Classifications

• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
  • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
  • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
  • B09C—RECLAMATION OF CONTAMINATED SOIL
  • B09C1/00—Reclamation of contaminated soil
  • B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
• • C—CHEMISTRY; METALLURGY
  • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
  • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
  • C02F3/00—Biological treatment of water, waste water, or sewage
  • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
• • C—CHEMISTRY; METALLURGY
  • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
  • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
  • C02F3/00—Biological treatment of water, waste water, or sewage
  • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
  • C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
  • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
  • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
  • C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
  • C12N11/12—Cellulose or derivatives thereof
• • C—CHEMISTRY; METALLURGY
  • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
  • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
  • C02F2305/00—Use of specific compounds during water treatment
  • C02F2305/06—Nutrients for stimulating the growth of microorganisms

Definitions

• the invention relates to a biodegradable product for environmental biotreatment.
• Pollution and environmental nuisance for which biological treatments are required are very varied: it can be the destruction of organic pollutants such as hydrocarbons, the destruction of associated inorganic components, the removal of olfactory nuisances and others, in the case of accelerated composting of organic waste from household waste or green waste or the treatment of pollution contained in waste water and, more generally, the treatment of lipid, carbohydrate or protein compounds.
• bacterial strains such as, for example, inert solid supports or organic active supports impregnated with said bacterial strains, or else encapsulation techniques allowing packaging in spaces. closed, bacterial colonies.
• compositions in solid forms that are intended to release organic substances and inorganic substances in a medium to be treated, in general, a soil.
• These solid compositions may be in the form of granules, briquettes, capsules, powders or any other form capable of being stored and then easily dispersed in the medium to be treated.
• a first document (US 6,620,611) describes a substrate impregnated with an active composition intended to diffuse over time organic compounds and complex inorganic phosphates for the purpose of biotreatment of a soil.
• a patent application (US 2002/0090697) describes a solid chemical composition with a degradation and slow diffusion effect, as well as a method for the biotreatment of soils with a view to their restoration by techniques using anaerobic bacteria.
• a recent paper (US 6,251,826) describes a composition for soil treatment application with the addition of compost, • to promote good plant growth without the use of pesticides.
• the composition uses a crushed alfalfa, a wetting agent, a source of calcium, and a humus, in the form of granules. But this composition does not contain bacterial strains. Therefore, there is no bacterial selection and the purpose of this composition is the enrichment of soil and not the treatment of a specific environmental pollution.
• the object of the invention to meet needs not '' satisfied 'is to create a biodegradable product for environmental biotreatment with the functions: (i) contain or / and provide support to colony of bacteria • aerobic chosen very specific way with respect to the final use will be made of it, (ii) protect these bacteria during conditioning, (iii) maintain the presence of oxygen in the conditioning space to to preserve the ability of bacteria to act when the time comes, thanks to a sufficient permeability to oxygen, (iv) to offer the necessary nutrients for bacterial growth when the time comes, (v) to hydrolyze in the medium receptor, (vi) biodegrades in a controlled manner when the conditions of use are satisfied, in particular with sufficient moisture, (vii) to release the aerobic bacterial colony while ensuring its growth at the site of its biotreatment action program.
• the object of the invention is a biodegradable product for environmental biotreatment with controlled biodegradability, which is in any convenient physical form, such as, for example, a 1 wrapping or the like, a capsule, a capsule wherein 'the inoculated bacteria are aerobic bacteria selected very specifically for a given action with respect - to an environment known to degrade by this bacterial colony.
• the environmental bioprocessing product combines the conditioning, carrier and nutrient functions for the aerobic bacterial colony, and integrates its own controlled destruction, releasing the bacterial colony into the environment to be treated by means of a biodegradable behavior. specifically designed.
• the invention relates to a biodegradable product for environmental biotreatment.
• the biodegradable product for environmental biotreatment is characterized in that it consists: a) a packaging envelope permeable to oxygen, carried out by means of • a biodegradable composition comprising: al) at least one crushed fibrous plant material, a2) at least one binding agent of natural origin or of synthetic origin and a3) at least one plasticizing agent,
• a formulated content of the envelope, placed inside said conditioning envelope composed: b1) of a culture containing bacteria, fungi and / or aerobic yeasts b2) of a nutrient substrate containing at least least one amino acid acting as an osmotic membrane and as a nutritive medium for bacteria at the time of revival and b3) at least one viscosity modifier.
• the biodegradable product for the environmental biotreatment firstly comprises a packaging envelope made using a biodegradable composition comprising at least one fibrous ground vegetable material, at least one binder of this material and at least one plasticizing agent. and may contain other adjuvants and additives and then formulated content, internal to the packaging casing, whose formulation comprises an aerobic bacterial culture, a nutrient substrate and at least one viscosity modifier.
• biotreatment in particular bioprocessing with an environmental vocation, using bacteria, fungi and / or aerobic juices, it is essential to ensure the presence of oxygen for the culture containing the bacteria. , fungi and / or aerobic yeasts contained in the biodegradable packaging envelope. This is the reason why this packaging has been chosen preferentially in the form of an envelope whose walls are sufficiently porous with oxygen, while remaining impervious to liquids.
• the biodegradable composition for the production of an envelope of packaging comprises as an essential component, at least one milled plant fiber material .lui 'allowing it to be implemented in the form of tubes, profiles, sheets monolayers or multilayers, further processed in envelopes, at least one binding agent, at least one plasticizing agent and may also comprise other compounds such as adjuvants and additives, nutrients, none of which must therefore restrict the performance of the controlled biodegradability of the envelope .
• the biodegradable composition making it possible to produce the packaging envelope first comprises, at least, a crushed fibrous plant material.
• biodegradable materials While many biodegradable materials exist, they are, in most cases, obtained from essentially starch, with possible incorporation of various binders, both natural and synthetic, but their oxygen porosity properties are not readily available. sufficient to ensure the durability of aerobic bacteria during storage. Any packaging envelope intended to receive an aerobic bacterial colony, must indeed have different characteristics, in particular, a good oxygen permeability or a sufficient oxygen reserve, a good biodegradability activated in the presence of moisture, a capacity to have a low osmotic pressure when this packaging is in the form of an envelope separating the external medium from the internal medium containing the bacteria to be protected.
• This packaging envelope can be in different physical forms. according to the uses that will be made of it: it may be in the form of shaped sheets in the form of envelopes, in the form of capsules or capsules, or in the form of closed tubes or profiles at the ends in which the colony of bacteria, fungi and / or aerobic yeasts will be contained.
• fibrous plant material as the main element of the biodegradable composition has appeared to be indispensable.
• This fibrous plant material is associated with at least one binder and at least one plasticizer, to obtain a material having a certain plasticity that allows the formation of a conditioning envelope for the aerobic bacterial colony.
• This fibrous plant material provides mechanical reinforcement, porosity supplement and nutrient.
• the fibrous plant material used as the main component of the biodegradable composition is selected from the group of plants comprising alfalfa, hemp, flax residues, beet snares, lupines and faba bean legumes.
• alfalfa is used as a component the
• Alfalfa is preferentially chosen in that it has certain special characteristics compared to other plant compounds, namely, that of not containing starch and that of containing hemicellulose easily assimilated by aerobic bacteria and not of the only cellulose more difficult to assimilate.
• the fibrous plant material is both a structuring element of the composition as can be any reinforcing fiber, but also a nutrient for aerobic bacteria and an element for binding part, thanks to its protein part, however insufficient to her only.
• the protective packaging envelope of the aerobic bacterial colony also serves as a nutrient for these bacteria, before and when they will be released in order to act on the medium to be treated.
• the fibrous plant material is physically treated such as drying, dehydration, grinding, compaction, pressing.
• the at least one fibrous plant material is introduced in a proportion of 10 to 90% by weight and preferably 20 to 45% by weight relative to the total weight of said biodegradable composition.
• the biodegradable composition making it possible to produce the packaging envelope also comprises at least one binding agent for the one or more fibrous plant materials.
• the role of the binding agent in the biodegradable composition is to allow the fibrous plant constituents to cohere with one another, but this binding agent can have a cohesive power such that the material loses all ability to behave in a plastic manner, for example, when subjected to a rise in temperature or when put in the presence of water ..
• the binding agent used may be of natural or synthetic origin and must in all cases be rapidly biodegradable.
• the binder is' mainly composed of a mixture comprising starch and proteins.
• the 'proportions of starch and protein are from 5 to 25% by weight protein and 75 to 95% by weight of starch.
• Plants forming amylaceous compounds and acting as a binding agent are selected from the group of plants such as rice, potatoes, maize, cassava, wheat, hops, oats, barley, tapioca.
• the naturally occurring binder is flour derived from cereals such as wheat.
• This naturally occurring binder comprises starch and proteins derived from gluten in the case of wheat flour.
• This natural binder has proven to be possible in so far as such a flour provides cohesion to the composition, without restricting, if the addition is modest, s permeability properties to oxygen envelope packaging made from the composition.
• the naturally occurring binding agent comprising starch and proteins is added to the composition at 25% to 47% by weight of the biodegradable composition of the shell.
• Another water-soluble carbon binding agent also of natural origin, can be associated with the first previously described. It is selected from green juices such as alfalfa products or other plants including grasses, polysaccharides from products such as molasses, cane sugar and / or beetroot, products from green juice splits such . than hydrolysates and sera.
• this other natural binding agent associated with the first consisting of starch and protein is interesting in that it provides, beyond its primary function of binding, a certain plasticity to the composition.
• this other binding agent provides additional organic carbon which is advantageous as a nutrient present in the packaging envelope because this nutrient is easily assimilated by the bacterial colony, and / or fungi and / or yeasts, since it is sugar.
• This other water-soluble carbonaceous binder is added in a proportion of from 0% to 5% by weight of the biodegradable composition of the shell.
• binding agents of synthetic origin in substitution for all or part of binding agents of natural origin, such as water-soluble polymers, may optionally be used, preferably polyvinyl alcohols. These binding agents of synthetic origin may be used in an amount chosen in the range of 0% to 40% by weight and more particularly in the range of 5 to 40% by weight of the biodegradable composition constituting the envelope.
• the biodegradable composition for producing the packaging envelope also comprises at least one plasticizing agent, of natural or synthetic origin.
• thermoplastic biodegradable composition that can be easily converted, for example, into tubes, profiles or monolayer or multilayer sheets, then into packaging envelopes by techniques borrowed from the processing of plastic materials or the processing of plastics. extrudates qualified food, then implemented by available techniques such as cutting, forming, welding and which can be sufficiently resistant once formatted, the biodegradable composition should be formulated with various' natural organic plasticizers and / or synthetic, preferably of limited molecular weight, capable of being soluble in water and of being themselves also biodegraded, without residual ecotoxicity when dispersed in the medium to be treated.
• the biodegradable composition constituted by the natural fibers and the binding agents must have a certain plasticity with respect to the forming stresses.
• the role of the plasticizer is to create volumes of freedom in Macromolecules ⁇ é vide so that under the effect en- particular heat, the constituent macromolecules of the composition can move more easily relative to each other .
• the plasticizer also operates at room temperature. It provides the necessary flexibility to the envelope resulting from the composition implemented which, without the presence of said plasticizer, would be too rigid and possibly brittle.
• Water-soluble plasticizers are selected from the group • polyols, including in particular glycerol, sorbitol, ethylene glycol, diethylene glycol, propylene glycol.
• polyols including in particular glycerol, sorbitol, ethylene glycol, diethylene glycol, propylene glycol.
• Other polyols are also possible such as . adonitol, dithioerythritol, dithiothreitol, galactitol, inositol, mannitol, xylitol. All these polyols can be used alone or as a mixture.
• the plasticizing agent of polyol type is added in proportions of 1% to 30%, and preferably 3% to 20% by weight of the biodegradable composition, in this case the envelope.
• the biodegradable composition for producing the packaging casing may also comprise other compounds which are adjuvants and additives such as salts, lubricating agents, mineral fillers, colored dyes or pigments. .
• Salts may be added to the biodegradable composition, particularly, when these salts are hygroscopic behavior to maintain within the biodegradable composition% a sufficient moisture promoting the plasticity of said composition.
• salts are selected from the group consisting of salts of lactic acid, in particular sodium lactate or potassium lactate. The addition of salts is done in the
• Lubricants may also be added to the biodegradable composition for the purpose of making it more readily convertible and in particular extrudable and more amenable to mixing in extruder screws.
• Such lubricating agents are well known in the state of the art and are selected from the group consisting of the metal salts of stearic acid, mainly the sodium, magnesium and potassium salts, but also aluminum, calcium, zinc or other metals replacing sodium or potassium. Magnesium stearate is preferably selected.
• lubricating agents are present in the composition in proportions of 0% to 5% and preferably 0.5% to 3.5% by weight of the biodegradable composition.
• Mineral fillers may also be added to control the viscosity of the transformable biodegradable composition. These mineral fillers may be clay, silica, talc, calcium carbonate, titanium oxide or any other neutral charge with regard to the ecosystem, taken alone or as a mixture.
• a clay is preferably chosen insofar as it contains water of constitution and has, moreover, the capacity to absorb it and that it also acts as a modifier of rheology, that is to say of viscosity and as a "possible support of the bacterial colony once the packaging biodegraded packaging.
• the clay selected is preferably a 'phyllosilicate and more particularly, a kaolinite whose structure is. composed of silicate layers associated with aluminum oxide / hydroxide layers or montmorillonite.
• the proportion of mineral fillers introduced into the biodegradable composition varies between 0% and 15% and preferably between 0% and 10% by weight of the transformable biodegradable composition, the size of the particles being 0 simply micrometric.
• a coloring pigment may optionally be added subject to its environmental non-toxicity when released, during the biodegradation of the composition in the environment: the titanium oxide is preferably retained.
• additives may be added to the biodegradable composition such as nutrients, bacterial growth additives, enzymes, amino acids, mineral salts or ammonia.
• Specific nutrients and growth additives may be selected from pectins, yeast extracts, polysaccharides from the wine industry, hemicellulose, optionally cellulose.
• Enzymes, amino acids, vitamins can be added to the biodegradable composition.
• Mineral salts can be added to the biodegradable composition. Their nature and quantities are carefully studied. These mineral salts are nitrate compounds and / or phosphates and / or sulphates of ammonium, potassium, magnesium, calcium, sodium, manganese, molybdenum, iron, copper, zinc.
• the biodegradable composition for producing the packaging casing comprises a water content which varies between 5% and 20% by weight of the convertible biodegradable composition, which may be water already contained in the fibrous plant material and / or supplemented by a contribution.
• the conditioning envelope of the aerobic bacterial colony is preferably in the form of a capsule made from tubes, sections, monolayer or multilayer sheets, extruded, injected or calendered from the biodegradable composition according to the invention, hot-glued on their periphery or at their ends.
• the capsule having received its formulated content is closed by pinching at both ends in the case of tubes and
• the material which constitutes the outer envelope of the capsule is always permeable to oxygen.
• the biodegradable product for environmental biotreatment is thus a capsule, of variable size, having received its formulated content which is stable and which can be easily stored and handled, to then be used, when the time comes, under conditions of humidity allowing its delitescence and its controlled biodegradation, the reviviscence of aerobic bacteria and thus their release and their effective use in the medium to be treated.
• the formulated content of the envelope b) consists of: • .
• the bacteria, fungi, and / or aerobic yeasts present in the contents of the envelope are of the nitrogen and carbon cycle and are selected from the group consisting of Micrococcaceae, Lactobacillaceae, ' Bacillaceae ' , Nesseriaceae, Enterobacteriaceae, Azotobacteriaceae, Rhizobiaceae, Nitrobacteriaceae, Thiobacteriaceae, Pseudomonadaceae, as well as in the groups of Bacteria: Pseudomonadales: Pseudomonas, Nitrosomonas, Nitrobacter, Thiobacillus, Vibrio, Acetobacter, ...
• Azotobacteriaceae Azotobacteria, Beijerinckia Achromobacteriaceae: Achromobacter, Flavobacterium Enterobacteriaceae: Aerobacter, Serratia Lactobacillaceae: Lactobacillus, Corynebacteriaceae: Arthrobacter Bacillaceae: Bacillus, Actinomycetes: Actinomycetaceae: Nocardia, Pseudonocardia, Mushroom fungi: Rhizopus, Protoascomycetes (yeasts): Candida, Torula, Rhodotorula, Cryptococcus, Torulopsis, Euascomycetes (molds): Aspergillus.
• the formulated content of the envelope comprises between 0.5% and 25% and preferably between 1% and 10% of aerobic strains, that is to say bacteria, fungi and / or aerobic yeast of the nitrogen cycle and carbon in their culture in an aqueous medium, with the
• the nutrient substrate comprising at least one amino acid is produced by means of a formulated aqueous phase in which certain components are soluble, in particular amino acids.
• a formulated aqueous phase in which certain components are soluble, in particular amino acids.
• One or more of the 21 amino acids can be used.
• the amount of amino acids present in the nutrient substrate is from 1% to 10% by weight of said nutrient substrate or from 0.2% to 7% by weight of the formulated content of the envelope.
• amino acids have the property of acting as an osmotic membrane and as a nourishing medium for the bacteria contained at the moment of their revival.
• Mineral salts and / or ammonia may be added to the formulation of the contents of the envelope. Their nature and quantities have been carefully studied. These inorganic salts are nitrates and / or phosphates and / or sulphates of ammonium, potassium, magnesium, calcium, sodium, manganese, molybdenum, iron, copper and zinc.
• Nutrients and bacterial growth additives can be added to the formulated content of the envelope. These nutrients may be selected from pectins, yeast extracts, pqlysaccharides, vitamins, hemicellulose, optionally cellulose, enzymes.
• a viscosity modifying agent acting as a thickener may be added to modify the viscosity of the formulated content of the envelope to facilitate its implementation in the process of transformation.
• This viscosity modifying agent may be of mineral or organic origin.
• clay When it is of mineral origin, it acts as a mineral charge; it may be a calcium carbonate, a magnesium carbonate, a silica, a clay and is preferably a clay.
• This clay is kaolinite type 'or montmorillonite. The size of the clay particles is micrometric. In addition, the nature of these clays facilitates the adsorption of colonies of bacteria, fungi and / or aerobic yeasts.
• the viscosity modifying agent when the viscosity modifying agent is of organic origin, it may be selected from the group. glutamates, carrageenans or alginates such as agar.
• Viscosity modifying agents may be taken alone or in admixture.
• Bacteria implemented according to the invention and therefore 'inoculated in the formulated internal contents are aerobic strains, such as bacteria, yeasts, fungi of the nitrogen cycle and carbon. They can be from the same family or belong to different families
• the culture of aerobic strains including bacteria, fungi and / or yeasts is done in an aqueous medium at a concentration ranging for example between 10 7 and 10 10 each forming a colony per milliliter (cfu / ml) of aqueous suspension.
• the quantity of this bacterial culture introduced into the formulated content of the envelope represents between 0.5% and 20% by weight and preferably between 1% and 10% by weight of said formulated content.
• the internal content formulated of the envelope represents between 5% and 45% and preferentially between 10% and 30% of the weight of the
• biodegradable product of environmental biotreatment and the packaging envelope represents between 95% and 55% by weight and preferably between 90% and 70% by weight of the biodegradable product.
• the process for producing biodegradable products for biological treatment, in particular for environmental biotreatment is characterized in that it comprises the following steps:
• the process for manufacturing the biodegradable product of environmental biotreatment comprises a first step a) of preparation of the fibrous plant material, drying, possible dehydration, grinding to the dimensions making it possible to produce a paste of satisfactory viscosity so that it can be transformed into an envelope, for example a tube, a profile monolayer sheet or multilayer wall thickness of the order of a few tenths of millimeters to a few millimeters.
• the fibers of the fibrous plant material should not be too long because the envelope thus formed would lose in homogeneity and there would be a risk of formation and presence of micro-holes in said packaging envelope prepared by transformation of the composition, thus no longer allowing to preserve the bacterial colony since there would be loss of tightness. It is really important to be able to produce a biodegradable product according to the invention which is flexible, continuous and homogeneous.
• the dimension of the fibers is also important for the implementation by a transformation means such as extrusion, compounding, coextrusion, tubes, profiles, single or multilayer sheets, in film or injection molding.
• the plant material fibers have a size that varies from a few microns to a few tens of millimeters, but the size distribution is essentially between 0.5 and 15 mm and preferably between 1 and 10 mm.
• the role of the fibrous plant material is also to enhance the mechanical properties of the biodegradable composition, independently of the other functions provided.
• the supply of this fibrous plant material to the biodegradable composition can be in the form of dried material, ground, possibly dehydrated and also in a compacted form.
• the relative humidity level of fibrous plant materials is an important element in controlling the performance of the biodegradable composition throughout the manufacturing process of the packaging envelope and its implementation. Relatively low relative humidity will result in
• a relative humidity of 5% to 30% and preferably from 5% to 15% expressed as • weight of the composition is desired.
• the process for producing the biodegradable environmental biotreatment product comprises a step b) which is that of the preparation of the biodegradable composition, in the form of a homogeneous mixture.
• the biodegradable organic binding agent (s) and / or the plasticizing agents are added for their mixing.
• the control of the moisture content of the biodegradable composition makes it possible to obtain, after processing by the techniques of the plastics industry or the food industry, a relatively plastic paste, viscosity suitable for implementation processes such as extrusion / coextrusion, forming in a die or other, then passage on cooling and forming means such as rollers, rollers, confofmateurs.
• a mineral filler may also be added in powder form to better control the viscosity of the product being processed such as by extrusion, coextrusion, forming or the like.
• the clay is preferably retained to the extent that such a material has the ability to bind bacteria, yeasts, fungi, when using the biodegradable composition.
• the preparation step of the biodegradable composition can be done in any mixer as available in the industry : plastics processing or the food industry.
• the optimal homogenization is done in a turbine mixer where the various organic and inorganic ingredients will be added. There is no incident temperature rise during mixing. It can also be done directly in a compounding extruder having sufficient capacity to mix and homogenize the composition which allows sequential introductions of the various solid and liquid components.
• the process for producing the biodegradable biotreatment product comprises a step c) of preparing a formulation of the contents of the envelope to be introduced into the envelope, comprising a bacterial colony, aerobic yeasts and / or fungi, a nutrient substrate containing at least one amino acid and at least one viscosity modifier, to be packaged in the packaging casing.
• This internal content formulated of the envelope represents between 5 and 35% by weight of the biodegradable product of environmental bioprocessing according to the invention.
• This formulation of the content of the biodegradable envelope is based on amino acids and optionally mineral salts, growth additives and nutrients such as polysaccharides and clay suitable for containing the bacterial colony.
• the amino acids and possibly the other nutrient substrate additives, such as polysaccharides, have the primary function of providing an additional nutrient to the aerobic bacterial colony, but another function of the internal formulated content partly to coat the inner surface of the aerobic bacterial colony.
• envelope is to change the osmotic pressure relative to the aqueous mixture of the solution containing the bacterial colony and also provide some sealing of the biodegradable composition to better control its ability to biodegrade and promoting, thanks to polysaccharides, conservation of bacteria by • the 'sugars easily assimilated.
• the 21 amino acids can be used alone or in mixture.
• the preferred amino acids are arginine, methionine, isoleucine, tryptophan, which can represent between 70% and 80% by weight of all the amino acids.
• Nutrient uptake constitutes between 20% and 70% of the weight of the formulated internal content of the envelope.
• the viscosity of this formulated internal content constituted by the aqueous phase of amino acids and optionally of polysaccharides and other additives is modified by the addition of a viscosity modifying agent which may be of mineral or organic origin.
• a viscosity modifying agent which may be of mineral or organic origin.
• the viscosity-modifying agent may be chosen from the group of silicas, carbonates and clays and is preferably chosen from clays.
• the viscosity modifying agent is of organic origin, it is chosen from the group of glutamates, carrageenans or alginates, for example agar.
• viscosity modifiers are used to control the viscosity ', in particular, thickening when implemented by injection, by pumping or lateral extrusion in the die 5 of the co-extruder, the composition referred to constitute the internal content formulated from the envelope during coextrusion.
• This viscosity modifying agent constitutes between 20% and 70% by weight of the formulated internal content of the envelope.
• an aerobic bacterial colony is introduced in the form of a dispersion in a nutrient liquid medium.
• the conditions for introduction of the bacterial colony involve preparation of the aqueous medium of the bacterial colony, pre-introduction of the selected bacterial strains into that aqueous medium and introduction, into the formulation of the formulated internal content, of the strains and . growth medium before injection, pumping, extrusion, in a coextrusion unit for example at the level of the coextrusion die before the forming phase of the envelope such as tube, profile, single sheet or multilayer or other.
• the envelope such as tube, profile, single or multilayer sheet or the like
• a formulation in which the bacterial colony is located is filled with a formulation in which the bacterial colony is located.
• the bacterial colony is selected according to the types of use
• viscosity of the bacterial solution is chosen so that the introduction is easy, that it can be carried out without discontinuity and that the
• the concentration of bacteria, fungi and yeasts is 10 7 to 10 10 colony forming units per milliliter of suspension aqueous culture (cfu / ml) and the amount by weight of culture is variable depending on the application.
• lipids, proteins, bacteria, yeasts and fungi produced sequentially sterile fermenters are premixed and conditioned with the mineral salts and oligo ⁇ elements constituting the growth medium.
• bacterial populations include many genres and families including 'those listed in Table 1.
• Yeasts belong to the family Saccharomycetes, Myxomycetes, Candida. Fungi are used for the degradation of certain lipid compounds.
• the concentration of total heterotrophic seeds is 10 7 to 10 10 cfu / ml; the amount of culture used in the biodegradable product of the invention varies according to the use.
• the mass constituted by the aerobic bacterial colony, fungi and / or yeasts in aqueous medium varies between 0.5% and 20% and preferably between 1% and 15% by weight of the total mass of the internal content formulated envelope.
• the bacteria present in the formulated internal fodder are at a concentration of 10 7 to 10 10 cfu / ml.
• This internal content formulated based on amino acids and possibly polysaccharides, a modifying agent viscosity and containing the bacterial colony inoculated, completely filled the biodegradable envelope such as tube, profile, monolayer sheet or multilayer or other and also acts as a coating of the inner faces of said envelope such as tube, profile, single sheet or multilayer or other issue of the composition.
• the biodegradable product manufacturing method biotreatment environnemerîtal comprises a step d) at least partial transformation of wrap of 'the biodegradable composition by compounding, lamination and forming said biodegradable composition. All processing technologies specific to the plastics industry and the food industry and more particularly those of extrusion and coextrusion, single screw, bi screw are possible.
• co-injection and multiple calendering technologies can be used.
• prior compounding extrusion is required.
• the composition is moisture sensitive, it can not be cooled with water to prepare intermediate granules which would then be reheated and plasticized to produce the packaging casing. This is why the cooling is done by means of air which can itself be cooled.
• the extrusion compounding process and the forming processes are carried out at controlled temperature and pressure. Any technique for manufacturing multilayer or multi-wall type products and using different materials to constitute in one operation, a multi-component product, as available for the implementation of the polymeric materials or food products said extruded or coextruded, is adapted to implement the biodegradable composition ' to manufacture ' such an envelope receiving the internal content formulated.
• the biodegradable composition can pass from a pasty state to a viscoelastic solid, without being brittle. There is a change of modulus of elasticity as a function of moisture content while keeping a softening ability at certain temperatures suitable for any thermoplastic behavior. It is necessary to avoid any risk of thermal degradation of the organic materials of the biodegradable composition, such as, for example, Maillard reactions or carbonizations caused by premature oxidation.
• the retained screw profile must allow a good homogenization of the composition, without inducing a too high shear rate which would result in an excessive increase of the temperature of the composition.
• the extruder may be a co-rotating or counter-rotating twin-screw machine or a single-screw machine. The best compounding results are obtained with a co-rotating twin-screw.
• the use of a BUSS type compounder with two perpendicular extruders is also possible.
• the temperature of extrusion in the compounding extrusion technology is between 8O 0 C and 13O 0 C. Too high a temperature results in the loss of the specific properties obtained through the provision of plant materials and optionally generates the creation of Maillard reactions, resulting from the possible presence of sugar.
• Degassing is optionally used to control the water vapor generated during the extrusion of the components of the biodegradable composition whose moisture content would be too high, particularly with regard to plant products.
• the biodegradable thermoplastic composition coextruded at relatively low temperature is converted into at least a partial envelope in a die which can. be annular in the case of a tube, square, rectangular or more generally of polygonal section in the case of a profile or flat with a single lip in the case of a monolayer sheet but with several lips, in the case of a multilayer sheet whose inner layer sandwiched between the two outer sheets would be constituted by the internal content formulated.
• the thicknesses of walls or leaves range from a few tenths of millimeters to a few millimeters.
• This envelope such. that tube, profile or single or multilayer sheet or the like is cooled between two tractive-cooling means such as cylinders, rollers, shapers.
• This implementation can be done on a single machine or in recovery, the extruder or co-extruder then being fed from prefabricated granules in an independent compounding extruder.
• the temperature of the casing such as tube, profile, monolayer or multilayer sheet or others at the outlet of the die lips is less than 100 ° C.
• the thickness of the walls of the tube, shaped, multilayer sheet varies between 0.15 mm and 5 mm, but is preferably between 0.5 mm and 3 mm, to ensure relative strength.
• the flow rate is essentially a function of the extruder / co- extruder and cooling capabilities of the rolls, rollers, cooling shaper and the thickness of the product thus extruded or coextruded.
• the at least partial envelope, when the coextrusion conditions are effectively controlled, is a product that remains flexible, before cooling, in order to be handled without risk of rupture and without performing too low abrasion performance.
• the water content is controlled in a range of 5% to 30% by weight of the biodegradable composition of the shell.
• the injection of water is a means of modifying the water content of the composition, which must keep plasticity until the closing step by forming / plating / welding the tube, profile, single sheet or multilayer or other.
• the process for manufacturing products intended for environmental biotreatment comprises a step e) of homogenization and introduction by injection, pumping or other, of the formulation constituting the content formulated inside the at least partial envelope such as tube , Profile, monolayer sheet or multilayer or other, formed during the transformation operation of the biodegradable composition to achieve the at least partial envelope such as tube, profile, single sheet or multilayer or other receiving the internal content formulated.
• the i-njection into the envelope of the formulated internal content takes place at the level of the coextrusion die. This injection can be done through various means which the
• the viscosity of the formulated internal content is compatible with the flow requirements in the pumping systems, on the one hand and is, on the other hand, adjusted so that the formulated internal contents can not flow out of its envelope not yet closed, at the exit of the die.
• the control of the temperature of the internal content formulated, during its injection into the coextrusion die is such that it avoids any risk of destruction of the bacterial colony present in the forage that would result from too high a temperature.
• the injection rates of the semi-pasty formulation constituting the internal content formulated are controlled and slaved to the main extruder flow implementing the biodegradable composition to form the envelope.
• the process for manufacturing products intended for environmental biotreatment comprises a step f) of sealing the envelope by pressure exerted on the parts or the ends of the envelope such as tube, profile, single or multilayer sheet or other, charged with internal content formulated and cuts filled envelopes thus formed.
• the envelope as tube, roll-formed sheet or multilayer mono or other charge of the formulated internal content after cooling is passed between two rollers and is shaped 'and pinched' in these two ends, in the case of a tube or profile or at both ends and on both sides in the case of a multilayer sheet.
• a welding bonding is carried out the
• the two pinched ends may be parallel or at different angles.
• This forming makes it possible to constitute objects of type for example, berlingots, alveoli, capsules, filled, ranging from 1 cm side to a few centimeters on the side, or even a few tens of centimeters depending on the final applications considered. all
• a continuous production line of environmental bioprocessing product, in the form of capsules has been designed.
• the conditioning envelope of the aerobic bacterial colony is preferably in the form of a capsule made from two sheets from the previously described composition, hot-glued on their periphery, previously coated, on their inner side, a formulated coating comprising in particular amino acids, and receiving the colony of aerobic bacteria, and / or fungi and / or yeasts present in an aqueous medium.
• This coating of the inner surface of the packaging envelope which constitutes at least a part of the internal content formulated (b) of said envelope is produced by means of a formulated aqueous phase, in which certain components are added and comprising in particular the amino acids previously stated.
• a formulated aqueous phase in which certain components are added and comprising in particular the amino acids previously stated.
• One or more of the 21 amino acids can be used.
• the amounts of amino acids deposited in this form of coating are 100 to 5000 g / m2.
• inorganic salts such as nitrates and / or phosphates and / or sulphates of ammonium, potassium,
• Magnesium calcium, sodium, manganese, molybdenum, iron, copper, zinc,
• Nutrients and bacterial growth additives selected from pectins, yeast extracts, the
• polysaccharides polysaccharides, vitamins, hemicellulose, possibly cellulose, enzymes.
• the internal coating represents between 5 and 35% and preferably between 10 and 35% of the weight of the biodegradable product of environmental biotreatment constituted by the packaging envelope, the solution of bacteria, fungi and yeasts, the coating of amino acids and the viscosity modification clay.
• Clay is added to modify the viscosity of the aqueous phase intended for said inner coating in order to facilitate the deposition, this clay being of kaolinite or montmorillonite type.
• the families of bacterial colonies used according to the invention are aerobic strains, such as bacteria, yeasts, fungi of the nitrogen cycle and carbon.
• aerobic strains such as bacteria, yeasts, fungi of the nitrogen cycle and carbon.
• the quantities of strains introduced into the envelope are aerobic strains, such as bacteria, yeasts, fungi of the nitrogen cycle and carbon.
• D of conditioning are from 0.0001% to 2% by weight relative to the aqueous medium.
• the amount of bacterial culture represents between 2 and 20% by weight of the biodegradable product of environmental bioprocessing.
• the process for manufacturing biodegradable products, according to the invention, intended for biological treatment, in particular environmental biotreatment is characterized in that it comprises the following steps:
• the compounding step forming plasticizing d) • may be a single continuous step or be discontinuous, after the engine has available, compounding twin-screw extruder or single screw extruder mono involving the prior manufacture of granules of the composition that will have to be plasticized and put in the pasty state again.
• the water content is controlled in a range of 5 to 30% expressed by weight of the biodegradable composition.
• the injection of water is a means of modifying the water content of the composition, which must keep a plasticity until the thermoforming step of the sheet and welding.
• the process for manufacturing products intended for environmental biotreatment comprises a step g) of forming a sheet constituting the lower part of the packaging envelope.
• This sheet resulting from the composition is thermoformed, preferably just after its
• thermoforming extrusion, before the coating phase by a formulated coating comprising in particular amino acids.
• the forming is actually facilitated when the sheet still has a certain plasticity. If not, it is necessary to proceed to a warming of this sheet, to be able to perform this thermoforming.
• Thermopolding is carried out on a depth ranging from 5 mm to 3 cm for cell dimensions ranging from 1 cm on the side " to a few centimeters on the side, or even a few tens of centimeters depending on the final applications considered.
• the constraints specific to the thermoforming of thermoplastic sheets whose modulus of rigidity is variable with temperature make the shapes rounded and relatively shallow in relation to the size of the cell will be preferred.
• Environmental biotreatment biodegradable • manufacturing process comprises a step h) of preparing a formulation based on amino acids and optionally minerals, growth additives and nutrients such as polysaccharides and clay , intended to constitute a coating.
• Amino acids and possibly other additives such as polysaccharides have the primary function of providing an extra nutrient to the aerobic bacterial colony. Another function of this coating is to modify the osmotic pressure with respect to the aqueous mass of the solution containing the bacterial colony, and also to provide some waterproofing of the biodegradable composition to better control its biodegradability and to promote , thanks to polysaccharides, the conservation of
• the formulated internal coating represents between 10 and 25% by weight of the biodegradable environmental biotreatment product according to the invention.
• the viscosity of this coating consisting of the aqueous phase of amino acids and optionally polysaccharides and other additives may be optionally modified by the addition of clay.
• the 'method' product manufacturing biodegradable environmental biotreatment comprises a step f) of one of the sides coating the thermoformed end sheet the composition by the preparation based on amino acids and optionally polysaccharides and other additives and possibly clay.
• thermoformed sheet resulting from the composition is coated on at least one of the faces which will become the inner face of the envelope. Any technique of spray coating, rollers, jet, soaking is possible.
• the amount of coating per m2 is between 100 to 5000 g / m2 and preferably between 1000 to 4000g / m2. At least one of the two sheets is coated in this way, both the sheet called to become the so-called lower sheet, constituting the lower part of the packaging envelope, by a suitable forming, the so-called upper sheet called to become the cover of the packaging envelope.
• the manufacturing method comprises a final step j) of preparing and introducing an aerobic bacterial colony to be packaged in the envelope in the form of a dispersion in a nutrient liquid medium.
• the conditions of introduction of the bacterial colony involve preparation of the aqueous medium of the bacterial colony, pre-introduction of the selected bacterial strains in this aqueous medium and introduction into the conditioning envelope before closure, strains and the medium of the bacterial colony. growth before closure.
• each is filled with an aqueous solution in which is located the bacterial colony.
• the bacterial colony is selected according to the types of use considered, for example for controlled degradation of hydrocarbons, for action on more complex media or other.
• the viscosity of the bacterial solution is chosen so that the introduction is easy and can be. carried out without discontinuity, and that the quantities of liquid loaded with bacterial strains are the most appropriate with respect to the volumes of the packaging envelope, • nutrient availability, characteristics of kinetics of degradation and therefore availability of bacteria required for the final application.
• lipids, proteins, bacteria, yeasts and / or fungi produced sequentially sterile fermenters are premixed and packaged with the mineral salts and trace elements constituting the growth medium.
• Yeasts belong to the family Saccharomycetes, Myxomycetes, Candida. Fungi are used for the degradation of certain lipid compounds.
• the biodegradable product manufacturing method of environmental biotreatment comprises a final step k) closing the casing by a coated sheet and cutting envelopes thus formed.
• the at least two sheets coated with the coating comprising in particular amino acids, formed in the form of cell, to the lower sheet, filled with cell aerobic bacterial colony in aqueous solution, formed in the form of socket or left flat to the . upper cover sheet, are then welded around their periphery to form a sealed packaging envelope, for example a capsule, leaving free the internal parts.
• the welding on the periphery is made a few millimeters wide, by. i hot pressure.
• the packaging envelopes thus produced are cut out and become easily manipulable. They can be stored for long periods of time, without biodegradation, controlled biodegradation only starting when the moisture content of the outdoor environment at
• the envelope packaging of the aerobic bacterial colony s, ccording to the invention is preferably in the form of a tube or profile manufactured by extrusion from the composition extrudable biodegradable 'a) comprising at least one fibrous milled vegetable material, at least one binding agent of vegetable origin or of synthetic origin and at least one agent plasticizer, all the compounds being present qualitatively and quantitatively as previously mentioned.
• the formulated content of the envelope is a formulated fodder placed inside the packaging envelope and composed of a culture containing bacteria, fungi and / or aerobic yeasts mixed with a nutrient substrate containing at least one acid amino, acting as an osmotic membrane and as a nutrient medium for the bacteria contained at the time of their revival and at least one viscosity modifier, all compounds being present qualitatively and quantitatively as previously mentioned.
• the applications for the biodegradable environmental biotreatment product are, for example, the wastewater bioaugmentation treatment in an industrial environment, in situations of overload, accidental toxicity, the treatment of bioaugmentation of urban wastewater, when treatment plants were undersized, or in the case of the treatment of hydrocarbon-contaminated soils, the treatment of fats, the treatment of compounds. volatile organic compounds or the treatment of environmental media in general, and composting.
• the bacterial reviviscence is a function of the relative humidity of the environment, the pH, the temperature, the concentration of dissolved oxygen, the salinity.
• the optimum storage • is obtained at 4 ° C for all strains bacteria, yeasts and / or fungi.
• Heterotrophic strains have a guaranteed half-life of 6 months at a maximum temperature of 14 ° C.
• the sporulating strains have a conservation of several years at the maximum temperature of 25 ° C.
• the fungi depending on the species, can tolerate temperature conditions up to 45 ° C.
• the relative humidity for storage should not exceed 75% at the risk of producing actinomycetes.
• the hydrolysis of the biodegradable environmental biotreatment product is carried out in a few minutes in an aqueous medium and under aerobic conditions at a temperature of between 10 ° C. and 35 ° C.
• the rate of hydrolysis is a function of the thickness of the envelope conditioning and is a guarantee of time control for the operational setting of the strains.
• the mineralization of the casing is effective, depending on the aforementioned conditions parametric , from 4 hours of fermentation.
• Actinomycetes o Actinomycetaceae: Nocardia, Pseudonocardia
• Mushrooms o Mucorales: Rhizopus o Protoascomycetes (yeasts): Candida, Torula,
• EXAMPLE 1 Preparation of the biodegradable product for environmental biotreatment and reviviscence control.
• composition of the envelope comprised 37% dehydrated alfalfa expressed as total weight of the biodegradable composition making it possible to produce the envelope.
• the binder chosen is a turbo-separated wheat flour for which the starch-rich and low-protein fraction has been preserved. This amount was added in a proportion of 34% by weight. Glycerol was added, as well as magnesium stearate and silica, the 100% complement being provided by the addition of water.
• biodegradable composition was carried out under the following conditions: after grinding the dehydrated alfalfa, it was mixed with the wheat flour at the metering hopper of a CLEXTRAL twin-screw extruder BC 45; to this mixture was added mineral additives and magnesium stearate. Once this incorporation of the plant fibrous compound and the vegetable binder (wheat flour) and the processing additives carried out, the incorporation of glycerol and water by injection into a downstream zone of the CLEXTRAL extruder was able to proceed. 'carry out.
• the CLEXTRAL extruder was equipped with an annular coextrusion die allowing the introduction of forage; the wall of the tube thus produced had a thickness ranging from 0.5 to 3 mm; the diameter of the tube made was 1.5 cm.
• I / Clextral extruder had nine zones, zone 1 of conveying, the areas 2 and 3 of mixing, zone 4 provided with a thread-against shear, 5 and 6 heating zones controlled at a temperature of 8O 0 C. at 135 ° C. and preferably stabilized at between 120 ° C. and 130 ° C.
• Zone 7 is a cooling zone distributed over a length corresponding to 3 ducts and for which the temperature has been maintained between 60 ° C. and 80 ° C. .
• composition packaging envelopes according to Table 2 were filled at the time of their manufacture by coextrusion, at the level of the die, with a substrate the
• nutrient consisting of a solution of amino acids whose viscosity had been modified by adding clay and inoculated with a bacterial colony pseudomonas type.
• the formulated feed had the following composition:
• the bacterial concentration was 10 7 to 10 9 cfu / ml.
• the CLEXTRAL extruder was equipped with a feed injection system at the coextrusion die. This system consisted of an extruder feed supply pump, equipped with a jacketed hopper, for cooling the feed to thicken beyond its initial viscosity 'modified .par however the presence of the agent viscosity modifier.
• the coextrusion die was also equipped with a cooling system.
• the extrusion rates were set between 70 kg / h and 80 kg / h, with a single 'coextrusion die; nevertheless, flow rates of 150 to 200 kg / h, with the same extruder but using a double outlet coextrusion die are quite feasible.
• the flow rate of the pump during the tests was about 20 kg / h, while the flow rate of the extruder to achieve the envelope was 50 to 60 kg / h.
• the tube made by means of the biodegradable extrudable filled composition thus obtained was sufficiently flexible to be, once received on a treadmill, converted by flattening and clamping at both ends into a multiplicity of packaging envelopes. containing
• This knife-nipper system included two counter-rotating rollers associated with cutting crowns, making it possible to carry out these operations of forming and cutting / separating the capsules. '
• the capsule was then cooled and dried before being stored and bagged.
• the capsules made during these tests are in the form of small rectangular berlingots, of dimensions 20 to 28 mm in length, 10 to 18 mm in width, 5 to 15 mm in height. These dimensions may change, of the order of centimeters to possibly tens of centimeters, depending on the dies and pinch rollers in place.
• Non-sporulating strains have different storage times that differ from one species to another.
• The. Yeasts (Candida polytica) retain an identical count for a period of 90 days relative to the initial seeding rate. A comparison between the evolution of the count of the bacteria stored in can and those kept in capsule was carried out.
• Packaging envelopes allowed to keep the same level of lipase over the same period.
• packaging envelopes in relation to storage in can, a better preservation of the initial vitality and preservation of the conservation of endo- and exo-enzymes adapted to the treatments envisaged.
• Such a biodegradable environmental biotreatment product offers ease of implementation for the assay since the same supply in liquid form would require the establishment of three metering equipment and flow control.
• a second application consisted of a heavy hydrocarbon treatment for which the effectiveness of the environmental biotreatment product according to the invention was evaluated in a comparative manner.
• the composition of the casing is that from Table 2.
• the composition of the filling is in accordance with Composition 3 of Table 4.
• the environmental biotreatment product according to the invention that is to say in the form of capsules with fodder K) containing the associated bacteria and their nutritive medium (and referenced under the name “Test 1") and Moreover the 'indicator built around' bacteria called “free”, stored in liquid form (and referenced under the name the
• the medium to be cleaned up consisted of a mixture comprising -30 liters of water contaminated with 4 ml of gas oil.
• control to be used for the biological treatment of the same quantities of polluted liquid
• Test 2 Product object of the invention
• test 1 The result of test 1 is the ODT reduction of 76.1% and a reduction of BOD 5 of 75.9%.
• Test 2 is a reduction of the COD of 89.7% . and a reduction of BOD 5 88.8%. .
• the effectiveness of the capsule is greater than that of the bacteria in liquid form added in the same proportions since there is a greater reduction in the BOD and COD in the test 1 than in the test 2.
• FIG. 1 represents the evolution of the chemical oxygen demand (COD) and the 5 day biological oxygen demand (BOD5) for the environmental biotreatment product respectively. according to the invention (Table 6) and for the control implemented (Table 5).
• the environmental biotreatment product of the invention has a much better behavior in COD and BOD5 than the control, tested under the best conditions of concentration of bacteria and nutrient medium present.
• Example 2 Various compositions presented in Table 2) of Example 1 were made from dehydrated alfalfa with 5 levels alfalfa ranging from 20 to to 40% of the total weight of the composition.
• the binder selected is a turbo-split wheat flour for which the starch-rich, low-protein fraction has been preserved.
• each biodegradable composition 1 to 6 was carried out under the following conditions:
• the extrusion was carried out in two phases, by means of a single-screw extruder, a first phase consisting of
• Extrusion head pressures ranged from 100 to 130 bar, while temperatures • were modulated between 90 ° C and 12O 0 C for a speed of rotation of the screw maintained at 50 revolutions / min.
• the sheet thus obtained was sufficiently flexible to be rolled up and transformed into a packaging envelope.
• This packaging casing was coated with an amino acid solution and filled with a pseudomonal bacterial colony.
• the application was intended for composting.
• the mineralization of the casing was complete, for a pH of 6.5 at a temperature of 30 ° C. and a relative humidity of 75%, starting from 6 hours of fermentation.
• Non-sporulating strains have different storage times that differ from one species to another. .0
• the packaging envelopes have kept the same level of lipase over the same period.
• packaging envelopes, 0 allow, compared to the storage in can, a better preservation of the initial vitality and preservation of the conservation of endo- and exo-enzymes adapted to the treatments envisaged.
• Such a biodegradable environmental biotreatment product offers ease of implementation for the assay since the same supply in liquid form would require the establishment of three metering equipment and flow control.
• the kinetics of degradation of the organic matter is greater for the biodegradable product of environmental biotreatment compared to the same mass of liquid bacteria intake, and this because of the preservation facilities offered by the mode of conditioning according to the invention, in particular transfer of oxygen and therefore guarantee of an optimal treatment and superior to the treatment by the simple bacteria kept in a liquid form in can.

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