EP1833514A2 - Phospholipid ether analogs for detecting and treating cancer - Google Patents

Phospholipid ether analogs for detecting and treating cancer

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Publication number
EP1833514A2
EP1833514A2 EP05858499A EP05858499A EP1833514A2 EP 1833514 A2 EP1833514 A2 EP 1833514A2 EP 05858499 A EP05858499 A EP 05858499A EP 05858499 A EP05858499 A EP 05858499A EP 1833514 A2 EP1833514 A2 EP 1833514A2
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EP
European Patent Office
Prior art keywords
cancer
tumor
para
group
imaging
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Application number
EP05858499A
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German (de)
French (fr)
Inventor
Jamey Weichert
Marc Longino
Anatoly Pinchuk
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Cellectar Inc
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Cellectar Inc
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Publication of EP1833514A2 publication Critical patent/EP1833514A2/en
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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K51/00—Preparations containing radioactive substances for use in therapy or testing in vivo
    • A61K51/02—Preparations containing radioactive substances for use in therapy or testing in vivo characterised by the carrier, i.e. characterised by the agent or material covalently linked or complexing the radioactive nucleus
    • A61K51/04—Organic compounds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K51/00—Preparations containing radioactive substances for use in therapy or testing in vivo
    • A61K51/02—Preparations containing radioactive substances for use in therapy or testing in vivo characterised by the carrier, i.e. characterised by the agent or material covalently linked or complexing the radioactive nucleus
    • A61K51/04—Organic compounds
    • A61K51/0404—Lipids, e.g. triglycerides; Polycationic carriers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K51/00—Preparations containing radioactive substances for use in therapy or testing in vivo
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A61P35/04—Antineoplastic agents specific for metastasis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer

Definitions

  • the invention generally relates to phospholipids ether analogs and use thereof and specifically relates to use of phospholipid ether analogs and combinations thereof for diagnosis of metastasis, treatment, pharmacokinetic, dosimetry, and toxicity studies of various cancer types, such as non-small cell lung cancer, prostate cancer and metastasis thereof.
  • NSCLC Non-small cell lung cancer
  • NSCLC Non-small cell lung cancer
  • Imaging with FDG PET scanning has recently become the "gold standard" for imaging NSCLC, due to improved sensitivity, particularly when compared with CT imaging.
  • its sensitivity for identifying mediastinal lymph node involvement is only about 90%, and lack of specificity, particularly in patients with inflammatory or granulomatous disease, is particularly problematic.
  • its utility in diagnosing brain tumors or metastases is limited due to high metabolic background of normal brain tissue.
  • CT computed tomography
  • MRI magnetic resonance imaging
  • PET Positron-emission tomography
  • FDG F- fluorodeoxyglucose
  • FDG-PET has been shown to reduce futile thoracotomies in patients. However, because of the false positive and false negative rate, confirmatory mediastinoscopies are often recommended. For example, a retrospective study involving over 200 patients with NSCLC found that the sensitivity, specificity, positive and negative predictive values, and accuracy for FDG-PET were 64%, 77%, 45%, 88%, and 75%, respectively.
  • FDG-PET also plays a role in diagnosing extra-thoracic disease, particularly in patients with intermediate stages of lung cancer. A study done by the American College of Physicians involving over 300 patients found that unsuspected metastatic disease or second primary malignancies was identified in 1 8 of 287 patients (6.3%).
  • the sensitivity and specificity for the identification of cerebral metastatic disease in patients with NSCLC was 60% and 99% for FDG-PET, and 100% and 100% for conventional imaging. Therefore, FDG-PET imaging is not considered to be the best method of evaluating a patient with NSCLC for metastatic disease to the brain.
  • FDG fluorescence desorption spectroscopy
  • Another disadvantage of FDG is that it is not specific for tumors, but accumulates in both malignant and non-malignant hypermetabolic tissues.
  • FDG is a nonspecific tracer and accumulates in areas of infection or inflammation. In the lung, these areas can be localized lung parenchymal nodules or more diffuse (subsegmental, segmental or lobar) or in the hilar and mediastinal nodes.
  • FDG-PET is also frequently negative in malignancies with a low metabolic rate, such as bronchoalveolar carcinoma or carcinoid.
  • a radiopharmaceutical that could accurately identify early metastatic disease in the patients with NSCLC would have a significant impact on patient care, in terms of both staging and response to therapy.
  • PET imaging has improved diagnostic efficacy in this area compared to CT, there remains a need for an accurate imaging technique that is not based upon metabolic activity, which is non-specific, but is based upon a tumor-specific function that can non-invasively screen the whole body, including the brain, TPara 181 Prostate Cancer
  • Factors increasing the likelihood of systemic recurrence include a high preoperative PSA level as well as pathologic features of the surgical specimen including Cleason score >7, seminal vesicle involvement, and lymph node involvement. In contrast, extracapsular extension, positive surgical margins and Gleason score ⁇ 7 are factors generally associated with local recurrence.
  • the velocity of PSA rise following prostatectomy has been utilized to determine whether disease recurrence is local or systemic. For instance, Partin et al reported that a PSA rise of less than 0.75 ng/ml/year was more frequently associated with local recurrence.
  • patients with a rising PSA due to local recurrence in whom systemic recurrence cannot be excluded with confidence, may unnecessarily undergo hormonal ablation, which is generally not considered curative and is associated with osteoporosis development, decreased libido, weight gain, menopausal symptoms, and overall malaise, as well as the evolution of hormonally independent prostate cancer.
  • CT computed tomography
  • MRI magnetic resonance imaging
  • Radioimmunoscintigraphy with lndium-1 1 1 capromab pendetide has been utilized in patients following prostatectomy with a rising PSA who have a high clinical suspicion of occult metastatic disease and no clear evidence for metastatic disease in other imaging studies.
  • This scan is based on a radiolabeled murine monoclonal antibody which is specific for PSMA (Prostate-specific membrane antigen), a transmembrane protein which is specifically expressed by both normal and malignant prostate epithelial cells.
  • PSMA Prostate-specific membrane antigen
  • ProstaScint radioimmunoscintigraphy has been shown to be promising in diagnosing locally recurrent disease in the prostate bed in patients with rising PSA
  • clinical results for this scan have been somewhat variable, with sensitivities ranging between 44% and 92% and specificities between 36% and 86%.
  • false-negative ProstaScint studies have been reported in 10% to 20% of cases.
  • ProstaScint has been reported in neurofibromatosis, lymphomas, renal carcinomas, pelvic kidneys, myolipomas, and meningiomas, as well as in the bone marrow of vertebral bodies. Given this data, use of the ProstaScint scan for patients at risk for occult metastases from prostate cancer remains controversial.
  • PET positron-emission tomography
  • FDC-PET can distinguish between active and quiescent bone metastases in patients with prostate cancer.
  • the intensity of FDG uptake is thought to reflect the metabolic and biological activity of these lesions in contrast to the traditional bone scan with technetium-diphosphonate compounds in which nonspecific osteoblast activity may be detected as a false positive signal following treatment.
  • a false negative reading may be obtained since early metastases, which initially seed into the bone marrow, will not necessarily produce a signal until an osteoblastic response occurs. Therefore, a persistently positive bone scan does not necessarily indicate the presence of residual viable metastases and a negative bone scan result may not reflect accurately the patient's metastatic tumor burden.
  • FDC-PET may therefore prove beneficial in guiding the management of patients with bony metastases and in a retrospective study FDC-PET and helical CT have been shown independently to be more effective than i n In-monoclonal antibody imaging in detecting metastatic disease.
  • the present invention provides a method for detecting and locating recurrence of cancer, radiation and chemo insensitive cancer or metastasis of cancer selected from the group consisting of Lung cancer, Adrenal cancer, Melanoma, Colon cancer, Colorectal cancer, Ovarian cancer, Prostate cancer, Liver cancer, Subcutaneous cancer, Squamous cell cancer, Intestinal cancer, Hepatocellular carcinoma, Retinoblastoma, Cervical cancer, Glioma, Breast cancer and Pancreatic cancer in subject that has or is suspected of having cancer.
  • the method comprising the steps of: (a) administering a phospholipid ether analog to the subject; and (b) determining whether an organ suspected of having recurrence of cancer, radiation and chemo insensitive cancer or metastasis of cancer in the subject retains a higher level of the analog than surrounding region(s) wherein a higher retention region indicates detection and location of the recurrence of cancer, radiation insensitive and chemo cancer or metastasis of cancer.
  • the phospholipid analog is selected from:
  • X is selected from the group consisting of radioactive isotopes of halogen; n is an integer between 8 and 30; and Y is selected from the group comprising NH2, NR2, and NR3, wherein R is an alkyl or arylalkyl substituent or
  • X is a radioactive isotope of halogen
  • n is an integer between 8 and 30
  • Y is selected from the group consisting of H, OH 1 COOH, COOR and OR
  • 2 is selected from the group consisting of NH2, NR2, and NR3, wherein R is an alkyl or arylalkyl substituent.
  • X is selected from the group of radioactive halogen isotopes consisting of 1 SF 1 ⁇ a, 76Br, 77 ⁇ r , 82 B r,i22
  • the phospholipid ether isl 8-(p-lodophenyl)octadecyl phospnocboltne, 1 -O-[l 8-(p-lodophenyl)octadecyl]-l ,3-propanediol-3- phosphocholine, or 1 -O-D ⁇ - ⁇ -lodophenyOoctadecylJ-ii.-O-methyl-rac- glycero-3-phosphocholine, wherein iodine is in the form of a radioactive isotope.
  • the detection is carried out by a of PET, CT, MRI scanning methods combination thereof.
  • Another embodiment of the present invention provides a method for the treatment of recurrence of cancer, radiation and chemo insensitive cancer or metastasis of cancer in a subject.
  • the method comprises administering to the subject an effective amount of a compound comprising a phospholipid ether analog, in a preferred embodiment, the recurrence of cancer, radiation and chemo insensitive cancer or metastasis of cancer occurs in the group selected from Lung cancer, Adrenal cancer, Melanoma, Colon cancer, Colorectal cancer, Ovarian cancer, Prostate cancer, Liver cancer, Subcutaneous cancer, Squamous cell cancer, Intestinal cancer, Hepatocellular carcinoma, Retinoblastoma, Cervical cancer, Glioma, Breast cancer, Pancreatic cancer and Carcinosarcoma.
  • the phospholipid analog is selected from:
  • X is selected from the group consisting of radioactive isotopes of halogen; n is an integer between 8 and 30; and Y is selected from the group comprising NHh 1 NR2, and NR3, wherein R is an alkyl or arylalkyl substituent or
  • X is a radioactive isotope of halogen
  • n is an integer between 8 and 30
  • Y is selected from the group consisting of H, OH, COOH, COOR and OR
  • Z is selected from the group consisting of NH2, NR 2 , and NR3, wherein R is an alkyl or arylalkyl substituent.
  • X is selected from the group of radioactive halogen isotopes consisting of i8F, 36Cl, 76Br, 77Br, 8 2 ⁇ r,i22
  • the effective amount of phospholipid ether analog is a combination of at least two isotopes, one with one with a path range of about 0.1 A to 1 mm and a second with a path range of about 1 mm to 1 m.
  • the effective amount of phospholipid ether analog is a combination of at least two isotopes, 125 I and 131 I.
  • the phospholipid ether isl 8-(p-lodophenyl)octadecyl phosphocholine, 1 -O-[l 8-(p-lodophenyl)octadecyl]-l ,3-propanediol-3- phosphocholine, or l -O-[l 8-(p-lodophenyl)octadecyl]-2 ⁇ O-methyl-rac- glycero-3-phosphocholine, wherein iodine is in the form of a radioactive isotope.
  • the effective amount of phospholipid ether analog is fractionated. In yet other embodiments, the effective amount of phospholipid ether analog is about O.S ⁇ Ci to about 3Ci treatable in a linear and dose dependent manner. Other embodiments provide that the dosage is adaptable to the cancer-volume. Yet other embodiments provide that the dosage for radiation insensitive tumor is greater than dosage for radiation sensitive tumor and less than 3Ci and is adaptable to the cancer- volume.
  • Another embodiment of the present invention provides the use of phospholipid ether analog for the production of a pharmaceutical composition for the treatment of recurrence of cancer, radiation and chemo insensitive cancer or metastasis of cancer.
  • the phospholipid analog is selected from:
  • X is selected from the group consisting of radioactive isotopes of halogen; n is an integer between 8 and 30; and Y is selected from the group comprising NHz, NR2, and NR3, wherein R is an alkyl or arylalkyl substituent or
  • X is a radioactive isotope of halogen
  • n is an integer between 8 and 30
  • Y is selected from the group consisting of H, OH, COOH, COOR and OR
  • Z is selected from the group consisting of NH2, NR2, and NR3, wherein R is an alkyl or arylalkyl substituent.
  • X is selected from the group of radioactive halogen isotopes consisting of i»F f 36 CI, 76 Br, 77 Br, sz ⁇ r ⁇ l, '23
  • the phospholipid ether isl 8-(p- lodophenyOoctadecyl phosphocholine, 1 -O-D 8-(p-lodophenyl)octadecyl]- 1 ,3-propanediol-3-phosphocholine, or 1 -O-[l 8-(p-lodophenyl)octadecyl]- 2-0-methyl-rac-glycero-3-phosphocholine, wherein iodine is in the form of a radioactive isotope.
  • Elastically scattered electrons (which include backscattered electrons) are generally scattered through larger angles than are inelastically scattered electrons, (right)
  • An incident electron ionizes the sample atom by ejecting an electron from an inner-shell (the K shell, in this case).
  • De- excitation in turn, produces characteristic X-radiation (above) or an Auger electron (below).
  • Secondary electrons are ejected with low energy from outer loosely bound electron shells, a process not shown.
  • Fig. 3 Scintigraphy of the anterior chest of Patient 03 acquired at 1 , 2, and 6 days after iv administration of 1 mCi 1 31 l-NM-324. Uptake is seen in the left lingular lung cancer (T) with increasing tumor-to-background ratios over time.
  • Fig. 4 Time course (days) of NM404 in a SCID mouse with a human RL-251 adrenal tumor (T) xenograft.
  • FIG. 5 Tissue distribution 1-1 125-NM404 in RL 251 Adrenal Cancer in SCID mice depicting that while accumulation in the tumor increased, distribution in blood, spleen and kidney reduced by days 1 through 14.
  • Fig. 6 Apparent SCCl and SCC6 tumor regression after injection of 125I-NM404. By day 41 , the tumor is significantly reduced.
  • Fig. 7. The image shows one of the tumor-bearing animals treated with 250 ⁇ Ci of I-125-NM404 at 4 weeks following injection. The hair above the tumor has fallen off, presumably due to the strong accumulation of radioactivity in the tumor. Additionally, the surface of the tumors appears "caved in” and shows darker areas, presumably from hemorrhage and necrosis. The figure shows the effect of 1-125-NM404 on the tumor. Although tumor size (outer dimensions) may not shrink, 1-125- NM404 causes central necrosis. The measurement method measuring outer tumor dimensions may have underestimated tumor volume response following I-125-NM404.
  • FIG. 8 PC3 human prostate cancer model implanted into SCID mouse.
  • PC3 is known to be radiation insensitive.
  • the curves between control (no radioactivity, cold NM404) and treatment (1-1 25-NM404) only separate about 4-5 weeks after treatment, until then the growth of the tumors looks the same indicating that: 1) NM404 takes a few days to about 1 week to fully accumulate in the tumor, and 2) the isotope 1-125 has a low radiation flow (since it has a long half life). Both factors contribute to a delayed onset of the therapy effect, at a time when all NM404 has cleared out of normal tissues.
  • Fig. 9 Scintigraphic comparison of NM404 (bottom panel) and NM324 (top panel) at 1 , 2, and 4 days in a SCID mouse with human prostate PC-3 tumor (arrow) implanted in the flank. Liver and background radioactivity are much improved with NM404.
  • Fig. 10 Tumor volume for each group was recorded over the 10-week assessment period depicting the control and dosage of 50, 1 50, 250 and 500 ⁇ Ci.
  • control animals show rapidly growing tumors over the 10-week assessment period.
  • the 50 uCi dose group did not show any difference to control animals, hence these seem to be ineffective dose levels in this animal model.
  • the 1 50, 250 and 500 ⁇ Ci dose groups show a substantial and prolonged treatment effect. Tumor volumes are stable and same tumors appear "collapsed" (the tumor surface has caved in). Additionally, hair above the tumors fell off confirming substantial accumulation of radioactivity in these tumors.
  • Fig. 1 A549 Tumor Xenografts (I Xl O 6 cells, s.c.) in Female SCID Mice Fractionated Dose (3 x 50 mCi), having 2 independent controls for each dosage.
  • a fractionated dosing of NM404 e.g. 3 x 50 micro-Ci versus a single dose of 150 micro-Ci
  • the fractionated dose may be safer since it is eliminated from normal tissues in between fractionated injections.
  • FIG. 12 A549 Large Tumors vs Small Tumors 1 50 microcuries
  • FIG. 13 Bioscan image (A) obtained 4 days post I Z5
  • FIG. 1 Fused 3D surface-rendered MRI image (blue) and 3D , microPET image (A) obtained 24h after iv injection of 124 I-NM4O4 (80 ⁇ Ci) into a rat with a CNS-I glioma brain tumor. Images were fused using Amira
  • 124 l-MicroPET images a human A549 lung tumor xenograft in a SCID mouse 48h post injection of 124 I-NM4O4 (80 ⁇ Ci).
  • Fig. 1 7. i 2 ⁇ -MicroPET "mages (coronal, sagittal, and axial) 96h after iv injection of 124 I-NM4O4 into a PC-3 (flank) tumor -bearing mouse.
  • NM324 top panel at 1 , 2, and 4 days in a SCID mouse with human prostate
  • PC-3 tumor implanted in the flank. Liver and background radioactivity are much improved with NM404.
  • Fig. 20 Time course of 125 I-NM4O4 in human RL-251 adrenal cancer xenograft in a SCID mouse. Prolonged tumor (1 .5x0.5 cm, arrow) retention is evident even after 20 days.
  • Bioscan image obtained 4 days post NM404 injection (B). Positionally matched fused photo/Bioscan image of excised prostate/vesicular gland (C) showing intense uptake of radioactivity in the prostate tumor.
  • Nude mouse leg at various times following intratibial injection (arrow in first panel depicts injection site and direction) of 2x10 5 human PC3 prostate tumor cells. Tumor begins protruding through the bone by 28 days (arrow) and by 46 days the tumor has literally destroyed the tibia leaving only the fibula intact.
  • FIG. 24 Fused 3D surface-rendered MRI image (blue) and 3D microPET image (A) obtained 24h after iv injection of 124 I-NM4O4 (100 ⁇ Ci) into a rat with a CNS-I glioma brain tumor. Images were fused using Amira (v3.1 ). Right panels show (B) contrast-enhanced coronal MRI slice through the tumor (arrow) and (C) fused coronal MRI and 124 I-NM4O4 microPET images corroborating presence and location of the tumor.
  • FIG. 25 Posterior whole body planar nuclear medicine image (A) 4 days after iv administration of 13 'l-NM-404 (0.8 mCi) to a patient with non small cell lung cancer (6 cm dia., arrow). Lung tumor is easily detected in corresponding axial (B) and coronal (C) computed tomography (CT) scans.
  • the term “isomer” includes, but is not limited to optical isomers and analogs, structural isomers and analogs, conformational isomers and analogs, and the like.
  • this invention encompasses the use of different optical isomers of an anti-tumor compound of Formula 3A.
  • the anti-tumor compounds useful in the present invention may contain at least one chiral center. Accordingly, the compounds used in the methods of the present invention may exist in, and be isolated in, optically-active or racemic forms. Some compounds may also exhibit polymorphism.
  • the present invention may encompass the use of any racemic, optically-active, polymorphic, or stereroisomeric form, or mixtures thereof, which form possesses properties useful in the treatment of tumor-related conditions described and claimed herein.
  • the anti-tumor compounds may include pure (R)-isomers.
  • the anti-tumor compounds may include pure (S)- isomers.
  • the compounds may include a mixture of the (R) and the (S) isomers.
  • the compounds may include a racemic mixture comprising both (R) and (S) isomers.
  • optically-active forms for example, by resolution of the racemic form by recrystallization techniques, by synthesis from optically-active starting materials, by chiral synthesis, or by chromatographic separation using a chiral stationary phase.
  • the invention includes the use of pharmaceutically acceptable salts of amino-substituted compounds with organic and inorganic acids, for example, citric acid and hydrochloric acid.
  • the invention also includes N- oxides of the amino substituents of the compounds described herein.
  • Pharmaceutically acceptable salts can also he prepared from the phenolic compounds by treatment with inorganic bases, for example, sodium hydroxide.
  • esters of the phenolic compounds can be made with aliphatic and aromatic carboxylic acids, for example, acetic acid and benzoic acid esters.
  • pharmaceutically acceptable salt refers to a compound formulated from a base compound which achieves substantially the same pharmaceutical effect as the base compound.
  • This invention further includes method utilizing derivatives of the anti-tumor compounds.
  • derivatives includes but is not limited to ether derivatives, acid derivatives, amide derivatives, ester derivatives and the like.
  • this invention further includes methods utilizing hydrates of the anti-tumor compounds.
  • hydrate includes but is not limited to hemihydrate, monohydrate, dihydrate, trihydrate and the like.
  • This invention further includes methods of utilizing metabolites of the anti-tumor compounds.
  • the term "metabolite” means any substance produced from another substance by metabolism or a metabolic process.
  • "contacting" means that the anti-tumor compound used in the present invention is introduced into a sample containing the receptor in a test tube, flask, tissue culture, chip, array, plate, microplate, capillary, or the like, and incubated at a temperature and time sufficient to permit binding of the anti-tumor compound to a receptor. Methods for contacting the samples with the anti-tumor compound or other specific binding components are known to those skilled in the art and may be selected depending on the type of assay protocol to be run.
  • the term "contacting" means that the anti-tumor compound used in the present invention is introduced into a patient receiving treatment, and the compound is allowed to come in contact in vivo.
  • the term “treating” includes preventative as well as disorder remittent treatment.
  • the terms “reducing”, “suppressing” and “inhibiting” have their commonly understood meaning of lessening or decreasing.
  • progression means increasing in scope or severity, advancing, growing or becoming worse.
  • recurrence means the return of a disease after a remission.
  • administering refers to bringing a patient, tissue, organ or cells in contact with an anti-tumor phospholipid ether compound.
  • administration can be accomplished in vitro, i.e. in a test tube, or in vivo, i.e. in cells or tissues of living organisms, for example, humans.
  • the present invention encompasses administering the compounds useful in the present invention to a patient or subject.
  • a “patient” or “subject”, used equivalently herein, refers to a mammal, preferably a human, that either: (1 ) has a disorder remediable or treatable by administration of the anti-tumor substance using a phospholipid ether compound or (2) is susceptible to a disorder that is preventable by administering the anti-tumor compound using a phospholipid ether compound
  • pharmaceutical composition means therapeutically effective amounts of the anti-tumor compound having radioactivity together with suitable diluents, preservatives, solubilizers, emulsifiers, and adjuvants, collectively “pharmaceutically-acceptable carriers.”
  • effective amount and “therapeutically effective amount” refer to the quantity of active therapeutic agent sufficient to yield a desired therapeutic response without undue adverse side effects such as toxicity, irritation, or allergic response.
  • the specific "effective amount” will, obviously, vary with such factors as the particular condition being treated, the physical condition of the patient, the type of animal being treated, the duration of the treatment, the nature of concurrent therapy (if any), and the specific formulations employed and the structure of the compounds or its derivatives. In this case, an amount would be deemed therapeutically effective if it resulted in one or more of the following: (a) the prevention of disease (e.g., pancreatic cancer, breast cancer); and (b) the reversal or stabilization of such disease.
  • the optimum effective amounts can be readily determined by one of ordinary skill in the art using routine experimentation.
  • compositions are liquids or lyophilized or otherwise dried formulations and include diluents of various buffer content (e.g., Tris-HCI, acetate, phosphate), pH and ionic strength, additives such as albumin or gelatin to prevent absorption to surfaces, detergents (e.g., Tween (Polysorbate) 20, Tween 80, Pluronic F68, bile acid salts), solubilizing agents (e.g., glycerol, polyethylene glycerol), anti-oxidants (e.g., ascorbic acid, sodium metabisulfite), preservatives (e.g., Thimerosal, benzyl alcohol, parabens), bulking substances or tonicity modifiers (e.g., lactose, mannitol), covalent attachment of polymers such as polyethylene glycol to the protein, complexation with metal ions, or incorporation of the material into or onto particulate preparations of polymeric compounds such as
  • compositions coated with polymers e.g., poloxamers or poloxamines
  • Other embodiments of the compositions incorporate particulate forms protective coatings, protease inhibitors or permeation enhancers for various routes of administration, including topical, parenteral, pulmonary, nasal and oral.
  • the pharmaceutical composition is administered parenterally, paracancerally, transmucosally, tansdermally, intramuscularly, intravenously, intradermally, subcutaneously, intraperitonealy, intraventricular ⁇ , intracraniaJly and intratumorally.
  • pharmaceutically acceptable carriers are well known to those skilled in the art and include, but are not limited to, 0,0T-0.1 M and preferably 0.05M phosphate buffer or 0.9% saline. Additionally, such pharmaceutically acceptable carriers may be aqueous or non-aqueous solutions, suspensions, and emulsions. Examples of nonaqueous solvents are propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable organic esters such as ethyl oleate. Aqueous carriers include water, alcoholic/aqueous solutions, emulsions or suspensions, including saline and buffered media.
  • Parenteral vehicles include sodium chloride solution, Ringer's dextrose, dextrose and sodium chloride, lactated Ringer's and fixed oils.
  • Intravenous vehicles include fluid and nutrient replenishers, electrolyte replenishers such as those based on Ringer's dextrose, and the like. Preservatives and other additives may also be present, such as, for example, antimicrobials, antioxidants, collating agents, inert gases and the like.
  • Controlled or sustained release compositions administerable according to the invention include formulation in lipophilic depots (e.g. fatty acids, waxes, oils). Also comprehended by the invention are particulate compositions coated with polymers (e.g. poloxamers or poloxamines) and the compound coupled to antibodies directed against tissue-specific receptors, ligands or antigens or coupled to ligands of tissue-specific receptors.
  • compositions administered according to the invention incorporate particulate forms, protective coatings, protease inhibitors or permeation enhancers for various routes of administration, including parenteral, pulmonary, nasal and oral.
  • a pharmaceutical composition can be delivered in a controlled release system.
  • the agent may be administered using intravenous infusion, an implantable osmotic pump, a transdermal patch, liposomes, or other modes of administration.
  • a pump may be used (see Langer, supra; Sefton, CRC Crit. Ref. Biomed. Eng. 14:201 (1987); Buchwald et al., Surgery 88:507 (1980); Saudek et al., N. Engl. J. Med.
  • a controlled release system can be placed in proximity to the therapeutic target, for example liver, thus requiring only a fraction of the systemic dose (see, e.g., Goodson, in Medical Applications of Controlled Release, supra, vol. 2, pp. 1 1 5-1 38 (1984). Other controlled release systems are discussed in the review by Langer (Science 249:1 527-1 533 (1990).
  • the pharmaceutical preparation can comprise the anti-tumor compound alone, or can further include a pharmaceutically acceptable carrier, and can be in solid or liquid form such as tablets, powders, capsules, pellets, solutions, suspensions, elixirs, emulsions, gels, creams, or suppositories, including rectal and urethral suppositories.
  • Pharmaceutically acceptable carriers include gums, starches, sugars, cellulosic materials, and mixtures thereof.
  • the pharmaceutical preparation containing the anti-tumor compound can be administered to a patient by, for example, subcutaneous implantation of a pellet.
  • a pellet provides for controlled release of anti-tumor compound over a period of time.
  • the preparation can also be administered by intravenous, intra-arterial, or intramuscular injection of a liquid preparation oral administration of a liquid or solid preparation, or by topical application, Administration can also be accomplished by use of a rectal suppository or a urethral suppository.
  • the pharmaceutical preparations administerable by the invention can be prepared by known dissolving, mixing, granulating, or tablet-forming processes.
  • the anti-tumor compounds or their physiologically tolerated derivatives such as salts, esters, N-oxides, and the like are mixed with additives customary for this purpose, such as vehicles, stabilizers, or inert diluents, and converted by customary methods into suitable forms for administration, such as tablets, coated tablets, hard or soft gelatin capsules, aqueous, alcoholic or oily solutions.
  • suitable inert vehicles are conventional tablet bases such as lactose, sucrose, or cornstarch in combination with binders such as acacia, cornstarch, gelatin, with disintegrating agents such as cornstarch, potato starch, alginic acid, or with a lubricant such as stearic acid or magnesium stearate.
  • suitable oily vehicles or solvents are vegetable or animal oils such as sunflower oil or fish-liver oil. Preparations can be effected both as dry and as wet granules.
  • the anti-tumor compounds or their physiologically tolerated derivatives such as salts, esters, N-oxides, and the like are converted into a solution, suspension, or expulsion, if desired with the substances customary and suitable for this purpose, for example, solubilizers or other auxiliaries.
  • sterile liquids such as water and oils, with or without the addition of a surfactant and other pharmaceutically acceptable adjuvants.
  • Illustrative oils are those of petroleum, animal, vegetable, or synthetic origin, for example, peanut oil, soybean oil, or mineral oil.
  • water, saline, aqueous dextrose and related sugar solutions, and glycols such as propylene glycols or polyethylene glycol are preferred liquid carriers, particularly for injectable solutions.
  • compositions which contain an active component are well understood in the art. Such compositions may be prepared as aerosols delivered to the nasopharynx or as injectables, either as liquid solutions or suspensions; however, solid forms suitable for solution in, or suspension in, liquid prior to injection can also be prepared. The preparation can also be emulsified.
  • the active therapeutic ingredient is , often mixed with excipients which are pharmaceutically acceptable and compatible with the active ingredient. Suitable excipients are, for example, water, saline, dextrose, glycerol, ethanol, or the like or any combination thereof.
  • composition can contain minor amounts of auxiliary substances such as wetting or emulsifying agents, pH buffering agents which enhance the effectiveness of the active ingredient.
  • auxiliary substances such as wetting or emulsifying agents, pH buffering agents which enhance the effectiveness of the active ingredient.
  • An active component can be formulated into the composition as neutralized pharmaceutically acceptable salt forms.
  • Pharmaceutically acceptable salts include the acid addition salts, which are formed with inorganic acids such as, for example, hydrochloric or phosphoric acids, or such organic acids as acetic, oxalic, tartaric, mandelic, and the like.
  • Salts formed from the free carboxyl groups can also be derived from inorganic bases such as, for example, sodium, potassium, ammonium, calcium, or ferric hydroxides, and such organic bases as isopropylamine, trimethylamine, 2-ethylamino ethanol, histidine, procaine, and the like.
  • inorganic bases such as, for example, sodium, potassium, ammonium, calcium, or ferric hydroxides
  • organic bases as isopropylamine, trimethylamine, 2-ethylamino ethanol, histidine, procaine, and the like.
  • the anti-tumor compounds or their physiologically tolerated derivatives such as salts, esters, N-oxides, and the like are prepared and applied as solutions, suspensions, or emulsions in a physiologically acceptable diluent with or without a pharmaceutical carrier.
  • the active compound in another method according to the invention, can be delivered in a vesicle, in particular a liposome (see Langer, Science 249:1 527-1 533 (1 990); Treat et al., in Liposomes in the Therapy of Infectious Disease and Cancer, Lopez-Berestein and Fidler (eds.), Liss, N.Y., pp. 353-365 (1989); Lopez-Berestein ibid., pp. 31 7-327; see generally ibid).
  • the salts of the anti-tumor compound may be pharmaceutically acceptable salts. Other salts may, however, be useful in the preparation of the compounds according to the invention or of their pharmaceutically acceptable salts.
  • Suitable pharmaceutically acceptable salts of the compounds include acid addition salts which may, for example, be formed by mixing a solution of the compound according to the invention with a solution of a pharmaceutically acceptable acid such as hydrochloric acid, sulphuric acid, methanesulphonic acid, fumaric acid, maleic acid, succinic acid, acetic acid, benzoic acid, oxalic acid, citric acid, tartaric acid, carbonic acid or phosphoric acid.
  • a pharmaceutically acceptable acid such as hydrochloric acid, sulphuric acid, methanesulphonic acid, fumaric acid, maleic acid, succinic acid, acetic acid, benzoic acid, oxalic acid, citric acid, tartaric acid, carbonic acid or phosphoric acid.
  • phospholipids ether compounds and specially NM404 is a promising new tumor-selective diagnostic imaging agent to monitor the treatment response of several tumor treatment modalities.
  • Radioiodinated NM404 a second-generation phospholipid ether analog, had displayed remarkable tumor selectivity in 27/27 tumor models. Due to a lack of metabolic phospholipase enzymes in the membranes of tumor cells, the prevailing hypothesis of this approach is that phospholipid ether analogs become trapped exclusively in tumor cell membranes because of their inability to become metabolized and eliminated. Thus, the differential clearance rates of phospholipid ethers from normal cells versus viable tumor cells form the basis of this concept.
  • NM404 is sequestered and selectively retained by viable tumor cells and localizes in both primary and metastatic lesions regardless of anatomic location including those found in lymph nodes. Unlike FDG, this agent does not localize in infectious sites.
  • Other advantages of NM404 over FDG include the following: NM404 is selective for and retained indefinitely by malignant tumor cells whereas FDG in not selective for tumor cells and goes to infectious sites and hyperplasias (Barret's Esophagus). Further, since 124 I has a 4 day physical half life it can be shipped anywhere in the world whereas FDG with its 1 10 min half-life, may have limited distribution within 200 miles of the production site.
  • NM404 undergoes prolonged retention (not metabolized) and therefore affords a significant therapeutic potential when mated with an appropriate radioisotope like 131 I whereas FDG does not possess any therapeutic potential.
  • NM404 can be labeled with a variety of iodine isotopes expanding it versatility (diagnosis and therapy as well as a tool for experimental animal studies) whereas FDG is limited to 18 F for PET scanning or potentially 19 F (stable) for magnetic resonance imaging albeit at very low sensitivity levels. Regardless of its tumor targeting ability, due to its rapid metabolism in tumor cells, it has not potential for therapy.
  • NM404 affords the potential to not only accurately predict local tumor response to various treatment modalities, but also allows detection of distant metastatic lesions in cases of sub-therapeutic primary tumor treatment.
  • the PLE compounds may be designed to more accurately estimate the specificity and sensitivity of radiolabeled PLE analogs such as NM404 as an imaging agent in prostate cancer and other cancers. Based upon preclinical models, PLE analogs such as NM404 are likely to exhibit high uptake in tumors giving this agent the significant potential for use in staging, following response to therapy, or potentially as a therapeutic agent when coupled with higher doses of 131 I, 125 !, or 211 At an alpha-emitting halogen with therapeutic efficacy.
  • the present invention generally provides methods and techniques for the detection and treatment of various cancers.
  • the present invention provides a method for detecting and locating recurrence of cancer, radiation and chemo insensitive cancer or metastasis of cancer selected from the group consisting of Lung cancer, Adrenal cancer, Melanoma, Colon cancer, Colorectal cancer, Ovarian cancer, Prostate cancer, Liver cancer, Subcutaneous cancer, Squamous cell cancer, Intestinal cancer, Hepatocellular carcinoma, Retinoblastoma, Cervical cancer, Glioma, Breast cancer and Pancreatic cancer in subject that has or is suspected of having cancer.
  • the method comprising the steps of: (a) administering a phospholipid ether analog to the subject; and .
  • the phospholipid analog is selected from:
  • X is selected from the group consisting of radioactive halogen isotopes
  • n is an integer between 8 and 30
  • Y is selected from the group comprising NH2, NR2, and NR3, wherein R is an alkyl or arylalkyl substituent or
  • X is a radioactive halogen isotope
  • n is an integer between 8 and 30
  • Y is selected from the group consisting of H, OH 1 COOH, COOR and OR
  • Z is selected from the group consisting of NH2, NR2, and NR3, wherein R is an alkyl or arylalkyl substituent.
  • X is selected from the group of radioactive halogen isotopes consisting of 1 8 F, 36 CI 1 76 Br, 77Br, 82Br,i 22
  • the phospholipid ether isl 8-(p-lodophenyl)octadecyl phosphocholine, 1 -O-[l ⁇ -t ⁇ -lodophenyOoctadecylj-l ,3-propanediol-3- phosphocholine, or 1 -O-[18-(p-lodophenyl)octadecyl]-2-0 ⁇ methyl-rac- glycero-3-phosphocholine, wherein iodine is in the form of a radioactive isotope.
  • the detection is carried out by PET CT MRI scanning methods and combination thereof.
  • alkyl refers to a straight chain, branched or cyclic, saturated or unsaturated aliphatic hydrocarbons.
  • the alkyl group has 1 -16 carbons, and may be unsubstituted or substituted by one or more groups selected from halogen, hydroxy, alkoxy carbonyl, amido, alkylamido, dialkylamido, nitro, amino, alkylamino, dialkylamino, carboxyl, thio and thioalkyl.
  • a "hydroxy” group refers to an OH group.
  • alkoxy refers to an — O-alkyl group wherein alkyl is as defined above.
  • a “thio” group refers to an — SH group.
  • a “thioalkyl” group refers to an -SR group wherein R is alkyl as defined above.
  • An “amino” group refers to an -NH2 group.
  • An “alkylamino” group refers to an — NHR group wherein R is alkyl is as defined above.
  • a “dialkylamino” group refers to an — NRR 1 group wherein R and R 1 are all as defined above.
  • An “amido” group refers to an — CONH2.
  • An “alkylamido” group refers to an — CONHR group wherein R is alkyl is as defined above.
  • a “dialkylamido” group refers to an — CONRR' group wherein R and R 1 are alkyl as defined above.
  • a "nitro” group refers to an NO2 group.
  • a “carboxyl” group refers to a COOH group.
  • aryl includes both carbocyclic and heterocyclic aromatic rings, both monocyclic and fused polycyclic, where the aromatic rings can be 5- or 6-membered rings.
  • Representative monocyclic aryl groups include, but are not limited to, phenyl, furanyl, pyrrolyl, thienyl, pyridinyl, pyrimidinyl, oxazolyl, isoxazolyl, pyrazolyl, imidazolyl, thiazolyl, isothiazolyl and the like.
  • Fused polycyclic aryl groups are those aromatic groups that include a 5- or 6-membered aromatic or heteroaromatic ring as one or more rings in a fused ring system.
  • fused polycyclic aryl groups include naphthalene, anthracene, indolizine, indole, isoindole, benzofuranr benzothiophene, indazole, benzimidazole, benzthiazole, purine, quinoline, isoquinoline, ci ⁇ noline, phthalazine, quinazoline, quinoxaline, 1 ,8- naphthyridine, pteridine, carbazole, acridine, phenazine, phenothiazine, phenoxazine, and azulene.
  • arylalkyl refers to moieties, such as benzyl, wherein an aromatic is linked to an alkyl group which is linked to the indicated position in the PLE compound.
  • the recurrence of cancer, radiation and chemo insensitive cancer or metastasis of cancer occurs in the group selected from Lung cancer, Adrenal cancer, Melanoma, Colon cancer, Colorectal cancer, Ovarian cancer, Prostate cancer, Liver cancer, Subcutaneous cancer, Squamous cell cancer, Intestinal cancer, Hepatocellular carcinoma, Retinoblastoma, Cervical cancer, Glioma, Breast cancer, Pancreatic cancer and Carcinosarcoma.
  • the phospholipid analog is selected from:
  • X is selected from the group consisting of radioactive halogen isotopes halogen; n is an integer between 8 and 30; and Y is selected from the group comprising NHa, NR 2 , and NR3, wherein R is an alkyl or arylalkyl substituent or
  • X is a radioactive halogen isotope
  • n is an integer between 8 and 30
  • Y is selected from the group consisting of H, OH, COOH, COOR and OR
  • Z is selected from the group consisting of NH2, NR2, and NR3, wherein R is an alkyl or arylalkyl substituent.
  • X is selected from the group of radioactive halogen isotopes consisting Of 18 F, 36Cl 1 76Br, 77Br, 82Br 1 ⁇ i 1 i23
  • the effective amount of phospholipid ether analog is a combination of at least two isotopes, one with a path range of about 0.1 ⁇ to 1 mm and a second with a path range of about 1 mm to 1 m, also as discussed in Fig. 2.
  • the effective amount of phospholipid ether analog is a combination of at least two isotopes, 125 I and 131 I.
  • the phospholipid ether is18-(p-lodophenyl)octadecyl phosphocholine, 1 -O-[1 8-(p-lodophenyl)octadecyl] ⁇ l ,3 ⁇ propanediol-3- phosphocholine, or l -O-[l 8-(p-lodophenyl)octadecyl]-2-0-methyl-rac- glycero-3-phosphocholine, wherein iodine is in the form of a radioactive isotope.
  • the effective amount of phospholipid ether analog is fractionated.
  • the advantage of using fractionated dosage is that it allows for the PLE analog to be removed from normal tissues.
  • fractionated dosing of NM404 e.g. 3 x 50 micro-Ci versus a single dose of 150 micro-Ci
  • the effective amount of phospholipid ether analog is about 0.5 ⁇ Ci to about 50OmCi, and as shown in Fig. 10, this is treatable in a linear and dose dependent manner.
  • the dosage is adaptable to the cancer-volume as shown in Fig. 12.
  • the graph in Fig. 12 shows the difference in tumor growth for the same dose of 1-125-NM404 (1 50 microCi) when injecting in animals with small ( ⁇ 1 cm) and large tumors (> l cm).
  • Fig. 1 1 1 The results show that a small tumor showed stunned tumor growth with that dose, however a large tumor population did not show any effects for the same dose, basically behaving like non-radiated control.
  • the graph in Fig. 1 2 further illustrates is that the effective tumor dose should be adjusted to tumor volume and that there is a tumor dose per volume of tumor volume that has to be achieved in order to show efficacy.
  • the dosage for radiation and chemo insensitive tumor is greater than dosage for radiation sensitive tumor and less than 50OmCi and is adaptable to the cancer-volume, as ascertainable by comparing PC3 (Fig. 8) and A549 cancer models (Fig. 10, 1 1 and 1 2), in which PC3 model is radiation insensitive.
  • Another embodiment of the present invention provides the use of phospholipid ether analog for the production of a pharmaceutical composition for the treatment of recurrence of cancer, radiation and chemo insensitive cancer or metastasis of cancer.
  • the phospholipid analog is selected from:
  • X is selected from the group consisting of radioactive halogen isotopes
  • n is an integer between 8 and 30
  • Y is selected from the group comprising NHa, NR2, and NR3, wherein R is an alkyl or arylalkyl substituent or
  • X is a radioactive halogen isotope
  • n is an integer between 8 and 30
  • Y is selected from the group consisting of H 1 OH, COOH, COOR and OR
  • Z is selected from the group consisting of NH2, NR2, and NR3, wherein R is an alkyl or arylalkyl substituent.
  • X is selected from the group of radioactive halogen isotopes
  • the phospholipid ether is18-(p- IodophenyOoctadecyl phosphocholine, 1 -O-ll 8-(p-lodophenyl)octadecyl]- 1 ,3 ⁇ propanediol-3-phosphocholine, or 1 -0-[I 8-(p-lodophenyl)octadecyl]- 2-O-methyl-rac-glycero-3-phosphocholine, wherein iodine is in the form of a radioactive isotope.
  • Radioiodinated phospholipid ether analogs have shown a remarkable ability to selectively accumulate in a variety of human and animal tumors in xenograft and spontaneous tumor rodent models. It is believed that this tumor avidity arises as a consequence of metabolic differences between tumor and corresponding normal tissues. The results of this study indicate that one factor in the tumor retention of these compounds in tumors is the length of the alkyl chain that determines their hydrophobic properties.
  • NM-404 A direct comparison between NM-404 and its predecessor, NM-324, in human PC-3 tumor bearing immune-compromised mice, revealed a dramatic enhancement in both tumor uptake and total body elimination of NM-404 relative to NM-324.
  • Cl 8 analog, NM-404 was chosen for follow-up evaluation in human lung cancer patients. Preliminary results have been extremely promising in that selective uptake and retention of the agent in tumors is accompanied by rapid clearance of background radioactivity from normal tissues, especially those in the abdomen.
  • Scheme 1 Reagents and conditions: (a) MesSiBr, CH2CI2; (b) BrMg(CH 2 )I iOTHP, Li 2 CuCU (cat), THF, -78 to 20 0 C; (c) PPTS 1 EtOH 7 40 0 C; (d) TsCI, DMAP, CH 2 CI 2 ; (e) BrMg(CHz) 3 OTHP, Li 2 CuCI 4 (cat), THF, -78 to 20 0 C; (g) BrMg(CH 2 ) 6 OTHP, U 2 CuCI 4 (cat), THF, -78 to 20 0 C.
  • FPara 1 211 The synthesis was initiated by the conversion of , ⁇ -iodobenzyl alcohol 1 1 to ysHodobenzyl bromide 1 2 as shown in Scheme 1. /7-lodobenzyl bromide was further coupled with Grignard reagent derived from THP protected 1 1 -bromoundecanol 1 3 in the presence of 0.5-0.7 mol% of Li 2 CuCU. 12-( / 0-lodophenyl)dodecanol 1 7 obtained after deprotection of the first coupling product 16 was used for the synthesis of Cl 2 iodinated phospholipids as described earlier.
  • Alcohol 1 7 also served as a starting material for the synthesis of ⁇ -iodophenyl alkanols with longer chains.
  • tosylate 1 8 with Grignard reagents made from bromides 14 and 1 5 followed by THP deprotection furnished the Cl 5 (20) and Cl 8 (22) alcohols, respectively.
  • Cl 5 (20) and Cl 8 (22) alcohols were converted into corresponding alkylphosphocholines 5 (NM-397) and 6 (NM-404) according to published procedures.
  • the avidity of PLE analogs to localize in tumors was evaluated in several animal models.
  • the PC-3 model represents a human tumor cell line that was used to determine the target (tumor) to non-target ratio of NM404 and NM412 in head to head comparison in order to select a candidate for an initial human pharmacokinetic trial in prostate cancer patients.
  • the MatLyLu (Dunning R3327 rat) model a rat prostate tumor line, was used specifically to screen 9 specific analogs prior to entering them into control for dosimetry and tumor-bearing animals for determining tumor/background ratios.
  • the Walker-256 carcinosarcoma model was used for quantitative tissue distribution purposes.
  • blood levels for 6 were 0.6 ⁇ 0.1 % of ID/g as compared to levels of 0.07 ⁇ 0.00% of ID/g for the Cl 5 (5) analog.
  • Total radioactivity levels in the thyroid were relatively low in both 5 and 6 when the extremely small mass of the gland is considered.
  • thyroid levels ranged from 26 to 54% injected dose per gram of tissue, these levels are actually quite low and represent an extremely small percentage of the injected dose when the exceedingly small mass of the organ is considered and the data are presented on a percent administered dose per organ basis.
  • Non-target tissue uptake can decrease the efficacy of radiodiagnostic imaging by creating high background activity or by causing excessive exposure of radiosensitive tissues to the injected radioactivity.
  • the Cl 8 analog 6 (NM-404) displayed a propensity to remain in the circulation much longer than the Cl 2 (4) and Cl 5 (5) analogs.
  • a longer plasma half-life may be expected to result in additional opportunities for uptake of the Cl 8 compound 6 by the tumor as it continually circulated through the vasculature.
  • This extended plasma half-life may be a result of strong binding of the probe to albumin.
  • Uptake and transport of radiolabeled PLE by plasma components may also be an important factor related to the tumor retention of these compounds.
  • increase of the chain length from C7 to Cl S results in an increase in the lipophilicity of the PLE analogs. Greater lipophilicity may increase the affinity of these compounds for the cell membrane, and may alter their binding to plasma components.
  • NM404 Uptake and transport in the circulation by endogenous lipoproteins such as LDL and HDL may also impact the biological distribution into the tumor.
  • unlabeled NM404 was subjected to independent (University of Buffalo Toxicology Research Center) acute toxicity evaluation at 1200 times the anticipated imaging mass dose in rats and rabbits. The agent was well tolerated and no acute toxicities were found at this dose level. Due to its selective tumor uptake and retention properties in a variety of rodent tumor models and subsequent excellent safety profile in rats and rabbits, NM-404 was selected to undergo initial human pharmacokinetic evaluation in non-small cell lung cancer (NSCLC) patients.
  • NSCLC non-small cell lung cancer
  • NM-404 displayed significantly lower liver, kidney and abdominal background radioactivity levels, which in addition to providing promise for lung tumor imaging, suggests further evaluation of this agent in human colorectal, pancreatic and prostate cancer patients is warranted. Moreover, a lack of urinary bladder radioactivity, suggests little renal clearance of the agent or its metabolites over the time points examined. This represents a significant advantage over 1 ⁇ F-fluorodeoxyglucose (FDG), a PET agent used routinely for tumor imaging today, which undergoes significant renal elimination, thus prohibiting imaging in the area of the prostate.
  • FDG F-fluorodeoxyglucose
  • PET imaging with ' 24 I affords over 40 times the sensitivity of planar 131 l-gamma imaging
  • PET unlike traditional gamma-camera imaging, also offers significant resolution enhancement and 3-dimensional imaging capabilities, as welj as superior quantitation properties relative to planar scintigraphic imaging.
  • the long, 4-day physical half-life of this PET isotope is well suited to the tumor uptake and retention kinetics of NM-404 and the inventors are in the process of extending the imaging studies with NM-404 to include PET.
  • NSCLC Non-small cell lung cancer
  • PLE Phospholipid ether analogs
  • phospholipids ethers are characterized by the presence of an ether-linked long chain alkyl or alkenyl alcohol connected to a glycerophosphocholine molecule ordinarily found in mammalian cells as a minor component of the total phospholipid content.
  • PLE phospholipids ethers
  • NM404 is (a) selectively retained in 27111 tumor models, including lung and brain tumors as well as PC-3 prostate bone metastases, (b) is not retained in normal, premalignant, or hyperplastic tissues and, (c) is not retained in inflammatory tissues.
  • first-in-human pharmacokinetic studies with 131 I-NM4O4 in patients with NSCLC the inventors have found that 131 I-NM4O4 is safe, and that from 24-48 hours is the optimal scintigraphic imaging time point for tumor detection. These studies also revealed significantly lower liver and background activity levels relative to earlier promising analogs and confirmed the agent doesn't cross the intact blood brain barrier.
  • NM404 be further developed for PET imaging.
  • the inventors have recently radioiodinated NM404 in excellent yield with commercial iodine-124, a relatively new, long-lived PET isotope, the half-life of which appears ideally matched to the pharmacokinetic profile of NM404.
  • Initial microPET scans obtained with 124 I-NM4O4 in xenograft and spontaneous mouse and rat tumor models confirmed universal tumor avidity and prolonged retention. Extending these results to PET scanning is now necessary in order to accurately characterize and quantitate the in vivo distribution properties of this agent.
  • the primary objective of this proposal is to further develop NM404 for PET/CT imaging in NSCLC patients with this radioisotope.
  • the present invention studies (a) the efficacy of imaging primary NSCLC tumors with 124 I-NM4O4 PET/CT in patients with NSCLC undergoing resection, by comparing pre-operative images with pathological findings, (b) the specific tumor accumulation and metabolic fate of NM404 in NSCLC patients undergoing resection, and correlate tumor retention with decreased phospholipase-D activity; (c) preliminary data regarding the sensitivity of imaging locoregional, and metastatic tumors with 124 I-NM4O4 PET/CT in patients with NSCLC, by comparing these results with FDG PET/CT scanning; and (d) preliminary data regarding the specificity of imaging with 124 I-NM4O4 PET/CT, by imaging patients who present with solitary pulmonary nodules, or have a diagnosis of pulmonary sarcoidosis or granulomatous infections such as fungal or mycobacterial infections, or bacterial pneumonias.
  • NM404 Due to its prolonged tumor retention properties, 125 l-labeled NM404 has recently afforded significant tumor regression (vide infra) in SCID mice bearing A549 human lung tumor xenografts. Exhibiting both diagnostic and therapeutic utility, NM404 is being developed as a true, potentially universal, diagnostic and therapeutic agent. [Para 145] NM404 has now been evaluated in 27 animal tumor models, including several lung, and it is clear that once the agent enters tumor cells, it reaches a metabolic dead end and becomes trapped. Prolonged tumor retention of this agent is demonstrated in a human adrenal tumor xenograft implanted into SCID mice (Fig. 14). NM404 is also retained in spontaneous murine lung tumors (Fig. 13).
  • FPara 1491 Successful Radiolabelinq of NM404 with iodine-124 (Para 1 50]
  • the inventors have obtained high specific activity sodium- lodide-124 in 0.1 N NaOH from Eastern Isotopes (Sterling, VA). Radiolabeling of NM404 is achieved in greater than 60% isolated radiochemical yield by modification of an isotope exchange method. Briefly, a 2-ml glass vial is charged with 10 mg of ammonium sulfate dissolved in 50 ⁇ l of deionized water. Glass beads are added, a Teflon lined septum and screw cap are added and the vial gently swirled.
  • a solution of 10 ⁇ g (in 10 ⁇ l of ethanol) of stock NM404 is added followed by aqueous sodium iodide-124 (1 -5 mCi) in less than 30 ⁇ l aqueous 0.01 N sodium hydroxide.
  • the reaction vile is swirled gently.
  • a 5-ml disposable syringe containing glass wool in tandem with another 5-ml charcoal nugget filled syringe with needle outlet are attached.
  • the glass wool syringe acts as a condensation chamber to catch evaporating solvents and the charcoal syringe traps free iodide/iodine.
  • the reaction vessel is heated in a heating block apparatus for 45 minutes at 1 50°C after which Four 20 ml volumes of air are injected into the reaction vial with a 25-ml disposable syringe and allowed to vent through the dual trap attachment. The temperature is raised to 160°C and the reaction vial is heated another 30 minutes. After cooling to room temperature, ethanol (200 ⁇ l) is added and the vial swirled. The ethanolic solution is passed through a pre-equilibrated Amberlite IRA 400-OH resin column to remove unreacted iodide.
  • the eluent volume is reduced to 50 ⁇ l via a nitrogen stream (use charcoal syringe trap) and the remaining volume injected onto a silica gel column (Perkin Elmer, 3 ⁇ m X 3cm disposable cartridge column eluted at 1 ml/ min with hexane/isopropanol/water (52:40:8)) for purification.
  • Final purity is determined by TLC (plastic backed silica gel-60 eluted with chloroform-methanol-water (65:35:4, Rf -0.1).
  • the HPLC solvents are removed by rotary evaporation and the resulting radioiodinated NM404 is solubilized in aqueous 2% Polysorbate-20 and passed through a 0.22 ⁇ m filter into a sterile vial. Radiochemical purity is typically greater than 99%.
  • I-NM4O4 pilot therapy study in SCID mice with human A549 lung tumor xenografts.
  • 125 I-NM4O4 was administered as either a single dose or alternatively in 3 doses (once a week for 3 weeks) to groups of 6 mice and a separate cohort received an equivalent mass dose of unlabeled NM404 for comparison.
  • Single doses were 50 or 500 ⁇ Ci and the repeat dose group received a total of 3 weekly 50 ⁇ Ci doses. Tumor growth was monitored for 10 weeks following final injection.
  • NM404 has been administered in a tracer dose for imaging (0.3 ⁇ g/kg body mass) in the Phase 1 NSCLC trial at the University of Wisconsin. A 70 kg subject would thus receive approximately 21 ⁇ g of NM404, although recent improvements in the exchange labeling procedure have resulted in much smaller mass doses being injected. Given the recent improvements in labeling and specific activity, the inventors were able to already reach a mass dose in the range of 142,9 ng/kg BW which corresponds to 0.22 nmol/kg BW or a total mass dose of 0.010 mg per 70kg patient. Further improvements are anticipated based on a new labeling methodology as described below, which will likely result in a greater than 50-fold reduction in the mass of compound required. Taken those future improvements into account, inventors believe that the intended clinical mass dose of 1-125-NM404 to be given in the clinical trials performed would be roughly 3-5 ng/kg BW or about 250 ng per 70kg patient.
  • test article was a solution of C-NM404 (active ingredient) containing inactive ingredients (vehicle).
  • the control solution for this study was the vehicle without the active ingredient.
  • test article was formulated as follows:
  • Inactive Ingredient 2% Polysorbate20 in sterile water (injection grade)
  • Inactive Ingredient 2% Polysorbate20 in sterile water
  • the test article (C-NM-404) was administered at greater than 200 times the anticipated clinical dose.
  • the control and test rats were injected intravenously in the lateral tail vein.
  • the rats were injected with the test or control article intravenously at 2 ml/kg of body weight using a 25 gauge needle and a 1 -ml syringe.
  • the injections were given by alternating rats from the control group with rats from the test group.
  • the injections were given cautiously over a 30 second to one minute time interval.
  • the injection to control rat #27-01 was given at 9:03 am and last injection to test rat #27-16 was given at 1 1 :01 am.
  • the thymus, heart, lungs, spleen, kidneys, liver, brain, and testes were collected, examined grossly, weighed and sectioned for pathology.
  • the tissue samples (except thymus) were placed in jars of "Z-fix" fixative for histology.
  • the unique numbers were also applied to each cage indicating which rats were housed within that cage. There were four cages of control rats and four cages of test rats. The rats were weighed and the mean weight of the control group was 0.234 kilograms and 0.238 kilograms for the test group. The two groups of eight (8) rats were established by random assignment. The rats were weighed daily until the termination of the study on Day 14.
  • Duration of Treatment Single dose Reference therapy, dose and mode of administration, batch number: 2% Polysorbate20 in sterile water, 2 ml/kg of body weight, given via tail vein injection over 30-60 seconds.
  • Inventors approach to the development of safe and effective cancer therapies is to design small-molecule carrier molecules which are capable of being selectively retained in cancer tissue, but not or minimally in non-cancerous tissues.
  • An extension of this approach to radiotherapy would exploit the selective delivery of the radiopharmaceutical to deposit therapeutic levels of radiation within the tumor mass while minimizing radiation-induced damage to normal tissues.
  • This technology is based on the unique biochemical and pharmacological properties of phospholipid ethers (PLE's) and especially its sub-group alkyl phosphocholine analogues, such as NM404, which displays a high degree of tumor selectivity.
  • Phospholipids are an essential component of cellular membranes where they impart structural integrity and are intimately associated with a variety of cell signaling processes.
  • Phosphatidylcholine commonly known as lecithin
  • Phospholipid ethers represent a minor subclass of phospholipids that also reside in membranes. As the name implies, these lipids contain an ether rather than an ester linkage at the C-I position.
  • Platelet-activating factor (PAF) represents one of the better known phospholipid ethers.
  • the iodinated APC analogues were readily labeled with all iodine radioisotopes using an isotope exchange method.
  • These PLE analogs are specifically designed to incorporate aromatic radioiodine in order to render the molecule stable towards in vivo deiodination.
  • the low level of thyroid activity in all prior preclinical imaging and tissue distribution studies (on both a % injected dose/g and % injected dose/organ basis) has confirmed the in vivo stability of the radioiodinated PLE analogs.
  • NM- 324 [ ⁇ -O-iodophenyO-dodecylphosphocholine], initially showed the most promise in animal tumor localization studies.
  • NM324 During initial human pharmacokinetic studies with the prototype agent, NM324, an unacceptable accumulation in liver tissue was observed and additional experiments to identify PLE compounds with superior tumor localization and background clearance properties were performed.
  • NM404 [18-(4-iodophenyl)-octadecylphosphocholine] emerged due to its enhanced ability to localize in tumor, its increased metabolic clearance from the liver, and its longer plasma half-life.
  • the lead compound NM404 has now been evaluated in over 25 animal tumor models and in every tumor model and tumor type studied so far, NM404 has shown tumor-selective retention. Prolonged tumor retention of 12S I-NM4O4 has been demonstrated in mice for periods of 20-60 days post-injection. Such very extensive and protracted tumor retention characteristics may significantly enhance the radiotherapeutic efficacy of the agent, especially for isotopes with a slow radioactive decay like e.g. iodine- 1 25.
  • NM324, the predecessor of NM404 Formal metabolism studies were conducted on several PLE analogs including NM324, the predecessor of NM404. In these studies, each agent was examined to determine their ability to serve as substrates for enzymes associated with PLE metabolism. Three major enzymatic pathways are involved in the metabolism of PLE. O-Alkyl glycerol monooxygenase (AGMO) is responsible for cleavage of the alkyl ether linkage at C-I to form either the long chain fatty alcohol or subsequently, the corresponding fatty acid. Phospholipases C (PLC) and D (PLD), on the other hand, give rise to the glycerol or phosphatidic acid products, respectively.
  • AMO O-Alkyl glycerol monooxygenase
  • Phospholipases C (PLC) and D (PLD) give rise to the glycerol or phosphatidic acid products, respectively.
  • NM324 was not a substrate for this enzyme when compared to [3H]-lyso-PAF (platelet activating factor), which was extensively metabolized.
  • [3H]-lyso-PAF platelet activating factor
  • NM324 was analyzed as a substrate for PLC isolated from Bacillus cereus and was not hydrolyzed relative to l - ⁇ almitoyl-2-pH]-palmitoyl-L-3-phosphatidylcholine (DPPC), which underwent significant hydrolysis.
  • DPPC l - ⁇ almitoyl-2-pH]-palmitoyl-L-3-phosphatidylcholine
  • PLE analogs were subjected to a phospholipase D (PLD) assay.
  • the PLD which was isolated from cabbage, is similar to mammalian PLD in that the cabbage form affords phosphatidylethanol-type products in addition to phosphatidic acid when the enzymatic reaction is performed in the presence of ethanol.
  • Several of the PLE analogs subjected to these assay conditions did give rise to the phosphatidylethanol adduct, indicating possible interaction with PLD.
  • NM404 is a metabolic substrate to human Phospholipase D, and that the relative absence of Phospholipase D in cancer cell membranes is the underlying mechanism for tumor-selective retention of NM404. Although known from the literature (reference???), it is still unclear why cancers lack PLD in their membranes.
  • NM404 precursors were also subjected to in vitro metabolism studies in various cell lines including Walker tumor cells, rat muscle (H9c2), and rat hepatocytes.
  • the extent of metabolism was determined on the basis of radiolabeled products formed after incubation for various time periods and the results normalized to cell number or the amount of cellular protein.
  • Subsequent lipid extraction of the incubation medium and cell suspension demonstrated little generation of PLE metabolites in the Walker tumor cells whereas a significant production of metabolites was seen in both the muscle cells and hepatocytes over the 48h time period studied.
  • NM404 may be due to a decrease in the membrane levels of PLD, thus precluding metabolism and clearance of NM404 from the cell.
  • PLD membrane levels of PLD
  • PLE analogs were sequestered by and subsequently metabolized by normal cells (which contained normal levels of PLD). If malignant tumor cells would possess a normal complement of PLD, that the agent would have been metabolized and eliminated from the tumor cells as well. Conversely it could be deduced that the lack of metabolism and clearance of the agent from malignant cells would support the hypothesis that these neoplastic cells lack PLD relative to surrounding normal host cells.
  • NM324 and NM404 are similar in structure to miltefosine (hexadecylphosphocholine), an antitumor ether lipid studied most extensively in Europe.
  • miltefosine hexadecylphosphocholine
  • the antitumor properties of miltefosine and several other antitumor phospholipid ether analogs have been demonstrated in a wide range of tumor cell lines including prostate-, bladder-, and terato-carcinomas, murine and human leukemias, as well as lung, colon, ovarian, brain and breast cancers (Lohmeyer M, Bittman R. Drugs of the Future 1994; 1 9: 1021 -1037).
  • these phospholipid ether analogs do not bind directly to DNA and are not mutagenic. Although the precise antiproliferative mechanism of action has not been determined, they apparently act at several tumor cell sites. These compounds have been associated with a variety of cellular effects including transport, promotion of cytokine formation, apoptosis induction, and interference with a variety of key lipid metabolism and cell signaling enzymes most of which are located in the cellular membrane. Although uncertainty remains regarding the mode of PLE uptake into cells, most evidence now supports the idea that these ether lipids are directly absorbed into cell membranes where they accumulate.
  • iodine-125 is the most appropriate radioisotope for combination with the NM404 targeting backbone, since: [Para 205] The long isotope half-life of iodine-125 matches perfectly with the long and stable tumor retention of NM404, delivering therapeutic radiation doses for an extended period of time. [Para 206] The effect of iodine-125 is caused by both, low-energy gamma/X-ray irradiation and by Auger electrons, all of which are of very limited treatment distance. Since NM404 is taken up into the tumor, iodine- 125 can effectively deliver a tumor dose but it is sparing surrounding healthy tissue.
  • Iodine-125 has Auger electrons as one of its radio-decay byproducts (Figure 2). Auger electrons cause a pronounced biological effect, but have a very short treatment distance. Since NM404 is taken up directly into all cell membranes of cancer cells (including the nuclear membrane), the treatment distance to the DNA is very low. It may effectively allow Auger electrons to be a major contributor to I-125-NM404 treatment effects.
  • the iodine-125 isotope is used for formulation in this application because it exhibits favorable characteristics for cancer radiotherapy, with properties listed below: [Para 209] Gamma Irradiation
  • 1-131 -NM404 could potentially be used for radiotherapy, the inventors believe 1-125 to be the more optimal isotope because its lower energy radiation has a shorter radiation haif distance than 1-1 31 and thus hypothesize that it will produce less damage to healthy tissue. Therefore, it was decided that 1-125-NM404 would be used for all clinical radiotherapy studies performed in order to reduce the potential for collateral damage to healthy tissues.
  • iodine-125 Due to its 60-day physical half-life and low energy 35 keV photon emission, iodine-125 is suitable for imaging experiments in mice and rats. Iodine-125 also provides therapeutic efficacy when used in permanent prostate brachytherapy implants ("brachytherapy seeds").
  • the major advantage of 1251 is that all the photons are of low energy, insuring very limited exposure of normal tissues surrounding the tumor.
  • the major difference between brachytherapy seeds containing iodine-125 and 1-125- NM404 are the effect of Auger electrons. Since brachytherapy seeds have a metal capsule around the iodine-125, only the low-energy gamma and X-ray are of therapeutic value, and Auger electrons are eliminated by the metal capsule.
  • I-125-NM404 is taken up into the cancer cell membranes (including into the nuclear membrane), so that Auger electrons can have a major contribution to the therapeutic effect.
  • iodine-131 has been used with great efficacy in the treatment of thyroid cancer, a significant disadvantage of iodine-131 is that there is a higher energy gamma emission which could actually expose adjacent surrounding tissues to more radiation than would occur with - iodine-125.
  • Units of EDE and ED are mSv/MBq or rem/mCi .
  • Phospholipid ethers can easily be labeled with iodine radioisotopes using radiolabeling methods developed in the labs.
  • the iodophenyl phospholipid ether analogs are specifically designed so that the radioiodine affixed to each molecule is stable to facile in wV ⁇ deiodination. It was found that any chemical modification of the phosphocholine moiety or shortening the chain length of the iodophenylalkyl moiety to less than 8 methylenes resulted in little or no tumor uptake.
  • the inventors have now synthesized over 20 radiolabeled PLE compounds and tested in vitro and in vivo.
  • NM-324 Clinical Evaluation of NM324: Although first generation compounds NM-324 and NM-294 displayed similar animal tumor localization characteristics, NM-324 was easier to chemically synthesize and was thus selected as the lead compound for initial clinical studies. Although images obtained in several human lung cancer patients detected tumors, images were complicated by high liver radioactivity ( Figure 3).
  • Second Generation PLE Analogs In order to decrease liver uptake and prolong the plasma phase, the inventors examined 9 structural analogs of NM-324 to identify agents that would display improved tumor- to-background tissue ratios with decreased liver uptake. The new PLE analogs were synthesized and radiolabeled with 125 I for initial image analysis in Copenhagen rats bearing Dunning R3327 prostate tumors.
  • NM-404 not only exhibited much lower liver activity than its predecessor NM324 but also maintained prolonged tumor retention (Figure 4. NM404 was therefore selected to undergo further imaging and biodistribution analysis in a variety of animal-tumor models. [Para 228] Accordingly, NM404 has now been evaluated in over 20 xenograft and spontaneous animal tumor models as shown in Table 2. In all tumor models the agent displayed significant tumor uptake and retention regardless of location. Although tumor retention can be explained by a lack of metabolic phospholipase enzymes in the tumor cell membranes, the exact mechanism of tumor cell uptake is unknown.
  • the inventors know the agent does not localize in benign intestinal adenomas (polyps) so it was desirable to further evaluate the propensity of the agent to localize in an intermediate stage of tumorigenesis, namely hyperplasia.
  • the inventors are currently evaluating the selectivity of NM404 in a unique mouse tumor model developed at Wisconsin, wherein both preneoplastic hyperplasias and malignant adenocarcinomas form spontaneously in the mammary gland. Preliminary results in this model indicate that the agent is not taken up and retained in preneoplastic lesions, and thus, appears to be exclusively retained by malignant tumor cells.
  • NM404 has been found to be retained in tumor tissue for long and extended periods of time. Tumor concentrations are almost stable for many weeks following administration of NM404, showing slow elimination from cancerous tissue over time. In contrast, NM404 is eliminated from normal tissue within a few days reaching very low levels. [Para 233] Additionally, NM404 was designed to have a long blood half life. This ensures prolonged exposure of NM404 to the tumor tissue and ensures uptake of up to 10-25% of the injected dose into the tumor tissue. The inventors believe that it is very important for a radiotherapy compound to have a large portion of the injected dose accumulating in the tissue of interest.
  • NM404 will continuously accumulate in tumor tissue over time.
  • An example of this pattern is provided in Figure 5. This may lead to a delayed onset of the therapeutic effect until the accumulation of NM404 into the tumor tissue has been continuing for several days or weeks.
  • the first-generation prototype compound NM324 was found to have a elimination half life time in plasma of 2.43 hours in rats.
  • the lead compound NM404 has a elimination half life time of roughly 209 hours in rats (distribution phase half life is 4.86 hours).
  • iodine-125 Due to its 60-day physical half-life and low energy 35 KeV photon emission, iodine-125 is suitable for imaging experiments in mice and rats. Iodine-125 also affords therapeutic characteristics.
  • Figure 6 2 nude mice were each inoculated with subcutaneous squamous cell lines SCCl and SCC6 tumor cell implants on opposing flanks. SCCI and SSC6 cells were used because one is radiosensitive relative to the other. After 14 days when the average tumor size was approaching 0.5 cm in diameter, one of the mice received 20 ⁇ Ci of I-125-NM-404 and the other one receive unlabeled NM404 in an equal mass dose.
  • A549 human non-small cell lung cancer, NSCLC, from ATCC
  • NSCLC human non-small cell lung cancer
  • Tumor cell suspension (1 xl O 6 cells in phosphate-buffered saline) is injected s.c. into the right flank of female SCID mice (6-8 weeks, CB-
  • mice 5mm in diameter, mice will be divided into groups of 6 for therapy study. fPara 2451 Dosing:
  • Tumor size measured once a week.
  • mice [Para 251 ] The tumor model used for this study was A549, a human non- small cell lung cancer, (NSCLC) obtained from ATCC (Manassas, VA). The tumor cells are maintained in Ham's F-I 2K media supplemented with 10% fetal bovine serum. Tumor cell suspension (1 x10 6 cells in phosphate- buffered saline) is injected s.c. into the right flank of female SCID mice (6-8 weeks, CB-I 7 /IcrHsd- Prkcd scld , Harlan). Animals are given free access to food and water. Upon reaching 5-10 mm in tumor diameter, mice will be enrolled in the study.
  • NSCLC human non- small cell lung cancer
  • A549 human non-small cell lung cancer, NSCLC, from ATCC
  • Ham's F- 2K media supplemented with 10% fetal bovine serum.
  • Tumor cell suspension (1 x10 6 cells in phosphate-buffered saline) is injected s.c. into the right flank of female SCID mice (6-8 weeks, CB-
  • mice 5mm in diameter, mice will be divided into groups of 6 for therapy study.
  • PC-3 human prostate cancer, from ATCC
  • Tumor cell suspension (I xI O 6 cells in phosphate-buffered saline) is injected s.c. into the right flank of male SCID mice (6-8 weeks, CB-I 7 / ⁇ crHsd- Prkcd sc/tf , Harlan),
  • mice Animals are given free access to food and water, tumor growth and animal weight are monitored. Upon reaching 4-5mm in diameter, mice will be divided into groups of 6 for therapy study. fPara 2781 Dosing:
  • Tumor size (caliper) measured once a week.
  • the present invention provides preliminary data regarding the use of the second-generation PLE analog, NM404, in imaging patients with prostate cancer.
  • This agent currently under investigation at the University of Wisconsin, is selectively retained in tumors in high levels, and has high sensitivity and specificity in preclinical models. It has passed acute toxicology testing in both rats and rabbits at >1000 times the anticipated human imaging dose, and the unlabeled agent was administered at 10 times the anticipated imaging mass dose to 10 normal volunteers at the University of Michigan and University of Wisconsin to document safety.
  • the inventors hypothesize that imaging with NM404 will ultimately prove as sensitive as imaging with FDC, will also be more specific, may afford therapeutically utility and due to its relatively long half-life be available in virtually every PET facility regardless of location.
  • NM404 has high tumor uptake
  • this agent also has the potential to be developed as a therapeutic agent when coupled with higher doses of 131 I 1 125 I, another halogen astatine, lodine-125 would be especially desirable in prostate cancer patients due to its short Auger electron path length in tissue, which would theoretically minimize radiation effects in neighboring normal tissues like the rectum.
  • the 60 day half-life of iodine- 125 matches exceedingly well with the prolonged tumor retention properties of NM404.
  • Phospholipids are an essential component of cellular membranes where they impart structural integrity and are heavily associated with a variety of cell signaling processes. Phosphatidylcholine, commonly known as lecithin, is such an example. Phospholipid ethers, on the other hand, represent a minor subclass of phospholipids that also reside in membranes.
  • NM404 12-(4-iodophenyl)- octadecylphosphocholine was selected due to its enhanced ability to localize in tumor, its increased metabolic clearance from the liver, and its longer plasma half-life.
  • lymph node metastases were clearly delineated by scintigraphy in a metastatic prostate tumor model following intravenous administration of NM404, but the tracer was not retained by uninvolved lymph nodes.
  • iodine-1 23 may also prove suitable for this agent when scanned in a 3-dimensional SPECT mode. Imaging with iodine-1 23 will require further investigation.
  • planar 2-dimensional imaging like that currently being performed with iodine-1 31 in humans, requires a delay period to allow background activity to clear from neighboring normal tissues and blood. It is likely that earlier imaging may be possible when scanning with PET and 3D SPECT where neighboring radioactivity is less interfering due to the 3- dimensional nature of these modalities. In organs where background radioactivity remains inherently low (brain for example), it may be possible to use gamma emitting isotopes like iodine-123 which in addition to providing beautiful images, would permit image acquisition later on the same day of injection.
  • PET imaging with 124 I affords over 40 times the sensitivity of planar 131 l-gamma scintigraphy.
  • PET unlike traditional gamma camera imaging, also offers significant resolution enhancement, image quantification, and 3-dimensional capabilities. Due to the preliminary success of 131 I-NM4O4 in the current lung cancer imaging trial, it is now imperative to label NM404 with iodine-1 24 and evaluate its tumor detection efficacy by PET in order to overcome the limitations inherently associated with planar scintigraphy
  • FDG-PET has paved the way for hybrid imaging, its lack of tumor cell specificity will always limit its diagnostic efficacy.
  • New molecularly targeted agents like NM404, which display universal tumor uptake and selective retention regardless of location, as well as selectivity for malignant tumor cells and not inflammatory or hyperplastic lesions, will represent a significant improvement in the detection and characterization of cancer.
  • NM404 for malignant tumors seen in mouse models
  • FDG tumor selectivity
  • the agent could be manufactured in one facility and shipped to virtually any location in the world due to its 4-day half life.
  • MicroPET images acquired 24h after iv injection of 124 I-NM4O4 were corroborated with contrast-enhanced MRI images and showed intense uptake of the tracer in the brain tumor accompanied by little or no uptake in surrounding intact brain tissue.
  • This study represents the first PET image obtained with NM404 and demonstrates the ability to efficiently radiolabel, purify, and formulate NM404 for PET imaging. These compounds may then be used for extending NM404 PET utility into human cancer patients.
  • fPara 3061 Determine tumor uptake and retention characteristics of 124 I NM404 by PET-CT in patients with radio ⁇ raphically evident metastatic prostate cancer.
  • Inclusion criteria for this study will consist of fifteen patients with metastatic prostate cancer, with at least 5 patients having soft-tissue prostate cancer metastases and at least 5 of the patients with bony metastases identifiable by conventional radiologic studies which include CT scan and bone scan. Following patient enrollment, uptake of radiolabeled NM404 will be measured by 1-1 24 isotope PET-CT scan and correlated with the radiographically evident lesions detected by the patient's other conventional staging studies.
  • Radioiodination of stable NM404 with 124 l-sodium iodide is routinely achieved by modification of an ammonium sulfate-mediated isotope exchange reaction reported by Mangner and recently optimized for NM404 in our lab.
  • Exchange reaction methodology has been used effectively for initial human trials with NM324, the predecessor of NM404 and is currently being used for preclinical studies and the human lung cancer trial. Briefly, a 2-ml glass vial is charged with 10 mg of ammonium sulfate dissolved in 50 ⁇ l of deionized water.
  • the glass wool syringe acts as a condensation chamber to catch evaporating solvents and the charcoal syringe traps free iodide/iodine.
  • the reaction vessel is heated in a heating block apparatus for 45 minutes at 1 50° C.
  • Four 20 ml volumes of air are injected into the reaction vial with a 25-ml disposable syringe and allowed to vent through the dual trap attachment.
  • the temperature is raised to 160° C and the reaction vial is heated another 30 minutes.
  • ethanol 200 ⁇ l
  • the ethanolic solution is passed through a pre-equilibrated Amberlite IRA 400-OH resin column to remove unreacted iodide.
  • the eluent volume is reduced to 50 ⁇ l via a nitrogen stream (use charcoal syringe trap) and the remaining volume injected onto an HPLC silica gel column (Perkin Elmer, 3 ⁇ m X 3cm disposable cartridge column eluted at I mI/ min with hexane/isopropanol/water (52:40:8)) for purification.
  • HPLC silica gel column Perkin Elmer, 3 ⁇ m X 3cm disposable cartridge column eluted at I mI/ min with hexane/isopropanol/water (52:40:8)
  • the HPLC solvents are removed by rotary evaporation and the resulting radioiodinated NM404 is solubilized in aqueous 2% pharmaceutical grade Polysorbate-20 (0.1 ⁇ l/mg of compound).
  • the ethanol is removed under vacuum and the residue dissolved in sterile water to give a final solution containing no more than 2- 3% Polysorbate-20. Sterilization will be achieved by filtration through a sterile 0.2 ⁇ m filter unit.
  • Each of the solutions will be tested for pyrogens using the Limulus Amebocyte Lysate test kit. This is the same procedure currently employed for the preparation, purification and sterile formulation of 1-131 -labeled NM404 for lung cancer patient studies.
  • the drug Master Formulation Card and Product Preparation Checklist are included in the supplemental section of this proposal.
  • This method has afforded sufficient pure injectable 131 I-NM4O4 for our ongoing preclinical and human trials. We have not had a labeling failure to date.
  • the 131 I-NM4O4 dose is currently calculated as follows: Animal biodistribution data is used to determine the percentage of injected dose/organ at varying time points. These animal data are extrapolated to man by means of MIRD formalism (MIRDOSE PC v3.1) using standard conversion factors for differences in organ mass and anatomy between rat and standard man, providing predicted human organ doses; Based on these predicted doses, the permissible mCi dose to be injected into humans is determined using the maximal doses legally permitted by RDRC regulations for specific human tissue as defined in the Federal Register (21 CFR Part 361.1) e.g. whole body, blood, blood forming tissues, eye lens, gonads - 3 rem/dose; any other tissue - 5 rem/dose.
  • MIRDOSE PC v3.1 MIRD formalism
  • PET-CT images (whole body and selected regional conjugate views) for the first five patients will be obtained at 48 and 96' hours post injection. PET images will be obtained using a CE Clinical CE Discovery LS PET-CT scanner utilizing appropriate attenuation correction and optimized to iodine-124. The 48 and 96 hour time points are based on preliminary data regarding optimal imaging times for 131 I-NM4O4 in lung cancer, however, the optimum tumor-targeting time for 124 I-NM4O4 in human prostate cancer is yet to be determined.
  • 124 I-NM4O4 tracer accumulation and washout will be recorded on the GE Discovery LS PET/CT scanner or on CE Advance PET scanner available at our clinic.
  • a CT scan will be performed first for patient localization and attenuation correction on the Discovery LS PET/CT scanner; a transmission scan will be performed on the Advance PET scanner.
  • Each PET scan will be corrected for random, dead time, attenuation, and scatter.
  • First scanning will be performed immediately at 48 and 96 h after the 124 I-NM4O4 injection. Whole body scans will be performed with increased scanning time over the tumor site (up to 30 min). Images will be reconstructed using an iterative OSEM reconstruction method with attenuation correction, and smoothed with a Gaussian filter.
  • ROIs will be outlined to represent various organs in the field of view (typically heart, lung, muscle, liver, stomach, spleen, intestine, kidney, and bladder). For each ROI and for each time frame, the average radioactivity concentration will be calculated and standardized uptake values (SUVs) calculated.
  • SUVs uptake values
  • the uptake rate classified as positive for lesion identification will be at most 50% versus the alternative hypothesis that the rate is greater than 50%.
  • We anticipate the uptake rate classified as positive for lesion identification will be at least 80%.
  • the one-sample binomial test with the null hypothesis that the uptake rate classified as positive is at most 50% has 85% power to detect a rate of 80% at the (onesided) 10% significance level.
  • a rate of 90% will be detected with 95% power.
  • the proportion of tumors classified as positive for lesion identification will have a standard error of at most 13% and the 90% confidence interval for the proportion will be no wider than 39%.
  • FPara 31 81 Determine the specific tumor accumulation and metabolic fate of 124 I NM404 in patients with clinically organ-confined prostate cancer who are candidates for a radical prostatectomy with bilateral pelvic lymph node dissection.
  • the prostate biopsy cores will be obtained in sextant distribution (right and left apex, mid-gland and base regions) from the prostate specimen in the OR immediately following surgical removal. These six biopsy cores will be evaluated by frozen section pathology to ensure a representative sampling of both malignant and benign prostate tissue.
  • a second set of 6 biopsy cores obtained simultaneously from the same corresponding locations in the prostate will be analyzed for uptake of 124 I-NM4O4. Accordingly, they will be photographed and undergo high-resolution scanning on a Bioscan AR2000 radioscanner and also weighed and radioactivity quantitated in a well counter. Radioactivity concentration will be determined on a %dose/g tissue sample basis for comparison. These results will be compared to histology results in order to confirm localization in tumor relative to surrounding uninvolved tissue.
  • the core biopsies will be labeled with the patient's study identification number and location in the prostate from which it was obtained.
  • the one-sided t-test with a one-sided 10% significance level has 86%, 90% and 95% power to detect the effect size of 0.8, 0.90, and 1.00, respectively.
  • the standard deviation of the tog ratio is 2.0, the 5:1 ratio in the accumulation of NM404 in the tumor to normal tissues will be detected with 86% power.
  • the standard deviation of the log ratio is 1.6, a ratio of 5: 1 in the accumulation of NM404 in tumor to normal tissues will be detected with 95% power.
  • Imaging procedures in this study will be identical to the first study, except that patients will be imaged only once, based upon the optimal imaging time as identified in the first protocol. Patients will receive an injection of 124 I-NM4O4 (0.3 ⁇ g/kg body weight, 1 mCi or the limit established by dosimetry calculations in the first set of patients). Patients will also have a PET-CT scan 48 hours after infusion. Vital signs will be obtained post 124 I-NM4O4 infusion.
  • the null hypothesis that the sensitivity of NM404 in this patient population is at most 30% will be tested against the alternative that the sensitivity is greater than 30%.
  • the one-sample binomial test has 83% power to detect a sensitivity of 75% at the one-sided 10% significance level. A sensitivity of 80% will be detected with 90% power.
  • patients with occult metastatic disease may unnecessarily undergo local treatment with associated risks of therapy.
  • patients with a rising PSA due to local recurrence in whom systemic recurrence cannot be excluded with confidence, may unnecessarily undergo hormonal ablation, which is generally not considered curative and is associated with osteoporosis development, decreased libido, weight gain, menopausal symptoms, and overall malaise, as well as the evolution of hormonally independent prostate cancer.
  • CT computed tomography
  • MRI magnetic resonance imaging
  • Radioimmunoscintigraphy with lndium-1 1 1 capromab pendetide has been utilized in patients following prostatectomy with a rising PSA who have a high clinical suspicion of occult metastatic disease and no clear evidence for metastatic disease in other imaging studies.
  • NM404 second-generation PLE analog
  • FIG. 331 Certain embodiments of the present invnetion provides preliminary data regarding the use of the second-generation PLE analog, NM404, in imaging patients with prostate cancer. It has been shown that NM404 is (a) selectively retained in a wide variety of tumor types in preclinical models, with a high degree of sensitivity, (b) is safe in humans, (c) can be radiolabeled with 1-1 24, and (d) has appropriate dosimetry characteristics labeled with 1-1 31 .
  • Radiolabeled monoclonal antibodies specific to the extracellular domain of prostate-specific membrane antigen preclinical studies in nude mice bearing LNCaP human prostate tumor, J Nucl Med.
  • Buhler KR Vessella RL. Establishment and characterization of osseous prostate cancer modelsMntra-tibial injection of human prostate cancer cells.

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Abstract

The present invention provides methods for treating, detecting and locating recurrence of cancer, radiation and chemo insensitive cancer or metastasis of cancer selected from the group consisting of Lung cancer, Adrenal cancer, Melanoma, Colon cancer, Colorectal cancer, Ovarian cancer, Prostate cancer, Liver cancer, Subcutaneous cancer, Squamous cell cancer, Intestinal cancer, Hepatocellular carcinoma, Retinoblastoma,Cervical cancer, Glioma, Breast cancer and Pancreatic cancer in subject using phospholipid ether analogs

Description

INVENTION TITLE
PHOSPHOLIPID ETHER ANALOGS AS CANCER TREATMENT AGENTS AND
METHODS THEREOF DESCRIPTION
fPara 11 RELATED APPLICATION:
[Para 2] The present application seeks priority from U.S. Provisional
Application No. 60/593,190 filed on December 20, 2004 and U.S.
Application 1 1 / , filed on December 20, 2005, all of which are incorporated herein by reference for all purposes.
fPara 31 BACKGROUND OF INVENTION:
[Para 4] The invention generally relates to phospholipids ether analogs and use thereof and specifically relates to use of phospholipid ether analogs and combinations thereof for diagnosis of metastasis, treatment, pharmacokinetic, dosimetry, and toxicity studies of various cancer types, such as non-small cell lung cancer, prostate cancer and metastasis thereof.
[Para 51 Non-small cell lung cancer (NSCLC)
[Para.6] Non-small cell lung cancer (NSCLC) is the leading cause of cancer death in the United States today. Surgical resection in appropriately selected patients offers the best chance for cure. Accurate pre-operative assessment of local, regional and distant metastatic spread is thus critical for optimal management.
[Para 7] Imaging with FDG PET scanning has recently become the "gold standard" for imaging NSCLC, due to improved sensitivity, particularly when compared with CT imaging. However, its sensitivity for identifying mediastinal lymph node involvement is only about 90%, and lack of specificity, particularly in patients with inflammatory or granulomatous disease, is particularly problematic. Furthermore, its utility in diagnosing brain tumors or metastases is limited due to high metabolic background of normal brain tissue.
[Para 8] Evaluation of the mediastinal lymph node status is essential because nodal metastasis, which occurs in nearly half of all patients with NSCLC, is probably the most frequent barrier to cure. Accurate staging may also spare patients the morbidity of unnecessary, non-curative surgical procedures. Hence, there remains a need for an imaging technique that is more sensitive, specific, and accurate than any currently available technology.
[Para 9] Current conventional modalities have limitations. Anatomic imaging with computed tomography (CT) and magnetic resonance imaging (MRI) are impractical for whole body screening but are the most widely used non-invasive imaging methods for evaluation of loco-regional spread. However, CT relies on size criteria of over one centimeter for diagnosing abnormal nodes.
(Para 10] Positron-emission tomography (PET) scanning with 18F- fluorodeoxyglucose (FDG) has generated considerable interest as an oncologic imaging technique. A recent study prospectively compared the ability of a standard approach to staging for NSCLC (CT, ultrasound, bone scanning, etc) and PET scanning to detect metastases in mediastinal lymph nodes and distant sites. Mediastinal involvement was confirmed histopathologically, and distant metastases were confirmed by other imaging tests. The sensitivity and specificity of PET for detecting mediastinal metastases were 91 % and 86%, respectively; for detecting distant metastases, 82% and 93%, respectively. This compares to sensitivity and specificity for CT scanning of mediastinal involvement 75% and 66%, respectively. A metaanalysis involving 39 studies and over 1000 patients also found that FDG- PET was more accurate than CT for mediastinal staging, (sensitivity and specificity of 85% and 90%, respectively, for FDG-PET and 61 % and 79% for CT scanning), although FDG-PET became less specific when CT showed enlarged mediastinal lymph nodes (78%).
[Para 1 1 ] FDG-PET has been shown to reduce futile thoracotomies in patients. However, because of the false positive and false negative rate, confirmatory mediastinoscopies are often recommended. For example, a retrospective study involving over 200 patients with NSCLC found that the sensitivity, specificity, positive and negative predictive values, and accuracy for FDG-PET were 64%, 77%, 45%, 88%, and 75%, respectively. [Para 1 2] FDG-PET also plays a role in diagnosing extra-thoracic disease, particularly in patients with intermediate stages of lung cancer. A study done by the American College of Physicians involving over 300 patients found that unsuspected metastatic disease or second primary malignancies was identified in 1 8 of 287 patients (6.3%). Some studies, although not all, suggest that by correctly identifying advanced disease, PET will avoid unnecessary thoracotomy on 1 in 5 patients. [Para 13] Conventional anatomic imaging techniques such as CT scanning are also poor at predicting survival following treatment. In a recent study involving 73 NSCLC patients receiving treatment with concurrent cisplatin- based chemo/radiotherapy or radiotherapy alone for advanced disease, response by conventional CT imaging did not correlate with survival. Response by FDG-PET scans, however, did correlate strongly with survival (p< 0.001 ). Survival from the date of a follow-up PET scan was 84% and 84% at 1 and 2 years respectively for 24 patients who had achieved a complete response on PET, but only 43% and 31 % of the 32 patients who did not (P=O-Ol O). These results corroborate similar findings reported recently by other authors, which also show a correlation of uptake on PET scan with biological aggressiveness of tumor, and that PET imaging late after completion of treatment is highly predictive of future survival. [Para 14] It is generally accepted that FDC-PET imaging is a poor method of identifying metastatic disease to the brain in patients with NSCLC. Under normal conditions, the gray matter of the brain has high glucose utilization and therefore the uptake of FDC is normally high. While cerebral metastatic disease is often quite metabolic and often does demonstrate increased FDG uptake, it frequently is less than the brain gray matter and therefore the cerebral metastases may not be conspicuous. In one series, the sensitivity and specificity for the identification of cerebral metastatic disease in patients with NSCLC was 60% and 99% for FDG-PET, and 100% and 100% for conventional imaging. Therefore, FDG-PET imaging is not considered to be the best method of evaluating a patient with NSCLC for metastatic disease to the brain.
[Para 1 5] Another disadvantage of FDG is that it is not specific for tumors, but accumulates in both malignant and non-malignant hypermetabolic tissues. The overwhelming majority of false positive results (positive result when the radiological abnormality is not due to cancer) with FDG-PET scans of the lung are due to inflammatory and infectious causes. FDG is a nonspecific tracer and accumulates in areas of infection or inflammation. In the lung, these areas can be localized lung parenchymal nodules or more diffuse (subsegmental, segmental or lobar) or in the hilar and mediastinal nodes. In a recent study from Japan, of 1 16 lung nodules 1 -3 cm in diameter, 1 5 out of 73 malignant nodules were false negative on FDG-PET and 1 5 out of 43 benign nodules were false positive on FDG- PET. In focal pneumonias causing ground glass opacity nodules, the false positive rate was as high as 80%. In another study, ten patients with extrapulmonary cancer had false positive FDG -PET uptake in the lung; 6 had intense focal or multifocal uptake and four had uptake in a more segmental or lobar pattern. Jn all )0 patients, the uptake was due to consolidation or atelectasis and the final diagnosis on follow-up was pulmonary inflammation or infection, In addition to active bacterial pneumonias, false positive FDC-PET results can occur in many other infectious and inflammatory conditions in the lung. In the Midwestern US, many asymptomatic people have lung nodules and enlarged nodes due to previous infection with histoplasma; although many of these nodules are quiescent, some represent smoldering or active infection. Pulmonary sarcoidosis is probably one of the more common active inflammatory granulomatous processes in the lung. Interestingly, when serial FDC-PET scans have been performed in a patient being treated with oral corticosteroids for pulmonary sarcoidosis, FDC uptake decreased and then vanished.
[Para 16] FDG-PET is also frequently negative in malignancies with a low metabolic rate, such as bronchoalveolar carcinoma or carcinoid. [Para 17] Therefore a radiopharmaceutical that could accurately identify early metastatic disease in the patients with NSCLC would have a significant impact on patient care, in terms of both staging and response to therapy. Although PET imaging has improved diagnostic efficacy in this area compared to CT, there remains a need for an accurate imaging technique that is not based upon metabolic activity, which is non-specific, but is based upon a tumor-specific function that can non-invasively screen the whole body, including the brain, TPara 181 Prostate Cancer
[Para 19] Approximately 230,1 10 new cases of prostate cancer will be diagnosed in the United States for the year 2004 alone. Despite technical refinements in definitive local treatment of clinically organ confined prostate cancer by radical prostatectomy, such that many men are cured with primary therapy alone, as many as 40% of patients will experience biochemical recurrence with long-term follow-up. This recurrence is typically defined as a post-operative PSA level which is greater than or equal to 0.4 ng/ml since patients with PSA levels above this threshold generally develop clinical evidence for recurrence within 6-49 months although a PSA level of greater than or equal to 0.2 has been proposed more recently. With limited success, clinical and pathologic criteria are currently utilized to determine the likelihood for systemic disease recurrence. Factors increasing the likelihood of systemic recurrence include a high preoperative PSA level as well as pathologic features of the surgical specimen including Cleason score >7, seminal vesicle involvement, and lymph node involvement. In contrast, extracapsular extension, positive surgical margins and Gleason score <7 are factors generally associated with local recurrence. In addition, the velocity of PSA rise following prostatectomy has been utilized to determine whether disease recurrence is local or systemic. For instance, Partin et al reported that a PSA rise of less than 0.75 ng/ml/year was more frequently associated with local recurrence. Furthermore, Patel et al reported that a PSA doubling time of greater than 12 months correlated with local recurrence. Despite these clinical and pathologic criteria, the inventors are still unable to accurately select patients appropriately for local therapy such that many men may receive unnecessary hormonal ablation.
[Para 20] One of the greatest challenges in treating patients with clinically organ confined prostate cancer or patients with biochemical recurrence following definitive treatment of presumed organ-confined disease remains to accurately distinguish localized versus metastatic disease. This diagnostic capability is important to identify patients who may benefit from effective local treatment modalities including surgery, external beam radiation, brachytherapy, and cryotherapy. Because the inventors presently do not have an accurate means of staging, patients with occult metastatic disease may unnecessarily undergo local treatment with associated risks of therapy. Furthermore, patients with a rising PSA due to local recurrence, in whom systemic recurrence cannot be excluded with confidence, may unnecessarily undergo hormonal ablation, which is generally not considered curative and is associated with osteoporosis development, decreased libido, weight gain, menopausal symptoms, and overall malaise, as well as the evolution of hormonally independent prostate cancer.
[Para 21 ] While conventional imaging studies such as computed tomography (CT) and magnetic resonance imaging (MRI) are useful in assessing soft-tissue metastasis, the vast majority of prostate cancer metastasizes to the bone only. Thus, the utility of CT and MRI scanning in assessing the disease is suboptimal and more sensitive imaging modalities for either locally recurrent or metastatic prostate cancer are necessary. Radioimmunoscintigraphy with lndium-1 1 1 capromab pendetide (ProstaScint, Cytogen Corp, Princeton, NJ) has been utilized in patients following prostatectomy with a rising PSA who have a high clinical suspicion of occult metastatic disease and no clear evidence for metastatic disease in other imaging studies. This scan is based on a radiolabeled murine monoclonal antibody which is specific for PSMA (Prostate-specific membrane antigen), a transmembrane protein which is specifically expressed by both normal and malignant prostate epithelial cells. While ProstaScint radioimmunoscintigraphy has been shown to be promising in diagnosing locally recurrent disease in the prostate bed in patients with rising PSA, clinical results for this scan have been somewhat variable, with sensitivities ranging between 44% and 92% and specificities between 36% and 86%. Furthermore, when subsequent biopsy was utilized as the standard of reference for local recurrence, false-negative ProstaScint studies have been reported in 10% to 20% of cases. In addition, false-positive uptake of ProstaScint has been reported in neurofibromatosis, lymphomas, renal carcinomas, pelvic kidneys, myolipomas, and meningiomas, as well as in the bone marrow of vertebral bodies. Given this data, use of the ProstaScint scan for patients at risk for occult metastases from prostate cancer remains controversial.
[Para 22] In patients with metastatic prostate cancer, positron-emission tomography (PET) imaging has recently been used to measure the metabolic activity of osseous metastases. This technique has proven to be effective in distinguishing active bony metastasis from osteoblast activity which occurs as a result of bone healing following successful treatment of metastatic disease. This question can be assessed better by PET than by either bone scan or CT. Furthermore, changes in PET scan findings can be seen as early as 4 weeks following initiation of systemic treatment in patients with metastatic prostate cancer, whereas in many cases no significant change is seen on conventional bone scan. Therefore imaging utilizing PET technology may be useful in monitoring response to treatment in these patients. PET scanning with 1 SF-FDC has generated considerable interest as an imaging technique. Recently, it has been shown that FDC-PET can distinguish between active and quiescent bone metastases in patients with prostate cancer. The intensity of FDG uptake is thought to reflect the metabolic and biological activity of these lesions in contrast to the traditional bone scan with technetium-diphosphonate compounds in which nonspecific osteoblast activity may be detected as a false positive signal following treatment. In addition, a false negative reading may be obtained since early metastases, which initially seed into the bone marrow, will not necessarily produce a signal until an osteoblastic response occurs. Therefore, a persistently positive bone scan does not necessarily indicate the presence of residual viable metastases and a negative bone scan result may not reflect accurately the patient's metastatic tumor burden. FDC-PET may therefore prove beneficial in guiding the management of patients with bony metastases and in a retrospective study FDC-PET and helical CT have been shown independently to be more effective than i n In-monoclonal antibody imaging in detecting metastatic disease.
[Para 23] Although the FDG-PET scan is a promising imaging technique in patients with prostate cancer, most prostate cancers are slow growing and therefore do not accumulate FDC, and thus do not image well with that agent. Furthermore, FDG is excreted in the urine and so the accumulation of FDG in the bladder will minimize the probability of detecting local recurrences of prostate cancer. Indeed Morris et al reported difficulty in detection of soft tissue metastases by FDC-PET alone when metastatic sites are obscured by anatomic pathways of tracer excretion. More recently, PET- CT has been found to be more effective than PET alone in identifying metastatic lesions in patients with suspected occult metastases. In a prospective study of patients with various tumor types, the specificity and accuracy with multiple radiologic interpretations were significantly higher for PET-CT.
[Para 24] Accordingly, the need exists for developing a more sensitive and specific imaging exam, molecular imaging agent, such as phospholipids ether compounds (PLE). It would be desirable to have tumor-selective radiopharmaceuticals, with minimal accumulation in the bladder, which could accurately identify early metastatic disease in patients with prostate cancer, would have an important impact on patient care, in terms of both staging and response to therapy.
fPara 251 SUMMARY OF THE INVENTION
[Para 26] The present invention provides a method for detecting and locating recurrence of cancer, radiation and chemo insensitive cancer or metastasis of cancer selected from the group consisting of Lung cancer, Adrenal cancer, Melanoma, Colon cancer, Colorectal cancer, Ovarian cancer, Prostate cancer, Liver cancer, Subcutaneous cancer, Squamous cell cancer, Intestinal cancer, Hepatocellular carcinoma, Retinoblastoma, Cervical cancer, Glioma, Breast cancer and Pancreatic cancer in subject that has or is suspected of having cancer. The method comprising the steps of: (a) administering a phospholipid ether analog to the subject; and (b) determining whether an organ suspected of having recurrence of cancer, radiation and chemo insensitive cancer or metastasis of cancer in the subject retains a higher level of the analog than surrounding region(s) wherein a higher retention region indicates detection and location of the recurrence of cancer, radiation insensitive and chemo cancer or metastasis of cancer. [Para 27] In a preferred embodiment, the phospholipid analog is selected from:
[Para 28] where X is selected from the group consisting of radioactive isotopes of halogen; n is an integer between 8 and 30; and Y is selected from the group comprising NH2, NR2, and NR3, wherein R is an alkyl or arylalkyl substituent or
[Para 29] where X is a radioactive isotope of halogen; n is an integer between 8 and 30; Y is selected from the group consisting of H, OH1 COOH, COOR and OR, and 2 is selected from the group consisting of NH2, NR2, and NR3, wherein R is an alkyl or arylalkyl substituent. Further, in certain embodiments, X is selected from the group of radioactive halogen isotopes consisting of 1SF1 ∞a, 76Br, 77βr, 82Br,i22|( 123I1 i24tj 125^ nη anc| 2i iAt. More preferably, the phospholipid ether isl 8-(p-lodophenyl)octadecyl phospnocboltne, 1 -O-[l 8-(p-lodophenyl)octadecyl]-l ,3-propanediol-3- phosphocholine, or 1 -O-D δ-φ-lodophenyOoctadecylJ-ii.-O-methyl-rac- glycero-3-phosphocholine, wherein iodine is in the form of a radioactive isotope. In this method, preferably the detection is carried out by a of PET, CT, MRI scanning methods combination thereof.
[Para 30] Another embodiment of the present invention provides a method for the treatment of recurrence of cancer, radiation and chemo insensitive cancer or metastasis of cancer in a subject. The method comprises administering to the subject an effective amount of a compound comprising a phospholipid ether analog, in a preferred embodiment, the recurrence of cancer, radiation and chemo insensitive cancer or metastasis of cancer occurs in the group selected from Lung cancer, Adrenal cancer, Melanoma, Colon cancer, Colorectal cancer, Ovarian cancer, Prostate cancer, Liver cancer, Subcutaneous cancer, Squamous cell cancer, Intestinal cancer, Hepatocellular carcinoma, Retinoblastoma, Cervical cancer, Glioma, Breast cancer, Pancreatic cancer and Carcinosarcoma. Also preferably, the phospholipid analog is selected from:
[Para 31 ] where X is selected from the group consisting of radioactive isotopes of halogen; n is an integer between 8 and 30; and Y is selected from the group comprising NHh1 NR2, and NR3, wherein R is an alkyl or arylalkyl substituent or
[Para 32] where X is a radioactive isotope of halogen; n is an integer between 8 and 30; Y is selected from the group consisting of H, OH, COOH, COOR and OR, and Z is selected from the group consisting of NH2, NR2, and NR3, wherein R is an alkyl or arylalkyl substituent. In this method, preferably, X is selected from the group of radioactive halogen isotopes consisting of i8F, 36Cl, 76Br, 77Br, 82βr,i22|( 123I1 124I1 125I1 m i, 2i iAt and combinations thereof. More preferably, the effective amount of phospholipid ether analog is a combination of at least two isotopes, one with one with a path range of about 0.1 A to 1 mm and a second with a path range of about 1 mm to 1 m. [Para 33] Most preferably, the effective amount of phospholipid ether analog is a combination of at least two isotopes, 125I and 131I. Also, preferably, the phospholipid ether isl 8-(p-lodophenyl)octadecyl phosphocholine, 1 -O-[l 8-(p-lodophenyl)octadecyl]-l ,3-propanediol-3- phosphocholine, or l -O-[l 8-(p-lodophenyl)octadecyl]-2~O-methyl-rac- glycero-3-phosphocholine, wherein iodine is in the form of a radioactive isotope.
[Para 34] In certain embodiments, the effective amount of phospholipid ether analog is fractionated. In yet other embodiments, the effective amount of phospholipid ether analog is about O.SμCi to about 3Ci treatable in a linear and dose dependent manner. Other embodiments provide that the dosage is adaptable to the cancer-volume. Yet other embodiments provide that the dosage for radiation insensitive tumor is greater than dosage for radiation sensitive tumor and less than 3Ci and is adaptable to the cancer- volume.
[Para 35] Another embodiment of the present invention provides the use of phospholipid ether analog for the production of a pharmaceutical composition for the treatment of recurrence of cancer, radiation and chemo insensitive cancer or metastasis of cancer. In this embodiment, the phospholipid analog is selected from:
[Para 36] where X is selected from the group consisting of radioactive isotopes of halogen; n is an integer between 8 and 30; and Y is selected from the group comprising NHz, NR2, and NR3, wherein R is an alkyl or arylalkyl substituent or
[Para 37] where X is a radioactive isotope of halogen; n is an integer between 8 and 30; Y is selected from the group consisting of H, OH, COOH, COOR and OR, and Z is selected from the group consisting of NH2, NR2, and NR3, wherein R is an alkyl or arylalkyl substituent. Also in this embodiment, preferably X is selected from the group of radioactive halogen isotopes consisting of i»Ff 36CI, 76Br, 77Br, szβr^l, '23|, w\, 12Si1 131I1 zuAt and combinations thereof. Also preferably, the phospholipid ether isl 8-(p- lodophenyOoctadecyl phosphocholine, 1 -O-D 8-(p-lodophenyl)octadecyl]- 1 ,3-propanediol-3-phosphocholine, or 1 -O-[l 8-(p-lodophenyl)octadecyl]- 2-0-methyl-rac-glycero-3-phosphocholine, wherein iodine is in the form of a radioactive isotope.
[Para 38] Further objects, features and advantages of the invention will be apparent from the following detailed description, drawings and appended claims. fPara 391 BRIEF DESCRIPTION OF DRAWINGS [Para 40] Fig.1 . Structures of certain PLE analogs [Para 41 ] Fig. 2. Classical models showing the production of bremsstrahlung, characteristic X-rays, and Auger electrons, (left) Electrons are scattered elastically and inelastically by the positively charged nucleus. The inelastically scattered electron loses energy, which appears as bremsstrahlung. Elastically scattered electrons (which include backscattered electrons) are generally scattered through larger angles than are inelastically scattered electrons, (right) An incident electron ionizes the sample atom by ejecting an electron from an inner-shell (the K shell, in this case). De- excitation, in turn, produces characteristic X-radiation (above) or an Auger electron (below). Secondary electrons are ejected with low energy from outer loosely bound electron shells, a process not shown.
[Para 42] Fig. 3. Scintigraphy of the anterior chest of Patient 03 acquired at 1 , 2, and 6 days after iv administration of 1 mCi 1 31 l-NM-324. Uptake is seen in the left lingular lung cancer (T) with increasing tumor-to-background ratios over time.
[Para 43] Fig. 4. Time course (days) of NM404 in a SCID mouse with a human RL-251 adrenal tumor (T) xenograft.
[Para 44] Fig. 5. Tissue distribution 1-1 125-NM404 in RL 251 Adrenal Cancer in SCID mice depicting that while accumulation in the tumor increased, distribution in blood, spleen and kidney reduced by days 1 through 14.
[Para 45] Fig. 6. Apparent SCCl and SCC6 tumor regression after injection of 125I-NM404. By day 41 , the tumor is significantly reduced. [Para 46] Fig. 7. The image shows one of the tumor-bearing animals treated with 250 μCi of I-125-NM404 at 4 weeks following injection. The hair above the tumor has fallen off, presumably due to the strong accumulation of radioactivity in the tumor. Additionally, the surface of the tumors appears "caved in" and shows darker areas, presumably from hemorrhage and necrosis. The figure shows the effect of 1-125-NM404 on the tumor. Although tumor size (outer dimensions) may not shrink, 1-125- NM404 causes central necrosis. The measurement method measuring outer tumor dimensions may have underestimated tumor volume response following I-125-NM404.
[Para 47] Fig. 8. PC3 human prostate cancer model implanted into SCID mouse. PC3 is known to be radiation insensitive. The curves between control (no radioactivity, cold NM404) and treatment (1-1 25-NM404) only separate about 4-5 weeks after treatment, until then the growth of the tumors looks the same indicating that: 1) NM404 takes a few days to about 1 week to fully accumulate in the tumor, and 2) the isotope 1-125 has a low radiation flow (since it has a long half life). Both factors contribute to a delayed onset of the therapy effect, at a time when all NM404 has cleared out of normal tissues.
[Para 48] Fig. 9. Scintigraphic comparison of NM404 (bottom panel) and NM324 (top panel) at 1 , 2, and 4 days in a SCID mouse with human prostate PC-3 tumor (arrow) implanted in the flank. Liver and background radioactivity are much improved with NM404.
[Para 49] Fig. 10. Tumor volume for each group was recorded over the 10-week assessment period depicting the control and dosage of 50, 1 50, 250 and 500 μCi. In this figure, control animals show rapidly growing tumors over the 10-week assessment period. This confirms that the compound itself C-NM404 has no substantial effect on tumors growth. The 50 uCi dose group did not show any difference to control animals, hence these seem to be ineffective dose levels in this animal model. However, the 1 50, 250 and 500 μCi dose groups show a substantial and prolonged treatment effect. Tumor volumes are stable and same tumors appear "collapsed" (the tumor surface has caved in). Additionally, hair above the tumors fell off confirming substantial accumulation of radioactivity in these tumors. The results show a dose-linear effect of 1-1 25-NM404 on tumor volume. [Para 50] Fig. 1 1. A549 Tumor Xenografts (I Xl O6 cells, s.c.) in Female SCID Mice Fractionated Dose (3 x 50 mCi), having 2 independent controls for each dosage. A fractionated dosing of NM404 (e.g. 3 x 50 micro-Ci versus a single dose of 150 micro-Ci) produced the same therapy effect. The fractionated dose may be safer since it is eliminated from normal tissues in between fractionated injections.
[Para 51 ] Fig. 12. A549 Large Tumors vs Small Tumors 1 50 microcuries [Para 52] Fig. 13. Bioscan image (A) obtained 4 days post I Z5|_NM404 injection into an Apc^m mouse. Digital photo (B) and positionally matched fused image (C) of excised mouse lung containing spontaneous lung tumor (2 mm dia, arrow) showing intense uptake of NM404 into the tumor. [Para 53] Fig. 14. Time course of >25I-NM4O4 in human RL-251 adrenal cancer xenograft in a SCID mouse. Prolonged tumor (1 .5x0.5 cm, arrow) retention is evident even after 20 days.
[Para 54] Fig. 1 5. Fused 3D surface-rendered MRI image (blue) and 3D , microPET image (A) obtained 24h after iv injection of 124I-NM4O4 (80 μCi) into a rat with a CNS-I glioma brain tumor. Images were fused using Amira
(v3.1). Lower panels show (B) contrast-enhanced coronal MRI slice through the tumor (arrow) and (C) fused coronal MRI and )24I-NM4O4 microPET images corroborating presence and location of the tumor.
[Para 55] Fig. 16. 124l-MicroPET images a human A549 lung tumor xenograft in a SCID mouse 48h post injection of 124I-NM4O4 (80 μCi).
Corresponding imaging planes are indicated by dashed green line in coronal view.
[Para 56] Fig. 1 7. i2Η-MicroPET "mages (coronal, sagittal, and axial) 96h after iv injection of 124I-NM4O4 into a PC-3 (flank) tumor -bearing mouse.
No bladder activity was evident at any time point
[Para 57] Fig. 18. 124l-MicroPET images of a transgenic mouse with a spontaneous c-myc pancreatic adenocarcinoma (5mm) 1 8h post injection of i 24|~NM404 (80 μCi).
[Para 58] Fig. 19. Scintigraphic comparison of NM404 (bottom panel) and
NM324 (top panel) at 1 , 2, and 4 days in a SCID mouse with human prostate
PC-3 tumor (arrow) implanted in the flank. Liver and background radioactivity are much improved with NM404.
[Para 59] Fig. 20. Time course of 125I-NM4O4 in human RL-251 adrenal cancer xenograft in a SCID mouse. Prolonged tumor (1 .5x0.5 cm, arrow) retention is evident even after 20 days.
[Para 60] Fig. 21. Photo of excised prostate/vesicular gland complex (A).
Bioscan image obtained 4 days post NM404 injection (B). Positionally matched fused photo/Bioscan image of excised prostate/vesicular gland (C) showing intense uptake of radioactivity in the prostate tumor.
[Para 61] Fig. 22. High density 3D surface-rendered microCT images of a
Nude mouse leg at various times following intratibial injection (arrow in first panel depicts injection site and direction) of 2x105 human PC3 prostate tumor cells. Tumor begins protruding through the bone by 28 days (arrow) and by 46 days the tumor has literally destroyed the tibia leaving only the fibula intact.
[Para 62] Fig. 23. Co-registered surface-rendered and coronal slice microCT image fused with positionally-matched Bioscan radionuclide scan
(color) 4 days following injection of 125l-labeled NM404. Focal NM404 activity correlates well with tibial PC3 tumor (arrow).
[Para 63] Fig. 24. Fused 3D surface-rendered MRI image (blue) and 3D microPET image (A) obtained 24h after iv injection of 124I-NM4O4 (100 μCi) into a rat with a CNS-I glioma brain tumor. Images were fused using Amira (v3.1 ). Right panels show (B) contrast-enhanced coronal MRI slice through the tumor (arrow) and (C) fused coronal MRI and 124I-NM4O4 microPET images corroborating presence and location of the tumor.
[Para 64] Fig. 25. Posterior whole body planar nuclear medicine image (A) 4 days after iv administration of 13'l-NM-404 (0.8 mCi) to a patient with non small cell lung cancer (6 cm dia., arrow). Lung tumor is easily detected in corresponding axial (B) and coronal (C) computed tomography (CT) scans.
fPara 651 I. GENERAL DESCRIPTION OF THE INVENTION [Para 66] General Description of the Invention: Before the present methods are described, it is understood that this invention is not limited to the particular methodology, protocols, cell lines, and reagents described, as these may vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to limit the scope of the present invention which will be limited only by the appended claims.
[Para 67] It must be noted that as used herein and in the appended claims, the singular forms "a", "an", and "the" include plural reference unless the context clearly dictates otherwise. Thus, for example, reference to "a cell" includes a plurality of such cells and equivalents thereof known to those skilled in the art, and so forth. As well, the terms "a" (or "an"), "one or more" and "at least one" can be used interchangeably herein. It is also to be noted that the terms "comprising", "including", and "having" can be used interchangeably.
[Para 68] Unless defined otherwise, all technical and scientific terms used herein have the same meanings as commonly understood by one of ordinary skill in the art to which this invention belongs. Although any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, the preferred methods and materials are now described. All publications mentioned herein are incorporated herein by reference for the purpose of describing and disclosing the chemicals, cell lines, vectors, animals, instruments, statistical analysis and methodologies which are reported in the publications which might be used in connection with the invention. Nothing herein is to be construed as an admission that the invention is not entitled to antedate such disclosure by virtue of prior invention.
[Para 69] As defined herein, the term "isomer" includes, but is not limited to optical isomers and analogs, structural isomers and analogs, conformational isomers and analogs, and the like. In one embodiment, this invention encompasses the use of different optical isomers of an anti-tumor compound of Formula 3A. It will be appreciated by those skilled in the art that the anti-tumor compounds useful in the present invention may contain at least one chiral center. Accordingly, the compounds used in the methods of the present invention may exist in, and be isolated in, optically-active or racemic forms. Some compounds may also exhibit polymorphism. [Para 70] It is to be understood that the present invention may encompass the use of any racemic, optically-active, polymorphic, or stereroisomeric form, or mixtures thereof, which form possesses properties useful in the treatment of tumor-related conditions described and claimed herein. In one embodiment, the anti-tumor compounds may include pure (R)-isomers. In another embodiment, the anti-tumor compounds may include pure (S)- isomers. In another embodiment, the compounds may include a mixture of the (R) and the (S) isomers. In another embodiment, the compounds may include a racemic mixture comprising both (R) and (S) isomers. It is well known in the art how to prepare optically-active forms (for example, by resolution of the racemic form by recrystallization techniques, by synthesis from optically-active starting materials, by chiral synthesis, or by chromatographic separation using a chiral stationary phase). [Para 71] The invention includes the use of pharmaceutically acceptable salts of amino-substituted compounds with organic and inorganic acids, for example, citric acid and hydrochloric acid. The invention also includes N- oxides of the amino substituents of the compounds described herein. Pharmaceutically acceptable salts can also he prepared from the phenolic compounds by treatment with inorganic bases, for example, sodium hydroxide. Also, esters of the phenolic compounds can be made with aliphatic and aromatic carboxylic acids, for example, acetic acid and benzoic acid esters. As used herein, the term "pharmaceutically acceptable salt" refers to a compound formulated from a base compound which achieves substantially the same pharmaceutical effect as the base compound. [Para 72] This invention further includes method utilizing derivatives of the anti-tumor compounds. The term "derivatives" includes but is not limited to ether derivatives, acid derivatives, amide derivatives, ester derivatives and the like. In addition, this invention further includes methods utilizing hydrates of the anti-tumor compounds. The term "hydrate" includes but is not limited to hemihydrate, monohydrate, dihydrate, trihydrate and the like. [Para 73] This invention further includes methods of utilizing metabolites of the anti-tumor compounds. The term "metabolite" means any substance produced from another substance by metabolism or a metabolic process. [Para 74] As defined herein, "contacting" means that the anti-tumor compound used in the present invention is introduced into a sample containing the receptor in a test tube, flask, tissue culture, chip, array, plate, microplate, capillary, or the like, and incubated at a temperature and time sufficient to permit binding of the anti-tumor compound to a receptor. Methods for contacting the samples with the anti-tumor compound or other specific binding components are known to those skilled in the art and may be selected depending on the type of assay protocol to be run. Incubation methods are also standard and are known to those skilled in the art. [Para 75] In another embodiment, the term "contacting" means that the anti-tumor compound used in the present invention is introduced into a patient receiving treatment, and the compound is allowed to come in contact in vivo.
[Para 76] As used herein, the term "treating" includes preventative as well as disorder remittent treatment. As used herein, the terms "reducing", "suppressing" and "inhibiting" have their commonly understood meaning of lessening or decreasing. As used herein, the term "progression" means increasing in scope or severity, advancing, growing or becoming worse. As used herein, the term "recurrence" means the return of a disease after a remission.
[Para 77] As used herein, the term "administering" refers to bringing a patient, tissue, organ or cells in contact with an anti-tumor phospholipid ether compound. As used herein, administration can be accomplished in vitro, i.e. in a test tube, or in vivo, i.e. in cells or tissues of living organisms, for example, humans. In certain embodiments, the present invention encompasses administering the compounds useful in the present invention to a patient or subject. A "patient" or "subject", used equivalently herein, refers to a mammal, preferably a human, that either: (1 ) has a disorder remediable or treatable by administration of the anti-tumor substance using a phospholipid ether compound or (2) is susceptible to a disorder that is preventable by administering the anti-tumor compound using a phospholipid ether compound
[Para 78] As used herein, "pharmaceutical composition" means therapeutically effective amounts of the anti-tumor compound having radioactivity together with suitable diluents, preservatives, solubilizers, emulsifiers, and adjuvants, collectively "pharmaceutically-acceptable carriers." As used herein, the terms "effective amount" and "therapeutically effective amount" refer to the quantity of active therapeutic agent sufficient to yield a desired therapeutic response without undue adverse side effects such as toxicity, irritation, or allergic response. The specific "effective amount" will, obviously, vary with such factors as the particular condition being treated, the physical condition of the patient, the type of animal being treated, the duration of the treatment, the nature of concurrent therapy (if any), and the specific formulations employed and the structure of the compounds or its derivatives. In this case, an amount would be deemed therapeutically effective if it resulted in one or more of the following: (a) the prevention of disease (e.g., pancreatic cancer, breast cancer); and (b) the reversal or stabilization of such disease. The optimum effective amounts can be readily determined by one of ordinary skill in the art using routine experimentation.
[Para 79] Pharmaceutical compositions are liquids or lyophilized or otherwise dried formulations and include diluents of various buffer content (e.g., Tris-HCI, acetate, phosphate), pH and ionic strength, additives such as albumin or gelatin to prevent absorption to surfaces, detergents (e.g., Tween (Polysorbate) 20, Tween 80, Pluronic F68, bile acid salts), solubilizing agents (e.g., glycerol, polyethylene glycerol), anti-oxidants (e.g., ascorbic acid, sodium metabisulfite), preservatives (e.g., Thimerosal, benzyl alcohol, parabens), bulking substances or tonicity modifiers (e.g., lactose, mannitol), covalent attachment of polymers such as polyethylene glycol to the protein, complexation with metal ions, or incorporation of the material into or onto particulate preparations of polymeric compounds such as polylactic acid, polglycolic acid, hydrogels, etc, or onto liposomes, microemulsions, micelles, unilamellar or multilamellar vesicles, erythrocyte ghosts, or spheroplasts. Such compositions will influence the physical state, solubility, stability, rate of in vivo release, and rate of in vivo clearance. Controlled or sustained release compositions include formulation in lipophilic depots (e.g., fatty acids, waxes, oils).
[Para 80] Also encompassed by the invention are methods of administering particulate compositions coated with polymers (e.g., poloxamers or poloxamines). Other embodiments of the compositions incorporate particulate forms protective coatings, protease inhibitors or permeation enhancers for various routes of administration, including topical, parenteral, pulmonary, nasal and oral. In one embodiment the pharmaceutical composition is administered parenterally, paracancerally, transmucosally, tansdermally, intramuscularly, intravenously, intradermally, subcutaneously, intraperitonealy, intraventricular^, intracraniaJly and intratumorally.
[Para 81 ] Further, as used herein "pharmaceutically acceptable carriers" are well known to those skilled in the art and include, but are not limited to, 0,0T-0.1 M and preferably 0.05M phosphate buffer or 0.9% saline. Additionally, such pharmaceutically acceptable carriers may be aqueous or non-aqueous solutions, suspensions, and emulsions. Examples of nonaqueous solvents are propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable organic esters such as ethyl oleate. Aqueous carriers include water, alcoholic/aqueous solutions, emulsions or suspensions, including saline and buffered media.
[Para 82] Parenteral vehicles include sodium chloride solution, Ringer's dextrose, dextrose and sodium chloride, lactated Ringer's and fixed oils. Intravenous vehicles include fluid and nutrient replenishers, electrolyte replenishers such as those based on Ringer's dextrose, and the like. Preservatives and other additives may also be present, such as, for example, antimicrobials, antioxidants, collating agents, inert gases and the like. [Para 83] Controlled or sustained release compositions administerable according to the invention include formulation in lipophilic depots (e.g. fatty acids, waxes, oils). Also comprehended by the invention are particulate compositions coated with polymers (e.g. poloxamers or poloxamines) and the compound coupled to antibodies directed against tissue-specific receptors, ligands or antigens or coupled to ligands of tissue-specific receptors.
[Para 84] Other embodiments of the compositions administered according to the invention incorporate particulate forms, protective coatings, protease inhibitors or permeation enhancers for various routes of administration, including parenteral, pulmonary, nasal and oral.
[Para 85] Compounds modified by the covalent attachment of water- soluble polymers such as polyethylene glycol, copolymers of polyethylene glycol and polypropylene glycol, carboxymethyl cellulose, dextran, polyvinyl alcohol, polyvinylpyrrolidone or polyproline are known to exhibit substantially longer half-lives in blood following intravenous injection than do the corresponding unmodified compounds (Abuchowski et al., 1981 ; Newmark et al., 1982; and Katre et al., 1987). Such modifications may also increase the compound's solubility in aqueous solution, eliminate aggregation, enhance the physical and chemical stability of the compound, and greatly reduce the immunogenicity and reactivity of the compound. As a result, the desired in vivo biological activity may be achieved by the administration of such polymer-compound abducts less frequently or in lower doses than with the unmodified compound. [Para 86] In yet another method according to the invention, a pharmaceutical composition can be delivered in a controlled release system. For example, the agent may be administered using intravenous infusion, an implantable osmotic pump, a transdermal patch, liposomes, or other modes of administration. In one embodiment, a pump may be used (see Langer, supra; Sefton, CRC Crit. Ref. Biomed. Eng. 14:201 (1987); Buchwald et al., Surgery 88:507 (1980); Saudek et al., N. Engl. J. Med. 321 :574 (1 989). In another embodiment, polymeric materials can be used. In yet another embodiment, a controlled release system can be placed in proximity to the therapeutic target, for example liver, thus requiring only a fraction of the systemic dose (see, e.g., Goodson, in Medical Applications of Controlled Release, supra, vol. 2, pp. 1 1 5-1 38 (1984). Other controlled release systems are discussed in the review by Langer (Science 249:1 527-1 533 (1990). [Para 87] The pharmaceutical preparation can comprise the anti-tumor compound alone, or can further include a pharmaceutically acceptable carrier, and can be in solid or liquid form such as tablets, powders, capsules, pellets, solutions, suspensions, elixirs, emulsions, gels, creams, or suppositories, including rectal and urethral suppositories. Pharmaceutically acceptable carriers include gums, starches, sugars, cellulosic materials, and mixtures thereof. The pharmaceutical preparation containing the anti-tumor compound can be administered to a patient by, for example, subcutaneous implantation of a pellet. In a further embodiment, a pellet provides for controlled release of anti-tumor compound over a period of time. The preparation can also be administered by intravenous, intra-arterial, or intramuscular injection of a liquid preparation oral administration of a liquid or solid preparation, or by topical application, Administration can also be accomplished by use of a rectal suppository or a urethral suppository. [Para 88] The pharmaceutical preparations administerable by the invention can be prepared by known dissolving, mixing, granulating, or tablet-forming processes. For oral administration, the anti-tumor compounds or their physiologically tolerated derivatives such as salts, esters, N-oxides, and the like are mixed with additives customary for this purpose, such as vehicles, stabilizers, or inert diluents, and converted by customary methods into suitable forms for administration, such as tablets, coated tablets, hard or soft gelatin capsules, aqueous, alcoholic or oily solutions. Examples of suitable inert vehicles are conventional tablet bases such as lactose, sucrose, or cornstarch in combination with binders such as acacia, cornstarch, gelatin, with disintegrating agents such as cornstarch, potato starch, alginic acid, or with a lubricant such as stearic acid or magnesium stearate. [Para 89] Examples of suitable oily vehicles or solvents are vegetable or animal oils such as sunflower oil or fish-liver oil. Preparations can be effected both as dry and as wet granules. For parenteral administration (subcutaneous, intravenous, intra-arterial, or intramuscular injection), the anti-tumor compounds or their physiologically tolerated derivatives such as salts, esters, N-oxides, and the like are converted into a solution, suspension, or expulsion, if desired with the substances customary and suitable for this purpose, for example, solubilizers or other auxiliaries. Examples are sterile liquids such as water and oils, with or without the addition of a surfactant and other pharmaceutically acceptable adjuvants. Illustrative oils are those of petroleum, animal, vegetable, or synthetic origin, for example, peanut oil, soybean oil, or mineral oil. In general, water, saline, aqueous dextrose and related sugar solutions, and glycols such as propylene glycols or polyethylene glycol are preferred liquid carriers, particularly for injectable solutions.
[Para 90] The preparation of pharmaceutical compositions which contain an active component is well understood in the art. Such compositions may be prepared as aerosols delivered to the nasopharynx or as injectables, either as liquid solutions or suspensions; however, solid forms suitable for solution in, or suspension in, liquid prior to injection can also be prepared. The preparation can also be emulsified. The active therapeutic ingredient is , often mixed with excipients which are pharmaceutically acceptable and compatible with the active ingredient. Suitable excipients are, for example, water, saline, dextrose, glycerol, ethanol, or the like or any combination thereof.
[Para 91 ] In addition, the composition can contain minor amounts of auxiliary substances such as wetting or emulsifying agents, pH buffering agents which enhance the effectiveness of the active ingredient. [Para 92] An active component can be formulated into the composition as neutralized pharmaceutically acceptable salt forms. Pharmaceutically acceptable salts include the acid addition salts, which are formed with inorganic acids such as, for example, hydrochloric or phosphoric acids, or such organic acids as acetic, oxalic, tartaric, mandelic, and the like. Salts formed from the free carboxyl groups can also be derived from inorganic bases such as, for example, sodium, potassium, ammonium, calcium, or ferric hydroxides, and such organic bases as isopropylamine, trimethylamine, 2-ethylamino ethanol, histidine, procaine, and the like. [Para 93] For topical administration to body surfaces using, for example, creams, gels, drops, and the like, the anti-tumor compounds or their physiologically tolerated derivatives such as salts, esters, N-oxides, and the like are prepared and applied as solutions, suspensions, or emulsions in a physiologically acceptable diluent with or without a pharmaceutical carrier. [Para 94] In another method according to the invention, the active compound can be delivered in a vesicle, in particular a liposome (see Langer, Science 249:1 527-1 533 (1 990); Treat et al., in Liposomes in the Therapy of Infectious Disease and Cancer, Lopez-Berestein and Fidler (eds.), Liss, N.Y., pp. 353-365 (1989); Lopez-Berestein ibid., pp. 31 7-327; see generally ibid). [Para 95] For use in medicine, the salts of the anti-tumor compound may be pharmaceutically acceptable salts. Other salts may, however, be useful in the preparation of the compounds according to the invention or of their pharmaceutically acceptable salts. Suitable pharmaceutically acceptable salts of the compounds include acid addition salts which may, for example, be formed by mixing a solution of the compound according to the invention with a solution of a pharmaceutically acceptable acid such as hydrochloric acid, sulphuric acid, methanesulphonic acid, fumaric acid, maleic acid, succinic acid, acetic acid, benzoic acid, oxalic acid, citric acid, tartaric acid, carbonic acid or phosphoric acid.
[Para 96] Generally, phospholipids ether compounds and specially NM404 is a promising new tumor-selective diagnostic imaging agent to monitor the treatment response of several tumor treatment modalities. Radioiodinated NM404, a second-generation phospholipid ether analog, had displayed remarkable tumor selectivity in 27/27 tumor models. Due to a lack of metabolic phospholipase enzymes in the membranes of tumor cells, the prevailing hypothesis of this approach is that phospholipid ether analogs become trapped exclusively in tumor cell membranes because of their inability to become metabolized and eliminated. Thus, the differential clearance rates of phospholipid ethers from normal cells versus viable tumor cells form the basis of this concept. Results obtained in a variety tumor models indicate that NM404 is sequestered and selectively retained by viable tumor cells and localizes in both primary and metastatic lesions regardless of anatomic location including those found in lymph nodes. Unlike FDG, this agent does not localize in infectious sites. Other advantages of NM404 over FDG include the following: NM404 is selective for and retained indefinitely by malignant tumor cells whereas FDG in not selective for tumor cells and goes to infectious sites and hyperplasias (Barret's Esophagus). Further, since 124I has a 4 day physical half life it can be shipped anywhere in the world whereas FDG with its 1 10 min half-life, may have limited distribution within 200 miles of the production site. NM404 undergoes prolonged retention (not metabolized) and therefore affords a significant therapeutic potential when mated with an appropriate radioisotope like 131I whereas FDG does not possess any therapeutic potential. NM404 can be labeled with a variety of iodine isotopes expanding it versatility (diagnosis and therapy as well as a tool for experimental animal studies) whereas FDG is limited to 18F for PET scanning or potentially 19F (stable) for magnetic resonance imaging albeit at very low sensitivity levels. Regardless of its tumor targeting ability, due to its rapid metabolism in tumor cells, it has not potential for therapy. NM404 affords the potential to not only accurately predict local tumor response to various treatment modalities, but also allows detection of distant metastatic lesions in cases of sub-therapeutic primary tumor treatment. [Para 97] The PLE compounds may be designed to more accurately estimate the specificity and sensitivity of radiolabeled PLE analogs such as NM404 as an imaging agent in prostate cancer and other cancers. Based upon preclinical models, PLE analogs such as NM404 are likely to exhibit high uptake in tumors giving this agent the significant potential for use in staging, following response to therapy, or potentially as a therapeutic agent when coupled with higher doses of 131I,125!, or 211At an alpha-emitting halogen with therapeutic efficacy.
[Para 98] H. PREFERRED EMBODIMENTS
[Para 99] The present invention generally provides methods and techniques for the detection and treatment of various cancers. The present invention provides a method for detecting and locating recurrence of cancer, radiation and chemo insensitive cancer or metastasis of cancer selected from the group consisting of Lung cancer, Adrenal cancer, Melanoma, Colon cancer, Colorectal cancer, Ovarian cancer, Prostate cancer, Liver cancer, Subcutaneous cancer, Squamous cell cancer, Intestinal cancer, Hepatocellular carcinoma, Retinoblastoma, Cervical cancer, Glioma, Breast cancer and Pancreatic cancer in subject that has or is suspected of having cancer. The method comprising the steps of: (a) administering a phospholipid ether analog to the subject; and .
(b) determining whether an organ suspected of having recurrence of cancer, radiation insensitive cancer or metastasis of cancer in the subject retains a higher level of the analog than surrounding region(s) wherein a higher retention region indicates detection and location of the recurrence of cancer, radiation and chemo insensitive cancer or metastasis of cancer.
[Para 100] In a preferred embodiment, the phospholipid analog is selected from:
CH2CH2-
[Para 101 ] where X is selected from the group consisting of radioactive halogen isotopes; n is an integer between 8 and 30; and Y is selected from the group comprising NH2, NR2, and NR3, wherein R is an alkyl or arylalkyl substituent or
[Para 102] where X is a radioactive halogen isotope; n is an integer between 8 and 30; Y is selected from the group consisting of H, OH1 COOH, COOR and OR, and Z is selected from the group consisting of NH2, NR2, and NR3, wherein R is an alkyl or arylalkyl substituent. Further, in certain embodiments, X is selected from the group of radioactive halogen isotopes consisting of 1 8F, 36CI1 76Br, 77Br, 82Br,i 22|, i23|f 124|, ns\t uη ancj 211 At. More preferably, the phospholipid ether isl 8-(p-lodophenyl)octadecyl phosphocholine, 1 -O-[l δ-tø-lodophenyOoctadecylj-l ,3-propanediol-3- phosphocholine, or 1 -O-[18-(p-lodophenyl)octadecyl]-2-0~methyl-rac- glycero-3-phosphocholine, wherein iodine is in the form of a radioactive isotope. In this method, preferably the detection is carried out by PET CT MRI scanning methods and combination thereof.
[Para 103] As used herein "alkyl" group refers to a straight chain, branched or cyclic, saturated or unsaturated aliphatic hydrocarbons. The alkyl group has 1 -16 carbons, and may be unsubstituted or substituted by one or more groups selected from halogen, hydroxy, alkoxy carbonyl, amido, alkylamido, dialkylamido, nitro, amino, alkylamino, dialkylamino, carboxyl, thio and thioalkyl. A "hydroxy" group refers to an OH group. An "alkoxy" group refers to an — O-alkyl group wherein alkyl is as defined above. A "thio" group refers to an — SH group. A "thioalkyl" group refers to an -SR group wherein R is alkyl as defined above. An "amino" group refers to an -NH2 group. An "alkylamino" group refers to an — NHR group wherein R is alkyl is as defined above. A "dialkylamino" group refers to an — NRR1 group wherein R and R1 are all as defined above. An "amido" group refers to an — CONH2. An "alkylamido" group refers to an — CONHR group wherein R is alkyl is as defined above. A "dialkylamido" group refers to an — CONRR' group wherein R and R1 are alkyl as defined above. A "nitro" group refers to an NO2 group. A "carboxyl" group refers to a COOH group.
[Para 104] As used herein, "aryl" includes both carbocyclic and heterocyclic aromatic rings, both monocyclic and fused polycyclic, where the aromatic rings can be 5- or 6-membered rings. Representative monocyclic aryl groups include, but are not limited to, phenyl, furanyl, pyrrolyl, thienyl, pyridinyl, pyrimidinyl, oxazolyl, isoxazolyl, pyrazolyl, imidazolyl, thiazolyl, isothiazolyl and the like. Fused polycyclic aryl groups are those aromatic groups that include a 5- or 6-membered aromatic or heteroaromatic ring as one or more rings in a fused ring system. Representative fused polycyclic aryl groups include naphthalene, anthracene, indolizine, indole, isoindole, benzofuranr benzothiophene, indazole, benzimidazole, benzthiazole, purine, quinoline, isoquinoline, ciπnoline, phthalazine, quinazoline, quinoxaline, 1 ,8- naphthyridine, pteridine, carbazole, acridine, phenazine, phenothiazine, phenoxazine, and azulene. Further, as used herein "arylalkyl" refers to moieties, such as benzyl, wherein an aromatic is linked to an alkyl group which is linked to the indicated position in the PLE compound. [Para 105] Another embodiment of the present invention provides a method for the treatment of recurrence of cancer, radiation and chemo insensitive cancer or metastasis of cancer in a subject. The method comprises administering to the subject an effective amount of a compound comprising a phospholipid ether analog. In a preferred embodiment, the recurrence of cancer, radiation and chemo insensitive cancer or metastasis of cancer occurs in the group selected from Lung cancer, Adrenal cancer, Melanoma, Colon cancer, Colorectal cancer, Ovarian cancer, Prostate cancer, Liver cancer, Subcutaneous cancer, Squamous cell cancer, Intestinal cancer, Hepatocellular carcinoma, Retinoblastoma, Cervical cancer, Glioma, Breast cancer, Pancreatic cancer and Carcinosarcoma. Also preferably, the phospholipid analog is selected from:
o
[Para 106] where X is selected from the group consisting of radioactive halogen isotopes halogen; n is an integer between 8 and 30; and Y is selected from the group comprising NHa, NR2, and NR3, wherein R is an alkyl or arylalkyl substituent or
[Para 107] where X is a radioactive halogen isotope; n is an integer between 8 and 30; Y is selected from the group consisting of H, OH, COOH, COOR and OR, and Z is selected from the group consisting of NH2, NR2, and NR3, wherein R is an alkyl or arylalkyl substituent. In this method, preferably, X is selected from the group of radioactive halogen isotopes consisting Of 18F, 36Cl1 76Br, 77Br, 82Br1^i1 i23|f i24|( i25|( UiI1 2HAt and combinations thereof. More preferably, the effective amount of phospholipid ether analog is a combination of at least two isotopes, one with a path range of about 0.1 Λ to 1 mm and a second with a path range of about 1 mm to 1 m, also as discussed in Fig. 2.
[Para 108] Most preferably, the effective amount of phospholipid ether analog is a combination of at least two isotopes, 125I and 131I. Also, preferably, the phospholipid ether is18-(p-lodophenyl)octadecyl phosphocholine, 1 -O-[1 8-(p-lodophenyl)octadecyl]~l ,3~propanediol-3- phosphocholine, or l -O-[l 8-(p-lodophenyl)octadecyl]-2-0-methyl-rac- glycero-3-phosphocholine, wherein iodine is in the form of a radioactive isotope.
[Para Ϊ09J As shown in Fig. 1 1 in certain embodiments, the effective amount of phospholipid ether analog is fractionated. The advantage of using fractionated dosage is that it allows for the PLE analog to be removed from normal tissues. As an example, fractionated dosing of NM404 (e.g. 3 x 50 micro-Ci versus a single dose of 150 micro-Ci) produced the same therapy effect, while still providing low doses of compounds which is then eliminated from normal tissues in between fractionated injections. [Para 1 10] In yet other embodiments, the effective amount of phospholipid ether analog is about 0.5μCi to about 50OmCi, and as shown in Fig. 10, this is treatable in a linear and dose dependent manner. Other embodiments provide that the dosage is adaptable to the cancer-volume as shown in Fig. 12. The graph in Fig. 12 shows the difference in tumor growth for the same dose of 1-125-NM404 (1 50 microCi) when injecting in animals with small (<1 cm) and large tumors (> l cm).
[Para 1 1 1 ] The results show that a small tumor showed stunned tumor growth with that dose, however a large tumor population did not show any effects for the same dose, basically behaving like non-radiated control. The graph in Fig. 1 2 further illustrates is that the effective tumor dose should be adjusted to tumor volume and that there is a tumor dose per volume of tumor volume that has to be achieved in order to show efficacy. [Para 1 12] Yet other embodiments provide that the dosage for radiation and chemo insensitive tumor is greater than dosage for radiation sensitive tumor and less than 50OmCi and is adaptable to the cancer-volume, as ascertainable by comparing PC3 (Fig. 8) and A549 cancer models (Fig. 10, 1 1 and 1 2), in which PC3 model is radiation insensitive.
[Para 1 13] Another embodiment of the present invention provides the use of phospholipid ether analog for the production of a pharmaceutical composition for the treatment of recurrence of cancer, radiation and chemo insensitive cancer or metastasis of cancer. In this embodiment, the phospholipid analog is selected from:
[Para 1 14] where X is selected from the group consisting of radioactive halogen isotopes; n is an integer between 8 and 30; and Y is selected from the group comprising NHa, NR2, and NR3, wherein R is an alkyl or arylalkyl substituent or
[Para 1 1 5] where X is a radioactive halogen isotope; n is an integer between 8 and 30; Y is selected from the group consisting of H1 OH, COOH, COOR and OR, and Z is selected from the group consisting of NH2, NR2, and NR3, wherein R is an alkyl or arylalkyl substituent. Also in this embodiment, preferably X is selected from the group of radioactive halogen isotopes
Consisting Of 1 βF, 36C|, 76βrt 77βr, 82βr( 122| r 1 23| , 124| f 1 25 | , 1 31 | t 21 1 At ancj combinations thereof. Also preferably, the phospholipid ether is18-(p- IodophenyOoctadecyl phosphocholine, 1 -O-ll 8-(p-lodophenyl)octadecyl]- 1 ,3~propanediol-3-phosphocholine, or 1 -0-[I 8-(p-lodophenyl)octadecyl]- 2-O-methyl-rac-glycero-3-phosphocholine, wherein iodine is in the form of a radioactive isotope.
fPara 1 161 Synthesis and Structure Activity Relationship Effects on the Tumor Avidity of Radioiodinated Phospholipid Ether Analogs [Para 1 1 7] Radioiodinated phospholipid ether analogs have shown a remarkable ability to selectively accumulate in a variety of human and animal tumors in xenograft and spontaneous tumor rodent models. It is believed that this tumor avidity arises as a consequence of metabolic differences between tumor and corresponding normal tissues. The results of this study indicate that one factor in the tumor retention of these compounds in tumors is the length of the alkyl chain that determines their hydrophobic properties. Decreasing the chain length from Cl 2 to C7 resulted in little or no tumor accumulation and rapid clearance of the compound in tumor-bearing rats within 24 hours of administration. Increasing the chain length had the opposite effect, with the Cl 5 and Cl 8 analogs displaying delayed plasma clearance and enhanced tumor uptake and retention in tumor-bearing rats. Tumor uptake displayed by propanediol analogs NM-412 and NM-4f3 was accompanied by high levels of liver and abdominal radioactivity 24 hours post injection to tumor bearing rats. Addition of a 2-Omethyl moiety to the propanediol backbone also retarded tumor uptake significantly. A direct comparison between NM-404 and its predecessor, NM-324, in human PC-3 tumor bearing immune-compromised mice, revealed a dramatic enhancement in both tumor uptake and total body elimination of NM-404 relative to NM-324. Based on imaging and tissue distribution studies in several rodent tumor models, the Cl 8 analog, NM-404, was chosen for follow-up evaluation in human lung cancer patients. Preliminary results have been extremely promising in that selective uptake and retention of the agent in tumors is accompanied by rapid clearance of background radioactivity from normal tissues, especially those in the abdomen. These results strongly suggest that extension of the human trials to include other cancers is warranted, especially when NM-404 is radiolabeled with iodine- 124, a new commercially available positron-emitting isotope. The relatively long physical half-life of 4 days afforded by this isotope appears well suited to the pharmacodynamic profile of NM-404. [Para 1 1 8] Chemical Synthesis. In the course of synthesis of iodinated phospholipid ether analogs the inventors sought to develop a general synthetic scheme which would allow altering the chain length in the target compounds. The synthetic approach was based on the copper catalyzed cross-coupling reaction of Grignard reagents with alkyl tosylates or halides1 5 (Schemes 1 , 2). The choice of the building blocks for alkyl chain elongation was dictated by the commercial availability of starting materials. [Para 1 1 9] Scheme 1: Reagents and conditions: (a) MesSiBr, CH2CI2; (b) BrMg(CH2)I iOTHP, Li2CuCU (cat), THF, -78 to 20 0C; (c) PPTS1 EtOH7 40 0C; (d) TsCI, DMAP, CH2CI2; (e) BrMg(CHz)3OTHP, Li2CuCI4 (cat), THF, -78 to 20 0C; (g) BrMg(CH2)6OTHP, U2CuCI4 (cat), THF, -78 to 20 0C.
19 21
8OH 20 22
FPara 1 211 The synthesis was initiated by the conversion of ,σ-iodobenzyl alcohol 1 1 to ysHodobenzyl bromide 1 2 as shown in Scheme 1. /7-lodobenzyl bromide was further coupled with Grignard reagent derived from THP protected 1 1 -bromoundecanol 1 3 in the presence of 0.5-0.7 mol% of Li2CuCU. 12-(/0-lodophenyl)dodecanol 1 7 obtained after deprotection of the first coupling product 16 was used for the synthesis of Cl 2 iodinated phospholipids as described earlier.1 2-1 3 Alcohol 1 7 also served as a starting material for the synthesis of ω-iodophenyl alkanols with longer chains. For example, coupling of the tosylate 1 8 with Grignard reagents made from bromides 14 and 1 5 followed by THP deprotection furnished the Cl 5 (20) and Cl 8 (22) alcohols, respectively. These alcohols were converted into corresponding alkylphosphocholines 5 (NM-397) and 6 (NM-404) according to published procedures.1 2-1 3 Propanediol phospholipid ethers 7 (NM-413) and 8 (NM-41 2) were synthesized from 3-benzyloxypropanol 25, and 2-0- methyl-Λ?c-glycerol phospholipid ethers 9 (NM-414) and 70 (NM-410) were obtained from l -0-benzyl-2-0-methyl-r<3c-glycerol 26 using a sequence of reactions the inventors had previously reported. Radiolabeling with iodine- 125 was accomplished by an isotope exchange method routinely employed in the laboratory.
26, R = OCH3 28, n=15, R = OCH3
29, π=18, R = H
30, n=18, R = OCH3
3 3
[Para 123] Biology. The avidity of PLE analogs to localize in tumors was evaluated in several animal models. The PC-3 model represents a human tumor cell line that was used to determine the target (tumor) to non-target ratio of NM404 and NM412 in head to head comparison in order to select a candidate for an initial human pharmacokinetic trial in prostate cancer patients. The MatLyLu (Dunning R3327 rat) model, a rat prostate tumor line, was used specifically to screen 9 specific analogs prior to entering them into control for dosimetry and tumor-bearing animals for determining tumor/background ratios. Finally, the Walker-256 carcinosarcoma model was used for quantitative tissue distribution purposes. [Para 124] In order to expedite the screening process and minimize the number of tumor bearing animals utilized in multiple time point tissue distribution studies, new radioiodinated homologs were imaged by gamma camera scintigraphy in the rat Dunning R3337 (MAT LyLu strain) prostate cancer model. Thus, male Copenhagen rats received subcutaneous injection of MAT LyLu cells (1 x 106 cells) in the thigh 10-14 days prior to injection of the radioiodinated PLE analogs (30-40 μCi) in 2% Tween-2O solution. Gamma camera images were obtained at multiple time points including 24 and 48 hours post injection. Homologs (NM-410, NM-413, and NM-414) displaying high hepatic uptake, significant abdominal accumulation and retention or poor tumor uptake and retention were not submitted to subsequent biodistribution analysis. Tissue distribution of radioactivity in rats bearing Walker-256 carcinosarcoma was assessed at various time intervals following intravenous administration of the radioiodinated chain length homologs. The first group of compounds that was tested included three alkylphosphocholines: a shorter chain analog with seven carbons 3 (NM-396) and two analogs with a longer chain length, 5 (NM-397, Cl 5 alkyl chain length) and 6 (NM-404, Cl 8 alkyl chain length). [Para 125] Initial biodistribution experiments performed with 3 (NM-396, C7 analog) indicated rapid tissue clearance accompanied by significant in wVo deiodination. By 24 hours, the amount of radioactivity in the thyroid was 21 3% ID/g, whereas levels of radioactivity in all of the organs surveyed was <0.10% ID/g. As follows from these studies, reducing the number of methylene groups to seven apparently afforded a much more hydrophilic molecule which was rapidly excreted by the kidneys. In contrast to compound 4 (NM-346, the Cl 2 analog), the C7 analog 3 cleared rapidly from the rat and did not localize in tumor tissue at any of the time points examined.
[Para 126] This observation directed the future efforts to assessing the effect of increasing the length of the aliphatic chain upon tumor uptake and retention. The tissue distribution of the Cl 5 homolσg 5 (NM-397) was assessed utilizing the same Walker 256 rat tumor model. Radioactivity in the tumor increased with time and peaked at 48 hours after administration (1.65±0.23 % of ID/g) as opposed to most normal tissues which exhibited their highest levels of radioactivity 6 hours after administration. With the exception of thyroid, the tumor had higher radioactivity concentrations at 24, 48, and 120 hours than any of the other tissues surveyed. A more rapid washout of radioactivity occurred in the normal tissues as compared to the tumor presumably due to metabolism and elimination by normal tissues. The accumulation of radioactivity in the thyroid increased throughout the course of the study suggesting the presence of a low level of in vivo deiodination. Levels of radioactivity in the duodenum were similar to those of tumor with maximum levels being observed at 48 hours after administration (1 .38±0.24 %ID/g).
[Para 127] Although limited results were obtained with the Cl 2 analog 4 (NM-346) in this model, results suggest that the tissue distribution profife of the Cl 5 analog 5 (NM-397) was similar to that observed with the Cl 2 analog 4 with the exception of a 2-fold increase in tumor uptake at 24 h. Remaining uptake and clearance in other organs and tissues was similar between the two compounds.
[Para 128] The effect of further extending the aliphatic chain to the Cl 8 analog 6 (NM-404) depicted a dynamic profile of this compound that was similar to the Cl 5 analog 5 (NM-397) as levels of radioactivity peaked in the tumor 48 hours after administration (1.14 ±0.01 % of ID/g), albeit at slightly lower levels. Quantities of radioactivity detected in liver, kidney and duodenum were significantly lower following administration of the Cl 8 compound 6 as compared to the same organs in the Cl 5 analog 5 studies. In addition, the Cl 8 analog 6 was retained in the circulation to a much greater extent than the other chain length homologs surveyed. For example, at 1 20 hours, blood levels for 6 were 0.6±0.1 % of ID/g as compared to levels of 0.07±0.00% of ID/g for the Cl 5 (5) analog. Total radioactivity levels in the thyroid were relatively low in both 5 and 6 when the extremely small mass of the gland is considered.
[Para 129] In order to examine the transport properties of PLE analogs, plasma was isolated from Walker-256 tumor-bearing rats 7 days after administration of iodine~l 25 labeled 6. The distribution of radioactivity in the plasma compartment of a rat receiving 6 (NM-404) was studied. PAGE analysis revealed that most of the circulating radioactivity (88%) was associated with the albumin fraction following administration of the Cl 8 analog 6. This finding is similar to results reported by Eibl who studied binding of phospholipid ether prototype, ET-I 8-OCH3, with serum proteins and found that the majority of the ether lipid (71%) was bound to albumin and about 6% to HDL. [Para 130] Comparative Imaging Studies. Gamma camera scintigraphy images shown in Fig. 1 9 directly compare the tumor uptake and body clearance of second generation analog 125I-NM-4O4 (6) versus its shorter chain, first generation predecessor, (2) at 1 , 2, and 4 days post administration in immune-compromised SCID mice bearing human PC-3 prostate tumor xenografts. Qualitative scintigraphic comparison of these two PLE analogs demonstrated a striking difference in tumor uptake and overall body clearance. The longer chain agent, NM-404, displays rapid tumor uptake and prolonged retention accompanied by rapid whole body elimination of radioactivity, whereas tumor uptake and body clearance are substantially delayed with NM-324, even at 4 days following administration. Significant tumor uptake and retention of Cl 8 analog NM-404 accompanied by rapid whole body elimination clearly defined the superior imaging properties of NM-404 in this model.
[Para 131 ] Extensive quantitative tissue distribution results obtained at 1 , 3, 5, and 8 days following administration of radioiodinated NM-404 in this model indicated rapid elimination of radioactivity from all normal tissues over the 8 day evaluation period. Tumor uptake, however, continued to increase up until day 5 when it reached 18% injected dose per gram of tumor. Tumor to background tissue ratios steadily increased over the course of the experiment due to prolonged retention in tumors coupled with a steady elimination from normal tissues. Tumor to background tissue ratios exceeded 4, 6.8, 23, and 9 in blood, liver, muscle, and prostate, respectively, 3 days after injection and continued to improve at 5 and 8 days. Again, although thyroid levels ranged from 26 to 54% injected dose per gram of tissue, these levels are actually quite low and represent an extremely small percentage of the injected dose when the exceedingly small mass of the organ is considered and the data are presented on a percent administered dose per organ basis.
[Para 132] Kδtting et a/ have investigated the effects of alkyl chain length on the biodistribution of three alkyl phosphocholine (APC) analogs. The Kotting study differed from the experiments in that 1 ) compounds were orally administered at concentrations thought to be cytotoxic to tumor, and 2) the C22 compound contained a double bond in the alkyl chain. Therefore direct comparisons with the work described herein cannot be made due to large dose differences and the unknown bioavailability of the oral agents. In the Kotting study, Cl 6, Cl 8 and C22 analogs were administered orally to rats bearing a methylnitrosourea-induced mammary carcinoma in daily doses of 50-1 20 ocmol/kg. As alkyl chain length increased, the observed levels of compound in kidney, liver, and lung decreased. In contrast to the tracer results obtained with the radioiodinated PLE analogs, Kotting and coworkers found that levels of APC in blood decreased with increasing chain length, while tumor levels increased with increasing chain length.1 9 It would be expected that oral administration would result in a substantial amount of degradation of the ether lipids in the Cl tract prior to absorption. [Para 1 33] Tumors were readily visualized with the Cl 2 (4), Cl 5 (5) and Cl 8 (6) alkyl phosphocholine homologs via gamma camera scintigraphy at both 24 and 48 hours after injection. Rat imaging results obtained with Cl 5 (J, NM-413) and Cl 8 (8, NM-412) propanediol analogs, on the other hand, displayed tumor uptake accompanied by high liver and abdominal radioactivity levels. Imaging results obtained with Cl 5 (9, NM-414) and Cl 8 (10, NM-410) 2-O-Me glycerol analogs in the MAT LyLu prostate model indicated high radioactivity levels in the liver and abdomen with little to no uptake of the agent into tumors. The differences in the clearance and quantity of radioactivity from non-target tissues including the liver and intestinal tract will have significant impact upon the application of radioiodinated phospholipid ether analogs as imaging agents in humans. Non-target tissue uptake can decrease the efficacy of radiodiagnostic imaging by creating high background activity or by causing excessive exposure of radiosensitive tissues to the injected radioactivity. A preliminary clinical trial with 2 (NM-324, the mefa-iodo isomer of NM-346) in cancer patients, while affording excellent tumor uptake, was limited by the radiation dosimetry associated with accumulation in non-target tissues including liver, kidneys, and bladder.
[Para 134] The strategy was to examine the alkyl portion of phospholipid ether analog structure and determine its role in tumor localization and retention. Qualitative rat whole body screening scans acquired in MAT-LyLu tumor bearing rats with radioiodinated PLE analogs with longer chain lengths revealed sufficient tumor uptake to permit detection. However, follow-up tissue distribution studies have shown that sequential increases in the chain length from Cl 2 to Cl 5 to Cl 8, resulted in a rapid decline in the amount of radioactivity detected in the non-target organs. This substantial decrease in non-target tissue activity was accompanied by a relatively small reduction in the levels of radioactivity present in the tumor. In addition, the Cl 8 analog 6 (NM-404) displayed a propensity to remain in the circulation much longer than the Cl 2 (4) and Cl 5 (5) analogs. A longer plasma half-life may be expected to result in additional opportunities for uptake of the Cl 8 compound 6 by the tumor as it continually circulated through the vasculature. This extended plasma half-life may be a result of strong binding of the probe to albumin. Uptake and transport of radiolabeled PLE by plasma components may also be an important factor related to the tumor retention of these compounds. Certainly, increase of the chain length from C7 to Cl S, results in an increase in the lipophilicity of the PLE analogs. Greater lipophilicity may increase the affinity of these compounds for the cell membrane, and may alter their binding to plasma components. Uptake and transport in the circulation by endogenous lipoproteins such as LDL and HDL may also impact the biological distribution into the tumor. [Para 135] In preparation for human clinical trials, unlabeled NM404 was subjected to independent (University of Buffalo Toxicology Research Center) acute toxicity evaluation at 1200 times the anticipated imaging mass dose in rats and rabbits. The agent was well tolerated and no acute toxicities were found at this dose level. Due to its selective tumor uptake and retention properties in a variety of rodent tumor models and subsequent excellent safety profile in rats and rabbits, NM-404 was selected to undergo initial human pharmacokinetic evaluation in non-small cell lung cancer (NSCLC) patients. Patients underwent planar gamma-camera scintigraphy after receiving an injection of 131I-NM-4O4 (<1 mCi). Preliminary human results (n = 3) demonstrated tumor uptake and prolonged retention in the primary lung tumors (Fig 25). Relative to the high liver uptake values observed with its predecessor, NM-324, however, liver and abdominal radioactivity levels were much lower with NM-404, suggesting the feasibility of evaluating this agent in other abdominal tumors including those associated with the colon, prostate, and pancreas. [Para 136] Conclusions.
[Para 137] In summary, the rationale behind the design of new iodinated PLE analogs described in this paper was exploited in an effort to evaluate the effect of chain length and other structural modifications in the hydrophobic part of the phospholipid molecule on tumor uptake and retention. Decreasing the chain length from Cl 2 to C7 resulted in little or no tumor accumulation and rapid clearance of the compound in tumor-bearing rats by 24 hours after administration. Increasing the chain length had the opposite effect, with the Cl 5 and Cl 8 analogs displaying delayed plasma clearance and enhanced tumor uptake and retention in tumor-bearing rats. Tumor uptake displayed by propanediol analogs 7 and 8 was accompanied by fairly high levels of liver and abdominal radioactivity 24 hours post injection to tumor bearing rats. Addition of a 2-O-methyl moiety in 9 and 10 retarded tumor uptake significantly. A direct comparison between NM-404 and its predecessor, NM-324, in human PC-3 bearing immune-compromised mice, revealed a dramatic enhancement in both tumor uptake and total body elimination of NM-404 relative to NM-324. NM-404 afforded superior imaging properties to the other analogs examined in several animal models, thus warranting further evaluation of this second generation PLE analog in human lung cancer patients. Preliminary clinical results in humans indicated desired tumor uptake and retention properties similar to those seen previously in animal models. In contrast to its shorter chain predecessor NM-324, however, NM-404 displayed significantly lower liver, kidney and abdominal background radioactivity levels, which in addition to providing promise for lung tumor imaging, suggests further evaluation of this agent in human colorectal, pancreatic and prostate cancer patients is warranted. Moreover, a lack of urinary bladder radioactivity, suggests little renal clearance of the agent or its metabolites over the time points examined. This represents a significant advantage over 1 δF-fluorodeoxyglucose (FDG), a PET agent used routinely for tumor imaging today, which undergoes significant renal elimination, thus prohibiting imaging in the area of the prostate. [Para 138] Because the tumor-targeting strategy of PLE analogs appears to involve selective tumor retention over time, relatively short-lived nuclides such as 18F or even 99^jc are not practical for labeling NM-404 at the current time. Given the preliminary success of 13'l-NM-404 in the current lung cancer imaging trial, it is now imperative to radiolabel NM-404 with iodine- 124, a relatively new positron-emitting isotope with a 4.2-day half-life, and to evaluate its tumor detection efficacy by PET. It has been reported that PET imaging with '24I affords over 40 times the sensitivity of planar 131l-gamma imaging, PET, unlike traditional gamma-camera imaging, also offers significant resolution enhancement and 3-dimensional imaging capabilities, as welj as superior quantitation properties relative to planar scintigraphic imaging. The long, 4-day physical half-life of this PET isotope is well suited to the tumor uptake and retention kinetics of NM-404 and the inventors are in the process of extending the imaging studies with NM-404 to include PET.
fPara 1391 Non-small cell lung cancer (NSCLC) [Para 1403 Phospholipid ether analogs (PLE) molecules have unique biochemical and pharmacological properties resulting in a high degree of tumor selectivity. Unlike FDC, which accumulates non-specifically in both malignant and non-malignant hypermetabolic tissues, the inventors have shown radioiodinated PLE analogs undergo selective retention in a wide variety of murine and human tumors in high levels, and do not accumulate in normal or inflammatory tissues. The family of phospholipids ethers (PLE) are characterized by the presence of an ether-linked long chain alkyl or alkenyl alcohol connected to a glycerophosphocholine molecule ordinarily found in mammalian cells as a minor component of the total phospholipid content. Among the PLE, there are many subtypes, one of the most extensively studied subtypes are structurally simplified alkyl phosphocholines (APC). I- 125-NM404, is part of this APC family.
[Para 141 ] The present invention teaches methodologies for preliminary human PET imaging data regarding the use of the second-generation PLE analog, NM404, in imaging patients with NSCLC. In preclinical models, the inventors have shown that NM404 is (a) selectively retained in 27111 tumor models, including lung and brain tumors as well as PC-3 prostate bone metastases, (b) is not retained in normal, premalignant, or hyperplastic tissues and, (c) is not retained in inflammatory tissues. In first-in-human pharmacokinetic studies with 131I-NM4O4 in patients with NSCLC, the inventors have found that 131I-NM4O4 is safe, and that from 24-48 hours is the optimal scintigraphic imaging time point for tumor detection. These studies also revealed significantly lower liver and background activity levels relative to earlier promising analogs and confirmed the agent doesn't cross the intact blood brain barrier.
[Para 142] Although sufficient for early human pharmacokinetic studies, the poor imaging characteristics and planar imaging nature associated with iodine-1 31 scintigraphy dictate that NM404 be further developed for PET imaging. The inventors have recently radioiodinated NM404 in excellent yield with commercial iodine-124, a relatively new, long-lived PET isotope, the half-life of which appears ideally matched to the pharmacokinetic profile of NM404. Initial microPET scans obtained with 124I-NM4O4 in xenograft and spontaneous mouse and rat tumor models confirmed universal tumor avidity and prolonged retention. Extending these results to PET scanning is now necessary in order to accurately characterize and quantitate the in vivo distribution properties of this agent. The primary objective of this proposal is to further develop NM404 for PET/CT imaging in NSCLC patients with this radioisotope.
[Para 143] The present invention studies (a) the efficacy of imaging primary NSCLC tumors with 124I-NM4O4 PET/CT in patients with NSCLC undergoing resection, by comparing pre-operative images with pathological findings, (b) the specific tumor accumulation and metabolic fate of NM404 in NSCLC patients undergoing resection, and correlate tumor retention with decreased phospholipase-D activity; (c) preliminary data regarding the sensitivity of imaging locoregional, and metastatic tumors with 124I-NM4O4 PET/CT in patients with NSCLC, by comparing these results with FDG PET/CT scanning; and (d) preliminary data regarding the specificity of imaging with 124I-NM4O4 PET/CT, by imaging patients who present with solitary pulmonary nodules, or have a diagnosis of pulmonary sarcoidosis or granulomatous infections such as fungal or mycobacterial infections, or bacterial pneumonias. [Para 144] Due to its prolonged tumor retention properties, 125l-labeled NM404 has recently afforded significant tumor regression (vide infra) in SCID mice bearing A549 human lung tumor xenografts. Exhibiting both diagnostic and therapeutic utility, NM404 is being developed as a true, potentially universal, diagnostic and therapeutic agent. [Para 145] NM404 has now been evaluated in 27 animal tumor models, including several lung, and it is clear that once the agent enters tumor cells, it reaches a metabolic dead end and becomes trapped. Prolonged tumor retention of this agent is demonstrated in a human adrenal tumor xenograft implanted into SCID mice (Fig. 14). NM404 is also retained in spontaneous murine lung tumors (Fig. 13). Using 125l-labeled NM404, the inventors have been able to image mammary and prostate tumors in mice in excess of 60 days. Prolonged tumor retention characteristics may significantly enhance the radiotherapeutic efficacy of the agent. Imaging and tissue distribution studies performed in mouse models aimed at determining the uptake characteristics in a wide variety of tumor models are summarized in Table 2.
[Para 146] Rationale for Isotope Selection for Clinical Studies. [Para 147] Although the inventors have studied NM404 labeled with iodine- 1 31 in the preliminary human pharmacokinetic studies, this is less than optimal for imaging since iodine-131 scintigraphy produces images with limited resolution and little anatomic detail. The new long-lived isotope iodine-124, however, will produce tomographic PET images which will be displayed with corresponding CT images, thereby providing significantly greater anatomic and functional detail.
[Para 148] Because the tumor-targeting strategy of PLE analogs appears to involve selective tumor retention over time, relatively short-lived nuclides such as '8F or even 99"Tc a«*e not practical for labeling NM404 at the current time. Although the use of other isotopes, including iodine-123, may ultimately prove suitable for use with NM404 in certain tumors, the current focus will develop the PET imaging capability of this agent due to the recent success of oncologic imaging using hybrid PET-CT scanners. The unsurpassed diagnostic accuracy afforded by a biochemical or functional tumor imaging PET agent combined with the precise anatomic accuracy provided by CT is now the gold standard for tumor imaging. However, it is highly advantageous to label PLE analogs with iodine-124, a relatively new PET isotope, wherein the physical half-life (4 days) matches well with PLE tumor uptake and retention kinetics. Labeling NM404 with iodine-124 represents a natural extension of the current studies with gamma-emitting nuclides due to its 4-day physical half-life. It has been shown that PET imaging with 124I affords over 40 times the sensitivity of planer 131l-gamma scintigraphy. PET, unlike traditional gamma camera imaging, not only offers significant resolution enhancement and 3-dimensional capabilities, but when used in conjunction with hybrid PET-CT also affords exquisite image quantitation due to built in attenuation correction benefits afforded by CT. Due to the preliminary success of 131I-NM4O4 in the current lung cancer imaging trial, it is now imperative to evaluate the tumor detection efficacy of NM404 labeled with iodine-124 by PET in order to overcome the limitations inherently associated with planer scintigraphy.
FPara 1491 Successful Radiolabelinq of NM404 with iodine-124 (Para 1 50] The inventors have obtained high specific activity sodium- lodide-124 in 0.1 N NaOH from Eastern Isotopes (Sterling, VA). Radiolabeling of NM404 is achieved in greater than 60% isolated radiochemical yield by modification of an isotope exchange method. Briefly, a 2-ml glass vial is charged with 10 mg of ammonium sulfate dissolved in 50 μl of deionized water. Glass beads are added, a Teflon lined septum and screw cap are added and the vial gently swirled. A solution of 10 μg (in 10 μl of ethanol) of stock NM404 is added followed by aqueous sodium iodide-124 (1 -5 mCi) in less than 30 μl aqueous 0.01 N sodium hydroxide. The reaction vile is swirled gently. A 5-ml disposable syringe containing glass wool in tandem with another 5-ml charcoal nugget filled syringe with needle outlet are attached. The glass wool syringe acts as a condensation chamber to catch evaporating solvents and the charcoal syringe traps free iodide/iodine. The reaction vessel is heated in a heating block apparatus for 45 minutes at 1 50°C after which Four 20 ml volumes of air are injected into the reaction vial with a 25-ml disposable syringe and allowed to vent through the dual trap attachment. The temperature is raised to 160°C and the reaction vial is heated another 30 minutes. After cooling to room temperature, ethanol (200 μl) is added and the vial swirled. The ethanolic solution is passed through a pre-equilibrated Amberlite IRA 400-OH resin column to remove unreacted iodide. The eluent volume is reduced to 50 μl via a nitrogen stream (use charcoal syringe trap) and the remaining volume injected onto a silica gel column (Perkin Elmer, 3 μm X 3cm disposable cartridge column eluted at 1 ml/ min with hexane/isopropanol/water (52:40:8)) for purification. Final purity is determined by TLC (plastic backed silica gel-60 eluted with chloroform-methanol-water (65:35:4, Rf -0.1). The HPLC solvents are removed by rotary evaporation and the resulting radioiodinated NM404 is solubilized in aqueous 2% Polysorbate-20 and passed through a 0.22 μm filter into a sterile vial. Radiochemical purity is typically greater than 99%.
fPara 1 511 In-vivo PET Imaging of Murine Tumors with 124I-NM4O4. [Para 1 52] Brain Tumors. The inventors evaluated the imaging characteristics of NM404 in C6 glioma (3-5mm in diameter) tumor-bearing and sham-operated rats. Tissue distribution analysis was performed with 12SI-NM4O4 at 24 and 48 hours after injection, and a separate group of animals subjected to microPET scanning at various times following injection of 124I-NM4O4. Biodistribution analysis indicated minimal NM404 radioactivity in normal brain tissue, however, tumor/ brain ratios (% injected dose/g) were 10.6, and 12,0 at 24, 48 hours, respectively. (Fig. 1 5) NM404 tumor uptake was corroborated by histology. These preliminary results obtained in a rat glioma model suggest that NM404 holds considerable promise for the detection of malignant primary and metastatic brain tumors.
[Para 1 531 Lung, Prostate, and Pancreatic Tumor Models [Para 1 54] Preliminary microPET imaging results with 124I-NM4O4 in lung, prostate and pancreatic mouse models are presented in Figs 16-1 8. A common feature in all microPET images obtained in animal models is the lack of bladder activity at any time point. Human pharmacokinetic studies have confirmed this finding as only 4% of the agent is cleared renally within 4 days of iv injection (majority is excreted via Cl route). In all cases, NM404 displayed significant tumor avidity regardless of location in the body. Tumor uptake was typically scene within 6 hours of injection, albeit tumor to background generally improved significantly over time, especially in abdominal tumors.
[Para 1 55] Stimulated by these and other observations, the inventors recently conducted a small 12SI-NM4O4 pilot therapy study in SCID mice with human A549 lung tumor xenografts. 125I-NM4O4 was administered as either a single dose or alternatively in 3 doses (once a week for 3 weeks) to groups of 6 mice and a separate cohort received an equivalent mass dose of unlabeled NM404 for comparison. Single doses were 50 or 500 μCi and the repeat dose group received a total of 3 weekly 50 μCi doses. Tumor growth was monitored for 10 weeks following final injection. Preliminary results indicate a significant regression in tumor growth at the higher dose and perhaps a similar response in the 3-dose group, although these arms of the study are still ongoing. New arms are now being initiated to cover the dose levels between the current ones in order to more accurately evalutate the therapeutic potential of this agent. Even at the 500 μCi dose none of the mice showed any signs of toxicity.
[Para 156] Clinical Trials:
[Para 157] NM404 has been administered in a tracer dose for imaging (0.3 μg/kg body mass) in the Phase 1 NSCLC trial at the University of Wisconsin. A 70 kg subject would thus receive approximately 21 μg of NM404, although recent improvements in the exchange labeling procedure have resulted in much smaller mass doses being injected. Given the recent improvements in labeling and specific activity, the inventors were able to already reach a mass dose in the range of 142,9 ng/kg BW which corresponds to 0.22 nmol/kg BW or a total mass dose of 0.010 mg per 70kg patient. Further improvements are anticipated based on a new labeling methodology as described below, which will likely result in a greater than 50-fold reduction in the mass of compound required. Taken those future improvements into account, inventors believe that the intended clinical mass dose of 1-125-NM404 to be given in the clinical trials performed would be roughly 3-5 ng/kg BW or about 250 ng per 70kg patient.
[Para 158] The subset of phospholipid ethers known as alkyl phosphocholines possesses a wide range of pharmacologic activities, having been studied extensively as anticancer and anti-leshmanial agents at micromolar concentrations in animal models and in humans. Neither the mode of uptake nor the precise mechanism(s) of action have been clearly defined, although disruption of membrane lipid metabolism is observed in tumor cell membranes. Miltefosine, hexadecyl phosphocholine, has a reported LD50 of 606 nmol/kg in rats with a maximum tolerated dose of 39 nmol/kg over a 4-week period. In human clinical trials, a daily oral dose of 1 50 mg (3 x 50mg) was well tolerated with minimal side effects (nausea and emesis) (Planting AS, Stoter G, Verweij J. European Journal of Cancer 1993; 29A: 51 8-519).
fPara 1 591 Toxicology Studies Performed for NM404
[Para 160] This section will summarize four formal GLP toxicology studies erformed usin NM404. These studies are:
[Para 161] Formal toxicology studies of NM404 in male rats and rabbits were performed at the Toxicology Research Center of the State University of New York at Buffalo under the direction of Dr. Paul Kostyniak. Drug vehicle and drug product were provided to Dr. Kostyniak by Dr. Raymond Counsell of the University of Michigan for testing as described in the appended synopses of Study 27 and Study 28. Since no significant toxic effects were noted at a dose of 4 mg/kg, which is a dose approximately 200 times the anticipated imaging dose at that time, and the inventors estimate to be approximately 2860 times the anticipated therapy dose for clinical trials under this invention. Human safety studies of unlabeled NM404 were initiated in normal male humans at a mass of 10 times the anticipated imaging dose and about 21 times the anticipated therapy dose. The results again showed no toxicity attributable to the drug substance. This toxicology study was performed under GLP conditions.
[Para 162] Subsequently, the researchers at the University of Wisconsin initiated a toxicology study of unlabeled NM404 in female rats and rabbits (Study 31 and Study 32) at the Toxicology Research Center at SUNY-Buffalo in order to expand the patient population to be studied in the NSCLC Phase 1 clinical trial. Since no significant toxic effects were noted at a dose of 0.04 mg/kg, which is a dose approximately 200 times the revised imaging mass dose at that time, and what the inventors estimate to be approximately 286 times the anticipated therapy dose for clinical trials. Human safety studies of unlabeled NM404 were initiated in normal female humans at a mass of 10 times the initially anticipated imaging dose and about 21 times the anticipated therapy dose. Again, no significant toxic effects were noted in either female rats or rabbits. This toxicology study was performed under GLP conditions.
[Para 163] The toxicology studies were planned at that time to go up to 200 times the anticipated mass dose in human trials. Since then, improvements to the exchange labeling methodology (see Method 2 in the CMC section) will likely result in a greater than 50-fold reduction in the mass of compound required for the reaction. Thus, recalculation of the toxicology dose indicated that no toxicity was evident at a dose of at least 10,000 x the anticipated clinical dose. During the Phase 1 safety, pharmacokinetic and dosimetry trials in normal volunteers and NSCLC patients, no toxicity has been observed in either the normal male (U Ml) or female (UW) volunteers or in any of the NSCLC patients participating in the study (UW). [Para 164] Control and Test Articles:
[Para 165] The test article was a solution of C-NM404 (active ingredient) containing inactive ingredients (vehicle). The control solution for this study was the vehicle without the active ingredient. [Para 166] The test article was formulated as follows:
(1 ) Active Ingredient: 2mg/mL C-NM404
(2) Inactive Ingredient: 2% Polysorbate20 in sterile water (injection grade)
[Para 167] The control was formulated as follows:
(1 ) Inactive Ingredient: 2% Polysorbate20 in sterile water
(injection grade)
[Para 168] The control and test articles were received from Raymond E. Counsell, Ph.D., Professor of Pharmacology & Medicinal Chemistry, Department of Pharmacology, The University of Michigan Medical School, Ann Arbor, Michigan on October 29, 1998. At the Toxicology Research Center at the University of Buffalo, the study test site, the four (4) vials of test articles labeled "NM-404 (2mg/ml) in 2% Polysorbate 20/Sterile Water, MAL-Vl -82" and four (4) vials of control articles labeled "Control Vehicle-2% Polysorbate 20 in Sterile Water, MAL-Vl -83" were inventoried and stored at room temperature.
[Para 169] Administration: The test article (C-NM-404) was administered at greater than 200 times the anticipated clinical dose. The control and test rats were injected intravenously in the lateral tail vein. The rats were injected with the test or control article intravenously at 2 ml/kg of body weight using a 25 gauge needle and a 1 -ml syringe. The injections were given by alternating rats from the control group with rats from the test group. The injections were given cautiously over a 30 second to one minute time interval. The injection to control rat #27-01 was given at 9:03 am and last injection to test rat #27-16 was given at 1 1 :01 am. The following rats moved during the injection and received multiple injections: control #6 (2 sites), test #9 (2 sites), test #15 (3 sites).
[Para 170] In human applications, ET-18-OCH3, a true PLE and thus more dissimiliar to NM404 than miltefosine, (edelfosine, mouse LD50 (oral) 200 mg/kg) is administered intravenously at a dose of 1 5-20 mg/kg/d at 5mg/ml in 5% HSA. The maximum tolerated dose is 50mg/kg. Adverse effects reported for this agent include pulmonary edema impaired hepatic function and hemolysis for up to 4 hours post-injection. (Berdel WE, Fink U, Rastetter/ Lipids 1987; 22:967-969). Given that the total mass dose of NM404 would be less than one ten thousandth of the daily individual dose of Miltefosine, no toxic events would be anticipated.
[Para 171 ] Study Procedures: The rats were observed by LAF personnel for signs of acute toxicity from the time of injection until 3:30 pm. The rats were weighed five (5) times a week (Monday through Friday) and their body weights, recorded in kilograms. On December 1 7, 1998 the rats were anesthetized with sodium pentobarbital (65 mg/ml, Lot#970789, Expiration Date: February 1 , 2000) administered intraperitoneally. A heart puncture was then performed using a 20 gauge needle and a 10 ml syringe to collect the blood samples for the hematological CBC and the clinical blood chemistries. The rats were exsanguinated to cause death. The thymus, heart, lungs, spleen, kidneys, liver, brain, and testes were collected, examined grossly, weighed and sectioned for pathology. The tissue samples (except thymus) were placed in jars of "Z-fix" fixative for histology.
[Para 172] Sixteen (1 6) Sprague-Dawley rats were received from Harlan- Sprague Dawley, Indianapolis, Indiana. All rats were males born on the same date and appeared to be healthy. They were housed at the Laboratory Animal Facility and given food and water ad lib. Each rat was given a two-part number starting with 27 (the study number) and followed by a 'unique' number of '01 ' to '16' (numerical). Each rat was ear punched in the right ear with the unique number of '01 ' to '16*. The control rats were numbered from #27-01 to #27-08 and the test rats were numbered from #27-09 to #27-16. The unique numbers were also applied to each cage indicating which rats were housed within that cage. There were four cages of control rats and four cages of test rats. The rats were weighed and the mean weight of the control group was 0.234 kilograms and 0.238 kilograms for the test group. The two groups of eight (8) rats were established by random assignment. The rats were weighed daily until the termination of the study on Day 14. Test product, dose and mode of administration, batch number: C-NM-404 (MAL- Vl -82) 2 mg/ml, given via tail vein injection over 30-60 seconds Duration of Treatment: Single dose Reference therapy, dose and mode of administration, batch number: 2% Polysorbate20 in sterile water, 2 ml/kg of body weight, given via tail vein injection over 30-60 seconds. [Para 173] Safety: On Day 14, the blood samples for both groups will be analyzed for hematology and clinical chemistry. Additionally, the following organs will be removed for pathology report and histology slides: brain, lung, liver, heart, kidney, spleen, and testes. A compilation chart of organ weights with organ /weight ratios for each rat may be compiled. [Para 174] Statistical methods:
[Para 175] Differences in body weights and biochemical parameters will be compared between groups using t-test. [Para 176] Safety results:
[Para 177] No unusual behavior was noted in any of the rats during this time or throughout this 14 day study. The tail vein injection sites were checked daily when the rats were weighed and no adverse tissue reactions were noted in any of the rats. The mean weights of the rats in control group and the test group were not significantly different, despite insignificant periodic day-to-day weight loss in both groups,
[Para 178] The gross examination of tissues was performed one week following sacrifice by a pathologist, at SUNY at Buffalo. No gross lesions were noted in the test or control group. The tissue sections were then analyzed by light microscopic examination. There were no changes in the histopathology of the organs examined that can be attributed to the administration of the test material. Rat #27-12 receiving the test material has a small, focal area of myocardial injury (infarct in an early stage). This change is not seen in the other section of hear that was also processed so is interpreted as being quite small. Since the lesion is not seen in other animals receiving the material, the pathologist felt it is attributable to some unexplained alteration. It is not due to infection arising from the lung since the lungs do not show histopathological change.
[Para 1 79] Results of clinical blood chemistries and hematology were checked for any obvious values that were above or below the reference range. These values resulted in a group comparison using t-test at a p- value of 0.05. T-tests were performed on: phosphorus, sodium, potassium, AST, ALT, alkaline phosphatase, globulin, A/G ratio, glucose, WBC, RBC and hemoglobin. There were no significant differences found. [Para 180] CONCLUSION: No acute toxicologic effects attributable to the test article had been found. There were no significant differences between the test and control group.
[Para 181 ] Similar tests were performed on male and female rabbits and female rats and no adverse effects attributable to the test article were noted.
[Para 182] Preclinical Pharmacology:
[Para 183] Inventors approach to the development of safe and effective cancer therapies is to design small-molecule carrier molecules which are capable of being selectively retained in cancer tissue, but not or minimally in non-cancerous tissues. An extension of this approach to radiotherapy would exploit the selective delivery of the radiopharmaceutical to deposit therapeutic levels of radiation within the tumor mass while minimizing radiation-induced damage to normal tissues. This technology is based on the unique biochemical and pharmacological properties of phospholipid ethers (PLE's) and especially its sub-group alkyl phosphocholine analogues, such as NM404, which displays a high degree of tumor selectivity. Phospholipids are an essential component of cellular membranes where they impart structural integrity and are intimately associated with a variety of cell signaling processes. Phosphatidylcholine, commonly known as lecithin, is such an example. Phospholipid ethers, on the other hand, represent a minor subclass of phospholipids that also reside in membranes. As the name implies, these lipids contain an ether rather than an ester linkage at the C-I position. Platelet-activating factor (PAF) represents one of the better known phospholipid ethers.
[Para 1 84] Based on his early findings that several animal and human tumors contained much higher concentrations of naturally occurring ether lipids in their cell membranes than normal tissue (Snyder, F. and Wood R. Cancer Res. 1968; 28:972-978, Snyder F. and Wood R. Cancer Res. 1 969; 29:251 -258), Snyder proposed that the accumulation of ether lipids in tumors arose as a result of a lower capacity of tumor cells to metabolize these lipids. The prevailing hypothesis is that phospholipid ethers become trapped in tumor membranes because of their inability to become metabolized and eliminated, likely by a phospholipase enzyme in the cell membrane. This hypothesis is supported by experiments showing that lipid extraction of tumors following administration of radioiodinated phospholipid ethers revealed only the intact agent, whereas analysis of urine and feces revealed only metabolites (Plotzke, KP, et zA.J Nud Bioi Med, 1993; 37:264- 272). Therefore, the reason tumors retain PLE is due to the differential clearance rates of PLE from normal cells versus tumor. [Para 185] Extensive structure activity relationship studies resulted in the synthesis, radiolabeling, and evaluation of over 20 phospholipid ether analogs as potential tumor-selective imaging agents. The iodinated APC analogues were readily labeled with all iodine radioisotopes using an isotope exchange method. These PLE analogs are specifically designed to incorporate aromatic radioiodine in order to render the molecule stable towards in vivo deiodination. The low level of thyroid activity in all prior preclinical imaging and tissue distribution studies (on both a % injected dose/g and % injected dose/organ basis) has confirmed the in vivo stability of the radioiodinated PLE analogs.
[Para 186] From the library of phospholipid ether (PLE) compounds, NM- 324 [^-O-iodophenyO-dodecylphosphocholine], initially showed the most promise in animal tumor localization studies. A variety of tumors including mammary, prostate, squamous cell carcinoma, ovarian, colorectal, and melanoma were successfully visualized by scintigraphy with NM324. During initial human pharmacokinetic studies with the prototype agent, NM324, an unacceptable accumulation in liver tissue was observed and additional experiments to identify PLE compounds with superior tumor localization and background clearance properties were performed. Based upon this work, NM404 [18-(4-iodophenyl)-octadecylphosphocholine] emerged due to its enhanced ability to localize in tumor, its increased metabolic clearance from the liver, and its longer plasma half-life. A key observation documented the ability of NM404 to localize in lymph node metastases, which were clearly delineated by scintigraphy in a metastatic prostate tumor model without retention in uninvolved lymph nodes. [Para 187] The lead compound NM404 has now been evaluated in over 25 animal tumor models and in every tumor model and tumor type studied so far, NM404 has shown tumor-selective retention. Prolonged tumor retention of 12SI-NM4O4 has been demonstrated in mice for periods of 20-60 days post-injection. Such very extensive and protracted tumor retention characteristics may significantly enhance the radiotherapeutic efficacy of the agent, especially for isotopes with a slow radioactive decay like e.g. iodine- 1 25.
[Para 188] Extensive biodistribution data for the prototype agent 125I- NM324 in several tumor models revealed tumor-to-blood ratios exceeding 8:1 at later post-injection times. In one such example in a rat mammary tumor model, tumor-to-normal tissue ratios reached a maximum at 96 hours with a tumor-to-blood ratio of 8.6 and tumor-to-muscle ratio of 20:1 . Moreover, the heterogeneity of biodistribution of PLE-associated radioactivity within tumor was demonstrated by microautoradiography studies showing that the PLE radioactivity resides exclusively in viable tumor cells located toward the outer regions rather than the central necrotic regions. Comparative biodistribution data for NM-324 and NM-404 have been obtained in SCID mouse prostate and A549 lung cancer tumor models. These studies revealed high tumor-to-normal tissue ratios and tumor uptake exceeding 25% of the injected dose of NM-404 within the tumor, thus supporting the desire to study the biodistribution of NM404 in humans.
fPara 1891 Mechanism of Action [Para 190] Metabolic Studies
[Para 191 ] Formal metabolism studies were conducted on several PLE analogs including NM324, the predecessor of NM404. In these studies, each agent was examined to determine their ability to serve as substrates for enzymes associated with PLE metabolism. Three major enzymatic pathways are involved in the metabolism of PLE. O-Alkyl glycerol monooxygenase (AGMO) is responsible for cleavage of the alkyl ether linkage at C-I to form either the long chain fatty alcohol or subsequently, the corresponding fatty acid. Phospholipases C (PLC) and D (PLD), on the other hand, give rise to the glycerol or phosphatidic acid products, respectively. Using a microsomal ACMO enzyme preparation, NM324 was not a substrate for this enzyme when compared to [3H]-lyso-PAF (platelet activating factor), which was extensively metabolized. In a similar fashion, NM324 was analyzed as a substrate for PLC isolated from Bacillus cereus and was not hydrolyzed relative to l -φalmitoyl-2-pH]-palmitoyl-L-3-phosphatidylcholine (DPPC), which underwent significant hydrolysis.
[Para 192] Finally, several PLE analogs were subjected to a phospholipase D (PLD) assay. The PLD, which was isolated from cabbage, is similar to mammalian PLD in that the cabbage form affords phosphatidylethanol-type products in addition to phosphatidic acid when the enzymatic reaction is performed in the presence of ethanol. Several of the PLE analogs subjected to these assay conditions did give rise to the phosphatidylethanol adduct, indicating possible interaction with PLD. The inventors believes that NM404 is a metabolic substrate to human Phospholipase D, and that the relative absence of Phospholipase D in cancer cell membranes is the underlying mechanism for tumor-selective retention of NM404. Although known from the literature (reference???), it is still unclear why cancers lack PLD in their membranes.
[Para 193] Several NM404 precursors were also subjected to in vitro metabolism studies in various cell lines including Walker tumor cells, rat muscle (H9c2), and rat hepatocytes. In these studies, the extent of metabolism was determined on the basis of radiolabeled products formed after incubation for various time periods and the results normalized to cell number or the amount of cellular protein. Subsequent lipid extraction of the incubation medium and cell suspension demonstrated little generation of PLE metabolites in the Walker tumor cells whereas a significant production of metabolites was seen in both the muscle cells and hepatocytes over the 48h time period studied. These results correlate nicely with in vivo biodistribution studies completed on all analogs. Although several studies have been completed, the role of metabolic trapping in the uptake and retention of radiolabeled PLE analogs in tumor cells is not well defined and currently remains an active area of examination. The inventors believes that NM404 can enter the cell membranes of all cells, but gets eliminated from noncancerous cells through rapid metabolism, whereas in cancer cells it gets trapped due to lack of appropriate metabolic enzymes. [Para 194] PLD Assays
[Para 195] Due to the apparent universality of tumor retention of NM404 in animal tumor models and initial corroborative results in a human lung cancer trial, the inventors have begun to investigate the mechanism of action of this agent. Although membrane metabolism of PLE analogs is regulated by a variety of phospholipases, the inventors have focused initial efforts on phospholipase D (PLD) activity, based on the hypothesis that cellular uptake and retention of NM404 is inversely related to the amount of PLD present in the tumor cell membrane relative to normal cells.
[Para 196] Because of these findings, preliminary evaluation of PLD protein activity and PLD mRNA quantification by RT-PCR assay were performed in several murine tumor cell lines, including the murine tumor cell line hepa-1 (hepatoma), CT26 (colorectal adenocarcinoma), and TS/A (breast adenocarcinoma) and compared to normal liver. These experiments revealed that both PLD protein activity and mRNA levels were significantly lower in tumor than in normal liver tissue (p<0.05, T-test) (Table 1 ) [Para 197] Table 1 : PLD protein activity and PLD mRNA quantification for three cancer cell lines and normal liver tissue
[Para 198] As conclusion, the mechanism of selective retention of NM404 may be due to a decrease in the membrane levels of PLD, thus precluding metabolism and clearance of NM404 from the cell. Recall that earlier enzyme substrate assays conducted with PLD derived from cabbage indicated that NM404 was indeed a good substrate for this enzyme. This supports the finding of the in vitro cell culture uptake and retention study wherein it was shown that PLE analogs were sequestered by and subsequently metabolized by normal cells (which contained normal levels of PLD). If malignant tumor cells would possess a normal complement of PLD, that the agent would have been metabolized and eliminated from the tumor cells as well. Conversely it could be deduced that the lack of metabolism and clearance of the agent from malignant cells would support the hypothesis that these neoplastic cells lack PLD relative to surrounding normal host cells.
fPara 1991 Other studies
[Para 200] Mechanistic Studies with PLE Analogs: NM324 and NM404 are similar in structure to miltefosine (hexadecylphosphocholine), an antitumor ether lipid studied most extensively in Europe. The antitumor properties of miltefosine and several other antitumor phospholipid ether analogs have been demonstrated in a wide range of tumor cell lines including prostate-, bladder-, and terato-carcinomas, murine and human leukemias, as well as lung, colon, ovarian, brain and breast cancers (Lohmeyer M, Bittman R. Drugs of the Future 1994; 1 9: 1021 -1037). In contrast to many anticancer drugs, these phospholipid ether analogs do not bind directly to DNA and are not mutagenic. Although the precise antiproliferative mechanism of action has not been determined, they apparently act at several tumor cell sites. These compounds have been associated with a variety of cellular effects including transport, promotion of cytokine formation, apoptosis induction, and interference with a variety of key lipid metabolism and cell signaling enzymes most of which are located in the cellular membrane. Although uncertainty remains regarding the mode of PLE uptake into cells, most evidence now supports the idea that these ether lipids are directly absorbed into cell membranes where they accumulate. A widespread belief is that these agents act by perturbing membrane phospholipid metabolism; however, cellular distribution studies with these agents have been limited by spontaneous cellular compartmental redistribution during homogenization and subcellular fractionation procedures. In contrast to the tracer imaging doses (several μg) employed in the imaging and biodistribution studies cited by The inventors, antitumor effects are seen only at doses generally exceeding 150 mg per day (Planting AS, Stoter G, Verweij J. European Journal of Cancer, 1993; 29A:518-9; Verweij J, Planting A1 van der Burg M, Stoter G. Journal of Cancer Research & Clinical Oncology, 1992; 1 1 8:606-8; Muschiol C, et al. Lipids 1987; 22:930-934).
[Para 2011 Mechansim of Action
[Para 202] The prevailing mechanism for action is that phospholipid ethers such as NM404 become trapped in malignant tumor cell membranes because of their inability to become metabolized and eliminated. Extraction of tumors following administration of radioiodinated phospholipid ethers showed the presence of only the intact agent, whereas analysis of the urine and feces revealed only metabolites. Thus, it is the differential clearance rates of phospholipid ethers from normal cells versus tumor cells that form the basis of this concept. Preliminary results obtained in over 27 xenograft and spontaneous tumor models have universally shown NM404 to undergo selective and prolonged retention in tumors.
fPara 2031 Isotope Selection for Therapy [Para 204] The inventors believe that iodine-125 is the most appropriate radioisotope for combination with the NM404 targeting backbone, since: [Para 205] The long isotope half-life of iodine-125 matches perfectly with the long and stable tumor retention of NM404, delivering therapeutic radiation doses for an extended period of time. [Para 206] The effect of iodine-125 is caused by both, low-energy gamma/X-ray irradiation and by Auger electrons, all of which are of very limited treatment distance. Since NM404 is taken up into the tumor, iodine- 125 can effectively deliver a tumor dose but it is sparing surrounding healthy tissue.
[Para 207] Iodine-125 has Auger electrons as one of its radio-decay byproducts (Figure 2). Auger electrons cause a pronounced biological effect, but have a very short treatment distance. Since NM404 is taken up directly into all cell membranes of cancer cells (including the nuclear membrane), the treatment distance to the DNA is very low. It may effectively allow Auger electrons to be a major contributor to I-125-NM404 treatment effects. [Para 208] The iodine-125 isotope is used for formulation in this application because it exhibits favorable characteristics for cancer radiotherapy, with properties listed below: [Para 209] Gamma Irradiation
(1 ) Radioisotopic half-life: 59.43 days
(2) Gamma energy: 35.5 keV
(3) X-Ray energy: 27.5 - 31.7 keV
(4) Radiation half-distance: 0.02 mm lead; 2 cm in tissue
[Para 210] Auger Electrons
(1 ) Energy of radiation: 1 keV
(2) Number of Auger electrons: Up to 21 per gamma decay
(3) Radiation half-distance: 1 -10 nm [Para 21 1 ] It should be noted that although iodine-1 25 has great utility for in-vivo biodistribution studies and dosimetry extrapolation for diagnostic imaging, it is poorly suited for either whole-body planar imaging or in vivo scintigraphic quantification of tissue concentrations of NM404. However, iodine-124 offers superior characteristics for quantitative determination of in vivo tissue concentrations. Thus, 1 24-I-NM404 will find utility for in vivo pharmacokinetics and biodistribution studies but has no radiotherapeutic effect, lodine-1 31 emits both beta and gamma radiation that produces a therapeutic effect. Although 1-131 -NM404 could potentially be used for radiotherapy, the inventors believe 1-125 to be the more optimal isotope because its lower energy radiation has a shorter radiation haif distance than 1-1 31 and thus hypothesize that it will produce less damage to healthy tissue. Therefore, it was decided that 1-125-NM404 would be used for all clinical radiotherapy studies performed in order to reduce the potential for collateral damage to healthy tissues.
[Para 212] Due to its 60-day physical half-life and low energy 35 keV photon emission, iodine-125 is suitable for imaging experiments in mice and rats. Iodine-125 also provides therapeutic efficacy when used in permanent prostate brachytherapy implants ("brachytherapy seeds"). The major advantage of 1251 is that all the photons are of low energy, insuring very limited exposure of normal tissues surrounding the tumor. The major difference between brachytherapy seeds containing iodine-125 and 1-125- NM404 are the effect of Auger electrons. Since brachytherapy seeds have a metal capsule around the iodine-125, only the low-energy gamma and X-ray are of therapeutic value, and Auger electrons are eliminated by the metal capsule. In difference, I-125-NM404 is taken up into the cancer cell membranes (including into the nuclear membrane), so that Auger electrons can have a major contribution to the therapeutic effect. [Para 213] Although iodine-131 has been used with great efficacy in the treatment of thyroid cancer, a significant disadvantage of iodine-131 is that there is a higher energy gamma emission which could actually expose adjacent surrounding tissues to more radiation than would occur with - iodine-125.
fPara 2141 Dosimetry
[Para 21 5] Dosimetry estimation regarding 1-125-NM404 for an adult female patients were calculated based on SPECT imaging data using female non-tumor bearing rats following administration of 1-1 31 -NM404. The results are listed below:
[Para 216] MIRD extrapolation of 125I-NM404 rat tissue distribution data initially afforded a 5 Rad limiting dose to the adrenals and bladder wall of 2 mCi of 131I-NM4O4. Accordingly the inventors have conducted the preliminary pharmacokinetic studies in human lung cancer patients at 1 mCi.
Similar extrapolative calculation of the dosimetry for 124I-NM4O4 afforded a similar 2 mCi dose level again based on projected dosimetry to the adrenals and bladder wall. Preliminary human data (5 patients), however, indicate very little uptake and retention of the agent into any abdominal organ (including the bladder and adrenals) with the exception of the liver which returns to background levels within 1 1 days. Although these results are not yet complete, it is likely that the actual acceptable 124I-NM4O4 dose will exceed 4 mCi.
i CN
MIRDOSE {IBM PC VERSION 3.1 - AUGUST 1995)
Radiation Dose Estimates for the ADULT FEMALE for 125-I-NM404
Assumptions: Predicted Residence Time from I-131-NM404 female rat biodistribution data
DOSE PRIMARY. SECONDARY
TARGET ORGAN rad/mCi CONTRIBUTOR % CONTRIBUTOR %
D Adrenals .34E-02 2.72E-01 Adrenals 100.0% 0. 0%
Brain .96E-03 2.20E-02 Rem. Body 100.0% 0. 0%
3) Breasts .96E-03 2.20E-02 Rem. Body 100.0% 0. 0%
4) Gallbladder Wall .96E-03 2.20E-02 Rem . Body 100.0% 0. 0%
5) LLI Wall .96E-03 2.20E-02 Rem. Body 100.0% 0. 0%
6) Small Intestine .42E-02 2.75E-01 Small Int. 96.0% Rem. Body 4. 0%
7) Stomach .96E-03 2.20E-02 Rem. Body 100,0% 0. 0%
8) ULI Wall .96E-03 2.20E-02 Rem. Body 100.0% 0. 0%
9) Heart Wall .12E-02 1.15E-01 Heart Wall 100.0% 0. 0%
10) Kidneys .09E-02 1.88E-01 Kidneys 100.0% 0. 0%
11) Liver .66E-02 1.'72E-Ol Liver 100.0% 0. 0%
12) Lungs .43E-02 2.38E-01 Lungs 100.0% 0. 0%
13) Muscle .54E-02 9.40E-02 Muscle 100.0% Uterus 0. 0%
14) Ovaries .10E-02 1.52E-01 Ovaries 100.0% 0. 0%
15) Pancreas .96E-03 2.20E-02 Rem. Body 100.0% 0. 0%
16) Red Marrow .75E-02 1.02E-01 Red Marrow 95.2% Rem. Body 4. 8%
17) Bone Surfaces .84E-O2 6.80E-02 Red Marrow 82.1% Rem. Body 17 .9%
18) Skin .96E-03 2.20E-02 Rem. Body 100..0% 0. 0%
19) Spleen , 79E-02 1.77E-01 Spleen 100.0% 0. 0%
21) Thymus , 96E-O3 2.20E-02 Rem. Body 100.0% 0. 0%
22) Thyroid .98E-01 l.lOE+00 Thyroid 100.0% 0. 0%
2 233)) Urin Bladder Wall , 13E-02 4.18E-02 Urinary Bl 73.7% Rem. Body 26 .3% 24) Uterus , 78E-O2 1.77E-01 Uterus 100.0% 0. 0% 27) Total Body , 55E-02 5.75E-02 Muscle 48.8% Rem . Body 23 .7% 28) EFF DOSE EQUIV .93E-02 1.82E-01 Remainder 35.8% Gonads 20 .8% 29) EFF DOSE .32E-02 1.60E-01 Thyroid 34.5% Gonads 19 .0%
Units of EDE and ED are mSv/MBq or rem/mCi .
RESIDENCE TIMES:
Adrenals 8.99E-02 Ovaries 3.95E-02 hr
Small Intestine 4.68E+00 Red Marrow 3.83E+00 hr
Heart Wall 6.S4E-O1 Spleen 6.29E-01 hr
Kidneys 1.22E+00 Thyroid 4.44E-O1 hr
Liver 5.71E+00 Urinary Bl Cont 2.33E-01 hr
Lungs 4.50E+00 Uterus 3.34E-01 hr
Muscle 3.78E+01 Remainder 1.83E+01 hr
For 5 rads to Adrenals: 18 mCi For 3 rads to Ovaries: 20 mCi For 5 rads to Thyroids: 4 .5 mCi
[Para 21 7] As a result of these dosimetry estimation, the following point have to be considered:
[Para 218] These results were based on a worst-case scenario of no excretion of NM404 from the body. The dosimetry data were calculated from SPECT imaging in rats using I-131 -NM404 and then converted to 1-125- NM404. The adrenal gland seems to be the dose-limiting or critical organ for radiation exposure. In the SPECT imaging experiments the thyroids of rats were unblocked. Thus, the thyroid calculations should be evaluated with caution. fPara 2191 Pharmacology Summary
[Para 220] Comprehensive summary of the synthesis and the biological properties of NM404 is discussed in US Patent Application No. 60/593,1 90 filed on December 20, 2004 U.S. Application No. 10/906,687 filed on March 2, 2005 and U.S. Provisional Application No. 60/521 ,166, filed on March 2, 2004, all of which are incorporated herein by reference for all purposes. [Para 221] The inventors' approach is to design small-molecule carrier molecules which are capable of selectively delivering a diagnostic or therapeutic probe to the desired target tissue capitalizes on unique biochemical or pharmacological properties of molecules displaying a high degree of tissue or tumor selectivity.
[Para 222] It was initially observed that a variety of animal and human tumors contained much higher concentrations of naturally occurring ether lipids in the cell membranes than normal tissue. It was hypothesized that phospholipid ether analogs could accumulate in tumor cells due to their lower capacity to metabolize these lipids. The inventors have pursued radioiodinated phospholipid ether (PLE) analogs as potential tumor-selective imaging agents. Several PLE analogs have exhibited a striking universal ability to selectively localize in a wide variety of transplanted rat, mouse, and human tumor models.
[Para 223] The prevailing hypothesis is that phospholipid ethers become trapped in tumor membranes because of their inability to be metabolized and cleared. Indeed, tumor analysis following administration of radioiodinated phospholipid ethers showed the presence of only the intact agent, whereas analysis of the normal tissues (liver and muscle), urine, and feces revealed only metabolites. Thus, it is the differential clearance rates of phospholipid ether analogs from normal cells versus tumor cells that form the basis of this targeting concept.
[Para 224] Preclinical Studies with First Generation PLE Analogs: Phospholipid ethers can easily be labeled with iodine radioisotopes using radiolabeling methods developed in the labs. The iodophenyl phospholipid ether analogs are specifically designed so that the radioiodine affixed to each molecule is stable to facile in wVø deiodination. It was found that any chemical modification of the phosphocholine moiety or shortening the chain length of the iodophenylalkyl moiety to less than 8 methylenes resulted in little or no tumor uptake. The inventors have now synthesized over 20 radiolabeled PLE compounds and tested in vitro and in vivo. Two of these, namely NM-294 and NM-324 [12-(3-iodophenyl)-dodecyl-phosphocholine], initially showed the most promise in animal tumor localization studies. These prototype compounds, labeled with iodine-125, selectively localized in tumors over time in the following animal tumor models; 1 ) Sprague-Dawley rat-bearing Walker 256 carcinosarcoma; 2) Lewis rat-bearing mammary tumor; 3) Copenhagen rat-bearing Dunning R3327 prostate tumors; 4) Rabbits-bearing Vx2 tumors; and 5) athymic mice-bearing human breast (HT39), small cell lung (NCI-69), colorectal (LSI 74T), ovarian (HTB77IP3), and melanoma tumors. Optimal tumor localization of these agents takes from one to several days due to the more rapid clearance of the radioactivity from normal tissues relative to tumor.
[Para 225] Certain PLE cmpounds discussed in the following paragraphs are shown in Fig. 1.
[Para 226] Clinical Evaluation of NM324: Although first generation compounds NM-324 and NM-294 displayed similar animal tumor localization characteristics, NM-324 was easier to chemically synthesize and was thus selected as the lead compound for initial clinical studies. Although images obtained in several human lung cancer patients detected tumors, images were complicated by high liver radioactivity (Figure 3). [Para 227] Second Generation PLE Analogs: In order to decrease liver uptake and prolong the plasma phase, the inventors examined 9 structural analogs of NM-324 to identify agents that would display improved tumor- to-background tissue ratios with decreased liver uptake. The new PLE analogs were synthesized and radiolabeled with 125I for initial image analysis in Copenhagen rats bearing Dunning R3327 prostate tumors. Based upon this initial screen, NM-404 not only exhibited much lower liver activity than its predecessor NM324 but also maintained prolonged tumor retention (Figure 4. NM404 was therefore selected to undergo further imaging and biodistribution analysis in a variety of animal-tumor models. [Para 228] Accordingly, NM404 has now been evaluated in over 20 xenograft and spontaneous animal tumor models as shown in Table 2. In all tumor models the agent displayed significant tumor uptake and retention regardless of location. Although tumor retention can be explained by a lack of metabolic phospholipase enzymes in the tumor cell membranes, the exact mechanism of tumor cell uptake is unknown. Furthermore, the inventors know the agent does not localize in benign intestinal adenomas (polyps) so it was desirable to further evaluate the propensity of the agent to localize in an intermediate stage of tumorigenesis, namely hyperplasia. The inventors are currently evaluating the selectivity of NM404 in a unique mouse tumor model developed at Wisconsin, wherein both preneoplastic hyperplasias and malignant adenocarcinomas form spontaneously in the mammary gland. Preliminary results in this model indicate that the agent is not taken up and retained in preneoplastic lesions, and thus, appears to be exclusively retained by malignant tumor cells. If this initial observation is validated, then via genomic and or proteomic analysis the inventors may be able to identify a key genetic difference between malignant tumors and their non malignant predecessor cells. This could identify a completely new molecular therapeutic target that may be universal for all types of cancer. [Para 229] The table below summarizes the wide variety of cancer types and tumor lines that have been evaluated regarding accumulation by NM404. [Para 230] Table 2: Tumor models examined with NM404
NM 404
Tumor Model Species T ype Tumor Localization*
Human Tumor Xenografts
Prostate P C -3 SCID Mouse Adenocarcinoma Yes Lunq A-549 CNSCC) SCID Mouse Adenocarcinoma Yes
Lung NCI H-69 (Oat Cell) Nude Mouse Adenocarcinoma Yes Adrenal H -295 SCID Mouse Adenocarcinoma Yes Adrenal R L-251 SCID Mouse Adenocarcinoma Yes
Melanoma A-375 Nude Mouse Adenocarcinoma Yes Colon LS-180 Nude M ouse Adenocarcinoma Yes
Ovarian HTB -77 Nude M ouse Adenocarcinoma Yes
Artmtaf Tumor Xenografts
Mammary MCF-7 Rat Adenocarcinoma Yes Prostate MatLyLu Rat Adenocarcinoma Yes
Walker-256 Rat Carcinosarcoma Yes Recent Rodent Tumor Mbdeis
[Para 231] Tissue Distribution and Kinetics
[Para 232] Consistently, NM404 has been found to be retained in tumor tissue for long and extended periods of time. Tumor concentrations are almost stable for many weeks following administration of NM404, showing slow elimination from cancerous tissue over time. In contrast, NM404 is eliminated from normal tissue within a few days reaching very low levels. [Para 233] Additionally, NM404 was designed to have a long blood half life. This ensures prolonged exposure of NM404 to the tumor tissue and ensures uptake of up to 10-25% of the injected dose into the tumor tissue. The inventors believe that it is very important for a radiotherapy compound to have a large portion of the injected dose accumulating in the tissue of interest. As a consequence of the long blood half life, NM404 will continuously accumulate in tumor tissue over time. An example of this pattern is provided in Figure 5. This may lead to a delayed onset of the therapeutic effect until the accumulation of NM404 into the tumor tissue has been continuing for several days or weeks. [Para 234] Blood Plasma Kinetics
[Para 235] The first-generation prototype compound NM324 was found to have a elimination half life time in plasma of 2.43 hours in rats. By comparison, the lead compound NM404, has a elimination half life time of roughly 209 hours in rats (distribution phase half life is 4.86 hours).
fPara 2361 Radiotherapeutic Study of 1-125-NM404
[Para 237] During the course of mouse tumor uptake and retention studies with "imaging" doses (15-20 μCi/20g mouse) of 125l-labeled NM404, several apparent therapeutic responses have been observed (unpublished results). In an ApcMi'nl-v mouse mammary tumor model it has generally been noted that tumor growth remains static following a single intravenous injection of NM404. Some of these animals also lost all hair above larger mammary tumors at around 8 days after injection. Moreover, these mice also get intestinal tumors and usually suffer from intestinal bleeding resulting in severe anemia, which renders their feet white. Dr. Moser noted that the feet of these mice had reverted to a pink color around 5 days after a single injection of NM404. Upon eventual dissection of these animals, it was noted that only a very few, if any, of the expected 20 or so intestinal tumors usually found at this age actually remained. The "white to pink feet" phenomenon was also observed in a separate, but more aggressive, mouse intestinal adenocarcinoma model, wherein dissection at 12 days following NM404 administration, again revealed that most, if not all, of the expected intestinal tumors were gone. After 21 days In both intestinal models, animals that received NM404 easily outlived their untreated litter mates. Another compelling example of tumor regression is illustrated in Figure 6. Two litter mates each received SCCl and SCC6 xenografts in their left and right flanks, respectively. One mouse received a single injection of 125I-NM4O4 (20 μCi). The mouse that didn't receive NM404 died 21 days later, whereas the tumors in the treated mouse regressed significantly and the animal was quite healthy 80 days after injection. These coincidental findings were reconfirmed in two separate age-matched groups each involving more than 6 mice. Although these observations with 125I-NM4O4 are anecdotal at this point, they do seem to strongly indicate potential for radiotherapy applications particularly if labeled with iodine-131 . Ongoing quantitative tumor uptake and retention studies in several animal tumor models will also provide sufficient data to initiate a comprehensive dosimetry analysis for this agent in order to estimate its true radiotherapeutic potential.
[Para 238] Due to its 60-day physical half-life and low energy 35 KeV photon emission, iodine-125 is suitable for imaging experiments in mice and rats. Iodine-125 also affords therapeutic characteristics. In one imaging experiment (Figure 6), 2 nude mice were each inoculated with subcutaneous squamous cell lines SCCl and SCC6 tumor cell implants on opposing flanks. SCCI and SSC6 cells were used because one is radiosensitive relative to the other. After 14 days when the average tumor size was approaching 0.5 cm in diameter, one of the mice received 20 μCi of I-125-NM-404 and the other one receive unlabeled NM404 in an equal mass dose. The mouse receiving the unlabeled cold compound had to be euthanized 20 days after injection due to both tumors reaching the termination size limit as defined in the animal use protocol. Both tumors in the 125I-NM4O4 mouse regressed dramatically and unexpectedly over the course of several weeks (Figure 6) after a single injection of an imaging dose of NM404. This mouse never did reach terminal tumor size and the mouse was actually euthanized after 90 days in order to collect histology sections.
[Para 2391 Preliminary Report of Ongoing Tumor Therapy Studies
[Para 240] Preclinical study-preliminary results:
TPara 2411 Efficacy of single injection of 1-125 labeled SF404A in an A549
SCID mouse model
[Para 242] Purpose and rationale: To determine an effective dose for radiotherapy using single injection of 1-125 labeled SF404A in a xenograft tumor model fPara 2431 Tumor model:
[Para 244] A549 (human non-small cell lung cancer, NSCLC, from ATCC) are maintained in Ham's F-12K media supplemented with 10% fetal bovine serum. Tumor cell suspension (1 xl O6 cells in phosphate-buffered saline) is injected s.c. into the right flank of female SCID mice (6-8 weeks, CB-
1 7 ficxHsό-Prkcd scld, Harlan). Animals are given free access to food and water, tumor growth and animal weight are monitored. Upon reaching 4-
5mm in diameter, mice will be divided into groups of 6 for therapy study. fPara 2451 Dosing:
[Para 246] Single injection of SF404A at Day 0 fPara 2471 Croups: (1) 5 study groups: 4 treatment + 1 control
(2) N=6 per group
(3) 4 acute dose levels: 50, 1 50, 250 and 500 μCi per mouse
(4) Control group dosed with equivalent mass amount of NM404:
(5) Control: 0 μCi per mouse ("cold" NM404)
[Para 2481 Efficacy Assessments:
(1) Tumor size (caliper) measured once a week.
(2) Survival
(3) General appearance (activity, alertness, mobility)
(4) Until 10 weeks post-injection or until no animal in the control group is alive, whatever comes first
(5) Histopathology assessment of tumor or residual tumor site. [Para 249] Photograph of tumor at Day 0, 30, and 60 for each animal. [Para 250] The purpose of the study was to determine an effective dose for radiotherapy using single injection of 1-125-NM404 (formulation SF404A) in a xenograft tumor model.
[Para 251 ] The tumor model used for this study was A549, a human non- small cell lung cancer, (NSCLC) obtained from ATCC (Manassas, VA). The tumor cells are maintained in Ham's F-I 2K media supplemented with 10% fetal bovine serum. Tumor cell suspension (1 x106 cells in phosphate- buffered saline) is injected s.c. into the right flank of female SCID mice (6-8 weeks, CB-I 7 /IcrHsd- Prkcd scld, Harlan). Animals are given free access to food and water. Upon reaching 5-10 mm in tumor diameter, mice will be enrolled in the study.
[Para 252] A single intravenous injection of SF404A was performed at Day 0. For the control group, cold compound (C-NM404) has been injected. For treatment groups I-125-NM404 has been injected.
[Para 253] The study contained 5 groups; 4 treatment groups (n=6/group) and 1 control group (n=9). The iodine doses for the treatment groups were 50, 1 50, 250 and 500 μCi per mouse. The control group was dosed with an equivalent mass amount of C-NM404 (0 μCi). [Para 254] For assessment of efficacy of treatments, the following assessments were performed:
(1 ) Tumor size (caliper) measured once a week
(2) Survival
(3) General appearance (activity, alertness, mobility)
(4) Digital pictures of tumor-bearing mouse [Para 255) Assessments were made until 10 weeks post-injection or until no animal in the control group is alive, whatever comes first. Histopathology assessment of tumor or residual tumor site was performed at the end of the study.
(1 ) 50 μCi group: 6 animals were enrolled;
(2) 1 50 μCi group: 5 animals were enrolled;
(3) 250 μCi group: 6 animals were enrolled;
(4) 500 μCi group: 6 animals were enrolled.
[Para 256] As part of this preliminary report, the inventors include the following animals in the analysis:
(υ Control: n=7
(2) 50 μCi group: n=4
(3) 1 50 μCi group: n=5
(4) 250 μCi group: n=6
(5) 500 μCi group: n=5
[Para 257 The baseline tumor volumes (in mm3) for each rou were
[Para 258] Since the tumor volumes of the control group and the 1 50 μCi group was substantially larger than the other groups and from what was previously planned, a complete re-enrollment of both groups with smaller tumor sizes has been initiated. As far as the tumor volumes for the 500 μCi group, the inventors believe that the study may be biased against this group. Because of necrosis and inefficient blood supply,, larger tumors may not respond as well to I-125-NM404 treatment.
[Para 259] The following average tumor volume for each group was recorded over the 10-week assessment eriod:
[Para 260] This is also reflected in the Fig. 10
[Para 261 ] In summary, control animals show rapidly growing tumors over the 10-week assessment period. This confirms that the compound itself C- NM404 has no substantial effect on tumors growth. The 50 μCi dose group did not show any difference to control animals, hence this seems to be an ineffective dose in this animal model. The 1 50 μCi dose group shows no treatment effect as well, however (as was pointed out before) this dose group started with unusually large tumors at Day 0, however this may show different results with this dose group of 1 50 μCi with smaller tumors. [Para 262] Preliminary data indicates that both the 250 and 500 μCi show a substantial and prolonged treatment effect. Tumor volumes are stable and same tumors appear "collapsed" (the tumor surface has caved in, as shown in Fig. 7), Additionally, hair above the tumors fells off confirming substantial accumulation of radioactivity in these tumors.
fPara 2631 Other Ongoing Preclinical Tumor Therapy Studies fPara 2641 Efficacy of fractionated dose vs single injection of 1-125 labeled
SF404A in an A549 SGD mouse model
[Para 265] This preclinical study assesses fractionating the 1 50 μCi dose by injecting 3 weekly 50 μCi doses instead of one single 150 μCi dose as given in the previous study.
[Para 266] Purpose and rationale: To determine the radiotherapeutic efficacy of a fractionated dose of 1-125 labeled SF404A vs a single injection of an equivalent total dose in a xenograft tumor model This study is an adjunct to CLTR-Pre-05-001 , which contains the 1 50 μCi single dose used for comparison. fPara 2671 Tumor model:
[Para 268] A549 (human non-small cell lung cancer, NSCLC, from ATCC) are maintained in Ham's F-] 2K media supplemented with 10% fetal bovine serum. Tumor cell suspension (1 x106 cells in phosphate-buffered saline) is injected s.c. into the right flank of female SCID mice (6-8 weeks, CB-
17/\crHsd-Prkcdsc/cf, Harlan). Animals are given free access to food and water, tumor growth and animal weight are monitored. Upon reaching A-
5mm in diameter, mice will be divided into groups of 6 for therapy study.
[Para 2691 Dosing:
[Para 270] Single injection of 1-125-SF404A at Day 0 vs a fractionated dose of I-125-S404A
FPara 2711 Groups:
[Para 272] 2 study groups: 1 Fractionated dose, 1 Single injection
(1 ) N=6 per group
(2) 1 50 μCi (3 x 50 μCi) per mouse by by fractionated injection, at 1 week intervals from day 0
(3) 1 50 μCi per mouse by single injection TPara 2731 Efficacy Assessments:
(1 ) Tumor size (caliper) measured once a week.
(2) Survival until 10 weeks post-injection or until no animal in either group is alive, whatever comes first
(3) General appearance (activity, alertness, mobility)
(4) Histopathology assessment of tumor or residual tumor site.
(5) Photograph of tumor at Day 0, 30, and 60 for each animal.
[Para 2741 Efficacy of single injection of 1-125 labeled SF404A in a PC-3
(Prostate cancer) SCID mouse model
[Para 275] Purpose and rationale: To determine an effective dose for radiotherapy using single injection of 1-125 labeled SF404A in a prostate cancer xenograft tumor model
FPara 2761 Tumor model:
[Para 277] PC-3 (human prostate cancer, from ATCC) are maintained in
Ham's F-12K media supplemented with 10% fetal bovine serum. Tumor cell suspension (I xI O6 cells in phosphate-buffered saline) is injected s.c. into the right flank of male SCID mice (6-8 weeks, CB-I 7 /\crHsd- Prkcdsc/tf, Harlan),
Animals are given free access to food and water, tumor growth and animal weight are monitored. Upon reaching 4-5mm in diameter, mice will be divided into groups of 6 for therapy study. fPara 2781 Dosing:
[Para 279] Single injection of SF404A at Day 0
[Para 2801 Groups:
(1 ) 5 study groups: 4 treatment + 1 control
(2) N=6 per group
(3) 4 acute dose levels: 50, 1 50, 250 and 500 μCi per mouse
(4) Control group dosed with equivalent mass amount of NM404; 0 μCi per mouse ("cold" NM404)
TPara 2811 Efficacy Assessments:
[Para 282] Tumor size (caliper) measured once a week.
[Para 283] Survival: until 10 weeks post-injection or until no animal in the control group is alive, whatever comes first
(1 ) General appearance (activity, alertness, mobility)
(2) Histopathology assessment of tumor or residual tumor site.
(3) Photograph of tumor at Day 0, 30, and 60 for each animal. [Para 284] This preclinical study duplicates the previous study single injection study but with a different tumors model.
fPara 2851 Clinical evaluation for imaging of prostate and NSCLC using a phospholipid ether analog. NM-404.
[Para 286] Safety assessment in normal male subjects healthy normal female subjects were carried out and it was determined that a dose level of 3 μg/kg of C-NM-404 is safe to administer to male subjects. This dose is 10 fold the 0.3 μg/kg dose of 131I-NM4O4 anticipated to be used for imaging in patients with metastatic prostate cancer. A dose level of 3 μg/kg of C-NM- 404 is safe to administer to female subjects. This dose is 10 fold the 0.3 μg/kg dose of 131I-NM4O4 anticipated to be used for imaging in patients with metastatic lung cancer. fPara 2871 Imaging Characteristics of NM-404 in Patients with NSCLC [Para 288] Metastatic Non-Small Cell Lung Cancer Subjects: [Para 289] Written informed consent will be obtained. 131I-NM4O4 at a dose of 0.3μg/kg will be infused over 10 minutes. The aqueous solution containing 131I-NM4O4 will be prepared using sterile technique in Dr. Jamey Weichert's laboratory at the University of Wisconsin. The preparation dispensed by the Nuclear Pharmacy will be certified as sterile and pyrogen- free. The subject will be monitored for adverse reactions during and after the infusion. Vital signs will be checked immediately after the infusion, and at 60 minute intervals up to four hours post-injection. They will be observed for adverse events throughout this time. The subjects will return 24, 48, 72, 96, 120, 144 hours and 30 days after the infusion. NM404 Pet scans will be obtained at 4, 8, 24, 48 and 96 hours post injection. Vital signs will be monitored at these times. Any adverse reactions experienced by the subjects will be recorded. All subjects will receive SSKI oral solution beginning one day prior to the 1311-NM404 infusion and continue for 7 days to reduce thyroid exposure to free radioiodine. Serum pharmacokinetics will be drawn pre-infusion, 5, 10, 30, 60, 120, 240, 360 minutes post-injection and at 24, 48, 72 and 96 hours post injection. Sequential 24 hour urine collections for 0-24, 24-48, 48-72 and 72-96 hours post injection will be obtained. [Para 290] Plasma pharmacokinetics confirmed an average 1 1 3.1 hours (SEM = 7.9 h) elimination half life time for NM404.
[Para 291 ] An average of 3.4% (range from 0.9% to 9.8%) of injected dose was eliminated via the kidneys with 96 hours of intravenous administration of NM404. A dose level of 0.3 μg/kg of 1311-NM404 was safe to administer to subjects with advanced non-small cell lung cancer. The elimination plasma half life time of NM404 was found to be 1 13.1 hours and the urinary elimination was found to be roughly 3.4% within 96 hours post-injection.
fPara 2921 Prostate Cancer
The present invention provides preliminary data regarding the use of the second-generation PLE analog, NM404, in imaging patients with prostate cancer. This agent, currently under investigation at the University of Wisconsin, is selectively retained in tumors in high levels, and has high sensitivity and specificity in preclinical models. It has passed acute toxicology testing in both rats and rabbits at >1000 times the anticipated human imaging dose, and the unlabeled agent was administered at 10 times the anticipated imaging mass dose to 10 normal volunteers at the University of Michigan and University of Wisconsin to document safety. The inventors hypothesize that imaging with NM404 will ultimately prove as sensitive as imaging with FDC, will also be more specific, may afford therapeutically utility and due to its relatively long half-life be available in virtually every PET facility regardless of location.
[Para 293] The biodistribution, kinetics, optimal imaging times, and dosimetry of 131I -NM404 are currently being evaluated in a pilot study in patients with lung cancer (NSCLC) at UWCCC. This agent has also been evaluated preclinical^ in a metastatic prostate tumor model in which lymph node metastases were clearly delineated by scintigraphy following intravenous administration of NM404 but, pertinently, the tracer was not retained by uninvolved lymph nodes. Selectivity of NM404 for malignant cells is particularly relevant in prostate cancer as conventional tumor markers such as PSA may also be elevated in a variety of benign disease states including prostatitis and benign prostatic hypertrophy. Furthermore, selective uptake and prolonged retention by tumor cells supports a role for NM404 as a tumor-selective diagnostic and therapeutic agent. [Para 294] The preliminary results will provide the preliminary data for a subsequent study designed to more accurately estimate the predictive power of NM404 for staging and/or following response to therapy in prostate cancer. In addition since NM404 has high tumor uptake, this agent also has the potential to be developed as a therapeutic agent when coupled with higher doses of 131I1 125I, another halogen astatine, lodine-125 would be especially desirable in prostate cancer patients due to its short Auger electron path length in tissue, which would theoretically minimize radiation effects in neighboring normal tissues like the rectum. In a therapeutic sense, the 60 day half-life of iodine- 125 matches exceedingly well with the prolonged tumor retention properties of NM404.
[Para 295] Targeting Tumor Cells
[Para 296] One approach to the development of sensitive, more available imaging exams is to design carrier molecules which are capable of selectively delivering a radiopharmaceutical probe to the desired target tissue. The approach has been to capitalize on the unique biochemical and pharmacological properties of phospholipid ether analogues such as NM404, which displays a high degree of tumor selectivity. Phospholipids are an essential component of cellular membranes where they impart structural integrity and are heavily associated with a variety of cell signaling processes. Phosphatidylcholine, commonly known as lecithin, is such an example. Phospholipid ethers, on the other hand, represent a minor subclass of phospholipids that also reside in membranes. As the name implies, these lipids contain an ether rather than an ester linkage at the C-I position. Platelet-activating factor (PAF) represents one of the better known phospholipid ethers. As described above, tumors retain PLE is due to the differential clearance rates of PLE from normal cells versus tumor. [Para 297] While initiating human pharmacokinetic studies with the prototype agent, NM324, ongoing experiments to identify PLE compounds with superior tumor localization and background clearance properties were performed. Based upon this work, NM404 [12-(4-iodophenyl)- octadecylphosphocholine] was selected due to its enhanced ability to localize in tumor, its increased metabolic clearance from the liver, and its longer plasma half-life. In a key experiment documenting the ability of NM404 to localize in metastases, lymph node metastases were clearly delineated by scintigraphy in a metastatic prostate tumor model following intravenous administration of NM404, but the tracer was not retained by uninvolved lymph nodes.
[Para 298] Comparative scintigraphic imaging results for NM324 and NM404 in PC-3 prostate tumor-bearing SCID mice revealed high tumor-to- normal tissue ratios and significant decreases in background abdominal and liver radioactivity with NM404 (Fig 19). This agent has now been evaluated in 27 animal tumor models and it is clear that once the agent enters tumor cells, it reaches a metabolic dead end and becomes trapped. Prolonged tumor retention of this agent is demonstrated in a human adrenal tumor xenograft implanted into SCID mice (Fig 20). Using izsi-labeled NM404, the inventors have been able to image mammary and prostate tumors in mice in excess of 60 days. Prolonged tumor retention characteristics significantly enhance the radiotherapeutic efficacy of the agent.
[Para 299] Recent imaging and biodistribution studies performed in rodent models aimed at determining the uptake characteristics in a wide variety of xenograft and spontaneous (endogenous or transgenic) tumor types are summarized above. These agents have displayed selective localization and prolonged retention in every malignant tumor regardless of anatomic location (including lymph nodes) studied to date.
[Para 300] Preliminary Scintigraphic Imaging Results with NM404 in Prostate Tumor-Bearing Mice: In a preliminary experiment to show that NM404 localizes in mouse prostate tumors, TRAMP mice were scanned on a Bioscan AR-2000 radioTLC scanner (modified in the lab for mouse imaging) from 1 -8 days after tail vein injection of 125I-NM4O4 (1 5 μCi). Following in vivo imaging of anesthetized mice, the prostate tumors were removed immediately, and imaged ex vivo on the same scanner (equipped with high resolution 1 mm collimator and 2-D acquisition and analysis software) in order to avoid tissue attenuation associated with the low energy of iodine- 125 (Fig 21 ). Although the number of prostate tumor-bearing animals was small (n=4), preliminary images and tumor-to-background data indicated uptake of NM404 into prostate tumor but not benign hyperplasia found in this model. In an attempt to simulate bone metastases another experiment was performed wherein human PC-3 tumor cells were implanted into the tibia of immune compromised NUDE mice (n=6) according to a recent reports. Following tumor cell inoculation, mice were scanned serially by high-resolution microCT in order to monitor bone tumor development (Fig 22). Once bone deterioration was detected, iodine-125 labeled NM404 was administered intravenously via tail vein. Mice were scanned for radioactivity and by microCT 4 days post NM404 administration. Radioactivity scans were fused with microCT scans (Fig 23) to corroborate NM404 radioactivity and tumor location.
[Para 301 ] Biodistήbution Data Extensive biodistribution data for the prototype agent 125I-NM324 in several tumor models has previously been reported. Tumor-to-blood ratios exceeding 8:1 were seen at delayed times post-injection. For example, in a rat mammary tumor model, tumor-to- normal tissue ratios reached a maximum at 96 hours with a tumor-to-blood ratio of 8.6 and tumor-to-muscle ratio of 20:1. Moreover, the biodistribution of PLE-associated radioactivity is heterogeneous in tumor, as demonstrated by microautoradiogram studies showing that the PLE radioactivity resides exclusively in viable tumor cells located toward the outer regions rather than the central necrotic regions. Comparative biodistribution data for NM324 and NM404 in SCID mice have been obtained in prostate and A549 lung cancer tumor models. These studies revealed high tumor-to- normal tissue ratios and tumor uptake exceeding 25% of the injected dose with NM404, thus supporting the desire to examine the biodistribution of PLE analogs in humans.
[Para 302] Isotope Selection Because the tumor-targeting strategy of PLE analogs appears to involve selective tumor retention over time, relatively short-lived nuclides such as '8F or even 99mTc are not practical for labeling NM404 at the current time. With a 13h half-life and optimal imaging characteristics, iodine-1 23 may also prove suitable for this agent when scanned in a 3-dimensional SPECT mode. Imaging with iodine-1 23 will require further investigation. While tumor localization of PLE analogs appears to occur within several hours of injection (NM324 images obtained 6h post injection in a lung cancer patient showed intense lung tumor uptake in a prior study), planar 2-dimensional imaging, like that currently being performed with iodine-1 31 in humans, requires a delay period to allow background activity to clear from neighboring normal tissues and blood. It is likely that earlier imaging may be possible when scanning with PET and 3D SPECT where neighboring radioactivity is less interfering due to the 3- dimensional nature of these modalities. In organs where background radioactivity remains inherently low (brain for example), it may be possible to use gamma emitting isotopes like iodine-123 which in addition to providing beautiful images, would permit image acquisition later on the same day of injection. Although the use of other isotopes may ultimately prove suitable for use with NM404, the current focus will develop the PET imaging capability of this agent due to the recent success of oncologic imaging using hybrid PET-CT scanners. The unsurpassed diagnostic accuracy afforded by a biochemical or functional tumor imaging PET agent combined with the precise anatomic accuracy provided by CT is now the gold standard for tumor imaging. However, it is highly advantageous to label PLE analogs with iodine-124, a relatively new PET isotope, wherein the physical half-life (4 days) matches well with PLE tumor uptake and retention kinetics. Labeling NM404 with iodine-124 represents a natural extension of prior studies with gamma-emitting nuclides. It has been shown that PET imaging with 124I affords over 40 times the sensitivity of planar 131l-gamma scintigraphy. PET, unlike traditional gamma camera imaging, also offers significant resolution enhancement, image quantification, and 3-dimensional capabilities. Due to the preliminary success of 131I-NM4O4 in the current lung cancer imaging trial, it is now imperative to label NM404 with iodine-1 24 and evaluate its tumor detection efficacy by PET in order to overcome the limitations inherently associated with planar scintigraphy
[Para 303] The utility of tumor tracers like 67Ca-citrate and '8F-FDG is limited by their lack of specificity to distinguish neoplasm from inflammation. This lack of specificity is a significant clinical issue in patients with cancer. However, preliminary studies with PLE analogs offers promise in overcoming this limitation. Prior experiments conducted in rats revealed no uptake and retention of NM324 into carrageenan-induced granulomas. Gallium citrate, however, utilized as a control in this study, did indeed concentrate significantly in the granulomatous lesions. Thus, this preliminary finding that PLE analogs don't apparently localize in inflammatory lesions further justifies the need for evaluation of this agent in human cancer patients. Although FDG-PET has paved the way for hybrid imaging, its lack of tumor cell specificity will always limit its diagnostic efficacy. New molecularly targeted agents, like NM404, which display universal tumor uptake and selective retention regardless of location, as well as selectivity for malignant tumor cells and not inflammatory or hyperplastic lesions, will represent a significant improvement in the detection and characterization of cancer.
[Para 304] While planar nuclear medicine imaging techniques have historically afforded acceptable 2D images, this modality offers no tomographic capability and poor image quantitation. Although 125I-NM4O4 was suitable for preliminary scintigraphic imaging and tissue distribution studies in rodent tumor models and '31I was suitable for Phase 1 safety and pharmacokinetic evaluation in human lung cancer patients, neither are optimal for quantitative in vivo human imaging. PET imaging with iodine- 1 24, a relatively new and commercially available positron isotope with a 4-day half-life, would alleviate many of the problems associated with planar imaging. Transitioning the unique tumor imaging capabilities of NM404 into PET scanning has now become a major goal of our lab since the iodine-124 became commercially available late last year. If the tumor specificity of NM404 for malignant tumors seen in mouse models is confirmed in humans, then we would have an agent with more tumor selectivity than FDG, albeit without its inflammatory site localization properties. Moreover, the agent could be manufactured in one facility and shipped to virtually any location in the world due to its 4-day half life.
[Para 305] The inventors recently successfully radiolabeled NM404 twice with iodine-124 from a commercial vendor (Eastern Isotopes). The radiochemical yield (>60% mean isolated yield, >99% purity) was very similar to what we usually obtain with commercial sources of iodine-125 or 131 sodium iodide. The PET imaging characteristics of 124I-NM4O4 in a rat CNS-I brain tumor model (Fig 24) was investigated. Imaging times in this preliminary study were limited to 24 h and 4 days post injection due to microPET scanner availability. MicroPET images acquired 24h after iv injection of 124I-NM4O4 were corroborated with contrast-enhanced MRI images and showed intense uptake of the tracer in the brain tumor accompanied by little or no uptake in surrounding intact brain tissue. This study represents the first PET image obtained with NM404 and demonstrates the ability to efficiently radiolabel, purify, and formulate NM404 for PET imaging. These compounds may then be used for extending NM404 PET utility into human cancer patients.
fPara 3061 Determine tumor uptake and retention characteristics of 124I NM404 by PET-CT in patients with radioαraphically evident metastatic prostate cancer.
[Para 307] Objectives and Rationale
[Para 308] Assessment of whether selective detection of known metastatic lesions by radioiodinated NM404 is possible and comparable to conventional radiologic modalities is discussed in the following paragraphs. Inclusion criteria for this study will consist of fifteen patients with metastatic prostate cancer, with at least 5 patients having soft-tissue prostate cancer metastases and at least 5 of the patients with bony metastases identifiable by conventional radiologic studies which include CT scan and bone scan. Following patient enrollment, uptake of radiolabeled NM404 will be measured by 1-1 24 isotope PET-CT scan and correlated with the radiographically evident lesions detected by the patient's other conventional staging studies.
[Para 309] Methods
[Para 310] Synthesis, Radfolabefing, and Formulation: Radioiodination of stable NM404 with 124l-sodium iodide is routinely achieved by modification of an ammonium sulfate-mediated isotope exchange reaction reported by Mangner and recently optimized for NM404 in our lab. Exchange reaction methodology has been used effectively for initial human trials with NM324, the predecessor of NM404 and is currently being used for preclinical studies and the human lung cancer trial. Briefly, a 2-ml glass vial is charged with 10 mg of ammonium sulfate dissolved in 50 μl of deionized water. Fourteen 2mm chemical resistant glass beads are added, a Teflon lined septum and screw cap are added and the vial gently swirled. A solution of 20 μg (in 20 μl of ethanol) of stock NM404 is added followed by aqueous sodium iodide (124, 1 -5 mCi) in less than 30 μl aqueous 0.01 N sodium hydroxide. The isotope syringe is rinsed with three 20-μl portions of ethanol. The reaction vile is swirled gently. A 5-ml disposable syringe containing glass wool in tandem with another 5-ml charcoal nugget filled syringe with needle outlet are attached. The glass wool syringe acts as a condensation chamber to catch evaporating solvents and the charcoal syringe traps free iodide/iodine. The reaction vessel is heated in a heating block apparatus for 45 minutes at 1 50° C. Four 20 ml volumes of air are injected into the reaction vial with a 25-ml disposable syringe and allowed to vent through the dual trap attachment. The temperature is raised to 160° C and the reaction vial is heated another 30 minutes. After cooling to room temperature, ethanol (200 μl) is added and the vial swirled. The ethanolic solution is passed through a pre-equilibrated Amberlite IRA 400-OH resin column to remove unreacted iodide. The eluent volume is reduced to 50 μl via a nitrogen stream (use charcoal syringe trap) and the remaining volume injected onto an HPLC silica gel column (Perkin Elmer, 3 μm X 3cm disposable cartridge column eluted at I mI/ min with hexane/isopropanol/water (52:40:8)) for purification. Final purity is determined by TLC (plastic backed silica gel-60 eluted with chloroform-methanol-water (65:35:4, Rf =0.1 ). The HPLC solvents are removed by rotary evaporation and the resulting radioiodinated NM404 is solubilized in aqueous 2% pharmaceutical grade Polysorbate-20 (0.1 μl/mg of compound). The ethanol is removed under vacuum and the residue dissolved in sterile water to give a final solution containing no more than 2- 3% Polysorbate-20. Sterilization will be achieved by filtration through a sterile 0.2 μm filter unit. Each of the solutions will be tested for pyrogens using the Limulus Amebocyte Lysate test kit. This is the same procedure currently employed for the preparation, purification and sterile formulation of 1-131 -labeled NM404 for lung cancer patient studies. The drug Master Formulation Card and Product Preparation Checklist are included in the supplemental section of this proposal. We have performed this radioiodination hundreds of times with either iodine-1 31 or 125 and typically achieve radiochemical yields ranging from 60-90% (isolated pure product) with specific activities of exceeding 130 mCi/μmol of NM404. This method has afforded sufficient pure injectable 131I-NM4O4 for our ongoing preclinical and human trials. We have not had a labeling failure to date. Moreover, and relevant to the current proposal, we have recently successfully radiolabeled NM404 with iodine- 124 sodium iodide (5 mCi) from Eastern Isotopes in >60% isolated radiochemical yield. Although acceptance criteria require >95% radiochemical purity, results typically exceed 99% since the entire reaction mixture is subjected to preparative HPLC purification. [Para 31 1 ] 1-124 Dose for Human Administration: The toxicity profile, biodistribution, kinetics, optimal imaging times, and dosimetry of 131I NM404 are currently being evaluated in an ongoing study in patients with lung cancer (NSCLC) at UWCCC, as discussed above. Dosing of radioiodinated 124I NM404 will be based on MIRD extrapolation of the iodine-131 dosimetry data derived from this ongoing study. The 131I-NM4O4 dose is currently calculated as follows: Animal biodistribution data is used to determine the percentage of injected dose/organ at varying time points. These animal data are extrapolated to man by means of MIRD formalism (MIRDOSE PC v3.1) using standard conversion factors for differences in organ mass and anatomy between rat and standard man, providing predicted human organ doses; Based on these predicted doses, the permissible mCi dose to be injected into humans is determined using the maximal doses legally permitted by RDRC regulations for specific human tissue as defined in the Federal Register (21 CFR Part 361.1) e.g. whole body, blood, blood forming tissues, eye lens, gonads - 3 rem/dose; any other tissue - 5 rem/dose. This approach was previously used to bring both NM324 and NM404 to clinical use, resulting in a calculated dose of 1 mCi. New animal data acquired with NM404 (5 rad testes dose=2.1 mCi and 5 rad dose to adrenals=2.2mCi) indicate the starting maximum dose of 131I -NM404 will be around 1.9 mCi or twice that of NM324.
[Para 312] Study Procedures: Before infusion, an intravenous line will be established in the arm. The tracer dose <0.3 μg/kg body weight 12^l-NM404 (1 mCi, actual dose pending current study dosimetry results) will be infused over 5 minutes. The preparation will be sterile, pyrogen-free, and contain <5% free iodine by thin layer chromatography (usual result is <1%). The patient will be clinically monitored for adverse reactions during the infusion. [Para 313] Since the animal toxicity studies (using over 1 ,000 times the maximum planned quantity of 124I-NM4O4 in this study) and the preliminary clinical safety studies with the unlabeled compound (at doses 10 times greater) were without side effects, we do not anticipate toxicity at the imaging dose. However, we are cognizant of the possibility of untoward allergic or other reactions. The patients' vital signs (pulse, BP, temperature and respiratory rate) will be re-checked immediately after the infusion and at 30 and 60 minutes post injection. The vital signs will then be checked each hour for two hours.
[Para 314] Baseline PET-CT images (whole body and selected regional conjugate views) for the first five patients will be obtained at 48 and 96' hours post injection. PET images will be obtained using a CE Clinical CE Discovery LS PET-CT scanner utilizing appropriate attenuation correction and optimized to iodine-124. The 48 and 96 hour time points are based on preliminary data regarding optimal imaging times for 131I-NM4O4 in lung cancer, however, the optimum tumor-targeting time for 124I-NM4O4 in human prostate cancer is yet to be determined.
[Para 31 5] 124I-NM4O4 tracer accumulation and washout will be recorded on the GE Discovery LS PET/CT scanner or on CE Advance PET scanner available at our clinic. A CT scan will be performed first for patient localization and attenuation correction on the Discovery LS PET/CT scanner; a transmission scan will be performed on the Advance PET scanner. Each PET scan will be corrected for random, dead time, attenuation, and scatter. First scanning will be performed immediately at 48 and 96 h after the 124I-NM4O4 injection. Whole body scans will be performed with increased scanning time over the tumor site (up to 30 min). Images will be reconstructed using an iterative OSEM reconstruction method with attenuation correction, and smoothed with a Gaussian filter.
[Para 316] Data evaluation will be based on a region-of-interest (ROI) analysis of coregistered PET images. CTs from each PET/CT scan will be co- registered to enable accurate positioning of the PET data, ROIs will be outlined to represent various organs in the field of view (typically heart, lung, muscle, liver, stomach, spleen, intestine, kidney, and bladder). For each ROI and for each time frame, the average radioactivity concentration will be calculated and standardized uptake values (SUVs) calculated. Detailed pharmacokinetic analysis will not be performed in this study, but will have been completed in the ongoing lung cancer trial and not duplicated here, because this will be performed in the lung cancer trial, wherein 2 patients will undergo PET scanning immediately after, and subsequently at 6, 24, and 48 h post NM404 infusion. SUVs will be plotted as time-activity curves. The time course of radioactivity in each ROI will be fitted using various kinetic models including a two-rate constant in a two compartment model and a four-rate and three-rate constant, in a three compartment model in conjunction with the non-linear regression fitting. The nonlinear least- square fitting will be done using a modified gradient-expansion algorithm. The best fits will be determined by minimizing a χ2 function with respect to variations in the model parameters. For the 3k model the data will be fit with kA set equal to 0 in order to test the assumption of the phospholipid irreversible trapping in the tumor membrane. In addition, Patlak plots will be used to standardize the kinetics of tissue radioactivity to the kinetics of plasma radioactivity.
[Para 31 7] Statistical Considerations: This is a feasibility study to determine the tumor uptake and retention characteristics of radioiodinated '24I NM404 by PET-CT in patients with clinically evident metastatic prostate canter. Fifteen patients with metastatic prostate cancer will be accrued for Part 1 of this study. Image scans will be scored using a typical 0-3 scale: 0 = no perceptible uptake, 1 + = uptake barely perceptible above background, 2+ = uptake clearly distinguishable above background, 3+ = intense uptake, much greater than surrounding normal structures (2+ and 3+ are considered abnormal or positive for lesion identification; 0 and 1 + are considered normal or negative for lesion identification). An uptake rate classified as positive for lesion identification of at most 50% would be considered as clinically irrelevant. Therefore, we will test the null hypothesis that the uptake rate classified as positive for lesion identification will be at most 50% versus the alternative hypothesis that the rate is greater than 50%. We anticipate the uptake rate classified as positive for lesion identification will be at least 80%. Assuming a total sample size of 15 patients, the one-sample binomial test with the null hypothesis that the uptake rate classified as positive is at most 50% has 85% power to detect a rate of 80% at the (onesided) 10% significance level. A rate of 90% will be detected with 95% power. With a sample size of 1 5 patients, the proportion of tumors classified as positive for lesion identification will have a standard error of at most 13% and the 90% confidence interval for the proportion will be no wider than 39%.
FPara 31 81 Determine the specific tumor accumulation and metabolic fate of 124I NM404 in patients with clinically organ-confined prostate cancer who are candidates for a radical prostatectomy with bilateral pelvic lymph node dissection.
[Para 319] Objectives and Rationale
[Para 320] As a secondary exploratory analysis, malignant and benign prostatic and nodal tissue obtained by radical prostatectomy and bilateral lymph node dissection will be evaluated for radioiodinated NM404 accumulation and metabolites of NM404 and data from this tissue analysis will be compared with the results of final pathology analysis. Ten patients with organ-confined prostate cancer who are scheduled to undergo a radical prostatectomy with bilateral pelvic lymph node dissection will be enrolled. All patients will undergo conventional preoperative staging studies which include CT scan and bone scan. All patients will also have an NM404 PET-CT scan the week before the scheduled prostatectomy. Final pathologic analysis of the resected prostate and lymph nodes will be correlated with results of the NM404 accumulation and metabolite tissue assay to determine relationship of signal to tumor volume in the primary specimen and lymph nodes in patients with locoregional metastatic disease. Nodal metastases detected by the NM404 accumulation despite a negative preoperative CT scan and bone scan would have great clinical significance in terms of improved staging of these patients prior to treatment. [Para 321 ] Study Procedures: Imaging procedures in this study will be identical to the first study, except that patients will be imaged only once, based upon the optimal imaging time as identified in the first protocol (probably between 24-48 hours after the injection). Six prostate biopsy cores will be obtained in sextant distribution (right and left apex, mid-gland and base regions) from the prostate specimen in the OR immediately following surgical removal. These six biopsy cores will be evaluated by frozen section pathology to ensure a representative sampling of both malignant and benign prostate tissue. A second set of 6 biopsy cores obtained simultaneously from the same corresponding locations in the prostate will be analyzed for uptake of 124I-NM4O4. Accordingly, they will be photographed and undergo high-resolution scanning on a Bioscan AR2000 radioscanner and also weighed and radioactivity quantitated in a well counter. Radioactivity concentration will be determined on a %dose/g tissue sample basis for comparison. These results will be compared to histology results in order to confirm localization in tumor relative to surrounding uninvolved tissue. The core biopsies will be labeled with the patient's study identification number and location in the prostate from which it was obtained.
[Para 322] Final pathology of any lymph nodes harboring metastatic prostate tumor will also be correlated with 524I-NM4O4 uptake signal detected by the preoperative PET-CT scan. Vital signs will be obtained post '24|-NM404 infusion.
[Para 323] Statistical ' Considerations; Ten patients with high-risk clinically localized prostate cancer will be accrued for Part 2 of the study. The null hypothesis for Part 2 is that the log ratio of the accumulation of NM404 in tumor tissues to that in normal surrounding tissues in clinically localized prostate cancer patients is zero. The alternative hypothesis is that the log ratio of the accumulation of NM404 is greater than 0. A tumor-to-adjacent normal tissue (T/N) ratio of at least 5:1 would be considered as clinically relevant. Based on past experience with NM404, it is anticipated that the standard deviation of the log ratio of the accumulation of NM404 is between 1.5 and 2.0. With a sample size of 10, the one-sided t-test with a one-sided 10% significance level has 86%, 90% and 95% power to detect the effect size of 0.8, 0.90, and 1.00, respectively. For example, if the standard deviation of the tog ratio is 2.0, the 5:1 ratio in the accumulation of NM404 in the tumor to normal tissues will be detected with 86% power. Analogously, if the standard deviation of the log ratio is 1.6, a ratio of 5: 1 in the accumulation of NM404 in tumor to normal tissues will be detected with 95% power.
fPara 3241 Determine whether NM404 can detect metastatic recurrence following primary treatment of prostate cancer in ten patients with a rising PSA as their only evidence of disease (stage DO). [Para 325] Objectives and Rationale
[Para 326] By definition, patients with stage DO prostate cancer have a rising PSA following definitive treatment of their disease, signifying biochemical recurrence, with conventional staging studies including CT scan and bone scan which are negative for radiographic evidence of disease. Patients more likely to have disease detectable by the NM404 PET-CT scan will be enrolled such that they must have a PSA rise of greater than 0.75 ng/ml/year.
[Para 327] Study procedures: Imaging procedures in this study will be identical to the first study, except that patients will be imaged only once, based upon the optimal imaging time as identified in the first protocol. Patients will receive an injection of 124I-NM4O4 (0.3 μg/kg body weight, 1 mCi or the limit established by dosimetry calculations in the first set of patients). Patients will also have a PET-CT scan 48 hours after infusion. Vital signs will be obtained post 124I-NM4O4 infusion.
[Para 328] Statistical Considerations: Ten patients with stage DO prostate cancer will be accrued and followed-up for metastatic recurrence for at least 2 years. Since all patients accrued in this part of the study will have PSA values greater than 0.75 ng/ml/year, we expect that at least 60% will experience metastatic recurrence within 2 years. Assuming that 6 out of the 10 patients will eventually experience metastatic recurrence within 2 years, the sensitivity of NM404 to detect metastatic recurrence will be estimated with a standard error of less than 20% and the 90% confidence interval for the sensitivity will be no wider than 55%. Furthermore, the null hypothesis that the sensitivity of NM404 in this patient population is at most 30% will be tested against the alternative that the sensitivity is greater than 30%. The one-sample binomial test has 83% power to detect a sensitivity of 75% at the one-sided 10% significance level. A sensitivity of 80% will be detected with 90% power.
[Para 329] Approximately 230,1 10 new cases of prostate cancer will be diagnosed in the United States for the year 2004 alone. Despite technical refinements in definitive local treatment of clinically organ confined prostate cancer by radical prostatectomy, such that many men are cured with primary therapy alone, as many as 40% of patients will experience biochemical recurrence with long-term follow-up. One of the greatest challenges in treating patients with clinically organ confined prostate cancer or patients with biochemical recurrence following definitive treatment of presumed organ-confined disease remains to accurately distinguish localized versus metastatic disease. This diagnostic capability is important to identify patients who may benefit from effective local treatment modalities including surgery, external beam radiation, brachytherapy, and cryotherapy. Because we presently do not have an accurate means of staging, patients with occult metastatic disease may unnecessarily undergo local treatment with associated risks of therapy. Furthermore, patients with a rising PSA due to local recurrence, in whom systemic recurrence cannot be excluded with confidence, may unnecessarily undergo hormonal ablation, which is generally not considered curative and is associated with osteoporosis development, decreased libido, weight gain, menopausal symptoms, and overall malaise, as well as the evolution of hormonally independent prostate cancer. [Para 330] While conventional imaging studies such as computed tomography (CT) and magnetic resonance imaging (MRI) are useful in assessing soft-tissue metastasis, the vast majority of prostate cancer metastasizes to the bone only. Thus, the utility of CT and MRI scanning in assessing the disease is suboptimal and more sensitive imaging modalities for either locally recurrent or metastatic prostate cancer are necessary. Radioimmunoscintigraphy with lndium-1 1 1 capromab pendetide (ProstaScint, Cytogen Corp, Princeton, NJ) has been utilized in patients following prostatectomy with a rising PSA who have a high clinical suspicion of occult metastatic disease and no clear evidence for metastatic disease in other imaging studies. Use of the ProstaScint scan for patients at risk for occult metastases from prostate cancer remains controversial however. One approach to the development of a sensitive imaging exam is to develop a more appropriate carrier molecule, which is key to achieving delivery of a radiopharmaceutical probe to the desired target tissue. Our strategy has been to study radioiodinated phospholipid ether analogs (PLE) as potential diagnostic imaging agents, to capitalize on the unique biochemical or pharmacological properties of these molecules resulting in a high degree of tissue or tumor selectivity. In preclinical models, we have shown these molecules selectively accumulate in a wide variety of murine and human tumors in high levels.
[Para 331 ] Certain embodiments of the present invnetion provides preliminary data regarding the use of the second-generation PLE analog, NM404, in imaging patients with prostate cancer. It has been shown that NM404 is (a) selectively retained in a wide variety of tumor types in preclinical models, with a high degree of sensitivity, (b) is safe in humans, (c) can be radiolabeled with 1-1 24, and (d) has appropriate dosimetry characteristics labeled with 1-1 31 .
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[Para 375] All references cited herein are specifically incorporated by reference in their entireties and for all purposes as if fully set forth herein. [Para 376] lit is understood that the invention is not limited to the embodiments set forth herein for illustration, but embraces all such forms thereof as come within the scope of the following claims.

Claims

What is claimed is:
[Claim 1 ] A method for detecting and locating recurrence of cancer, radiation and chemo insensitive cancer or metastasis of cancer selected from the group consisting of Lung cancer, Adrenal cancer, Melanoma, Colon cancer, Colorectal cancer, Ovarian cancer, Prostate cancer, Liver cancer, Subcutaneous cancer, Squamous cell cancer, Intestinal cancer, Hepatocellular carcinoma, Retinoblastoma, Cervical cancer, Glioma, Breast cancer and Pancreatic cancer in subject that has or is suspected of having cancer, the method comprising the steps of: administering a phospholipid ether analog to the subject; and determining whether an organ suspected of having recurrence of cancer, radiation insensitive cancer or metastasis of cancer in the subject retains a higher level of the analog than surrounding region(s) wherein a higher retention region indicates detection and location of the recurrence of cancer, radiation and chemo insensitive cancer or metastasis of cancer.
[Claim 2 J The method of claim 1 , wherein the phospholipid analog is selected from:
CH2CH2 Y
where X is selected from the group consisting of radioactive isotopes of halogen; n is an integer between 8 and 30; and Y is selected from the group comprising NH2, NR2, and NR3, wherein R is an alkyl or arylalkyl substituent or
where X is a radioactive isotope of halogen; n is an integer between 8 and 30; Y is selected from the group consisting of H, OH, COOH, COOR and OR, and Z is selected from the group consisting of NH∑, NR2, and NR3, wherein R is an alkyl or arylalkyl substituent.
[Clai m 3] The method of claim 2, wherein X is selected from the group of radioactive halogen isotopes consisting of 18F, 36Cl, 76Br, 77Br, i23|t
124I1 125|r 131 | ancj 2HAt,
[Claim 4] The method of claim 2, wherein the phospholipid ether is! 8-(p- lodophenyOoctadecyl phosphocholine, 1 -O-[l 8-(p-lodophenyl)octadecyl]- 1 ,3-propanediol-3-phosphocholine, or 1 -O-[1 8-(p-lodophenyl)octadecyl]- 2-O-methyl-rac-glycero-3-phosphocholine, wherein iodine is in the form of a radioactive isotope.
[Claim 5] The method of claim 1 , wherein the detection is carried out by a PET, CT, MRI scanning methods and combination thereof.
[Claim 6] A method for the treatment of recurrence of cancer, radiation insensitive and chemo cancer or metastasis of cancer in a subject comprising: administering to the subject an effective amount of a compound comprising a phospholipid ether analog.
[Claim 7] The method of claim 6, wherein the recurrence of cancer, radiation and chemo insensitive cancer or metastasis of cancer occurs in the group selected from Lung cancer, Adrenal cancer, Melanoma, Colon cancer, Colorectal cancer, Ovarian cancer, Prostate cancer, Liver cancer, Subcutaneous cancer, Squamous cell cancer, Intestinal cancer, Hepatocellular carcinoma, Retinoblastoma, Cervical cancer, Glioma, Breast cancer, Pancreatic cancer and Carcinosarcoma.
[Clai m 8] The method of claim 6, wherein the phospholipid analog is selected from:
(CH2JnOPOCH2CH2-
where X is selected from the group consisting of radioactive isotopes of halogen; n is an integer between 8 and 30; and Y is selected from the group comprising NH2, NR2, and NR3, wherein R is an alkyl or arylalkyl substituent or
where X is a radioactive isotope of halogen; n is an integer between 8 and 30; Y is selected from the group consisting of H, OH, COOH, COOR and OR, and Z is selected from the group consisting of NH2, NR2, and NR3, wherein R is an alkyl or arylalkyl substituent.
[Clai m 9] The method of claim 8, wherein X is selected from the group of radioactive halogen isotopes consisting of '8F, 36CI, 76Br, 77Br, 82Br1 1221, 1 23I, '241, 125|, i 3i |( 2i iAt and combinations thereof.
[Claim 1 0] The method of claim 9, wherein the effective amount of phospholipid ether analog is a combination of at least two isotopes, one with a path range of about 0.1 A to 1 mm and a second with a path range of about 1 mm to 1 m.
[Claim 1 l ] The method of claim 10, wherein the effective amount of phospholipid ether analog is a combination of at least two isotopes, 125I and
[Claim 1 2] The method of claim 6, wherein the phospholipid ether isl 8-(p- lodophenyOoctadecyl phosphocholine, l -O-[l 8-(p-lodophenyl)octadecyl]- 1 ,3-prOpanediol-3-phosphocholine, or 1 -0-[I 8-(p-lodophenyl)octadecyl]- 2-0-methyl-rac-glycero-3-phosphocholine, wherein iodine is in the form of a radioactive isotope.
[Claim 1 3] The method of claim 6, wherein the effective amount of phospholipid ether analog is fractionated.
[Claim 1 4] The method of claim 6, wherein the effective amount of phospholipid ether analog is about O.SμCi to about 3Ci treatable in a linear and dose dependent manner.
[Claim 1 5] The method of claim 14, wherein the dosage is adaptable to the cancer-volume.
[Claim 1 6]The method of claim 14, wherein the dosage for radiation and chemo insensitive tumor is greater than dosage for radiation and chemo sensitive tumor and less than 3Ci and is adaptable to the cancer-volume.
[Claim 1 7] The use of phospholipid ether analog for the production of a pharmaceutical composition for the treatment of recurrence of cancer, radiation and chemo insensitive cancer or metastasis of cancer.
[Clai m 1 8]The use of claim 17, wherein the phospholipid analog is selected from:
where X is selected from the group consisting of radioactive isotopes of halogen; n is an integer between 8 and 30; and Y is selected from the group comprising NH2, NR2, and NR3, wherein R is an alkyl or arylalkyl substituent or
where X is a radioactive isotope of halogen; n is an integer between 8 and 30; Y is selected from the group consisting of H, OH, COOH, COOR and OR, and Z is selected from the group consisting of NH2, NR2, and NR3, wherein R is an alkyl or arylalkyl substituent.
[Claim 1 9] The use of claim 1 8, wherein X is selected from the group of radioactive halogen isotopes consisting Of 18F, 36CI, 76Br, 77Br, 82Br1 1221, 123I, i24|r i25|( 1311, 2i iAt and combinations thereof.
[Claim 20] The use of claim 1 7, wherein the phospholipid ether isl 8-(p- lodophenyOoctadecyl phosphocholine, 1 -0-[l 8-(p-lodophenyl)octadecyl]- 1 ,3-propanediol-3~phosphocholine, or 1 -0-[l 8-(p-lodophenyl)octadecyl]- 2-O-methyl-rac-glycero-3-phosphocholine, wherein iodine is in the form of a radioactive isotope.
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Inventor name: WEICHERT, JAMEY

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