2 (en) 2012-08-28 2017-06-13 Pierce Biotechnology, Inc. Benzopyrylium compounds
US10107799B2 (en) 2012-09-18 2018-10-23 Biotium, Inc. Methods, compositions and kits for labeling of proteins
ES2628529T3 (en) 2012-10-24 2017-08-03 Cyanine-azaindoline dyes substituted with hydroxamate, and bioconjugates thereof
US20150182518A1 (en) * 2012-10-26 2015-07-02 Canon Kabushiki Kaisha Cancer cell inhibitory drug and cancer stem-cell detection probe
US20140255312A1 (en) * 2013-03-07 2014-09-11 Greg Hermanson Cyanine compounds
AU2013380645B2 (en) * 2013-03-08 2018-03-15 Illumina Cambridge Ltd Polymethine compounds and their use as fluorescent labels
EP2970674B1 (en) 2013-03-15 2018-12-12 VisEn Medical, Inc. 4,4-disubstituted cyclohexyl bridged heptamethine cyanine dyes and uses thereof
JP6456592B2 (en) * 2013-07-10 2019-01-23 国立大学法人 東京大学 Fluorescence lifetime imaging probe
EP3074482B1 (en) * 2013-11-27 2019-09-18 University of Washington through its Center for Commercialization Encoded chromophoric polymer particles
CN103642261A (en) * 2013-12-11 2014-03-19 南京晓庄学院 Novel Alexa Fluor fluorescent cyanine dye as well as preparation method and application thereof
US10487089B2 (en) 2014-03-21 2019-11-26 The Trustees Of The University Of Pennsylvania Imaging agents and methods of identifying same
US10245329B2 (en) * 2014-09-08 2019-04-02 Canon Kabushiki Kaisha Composition having dye and conjugate of polyethyleneglycol and additive and contrast agent for photoacoustic imaging having the same
JP6700750B2 (en) 2014-12-09 2020-05-27 キヤノン株式会社 Compound and contrast agent for optical imaging containing the compound
CN104710978A (en) * 2015-02-11 2015-06-17 深圳市前海安测信息技术有限公司 Organic electroluminescent material and preparation method thereof
US9670318B2 (en) 2015-05-28 2017-06-06 Miltenyi Biotec Gmbh Bright fluorochromes based on multimerization of fluorescent dyes
EP3098269B1 (en) 2015-05-28 2022-04-06 Miltenyi Biotec B.V. & Co. KG Bright fluorochromes based on multimerization of fluorescent dyes on branched polyether scaffolds
JP2017002000A (en) * 2015-06-12 2017-01-05 キヤノン株式会社 Compound or its pharmaceutically acceptable salt, and contrast medium for photoacoustic imaging having the compound or its pharmaceutically acceptable salt
GB201516987D0 (en) 2015-09-25 2015-11-11 Illumina Cambridge Ltd Polymethine compounds and their use as fluorescent labels
CN105254655B (en) * 2015-11-20 2017-03-22 江汉大学 Fluorescent amino acid based on BODIPY as well as synthetic method and application thereof
WO2017110387A1 (en) * 2015-12-25 2017-06-29 富士フイルム株式会社 Coloring composition, ink for inkjet, printing method, and compound
US9862682B2 (en) 2016-01-08 2018-01-09 BroadPharm Functionalized pegylated cyanine compounds, pharmaceutical compositions, and methods of use thereof
US9850383B2 (en) * 2016-01-27 2017-12-26 Gene Tools, Llc Uncharged pyrenyloxy sulfonamide dyes for conjugation with biomolecules
CN105688230B (en) * 2016-02-02 2018-12-11 史春梦 Seven methine indoles cyanine dyes-polyethylene glycol-folic acid composite and preparation method and application
EP3436820A2 (en) * 2016-03-30 2019-02-06 Qualizyme Diagnostics GmbH&Co KG Detecting microbial infection in wounds
CN109073556A (en) * 2016-04-28 2018-12-21 国立大学法人名古屋大学 Fluorescent probe, fluorescent detection method and method of using fluorescent probe
CN109844538A (en) * 2016-09-01 2019-06-04 生命科技股份有限公司 Compositions and methods for enhancing fluorescence
US11016092B2 (en) * 2017-03-23 2021-05-25 Abbott Laboratories Methods for aiding in the diagnosis and determination of the extent of traumatic brain injury in a human subject using the early biomarker ubiquitin carboxy-terminal hydrolase L1
US10877048B2 (en) * 2017-04-15 2020-12-29 Abbott Laboratories Methods for aiding in the hyperacute diagnosis and determination of traumatic brain injury in a human subject using early biomarkers
WO2018234557A1 (en) * 2017-06-23 2018-12-27 Nanotemper Technologies Gmbh Methods for measuring inter- and/or intra-molecular interactions
US11549017B2 (en) * 2017-09-29 2023-01-10 Perkinelmer Health Sciences, Inc. NIR to SWIR fluorescent compounds for imaging and detection
CN108458964A (en) * 2017-10-31 2018-08-28 天津协和华美医学诊断技术有限公司 A kind of antibody compositions and its application in the detection of lymphoma lymphoplasmacytic minimal residual
CN108017928A (en) * 2017-12-15 2018-05-11 淮海工学院 A kind of Pegylation indole cyanine dyes for biomolecular labeling and preparation method thereof
CN109054013A (en) * 2018-08-22 2018-12-21 东北大学 A kind of modified indocyanine green and preparation method thereof
DE102018124199B4 (en) 2018-10-01 2023-09-07 Abberior GmbH New fluorescent dyes, their synthesis and new analysis methods for glycan analysis
CN112147113B (en) * 2019-06-26 2023-04-07 西南交通大学 Application of coumarin derivative in quantitative detection of sulfur dioxide
CN112442020B (en) * 2019-09-03 2022-06-14 南京大学 Fluorescent probe capable of tracing mitochondria-lysosome interaction in super-resolution mode
JP7359869B2 (en) * 2019-12-19 2023-10-11 富士フイルム株式会社 Compounds and fluorescently labeled biological substances using them
JP7479667B2 (en) * 2020-02-12 2024-05-09 国立大学法人山形大学 Compound having a sulfonylaniline skeleton or a salt thereof, or organic fluorescent material having the same
CN111073884A (en) * 2020-02-14 2020-04-28 昆明理工大学 A method for improving the detection accuracy of SNP loci with functional effects in SNPs with a distance of less than 50 bp in the non-coding region
CN113736285A (en) * 2020-03-18 2021-12-03 宝天生物科技（上海）有限公司 Ultraviolet light excited fluorescent dye and application thereof
EP4140999A4 (en) * 2020-04-24 2023-11-29 FUJIFILM Corporation Compound and labeled biomaterial in which same is used
CN116134044A (en) * 2020-07-30 2023-05-16 富士胶片株式会社 Compounds and labeled biological substances using the same
JP2022053282A (en) * 2020-09-24 2022-04-05 大塚製薬株式会社 Cyanine dye, labeling reagent and detection method
JP7660821B2 (en) * 2021-04-16 2025-04-14 株式会社ニコン Compound, mitochondrial staining agent, and method for fluorescent staining of mitochondria
JPWO2022265043A1 (en) 2021-06-18 2022-12-22
CN117642469A (en) * 2021-07-13 2024-03-01 太阳控股株式会社 Xanthene compound and process for producing the same
WO2023004356A2 (en) * 2021-07-21 2023-01-26 Life Technologies Corporation Azaindole cyanine dyes, uses, and methods of preparation
JPWO2023022187A1 (en) * 2021-08-18 2023-02-23
CN114062676B (en) * 2021-10-19 2024-04-16 杭州迪英加科技有限公司 Antibody reagent buffer solution for anti-counterfeiting and application thereof
CN114214061B (en) * 2022-02-22 2022-05-17 北京大学 Polyethylene glycol modified zwitterionic fluorescent probe, preparation and application thereof
CN114621248B (en) * 2022-03-11 2023-11-17 华南理工大学 Fluorescent probe for recognizing RNA and having photodynamic and preparation method thereof
CN115372341B (en) * 2022-05-14 2025-04-25 西北工业大学深圳研究院 Bioluminescent detection system of bacterial cell wall nascent peptidoglycan constructed by D-lysine derivatives and its application method
CN121100114A (en) * 2023-05-22 2025-12-09 深圳博芮健制药有限公司 Sulfur-containing fused-ring compounds, pharmaceutical compositions containing them, their preparation methods and applications
CN117890582B (en) * 2023-12-21 2025-01-21 南京迪安医学检验所有限公司 A herpes simplex virus detection kit and detection method thereof
WO2025230756A1 (en) * 2024-05-01 2025-11-06 BioLegend, Inc. Compositions for use with multiple fluorophores and methods of using
CN119119042B (en) * 2024-09-14 2025-11-25 大连理工大学 Near-infrared responsive asymmetric polymethyl cyanine dyes, their preparation methods and applications

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4877411A (en) * 1988-08-29 1989-10-31 Milliken Research Corporation Method for color mounting the level of finish applied to textile materials and for color coding textile materials: amine-reducible fugitive tints
US5880287A (en) * 1990-05-15 1999-03-09 Hyperion, Inc. Polyoxyhydrocarbyl related products and methods for fluorescence assays
US20040152084A1 (en) * 2003-01-31 2004-08-05 Slattum Paul M. Compounds and processes for single-pot attachment of a label to nucleic acid
WO2004085539A2 (en) * 2003-03-21 2004-10-07 Amersham Biosciences Corp Cyanine dye labelling reagents with meso-substitution
EP1535969A2 (en) * 2003-11-26 2005-06-01 Dyomics GmbH Coumarin based new polymethine dyes with adjustable Stoke's-Shift
EP1619501A1 (en) * 2004-07-22 2006-01-25 Schering AG Use of cyanine dyes for the diagnosis of disease associated with angiogenesis
EP1792949A2 (en) * 2005-12-05 2007-06-06 Dyomics GmbH Hydrophilic marker on the basis of diastereomeric cyanines

Family Cites Families (70)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4711955A (en) 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
US4520110A (en) 1981-10-06 1985-05-28 The Board Of Trustees Of The Leland Stanford Junior University Fluorescent immunoassay employing a phycobiliprotein labeled ligand or receptor
US5268486A (en) * 1986-04-18 1993-12-07 Carnegie-Mellon Unversity Method for labeling and detecting materials employing arylsulfonate cyanine dyes
US5047519A (en) 1986-07-02 1991-09-10 E. I. Du Pont De Nemours And Company Alkynylamino-nucleotides
US4813973A (en) * 1988-01-11 1989-03-21 The University Of Toronto Innovations Foundation Modified, water-soluble polymers containing amide groups reacted with xanthydrol and other dyes
DE3912046B4 (en) * 1988-09-02 2004-03-25 Carnegie Mellon University Method of labeling a component of an aqueous liquid and luminescent photostable reaction product
US5082934A (en) 1989-04-05 1992-01-21 Naxcor Coumarin derivatives for use as nucleotide crosslinking reagents
US5122614A (en) 1989-04-19 1992-06-16 Enzon, Inc. Active carbonates of polyalkylene oxides for modification of polypeptides
US5324844A (en) 1989-04-19 1994-06-28 Enzon, Inc. Active carbonates of polyalkylene oxides for modification of polypeptides
US5312808A (en) 1989-11-22 1994-05-17 Enzon, Inc. Fractionation of polyalkylene oxide-conjugated hemoglobin solutions
US5234903A (en) 1989-11-22 1993-08-10 Enzon, Inc. Chemically modified hemoglobin as an effective, stable non-immunogenic red blood cell substitute
US5650388A (en) 1989-11-22 1997-07-22 Enzon, Inc. Fractionated polyalkylene oxide-conjugated hemoglobin solutions
US5298643A (en) 1992-12-22 1994-03-29 Enzon, Inc. Aryl imidate activated polyalkylene oxides
US5349001A (en) 1993-01-19 1994-09-20 Enzon, Inc. Cyclic imide thione activated polyalkylene oxides
US5808044A (en) * 1993-01-22 1998-09-15 Pharmacia Biotech Inc. Indocarbocyanine and benzindocarbocyanine phosphoramidites
US5321095A (en) 1993-02-02 1994-06-14 Enzon, Inc. Azlactone activated polyalkylene oxides
US5585235A (en) 1993-04-13 1996-12-17 Diagnescent Technologies, Inc. Fluorescent assay and method that corrects for spectral interference
US5436134A (en) 1993-04-13 1995-07-25 Molecular Probes, Inc. Cyclic-substituted unsymmetrical cyanine dyes
US5880131A (en) 1993-10-20 1999-03-09 Enzon, Inc. High molecular weight polymer-based prodrugs
US5605976A (en) 1995-05-15 1997-02-25 Enzon, Inc. Method of preparing polyalkylene oxide carboxylic acids
WO1995033490A1 (en) 1994-06-02 1995-12-14 Enzon, Inc. Method of solubilizing substantially water insoluble materials
US5777079A (en) 1994-11-10 1998-07-07 The Regents Of The University Of California Modified green fluorescent proteins
US5625048A (en) 1994-11-10 1997-04-29 The Regents Of The University Of California Modified green fluorescent proteins
DE4445065A1 (en) 1994-12-07 1996-06-13 Diagnostikforschung Inst Methods for in-vivo diagnostics using NIR radiation
JP3669752B2 (en) * 1995-01-30 2005-07-13 第一化学薬品株式会社 Diagnostic marker
KR100397020B1 (en) * 1995-01-30 2004-04-29 다이이치 가가쿠 야쿠힝 가부시키가이샤 Diagnostic marker
US5756593A (en) 1995-05-15 1998-05-26 Enzon, Inc. Method of preparing polyalkyene oxide carboxylic acids
US5986093A (en) 1995-06-07 1999-11-16 Carnegie Mellon University Monomethine cyanines rigidized by a two-carbon chain
US6008373A (en) 1995-06-07 1999-12-28 Carnegie Mellon University Fluorescent labeling complexes with large stokes shift formed by coupling together cyanine and other fluorochromes capable of resonance energy transfer
AU6113396A (en) 1995-06-14 1997-01-15 Regents Of The University Of California, The Novel high affinity human antibodies to tumor antigens
US5723343A (en) 1995-08-28 1998-03-03 University Of Florida Autoantibodies in patients with acquired hypoparathyroidism and assay method therefor
US6766183B2 (en) * 1995-11-22 2004-07-20 Medtronic Minimed, Inc. Long wave fluorophore sensor compounds and other fluorescent sensor compounds in polymers
US5714386A (en) 1996-01-11 1998-02-03 Board Of Trustees Of The Leland Stanford Junior University Cy7-allophycocyanin conjugates for use in multiplex fluorescence detection assays
US6124128A (en) 1996-08-16 2000-09-26 The Regents Of The University Of California Long wavelength engineered fluorescent proteins
JP3601972B2 (en) * 1998-05-14 2004-12-15 松下電器産業株式会社 Dye-labeled protein complex and method for producing the same
US5696157A (en) 1996-11-15 1997-12-09 Molecular Probes, Inc. Sulfonated derivatives of 7-aminocoumarin
US6037137A (en) * 1997-02-20 2000-03-14 Oncoimmunin, Inc. Fluorogenic peptides for the detection of protease activity
US6046925A (en) 1997-04-14 2000-04-04 The Regents Of The University Of California Photochromic fluorescent proteins and optical memory storage devices based on fluorescent proteins
WO1998049198A1 (en) 1997-04-30 1998-11-05 Enzon, Inc. Single-chain antigen-binding proteins capable of glycosylation, production and uses thereof
GB9712524D0 (en) * 1997-06-16 1997-08-20 Nycomed Imaging As Method
US5866366A (en) 1997-07-01 1999-02-02 Smithkline Beecham Corporation gidB
US6130101A (en) * 1997-09-23 2000-10-10 Molecular Probes, Inc. Sulfonated xanthene derivatives
US6133445A (en) 1997-12-17 2000-10-17 Carnegie Mellon University Rigidized trimethine cyanine dyes
US5931758A (en) * 1998-04-08 1999-08-03 General Dynamics Land Systems, Inc. Simplified multi-range hydromechanical transmission for vehicles
US6251382B1 (en) 1998-04-17 2001-06-26 Enzon, Inc. Biodegradable high molecular weight polymeric linkers and their conjugates
US6485703B1 (en) * 1998-07-31 2002-11-26 The Texas A&M University System Compositions and methods for analyte detection
GB2341189B (en) 1998-08-31 2001-06-13 Amersham Pharm Biotech Inc Energy transfer dyes
DE60001531T2 (en) 1999-04-23 2003-10-02 Molecular Probes, Inc. XANTHENE DYES AND THEIR USE AS LUMINESCENT EXTINGUISHING COMPOUNDS
WO2001016375A2 (en) 1999-08-30 2001-03-08 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services High speed parallel molecular nucleic acid sequencing
EP2045252B1 (en) 2000-08-04 2013-05-01 Life Technologies Corporation Derivatives of 1,2-dihydro-7-hydroxyquinolines containing fused rings
DE10046215B4 (en) 2000-09-19 2004-04-15 Institut für Chemo- und Biosensorik Münster e.V. i.Ins. Fluorochromes and their use
AU2001294859A1 (en) 2000-09-29 2002-04-08 Molecular Probes, Inc. Modified carbocyanine dyes and their conjugates
US6387672B1 (en) 2000-12-04 2002-05-14 Beckman Coulter, Inc. Photo-induced electron transfer fluorescent sensor molecules
US20030044353A1 (en) * 2001-01-05 2003-03-06 Ralph Weissleder Activatable imaging probes
US6632944B2 (en) * 2001-06-22 2003-10-14 Cytec Technology Corp. Process for isolation of monophenolic-bisaryl triazines
EP1273584B1 (en) 2001-07-03 2004-02-18 Dyomics GmbH 2- or 4-Chromenyliden based merocyanines and use thereof
JP4318296B2 (en) 2001-08-29 2009-08-19 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション Labeled nucleoside polyphosphate
US20050069962A1 (en) 2001-10-12 2005-03-31 Archer Robert M Antibody complexes and methods for immunolabeling
DE10160524A1 (en) 2001-12-05 2003-06-26 Dyomics Gmbh New fluorescent markers with a large Stokes shift
US20030134790A1 (en) * 2002-01-11 2003-07-17 University Of Medicine And Dentistry Of New Jersey Bone Morphogenetic Protein-2 And Bone Morphogenetic Protein-4 In The Treatment And Diagnosis Of Cancer
EP1546673A4 (en) * 2002-09-23 2006-08-09 Chromagen Inc Novel green and orange fluorescent labels and their uses
US7563907B2 (en) 2002-12-05 2009-07-21 Dyomics Gmbh Compounds based on polymethines
US7456277B2 (en) 2004-08-09 2008-11-25 Silverbrook Research Pty Ltd Method of minimizing absorption of visible light in ink compositions comprising IR-absorbing metal-cyanine dyes
WO2006079372A1 (en) * 2005-01-31 2006-08-03 Ablynx N.V. Method for generating variable domain sequences of heavy chain antibodies
AU2006249340A1 (en) 2005-05-26 2006-11-30 Ensemble Discovery Corportion Biodetection by nucleic acid-templated chemistry
US7561888B2 (en) 2005-07-15 2009-07-14 Cisco Technology, Inc. Efficiently bounding the location of a mobile communications device
DE102006029454A1 (en) * 2005-12-05 2007-06-06 Dyomics Gmbh Hydrophilic markers based on diastereomeric
US8586743B2 (en) * 2007-01-30 2013-11-19 Life Technologies Corporation Labeling reagents and methods of their use
WO2008093647A1 (en) 2007-01-31 2008-08-07 Tohoku University Microarray, method for production thereof, and method for detection of interaction between organic molecule and active substance
CN101946171B (en) 2007-12-14 2014-03-12 拜奥蒂乌姆股份有限公司 Fluorescent compounds

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4877411A (en) * 1988-08-29 1989-10-31 Milliken Research Corporation Method for color mounting the level of finish applied to textile materials and for color coding textile materials: amine-reducible fugitive tints
US5880287A (en) * 1990-05-15 1999-03-09 Hyperion, Inc. Polyoxyhydrocarbyl related products and methods for fluorescence assays
US20040152084A1 (en) * 2003-01-31 2004-08-05 Slattum Paul M. Compounds and processes for single-pot attachment of a label to nucleic acid
WO2004085539A2 (en) * 2003-03-21 2004-10-07 Amersham Biosciences Corp Cyanine dye labelling reagents with meso-substitution
EP1535969A2 (en) * 2003-11-26 2005-06-01 Dyomics GmbH Coumarin based new polymethine dyes with adjustable Stoke's-Shift
EP1619501A1 (en) * 2004-07-22 2006-01-25 Schering AG Use of cyanine dyes for the diagnosis of disease associated with angiogenesis
EP1792949A2 (en) * 2005-12-05 2007-06-06 Dyomics GmbH Hydrophilic marker on the basis of diastereomeric cyanines

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
C.HÖLTKE ET AL.: "A Fluorescent Photoprobe for the Imaging of Endothelin Receptors", BIOCONJUGATE CHEM., vol. 18, 2007, pages 685 - 694, XP002629913 *
K.LICHA ET AL.: "Synthesis and Characterization of Cyanine Dye-Poly(ethylene Glycol) Conjugates as contrast Agents for in vivo Fluorescent Imaging", PROC.SPIE, vol. 3196, 1998, pages 98 - 102, XP002629912 *
N.NARAYANAN ET AL.: "A new Method for the Synthesis of Heptamethine Cyanine Dyes: Synthesis of new Near-Infrared Fluorescent Labels", J.ORG.CHEM., vol. 60, 1995, pages 2391 - 2395, XP002629916 *
See also references of WO2009078970A1 *
T.TOKIMOTO ET AL.: "Probing Orientations of single Fluorescent Labels on a Peptide Reversible Binding to the Human Delta-Opioide Receptor", APPL.SPECTRSCOPY, vol. 61, no. 2, 2007, pages 130 - 137, XP002629914 *
W.K.MOON ET AL.: "Enhanced Tumor Detection using a Folate Receptor-Targeted Near-Infrared Fluorochrome Conjugate", BIOCONJUGATE CEHM,., vol. 14, 2003, pages 539 - 545, XP002629915 *

Also Published As

Publication number Publication date
EP2223086B2 (en) 2016-06-01
US9097667B2 (en) 2015-08-04
US9791450B2 (en) 2017-10-17
US20160033520A1 (en) 2016-02-04
EP2223086A1 (en) 2010-09-01
EP2223086B1 (en) 2013-03-20
US20090305410A1 (en) 2009-12-10
US20180156810A1 (en) 2018-06-07
JP2015172199A (en) 2015-10-01
WO2009078970A1 (en) 2009-06-25
CN101946171B (en) 2014-03-12
JP2011506673A (en) 2011-03-03
CN101946171A (en) 2011-01-12

Similar Documents

Publication Publication Date Title
EP2223086A4 (en) FLUORESCENT COMPOUNDS
EP2044055A4 (en) AMIDED COMPOUNDS
EP2132082A4 (en) NEW MOLYBDENUM COMPOUNDS
EP2211620A4 (en) THERAPEUTIC COMPOUNDS
EP2320907A4 (en) THERAPEUTIC COMPOUNDS
EP2302662A4 (en) FLUORESCENT LAMP
EP2163502A4 (en) ELEVATOR
EP2268285A4 (en) THERAPEUTIC COMPOUNDS
EP2165960A4 (en) ELEVATOR
EP2168901A4 (en) ELEVATOR
EP2135921A4 (en) FLUORESCENT SUBSTANCE M-C-N-O
EP2035369A4 (en) THERAPEUTIC COMPOUNDS
EP2120578A4 (en) KINASE INHIBITOR COMPOUNDS
EP2088861A4 (en) BENZIMIDAZOLE COMPOUNDS
FI20070694A0 (en) Elevator arrangement
EP2379510A4 (en) QUINAZOLINONE COMPOUNDS
EP2321275A4 (en) ANTIMALARIAL COMPOUNDS
EP2164324A4 (en) THERAPEUTIC COMPOUNDS
EP2112114A4 (en) ELEVATOR
EP2117306A4 (en) NEUROGENIC COMPOUNDS
EP2268635A4 (en) HETEROCYCLIC COMPOUNDS
EP2215184A4 (en) FLUORESCENT ORGANIC COMPOSITIONS
FI20070562L (en) Elevator
EP2212305A4 (en) BETA-LACTONE COMPOUNDS
EP2204371A4 (en) FLUORESCENT MOLECULAR

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20100702

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MT NL NO PL PT RO SE SI SK TR

AX Request for extension of the european patent

Extension state: AL BA MK RS

DAX Request for extension of the european patent (deleted)
A4 Supplementary search report drawn up and despatched

Effective date: 20110405

RIC1 Information provided on ipc code assigned before grant

Ipc: C09B 69/00 20060101ALI20110325BHEP

Ipc: G01N 21/64 20060101AFI20090710BHEP

17Q First examination report despatched

Effective date: 20120206

RIC1 Information provided on ipc code assigned before grant

Ipc: C09B 23/08 20060101ALI20120718BHEP

Ipc: C09B 57/02 20060101ALI20120718BHEP

Ipc: C09B 69/00 20060101ALI20120718BHEP

Ipc: G01N 21/64 20060101AFI20120718BHEP

GRAP Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOSNIGR1

GRAS Grant fee paid

Free format text: ORIGINAL CODE: EPIDOSNIGR3

GRAA (expected) grant

Free format text: ORIGINAL CODE: 0009210

AK Designated contracting states

Kind code of ref document: B1

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MT NL NO PL PT RO SE SI SK TR

REG Reference to a national code

Ref country code: GB

Ref legal event code: FG4D

REG Reference to a national code

Ref country code: CH

Ref legal event code: EP

REG Reference to a national code

Ref country code: IE

Ref legal event code: FG4D

REG Reference to a national code

Ref country code: AT

Ref legal event code: REF

Ref document number: 602353

Country of ref document: AT

Kind code of ref document: T

Effective date: 20130415

REG Reference to a national code

Ref country code: DE

Ref legal event code: R096

Ref document number: 602008023122

Country of ref document: DE

Effective date: 20130516

REG Reference to a national code

Ref country code: SE

Ref legal event code: TRGR

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: LT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130320

Ref country code: BG

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130620

Ref country code: NO

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130620

Ref country code: ES

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130701

REG Reference to a national code

Ref country code: NL

Ref legal event code: T3

REG Reference to a national code

Ref country code: AT

Ref legal event code: MK05

Ref document number: 602353

Country of ref document: AT

Kind code of ref document: T

Effective date: 20130320

REG Reference to a national code

Ref country code: LT

Ref legal event code: MG4D

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: FI

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130320

Ref country code: LV

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130320

Ref country code: SI

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130320

Ref country code: GR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130621

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: HR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130320

Ref country code: BE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130320

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: PT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130722

Ref country code: SK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130320

Ref country code: IS

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130720

Ref country code: RO

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130320

Ref country code: AT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130320

Ref country code: EE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130320

Ref country code: CZ

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130320

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: CY

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130320

Ref country code: PL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130320

PLBI Opposition filed

Free format text: ORIGINAL CODE: 0009260

PLAX Notice of opposition and request to file observation + time limit sent

Free format text: ORIGINAL CODE: EPIDOSNOBS2

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: DK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130320

26 Opposition filed

Opponent name: MILTENYI BIOTEC GMBH

Effective date: 20131219

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: IT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130320

REG Reference to a national code

Ref country code: DE

Ref legal event code: R026

Ref document number: 602008023122

Country of ref document: DE

Effective date: 20131219

PLAF Information modified related to communication of a notice of opposition and request to file observations + time limit

Free format text: ORIGINAL CODE: EPIDOSCOBS2

REG Reference to a national code

Ref country code: NL

Ref legal event code: V1

Effective date: 20140701

REG Reference to a national code

Ref country code: SE

Ref legal event code: EUG

REG Reference to a national code

Ref country code: CH

Ref legal event code: PL

PLBB Reply of patent proprietor to notice(s) of opposition received

Free format text: ORIGINAL CODE: EPIDOSNOBS3

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: LU

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20131212

Ref country code: MC

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130320

Ref country code: SE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20131213

REG Reference to a national code

Ref country code: IE

Ref legal event code: MM4A

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: CH

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20131231

Ref country code: NL

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20140701

Ref country code: IE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20131212

Ref country code: LI

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20131231

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: TR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130320

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: HU

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT; INVALID AB INITIO

Effective date: 20081212

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: MT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130320

REG Reference to a national code

Ref country code: FR

Ref legal event code: PLFP

Year of fee payment: 8

PUAH Patent maintained in amended form

Free format text: ORIGINAL CODE: 0009272

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: PATENT MAINTAINED AS AMENDED

27A Patent maintained in amended form

Effective date: 20160601

AK Designated contracting states

Kind code of ref document: B2

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MT NL NO PL PT RO SE SI SK TR

REG Reference to a national code

Ref country code: DE

Ref legal event code: R102

Ref document number: 602008023122

Country of ref document: DE

REG Reference to a national code

Ref country code: FR

Ref legal event code: PLFP

Year of fee payment: 9

REG Reference to a national code

Ref country code: FR

Ref legal event code: PLFP

Year of fee payment: 10

P01 Opt-out of the competence of the unified patent court (upc) registered

Effective date: 20230515

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: GB

Payment date: 20241227

Year of fee payment: 17

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: FR

Payment date: 20241226

Year of fee payment: 17

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: DE

Payment date: 20241227

Year of fee payment: 17