EP2121941A4 - Tobacco plants having reduced nicotine demethylase activity - Google Patents

Tobacco plants having reduced nicotine demethylase activity

Info

Publication number
EP2121941A4
EP2121941A4 EP07865628A EP07865628A EP2121941A4 EP 2121941 A4 EP2121941 A4 EP 2121941A4 EP 07865628 A EP07865628 A EP 07865628A EP 07865628 A EP07865628 A EP 07865628A EP 2121941 A4 EP2121941 A4 EP 2121941A4
Authority
EP
European Patent Office
Prior art keywords
tobacco plants
nicotine demethylase
demethylase activity
reduced nicotine
reduced
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP07865628A
Other languages
German (de)
French (fr)
Other versions
EP2121941B1 (en
EP2121941A2 (en
Inventor
Dongmei Xu
Mark T Nielsen
Yanxin Shen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
US Smokeless Tobacco Co LLC
Original Assignee
US Smokeless Tobacco Co LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=39536979&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=EP2121941(A4) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by US Smokeless Tobacco Co LLC filed Critical US Smokeless Tobacco Co LLC
Priority to EP12175334.7A priority Critical patent/EP2537939B1/en
Priority to EP10015540.7A priority patent/EP2333081B1/en
Priority to EP12175337.0A priority patent/EP2540830A3/en
Priority to EP17186176.8A priority patent/EP3301180A1/en
Publication of EP2121941A2 publication Critical patent/EP2121941A2/en
Publication of EP2121941A4 publication Critical patent/EP2121941A4/en
Application granted granted Critical
Publication of EP2121941B1 publication Critical patent/EP2121941B1/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0073—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
EP07865628.7A 2006-12-15 2007-12-13 Tobacco plants having reduced nicotine demethylase activity Active EP2121941B1 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP12175334.7A EP2537939B1 (en) 2006-12-15 2007-12-13 Tobacco plants having reduced nicotine demethylase activity
EP10015540.7A EP2333081B1 (en) 2006-12-15 2007-12-13 Tabacco plants having reduced nicotine demethylase activity
EP12175337.0A EP2540830A3 (en) 2006-12-15 2007-12-13 Tobacco plants having reduced nicotine demethylase activity
EP17186176.8A EP3301180A1 (en) 2006-12-15 2007-12-13 Tobacco plants having reduced nicotine demethylase activity

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11/611,782 US20070199097A1 (en) 2004-09-03 2006-12-15 Tobacco plants having a mutation in a nicotine demethylase gene
PCT/US2007/087386 WO2008076802A2 (en) 2006-12-15 2007-12-13 Tobacco plants having reduced nicotine demethylase activity

Related Child Applications (6)

Application Number Title Priority Date Filing Date
EP12175337.0A Division-Into EP2540830A3 (en) 2006-12-15 2007-12-13 Tobacco plants having reduced nicotine demethylase activity
EP10015540.7A Division EP2333081B1 (en) 2006-12-15 2007-12-13 Tabacco plants having reduced nicotine demethylase activity
EP10015540.7A Division-Into EP2333081B1 (en) 2006-12-15 2007-12-13 Tabacco plants having reduced nicotine demethylase activity
EP12175334.7A Division-Into EP2537939B1 (en) 2006-12-15 2007-12-13 Tobacco plants having reduced nicotine demethylase activity
EP12175334.7A Division EP2537939B1 (en) 2006-12-15 2007-12-13 Tobacco plants having reduced nicotine demethylase activity
EP17186176.8A Division EP3301180A1 (en) 2006-12-15 2007-12-13 Tobacco plants having reduced nicotine demethylase activity

Publications (3)

Publication Number Publication Date
EP2121941A2 EP2121941A2 (en) 2009-11-25
EP2121941A4 true EP2121941A4 (en) 2010-03-31
EP2121941B1 EP2121941B1 (en) 2015-10-28

Family

ID=39536979

Family Applications (5)

Application Number Title Priority Date Filing Date
EP12175337.0A Withdrawn EP2540830A3 (en) 2006-12-15 2007-12-13 Tobacco plants having reduced nicotine demethylase activity
EP17186176.8A Pending EP3301180A1 (en) 2006-12-15 2007-12-13 Tobacco plants having reduced nicotine demethylase activity
EP12175334.7A Active EP2537939B1 (en) 2006-12-15 2007-12-13 Tobacco plants having reduced nicotine demethylase activity
EP07865628.7A Active EP2121941B1 (en) 2006-12-15 2007-12-13 Tobacco plants having reduced nicotine demethylase activity
EP10015540.7A Active EP2333081B1 (en) 2006-12-15 2007-12-13 Tabacco plants having reduced nicotine demethylase activity

Family Applications Before (3)

Application Number Title Priority Date Filing Date
EP12175337.0A Withdrawn EP2540830A3 (en) 2006-12-15 2007-12-13 Tobacco plants having reduced nicotine demethylase activity
EP17186176.8A Pending EP3301180A1 (en) 2006-12-15 2007-12-13 Tobacco plants having reduced nicotine demethylase activity
EP12175334.7A Active EP2537939B1 (en) 2006-12-15 2007-12-13 Tobacco plants having reduced nicotine demethylase activity

Family Applications After (1)

Application Number Title Priority Date Filing Date
EP10015540.7A Active EP2333081B1 (en) 2006-12-15 2007-12-13 Tabacco plants having reduced nicotine demethylase activity

Country Status (10)

Country Link
US (3) US20070199097A1 (en)
EP (5) EP2540830A3 (en)
JP (2) JP5441708B2 (en)
CN (2) CN103993023B (en)
AP (1) AP2009004905A0 (en)
AR (1) AR064392A1 (en)
BR (1) BRPI0719984A2 (en)
HK (1) HK1156352A1 (en)
TW (1) TW200845887A (en)
WO (1) WO2008076802A2 (en)

Families Citing this family (48)

* Cited by examiner, † Cited by third party
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US7812227B2 (en) 2001-11-13 2010-10-12 U.S. Smokeless Tobacco Company Cloning of cytochrome p450 genes from nicotiana
US7700834B2 (en) * 2001-11-13 2010-04-20 U.S. Smokless Tobacco Company Nicotiana nucleic acid molecules and uses thereof
US8592663B2 (en) 2001-11-13 2013-11-26 U.S. Smokeless Tobacco Company Llc Tobacco nicotine demethylase genomic clone and uses thereof
US10266836B2 (en) 2001-11-13 2019-04-23 U.S. Smokeless Tobacco Company Llc Tobacco nicotine demethylase genomic clone and uses thereof
US8637731B2 (en) * 2003-10-16 2014-01-28 U.S. Smokeless Tobacco Company Nicotiana nucleic acid molecules and uses thereof
ZA200602938B (en) 2003-10-16 2008-06-25 Us Smokeless Tobacco Company Cloning of cytochrome p450 genes from Nicotiana
US8586837B2 (en) * 2004-04-29 2013-11-19 U.S. Smokeless Tobacco Company Llc Nicotiana nucleic acid molecules and uses thereof
US20070199097A1 (en) * 2004-09-03 2007-08-23 U.S. Smokeless Tobacco Company Tobacco plants having a mutation in a nicotine demethylase gene
WO2006091194A1 (en) * 2005-02-23 2006-08-31 North Carolina State University Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes
US9102948B2 (en) 2006-11-17 2015-08-11 22Nd Century Limited, Llc Regulating alkaloids
US8319011B2 (en) * 2006-12-15 2012-11-27 U.S. Smokeless Tobacco Company Llc Tobacco plants having reduced nicotine demethylase activity
US11332753B2 (en) 2006-12-15 2022-05-17 U.S. Smokeless Tobacco Company Llc Tobacco plants having reduced nicotine demethylase activity
TW200901904A (en) 2007-02-23 2009-01-16 Us Smokeless Tobacco Co Novel tobacco compositions and methods of making
US7667104B2 (en) * 2007-11-06 2010-02-23 Alliance One International, Inc. Tobacco cultivar AOB 171
US7667106B2 (en) * 2007-11-06 2010-02-23 Alliance One International, Inc. Tobacco cultivar ‘AOB 175’
US7665471B2 (en) * 2007-11-06 2010-02-23 Alliance One International, Inc. Tobacco cultivar AOB 171 and products therefrom
US7665472B2 (en) * 2007-11-06 2010-02-23 Alliance One International, Inc. Tobacco cultivar AOB 175 and products therefrom
EP2220231B1 (en) * 2007-11-12 2017-10-11 North Carolina State University Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes
CA2786813C (en) 2010-01-15 2023-03-28 North Carolina State University Compositions and methods for minimizing nornicotine synthesis in tobacco
CN102047844A (en) * 2010-12-13 2011-05-11 湖北省烟草科研所 Effective mutagenesis method for tobacco pollen
CN102181369B (en) * 2011-02-24 2012-10-10 中国科学院植物研究所 Genetic engineering chlamydomonas having increased hydrogen producing capacity and application thereof
WO2012118754A1 (en) 2011-02-28 2012-09-07 Altria Client Services Inc. Tobacco inbred plants albex1f and albex1ms
EP2680689A1 (en) * 2011-02-28 2014-01-08 North Carolina State University Tobacco inbred plants ncbex1f, ncbex1ms, and nc ex90
EP2565271A1 (en) * 2011-09-02 2013-03-06 Philip Morris Products S.A. Threonine synthase from Nicotiana tabacum and methods and uses thereof
CN102845476B (en) * 2012-09-04 2014-01-22 湖北中烟工业有限责任公司 Method for preparing inhibitor for reducing tobacco nicotine synthesis by using double-stranded RNA interference technology, and applications of inhibitor
US9521863B2 (en) 2012-09-19 2016-12-20 Altria Client Services Llc Methods of reducing tobacco-specific nitrosamines (TSNAs) and/or improving leaf quality in tobacco
CN105517430A (en) 2013-01-11 2016-04-20 北卡罗莱纳州立大学 Tobacco inbred plants K326 SRC, CMS K326 SRC, K346 SRC, CMS K346 SRC, NC1562-1 SRC, NCTG-61 SRC, CMS NCTG-61 SRC AND HYBRID NC196 SRC
US9560830B2 (en) 2013-03-05 2017-02-07 North Carolina State University Tobacco inbred and hybrid plants and uses thereof
CN111793640A (en) * 2013-12-06 2020-10-20 奥驰亚客户服务公司 Tobacco plants and methods of using such plants
US10036034B2 (en) 2014-02-13 2018-07-31 Nutech Ventures Sequences involved in plant yield and methods of using
WO2015134438A1 (en) 2014-03-03 2015-09-11 North Carolina State University Tobacco inbred and hybrid plants and tobacco products made thereof
EP3113604A1 (en) 2014-03-03 2017-01-11 North Carolina State University Tobacco inbred and hybrid plants and tobacco products made thereof
US9596823B2 (en) 2014-03-03 2017-03-21 North Carolina State University Tobacco inbred and hybrid plants and tobacco products made thereof
CA2959245A1 (en) * 2014-09-26 2016-03-31 Philip Morris Products S.A Reducing tobacco specific nitrosamines through alteration of the nitrate assimilation pathway
JP6702957B2 (en) * 2014-10-06 2020-06-03 アルトリア クライアント サービシーズ リミテッド ライアビリティ カンパニー Genetic control of axillary bud growth in tobacco plants
EP3313173A1 (en) * 2015-06-26 2018-05-02 Altria Client Services LLC Compositions and methods for producing tobacco plants and products having altered alkaloid levels
CN106879458A (en) * 2015-12-16 2017-06-23 中国农业科学院烟草研究所 A kind of method of EMS mutagenesis tobacco seed
CN108882689A (en) * 2016-03-30 2018-11-23 日本烟草产业株式会社 Tobacco plant body and preparation method thereof
CN106171967A (en) * 2016-07-13 2016-12-07 中国农业科学院烟草研究所 One grows tobacco Salanesol mutant method for creating and application
EP4342290A3 (en) 2016-10-07 2024-06-26 Altria Client Services LLC Tobacco plants having increased nitrogen efficiency and methods of using such plants
US20200140875A1 (en) * 2017-05-31 2020-05-07 22Nd Century Limited, Llc Genome editing methods for producing low-nicotine tobacco products
CN109307731B (en) * 2017-07-27 2024-01-26 红塔烟草(集团)有限责任公司 Tobacco leaf part discriminating method and device
WO2019140297A1 (en) * 2018-01-12 2019-07-18 Altria Client Services Llc Compositions and methods for producing tobacco plants and products having altered alkaloid levels
US11220695B2 (en) 2018-03-05 2022-01-11 Altria Client Services Llc Compositions and methods for producing tobacco plants and products having altered alkaloid levels with desirable leaf quality
US11536685B2 (en) 2020-02-06 2022-12-27 Trustees Of Boston University High throughput assay for identifying microbial redox enzymes
US11331020B2 (en) 2020-02-06 2022-05-17 Trustees Of Boston University Enzyme-based electrochemical nicotine biosensor
CN111662912A (en) * 2020-06-01 2020-09-15 云南省烟草农业科学研究院 Tobacco NtARF6 gene mutant and molecular identification method and application
CN116064567B (en) * 2022-07-26 2024-05-17 济南大学 Corn small kernel mutant and application thereof

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US20070199097A1 (en) * 2004-09-03 2007-08-23 U.S. Smokeless Tobacco Company Tobacco plants having a mutation in a nicotine demethylase gene

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WO2004035745A2 (en) * 2002-10-16 2004-04-29 U.S. Smokeless Tobacco Company Cloning of cytochrome p450 genes from nicotiana
US20070199097A1 (en) * 2004-09-03 2007-08-23 U.S. Smokeless Tobacco Company Tobacco plants having a mutation in a nicotine demethylase gene

Also Published As

Publication number Publication date
EP2540830A2 (en) 2013-01-02
JP2013172738A (en) 2013-09-05
WO2008076802A3 (en) 2008-11-13
EP2333081B1 (en) 2016-03-09
CN103993023A (en) 2014-08-20
EP2540830A3 (en) 2013-04-17
BRPI0719984A2 (en) 2014-02-11
EP3301180A1 (en) 2018-04-04
EP2121941B1 (en) 2015-10-28
US20120199148A1 (en) 2012-08-09
EP2537939A1 (en) 2012-12-26
CN101611146A (en) 2009-12-23
AP2009004905A0 (en) 2009-06-30
JP2010512751A (en) 2010-04-30
US20070199097A1 (en) 2007-08-23
WO2008076802A2 (en) 2008-06-26
JP5441708B2 (en) 2014-03-12
EP2333081A3 (en) 2011-09-07
US20100218270A1 (en) 2010-08-26
EP2121941A2 (en) 2009-11-25
EP2537939B1 (en) 2017-08-16
HK1156352A1 (en) 2012-06-08
AR064392A1 (en) 2009-04-01
TW200845887A (en) 2008-12-01
EP2333081A2 (en) 2011-06-15
CN103993023B (en) 2018-02-27
CN101611146B (en) 2014-07-02

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Legal Events

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PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

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Effective date: 20090710

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Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC MT NL PL PT RO SE SI SK TR

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