EP1587951A4 - Substrate detection assay - Google Patents

Substrate detection assay

Info

Publication number
EP1587951A4
EP1587951A4 EP04703016A EP04703016A EP1587951A4 EP 1587951 A4 EP1587951 A4 EP 1587951A4 EP 04703016 A EP04703016 A EP 04703016A EP 04703016 A EP04703016 A EP 04703016A EP 1587951 A4 EP1587951 A4 EP 1587951A4
Authority
EP
European Patent Office
Prior art keywords
detection assay
substrate detection
substrate
assay
detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP04703016A
Other languages
German (de)
French (fr)
Other versions
EP1587951A2 (en
Inventor
Andrew Napper
Jeffrey Hixon
Tom Mcdonagh
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Elixir Pharmaceuticals Inc
Original Assignee
Elixir Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Elixir Pharmaceuticals Inc filed Critical Elixir Pharmaceuticals Inc
Publication of EP1587951A2 publication Critical patent/EP1587951A2/en
Publication of EP1587951A4 publication Critical patent/EP1587951A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G01N33/5735—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes co-enzymes or co-factors, e.g. NAD, ATP
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP04703016A 2003-01-16 2004-01-16 Substrate detection assay Withdrawn EP1587951A4 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US44072303P 2003-01-16 2003-01-16
US440723P 2003-01-16
PCT/US2004/001239 WO2004064739A2 (en) 2003-01-16 2004-01-16 Substrate detection assay

Publications (2)

Publication Number Publication Date
EP1587951A2 EP1587951A2 (en) 2005-10-26
EP1587951A4 true EP1587951A4 (en) 2007-04-25

Family

ID=32771853

Family Applications (1)

Application Number Title Priority Date Filing Date
EP04703016A Withdrawn EP1587951A4 (en) 2003-01-16 2004-01-16 Substrate detection assay

Country Status (4)

Country Link
EP (1) EP1587951A4 (en)
AU (1) AU2004206887A1 (en)
CA (1) CA2513184A1 (en)
WO (1) WO2004064739A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070078100A1 (en) * 2003-10-14 2007-04-05 The Board Of Trustees Of The University Of Illinoi Methods of detecting poly(adp-ribose) polymerase and other nad+ utilizing enzymes
WO2008100376A2 (en) * 2007-02-15 2008-08-21 Sirtris Pharmaceuticals, Inc. Truncation variants of sirt1 and methods of use thereof
EP2446048A4 (en) * 2009-06-23 2013-04-03 Harvard College Methods and kits for measuring enzyme activity

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4704365A (en) * 1986-02-24 1987-11-03 Abbott Laboratories Composition and method for stabilization of dinucleotides

Non-Patent Citations (11)

* Cited by examiner, † Cited by third party
Title
ALVAREZ-GONZALEZ R ET AL: "A NEW HIGHLY SELECTIVE PHYSICOCHEMICAL ASSAY TO MEASURE NAD-+ IN INTACT CELLS", ANALYTICAL BIOCHEMISTRY, vol. 156, no. 2, 1986, pages 473 - 480, XP008070288, ISSN: 0003-2697 *
ALVAREZ-GONZALEZ R ET AL: "EVALUATION OF IMMOBILIZED BORONATES FOR STUDIES OF ADENINE AND PYRIDINE NUCLEOTIDE METABOLISM", ANALYTICAL BIOCHEMISTRY, vol. 135, no. 1, 1983, pages 69 - 77, XP008070097, ISSN: 0003-2697 *
BARLOW ANDREW L ET AL: "dSIR2 and dHDAC6: Two novel, inhibitor-resistant deacetylases in Drosophila melanogaster", EXPERIMENTAL CELL RESEARCH, vol. 265, no. 1, 15 April 2001 (2001-04-15), pages 90 - 103, XP002404082, ISSN: 0014-4827 *
BEDALOV ANTONIO ET AL: "Identification of a small molecule inhibitor of Sir2p", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE, WASHINGTON, DC, US, vol. 98, no. 26, 18 December 2001 (2001-12-18), pages 15113 - 15118, XP002350537, ISSN: 0027-8424 *
BOURIOTIS V ET AL: "APPLICATIONS OF IMMOBILIZED PHENYL BORONIC ACIDS AS SUPPORTS FOR GROUP SPECIFIC LIGANDS IN THE AFFINITY CHROMATOGRAPHY OF ENZYMES", JOURNAL OF CHROMATOGRAPHY, vol. 210, no. 2, 1981, pages 267 - 278, XP002404084 *
ÇAMLI S T ET AL: "Nucleotide isolation by boronic acid functionalized hydrophilic supports", COLLOIDS AND SURFACES A: PHYSICOCHEMICAL AND ENGINEERING ASPECTS 30 JUL 2002 NETHERLANDS, vol. 207, no. 1-3, 30 July 2002 (2002-07-30), pages 127 - 137, XP002404081, ISSN: 0927-7757 *
COX R: "NICOTINAMIDE METHYL TRANSFERASE INHIBITION BY S ADENOSYL ETHIONINE", CANCER LETTERS, vol. 17, no. 3, 1983, pages 295 - 300, XP002404079, ISSN: 0304-3835 *
LANDRY J ET AL: "Role of NAD+ in the deacetylase activity of the SIR2-like proteins", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 278, no. 3, 30 November 2000 (2000-11-30), pages 685 - 690, XP002963942, ISSN: 0006-291X *
MCDONAGH ET AL: "Microplate filtration assay for nicotinamide release from NAD using a boronic acid resin", METHODS : A COMPANION TO METHODS IN ENZYMOLOGY, ACADEMIC PRESS INC., NEW YORK, NY, US, vol. 36, no. 4, August 2005 (2005-08-01), pages 346 - 350, XP005012565, ISSN: 1046-2023 *
SENEL S ET AL: "Nucleotide adsorption-desorption behaviour of boronic acid functionalized uniform-porous particles", JOURNAL OF CHROMATOGRAPHY B, vol. 769, no. 2, 5 April 2002 (2002-04-05), pages 283 - 295, XP002404083, ISSN: 0378-4347 *
ZAYATS M ET AL: "Electrical contacting of flavoenzymes and NAD(P)+-dependent enzymes by reconstitution and affinity interactions on phenylboronic acid monolayers associated with Au-electrodes", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMERICAN CHEMICAL SOCIETY, WASHINGTON, DC, US, vol. 124, no. 49, 11 December 2002 (2002-12-11), pages 14724 - 14735, XP002240209, ISSN: 0002-7863 *

Also Published As

Publication number Publication date
WO2004064739A3 (en) 2005-04-21
AU2004206887A1 (en) 2004-08-05
CA2513184A1 (en) 2004-08-05
WO2004064739A2 (en) 2004-08-05
EP1587951A2 (en) 2005-10-26

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Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

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17P Request for examination filed

Effective date: 20050811

AK Designated contracting states

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Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR

AX Request for extension of the european patent

Extension state: AL LT LV MK

RIN1 Information on inventor provided before grant (corrected)

Inventor name: MCDONAGH, TOM

Inventor name: HIXON, JEFFREY

Inventor name: NAPPER, ANDREW

DAX Request for extension of the european patent (deleted)
A4 Supplementary search report drawn up and despatched

Effective date: 20070326

RIC1 Information provided on ipc code assigned before grant

Ipc: G01N 33/53 20060101ALI20070316BHEP

Ipc: G01N 33/573 20060101ALI20070316BHEP

Ipc: C12Q 1/34 20060101AFI20050429BHEP

STAA Information on the status of an ep patent application or granted ep patent

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Effective date: 20070623