EP1590089A1 - Procede de formation d'un reseau d'echantillons comprenant une etape de distribution automatique d'un liquide a haute viscosite - Google Patents
Procede de formation d'un reseau d'echantillons comprenant une etape de distribution automatique d'un liquide a haute viscositeInfo
- Publication number
- EP1590089A1 EP1590089A1 EP03789392A EP03789392A EP1590089A1 EP 1590089 A1 EP1590089 A1 EP 1590089A1 EP 03789392 A EP03789392 A EP 03789392A EP 03789392 A EP03789392 A EP 03789392A EP 1590089 A1 EP1590089 A1 EP 1590089A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- array
- making
- dispensing
- samples
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01F—MEASURING VOLUME, VOLUME FLOW, MASS FLOW OR LIQUID LEVEL; METERING BY VOLUME
- G01F11/00—Apparatus requiring external operation adapted at each repeated and identical operation to measure and separate a predetermined volume of fluid or fluent solid material from a supply or container, without regard to weight, and to deliver it
- G01F11/006—Details or accessories
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01F—MEASURING VOLUME, VOLUME FLOW, MASS FLOW OR LIQUID LEVEL; METERING BY VOLUME
- G01F11/00—Apparatus requiring external operation adapted at each repeated and identical operation to measure and separate a predetermined volume of fluid or fluent solid material from a supply or container, without regard to weight, and to deliver it
- G01F11/02—Apparatus requiring external operation adapted at each repeated and identical operation to measure and separate a predetermined volume of fluid or fluent solid material from a supply or container, without regard to weight, and to deliver it with measuring chambers which expand or contract during measurement
- G01F11/021—Apparatus requiring external operation adapted at each repeated and identical operation to measure and separate a predetermined volume of fluid or fluent solid material from a supply or container, without regard to weight, and to deliver it with measuring chambers which expand or contract during measurement of the piston type
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01F—MEASURING VOLUME, VOLUME FLOW, MASS FLOW OR LIQUID LEVEL; METERING BY VOLUME
- G01F11/00—Apparatus requiring external operation adapted at each repeated and identical operation to measure and separate a predetermined volume of fluid or fluent solid material from a supply or container, without regard to weight, and to deliver it
- G01F11/02—Apparatus requiring external operation adapted at each repeated and identical operation to measure and separate a predetermined volume of fluid or fluent solid material from a supply or container, without regard to weight, and to deliver it with measuring chambers which expand or contract during measurement
- G01F11/021—Apparatus requiring external operation adapted at each repeated and identical operation to measure and separate a predetermined volume of fluid or fluent solid material from a supply or container, without regard to weight, and to deliver it with measuring chambers which expand or contract during measurement of the piston type
- G01F11/024—Apparatus requiring external operation adapted at each repeated and identical operation to measure and separate a predetermined volume of fluid or fluent solid material from a supply or container, without regard to weight, and to deliver it with measuring chambers which expand or contract during measurement of the piston type the pistons reciprocating in rotatable cylinders
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01F—MEASURING VOLUME, VOLUME FLOW, MASS FLOW OR LIQUID LEVEL; METERING BY VOLUME
- G01F25/00—Testing or calibration of apparatus for measuring volume, volume flow or liquid level or for metering by volume
- G01F25/0092—Testing or calibration of apparatus for measuring volume, volume flow or liquid level or for metering by volume for metering by volume
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00279—Features relating to reactor vessels
- B01J2219/00281—Individual reactor vessels
- B01J2219/00283—Reactor vessels with top opening
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00279—Features relating to reactor vessels
- B01J2219/00306—Reactor vessels in a multiple arrangement
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00351—Means for dispensing and evacuation of reagents
- B01J2219/00353—Pumps
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00351—Means for dispensing and evacuation of reagents
- B01J2219/00364—Pipettes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00585—Parallel processes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/0068—Means for controlling the apparatus of the process
- B01J2219/00686—Automatic
- B01J2219/00689—Automatic using computers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/0068—Means for controlling the apparatus of the process
- B01J2219/00686—Automatic
- B01J2219/00691—Automatic using robots
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00718—Type of compounds synthesised
- B01J2219/00756—Compositions, e.g. coatings, crystals, formulations
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
- C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N2035/1027—General features of the devices
- G01N2035/1034—Transferring microquantities of liquid
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1002—Reagent dispensers
Definitions
- the invention pertains to a method of making an array of samples comprising the step of automatically dispensing a high-viscosity liquid under dispensing conditions.
- high-viscous are meant solutions, liquids, suspensions, and gelled materials with a viscosity during dispensing of 250 mPa.s or more, preferably measured at the temperature at which the material is dispensed.
- a positive displacement syringe pump is employed.
- US 2002/0159919 describes a method for high-speed precision dispensing of microfluidic quantities of reagents and other liquids with a wide dynamic range of dispense volumes using this type of pump.
- WO 00/66269 discloses a method for sampling fluids in amounts of less than 5 microliters per aliquot to a dispense element array using a positive displacement syringe pump such as a CAVRO syringe pump.
- US 2001/0016631 also mentions the use of a syringe pump or gear pump in combinatorial experimentation. However, this type of pump is not suitable for handling high-viscosity liquids.
- valves of gear-wheeled pumps get stuck in viscous masses having a viscosity higher than 500 mPa.s.
- plunger pumps have similar problems regarding wear, chemical resistance, and valve problems.
- Membrane pumps which are also regularly used for pumping viscous materials, also suffer from valve problems, as do hybrid membrane-plunger pumps.
- peristaltic pumps were found to be suitable to dispense viscous liquids, but their accuracy and therefore their reproducibility is very poor, rendering them unsuitable for application in automated systems.
- the volume of liquid dispensed is significantly lower than the intended volume, and at least cannot reproducibly be dispensed in the vials without contamination of e.g. system liquids.
- the differences in dispensed volumes depend on the rheological nature of complex liquids and the amount of time used for aspiration and dispensing, which make these conventional systems far less suited to automatic dispensing of viscous liquids as for example required for high-throughput experimentation.
- samples cannot be dispensed within the short period of time required in efficient automated high-throughput experimentation.
- the skilled person generally knows the difficulty of automatic handling and administering of viscous materials, see for instance B. Chrisholm et al., Prog. Org. Coat. 2002, 45, 313-321.
- a method of making an array of samples comprising the step of automatically dispensing a liquid with a viscosity under dispensing conditions of at least 250 mPa.s, preferably at least 500 mPa.s, more preferably at least 750 mPa.s, by using a positive displacement rotary piston pump.
- Said viscosity is preferably measured in the conventional way at the temperature at which the material is dispensed. If said temperature is unknown, it may be preferable to measure at a temperature of 23°C.
- the method of the invention is particularly useful in high-throughput experimentation using arrays of samples or reaction vials.
- Such an array preferably comprises at least three vials, and usually substantially more than three.
- the method is suitable for high-viscosity liquids, i.e. having a viscosity of 250 mPa.s or more, even up to 100 Pa.s. Liquids with a higher viscosity can be applied, however, this is done mostly at the cost of accuracy and reproducibility.
- the method of the invention further is suitable for fast dispensing of the high-viscous liquid, and each sample in the array can easily be dispensed at a dispense rate of at least 500 ⁇ l/min, using a pipetting robot system and a robot gripper arm, so that this experimental set-up can prepare arrays of samples.
- the dispense rate is at least 1 ,000 ⁇ l/min, more preferably at least 2,500 ⁇ l/min, and most preferably at least 5,000 ⁇ l/min.
- These arrays of samples can be used in combinatorial experiments. This set-up is automated so that simple programming of the experimentation steps can be carried out, allowing an automated procedure to prepare these arrays of samples.
- the method comprises a step of aspirating the viscous liquid using tubing with a large diameter, at least 5 mm in meter, and preferably dispensing it using a conical dispense tip. This embodiment further adds to the accuracy and reproducibility of the method.
- the automated arm either brings the dispense tip to the array of sample vials, or it moves one or more sample vials to the dispense tip. It further is an advantage for obtaining accurate dosing when the dosing amount is determined gravimetrically. In that case, the vials are placed on a balance, and the pump speed is controlled by a protocol using the target weight and the actual weight as parameters.
- the method of the invention can be operated due to the selection of a suitable pump, i.e. a positive displacement rotary piston pump.
- a suitable pump i.e. a positive displacement rotary piston pump.
- Such pumps appear to be suitable for accurate dispensing of high-viscous liquids in high-throughput experimentation, which dispensing up to now was not possible.
- any positive displacement rotary piston pump can be used, as long as its capacity is sufficient for the purpose for which it is used.
- Preferred positive displacement rotary piston pumps are rotary-axial piston pumps, such as can be obtained at LaboCat (The Netherlands) under the trade names HPLH PCON-CTM, CATTM micrometering pump, and the like.
- the pump may be used in combination with software for flexible control, such as CAT LabControlTM software.
- the parts of the pump are made of materials that are resistant and inert under the conditions used, such as ceramics, PTFE (polytetrafluoroethylene), and stainless steel.
- the tubes used for loading the pump system aspiration tubes
- the tubing for connecting the pump and the dispense tip preferably have an inner diameter larger than 3 mm, more preferably larger than 4 mm, and most preferably larger than 5 mm. Thinner tubing results in a significant loss of reproducibility and an increased dosing time.
- the material used for the dispense tubing should preferably be relatively rigid, so the internal pressure in the tubing cannot be compensated for by a volume increase caused by the elasticity of the tubing.
- Preferred material for making the tube is PTFE such as Teflon®, which is preferred over silicone rubber tubing.
- Run 1 Tubing used: about 10 cm Teflon aspirating tube with 6 mm diameter and 30 cm silicone dispensing tube with 6 mm diameter.
- Run 2 Same as run 1 , but a dosing tip with 1 mm diameter was mounted at the end of the dispensing tube.
- Example 2 This example was performed as Example 1 , with the following constituents.
- Viscous liquid Resin type Setalux ® 6100-GR-74 (ex Akzo Nobel Resins)
- Viscosity 20 Pa.s (23°C, 100 s "1 )
- Run 1 Tubing used: about 10 cm Teflon aspirating tube with 6 mm diameter.
- Run 2 Same as run 1 , but the dispensing speed was lowered to 1 ml/min, and a tip with a diameter ⁇ 1 mm was mounted on the dispensing tube.
- Example 2 This example was performed as Example 1 , except in combination with a Lipos (Zinsser AnalyticTM) automated liquid and powder dispensing system, which consists of a robot gripper for picking up vials and trays of vials to make a series of samples.
- a Lipos (Zinsser AnalyticTM) automated liquid and powder dispensing system which consists of a robot gripper for picking up vials and trays of vials to make a series of samples.
- Viscous liquid Resin type: Setal ® 90173-SS-50 (ex Akzo Nobel Resins) Viscosity: 20 Pa.s (23°C, 1 s "1 ) or 0.80 Pa.s (23°C, 100 s "1 )
- Viscosity 20 Pa.s (23°C, 1 s "1 ) or 0.80 Pa.s (23°C, 100 s "1 )
- Dispensing rate 20 ml/min volume 20 ml
- Example 4 From Example 3 it can be seen that when tubing with a large diameter is used, and the viscous liquid is dispensed into a conical dispense tip, excellent accuracy and reproducibility are achieved even at high dispensing rates.
- Example 4
- a dispensing rate of 2,000 ⁇ l/min was used for set volumes up to 1 ,500 ⁇ l.
- a dispensing rate of 8,000 ⁇ l/min was used for higher set volumes.
- Viscous liquid resin type Setalux 1200-XX-55 (ex Akzo Nobel Resins)
- Viscosity 1.6 Pa.s (100 s " ⁇ 23°C).
- Example 3 This example was performed as Example 3, dosing a viscous gel. Viscous gel: 2 wt.% xanthane. From Table V it can be derived that even when dosing a viscous gel, accurate and reproducible results are obtained.
Landscapes
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Fluid Mechanics (AREA)
- General Physics & Mathematics (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Feeding, Discharge, Calcimining, Fusing, And Gas-Generation Devices (AREA)
Abstract
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP03789392A EP1590089A1 (fr) | 2002-12-30 | 2003-12-19 | Procede de formation d'un reseau d'echantillons comprenant une etape de distribution automatique d'un liquide a haute viscosite |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP02080678 | 2002-12-30 | ||
EP02080678 | 2002-12-30 | ||
PCT/EP2003/014781 WO2004058407A1 (fr) | 2002-12-30 | 2003-12-19 | Procede de formation d'un reseau d'echantillons comprenant une etape de distribution automatique d'un liquide a haute viscosite |
EP03789392A EP1590089A1 (fr) | 2002-12-30 | 2003-12-19 | Procede de formation d'un reseau d'echantillons comprenant une etape de distribution automatique d'un liquide a haute viscosite |
Publications (1)
Publication Number | Publication Date |
---|---|
EP1590089A1 true EP1590089A1 (fr) | 2005-11-02 |
Family
ID=32668883
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP03789392A Withdrawn EP1590089A1 (fr) | 2002-12-30 | 2003-12-19 | Procede de formation d'un reseau d'echantillons comprenant une etape de distribution automatique d'un liquide a haute viscosite |
Country Status (3)
Country | Link |
---|---|
EP (1) | EP1590089A1 (fr) |
AU (1) | AU2003293979A1 (fr) |
WO (1) | WO2004058407A1 (fr) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE102007019186B4 (de) * | 2007-04-20 | 2011-11-17 | Qiagen Instruments Ag | Pipettiergerät und Verfahren für dem Betrieb des Pipettiergerätes |
CN112880762B (zh) * | 2021-02-19 | 2021-09-24 | 四川省水文水资源勘测局 | 一种干旱地区引水绿化流量测量方法 |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5958342A (en) * | 1996-05-17 | 1999-09-28 | Incyte Pharmaceuticals, Inc. | Jet droplet device |
US5996650A (en) * | 1996-11-15 | 1999-12-07 | Oden Corporation | Net mass liquid filler |
US20020159919A1 (en) * | 1998-01-09 | 2002-10-31 | Carl Churchill | Method and apparatus for high-speed microfluidic dispensing using text file control |
US6485692B1 (en) * | 1998-12-04 | 2002-11-26 | Symyx Technologies, Inc. | Continuous feed parallel reactor |
EP1181100A4 (fr) * | 1999-05-03 | 2002-09-25 | Ljl Biosystems Inc | Systeme integre de traitement d'echantillons |
US6386396B1 (en) * | 2001-01-31 | 2002-05-14 | Hewlett-Packard Company | Mixing rotary positive displacement pump for micro dispensing |
-
2003
- 2003-12-19 WO PCT/EP2003/014781 patent/WO2004058407A1/fr not_active Application Discontinuation
- 2003-12-19 EP EP03789392A patent/EP1590089A1/fr not_active Withdrawn
- 2003-12-19 AU AU2003293979A patent/AU2003293979A1/en not_active Abandoned
Non-Patent Citations (1)
Title |
---|
See references of WO2004058407A1 * |
Also Published As
Publication number | Publication date |
---|---|
AU2003293979A1 (en) | 2004-07-22 |
WO2004058407A1 (fr) | 2004-07-15 |
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