EP1846165B1 - Maldi chip holder - Google Patents

Maldi chip holder Download PDF

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Publication number
EP1846165B1
EP1846165B1 EP06707939.2A EP06707939A EP1846165B1 EP 1846165 B1 EP1846165 B1 EP 1846165B1 EP 06707939 A EP06707939 A EP 06707939A EP 1846165 B1 EP1846165 B1 EP 1846165B1
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EP
European Patent Office
Prior art keywords
holder
maldi
chip
maldi chip
slider
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Not-in-force
Application number
EP06707939.2A
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German (de)
French (fr)
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EP1846165A2 (en
Inventor
Karsten Reihs
Udo Adels
Guido Steffen
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Qiagen GmbH
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Qiagen GmbH
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Publication of EP1846165A2 publication Critical patent/EP1846165A2/en
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    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/02—Details
    • H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
    • H01J49/0409—Sample holders or containers
    • H01J49/0418—Sample holders or containers for laser desorption, e.g. matrix-assisted laser desorption/ionisation [MALDI] plates or surface enhanced laser desorption/ionisation [SELDI] plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/52—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
    • B01L9/523—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips for multisample carriers, e.g. used for microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0819—Microarrays; Biochips

Definitions

  • the present invention relates to a holder for a MALDI chip, which has a broken corner contour, with a first and a second respectively chamfered contact surface and a movable fixing means, which cooperates with the broken corner contour and biases the MALDI chip against the contact surfaces. Furthermore, the present invention relates to a MALDI chip and a system consisting of the holder and the MALDI chip.
  • mass spectrometry For the analysis of samples, for example in drug chemistry or in biological research and production, mass spectrometry has become increasingly popular. For analysis of biomolecules in the samples, mass spectrometry with ionization by matrix-assisted laser desorption and ionization (MALDI) is preferably used.
  • MALDI matrix-assisted laser desorption and ionization
  • MALDI chip - In the MALDI method, in some cases more than 200 MALDI matrix points are applied to a sample carrier - referred to below as a MALDI chip - which then has to be analyzed. It will be understood by those skilled in the art that both the application of the samples and the later analysis, due to the plurality of MALDI matrix points arranged in a comparatively small space, requires very high precision and very precise positioning of the MALDI chip.
  • a MALDI chip can be positioned very precisely and reproducibly with the holder according to the invention, so that the metering of the substances to be analyzed and / or the subsequent analyzes can be carried out very precisely.
  • the holder according to the invention is simple and inexpensive to manufacture and use. Even deposits on the holder do not affect the exact position of the MALDI chips.
  • the holder is suitable for holding MALDI chips with a broken, i. flattened corner contour.
  • the holder has a first and a second contact surface, which are preferably arranged at right angles to one another and against which the MALDI chip rests. Furthermore, the holder according to the invention has a fixing means with which the MALDI chip can be fixed in contact with the contact surfaces. According to the MALDI chip is biased by the fixing means against the contact surfaces. This has the advantage that even with mechanical influences such as vibrations or temperature influences, the MALDI chip is permanently in contact with the first and the second contact surface, so that its position in a plane is at least clearly defined. Furthermore, according to the invention, the fixing agent cooperates with the broken corner contour.
  • the holder has a support surface on which rests the MALDI chip.
  • the contact surfaces of the holder according to the invention are chamfered such that the MALDI chip, in particular, when it is under pretension, is biased against the support. This has the advantage that the spatial position of the MALDI chip on the holder according to the invention is uniquely determined and the MALDI chip is always held in a unique position.
  • the fixing means also has a contact surface, which cooperates with the MALDI chip and is preferably complementary to a corner contour of the MALDI chip.
  • this contact surface is chamfered, so that the MALDI chip is also pressed by this contact surface against the support surface of the holder.
  • the fixative is a slider.
  • this slider has a spring which biases him in the direction of the contact surfaces.
  • At least one contact surface of the holder is at least partially coated with a highly electrically conductive material, which is particularly preferably not corroded.
  • the material is gold.
  • the support surface has a recess, in particular a bore. This hole facilitates removal of the MALDI chip after analysis.
  • one or more spring means is arranged on the opposite side of the support surface of the holder, with which the holder relative to the analyzer is biased.
  • the holder has a lever which is reversibly convertible from a locking to an unlocking position.
  • the lever is provided detent in both positions.
  • the lever In the locked position, the lever preferably conceals the recess in the support surface and continues to block prefers the fixative. Both measures prevent the MALDI chip from being unintentionally removed from the holder.
  • the opening in the support surface In the unlocked position, the opening in the support surface is freely accessible and the fixing means unlocked, so that the MALDI chip is detachable from the holder.
  • Another object of the present invention is a Maldi chip having a gold-plated edge region.
  • the gold-plated edge region and the analysis region are connected to one another by a conductor track.
  • Yet another object of the present invention is a system consisting of the holder according to the invention and the Maldi chip according to the invention.
  • FIG. 1 shows a MALDI chip 6 having a plurality of points 12 to which a MALDI matrix can be dosed.
  • the MALDI chip has its upper left Corner a flattened corner contour 8, wherein the skilled artisan recognizes that the corner contour can also be designed differently.
  • FIG. 2a shows the holder 1 according to the invention in the MALDI chip fixing state, the MALDI chip has been omitted for illustrative purposes.
  • the holder 1 has a first and a second contact surface 2, 3, on which the MALDI chip 6 (not shown in this figure) is applied. Between the contact surfaces 2, 3, a recess 10 is arranged, which is required for a processing of the contact surfaces 2, 3, but also prevents the MALDI chip is applied only at its corner.
  • the holder according to the invention has a support surface 5, on which the MALDI chip 6 rests.
  • the slide 4 which is movable according to the directions indicated by the double arrow 9 and which is biased by a spring (not shown) in the direction of the contact surfaces 2.3, the MALDI chip is pressed against the contact surfaces 2.3.
  • the upper illustration shows the slider from above ie from the side where the MALDI chip rests.
  • the slider 4 is located in its front, ie in a position fixing the MALDI chip.
  • the recess 15 can be seen in the support surface 5, which is covered in the MALDI chip fixing state from the rear of the lever 14.
  • the bottom view shows the holder 1 from below.
  • the lever 14 abuts against the holder and conceals the recess 15.
  • the lever prevents on the one hand, that an object is inserted through the recess 15, which presses the MALDI chip from its fixation. Furthermore, the slider 4 is blocked by the lever 14 so that it can not be transferred to a retracted position.
  • FIG. 2b shows the holder according to FIG. 2a in the rear unlocking position of the slide, in which the MALDI chip can be removed or a new MALDI chip can be inserted into the holder.
  • the lever 14 is arranged in this position substantially perpendicular to the holder and thereby releases the opening 15. Furthermore, the locking of the slider 4 is released by the lever.
  • FIG. 3 shows the MALDI chip 6 in its exactly fixed position.
  • the abutment surfaces 2, 3 are chamfered, so that the Spring force of the fixing means 4 to the contact surfaces 2.3 also induces a downward, symbolized by the arrow 11 movement, with which the MALDI chip 6 is permanently held in contact with the support surface 5, thereby ensuring that the MALDI chip. 6 also spatially clearly positioned on the holder.
  • FIG. 4 shows the slider of the inventive holder, which has a contact surface 7, which corresponds to the corner contour 8 of the MALDI chip 6. Also, the contact surface 7 is chamfered, so that the MALDI chip is also pressed by this surface against the support surface.
  • the lever 14, which is shown here in the locking position, does not touch the slider 4 in this position. The slider 4 has contact in this position with the corner contour 8 of the MALDI chip 6 and presses it against the contact surfaces 2, 3.
  • FIG. 5 shows how a MALDI chip is inserted into the holder according to the invention.
  • the slider 4 is first moved to the bottom right and locked in this position. Thereafter, the MALDI chip is inserted into the holder so that the flattened corner contour 8 points in the direction of the slider.
  • the slider is folded over and presses due to the force of the existing spring (not shown) the MALDI chip in the upper left corner that it rests against the contact surfaces 2, 3.
  • FIG. 6 shows the underside of the holder, the leaf springs 13 which bias the holder against the analyzer and ensure good electrical contact with the analyzer.

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  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Electron Beam Exposure (AREA)
  • Container, Conveyance, Adherence, Positioning, Of Wafer (AREA)

Description

Die vorliegende Erfindung betrifft einen Halter für einen MALDI-Chip, der eine gebrochenen Eckkontur aufweist, mit einer ersten und einer zweiten jeweils angefasten Anlagefläche und einem beweglichen Fixierungsmittel, das mit der gebrochenen Eckkontur zusammenwirkt und das den MALDI-Chip gegen die Anlageflächen vorspannt. Des weiteren betrifft die vorliegende Erfindung ein MALDI-Chip und ein System bestehend aus dem Halter und dem MALDI-Chip.The present invention relates to a holder for a MALDI chip, which has a broken corner contour, with a first and a second respectively chamfered contact surface and a movable fixing means, which cooperates with the broken corner contour and biases the MALDI chip against the contact surfaces. Furthermore, the present invention relates to a MALDI chip and a system consisting of the holder and the MALDI chip.

Für die Analyse von Proben beispielsweise in der Wirkstoffchemie oder in der biologischen Forschung und Produktion hat sich vermehrt die Massenspektrometrie durchgesetzt. Für die Analyse von in den Proben befindlichen Biomolekülen wird vorzugsweise die Massenspektrometrie mit Ionisierung durch Matrix-unterstützte Laserdesorption und Ionisierung (MALDI) eingesetzt.For the analysis of samples, for example in drug chemistry or in biological research and production, mass spectrometry has become increasingly popular. For analysis of biomolecules in the samples, mass spectrometry with ionization by matrix-assisted laser desorption and ionization (MALDI) is preferably used.

Bei dem MALDI-Verfahren werden insbesondere Biomoleküle und/oder biologisches Material in Form eines Flüssigkeitstropfens auf einen sogenannten MALDI-Matrix-Punkt dosiert, beispielsweise pipettiert, und getrocknet. Die sich dabei bildenden Kristalle werden beispielsweise mit einem MALDI-TOF Massenspektrometer im linearen- oder im Reflektor-Betrieb untersucht. Details zu diesem Verfahren können Nordhoff et. al. " MALDI-MS as a new method for the analysis of nucleic acid (DNA and RNA) with molecular masses up to 150,000 Dalton, Application of modern mass spectrometric methods to plant science research, Oxford University press, (1996 ) Seite 86- 101 entnommen werden, das hiermit als Referenz eingeführt wird und somit als Teil der Offenbarung gilt.In the MALDI method in particular biomolecules and / or biological material in the form of a liquid drop are metered to a so-called MALDI matrix point, for example pipetted, and dried. The resulting crystals are analyzed, for example, with a MALDI-TOF mass spectrometer in linear or in reflector operation. Details about this procedure can be Nordhoff et. al. "MALDI-MS as a new method for the analysis of nucleic acids (DNA and RNA) with molecular mass up to 150,000 daltons, Application of modern mass spectrometric methods to plan science research, Oxford university press, (1996 ) Page 86-101, which is hereby incorporated by reference and thus forms part of the disclosure.

Bei dem MALDI-Verfahren werden teilweise mehr als 200 MALDI-Matrixpunkte auf einen Probenträger - im folgenden als MALDI-Chip bezeichnet - aufgebracht, die dann analysiert werden müssen. Der Fachmann versteht, dass sowohl das Aufbringen der Proben als auch die spätere Analyse aufgrund der Vielzahl von MALDI-Matrixpunkten, die auf einem vergleichsweise kleinen Raum angeordnet sind, eine sehr hohe Präzision u.a eine sehr präzise Positionierung des MALDI-Chips erfordert.In the MALDI method, in some cases more than 200 MALDI matrix points are applied to a sample carrier - referred to below as a MALDI chip - which then has to be analyzed. It will be understood by those skilled in the art that both the application of the samples and the later analysis, due to the plurality of MALDI matrix points arranged in a comparatively small space, requires very high precision and very precise positioning of the MALDI chip.

Es war daher die Aufgabe der vorliegenden Neuerung, einen Halter zur Verfügung zu stellen, auf dem ein MALDI-Chip sehr exakt und reproduzierbar positioniert werden kann.It was therefore the object of the present innovation to provide a holder on which a MALDI chip can be positioned very accurately and reproducibly.

Gelöst wird die Aufgabe mit einem Halter gemäß'Schutzanspruch 1. Bevorzugte Ausführungsform des Erfindungsgemäßen Halters sind in den Ansprüchen 2 bis 11 beansprucht.The object is achieved with a holder according to the 'protection claim 1. Preferred embodiment of the inventive holder are claimed in claims 2 to 11.

Es war für den Fachmann überaus erstaunlich und nicht zu erwarten, dass mit dem erfindungsgemäßen Halter ein MALDI-Chip sehr exakt und reproduzierbar positioniert werden kann, so dass die Dosierung der zu analysierenden Substanzen und/oder die nachfolgenden Analysen sehr exakt durchgeführt werden können. Der erfindungsgemäße Halter ist einfach und kostengünstig herzustellen und einzusetzen. Selbst durch Ablagerungen auf dem Halter wird die exakte Lage der MALDI-Chips nicht beeinträchtigt.It was extremely surprising for the person skilled in the art and not to be expected that a MALDI chip can be positioned very precisely and reproducibly with the holder according to the invention, so that the metering of the substances to be analyzed and / or the subsequent analyzes can be carried out very precisely. The holder according to the invention is simple and inexpensive to manufacture and use. Even deposits on the holder do not affect the exact position of the MALDI chips.

Der Halter eignet sich zur Halterung von MALDI-Chips mit einer gebrochenen, d.h. abgeflachten Eckkontur.The holder is suitable for holding MALDI chips with a broken, i. flattened corner contour.

Erfindungsgemäß weist der Halter eine erste und eine zweite Anlagefläche auf, die vorzugsweise rechtwinklig zueinander angeordnet sind und an denen der MALDI-Chip anliegt. Des weiteren weist der erfindungsgemäße Halter ein Fixierungsmittel auf, mit dem der MALDI-Chip in Anlage mit den Anlageflächen fixierbar ist. Erfindungsgemäß wird der MALDI-Chip durch das Fixierungsmittel gegen die Anlageflächen vorgespannt. Dies hat den Vorteil, dass sich selbst bei mechanischen Einflüssen wie beispielsweise Vibrationen oder Temperatureinflüssen, der MALDI-Chip permanent in Anlage mit der ersten und der zweiten Anlagefläche befindet, so dass seine Position in einer Ebene zumindest eindeutig definiert ist. Weiterhin erfindungsgemäß wirkt das Fixierungsmittel mit der gebrochenen Eckkontur zusammen.According to the invention, the holder has a first and a second contact surface, which are preferably arranged at right angles to one another and against which the MALDI chip rests. Furthermore, the holder according to the invention has a fixing means with which the MALDI chip can be fixed in contact with the contact surfaces. According to the MALDI chip is biased by the fixing means against the contact surfaces. This has the advantage that even with mechanical influences such as vibrations or temperature influences, the MALDI chip is permanently in contact with the first and the second contact surface, so that its position in a plane is at least clearly defined. Furthermore, according to the invention, the fixing agent cooperates with the broken corner contour.

Der Halter weist eine Auflagefläche auf, auf der der MALDI-Chip aufliegt.The holder has a support surface on which rests the MALDI chip.

Die Anlageflächen sind des erfindungsgemäßen Halters so angefast, dass der MALDI-Chip, insbesondere, wenn er unter Vorspannung steht, gegen die Auflage vorgespannt ist. Dies hat den Vorteil, dass die räumliche Position des MALDI-Chips auf dem Erfindungsgemäßen Halter eindeutig bestimmt ist und der MALDI-Chip immer in einer eindeutigen Position gehalten wird.The contact surfaces of the holder according to the invention are chamfered such that the MALDI chip, in particular, when it is under pretension, is biased against the support. This has the advantage that the spatial position of the MALDI chip on the holder according to the invention is uniquely determined and the MALDI chip is always held in a unique position.

In einer bevorzugten Ausführungsform des erfindungsgemäßen Halters weist auch das Fixierungsmittel eine Anlagefläche auf, die mit dem MALDI-Chip zusammenwirkt und vorzugsweise komplementär zu einer Eckkontur des MALDI-Chips ist. Vorzugsweise ist auch diese Anlagefläche angefast, so dass der MALDI-Chip auch von dieser Anlagefläche gegen die Auflagefläche des Halters gedrückt wird.In a preferred embodiment of the holder according to the invention, the fixing means also has a contact surface, which cooperates with the MALDI chip and is preferably complementary to a corner contour of the MALDI chip. Preferably, this contact surface is chamfered, so that the MALDI chip is also pressed by this contact surface against the support surface of the holder.

Als Fixierungsmittel eignet sich jedes dem Fachmann geläufige Fixierungsmittel. Vorzugsweise handelt es sich bei dem Fixierungsmittel jedoch um einen Schieber. Vorzugsweise weist dieser Schieber eine Feder auf, die ihn in Richtung der Anlageflächen vorspannt.Any suitable fixing agent is known as a fixing agent. Preferably, however, the fixative is a slider. Preferably, this slider has a spring which biases him in the direction of the contact surfaces.

Vorzugsweise ist mindestens eine Anlagefläche des Halters mindestens teilweise mit einem elektrisch sehr gut leitenden Material, das besonders bevorzugt nicht korrodiert überzogen. Beispielweise handelt es sich bei dem Material um Gold.Preferably, at least one contact surface of the holder is at least partially coated with a highly electrically conductive material, which is particularly preferably not corroded. For example, the material is gold.

In einer weiteren bevorzugten Ausführungsform der vorliegenden Erfindung weist die Auflagefläche eine Ausnehmung, insbesondere eine Bohrung, auf. Durch diese Bohrung wird das Entfernen des MALDI-Chips nach erfolgter Analyse erleichtert.In a further preferred embodiment of the present invention, the support surface has a recess, in particular a bore. This hole facilitates removal of the MALDI chip after analysis.

Weiterhin bevorzugt ist an der der Auflagefläche gegenüberliegenden Seite des Halters ein oder mehrere Federmittel angeordnet, mit denen der Halter gegenüber dem Analysengerät vorspannbar ist.Further preferably, one or more spring means is arranged on the opposite side of the support surface of the holder, with which the holder relative to the analyzer is biased.

Außerdem bevorzugt weist der Halter einen Hebel auf, der von einer verriegelnden in eine entriegelnde Stellung reversibel überführbar ist. Vorzugsweise ist der Hebel in beiden Stellungen rastend vorgesehen. In der verriegelten Stellung verdeckt der Hebel vorzugsweise die Ausnehmung in der Auflagefläche und blockiert weiterhin bevorzugt das Fixierungsmittel. Beide Maßnahmen verhindern, dass der MALDI-Chip ungewollter weise aus dem Halter entfernt wird. In der entriegelten Stellung ist die Öffnung in der Auflagefläche frei zugänglich und das Fixierungsmittel entriegelt, so dass der MALDI-Chip von dem Halter lösbar ist.In addition, preferably, the holder has a lever which is reversibly convertible from a locking to an unlocking position. Preferably, the lever is provided detent in both positions. In the locked position, the lever preferably conceals the recess in the support surface and continues to block prefers the fixative. Both measures prevent the MALDI chip from being unintentionally removed from the holder. In the unlocked position, the opening in the support surface is freely accessible and the fixing means unlocked, so that the MALDI chip is detachable from the holder.

Ein weiterer Gegenstand der vorliegenden Erfindung ist ein Maldi-Chip aufweisend einen vergoldeten Randbereich.Another object of the present invention is a Maldi chip having a gold-plated edge region.

Vorzugsweise sind der vergoldete Randbereich und der Analysenbereich durch eine Leiterbahn miteinander verbunden.Preferably, the gold-plated edge region and the analysis region are connected to one another by a conductor track.

Ein noch weiterer Gegenstand der vorliegenden Erfindung ist ein System bestehend aus dem erfindungsgemäßen Halter und dem erfindungsgemäßen Maldi-Chip.Yet another object of the present invention is a system consisting of the holder according to the invention and the Maldi chip according to the invention.

Im Folgenden wird die Neuerung anhand der Figuren 1 bis 6 erläutert. Diese Erläuterungen sind lediglich beispielhaft und schränken den allgemeinen Neuerungsgedanken nicht ein.

  • Figur 1 zeigt schematisch einen MALDI-Chip.
  • Figuren 2a und 2b zeigen jeweils den erfindungsgemäßen Halter in zwei Zuständen.
  • Figur 3 zeigt einen Schnitt durch den erfindungsgemäßen Halter.
  • Figur 4 zeigt den Schieber des erfindungsgemäßen Halters im Zusammenspiel mit dem Hebel.
  • Figur 5 zeigt den erfindungsgemäßen Halter mit einem MALDI-Chip.
  • Figur 6 zeigt die Unterseite des Halters.
The following is the innovation based on the FIGS. 1 to 6 explained. These explanations are merely exemplary and do not limit the general idea of innovation.
  • FIG. 1 schematically shows a MALDI chip.
  • FIGS. 2a and 2 B each show the holder according to the invention in two states.
  • FIG. 3 shows a section through the holder according to the invention.
  • FIG. 4 shows the slider of the holder according to the invention in interaction with the lever.
  • FIG. 5 shows the holder according to the invention with a MALDI chip.
  • FIG. 6 shows the bottom of the holder.

Figur 1 zeigt einen MALDI-Chip 6, der eine Vielzahl von Punkten 12 aufweist, auf die eine MALDI-Matrix dosierbar ist. Der MALDI-Chip weist an seiner oberen linken Ecke eine abgeflachte Eckkontur 8 auf, wobei der Fachmann erkennt, das die Eckkontur auch anders gestaltet sein kann. FIG. 1 shows a MALDI chip 6 having a plurality of points 12 to which a MALDI matrix can be dosed. The MALDI chip has its upper left Corner a flattened corner contour 8, wherein the skilled artisan recognizes that the corner contour can also be designed differently.

Figur 2a zeigt den erfindungsgemäßen Halter 1 im den MALDI-Chip fixierenden Zustand, wobei der MALDI-Chip aus Anschauungsgründen weggelassen wurde. Der Halter 1 weist eine erste und eine zweite Anlagefläche 2, 3 auf, an denen der MALDI-Chip 6 (in dieser Figur nicht dargestellt) anliegt. Zwischen den Anlageflächen 2, 3 ist eine Ausnehmung 10 angeordnet, die zum einen zur Bearbeitung der Anlageflächen 2, 3 benötigt wird, die aber auch verhindert, dass der MALDI-Chip lediglich an seinem Eckpunkt anliegt. Des weiteren weist die erfindungsgemäße Halterung eine Auflagefläche 5 auf, auf der der MALDI-Chip 6 aufliegt. Mittels des Schiebers 4, der gemäß der durch den Doppelpfeil 9 gekennzeichneten Richtungen bewegbar ist und der mit einer Feder (nicht dargestellt) in Richtung der Anlageflächen 2,3 vorgespannt ist, wird der MALDI-Chip gegen die Anlageflächen 2,3 gedrückt. Durch die Anlage des MALDI-Chips an den Anlageflächen 2,3 ist seine Lage in der Papierebene eindeutig bestimmt. Die obere Darstellung zeigt den Schieber von oben d.h von der Seite auf der der MALDI-Chip aufliegt. Der Schieber 4 befindet sich in seiner vorderen d.h. in einer den MALDI-Chip fixierenden Position. Deutlich ist die Ausnehmung 15 in der Auflagefläche 5 zu erkennen, die im MALDI-Chip fixierenden Zustand von hinten von dem Hebel 14 abgedeckt wird. Die untere Darstellung zeigt den Halter 1 von unten. Der Hebel 14 liegt an dem Halter an und verdeckt die Ausnehmung 15. In dieser Position verhindert der Hebel zum einen, dass ein Gegenstand durch die Ausnehmung 15 gesteckt wird, die den MALDI-Chip aus seiner Fixierung drückt. Des weiteren wird von dem Hebel 14 der Schieber 4 blockiert, so dass dieser nicht in ein rückgezogene Stellung überführbar ist. FIG. 2a shows the holder 1 according to the invention in the MALDI chip fixing state, the MALDI chip has been omitted for illustrative purposes. The holder 1 has a first and a second contact surface 2, 3, on which the MALDI chip 6 (not shown in this figure) is applied. Between the contact surfaces 2, 3, a recess 10 is arranged, which is required for a processing of the contact surfaces 2, 3, but also prevents the MALDI chip is applied only at its corner. Furthermore, the holder according to the invention has a support surface 5, on which the MALDI chip 6 rests. By means of the slide 4, which is movable according to the directions indicated by the double arrow 9 and which is biased by a spring (not shown) in the direction of the contact surfaces 2.3, the MALDI chip is pressed against the contact surfaces 2.3. By investing the MALDI chip on the contact surfaces 2.3 its position in the paper plane is clearly determined. The upper illustration shows the slider from above ie from the side where the MALDI chip rests. The slider 4 is located in its front, ie in a position fixing the MALDI chip. Clearly, the recess 15 can be seen in the support surface 5, which is covered in the MALDI chip fixing state from the rear of the lever 14. The bottom view shows the holder 1 from below. The lever 14 abuts against the holder and conceals the recess 15. In this position, the lever prevents on the one hand, that an object is inserted through the recess 15, which presses the MALDI chip from its fixation. Furthermore, the slider 4 is blocked by the lever 14 so that it can not be transferred to a retracted position.

Figur 2b zeigt den Halter gemäß Figur 2a in der hinteren entriegelnden Stellung des Schiebers, in der der MALDI-Chip entnommen bzw. ein neuer MALDI-Chip in den Halter eingelegt werden kann. Der Hebel 14 ist in dieser Stellung im wesentlichen senkrecht zum Halter angeordnet und gibt dadurch die Öffnung 15 frei. Des weiteren wird die Verriegelung des Schiebers 4 durch den Hebel aufgehoben. FIG. 2b shows the holder according to FIG. 2a in the rear unlocking position of the slide, in which the MALDI chip can be removed or a new MALDI chip can be inserted into the holder. The lever 14 is arranged in this position substantially perpendicular to the holder and thereby releases the opening 15. Furthermore, the locking of the slider 4 is released by the lever.

Figur 3 zeigt den MALDI-Chip 6 in seiner exakt fixierten Position. In dieser Darstellung ist erkennbar, dass die Anlageflächen 2,3 angefast sind, so dass die Federkraft des Fixierungsmittels 4 an den Anlageflächen 2,3 auch eine abwärts gerichtete, durch den Pfeil 11 symbolisierte Bewegung induziert, mit der der MALDI-Chip 6 permanent in Anlage mit der Auflagefläche 5 gehalten wird, wodurch sichergestellt ist, dass der MALDI-Chip 6 auch räumlich eindeutig auf dem Halter positioniert ist. FIG. 3 shows the MALDI chip 6 in its exactly fixed position. In this illustration, it can be seen that the abutment surfaces 2, 3 are chamfered, so that the Spring force of the fixing means 4 to the contact surfaces 2.3 also induces a downward, symbolized by the arrow 11 movement, with which the MALDI chip 6 is permanently held in contact with the support surface 5, thereby ensuring that the MALDI chip. 6 also spatially clearly positioned on the holder.

Figur 4 zeigt den Schieber des Erfindungsgemäßen Halters, der eine Anlagefläche 7 aufweist, die der Eckkontur 8 des MALDI-Chips 6 entspricht. Auch die Anlagefläche 7 ist angefast, so dass der MALDI-Chip auch von dieser Fläche gegen die Auflagefläche gedrückt wird. Der Hebel 14, der hier in der verriegelnden Stellung dargestellt ist, berührt den Schieber 4 in dieser Stellung nicht. Der Schieber 4 hat in dieser Stellung Kontakt zu der Eckkontur 8 des MALDI-Chips 6 und drückt diesen an die Anlageflächen 2,3. FIG. 4 shows the slider of the inventive holder, which has a contact surface 7, which corresponds to the corner contour 8 of the MALDI chip 6. Also, the contact surface 7 is chamfered, so that the MALDI chip is also pressed by this surface against the support surface. The lever 14, which is shown here in the locking position, does not touch the slider 4 in this position. The slider 4 has contact in this position with the corner contour 8 of the MALDI chip 6 and presses it against the contact surfaces 2, 3.

Figur 5 zeigt wie ein MALDI-Chip in den Erfindungsgemäßen Halter eingelegt wird. Dafür wird zunächst einmal der Schieber 4 nach rechts unten bewegt und in dieser Stellung arretiert. Danach wird der MALDI-Chip so in den Halter eingelegt, dass die abgeflachte Eckkontur 8 in Richtung des Schiebers zeigt. Sobald der MALDI-Chip 6 in dem Halter liegt, wird der Schieber umgelegt und drückt aufgrund der Kraft der vorhandenen Feder (nicht dargestellt) den MALDI-Chip so in die obere linke Ecke, dass er an den Anlageflächen 2, 3 anliegt. FIG. 5 shows how a MALDI chip is inserted into the holder according to the invention. For this, the slider 4 is first moved to the bottom right and locked in this position. Thereafter, the MALDI chip is inserted into the holder so that the flattened corner contour 8 points in the direction of the slider. Once the MALDI chip 6 is in the holder, the slider is folded over and presses due to the force of the existing spring (not shown) the MALDI chip in the upper left corner that it rests against the contact surfaces 2, 3.

Figur 6 zeigt die Unterseite des Halters, die Blattfedern 13 aufweist, die den Halter gegen das Analysengerät vorspannen und einen guten elektrischen Kontakt zum Analysengerät sicherstellen. FIG. 6 shows the underside of the holder, the leaf springs 13 which bias the holder against the analyzer and ensure good electrical contact with the analyzer.

Bezugszeichenreference numeral

11 Halterholder 2,32.3 Anlageflächencontact surfaces 44 Fixierungsmittelfixative 55 Auflageflächebearing surface 66 MALDI-ChipMALDI chip 77 Anlageflächecontact surface 88th Eckkonturcorner contour 99 Doppelpfeil, Bewegung des SchiebersDouble arrow, movement of the slider 1010 Ausnehmungrecess 1111 Pfeil, Richtung der Andrückkraft in Richtung der AuflageflächeArrow, direction of the pressure force in the direction of the support surface 1212 Punkte, auf die die MALDI-Matrix dosierbar sindPoints to which the MALDI matrix can be dosed 1313 Federmittel, BlattfederSpring means, leaf spring 1414 Hebel (in geschlossener und in offener Stellung einrastbarLever (can be latched in closed and in open position 1515 Ausnehmung, BohrungRecess, bore 1616 Leiterbahnconductor path 1717 vergoldeter Randbereichgilded border area

Claims (10)

  1. A holder (1) for a MALDI chip (6), having a broken corner contour (8), comprising a first and a second respectively chamfered abutting surface (2, 3) and a movable fixing means (4), which interacts with the broken corner contour (8) and thereby prestresses the MALDI chip (6) against the abutting surfaces (2, 3), wherein the holder has a bearing surface (5), characterized in that the abutting surfaces (2, 3) are chamfered in such a way that the MALDI chip (6) is prestressed against the bearing surface (5).
  2. The holder as claimed in claim 1, characterized in that the fixing means (4) has an abutting surface (7), the contour of which is complementary to the corner contour (8) of the MALDI chip (6).
  3. The holder as claimed in claim 2, characterized in that the abutting surfaces (7) are chamfered in such a way that the MALDI chip bears permanently on the bearing surface (5).
  4. The holder as claimed in one of the preceding claims, characterized in that the fixing means is a slider (4).
  5. The holder as claimed in claim 4, characterized in that the slider (4) has a spring that prestresses it in the direction of the abutting surfaces (2, 3).
  6. The holder as claimed in one of the preceding claims, characterized in that the abutting surfaces are at least partly coated with a material having a very good electrical conductivity, preferably gold.
  7. The holder as claimed in one of the preceding claims, characterized in that it has a bore (15) in the bearing surface (5).
  8. The holder as claimed in one of the preceding claims, characterized in that spring means (13) are arranged on the opposite side (16) of the holder to the bearing surface (5).
  9. The holder as claimed in one of the preceding claims, characterized in that it has a lever (14) that is reversibly transferrable from a locking to an unlocking position.
  10. A system comprising a holder as claimed in one of claims 1 to 9 and a MALDI chip.
EP06707939.2A 2005-02-01 2006-01-31 Maldi chip holder Not-in-force EP1846165B1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE200520001641 DE202005001641U1 (en) 2005-02-01 2005-02-01 Holder for matrix-assisted laser desorption and ionization analysis chip, includes resting surfaces and moveable fastener
PCT/EP2006/050573 WO2006082193A2 (en) 2005-02-01 2006-01-31 Maldi chip holder

Publications (2)

Publication Number Publication Date
EP1846165A2 EP1846165A2 (en) 2007-10-24
EP1846165B1 true EP1846165B1 (en) 2013-05-22

Family

ID=34707023

Family Applications (1)

Application Number Title Priority Date Filing Date
EP06707939.2A Not-in-force EP1846165B1 (en) 2005-02-01 2006-01-31 Maldi chip holder

Country Status (4)

Country Link
US (1) US20090212233A1 (en)
EP (1) EP1846165B1 (en)
DE (1) DE202005001641U1 (en)
WO (1) WO2006082193A2 (en)

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4159875A (en) * 1976-10-21 1979-07-03 Abbott Laboratories Specimen holder
US5592289A (en) * 1995-01-09 1997-01-07 Molecular Dynamics Self-aligning mechanism for positioning analyte receptacles
ATE334397T1 (en) * 1999-12-21 2006-08-15 Tecan Trading Ag CLAMPING DEVICE FOR RECEIVING AND PRECISELY POSITIONING AN OBJECT, PREFERABLY A MICROTITER PLATE, AND METHOD FOR OPERATING IT
AU2001271317B2 (en) * 2000-06-15 2006-08-10 Irm, Llc Automated precision object holder
KR20040105360A (en) * 2003-06-07 2004-12-16 삼성전자주식회사 Sample holder for laser desorption/inoization mass spectrometry and method of manufacturing thereof
US6825478B1 (en) * 2003-10-10 2004-11-30 Perseptive Biosystems, Inc. MALDI plate with removable magnetic insert

Also Published As

Publication number Publication date
WO2006082193A2 (en) 2006-08-10
EP1846165A2 (en) 2007-10-24
US20090212233A1 (en) 2009-08-27
DE202005001641U1 (en) 2005-06-23
WO2006082193A3 (en) 2007-03-22

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