EP1956087A4 - High production system for infectious hepatitis c virus particle - Google Patents

High production system for infectious hepatitis c virus particle

Info

Publication number
EP1956087A4
EP1956087A4 EP06810936A EP06810936A EP1956087A4 EP 1956087 A4 EP1956087 A4 EP 1956087A4 EP 06810936 A EP06810936 A EP 06810936A EP 06810936 A EP06810936 A EP 06810936A EP 1956087 A4 EP1956087 A4 EP 1956087A4
Authority
EP
European Patent Office
Prior art keywords
hcv
infectious hepatitis
production system
high production
virus particle
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP06810936A
Other languages
German (de)
French (fr)
Japanese (ja)
Other versions
EP1956087B1 (en
EP1956087A1 (en
Inventor
Jun-Ichi Tanabe
Saburo Sone
Takaji Wakita
Koji Ishii
Ryosuke Suzuki
Tetsuro Suzuki
Tatsuo Miyamura
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
JAPAN AS REPRESENTED BY DIRECTOR GENERAL OF NATION
Tokyo Metropolitan Organization for Medical Research
Toray Industries Inc
Original Assignee
National Institute of Infectious Diseases
Tokyo Metropolitan Organization for Medical Research
Toray Industries Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National Institute of Infectious Diseases, Tokyo Metropolitan Organization for Medical Research, Toray Industries Inc filed Critical National Institute of Infectious Diseases
Publication of EP1956087A1 publication Critical patent/EP1956087A1/en
Publication of EP1956087A4 publication Critical patent/EP1956087A4/en
Application granted granted Critical
Publication of EP1956087B1 publication Critical patent/EP1956087B1/en
Not-in-force legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/24011—Flaviviridae
    • C12N2770/24211—Hepacivirus, e.g. hepatitis C virus, hepatitis G virus
    • C12N2770/24241—Use of virus, viral particle or viral elements as a vector
    • C12N2770/24243—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/24011—Flaviviridae
    • C12N2770/24211—Hepacivirus, e.g. hepatitis C virus, hepatitis G virus
    • C12N2770/24251—Methods of production or purification of viral material
    • C12N2770/24252—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The invention relates to a method for producing an infectious hepatitis C virus (HCV) particle, comprising the step of introducing an expression vector containing a DNA fragment including a DNA sequence encoding a 5’ noncoding region and a structural protein of HCV and an arbitrary nonstructural protein and a DNA sequence encoding a nonstructural protein and a 3’ noncoding region derived from HCV JFH1 strain downstream of the RNA polymerase I promoter and further containing a DNA fragment including the RNA polymerase I terminator downstream thereof into a cell which allows HCV proliferation.
EP06810936A 2005-09-30 2006-09-29 High production system for infectious hepatitis c virus particle Not-in-force EP1956087B1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2005287646 2005-09-30
PCT/JP2006/319572 WO2007037428A1 (en) 2005-09-30 2006-09-29 High production system for infectious hepatitis c virus particle

Publications (3)

Publication Number Publication Date
EP1956087A1 EP1956087A1 (en) 2008-08-13
EP1956087A4 true EP1956087A4 (en) 2009-05-27
EP1956087B1 EP1956087B1 (en) 2010-05-26

Family

ID=37899848

Family Applications (1)

Application Number Title Priority Date Filing Date
EP06810936A Not-in-force EP1956087B1 (en) 2005-09-30 2006-09-29 High production system for infectious hepatitis c virus particle

Country Status (10)

Country Link
US (1) US8143022B2 (en)
EP (1) EP1956087B1 (en)
JP (1) JP5030065B2 (en)
KR (1) KR101355903B1 (en)
CN (1) CN101321865B (en)
AU (1) AU2006295800B2 (en)
CA (1) CA2624242A1 (en)
DE (1) DE602006014586D1 (en)
ES (1) ES2341800T3 (en)
WO (1) WO2007037428A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101835896A (en) 2007-07-13 2010-09-15 日本国立感染症研究所 Production and use of epitope-tagged hepatitis c virus particle
WO2009131203A1 (en) 2008-04-25 2009-10-29 東レ株式会社 Nucleic acid containing chimeric gene derived from hepatitis type-c virus
US8834893B2 (en) 2008-12-26 2014-09-16 Toray Industries, Inc. Nucleic acid derived from hepatitis C virus and expression vector, transformed cell, and hepatitis C virus particles each prepared by using the same
CN102596996B (en) * 2009-10-30 2014-06-18 东丽株式会社 Antibody having activity of inhibiting infection with hepatitis c virus (HCV) and use of same

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19915178A1 (en) 1999-04-03 2000-10-05 Univ Mainz Johannes Gutenberg Hepatitis C virus cell culture system
ES2347237T3 (en) 2003-05-26 2010-10-27 Toray Industries, Inc. NUCLEIC ACID CONSTRUCTION CONTAINING THE HEPATITIS C (HCV) VIRUS OF THE NUCLEIC ACID OF GENOMIC ORIGIN OF GENOTIPO 2A AND CELL THAT HAS THE NUCLEIC ACID CONSTRUCTION TRANSFERRED TO THE INSIDE.
ES2366303T3 (en) 2004-02-20 2011-10-19 Tokyo Metropolitan Organization For Medical Research CONSTRUCTION OF NUCLEIC ACID CONTAINING THE COMPLETE LENGTH GENOMA OF THE HUMAN HEPATITIS C VIRUS, CELLS THAT REPLICATE THE GENOMA OF THE COMPLETE LENGTH VIRUS THAT HAVE THE CONSTRUCTION OF TRANSFERRED NUCLEIC ACID AND PRODUCTION PROCEDURE OF THE PARTITION OF THE PARTITION OF THE PARTITION.

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
HELLER THEO ET AL: "An in vitro model of hepatitis C virion production", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 102, no. 7, 8 February 2005 (2005-02-08), pages 2579 - 2583, XP009079485, ISSN: 0027-8424 *
See also references of WO2007037428A1 *
WAKITA T ET AL: "Production of infectious hepatitis C virus in tissue culture from a cloned viral genome", NATURE MEDICINE, vol. 11, no. 7, 1 July 2005 (2005-07-01), pages 791 - 796, XP002993378 *

Also Published As

Publication number Publication date
WO2007037428A1 (en) 2007-04-05
EP1956087B1 (en) 2010-05-26
US8143022B2 (en) 2012-03-27
CN101321865B (en) 2011-12-14
JPWO2007037428A1 (en) 2009-04-16
KR20080068817A (en) 2008-07-24
ES2341800T3 (en) 2010-06-28
EP1956087A1 (en) 2008-08-13
US20100035345A1 (en) 2010-02-11
CA2624242A1 (en) 2007-04-05
AU2006295800B2 (en) 2011-08-18
JP5030065B2 (en) 2012-09-19
KR101355903B1 (en) 2014-01-28
AU2006295800A1 (en) 2007-04-05
CN101321865A (en) 2008-12-10
DE602006014586D1 (en) 2010-07-08

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