EP2066691A1

EP2066691A1 - Use of natural, recombinant and synthetic resilins in cosmetics

Info

Publication number: EP2066691A1
Application number: EP07822317A
Authority: EP
Inventors: Claus Bollschweiler; Burghard Liebmann; Marcus Fehr; Daniel HÜMMERICH; Arne Ptock; Evelyn Raunft
Original assignee: BASF SE
Current assignee: BASF SE
Priority date: 2006-11-08
Filing date: 2007-11-07
Publication date: 2009-06-10
Also published as: WO2008055931A1; US20100015070A1; CA2664033A1; JP2010509279A

Abstract

Use of resilin proteins in cosmetics, characterised in that the resilin proteins contain between one and one hundred repetition units with the consensus sequence SXXYGXP, where S = serine, X = any amino acid, Y = tyrosine, G = glycine and P = proline.

Description

Use of natural, recombinant and synthetic resilins in cosmetics

The present invention relates to the use of natural, recombinant and synthetic Resilinproteinen in cosmetics, in particular in the skin cosmetics.

State of the art

WO 2004/104042 describes the preparation of a natural proresilin fragment and the further processing by cross-linking into a bioelastomer and its use.

WO 2004/104043 describes protein sequences based on repetitive sequences of natural Resilinproteine, as well as the further processing by cross-linking to bioelastomers and their use.

Description of the invention

The present invention relates to novel cosmetic or dermatological compositions which contain as active ingredient at least one natural, recombinant or synthetic resiline of insects or an analogue of such a protein.

More particularly, the present invention relates to compositions which are presented as products for hair treatment, skin care products, make-up products, or sun protection products based on a resilin protein of insects.

The use of proteins is well known; in cosmetics, they are frequently used, in particular silk proteins of the caterpillar Bombyx mori, this use being described in detail in Kaplan et al .: Silks; Biomaterials. Novel Materials from Biological Sources1-53 Stockton press; New York (1991). However, the silk proteins do not have good elastic properties when they are in the form of filaments or films, and therefore can not satisfactorily serve as film formers. The properties of Resilin are described in Gosline et al .; Philosophical Transactions of the Royal Society of London - Series B: Biological Sciences. 357 (1418): 121-32, (2002). They are known for their good elastic properties and the high proportion of elastically stored energy (resilience).

It has now been found that resilin proteins, in addition to their known properties, can impart unexpected properties to cosmetic or dermatological compositions and, in particular, can improve the hydrating or softening effect. Incidentally, the Resilines behave like good film formers and, above all, have a low surface density.

The present invention therefore relates, as a new technical product, to a cosmetic or dermatological composition containing an effective amount of at least one natural recombinant or synthetic resin or an analog of such a protein.

According to one embodiment of the cosmetic compositions according to the invention, the resilins are preferably natural resilin which is obtained from insects.

According to a specific embodiment of the cosmetic compositions according to the invention, the resilins are preferably recombinant resilins of insects. For this purpose, a natural DNA sequence coding for Resilin or Resilin fragments is isolated from insects, heterologously expressed in an expression organism and the recombinant Resilinprotein isolated therefrom. The nucleotide sequence of the isolated DNA can be further modified by molecular biological methods.

According to a preferred embodiment of the cosmetic compositions according to the invention, the resilins are preferably synthetic resilins. Gene sequences coding for protein sequences based on repeating units of natural resilin proteins are synthesized by chemical and molecular biological methods. The synthetic genes are heterologously expressed in an expression organism and the synthetic resilin protein is isolated therefrom. The recombinant and synthetic resilins have the advantage that they have a peptide sequence whose physicochemical properties can be adjusted depending on the desired cosmetic or dermatological applications.

The compositions according to the invention may contain at least one natural or recombinant or synthetic Resilinprotein in an effective amount.

Suitable for the preparation of cosmetic or dermatological compositions are Resilinproteine containing a plurality of repeating units, preferably 1-100, more preferably 10-40, and advantageously 15-33 repeating units with the consensus sequence SXXYGXP, where S is a serine, X is any Amino acid, Y is a tyrosine, G is a glycine and P is a proline. Preferably, X is selected from the group of charged and polar amino acids.

In a preferred embodiment of the invention, the repeating unit contains the consensus sequence:

Xi X2 X3 _X4 X5 S Xβ YG X7P Xe X9 X10 X11 wherein

Xi any or no amino acid

X2 any or no amino acid

X3 any or no amino acid

X ₄ is proline, alanine, threonine or serine S serine

X5 is a charged or polar amino acid

Xβ is a charged or polar amino acid

Y is a tyrosine

G is a glycine X7, a proline, alanine, threonine or serine

P a proline

Xe is a glycine, alanine, threonine or serine

Xg is a glycine a polar amino acid or no amino acid

X10 a glycine is a polar amino acid or no amino acid X11 is any or no amino acid. In one embodiment, Xi is, if present, a G, Y, A or N, preferably a G. In one embodiment, X2, if present, is a basic amino acid, G, L, Q, preferably G.

In one embodiment, X3, if present, is R, K, T or P; X ₄ is preferably a P;

X5 is preferably a D, T or S; Xβ is preferably an S, Q or T; X7 is preferably an A; Xs is preferably a G; Xg, if present, is preferably a G, Q or S, most preferably a G; X10, if present, is preferably a G, S or N, most preferably a G; X11 if present is preferably a G, Q, P, S or N.

In one embodiment, at least one of the amino acids Xe, Xg and X10 is a G, preferably

As used herein, the term consensus sequence refers to an amino acid (or nucleotide) sequence that uniquely identifies amino acids found at a particular position in terms of residue and position, while other amino acids are not specified, but instead of wildcard X instead of the amino acid commonly used one letter codes for amino acids. The one-letter code for amino acids used herein is known to those skilled in the art.

The repeating units of the resilin proteins may be the same or different.

The repeating units may be linked by linkers which preferably contain 1 to 30 amino acids, more preferably 1 to 20 amino acids. The amino acid sequence of a linker can be derived from other proteins, preferably structural proteins, or have no natural role model or be completely absent.

The distance of an amino acid within a repeat unit to the equivalency amino acid within the adjacent repeat unit may be 8 to 50 amino acids, preferably 8 to 34, and more preferably 10 to 25 and most preferably 10 to 16 amino acids. Any number of repeating units, preferably from 1 to 100, more preferably from 10 to 65, and most preferably from 15 to 35, may be added together.

In one embodiment, the protein contains the repeat unit GAPGGGNGGRPSDTY or GAPGGGNGGRPSSSY.

In a further embodiment, the sequence of the protein or parts thereof corresponds to SEQ ID NO 1 or parts of this sequence. Amino acid 1-15 of SEQ ID NO: 1 represents a T7 tag fused to the actual resilin protein. The resilin proteins according to the invention can be produced with or without such tags. It is also possible to replace the T7 tag with other similarly effective tags that can facilitate the identification and isolation of the resilin proteins.

In a further embodiment, the sequence of the protein or parts thereof corresponds to SEQ ID NO 3 or parts of this sequence.

In a further embodiment, the sequence of the protein or parts thereof corresponds to the peptide sequence or parts thereof of the resilin protein from other insects such as e.g. Anopheles gambiae, Apis mellifera, Ctenocephalides felis, Haematobia irritans exigua. The peptide sequences of these proteins are published in WO04 / 104042, which is incorporated herein by reference.

In another embodiment, the resilin protein is part of a hybrid protein. The hybrid protein consists of one or more resilin or derived sequences or fragments of these sequences, as well as one or more further protein sequences or fragments of these sequences. These further proteins may preferably be structural proteins, such as e.g. Silk protein, spider silk protein, keratin, elastin, collagen, gluten, abductin, mussel byssus protein.

The production of recombinant and synthetic Resilinproteinen takes place by expression of natural or synthetically produced gene sequences. Methods for the isolation and modification of natural gene sequences are known to the person skilled in the art.

One way of producing synthetic gene sequences is in Huemmerich et al .; Biochemistry. 43 (42): 13604-12, (2004). The expression systems for proteins are well known and have been described in Sambrook et al.: Molecular cloning: A Laboratory Manual; 3rd Ed. CoId Spring Harbor Laboratory Press; CoId Spring Harbor (2001).

Nonlimiting examples of prokaryotic expression organisms are Escherichia coli, Bacillus subtilis, Bacillus megaterium, Corynebacterium glutamicum, etc. Nonlimiting examples of eukaryotic expression organisms are yeasts such as Saccharomyces cerevisiae, Pichia pastoris and others, filamentous fungi such as Aspergillus niger, Aspergillus oryzae, Aspergillus nidulans, Trichoderma reesei, Acremonium chrysoum, etc., mammalian cells, such as Heia cells, COS cells, CHO cells, among others, insect cells, such as Sf9 cells, MEL cells, among others, plants or plant cells, such as Solanum tuberosum, Nicotiana, and others ,

In the compositions of the invention, the term "effective amount" of at least one natural, recombinant or synthetic resilin protein of insects or an analogous compound corresponds to an amount of from about 0.001 to 30% by weight, but preferably from 0.01 to 15% by weight. %, based on the total weight of the composition, wherein the proportion may vary depending on the type of cosmetic or dermatological composition.

In addition to the polypeptide sequences shown above, particularly functional equivalents, functional derivatives and salts of this sequence are also preferred.

According to the invention, "functional equivalents" are in particular also understood as meaning mutants which, in at least one sequence position of the abovementioned amino acid sequences, have a different amino acid than the one specifically mentioned but nevertheless possess one of the abovementioned properties. "Functional equivalents" thus include those represented by multiple amino acid additions, substitutions, deletions and / or inversions of available mutants, said changes may occur in any sequence position, as long as they lead to a mutant with the property profile according to the invention. Functional equivalence is especially given when the reactivity patterns between mutant and unchanged polypeptide are qualitatively consistent.

"Functional equivalents" in the above sense are also "precursors" of the described polypeptides as well as "functional derivatives" and "salts" of the polypeptides. "Precursors" are natural or synthetic precursors of the polypeptides with or without the desired biological activity.

Examples of suitable amino acid substitutions are shown in the following table:

Original rest Examples of substitution

AIa Ser

Arg Lys

Asn GIn; His

Asp Glu

Cys Ser

GIn Asn

Giu Asp

GIy Pro

His Asn; Gin

Ne Leu; Val

Leu Ne; Val

Lys Arg; GIn; Glu

Met Leu; ne

Phe Met; Leu; Tyr

Ser Thr

Thr Ser

Trp Tyr

Tyr Trp; Phe

VaI Ne; Leu

Salts are understood as meaning both salts of carboxyl groups and acid addition salts of amino groups of the protein molecules of the invention. [Salze Salze] Salts of carboxyl groups can be prepared in a manner known per se and include inorganic salts such as, for example, sodium, calcium, ammonium, egg salts and salts with organic bases, such as, for example, amines, such as triethanolamine, arginine, lysine, piperidine, etc. Acid addition salts, for example salts with mineral acids, such as hydrochloric acid or sulfuric acid, and salts with organic acids, such as acetic acid and Oxalic acid are also the subject of the invention.

"Functional derivatives" of polypeptides of the invention may be attached to functional amino acid side groups or to their N- or C-terminal end by known means Techniques are also produced. Such derivatives include, for example, aliphatic esters of carboxylic acid groups, amides of carboxylic acid groups, obtainable by reaction with ammonia or with a primary or secondary amine; N-acyl derivatives of free amino groups prepared by reaction with acyl groups; or O-acyl derivatives of free hydroxy groups prepared by reaction with acyl groups.

Further objects of the invention are molecules which consist of couplings of a Resilinprotein and a effector molecule. All proteins already mentioned above are suitable as protein.

In the following, the term "effector molecule" refers to molecules which have a certain, predictable effect. These may be either proteinaceous molecules such as enzymes or non-proteinogenic molecules such as dyes, sunscreens, vitamins, provitamins, antioxidants and fatty acids, conditioners or metal ion-containing compounds.

Among the proteinaceous effector molecules, enzymes and proteins with specific binding properties are preferred.

Among the enzymes, the following are preferred as effector molecules: oxidases, peroxides, proteases, glucanases, mutanase, tyrosinases, laccases, metal binding enzymes, lactoperoxidase, lysozyme, amyloglycosidase, glucose oxidase, superoxide dismutase, photolyase, T4 endonuclease, catalase, thioredoxin, thioredoxin Reductase.

In the case of the proteinaceous but non-enzymatically active effector molecules, the following are preferred as effector molecules: hydrophobins, collagen, carotenoid-binding proteins, heavy-metal-binding proteins, odorant-binding proteins, cellulose-binding proteins, starch-binding proteins, keratin-binding proteins.

Also suitable as proteinaceous effector molecules are hydrolysates of proteins from plant and animal sources, for example hydrolyzates of proteins of marine origin.

Among the non-proteinaceous effector molecules, carotenoids are preferred. According to the invention, carotenoids are to be understood as meaning the following compounds and their esterified or glycosylated derivatives: .beta.-carotene, lycopene, lutein, astaxanthin, zeaxanthin, cryptoxanthin, citranaxanthin, canthaxanthin, bixin, .beta.-apo-4-carotenal, .beta.-apo 8 -carotinal, β-apo-8-carotenoic acid ester, neurospores, echinenone, adonirubin, violaxxin, torules, torularhodin, singly or as a mixture. Preferably used Rotinoids are beta-carotene, lycopene, lutein, astaxanthin, zeaxanthin, citranaxanthin and canthaxanthin.

Further preferred effector molecules are UV light protection filters. By this are meant organic substances capable of absorbing ultraviolet rays and absorbing the absorbed energy in the form of longer wavelength radiation, e.g. Heat, give it up again. The organic substances may be oil-soluble or water-soluble.

As oil-soluble UV-B filters, e.g. the following substances are used:

3-benzylidene camphor and its derivatives, e.g. 3- (4-methylbenzylidene) camphor;

4-aminobenzoic acid derivatives, preferably 2-ethylhexyl 4- (dimethylamino) benzoate, 2-octyl 4- (dimethylamino) benzoate and 4- (dimethylamino) benzoic acid ester;

Esters of cinnamic acid, preferably 2-ethylhexyl 4-methoxycinnamate, 4-propyl methoxy cinnamate, isoamyl 4-methoxycinnamate, 4-isoacetyl methoxycinnamate, 2-cyano-3-phenylcinnamic acid 2-ethylhexyl ester (octocrylene);

Esters of salicylic acid, preferably 2-ethylhexyl salicylate, 4-isopropylbenzyl salicylate, homomenthyl salicylate;

Derivatives of benzophenone, preferably 2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxy-4'-methylbenzophenone, 2,2'-dihydroxy-4-methoxybenzophenone;

Esters of benzalmalonic acid, preferably di-2-ethylhexyl 4-methoxybenzmalonate;

Triazine derivatives, such as 2,4,6-trianilino- (p-carbo-2'-ethyl-1 ^'-hexyloxy) -1, 3,5-triazine (octyl tyltriazone) and Dioctyl Butamido Triazone (Uvasorb HEB ^®):

Propane-1,3-diones, e.g. 1- (4-tert-butylphenyl) -3- (4'-methoxyphenyl) propane-1,3-dione.

Suitable water-soluble substances are:

2-phenylbenzimidazole-5-sulfonic acid and its alkali, alkaline earth, ammonium, alkylammonium, alkanolammonium and glucammonium salts; Sulfonic acid derivatives of benzophenones, preferably 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid and its salts;

Sulfonic acid derivatives of the 3-benzylidene camphor, e.g. 4- (2-Oxo-3-bornylidenemethyl) benzenesulfonic acid and 2-methyl-5- (2-oxo-3-bomylidene) -sulfonic acid and its salts.

Especially preferred is the use of esters of cinnamic acid, preferably 4-methoxycinnamic acid 2-ethylhexyl ester, 4-methoxycinnamic acid isopentyl ester, 2-cyano-3-phenylcinnamic acid 2-ethylhexyl ester (octocrylene).

Furthermore, the use of derivatives of benzophenone, in particular 2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxy-4 ^" -methylbenzophenone, 2,2'-dihydroxy-4-methoxybenzophenone and the use of propane-1, 3-diones, such as 1- (4-tert-butylphenyl) -3- (4-methethoxyphenyl) propane-1,3-dione.

Typical UV-A filters are:

Derivatives of benzoylmethane, such as 1- (4'-tert-butylphenyl) -3- (4'-methoxyphenyl) propane-1,3-dione, 4-tert. Butyl 4'-methoxydibenzoylmethane or 1-phenyl-3- (4'-isopropylphenyl) -propane-1,3-dione;

Amino-hydroxy-substituted derivatives of benzophenones, e.g. N, N-diethylamino-hydroxybenzoyl-n-hexylbenzoate.

Of course, the UV-A and UV-B filters can also be used in mixtures.

Suitable UV filter substances are mentioned in the following table.

In addition to the two aforementioned groups of primary light stabilizers, it is also possible to use secondary light stabilizers of the antioxidant type which interrupt the photochemical reaction chain which is triggered when UV radiation penetrates into the skin. Typical examples of these are superoxide dismutase, catalase, tocopherols (vitamin E), coenzyme Q10, ubiquinones, quinones and ascorbic acid (vitamin C).

Another group are anti-irritants that have an anti-inflammatory effect on UV-damaged skin. Such substances are, for example, bisabolol, phytol and phytantriol.

According to the invention, effector molecules are furthermore inorganic pigments which block UV radiation. Preference is given to pigments based on metal oxides and / or other sparingly water-soluble or insoluble metal compounds selected from the group consisting of the oxides of zinc (ZnO), titanium (TiO 2), iron (eg Fe 2 O 3), zirconium (ZrO 2), silicon (SiO 2), Manganese (eg MnO), aluminum (Al2O3), Cers (eg Ce2O3), mixed oxides of the corresponding metals and mixtures of such oxides.

The inorganic pigments may be present in coated form, i. that they are superficially treated. This surface treatment can be, for example, that the pigments are provided in a manner known per se, as described in DE-A-33 14 742, with a thin hydrophobic layer.

Further preferred effector molecules are vitamins, especially vitamin A and their esters. Retinoids in the context of the present invention are vitamin A alcohol (retinol) and its derivatives such as vitamin A aldehyde (retinal), vitamin A acid (retinoic acid) and vitamin A esters (eg retinyl acetate, retinyl propionate and retinyl palmitate). The term retinoic acid encompasses both all-trans retinoic acid and 13-cis retinoic acid. The terms retinol and retinal preferably include the all-trans compounds. The preferred retinoid used for the suspensions of the invention all-trans-retinol, hereinafter referred to as retinol.

Further preferred effector molecules are vitamins, provitamins and vitamin precursors from groups A, C, E and F, in particular 3,4-didehydroretinol, .beta.-carotene (provitamin of vitamin A), ascorbic acid (vitamin C) and the palmitic acid esters, glucosides or phosphates of ascorbic acid, tocopherols, especially α-tocopherol and its esters, eg the acetate, nicotinate, phosphate and succinate; vitamin F, which is understood as meaning essential fatty acids, especially linoleic acid, linolenic acid and arachidonic acid.

The vitamins, provitamins or vitamin precursors of the vitamin B group or derivatives thereof which are preferably to be used according to the invention and the derivatives of 2-furanone include, inter alia:

Vitamin B ₁ , trivial name thiamine, chemical name 3 - [(4'-amino-2'-methyl-5'-pyrimidinyl) methyl] -5- (2-hydroxyethyl) -4-methylthiazolium chloride.

Vitamin B ₂ , trivial name riboflavin, chemical name 7,8-dimethyl-10- (1-D-ribityl) -benzo [g] pteridine-2,4 (3H, 10H) -dione. In free form riboflavin z. As in whey, other riboflavin derivatives can be isolated from bacteria and yeasts. A stereoisomer of riboflavin which is also suitable according to the invention is lyxoflavin which can be isolated from fishmeal or liver and carries a D-arabityl residue instead of D-ribityl.

Vitamin B ₃ . Under this name, the compounds nicotinic acid and nicotinamide (niacinamide) are often performed. According to the invention, the nicotinic acid amide is preferred.

Vitamin B ₅ (pantothenic acid and panthenol). Panthenol is preferably used. Derivatives of panthenol which can be used according to the invention are, in particular, the esters and ethers of panthenol and also cationically derivatized panthenols. In a further preferred embodiment of the invention, it is also possible to use derivatives of 2-furanone in addition to pantothenic acid or panthenol. Particularly preferred derivatives are the commercially available substances dihydro-3-hydroxy-4,4-dimethyl-2 (3H) -furanone with the trivial name pantolactone (Merck), 4 hydroxymethyl-γ- butyrolactone (Merck), 3,3-dimethyl-2-hydroxy-γ-butyrolactone (Aldrich) and 2,5-dihydro-5-methoxy-2-furanone (Merck), expressly including all stereoisomers.

Advantageously, these effector molecule compounds impart moisturizing and skin-soothing properties to the resilin proteins according to the invention.

Vitamin B ₆ , which is understood here not a single substance, but the known under the common names pyridoxine, pyridoxamine and pyridoxal derivatives of 5-hydroxymethyl-2-methylpyridin-3-ols.

Vitamin B ₇ (biotin), also known as vitamin H or "skin vitamin". Biotin is (3aS, 4S, 6aR) -2-oxohexahydrothienol [3,4-d] imidazole-4-valeric acid.

Panthenol, pantolactone, nicotinamide and biotin are very particularly preferred according to the invention.

According to the invention, suitable derivatives (salts, esters, sugars, nucleotides, nucleosides, peptides and lipids) of said compounds can be used as effector molecules. As lipophilic, oil-soluble antioxidants from this group, tocopherol and its derivatives, gallic acid esters, flavonoids and carotenoids, and butylhydroxytoluene / anisole are preferred. As water-soluble antioxidants are amino acids, eg. As tyrosine and cysteine and their derivatives and tanning agents, especially those of plant origin are preferred.

More preferred are so-called peroxide decomposed, i. Compounds which are able to decompose peroxides, particularly preferably lipid peroxides. By this are meant organic substances, such as e.g. Pyridine-2-thiol-3-carboxylic acid, 2-methoxy-pyrimidinol-carboxylic acids, 2-methoxy-pyridinecarboxylic acids, 2-dimethylaminopyrimidinolcarboxylic acids, 2-dimethylaminopyridinecarboxylic acids.

Triterpenes, in particular triterpenic acids such as ursolic acid, rosmarinic acid, betulinic acid, boswellic acid and bryonic acid.

Another preferred effector molecule is lipoic acid and suitable derivatives (salts, esters, sugars, nucleotides, nucleosides, peptides and lipids).

Further preferred effector molecules are fatty acids, in particular saturated fatty acids which carry an alkyl branching, particularly preferably branched eicosanoic acids, such as 18-methyl-eicosanoic acid. Further preferred effector molecules are dyes, for example food dyes, semipermanent dyes, reactive or oxidation dyes. In the case of the oxidation dyes, it is preferable to link a component as effector molecule with the Resilinproteinen and then to couple at the site of action, ie after application to skin, oxidatively with the second dye component. In the case of oxidation dyes, it is also preferable to carry out the coupling of the color components before the linkage with the resilin proteins.

The reactive dyes can furthermore preferably be linked to the resilin proteins as a component as the effector molecule and then applied to the skin. Furthermore, those dyes which are linked as effector molecule with the Resilinproteinen be applied by application to skin in decorative cosmetics.

Suitable dyestuffs are all customary hair dyestuffs for the molecules according to the invention. Suitable dyes are the expert from manuals of cosmetics such as Schrader, bases and formulations of cosmetics, Hüthig Verlag, Heidelberg, 1989, ISBN 3-7785-1491-1 known.

Particularly advantageous dyes are those mentioned in the following list. The Color Index Numbers (CIN) are taken from the Rowe Color Index, 3rd Edition, Society of Dyers and Colourists, Bradford, England, 1971.

Also suitable are food dyes as dyes.

The effector molecules are linked to the resilin protein. The connection between effector molecule and protein and may be both a covalent bond and based on ionic or van der Waals interactions or hydrophobic interactions or hydrogen bonds or adsorption.

The covalent linking of the effector molecule can take place via the side chains of the polypeptide sequence of the resilin protein, in particular via amino functions or hydroxyl functions or carboxylate functions or thiol functions. Preferred is a linkage via the amino functions of one or more lysine residues, via the carboxylate functions of one or more glutamate or aspartate residues, via the thiol function of one or more cysteine residues or via the N-terminal or C-terminal function of the resilin protein. In addition to the amino acid functions found in the resilin protein sequence, amino acids with suitable functions (e.g., cysteines, lysines, aspartates, glutamates) can also be added to the sequence or inserted into the sequence, or amino acids of the resilin protein can be substituted by such amino acid functions.

The linkage of the effector molecules with the Resilinprotein can be done directly, ie as a covalent linkage of two existing chemical functions. The chemical functions of the resilin proteins described above are linked to reactive groups contained in the effector molecules. Examples of such reactive groups are sulfhydryl-reactive groups, for example maleimides, pyridyl disulfides, α-haloacetyls, vinyl sulfones, sulfatoalkyl sulfones (preferably sulfatoethyl sulfones), amine-reactive groups (for example succinimidyl esters, carbodiimde, hydroxymethyl phosphine, imidoester, PFP). Ester, aldehyde, isothiocyanate, etc.), carboxy-reactive groups (eg, amines, etc.), hydroxyl-reactive groups (eg, isocyanates, etc.), unselective groups (eg, arylazides, etc.), and photoactivatable groups (eg, perfluorophenyl azide, etc.). If the functions of the resilin protein and the effector to be coupled have too little reactivity for a direct coupling, then these functions can be carried out by a person skilled in the art activated by conventional methods (eg activation of carboxyl functions with carbodiimides).

The linkage can also be via a so-called linker, i. An at least bifunctional molecule is formed which binds with one or more functions with the effector molecule and is linked to the resilin protein with one or more other functions. The use of such tailored linker allows the exact adaptation of the linkage to the desired effector molecule. In addition, it is thereby possible to link several effector molecules with a resident protein in a defined manner.

The linker used depends on the functionality to be coupled. Suitable are e.g. Molecules that couple to a Resilinproteine means

Sulfhydryl-reactive groups, e.g. Maleimides, pydridyl disulfides, α-haloacetyls, vinylsulfones, sulfatoalkyl sulfones (preferably sulfatoethyl sulfones), amine-reactive groups (eg succinimidyl esters, carbodiimde, hydroxymethyl phosphine, imido esters, PFP esters, aldehyde, isothiocyanate etc.), carboxy-reactive groups ( eg amines, etc.), hydroxyl-reactive groups (eg isocyanates etc.), unselective groups (eg arylazides etc.) and photoactivatable groups (eg perfluorophenyl azide etc.) and to effector molecules by means of - sulfhydryl-reactive groups (eg maleimides, pydridyl disulfides , α-haloacetyls, vinylsulfones, sulfatoalkylsulfones (preferably sulfatoethylsulfones), amine-reactive groups (eg succinimidyl ester, carbodiimde, hydroxymethyl-phosphine, imidoester, PFP-ester, aldehyde, isothiocyanate, etc.) sugars or oxidized sugar-reactive groups (eg hydrazides, etc .) - Carboxy-reactive groups (eg amines etc.)

Hydroxyl-reactive groups (e.g., isocyanates, etc.), thymine-reactive groups (e.g., psoralen, etc.), nonselective groups (e.g., aryl azides, etc.), photoactivatable groups (e.g., perfluorophenyl azide, etc.), metal-complexing groups (e.g., EDTA, hexahis, ferritin).

Antibodies and fragments (e.g., single-chain antibodies, F (ab) fragments of antibodies, catalytic antibodies).

The chemical functions of a linker may be linked by spacer elements. Spacerelemete can be composed of, for example, alkyl chains, ethylene glycol and polyethylene glycols.

Particularly preferred are linker and / or spacer elements which have a potential cleavage site for a protease, lipase, esterase, phosphatase, hydrolase, ie are enzymatically cleavable. Examples of enzymatically cleavable linkers which can be used in the molecules according to the invention are mentioned, for example, in WO 98/01406, to the entire contents of which reference is hereby expressly made. Particularly preferred are linkers and spacers which are thermally dissociable, photocleavable. Corresponding chemical structures are known to the person skilled in the art and are integrated between the molecule parts effector molecule and resilin protein.

If the effector molecule consists of a polypeptide sequence, the linkage of effector and resilin protein may take place as a so-called fusion protein, i. a continuous polypeptide sequence composed of effector and resine protein subsequences is used.

It is also possible to incorporate so-called spacer elements between effector and resilin protein, for example polypeptide sequences which have a potential cleavage site for a protease, lipase, esterase, phosphatase, hydrolase or ONgo and polypeptide sequences which permit easy purification of the fusion protein, For example, so-called His-tags, ie Oligohistidinreste.

The covalently or non-covalently coupled to Resilinproteine effector molecules may be active in their bonded form. Alternatively, the effector molecules coupled to resilin proteins can also be released from the resilin proteins.

The release of covalently coupled effector molecules from the resiliproteins can be achieved by cleavage of specifically incorporated cleavable spacers or coupling linkers, e.g. thermally cleavable, photocleavable or enzymatically cleavable, but also by proteolytic degradation (e.g., by proteases).

For the use according to the invention in cosmetics, the Resilinproteine be formulated with conventional other active ingredients and excipients found in cosmetics.

The resilin proteins according to the invention are preferably used for skin cosmetics.

Suitable auxiliaries and additives for the production of hair cosmetic, nail cosmetic or skin cosmetic preparations are familiar to the expert and can from manuals of cosmetics, such as Schrader, bases and formulations of cosmetics, Hüthig Verlag, Heidelberg, 1989, ISBN 3-7785-1491-1 , are taken.

The cosmetic agents according to the invention may be skin-cosmetic, nail-cosmetic, hair-cosmetic, dermatological, hygienic or pharmaceutical agents. The agents according to the invention are preferably in the form of a gel, foam, spray, ointment, cream, emulsion, suspension, lotion, milk or paste. If desired, liposomes or microspheres can also be used.

The cosmetically or pharmaceutically active agents according to the invention may additionally contain cosmetically and / or dermatologically active agents as well as excipients.

The cosmetic compositions according to the invention preferably comprise at least one resilin protein and at least one different constituent selected from cosmetically active ingredients, emulsifiers, surfactants, preservatives, perfume oils, thickeners, hair polymers, hair and skin conditioners, graft polymers, water-soluble or dispersible silicone-containing polymers, Sunscreens, bleaches, gelling agents, conditioners, colorants, tinting agents, tanning agents, dyes, pigments, bodying agents, moisturizers, restoats, collagen, protein hydrolysates, lipids, antioxidants, defoamers, antistatic agents, emollients and plasticizers. The Resilinproteine can also be contained in encapsulated form in the cosmetic preparations.

Advantageously, the antioxidants are selected from the group consisting of amino acids (eg glycine, histidine, tyrosine, tryptophan) and their derivatives, imidazolones (eg urocaninic acid) and their derivatives, peptides such as D, L-carnosine, D-carnosine, L Carnosine and its derivatives (eg anserine), carotenoids, carotenes (eg .beta.-carotene, lycopene) and their derivatives, chlorogenic acid and its derivatives, lipoic acid and derivatives thereof (eg dihydrolipoic acid), aurothioglucose, propylthiouracil and other thiols (eg thiorodoxin , Glutathione, cysteine, cystine, cystamine and their glycosyl, N-acetyl, methyl, ethyl, propyl, amyl, butyl, and lauryl, palmitoyl, oleyl, γ-linoleyl, chlorine, lesteryl and glyceryl esters) and their salts, dilauryl thiodipropionate, distearyl thiodipropionate, thiodipropionic acid and derivatives thereof (esters, ethers, peptides, lipids, nucleotides, nucleosides and salts) and sulfoximine compounds (eg buthionine sulfoximines, homocysteinesulfoximines, buthionine sulfones, pentaamides). , Hexa-, heptathionine-sulfoximine) in very low compatible dosages (eg pmol to μmol / kg), furthermore (metal) chelators (eg α-hydroxyfatty acids, palmitic acid, phytic acid, lactoferrin), α-hydroxy acids (eg citric acid, Lactic acid, malic acid), humic acid, bile acids, bile extracts, bilirubin, biliverdin, EDTA and their derivatives, unsaturated fatty acids and their derivatives (eg γ-linolenic acid, linoleic acid, oleic acid), folic acid and its derivatives, ubiquinone and ubiquinol and derivatives thereof, Vitamin C and its derivatives (eg, sodium ascorbate, ascorbyl palmitate, Mg ascorbyl phosphate, ascorbyl acetate), tocopherol and derivatives (eg, vitamin E acetate, tocotrienol), vitamin A and derivatives (vitamin A palmitate), and benzylic resin, rutinic acid, and benzylic acid their derivatives, α-glycosylrutin, ferulic acid, furfurylidene glucitol, carnosine, butylhydroxytoluene, butylated hydroxyanisole, nordihydroguaiacetic acid, nordihydroguiaretic acid, triisobutyric acid, hydroxybutyrophenone, uric acid and its derivatives, mannose and its derivatives, zinc and its derivatives (eg ZnO, ZnSO ₄ ), selenium and its derivatives (eg selenium methionine), stilbenes and their derivatives (eg stilbene oxide, trans-stilbene oxide).

Also advantageous are so-called peroxide decomposers, i. Compounds which are able to decompose peroxides, particularly preferably lipid peroxides. By this are meant organic substances, such as e.g. Pyridine-2-thiol-3-carboxylic acid, 2-methoxy-pyrimidinol-carboxylic acids, 2-methoxy-pyridinecarboxylic acids, 2-dimethylaminopyrimidinolcarboxylic acids, 2-dimethylaminopyridinecarboxylic acids.

Typical thickeners in such formulations are crosslinked polyacrylic acids and their derivatives, polysaccharides and their derivatives, such as xanthan gum, agar-agar, alginates or tyloses, cellulose derivatives, e.g. Carboxymethylcellulose or hydroxycarboxymethylcellulose, fatty alcohols, monoglycerides and fatty acids, polyvinyl alcohol and polyvinylpyrrolidone. Nonionic thickeners are preferably used.

Suitable cosmetically and / or dermatologically active agents are e.g. coloring active substances, skin and hair pigmenting agents, tinting agents, suntanning agents, bleaching agents, keratin-hardening substances, antimicrobial agents, light-filtering active ingredients, repellent active ingredients, hyperemic substances, keratolytic and keratoplasmic substances, antidandruff active ingredients, antiphlogistics, keratinizing substances, antioxidant or as antioxidants Radical scavengers active ingredients, skin moisturizing or moisturizing substances, moisturizing agents, anti-erythemic or anti-allergic active ingredients, branched fatty acids such as 18-Methyleicosansäure, and mixtures thereof.

Artificial skin tanning agents which are suitable for tanning the skin without natural or artificial irradiation with UV rays are e.g. Dihydroxyacetone, alloxan and walnut shark extract. Suitable keratin-hardening substances are, as a rule, active ingredients as are also used in antiperspirants, such as, for example, antiperspirants. Potassium aluminum sulfate, aluminum hydroxychloride, aluminum lactate, etc.

Antimicrobial agents are used to destroy microorganisms or to inhibit their growth and thus serve both as a preservative and as a deodorizing substance, which reduces the formation or intensity of body odor. These include, for example, customary preservatives known to the person skilled in the art, such as p-hydroxybenzoic acid ester, imidazolidinyl urea, formaldehyde, sorbic acid, benzoic acid, salicylic acid, etc. Such substances having a deodorizing action are, for example, zinc ricinoleate, triclosan, undecylenic acid alkylolamides, citric acid triethyl lester, chlorhexidine etc. Suitable preservatives which are listed below with their E number are to be used advantageously according to the invention.

Furthermore, preservatives or preservatives which are common in cosmetics according to the invention are dibromodicyanobutane (2-bromo-2-bromomethylglutarodinitrile), 3-iodo-2-propynyl butylcarbamate, 2-bromo-2-nitro-propane-1,3-diol, imidazolidinyl urea, 5-chloro - 2-methyl-4-isothiazolin-3-one, 2-chloroacetamide, benzalkonium chloride, benzyl alcohol suitable, Formaldehydabspalter.

Furthermore, phenylhydroxyalkyl ethers, in particular the compounds known as phenoxyethanol, are suitable as preservatives because of their bactericidal and fungicidal effects on a number of microorganisms.

Other germ-inhibiting agents are also suitable in the invention

Preparations to be incorporated. Advantageous substances are, for example, 2,4,4'-trichloro-2'-hydroxydiphenyl ether (Irgasan), 1, 6-di- (4-chlorphenylbiguanido) - hexane (chlorhexidine), 3,4,4'-trichlorocarbanilide, quaternary ammonium compounds , Clove oil, mint oil, thyme oil, triethyl citrate, farnesol (3,7,1-trimethyl-2,6,10-dodecatrien-1-ol) and in the patent publications DE-37 40 186, DE-39 38 140, DE -42 04 321, DE-42 29 707, DE-43 09 372, DE-44 11 664, DE-195 41 967, DE-195 43 695, DE-195 43 696, DE-195 47 160, DE-196 02 108, DE-196 02 110, DE 196 02 11 1, DE-196 31 003, DE-196 31 004 and DE-196 34 019 and the patents DE-42 29 737, DE-42 37 081, DE-43 24 219, DE-44 29 467, DE -44 23 410 and DE-195 16 705 described active ingredients or drug combinations. Also, sodium bicarbonate is advantageous to use.

Suitable light filter active substances are substances which absorb UV rays in the UV-B and / or UV-A range. Suitable UV filters are e.g. 2,4,6-triaryl-1, 3,5-triazines in which the aryl groups can each bear at least one substituent, which is preferably selected from hydroxy, alkoxy, especially methoxy, alkoxycarbonyl, especially methoxycarbonyl and ethoxycarbonyl and mixtures thereof , Also suitable are p-aminobenzoic acid esters, cinnamic acid esters, benzophenones, camphor derivatives and UV-radiation-stopping pigments, such as titanium dioxide, talc and zinc oxide.

Suitable UV filter substances are any UV-A and UV-B filter substances. For example:

The cosmetic and dermatological preparations according to the invention may advantageously also contain UV-blocking inorganic pigments based on metal oxides and / or other sparingly soluble or insoluble metal compounds selected from the group of the oxides of zinc (ZnO), titanium (TiO ₂ ), iron ( eg Fe ₂ O ₃ ), zirconium (ZrO ₂ ), silicon (SiO ₂ ), manganese (eg MnO), aluminum (Al ₂ O ₃ ), cerium (eg Ce ₂ O ₃ ), mixed oxides of the corresponding metals and mixtures thereof Contain oxides.

The inorganic pigments can be present in coated form, ie they are treated on the surface. This surface treatment can be, for example, that the pigments are provided in a manner known per se, as described in DE-A-33 14 742, with a thin hydrophobic layer. Suitable repellent agents are compounds capable of preventing or repelling certain animals, particularly insects, from humans. These include, for example, 2-ethyl-1,3-hexanediol, N, N-diethyl-m-toluamide, etc. Suitable hyperemic substances which stimulate the perfusion of the skin are, for example, essential oils, such as mountain pine extract, lavender extract, rosemary extract, juniper berry extract, Horse chestnut extract, birch leaf extract, hay flower extract, ethyl acetate, camphor, menthol, peppermint oil, rosemary extract, eucalyptus oil, etc. Suitable keratolytic and keratoplastic substances are, for example, salicylic acid, calcium thioglycolate, thioglycolic acid and its salts, sulfur, etc. Suitable antidandruff active substances are, for example, sulfur Sulfur polyethylene glycol sorbitan monooleate, sulfur ricinol polyethoxylate, zinc pyrithione, aluminum pyrithione, etc. Suitable antiphlogistic agents which counteract skin irritation include allantoin, bisabolol, dragosantol, chamomile extract, panthenol, etc.

The cosmetic agents according to the invention may contain as cosmetic and / or pharmaceutical active ingredient (as well as optionally as excipient) at least one cosmetically or pharmaceutically acceptable polymer. These include, in general, cationic, amphoteric and neutral polymers.

Suitable polymers are e.g. cationic polymers called polyquaternium according to INCI, e.g. Copolymers of vinylpyrrolidone / N-vinylimidazolium salts (Luviquat FC, Luviquat HM, Luviquat MS, Luviquat & commat, Care), copolymers of N-vinylpyrrolidone / dimethylaminoethyl methacrylate, quaternized with diethyl sulfate (Luviquat PQ 11), copolymers of N-vinylcaprolactam / N-vinylpyrrolidone / N

Vinylimidazolium salts (Luviquat E Hold), cationic cellulose derivatives (polyquaternium-4 and -10), acrylamidocopolymers (Polyquaternium-7) and chitosan.

Suitable cationic (quaternized) polymers are also merquat (dimethyl diallyl ammonium chloride based polymer), gafquat (quaternary polymers formed by reaction of polyvinyl pyrrolidone with quaternary ammonium compounds), polymer JR (hydroxyethyl cellulose with cationic groups), and plant based cationic polymers, e.g. Guarpolymers, such as the Jaguar brands of Rhodia.

Further suitable polymers are also neutral polymers, such as polyvinylpyrrolidones, copolymers of N-vinylpyrrolidone and vinyl acetate and / or vinyl propionate, polysiloxanes, polyvinylcaprolactam and other copolymers with N-vinylpyrrolidone, polyethylenimines and their salts, polyvinylamines and their salts, cellulose derivatives, Polyasparaginic acid salts and derivatives. These include, for example, Luviflex 0 Swing (partially hydrolysed copolymer of polyvinyl acetate and polyethylene glycol, BASF). Suitable polymers are also nonionic, water-soluble or water-dispersible polymers or oligomers, such as polyvinylcaprolactam, for example Luviskol 0 Plus (BASF), or polyvinylpyrrolidone and their copolymers, in particular with vinyl esters, such as vinyl acetate, for example Luviskol 0 VA 37 (BASF), polyamides , For example, based on itaconic acid and aliphatic diamines, as described for example in DE-A-43 33 238.

Suitable polymers are also amphoteric or zwitterionic polymers, such as those available under the names Amphomer (National Starch) octylacrylamide / methyl methacrylate / tert-butylaminoethyl methacrylate hydroxypropyl methacrylate copolymers and zwitterionic polymers, as described for example in German patent applications DE39 29 973, DE 21 50 557, DE 28 17 369 and DE 3708 451 are disclosed. Acrylamidopropyl trimethylammonium chloride / acrylic acid or. Methacrylic acid copolymers and their alkali metal and ammonium salts are preferred zwitterionic polymers. Further suitable zwitterionic polymers are methacroylethylbetaine / methacrylate copolymers, which are commercially available under the name Amersette (AMERCHOL), and copolymers of hydroxyethyl methacrylate, methyl methacrylate, N, N-dimethylaminoethyl methacrylate and acrylic acid (Jordapon (D)).

Suitable polymers are also nonionic, siloxane-containing, water-soluble or -dispersible polymers, e.g. Polyether siloxanes, such as Tegopren 0 (Goldschmidt) or Besi & commat (Wacker).

The formulation base of cosmetic agents according to the invention preferably contains cosmetically and / or pharmaceutically acceptable excipients. Pharmaceutically acceptable excipients which are known to be useful in the pharmaceutical, food technology and related fields, in particular those listed in relevant pharmacopoeias (e.g., DAB Ph. Eur. BP NF), as well as other excipients whose properties do not preclude physiological application.

Suitable auxiliaries may be: lubricants, wetting agents, emulsifying and suspending agents, preserving agents, antioxidants, anti-irritants, chelating agents, emulsion stabilizers, film formers, gelling agents, odor masking agents, resins, hydrocolloids, solvents, solubilizers, neutralizing agents, permeation accelerators, pigments, quaternary ammonium compounds, Rest grease and superfatting agents, ointment, cream or oil bases, silicone derivatives, stabilizers, sterilants, blowing agents, drying agents, opacifiers, thickeners, waxes, softeners, white oil. A related embodiment is based on expert knowledge, as shown for example in Fiedler, HP Lexicon of excipients for pharmacy, cosmetics and related fields, 4th ed., Aulendorf: ECV Editio-Kantor-Verlag, 1996. To prepare the dermatological agents of the invention, the active ingredients may be mixed or diluted with a suitable excipient (excipient). Excipients may be solid, semi-solid or liquid materials which may serve as a vehicle, carrier or medium for the active ingredient. If desired, the admixing of further auxiliaries takes place in the manner known to the person skilled in the art. Furthermore, the polymers and dispersions are suitable as auxiliaries in pharmacy, preferably as or in coating agent (s) or binder (s) for solid dosage forms. They can also be used in creams and as tablet coatings and tablet binders.

According to a preferred embodiment, the agents according to the invention are a skin cleanser.

Preferred skin cleansing agents are soaps of liquid to gelatinous consistency, such as transparent soaps, luxury soaps, deep soaps, cream soaps, baby soaps, skin soaps, abrasive soaps and syndets, pasty soaps, soft soaps and washing pastes, peeling soaps, moisturizing wipes, liquid washing, showering and bathing preparations such as Washing lotions, shower baths and gels, bubble baths, oil baths and scrub preparations, shaving foams, lotions and creams.

According to a further preferred embodiment, the agents according to the invention are cosmetic agents for the care and protection of the skin and hair, nail care preparations or preparations for decorative cosmetics.

Suitable skin cosmetic agents are e.g. Face lotions, face masks, deodorizers and other cosmetic lotions. Means for use in decorative cosmetics include, for example, masking pens, theatrical paints, mascara and eye shadows, lipsticks, kohl pencils, eyeliners, rouges, powders, and eyebrow pencils.

In addition, the dermatological compositions according to the invention can be used in nose strips for pore cleansing, in anti-acne agents, repellents, shaving agents, after- and pre-shave care products, after-sun care products, hair removal preparations, hair dyeing preparations, personal care products, foot care products and in baby care.

The skin care compositions according to the invention are in particular W / O or O / W skin creams, day and night creams, eye creams, face creams, anti-wrinkle creams, sunscreen creams, moisturizing creams, bleaching creams, self-tanning creams, vitamin creams, skin lotions, skin lotions and moisturizing lotions.

Skin cosmetic and dermatological compositions based on the previously described resine proteins show advantageous effects. The Resilinproteine can contribute, inter alia, to the moisturization and conditioning of the skin and to improve the skin feel. The Resilinproteine can also be used as a thickener in the formulations Act. By adding the Resilinproteine invention can be achieved in certain formulations, a significant improvement in skin compatibility.

Skin cosmetic and dermatological compositions preferably contain at least one Resilinprotein in a proportion of about 0.001 to 30 wt .-%, preferably 0.01 to 20 wt .-%, most preferably 0.1 to 12 wt .-%, based on the total weight of the agent.

Particularly light stabilizers based on the Resilinproteine have the property to increase the residence time of the UV-absorbing ingredients in comparison to common adjuvants such as polyvinylpyrrolidone.

Depending on the field of application, the compositions according to the invention may be in a form suitable for skin care, such as e.g. as a cream, foam, gel, pen, mousse, milk, spray (pump spray or propellant spray) or lotion can be applied.

The skin-cosmetic preparations may contain, in addition to the Resilinproteinen and suitable carriers, other common in skin cosmetics agents and excipients, as described above. These preferably include emulsifiers, preservatives, perfume oils, cosmetic active ingredients such as phytantriol, vitamins A, E and C, retinol, bisabolol, panthenol, light stabilizers, bleaching agents, colorants, tinting agents, suntanning agents, collagen, enzymes, protein hydrolysates, stabilizers, pH - Regulators, dyes, salts, thickeners, gelling agents, bodying agents, silicones, humectants, moisturizers and other common additives.

Preferred oil and fat components of the skin cosmetic and dermatological agents are the aforementioned mineral and synthetic oils, e.g. Paraffins, silicone oils and aliphatic hydrocarbons having more than 8 carbon atoms, animal and vegetable oils, e.g. Sunflower oil, coconut oil, avocado oil, olive oil, lanolin, or waxes, fatty acids, fatty acid esters, e.g. Triglycerides of C6-C30 fatty acids, wax esters, e.g. Jojoba oil, fatty alcohols, vaseline, hydrogenated lanolin and acetylated lanolin, and mixtures thereof.

You can mix the Resilinproteine invention also with conventional polymers, if special properties are to be set.

To adjust certain properties, such as, for example, improving the feeling of touch, the spreading behavior, the water resistance and / or the binding of active substances and excipients, such as pigments, the skin-cosmetic and dermatological preparations may additionally contain conditioning substances based on silicone compounds. Suitable silicone compounds are, for example, polyalkylsiloxanes, polyarylsiloxanes, polyarylalkylsiloxanes, polyethersiloxanes or silicone resins.

The preparation of the cosmetic or dermatological preparations is carried out by customary methods known to the person skilled in the art.

The cosmetic and dermatological agents are preferably in the form of emulsions, in particular as water-in-oil (W / O) or oil-in-water (O / W) emulsions.

But it is also possible to choose other types of formulations, for example, gels, oils, oil gels, multiple emulsions, for example in the form of W / O / W or O / W / O emulsions, anhydrous ointments or ointment bases, etc. Also emulsifier-free formulations such as hydrodispersions, hydrogels or a Pickering emulsion are advantageous embodiments.

Emulsions are prepared by known methods. In addition to at least one resilin protein, the emulsions generally contain customary constituents, such as fatty alcohols, fatty acid esters and, in particular, fatty acid triglycerides, fatty acids, lanolin and derivatives thereof, natural or synthetic oils or waxes and emulsifiers in the presence of water. The selection of the emulsion type-specific additives and the preparation of suitable emulsions is described for example in Schrader, bases and formulations of cosmetics, Hüthig book Verlag, Heidelberg, 2nd edition, 1989, third part, which is hereby incorporated by reference.

A suitable emulsion as W / O emulsion, e.g. for a skin cream, etc., generally contains an aqueous phase which is emulsified by means of a suitable emulsifier system in an oil or fat phase. To provide the aqueous phase, a polyelectrolyte complex can be used.

Preferred fat components which may be included in the fat phase of the emulsions are: hydrocarbon oils such as paraffin oil, purcellin oil, perhydrosqualene and solutions of microcrystalline waxes in these oils; animal or vegetable oils, such as sweet almond oil, avocado oil, calophylum, lanolin and derivatives thereof, castor oil, Se Samoel, olive oil, jojoba oil, karite oil, hoplostethus oil, mineral oils, their distillation start under atmospheric pressure at about 250 ⁰ C and a distillation end point at 410 ⁰ C, such as vaseline oil, esters of saturated or unsaturated fatty acids, such as alkyl, including i-propyl, butyl or cetyl myristate, hexadecyl stearate, ethyl or i-propyl palmitate, octanoic or Decansäuretriglyceride and cetyl ricinoleate.

The fat phase may also contain other oil-soluble silicone oils such as dimethylpolysiloxane, methylphenylpolysiloxane and the silicone glycol copolymer, fatty acids and fatty alcohols. In addition to the Resilinproteinen also waxes can be used, such as carnauba wax, candililla wax, beeswax, microcrystalline wax, ozokerite wax and Ca, Mg and Al oleates, myristates, linoleates and stearates.

Furthermore, an emulsion of the invention may be present as O / W emulsion. Such an emulsion usually contains an oil phase, emulsifiers that stabilize the oil phase in the water phase, and an aqueous phase that is usually thickened. Suitable emulsifiers are preferably O / W emulsifiers, such as polyglycerol esters, sorbitan esters or partially esterified glycerides into consideration.

According to a further preferred embodiment, the agents according to the invention are a shower gel, a shampoo formulation or a bathing preparation.

Such formulations contain at least one resilin protein and usually anionic surfactants as base surfactants and amphoteric and / or nonionic surfactants as cosurfactants. Other suitable active ingredients and / or auxiliaries are generally selected from lipids, perfume oils, dyes, organic acids, preservatives and antioxidants, as well as thickeners / gelling agents, skin conditioners and moisturizers.

These formulations preferably contain from 2 to 50% by weight, preferably from 5 to 40% by weight, particularly preferably from 8 to 30% by weight, of surfactants, based on the total weight of the formulation.

In the washing, shower and bath preparations all anionic, neutral, amphoteric or cationic surfactants commonly used in personal care products can be used.

Suitable anionic surfactants are, for example, alkyl sulfates, alkyl ether sulfates, alkyl sulfonates, alkylaryl sulfonates, alkyl succinates, alkyl sulfosuccinates, N-alkoyl sarcosylates, acyl taurates, acyl isothionates, alkyl phosphates, alkyl ether phosphates, alkyl ether carboxylates, alpha-olefin sulfonates, especially the alkali and alkaline earth metal salts, e.g. Sodium, potassium, magnesium, calcium, as well as ammonium and triethanolamine salts. The alkyl ether sulfates, alkyl ether phosphates and alkyl ether carboxylates can have between 1 to 10 ethylene oxide or propylene oxide units, preferably 1 to 3 ethylene oxide units in the molecule.

These include, for example, sodium lauryl sulfate, ammonium taurate sulfate, sodium lauryl ether sulfate, ammonium lauryl ether sulfate, sodium lauryl sarcosinate, sodium oleyl succinate, ammonium umlaurylsulfosuccinate, sodium dodecylbenzenesulfonate, triethanolamine dodecylbenzenesulfonate.

Suitable amphoteric surfactants are e.g. Alkylbetaines, alkylamidopropylbetaines, alkylsulfobetaines, alkylglycinates, alkylcarboxyglycinates, alkylamphoacetates or -propionates, alkylamphodiacetates or -dipropionates.

For example, cocodimethylsulfopropyl betaine, lauryl betaine, cocamidopropyl betaine or sodium cocamphopropionate can be used.

Suitable nonionic surfactants are, for example, the reaction products of aliphatic alcohols or alkylphenols having 6 to 20 C atoms in the alkyl chain, which may be linear or branched, with ethylene oxide and / or propylene oxide. The amount of alkylene oxide is about 6 to 60 moles per mole of alcohol. Also suitable are alkylamine oxides, mono- or dialkylalkanolamides, fatty acid esters of polyethylene glycols, ethoxylated fatty acid amides, alkylpolyglycosides or sorbitan ether esters.

In addition, the washing, showering and bathing preparations may contain conventional cationic surfactants, e.g. quaternary ammonium compounds, for example cetyltrimethylammonium chloride.

Furthermore, the shower gel / shampoo formulations may contain thickeners, e.g. Common salt, PEG-55, propylene glycol oleate, PEG-120 methyl glucose dioleate and others, as well as preservatives, other active ingredients and auxiliaries and water.

According to a further preferred embodiment, the agents according to the invention are a hair treatment agent.

Hair treatment agents according to the invention preferably contain at least one Resilinprotein in an amount in the range of about 0.01 to 30 wt .-%, preferably 0.5 to 20 wt .-%, based on the total weight of the composition.

Preferably, the hair treatment compositions of the present invention are in the form of a mousse, hair mousse, hair gel, shampoo, hair spray, hair mousse, top fluid, perming, hair dyeing and bleaching or hot oil treatments. Depending on the field of application, the hair cosmetic preparations can be applied as (aerosol) spray, (aerosol) foam, gel, gel spray, cream, lotion or wax. Hairsprays include both aerosol sprays and pump sprays without propellant gas. Hair foams include both aerosol foams and pump foams without propellant gas. Hair sprays and hair foams preferably comprise predominantly or exclusively water-soluble or water-dispersible components. Are those in the hair sprays and hair foams of the invention water-dispersible compounds used, they can be used in the form of aqueous microdispersions with particle diameters of usually 1 to 350 nm, preferably 1 to 250 nm, are used. The solids contents of these preparations are usually in a range of about 0.5 to 20 wt .-%. As a rule, these microdispersions do not require emulsifiers or surfactants for their stabilization.

In a preferred embodiment, the hair cosmetic formulations according to the invention comprise a) from 0.01 to 30% by weight of at least one resilin protein, b) from 20 to 99.95% by weight of water and / or alcohol, c) from 0 to 50% by weight at least a propellant, d) 0 to 5 wt .-% of at least one emulsifier, e) 0 to 3 wt .-% of at least one thickener, and up to 25 wt .-% further constituents.

By alcohol are meant all alcohols customary in cosmetics, e.g. Ethanol, isopropanol, n-propanol.

Other ingredients are understood to include the additives customary in cosmetics, for example propellants, defoamers, surface-active compounds, i. Surfactants, emulsifiers, foaming agents and solubilizers. The surface-active compounds used can be anionic, cationic, amphoteric or neutral. Other common ingredients may also be e.g. Preservatives, perfume oils, opacifiers, active ingredients, UV filters, care ingredients such as panthenol, collagen, vitamins, protein hydrolysates, alpha and beta hydroxycarboxylic acids, stabilizers, pH regulators, dyes, viscosity regulators, gel formers, salts, humectants, moisturizers, complexing agents and other common additives.

Furthermore, this includes all known in cosmetics styling and conditioner polymers that can be used in combination with the Resilinproteinen invention, if very special properties are to be set.

Suitable conventional hair cosmetic polymers include, for example, the abovementioned cationic, anionic, neutral, nonionic and amphoteric polymers, to which reference is hereby made.

To adjust certain properties, the preparations may additionally contain conditioning substances based on silicone compounds. Suitable silicone compounds are, for example, polyalkylsiloxanes, polyarylsiloxanes, polyarylalkylsiloxanes, polyethersiloxanes, silicone resins or dimethicone copolyols (CTFA) and amino-functional silicone compounds such as amodimethicones (CTFA).

The polymers according to the invention are particularly suitable as setting agents in hairstyling preparations, in particular hair sprays (aerosol sprays and pump sprays without propellant gas) and hair foams (aerosol foams and pump foams without propellant gas).

In a preferred embodiment, spray formulations contain a) 0.01 to 30 wt .-% of at least one Resilinproteins, b) 20 to 99.9 wt .-% of water and / or alcohol, c) 0 to 70 wt .-% at least a blowing agent, d) 0 to 20 wt .-% further ingredients.

Blowing agents are the blowing agents commonly used for hairsprays or aerosol foams. Preference is given to mixtures of propane / butane, pentane, dimethyl ether, 1,1-difluoroethane (HFC-152a), carbon dioxide, nitrogen or compressed air.

A preferred formulation for aerosol hair foams according to the invention comprises a) from 0.01 to 30% by weight of at least one resilin protein, b) from 55 to 99.8% by weight of water and / or alcohol, c) from 5 to 20% by weight of a blowing agent , d) from 0.1 to 5% by weight of an emulsifier, e) from 0 to 10% by weight of further constituents.

As emulsifiers all emulsifiers commonly used in hair foams can be used. Suitable emulsifiers may be nonionic, cationic or anionic or amphoteric.

Examples of nonionic emulsifiers (INCI nomenclature) are Laurethe, e.g. Laureth-4; Cetethe, e.g. Cetheth-1, polyethylene glycol cetyl ether, ceteareth, e.g. Cethetereth-25, polyglycol fatty acid glycerides, hydroxylated lecithin, lactyl esters of fatty acids, alkylpolyglycosides.

Examples of cationic emulsifiers are cetyldimethyl-2-hydroxyethylammonium dihydrogenphosphate, cetyltrimonium chloride, cetyltrimmonium bromide, cocotrimonium methylsulfate, quaternium-1 to x (INCI).

Anionic emulsifiers may, for example, be selected from the group of alkyl sulfates, alkyl ether sulfates, alkyl sulfonates, alkylaryl sulfonates, alkyl succinates, alkyl sulfosuccinates, N-alkoyl sarcosinates, acyl taurates, acyl isethionates, alkyl phosphates, alkyl ether phosphates, alkyl ether carboxylates, alpha olefin sulfonates, especially the alkali and alkaline earth metal salts, e.g. Sodium, potassium, magnesium, calcium, as well

Ammonium and triethanolamine salts. The alkyl ether sulfates, alkyl ether phosphates and alkyl ether carboxylates can have between 1 to 10 ethylene oxide or propylene oxide units, preferably 1 to 3 ethylene oxide units in the molecule.

A preparation suitable for styling gels according to the invention can be composed, for example, as follows: a) 0.01 to 30% by weight of at least one resilin protein, b) from 80 to 99.85% by weight of water and / or alcohol, c) from 0 to 3% by weight, preferably from 0.05 to 2% by weight, of a gelling agent, d) from 0 to 20% by weight other ingredients. The use of gelling agents may be advantageous to adjust for specific rheological or other performance properties of the gels. As gel formers it is possible to use all gel formers customary in cosmetics. These include slightly crosslinked polyacrylic acid, for example carbomer (INCI), cellulose derivatives, for example hydroxypropylcellulose, hydroxyethylcellulose, cationically modified celluloses, polysaccharides, for example xanthan gum, caprylic / capric triglyceride, sodium acrylate copolymers, polyquaternium-32 (and) paraffin liquidum (INCI), Sodium acrylate copolymers (and) Paraffinum Liquidum (and) PPG-1 trideceth-6, acrylamidopropyltrimonium chloride / acrylamide copolymers, steareth-10-allyl ethers, acrylate copolymers, Polyquaternium-37 (and) Paraffinum Liquidum (and) PPG-1 Trideceth-6, polyquaternium 37 (and) propylene glycol dicaprate dicaprylate (and) PPG-1 trideceth-6, polyquaternium-7, polyquaternium-44.

The Resilinproteine invention can be used in cosmetic preparations as conditioning agents.

A preparation containing the resilin proteins according to the invention can preferably be used in shampoo formulations as a setting and / or conditioning agent. Preferred shampoo formulations comprise a) from 0.01 to 30% by weight of at least one resilin protein, b) from 25 to 94.95% by weight of water, c) from 5 to 50% by weight of surfactants, c) from 0 to 5% by weight. % of a further conditioning agent, d) 0 to 10 wt .-% further cosmetic ingredients.

In the shampoo formulations all anionic, neutral, amphoteric or cationic surfactants commonly used in shampoos can be used.

Suitable anionic surfactants are, for example, alkyl sulfates, alkyl ether sulfates, alkyl sulfonates, alkylaryl sulfonates, alkyl succinates, alkyl sulfosuccinates, N-

Alkoyl sarcosinates, acyl taurates, acyl isothionates, alkyl phosphates, alkyl ether phosphates, alkyl ether carboxylates, alpha olefin sulfonates, especially the alkali and alkaline earth metal salts, e.g. Sodium, potassium, magnesium, calcium, and ammonium and triethanolamine salts. The alkyl ether sulfates, alkyl ether phosphates and alkyl ether carboxylates can have between 1 to 10 ethylene oxide or propylene oxide units, preferably 1 to 3 ethylene oxide units in the molecule.

Suitable examples are sodium lauryl sulfate, ammonium lauryl sulfate, sodium lauryl sulfate, ammonium lauryl ether sulfate, sodium lauroyl sarcosinate, sodium oleyl succinate, ammonium lauryl sulfosuccinate, sodium dodecylbenzenesulfonate, triethanolamine dodecylbenzenesulfonate. Suitable amphoteric surfactants are, for example, alkylbetaines, alkylamidopropylbetaines, alkylsulfobetaines, alkylglycinates, alkylcarboxyglycinates, alkylamphoacetates or -propionates, alkylamphodiacetates or -dipropionates.

Suitable nonionic surfactants are, for example, the reaction products of aliphatic alcohols or alkylphenols having 6 to 20 C atoms in the alkyl chain, which may be linear or branched, with ethylene oxide and / or propylene oxide. The amount of alkylene oxide is about 6 to 60 moles per mole of alcohol. Also suitable are alkylamine oxides, mono- or dialkylalkanolamides, fatty acid esters of polyethylene glycols, alkylpolyglycosides or sorbitan ether esters.

In addition, the shampoo formulations may contain conventional cationic surfactants, e.g. quaternary ammonium compounds, for example cetyltrimethylammonium chloride.

In the shampoo formulations, customary conditioning agents in combination with the resilin proteins can be used to achieve certain effects.

These include, for example, the aforementioned cationic polymers with the name Polyquaternium according to INCI, in particular copolymers of vinylpyrrolidone / N-vinylimidazolium salts (Luviquat FC, Luviquat & commat, HM, Luviquat MS, Luviquat Care), copolymers of N-vinylpyrrolidone / dimethylaminoethyl methacrylate, quaternized with diethyl sulfate ( Luviquat D PQ 1 1), copolymers of N-vinylcaprolactam / N-vinylpyrrolidone / N-vinylimidazolium salts (Luviquat D Hold), cationic cellulose derivatives (Polyquaternium-4 and -10), acrylamide copolymers (Polyquaternium-7). Furthermore, protein hydrolysates can be used, as well as conditioning substances based on silicone compounds, for example polyalkylsiloxanes, polyarylsiloxanes, polyarylalkylsiloxanes, polyethersiloxanes or silicone resins. Other suitable silicone compounds are dimethicone copolyols (CTFA) and amino-functional silicone compounds such as amodimethicones (CTFA). Furthermore, cationic guar derivatives such as guar hydroxypropyltrimonium chloride (INCI) can be used.

Another object of the invention is the preparation and use of basic Resilinen. Basic Resilines are characterized in that the ratio of the basic amino acid residues (K, R) to the acidic amino acid residues (D, E) contained in the repeating units which form the Resilinprotein at least 1, 5, preferably at least 2. Particularly advantageous basic resilins are those in which the ratio of basic to acidic amino acid residues at min. at least 40% of the repeat units is 1: 1 and at least 40% of the repeat units is 1: 0.

Particularly preferred is the preparation and use of basic Resilinprotei- nen, the repeat unit GAPGGGNGGRPSDTY or

GAPGGGNGGRPSSSY or functional equivalents of this repeating unit included. Particularly preferred is the preparation and use of the synthetic Resilinproteins Ui6 (SEQ ID NO 1) and the nucleotide sequence coding for it (SEQ ID NO 2).

Basic Resilinproteine are particularly suitable for use in cosmetics, for the formulation of substances, for the paper, leather and textile finishing, for coating surfaces and for use in human and animal nutrition.

Experimental part

example 1

Production of the synthetic resilin protein Ui6

Gene Synthesis and Expression A synthetic gene encoding the resilin protein Ui6 was prepared by multimerizing a synthetic oligonucleotide U: 5'-ggtgcgccgggcggtggcaacggtggccgtccgtctgacacctacggtgcgccgggtggcggacacggtggccgtccttctctctcttac-3 'as described in Huemmerich et al .; Biochemistry. 43 (42): 13604-12, (2004) to a 16mer multimerized and cloned into the expression vector pET21 a (Novagen). The expression took place in the strain E. coli BLR [DE3] (Novagen). The expressed protein U16 with the sequence SEQ ID NO: 1 contains N-terminally a T7 tag (amino acid numbers 1-15) and subsequently from amino acid no. 16 the actual resilin protein. Cultivation and protein synthesis were carried out at 37 ° C., pO 2> 20% and pH = 6.8 in the fed-batch method.

Medium 8 liters of water 230 g Yeast extract 150 g Bacto-tryptone 30 g glycerol (99%) 20 g potassium dihydrogen phosphate (KH2PO4), 50 g of ammonium sulfate ((NH ₄₎ ₂ SO ₄₎ 10.5 g of magnesium sulfate heptahydrate (MgSO ₄ ^* 7 H2O)

Add 1, 0 g calcium chloride dihydrate (CaCl ₂ ^* 2 H ₂ O) 100 ml trace acid solution with water to 9.7 liters adjust pH to 6,8 with 25% NaOH 3 ml Tego KS 91 1 (Antifoam, Goldschmidt products) 1 g of ampicillin

Trace salt solution:

5 liters of water

200.00 g of citric acid monohydrate

55.00 g ZnSO ₄ ^* 7 H ₂ O

42.50 g (NH ₄₎ ₂ Fe (SO ₄₎ ₂ ^* 6 H ₂ O

15.00 g MnSO ₄ ^* H ₂ O

4.00 g _CuSO4 ^* 5 _H2O

1, 25 g CoSO ₄ ^* 7 H ₂ O

Feeding solution 800 g of water

30 g of citric acid monohydrate 60 g of sodium sulfate (Na ₂ SO ₄₎ 4.5 g (NH ₄₎ 2 Fe (SO ₄₎ 2 ^* 6 H ₂ O 3400 g of glycerol 99.5%

After using up the glycerol contained in the basic medium, a constant feed was started at a rate of 100 ml / h.

Protein synthesis was induced by addition of 1 mM isopropyl-β-D-thiogalactopyranoside after the bacterial culture reached an OD6oo = 40 optical density. At this time, the temperature of the culture was lowered to 30 ⁰ C. 10 h after induction, the cells were harvested.

The purification of the protein was carried out according to the following protocol: Resuspension of the cell pellet in 5 ml per gram of wet mass 20 mM MOPS

(3- (N-morpholino) propanesulfonic acid) pH 7.0

Disruption of the cells in the high-pressure homogenizer

Centrifugation 30 min, 5000xg

Dissolve the pellet by adding 1.3 g of guanidinium hydrochloride (GdmHCl) per gram of pellet (final concentration 6 M GuaHCl)

Dialysis against 20 mM citric acid pH 2.65

Centrifugation 45 min, 10000xg

Adjusting the pH of the supernatant to 7.0 by adding 25%

NaOH. - Centrifugation 45 min, 10000xg

Precipitate the protein from the supernatant by adding one volume

4 M ammonium sulfate

Centrifugation 45 min, 10000xg

The protein thus purified may be used as ammonium sulfate precipitate at -20 ⁰ C STORED.

For the use of the protein, the ammonium sulfate precipitate is in

GuaHCI, dialyzed against citric acid and neutralized.

The protein content in solution was determined photometrically (E (28o, img / mi) = O, 93)

The purity of the protein was checked by SDS-PAGE and by fluorescence spectroscopy (Fig. 1 + 2).

From a 10 l fermentation 700 mg of clean Ui6 protein with a purity of> 95% were obtained. In the following dermocosmetische preparations according to the invention are described, containing the Resilinproteine prepared according to Example 1

Example 3: Use Resilinprotein in a emulsion for day care - Type O / W

WS 1%:

% Ingredient (INCI)

A 1, 7 Ceteareth-6, Stearyl Alcohol

0.7 Ceteareth-25

2.0 diethylamino hydroxybenzoyl hexyl benzoate

2.0 PEG-14 Dimethicone

3.6 Cetearyl Alcohol

6.0 ethylhexyl methoxycinnamate

2.0 dibutyl adipate

B 5.0 glycerin

0.2% disodium EDTA

1, 0 panthenol

1, 0 Resilinprotein q.s. preservative

67.8 Aqua the.

C 4.0 Caprylic / Capric Triglyceride, Sodium Acrylate Copolymer

D 0.2 Sodium Ascorbyl Phosphate

1, 0 tocopheryl acetate

0.2 bisabolol

1, 0 Caprylic / Capric Triglyceride, Sodium Ascorbate, Tocopherol, Retinol

Eq. S. Sodium hydroxides

WS 5%:

% Ingredient (INCI)

1, 7 Ceteareth-6, Stearyl Alcohol

0.7 Ceteareth-25

2.0 diethylamino hydroxybenzoyl hexyl benzoate

2.0 PEG-14 Dimethicone

3.6 Cetearyl Alcohol

6.0 ethylhexyl methoxycinnamate

2.0 dibutyl adipate B 5.0 glycerol

0.2% disodium EDTA

1, 0 panthenol

5.0 Resilin protein q.s. preservative

63.8 Aqua the.

4.0 Caprylic / Capric Triglyceride, Sodium Acrylate Copolymer D 0.2 Sodium Ascorbyl Phosphate

1, 0 tocopheryl acetate

0.2 bisabolol

1, 0 Caprylic / Capric Triglyceride, Sodium Ascorbate, Tocopherol, Retinol

E q.s. Sodium hydroxides

Preparation: Heat phases A and B separately from each other to about 80 ^° C. Stir phase B into phase A and homogenize. Stir phase C into combined phases A and B and homogenize again. Cool to about 40 ^° C. with stirring, add phase D, adjust the pH to about 6.5 with phase E, homogenize and cool to room temperature while stirring.

Note: The formulation is produced without inert gas. The filling must be in oxygen-impermeable packaging, e.g. Aluminum tubes take place.

Example 4: Use Resilinprotein in a Protective Day Cream - Type O / W

WS 1%:

% Ingredient (INCI)

A 1, 7 Ceteareth-6, Stearyl Alcohol

0.7 Ceteareth-25

2.0 diethylamino hydroxybenzoyl hexyl benzoate

2.0 PEG-14 Dimethicone

3.6 Cetearyl Alcohol

6.0 ethylhexyl methoxycinnamate

2.0 dibutyl adipate

B 5.0 glycerin

0.2% disodium EDTA

1, 0 panthenol

1, 0 Resilinprotein q.s. preservative

68.6 Aqua the.

C 4.0 Caprylic / Capric Triglyceride, Sodium Acrylate Copolymer

D 1, 0 Sodium ascorbyl phosphates

1, 0 tocopheryl acetate

0.2 bisabolol

E q.s. Sodium hydroxides

WS 5%:

% Ingredient (INCI)

A 1, 7 Ceteareth-6, Stearyl Alcohol

0.7 Ceteareth-25

2.0 diethylamino hydroxybenzoyl hexyl benzoate

2.0 PEG-14 Dimethicone

3.6 Cetearyl Alcohol

6.0 ethylhexyl methoxycinnamate

2.0 dibutyl adipate B 5.0 glycerin

0.2% disodium EDTA

1, 0 panthenol

5.0 Resilin protein q.s. preservative

64.6 Aqua the.

C 4.0 Caprylic / Capric Triglyceride, Sodium Acrylate Copolymer

D 1, 0 Sodium ascorbyl phosphates

1, 0 tocopheryl acetate

0.2 bisabolol

E q.s. Sodium hydroxides

Preparation: Heat phases A and B separately from each other to about 80 ^° C. Stir phase B into phase A and homogenize. Prepare phase C in combined phases A and B and homogenize. Cool to about 40 ^° C. while stirring. Add phase D, adjust the pH to about 6.5 with phase E and homogenize. Cool to room temperature while stirring.

Example 5: Use of Resilin Protein in a Facial Cleansing Lotion - Type O / W WS 1%:

% Ingredient (INCI)

A 10.0 Cetearyl ethylhexanoate

10.0 Caprylic / Capric Triglycerides

1, 5 cyclopentasiloxanes, cyclohexasilosans 2.0 PEG-40 Hydrogenated Castor OiI

B3.5 Caprylic / Capric Triglycerides, Sodium Acrylates Copolymer

C 1, 0 tocopheryl acetate

0.2 bisabolol q.s. Preservatives q.s. perfume oil

D 3.0 polyquaternium-44

0.5 cocotrimonium methosulfates

0.5 ceteareth-25

2.0 Panthenol, Propylene Glycol 4.0 Propylene Glycol

0.1% disodium EDTA

1, 0 Resilinprotein

60.7 Aqua.

WS 5%:

% Ingredient (INCI)

A 10.0 Cetearyl ethylhexanoate

10.0 Caprylic / Capric Triglycerides

1, 5 cyclopentasiloxanes, cyclohexasilosans 2.0 PEG-40 Hydrogenated Castor OiI

B3.5 Caprylic / Capric Triglycerides, Sodium Acrylates Copolymer

C 1, 0 tocopheryl acetate 0.2 bisabolol qs preservative qs perfume oil

D 3.0 polyquaternium-44

0.5 cocotrimonium methosulfates

0.5 ceteareth-25

2.0 Panthenol, Propylene Glycol

4.0 Propylene Glycol

0.1% disodium EDTA

5.0 Resilinprotein

56.7 Aqua the.

Preparation: Release phase A. Stir phase B into phase A, incorporate phase C into combined phases A and B. Dissolve phase D, stir into the combined phases A, B and C and homogenize. Stir for 15 minutes.

Example 6: Using Resilin Protein in a Daily Care Body Spray

WS 1%:

% Ingredient (INCI)

A 3.0 ethylhexyl methoxycinnamate

2.0 diethylamino hydroxybenzoyl hexyl benzoate

1, 0 Polyquaternium-44

3.0 Propylene Glycol

2.0 Panthenol, Propylene Glycol

1, 0 cyclopentasiloxanes, cyclohexasiloxanes

10.0 octyldodecanol

0.5 PVP

10.0 Caprylic / Capric Triglycerides

3.0 C12-15 alkyl benzoates

3.0 glycerin

1, 0 tocopheryl acetate

0.3 bisabolol

1, 0 Resilinprotein

59.2 Alcohol

WS 5%:

% Ingredient (INCI)

A 3.0 ethylhexyl methoxycinnamate

2.0 diethylamino hydroxybenzoyl hexyl benzoate

1, 0 Polyquaternium-44

3.0 Propylene Glycol

2.0 Panthenol, Propylene Glycol

1, 0 cyclopentasiloxanes, cyclohexasiloxanes

10.0 octyldodecanol

0.5 PVP

10.0 Caprylic / Capric Triglycerides

3.0 C12-15 alkyl benzoates

3.0 glycerin

1, 0 tocopheryl acetate

0.3 bisabolol

5.0 Resilinprotein

55.2 Alcohol

Preparation: Weigh in the components of phase A and dissolve clearly.

Example 7: Use of Resilin Protein in a Skin Care Gel WS 1%:

% Ingredient (INCI)

A 3.6 PEG-40 Hydrogenated Castor OiI 15.0 Alcohol

0.1 bisabolol 0.5 tocopheryl acetate qs perfume oil

B 3.0 Panthenol

0.6 carbomer

1, 0 Resilinprotein

75.4 Aqua,

C 0.8 triethanolamine

WS i 5%:

% Ingredient (INCI)

A 3.6 PEG-40 Hydrogenated Castor OiI

15.0 Alcohol

0.1 bisabolol

0.5 tocopheryl acetate q.s. perfume oil

B 3.0 Panthenol

0.6 carbomer

5.0 Resilinprotein

71, 4 Aqua,

C 0.8 triethanolamine

Preparation: Clear phase A clearly. Swell phase B and neutralize with phase C. Stir phase A into the homogenized phase B and homogenize.

Example 8: Use of Resilin Protein in an Aftershave Lotion

WS 1%:

% Ingredient (INCI)

A 10, 0 cetearyl ethylhexanoate

5, 0 tocopheryl acetate

1, 0 Bisabolol

0, 1 perfume oil

0, 3 Acrylates / C 10-30 alkyl acrylate crosspolymer

B 15, 0 Alcohol

1, 0 panthenol

3, 0 glycerol

1, 0 Resilinprotein

0, 1 triethanolamine

63, 5 Aqua the.

WS 5%:

% Ingredient (INCI)

A 10, 0 cetearyl ethylhexanoate

5, 0 tocopheryl acetate

1, 0 Bisabolol

0, 1 perfume oil

0, 3 Acrylates / C 10-30 alkyl acrylate crosspolymer

B 15 .0 Alcohol 1, 0 panthenol

3.0 glycerin

5.0 Resilinprotein

0.1 triethanolamine

59.5 Aqua the.

Preparation: Mix the components of phase A. Dissolve phase B, work in phase A and homogenize.

Example 9: Using Resilin Protein in an After Sun Lotion WS 1%:

% Ingredient (INCI)

A 0.4 Acrylates / C 10-30 Alkyl Acrylate Crosspolymer

15.0 Cetearyl ethylhexanoates

0.2 bisabolol

1, 0 tocopheryl acetate q.s. perfume oil

B 1, 0 panthenol

15.0 Alcohol

3.0 glycerin

1, 0 Resilinprotein

63.2 Aqua,

C 0.2 triethanolamine

WS 5%:

% Ingredient (INCI)

A 0.4 Acrylates / C 10-30 Alkyl Acrylate Crosspolymer

15.0 Cetearyl ethylhexanoates

0.2 bisabolol

1, 0 tocopheryl acetate q.s. perfume oil

B 1, 0 panthenol

15.0 Alcohol

3.0 glycerin

5.0 Resilinprotein

59.2 Aqua,

C 0.2 triethanolamine

Preparation: Mix the components of phase A. Stir phase B into phase A while homogenizing. Neutralize with Phase C and homogenize again.

Example 10: Use Resilinprotein- in a sunscreen lotion WS 1%:

% Ingredient (INCI) A 4,5 Ethylhexyl Methoxycinnamate

2.0 Diethylamino Hydroxybenzoyl Hexyl Benzoate 3.0 Octocrylene 2.5 di-C12-13 alkyl malates

0.5 tocopheryl acetate

4.0 polyglyceryl-3 methyl glucose distearate

B 3,5 Cetearyl isononanoate

1, 0 VP / eicosene copolymer

5.0 isohexadecane

2.5 di-C12-13 alkyl malates

3.0 Titanium dioxides, trimethoxycaprylylsilanes

C 5.0 glycerin

1, 0 Sodium Cetearyl Sulfate

0.5 xanthan gum

1, 0 Resilinprotein

59.7 Aqua the.

D 1, 0 phenoxyethanol, methylparaben, ethylparaben, butylparaben, propylparaben, isobutylparaben

0.3 bisabolol

WS 5%:

% Ingredient (INCI)

A 4,5 ethylhexyl methoxycinnamate

2.0 diethylamino hydroxybenzoyl hexyl benzoate

3.0 octocrylene

2.5 di-C12-13 alkyl malates

0.5 tocopheryl acetate

4.0 polyglyceryl-3 methyl glucose distearate

B 3,5 Cetearyl isononanoate

1, 0 VP / eicosene copolymer

5.0 isohexadecane

2.5 di-C12-13 alkyl malates

3.0 Titanium dioxides, trimethoxycaprylylsilanes

C 5.0 glycerin

1, 0 Sodium Cetearyl Sulfate

0.5 xanthan gum

5.0 Resilinprotein

55.7 Aqua.

0.3 bisabolol

Preparation: Heat the components of phases A and B separately to about 80 ^° C. Stir phase B into phase A and homogenize. Heat phase C to about 80 ^° C. and stir into the combined phases A and B while homogenizing. Cool with stirring to about 40 ° C, add phase D and homogenize again.

Example 1 1: Use Resilinprotein in a sunscreen lotion - Type O / W WS 1%: % Ingredient (INCI)

A 2.0 Ceteareth-6, Stearyl Alcohol

2.0 Ceteareth-25

3.0 tribehenin

2.0 Cetearyl Alcohol

2.0 cetearyl ethylhexanoate

5.0 ethylhexyl methoxycinnamate

1, 0 ethylhexyl triazone

1, 0 VP / eicosene copolymer

7.0 isopropyl myristate

B 5.0 Zinc oxides, triethoxycaprylylsilanes

C 0.2 xanthan gum

0.5 hydroxyethyl acrylate / sodium acryloyl dimethyl taurate copolymer,

Squalane, polysorbate 60

0.2% disodium EDTA

5.0 Propylene Glycol

0.5 panthenol

1, 0 Resilinprotein

60,9 Aqua the.

D 0.5 phenoxyethanol, methylparaben, butylparaben, ethylparaben, propylparaben, isopropylparaben

1, 0 tocopheryl acetate

0.2 bisabolol

WS 5%:

% Ingredient (INCI)

A 2.0 Ceteareth-6, Stearyl Alcohol

2.0 Ceteareth-25

3.0 tribehenin

2.0 Cetearyl Alcohol

2.0 cetearyl ethylhexanoate

5.0 ethylhexyl methoxycinnamate

1, 0 ethylhexyl triazone

1, 0 VP / eicosene copolymer

7.0 isopropyl myristate

B 5.0 Zinc oxides, triethoxycaprylylsilanes

C 0.2 xanthan gum

0.5 hydroxyethyl acrylate / sodium acryloyl dimethyl taurate copolymer,

Squalane, polysorbate 60

0.2% disodium EDTA

5.0 Propylene Glycol

0.5 panthenol

5.0 Resilinprotein

56.9 Aqua the.

D 0.5 phenoxyethanol, methylparaben, butylparaben, ethylparaben, propylparaben, isopropylparaben 1, 0 tocopheryl acetate 0.2 bisabolol

Preparation: Heat phase A to approx. 80 ^° C., stir in phase B and homogenize for 3 minutes. Also heat phase C to 80 ^° C. and stir into the combined phases A and B while homogenizing. Cool to about 40 ⁰ C, stir in phase D and homogenize again.

Example 12: Use of Resilin Protein in a Sunscreen Lotion - Type O / W

WS 1%:

% Ingredient (INCI)

A 3,5 Ceteareth-6, Stearyl Alcohol

1, 5 Ceteareth-25

7.5 ethylhexyl methoxycinnamate

2.0 diethylamino hydroxybenzoyl hexyl benzoate

2.0 cyclopentasiloxanes, cyclohexasiloxanes

0.5 Bees Wax

3.0 Cetearyl Alcohol

10.0 Caprylic / Capric Triglycerides

B 5.0 Titanium Dioxide, Silica, Methicone, Alumina

C 3.0 glycerin

0.2% disodium EDTA

0.3 xanthan gum

1, 0 decyl glucosides

2.0 Panthenol, Propylene Glycol

1, 0 Resilinprotein

56.3 Aqua the.

D 1, 0 tocopheryl acetate

0.2 bisabolol q.s. Perfume oil q.s. preservative

WS 5%:

% Ingredient (INCI)

A 3,5 Ceteareth-6, Stearyl Alcohol

1, 5 Ceteareth-25

7.5 ethylhexyl methoxycinnamate

2.0 diethylamino hydroxybenzoyl hexyl benzoate

2.0 cyclopentasiloxanes, cyclohexasiloxanes

0.5 Bees Wax

3.0 Cetearyl Alcohol

10.0 Caprylic / Capric Triglycerides

B 5.0 Titanium Dioxide, Silica, Methicone, Alumina

C 3.0 glycerin

0.2% disodium EDTA

0.3 xanthan gum 1, 0 decyl glucosides

2.0 Panthenol, Propylene Glycol

5.0 Resilinprotein

52.3 Aqua the.

D 1, 0 tocopheryl acetate

0.2 bisabolol q.s. Perfume oil q.s. preservative

Preparation: Heat phase A to approx. 80 ^° C., stir in phase B and homogenize for 3 minutes.

Also heat phase C to 80 ^° C. and stir into the combined phases A and B while homogenizing. Cool to about 40 ⁰ C, stir in phase D and homogenize again.

Example 13: Use of Resilin Protein in a Foot Balm

WS 1%:

% Ingredient (INCI)

A 2.0 Ceteareth-6, Stearyl Alcohol

2.0 Ceteareth-25

5.0 Cetearyl ethylhexanoate

4.0 Cetyl Alcohol

4.0 glyceryl stearate

5.0 mineral oil

0.2 menthol

0.5 camphor

B 69.3 Aqua the.

1, 0 Resilinprotein q.s. preservative

C 1, 0 bisabolol

1, 0 tocopheryl acetate

D 5.0 Witch Hazel Extract

WS 5%:

% Ingredient (INCI)

A 2.0 Ceteareth-6, Stearyl Alcohol

2.0 Ceteareth-25

5.0 Cetearyl ethylhexanoate

4.0 Cetyl Alcohol

4.0 glyceryl stearate

5.0 mineral oil

0.2 menthol

0.5 camphor

B 65,3 Aqua the.

5.0 Resilin protein q.s. preservative

C 1.0 bisabolol 1, 0 Tocopheryl Acetate D 5.0 Witch Hazel Extract

Preparation: The components of the phases A and B, heat up to about 80 ⁰ C separated men. Stir phase B into phase A while homogenizing. Cool with stirring to approx.

40 ⁰ C, add the phases C and D and posthumogenise shortly. Cool to room temperature while stirring.

Example 14: Use Resilinprotein in a W / O emulsion with bisabolol

WS 1%:

% Ingredient (INCI)

A 6, 0 PEG-7 Hydrogenated Castor OiI

8, 0 cetearyl ethylhexanoate

5, 0 isopropyl myristate

15, 0 Mineral OiI

0, 3 magnesium stearates

0, 3 aluminum stearates

2, 0 PEG-45 / dodecyl glycol copolymer

B 5, 0 glycerol

0, 7 magnesium sulfates

1, 0 Resilinprotein

55, 6 Aqua the.

C 0, 5 tocopheryl acetates

0, 6 bisabolol

WS 5%:

% Ingredient (INCI)

A 6, 0 PEG-7 Hydrogenated Castor OiI

8, 0 cetearyl ethylhexanoate

5, 0 isopropyl myristate

15, 0 Mineral OiI

0, 3 magnesium stearates

0, 3 aluminum stearates

2, 0 PEG-45 / dodecyl glycol copolymer

B 5, 0 glycerol

0, 7 magnesium sulfates

51, 6 Aqua the.

5, 0 Resilinprotein

C 0, 5 tocopheryl acetates

Preparation: Heat phases A and B separately from each other to approx. 85 ° C. Stir phase B into phase A and homogenize. Cool with stirring to about 40 ⁰ C, add phase C and briefly homogenize again. Cool to room temperature while stirring.

Example 15: Foam Conditioner with Fixer WS 1%

% Ingredient (INCI)

10.0 PVP / VA copolymer

0.2 hydroxyethyl cetyldimonium phosphates

0.2 Ceteareth-25

0.5 Dimethicone Copolyol q.s. perfume oil

10.0 Alcohol

1, 0 Resilinprotein

68.1 Aqua.

10.0 propane / butane

WS 5%%% Ingredient (INCI)

10.0 PVP / VA copolymer

0.2 hydroxyethyl cetyldimonium phosphates

0.2 Ceteareth-25

0.5 Dimethicone Copolyol q.s. perfume oil

10.0 Alcohol

5.0 Resilinprotein

64.1 Aqua.

10.0 propane / butane

Preparation: Weigh the components of phase A together, stir until everything is dissolved and bottled.

Example 16: Foam conditioner

WS 1%

% Ingredient (INCI)

A 1, 0 Polyquaternium-4

0.5 hydroxyethyl cetyldimonium phosphates

1, 0 Resilinprotein q.s. Perfume oil q.s. preservative

91, 5 Aqua the.

6.0 propanes / butanes

WS 5%% Ingredient (INCI) A 1, 0 Polyquaternium-4

0.5 hydroxyethyl cetyldimonium phosphates

5.0 Resilin protein q.s. Perfume oil q.s. preservative

87.5 Aqua the.

6.0 propanes / butanes

Production: Weigh the components of phase A together, stir until everything is clearly dissolved and bottled.

Example 17: Foam conditioner

WS 1%% ingredient (INCI)

A 1, 0 Polyquaternium-11

0.5 hydroxyethyl cetyldimonium phosphates

1, 0 Resilinprotein q.s. Perfume oil q.s. preservative

91, 5 Aqua the.

6.0 propanes / butanes

WS 5%

% Ingredient (INCI)

A 1, 0 Polyquaternium-11

0.5 Hydroxyethyl Cetyldimonium Phosphate 5.0 Resilin protein q.s. Perfume oil q.s. preservative

87.5 Aqua the.

6.0 propanes / butanes

Example 18: Styling foam

WS 1%

% Ingredient (INCI)

A 0.5 Laureth-4 qs perfume oil B 77.3 Aqua the.

10.0 polyquaternium-28

1, 0 Resilinprotein

0.5 dimethicone copolyol

0.2 Ceteareth-25

0.2 panthenol

0.1 PEG-25 PABA

0.2 hydroxyethylcellulose

C 10.0 HFC 152 A

WS 5%

% Ingredient (INCI)

A 0.5 Laureth-4 q.s. perfume oil

B 73,3 Aqua the.

10.0 polyquaternium-28

5.0 Resilinprotein

0.5 dimethicone copolyol

0.2 Ceteareth-25

0.2 panthenol

0.1 PEG-25 PABA

0.2 hydroxyethylcellulose

10.0 HFC 152A

Preparation: Mix the components of phase A. Add the components of phase B one by one and dissolve. Fill with phase C.

Example 19: Styling foam

WS 1%% ingredient (INCI)

A 2.0 cocotrimony methosulfate q.s. perfume oil

B 78.5 Aqua the.

6,7 acrylates copolymer

0.6 AMP

1, 0 Resilinprotein

0.5 dimethicone copolyol 0.2 ceteareth-25

0.2 panthenol

0.1 PEG-25 PABA 0.2 hydroxyethylcellulose

C 10.0 HFC 152 A

WS 5%

% Ingredient (INCI)

A 2.0 cocotrimony methosulfate q.s. perfume oil

B 74.5 Aqua the.

6,7 acrylates copolymer

0.6 AMP

5.0 Resilin Protein 0.5 Dimethicone Copolyol

0.2 Ceteareth-25

0.2 panthenol

0.1 PEG-25 PABA

0.2 hydroxyethylcellulose

C 10.0 HFC 152 A

Example 20: Styling foam

WS 1%

% Ingredient (INCI)

A 2.0 cocotrimony methosulfate q.s. perfume oil

B 7,70 Polyquaternium-44 1, 0 Resilin protein q.s. preservative

79.3 Aqua the.

C 10.0 propane / butane

WS 5%

% Ingredient (INCI)

A 2,0 cocotrimonium methosulfate qs perfume oil B 7.70 Polyquaternium-44

5.0 Resilin protein q.s. preservative

75.3 Aqua the.

C 10.0 propane / butane

Preparation: Mix the components of phase A. Clear the components of phase B, then stir phase B into phase A. Adjust the pH to 6-7, fill with phase C.

Example 21: Styling foam

WS 1%

% Ingredient (INCI)

A 2.00 cocotrimonium methosulfate q.s. perfume oil

B 72,32 Aqua the. 2.00 VP / Acrylates / Lauryl Methacrylate Copolymer

0.53 AMP

1, 00 Resilinprotein

0.20 Ceteareth-25

0.50 panthenol 0.05 benzophenone-4

0.20 Amodimethicone, Cetrimonium Chloride, Trideceth-12

15.00 Alcohol

C 0.20 hydroxyethylcellulose

D 6,00 Propane / Butane

WS 5%

% Ingredient (INCI)

A 2.00 cocotrimonium methosulfate q.s. perfume oil

B 68,32 Aqua the. 2.00 VP / Acrylates / Lauryl Methacrylate Copolymer

0.53 AMP

5.00 Resilinprotein

0.20 Ceteareth-25

0.50 panthenol 0.05 benzophenone-4

0.20 Amodimethicone, Cetrimonium Chloride, Trideceth-12 15.00 Alcohol

C 0.20 hydroxyethylcellulose

D 6,00 Propane / Butane

Preparation: Mix the components of phase A. Add the components of phase B one by one and dissolve. Dissolve phase C in the mixture of A and B, then adjust the pH to 6-7. Fill with phase D.

Example 22: Styling foam

WS 1%

% Ingredient (INCI)

A 2.00 Cetrimonium Chloride q.s. perfume oil

B 67,85 Aqua the. 7.00 Polyquaternium-46

1, 00 Resilinprotein

0.20 Ceteareth-25

0.50 panthenol

0.05 Benzophenone-4 0.20 Amodimethicone, Cetrimonium Chloride, Trideceth-12

15.00 Alcohol

C 0.20 hydroxyethylcellulose

D 6,00 Propane / Butane

WS 5%

% Ingredient (INCI)

A 2.00 Cetrimonium Chloride q.s. perfume oil

B 63,85 Aqua the.

7.00 Polyquaternium-46 5.00 Resilinprotein

0.20 Ceteareth-25

0.50 panthenol

0.05 benzophenone-4

0.20 Amodimethicone, Cetrimonium Chloride, Trideceth-12 15.00 Alcohol C 0.20 hydroxyethylcellulose

D 6,00 Propane / Butane

Example 23: Styling foam

WS 1%

% Ingredient (INCI)

A q. S. PEG-40 Hydrogenated Castor OiI q- S. Perfume oil

85, 5 Aqua the.

B 7.0 Sodium Polystyrene Sulfonate

1, 0 Resilinprotein 0.5 Cetrimonium Bromide q.s. preservative

C 6.0 propane / butane

Styling foam

WS 5%

% Ingredient (INCI)

A q.s. PEG-40 Hydrogenated Castor OiI q.s. Perfume oil 81, 5 Aqua dem.

B 7.0 Sodium Polystyrene Sulfonate 5.0 Resilin protein

0.5 Cetrimonium Bromide q.s. preservative

C 6.0 propane / butane

Preparation: Solubilize phase A. Weigh phase B into phase A and solve it clearly. Adjust the pH to 6-7, fill with phase C. Example 24: Styling foam

WS 1%

% Ingredient (INCI) A qs PEG-40 Hydrogenated Castor OiI qs perfume oil

92.0 Aqua the.

B 0.5 polyquaternium-10

1, 0 Resilinprotein

0.5 Cetrimonium Bromide q.s. preservative

C 6.0 propane / butane

WS 5%

% Ingredient (INCI)

A q.s. PEG-40 Hydrogenated Castor OiI q.s. perfume oil

88.0 Aqua the.

B 0.5 Polyquaternium-10 5.0 Resilinprotein

0.5 Cetrimonium Bromide q.s. preservative

C 6.0 propane / butane

Preparation: Solubilize phase A. Weigh phase B into phase A and solve it clearly. Adjust the pH to 6-7, fill with phase C.

Example 25: Styling foam

WS 1%

% Ingredient (INCI)

A q. S. PEG-40 Hydrogenated Castor OiI q- S. Perfume oil

82, 5 Aqua the.

B 10.0 Polyquaternium-16

1, 0 resilin protein 0.5 hydroxyethyl cetyldimonium phosphates q.s. preservative

C 6.0 propane / butane WS 5%

% Ingredient (INCI)

A q.s. PEG-40 Hydrogenated Castor OiI q.s. perfume oil

78.5 Aqua the.

B 10.0 Polyquaternium-16

5.0 Resilin Protein 0.5 Hydroxyethyl Cetyldimonium Phosphate q.s. preservative

C 6.0 propane / butane

Example 26: Styling foam

WS 1%

% Ingredient (INCI)

A 2.0 cocotrimony methosulfate q.s. perfume oil

B 84.0 Aqua dem.

2.0 Chitosan

1, 0 Resilinprotein

0.5 dimethicone copolyol 0.2 ceteareth-25

0.2 panthenol

0.1 PEG-25 PABA

C 10.0 HFC 152 A

WS 5%

% Ingredient (INCI)

A 2.0 cocotrimony methosulfate q.s. perfume oil

B 80.0 Aqua dem. 2.0 Chitosan

5.0 Resilinprotein

0.5 dimethicone copolyol

0.2 Ceteareth-25

0.2 panthenol

0.1 PEG-25 PABA

C 10.0 HFC 152 A

Example 27: care shampoo

WS 1%

% Ingredient (INCI)

A 30.0 Sodium Laureth Sulfate

6.0 Sodium Cocoamphoacetate 6.0 Cocamidopropyl Betaine

3.0 Sodium Laureth Sulfate, Glycol Distearate, Cocamide MEA, Laureth-10

1, 0 Resilinprotein

7.7 polyquaternium-44

2.0 Amodimethicone q.s. Perfume oil q.s. preservative

1, 0 Sodium Chloride

43.3 Aqua the.

B q.s. Citric Acid

WS 5%

% Ingredient (INCI)

A 30.0 Sodium Laureth Sulfate

6.0 Sodium Cocoamphoacetate

6.0 Cocamidopropyl Betaine

3.0 Sodium Laureth Sulfate, Glycol Distearate, Cocamide MEA, Laureth-10

5.0 Resilinprotein 7,7 Polyquaternium-44

2.0 Amodimethicone q.s. Perfume oil q.s. preservative

1, 0 Sodium Chloride 39.3 Aqua dem. B qs Citric Acid

Preparation: Mix and dissolve the components of phase A. Adjust the pH to 6-7 with citric acid.

Example 28: shower gel

WS 1%

% Ingredient (INCI)

A 40.0 Sodium Laureth Sulfate

5.0 decyl glucosides

5.0 Cocamidopropyl betaines

1, 0 Resilinprotein 1, 0 Panthenol q.s. Perfume oil q.s. preservative

2.0 Sodium Chloride

46.0 Aqua the.

B q.s. Citric Acid

WS 5%

% Ingredient (INCI)

A 40.0 Sodium Laureth Sulfate

5.0 decyl glucosides

5.0 Cocamidopropyl betaines

5.0 Resilinprotein 1, 0 Panthenol q.s. Perfume oil q.s. preservative

2.0 Sodium Chloride

42.0 Aqua the.

B q.s. Citric Acid

Example 29: Shampoo

WS 1%

% Ingredient (INCI)

A 40.0 Sodium Laureth Sulfate

5.0 Sodium C12-15 Pareth-15 Sulfonates 5.0 Decyl Glucoside qs perfume oil

0.1 phytantriol

44.6 Aqua the.

1, 0 Resilinprotein

0.3 polyquaternium-10

1, 0 panthenol q.s. preservative

1, 0 Laureth-3

2.0 Sodium Chloride

WS 5%

% Ingredient (INCI)

A 40.0 Sodium Laureth Sulfate

5.0 Sodium C12-15 Pareth-15 Sulfonates

5.0 Decyl Glucosides q.s. perfume oil

0.1 phytantriol

40.6 Aqua the.

5.0 Resilinprotein

0.3 polyquaternium-10

1, 0 panthenol q.s. preservative

1, 0 Laureth-3

2.0 Sodium Chloride

Example 30: Shampoo

WS 1%

% Ingredient (INCI)

A 15, 00 cocamidopropyl betaines

10, 00 Disodium Cocoamphodiacetate

5, 00 polysorbates 20

5.00 Decyl Glucoside q.S. Perfume oil q .S. preservative

1, 00 Resilinprotein

0.15 guar hydroxypropyltrimonium chlorides

2, 00 Laureth-3

58, 00 Aqua the. q .S. Citric Acid B 3.00 PEG-150 distearate

WS 5%

% Ingredient (INCI)

15, 00 cocamidopropyl betaines

10, 00 Disodium Cocoamphodiacetate

5, 00 polysorbates 20

5.00 Decyl Glucoside q.S. Perfume oil q .S. preservative

5, 00 Resilinprotein

0.15 guar hydroxypropyltrimonium chlorides

2, 00 Laureth-3

54, 00 Aqua the. q .S. Citric Acid

B 3.00 PEG-150 distearate

Production: Weigh in the components of phase A and dissolve. Adjust the pH to 6-7. Add phase B and heat to approx. 50 ^° C. Cool to room temperature while stirring.

Example 31: Moisturizing Body Care Cream

WS 1%

% Ingredient (INCI)

A 2.0 Ceteareth-25

2.0 Ceteareth-6, Stearyl Alcohol

3.0 Cetearyl ethylhexanoate

1, 0 dimethicone

4.0 Cetearyl Alcohol

3.0 Glyceryl Stearate SE

5.0 mineral oil

4.0 Simmondsia Chinensis (Jojoba) Seed OiI

3.0 Mineral OiI, Lanolin Alcohol

B 5.0 Propylene Glycol

1, 0 Resilinprotein

1, 0 panthenol

0.5 Mg Aluminum Silicate Q.s Preservative

65.5 Aqua the.

C qs perfume oil D qs Citric Acid

WS 5%

% Ingredient (INCI)

2.0 Ceteareth-25

2.0 Ceteareth-6, Stearyl Alcohol

3.0 Cetearyl ethylhexanoate

1, 0 dimethicone

4.0 Cetearyl Alcohol

3.0 Glyceryl Stearate SE

5.0 mineral oil

4.0 Simmondsia Chinensis (Jojoba) Seed OiI

3.0 Mineral OiI, Lanolin Alcohol

B 5.0 Propylene Glycol

5.0 Resilinprotein

1, 0 panthenol

0.5 Mg Aluminum Silicate Q.s Preservative

61, 5 Aqua the.

C q.s. perfume oil

D q.s. Citric Acid

Preparation: Heat phases A and B separately to about 80 ^° C. Phase B prehomogenising briefly, then stir phase B into phase A and again homogenisieren.Abkühlen to about 40 ⁰ C, Phase C and homogenize again good. Adjust the pH to 6-7 with citric acid.

Example 32: Moisturizing Body Care Cream

WS 1%% ingredient (INCI)

A 6.0 PEG-7 Hydrogenated Castor OiI

10.0 Cetearyl ethylhexanoates

5.0 Isopropyl myristate 7.0 Mineral OiI

0.5 Shea Butter (Butyrospermum Parkii)

0.5 Aluminum Stearate

0.5 mg stearate

0.2 Bisabolol 0.7 Quaternium 18 hectorite B 5.0 Dipropylene glycol

0.7 magnesium sulphates

1, 0 Resilinprotein q.s. preservative

62.9 Aqua.

C q.s. perfume oil

WS 5%

% Ingredient (INCI)

6.0 PEG-7 Hydrogenated Castor OiI

10.0 Cetearyl ethylhexanoates

5.0 isopropyl myristate

7.0 Mineral OiI

0.5 Shea Butter (Butyrospermum Parkii)

0.5 Aluminum Stearate

0.5 mg stearate

0.2 bisabolol

0.7 Quaternium-18 hectorites

B 5.0 Dipropylene glycol

0.7 magnesium sulphates

5.0 Resilin protein q.s. preservative

58.9 Aqua. q.s. perfume oil

Preparation: Heat phases A and B separately to about 80 ^° C. Bring phase B into phase A and homogenize. Cool with stirring to about 40 ⁰ C, add phase C and homogenize again. Allow to cool to room temperature while stirring.

Example 33: Liquid Make-up - Type O / W

WS 1%

% Ingredient (INCI)

A 2.0 Ceteareth-6, Stearyl Alcohol

2.0 Ceteareth-25

6.0 glyceryl stearates

1, 0 Cetyl Alcohol

8.0 mineral OiI

7.0 cetearyl ethylhexanoate

0.2 Dimethicone B 3.0 Propylene glycol

1, 0 panthenol q.s. preservative

1, 0 Resilinprotein

61, 9 Aqua the.

C 0.1 bisabolol q.s. perfume oil

D 5.7 C.I. 77 891, Titanium Dioxide

1, 1 Iran Oxides

WS 5%

% Ingredient (INCI)

A 2.0 Ceteareth-6, Stearyl Alcohol

2.0 Ceteareth-25

6.0 glyceryl stearates

1, 0 Cetyl Alcohol

8.0 mineral OiI

7.0 cetearyl ethylhexanoate

0.2 dimethicone

B 3.0 Propylene glycol

1, 0 panthenol

5.0 Resilin protein q.s. preservative

57.9 Aqua the.

C 0.1 bisabolol q.s. perfume oil

D 5.7 C.I. 77 891, Titanium Dioxide

1, 1 Iran Oxides

Preparation: Heat phases A and B separately to about 80 ^° C. Stir phase B into phase A and homogenize. Cool with stirring to about 40 ⁰ C, add phases C and D and thoroughly homogenize again. Allow to cool to room temperature while stirring.

Example 34

In the following, dermocosmetic preparations according to the invention are described, comprising the resilin protein prepared according to example 1. The following data are parts by weight of an aqueous solution. Clear shampoo

Clear conditioner shampoo

Foam O / W emulsions

Conditioner Shampoo with pearlescent Adjust pH to 6.0

Clear conditioner shampoo

Adjust pH to 6.0

Clear conditioner shampoo with volume effect

Adjust pH to 6.0

Gel Cream

OW Sunscreen formulation

Hydro dispersion

Sticks

PIT emulsion 100

Gel Cream

OW Formulations self-tanner

OW Make Up

Hydrodispersion self-tanner

Hydrodispersion after sun

WO-emulsion

Solid stabilized emulsion (Pickering emulsions)

Sticks Perfume oil 0.20 0.20 0.24 0.16 0.10 0.10

Aqua the. ad ad ad ad ad 100 ad ad 100 100 100 100 100 100 100

oil gel

Example 35:

The following formulations describe cosmetic sunscreen preparations comprising a combination of at least one inorganic pigment, preferably zinc oxide and / or titanium dioxide, and organic UV-A and UV-B filters.

The following formulations are prepared in a customary manner known to the person skilled in the art.

The content of Resilinprotein prepared according to Example 1 refers to 100% active ingredient. The active compounds according to the invention can be used both in pure form and as an aqueous solution. In the case of the aqueous solution, the content of water must be the. be adapted in the respective formulation.

0.10 allantoin allantoin

2% Resilinprotein

2.0 Mexoryl SX Terephthalidene Dicamphor Sulfonic Acid

65.20 water to the. Aqua the.

D 2.00 Simulge I NS hydroxyethyl acrylate / sodium acryloyl dimethyl taurate copolymer, squalane, polysorbate 60 q.s. preservative

Diethylamino hydroxybenzoyl hexyl benzoate

2.00 Uvinul N 539 T Octocrylene

B 5.00 T-Lite SF-S Titanium Dioxide, Silica Hydrate, Alumina Hydrate, Methicone / Dimethicone Copolymer

C 3.00 1, 2-propylene glycol Care Propylene Glycol

0.30 Abiol Imidazolidinyl urea

1, 00 magnesium sulfate 7-hydrate magnesium sulfates

0.5% Resilinprotein

Ad 100 water to the. Aqua the. q.s. preservative

Description of the pictures:

Fig. 1 Analysis of the purified Ui6 protein by SDS-PAGE: (A) Coomassie staining (B) detection with anti-T7 antibody after Western blot. The antibody binds the T7 peptide tag located at the N-terminus of the Ui6 repetitive sequence.

Lane 1: purified Ui6 protein; Lane 2: protein standard marker; MW: molecular weights of the corresponding marker bands.

Fig. 2 Analysis of the purity of the purified Ui6 by fluorescence spectroscopy. Ui6 does not contain the amino acid tryptophan while tyrosines are present. In contrast, tryptophan is present at an average frequency of 1.5% in E. coli proteins. Fluorescence spectroscopy can be used to selectively detect the presence of tryptophan in a protein sample and thus of impurities in Ui6 preparations. While tyrosines and tryptophans are excited by light of wavelength 280 nm, light at 295 nm leads exclusively to the stimulation of tryptophan. The Ui6 sample shows tyrosine-specific fluorescence, while no indication of the presence of tryptophan is detectable, indicating the high purity of the protein sample.

Claims

claims

1. Use of Resilinproteinen in cosmetics, characterized in that the Resilinproteine between one and 100 repeat units containing the consensus sequence SXXYGXP, where S is a serine, X is an arbitrary amino acid, Y is a tyrosine, G is a glycine and P is a proline.

2. Use according to claim 1, characterized in that the repeating units contain the following consensus sequence: in which

X ₄ is proline, alanine, threonine or serine S serine

X5 is a charged or polar amino acid

Xβ is a charged or polar amino acid

Y is a tyrosine

G is a glycine X7, a proline, alanine, threonine or serine

P a proline

Xe is a glycine, alanine, threonine or serine

Xg is a glycine a polar amino acid or no amino acid

X10 a glycine is a polar amino acid or no amino acid.

3. Use according to claim 1, characterized in that it is a hybrid protein consisting of Resilinsequenzen and sequences of non-Resilin structural proteins.

4. Use according to claim 1-3, characterized in that a Resi- linprotein is connected to a effector molecule.

5. Use according to claim 4, characterized in that the effector molecule is a UV absorber.

6. Use according to claim 1-5, characterized in that the Resi- linprotein is used in the skin cosmetics.

7. Cosmetic compositions containing at least one Resilinprotein and at least one active ingredient from the group of UV absorbers.

8. Basic Resilinproteine, characterized in that the ratio of the basic amino acid residues (K, R) to the acidic amino acid residues (D, E) contained in the repeating units which form the Resilinprotein at least 1, 5.

9. Basic Resilinproteine according to claim 8, characterized in that they have the structure SEQ ID NO: 1

10. A process for the preparation of basic Resilinproteinen according to claim 8, characterized in that a gene sequence coding for a repeat unit of a basic Resilinproteins is prepared, these repeating units are assembled to a total gene of the desired number of repeating units, the total gene in a host organism is expressed and the expressed basic Resilinprotein from the host organism or its culture medium is isolated.