EP2167532A4 - Plasmid-encoded neurotoxin genes in clostridium botulinum serotype a subtypes - Google Patents

Plasmid-encoded neurotoxin genes in clostridium botulinum serotype a subtypes

Info

Publication number
EP2167532A4
EP2167532A4 EP08772138A EP08772138A EP2167532A4 EP 2167532 A4 EP2167532 A4 EP 2167532A4 EP 08772138 A EP08772138 A EP 08772138A EP 08772138 A EP08772138 A EP 08772138A EP 2167532 A4 EP2167532 A4 EP 2167532A4
Authority
EP
European Patent Office
Prior art keywords
subtypes
plasmid
clostridium botulinum
botulinum serotype
encoded
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP08772138A
Other languages
German (de)
French (fr)
Other versions
EP2167532A1 (en
Inventor
Eric A Johnson
Marite Bradshaw
Kristin Marshall
Sabine Pellett
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wisconsin Alumni Research Foundation
Original Assignee
Wisconsin Alumni Research Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wisconsin Alumni Research Foundation filed Critical Wisconsin Alumni Research Foundation
Publication of EP2167532A1 publication Critical patent/EP2167532A1/en
Publication of EP2167532A4 publication Critical patent/EP2167532A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24—Metalloendopeptidases (3.4.24)
    • C12Y304/24069—Bontoxilysin (3.4.24.69), i.e. botulinum neurotoxin
EP08772138A 2007-06-29 2008-06-27 Plasmid-encoded neurotoxin genes in clostridium botulinum serotype a subtypes Withdrawn EP2167532A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US94709007P 2007-06-29 2007-06-29
PCT/US2008/068532 WO2009006281A1 (en) 2007-06-29 2008-06-27 Plasmid-encoded neurotoxin genes in clostridium botulinum serotype a subtypes

Publications (2)

Publication Number Publication Date
EP2167532A1 EP2167532A1 (en) 2010-03-31
EP2167532A4 true EP2167532A4 (en) 2010-07-14

Family

ID=40226483

Family Applications (1)

Application Number Title Priority Date Filing Date
EP08772138A Withdrawn EP2167532A4 (en) 2007-06-29 2008-06-27 Plasmid-encoded neurotoxin genes in clostridium botulinum serotype a subtypes

Country Status (8)

Country Link
US (3) US8435759B2 (en)
EP (1) EP2167532A4 (en)
CN (1) CN101848930A (en)
AU (1) AU2008270020B2 (en)
BR (1) BRPI0813115A2 (en)
CA (1) CA2691825C (en)
NZ (1) NZ582087A (en)
WO (1) WO2009006281A1 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009006281A1 (en) 2007-06-29 2009-01-08 Wisconsin Alumni Research Foundation Plasmid-encoded neurotoxin genes in clostridium botulinum serotype a subtypes
WO2011047274A1 (en) 2009-10-15 2011-04-21 Wisconsin Alumni Research Foundation Conjugative plasmids from clostridium botulinum and methods of use thereof
WO2013049139A1 (en) * 2011-09-28 2013-04-04 Wisconsin Alumni Research Foundation Purification, characterization, and use of clostridium botulinum neurotoxin bont/a3
CN103107859B (en) * 2011-11-15 2015-08-19 中国科学院大学 Based on combined signal source and the information security coding and decoding method of CCSDS IDC and SMS4
WO2018081282A1 (en) * 2016-10-25 2018-05-03 Cellsnap Llc Neurotoxins and uses thereof
US10688162B2 (en) * 2017-01-10 2020-06-23 Cellsnap Llc Hybrid neurotoxins and uses thereof
WO2019075182A1 (en) * 2017-10-13 2019-04-18 Wisconsin Alumni Research Foundation Botulinum neurotoxin a subtype 6 and pharmacological methods of use
CN109022603A (en) * 2018-07-09 2018-12-18 中国疾病预防控制中心传染病预防控制所 Detect the real-time fluorescence quantitative PCR nucleic acid sequence and kit of Type B clostridium botulinum

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5955368A (en) * 1998-04-06 1999-09-21 Wisconsin Alumni Research Foundation Expression system for clostridium species
WO2009006281A1 (en) 2007-06-29 2009-01-08 Wisconsin Alumni Research Foundation Plasmid-encoded neurotoxin genes in clostridium botulinum serotype a subtypes

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ARNDT J W ET AL: "A Structural Perspective of the Sequence Variability Within Botulinum Neurotoxin Subtypes A1-A4", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB LNKD- DOI:10.1016/J.JMB.2006.07.040, vol. 362, no. 4, 29 September 2006 (2006-09-29), pages 733 - 742, XP024951406, ISSN: 0022-2836, [retrieved on 20060929] *
MARSHALL ET AL: "Plasmid encoded neurotoxin genes in Clostridium botulinum serotype A subtypes", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US LNKD- DOI:10.1016/J.BBRC.2007.06.166, vol. 361, no. 1, 29 July 2007 (2007-07-29), pages 49 - 54, XP022176145, ISSN: 0006-291X *
See also references of WO2009006281A1 *
WEICKERT M J ET AL: "PRODUCTION OF TOXIN BY CLOSTRIDIUM-BOTULINUM TYPE A STRAINS CURED OF PLASMIDS", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 51, no. 1, 1986, pages 52 - 56, XP002584142, ISSN: 0099-2240 *
ZHOU Y ET AL: "The genes for the Clostridium botulinum type G toxin complex are on a plasmid.", May 1995, INFECTION AND IMMUNITY MAY 1995 LNKD- PUBMED:7729925, VOL. 63, NR. 5, PAGE(S) 2087 - 2091, ISSN: 0019-9567, XP002584143 *

Also Published As

Publication number Publication date
CA2691825C (en) 2016-05-17
AU2008270020A1 (en) 2009-01-08
WO2009006281A1 (en) 2009-01-08
US8435759B2 (en) 2013-05-07
CA2691825A1 (en) 2009-01-08
US9453057B2 (en) 2016-09-27
US20100311118A1 (en) 2010-12-09
EP2167532A1 (en) 2010-03-31
CN101848930A (en) 2010-09-29
BRPI0813115A2 (en) 2017-05-23
NZ582087A (en) 2012-06-29
US20140065668A1 (en) 2014-03-06
AU2008270020B2 (en) 2013-05-16
US20160362671A1 (en) 2016-12-15

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