EP1994175A4 - Solution pre-treatment for use in dna amplification reactions - Google Patents

Solution pre-treatment for use in dna amplification reactions

Info

Publication number
EP1994175A4
EP1994175A4 EP07747673A EP07747673A EP1994175A4 EP 1994175 A4 EP1994175 A4 EP 1994175A4 EP 07747673 A EP07747673 A EP 07747673A EP 07747673 A EP07747673 A EP 07747673A EP 1994175 A4 EP1994175 A4 EP 1994175A4
Authority
EP
European Patent Office
Prior art keywords
treatment
dna amplification
amplification reactions
solution pre
solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP07747673A
Other languages
German (de)
French (fr)
Other versions
EP1994175A1 (en
Inventor
Hugh William Morgan
Andreas Rueckert
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ZyGEM Corp Ltd
Original Assignee
WaikatoLink Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by WaikatoLink Ltd filed Critical WaikatoLink Ltd
Publication of EP1994175A1 publication Critical patent/EP1994175A1/en
Publication of EP1994175A4 publication Critical patent/EP1994175A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP07747673A 2006-03-10 2007-03-08 Solution pre-treatment for use in dna amplification reactions Withdrawn EP1994175A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
NZ54589406A NZ545894A (en) 2006-03-10 2006-03-10 Pre-treatment of DNA amplification solutions
PCT/NZ2007/000045 WO2007105965A1 (en) 2006-03-10 2007-03-08 Solution pre-treatment for use in dna amplification reactions

Publications (2)

Publication Number Publication Date
EP1994175A1 EP1994175A1 (en) 2008-11-26
EP1994175A4 true EP1994175A4 (en) 2009-12-23

Family

ID=38509721

Family Applications (1)

Application Number Title Priority Date Filing Date
EP07747673A Withdrawn EP1994175A4 (en) 2006-03-10 2007-03-08 Solution pre-treatment for use in dna amplification reactions

Country Status (6)

Country Link
EP (1) EP1994175A4 (en)
JP (1) JP2009529346A (en)
CN (1) CN101432443A (en)
AU (1) AU2007225509A1 (en)
NZ (1) NZ545894A (en)
WO (1) WO2007105965A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8507662B2 (en) 2001-01-19 2013-08-13 General Electric Company Methods and kits for reducing non-specific nucleic acid amplification
US8361712B2 (en) * 2007-12-17 2013-01-29 General Electric Company Contamination-free reagents for nucleic acid amplification
US8198040B2 (en) 2006-02-28 2012-06-12 Montana State University Use of phenanthridium derivatives for distinguishing between live and dead cells

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6379930B1 (en) * 1999-07-30 2002-04-30 Applied Gene Technologies, Inc. Stabilization of nucleic acid amplification cocktails

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5532145A (en) * 1989-10-26 1996-07-02 Steritech, Inc. Methods for treatment of enzyme preparations
US6187566B1 (en) * 1999-03-09 2001-02-13 Applied Gene Technologies, Inc. Method of labeling a nucleic acid amplicon with simultaneous contamination prevention
EP1627926A1 (en) * 2004-08-19 2006-02-22 Roche Diagnostics GmbH A method to reduce false positive results

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6379930B1 (en) * 1999-07-30 2002-04-30 Applied Gene Technologies, Inc. Stabilization of nucleic acid amplification cocktails

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
NOCKER ANDREAS ET AL: "Selective removal of DNA from dead cells of mixed bacterial communities by use of ethidium monoazide", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 72, no. 3, 1 March 2006 (2006-03-01), pages 1997 - 2004, XP002541184, ISSN: 0099-2240 *
NOGVA HEGE KARIN ET AL: "Ethidium monoazide for DNA-based differentiation of viable and dead bacteria by 5'-nuclease PCR", BIOTECHNIQUES, INFORMA LIFE SCIENCES PUBLISHING, WESTBOROUGH, MA, US, vol. 34, no. 4, 1 April 2003 (2003-04-01), pages 804 - 813, XP008109791, ISSN: 0736-6205 *
RUDI K ET AL: "Detection of viable and dead Listeria monocytogenes on gouda-like cheeses by real-time PCR Comments : Real-time diagnostics to detect the presence of viable, dead or viable but not culturable cells", LETTERS IN APPLIED MICROBIOLOGY, OXFORD, GB, vol. 40, no. 4, 1 January 2005 (2005-01-01), pages 301 - 306, XP002482493, ISSN: 1472-765X *
RUDI K ET AL: "Use of ethidium monoazide and PCR in combination for quantification of viable and dead cells in complex samples", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 71, no. 2, 1 January 2005 (2005-01-01), pages 1018 - 1024, XP003000006, ISSN: 0099-2240 *
RUECKERT A ET AL: "Rapid differentiation and enumeration of the total, viable vegetative cell and spore content of thermophilic bacilli in milk powders with reference to Anoxybacillus flavithermus", JOURNAL OF APPLIED MICROBIOLOGY, vol. 99, no. 5, 2005, pages 1246 - 1255, XP008109747, ISSN: 1364-5072 *
See also references of WO2007105965A1 *
WANG S ET AL: "Discrimination of viable Vibrio vulnificus cells from dead cells in real-time PCR", JOURNAL OF MICROBIOLOGICAL METHODS, ELSEVIER, AMSTERDAM, NL, vol. 64, no. 1, 1 January 2006 (2006-01-01), pages 1 - 8, XP025072992, ISSN: 0167-7012, [retrieved on 20060101], DOI: 10.1016/J.MIMET.2005.04.023 *

Also Published As

Publication number Publication date
EP1994175A1 (en) 2008-11-26
NZ545894A (en) 2007-12-21
WO2007105965A1 (en) 2007-09-20
JP2009529346A (en) 2009-08-20
AU2007225509A1 (en) 2007-09-20
CN101432443A (en) 2009-05-13

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Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20080925

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC MT NL PL PT RO SE SI SK TR

A4 Supplementary search report drawn up and despatched

Effective date: 20091120

17Q First examination report despatched

Effective date: 20100406

RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: ZYGEM CORPORATION LIMITED

DAX Request for extension of the european patent (deleted)
STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20120829