EP1581797A4 - Methods for the detection, analysis and isolation of nascent proteins - Google Patents

Methods for the detection, analysis and isolation of nascent proteins

Info

Publication number
EP1581797A4
EP1581797A4 EP04701238A EP04701238A EP1581797A4 EP 1581797 A4 EP1581797 A4 EP 1581797A4 EP 04701238 A EP04701238 A EP 04701238A EP 04701238 A EP04701238 A EP 04701238A EP 1581797 A4 EP1581797 A4 EP 1581797A4
Authority
EP
European Patent Office
Prior art keywords
nascent proteins
analysis
detection
isolation
markers
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP04701238A
Other languages
German (de)
French (fr)
Other versions
EP1581797B1 (en
EP1581797A2 (en
Inventor
Kenneth Rothschild
Sadanand Gite
Jerzy Olejnik
Mark Lim
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ambergen Inc
Original Assignee
Ambergen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ambergen Inc filed Critical Ambergen Inc
Publication of EP1581797A2 publication Critical patent/EP1581797A2/en
Publication of EP1581797A4 publication Critical patent/EP1581797A4/en
Application granted granted Critical
Publication of EP1581797B1 publication Critical patent/EP1581797B1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/82—Translation products from oncogenes

Abstract

This invention relates to non-radioactive markers that facilitate the detection and analysis of nascent proteins translated within cellular or cell-free translation systems. Nascent proteins containing these markers can be rapidly and efficiently detected, isolated and analyzed without the handling and disposal problems associated with radioactive reagents. Preferred markers are dipyrrometheneboron difluoride(4,4-difluoro-4-bora-3a,4a-diaza-s-indacene)dyes.
EP04701238A 2003-01-10 2004-01-09 Methods for the detection, analysis and isolation of nascent proteins Expired - Lifetime EP1581797B1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US339712 2003-01-10
US10/339,712 US7312060B2 (en) 1999-08-25 2003-01-10 Methods for the detection, analysis and isolation of nascent proteins
PCT/US2004/000528 WO2004063714A2 (en) 2003-01-10 2004-01-09 Methods for the detection, analysis and isolation of nascent proteins

Publications (3)

Publication Number Publication Date
EP1581797A2 EP1581797A2 (en) 2005-10-05
EP1581797A4 true EP1581797A4 (en) 2006-08-16
EP1581797B1 EP1581797B1 (en) 2009-09-23

Family

ID=32711151

Family Applications (1)

Application Number Title Priority Date Filing Date
EP04701238A Expired - Lifetime EP1581797B1 (en) 2003-01-10 2004-01-09 Methods for the detection, analysis and isolation of nascent proteins

Country Status (6)

Country Link
US (1) US7312060B2 (en)
EP (1) EP1581797B1 (en)
AT (1) ATE443711T1 (en)
CA (1) CA2512552A1 (en)
DE (1) DE602004023272D1 (en)
WO (1) WO2004063714A2 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1433858A1 (en) * 2002-12-27 2004-06-30 Rengo Co., Ltd. Preparation method of insect cell extract solution for cell-free protein synthesis, the insect cell extract solution and cell-free synthesis method of protein using the insect cell extract solution
US7807407B2 (en) * 2004-07-30 2010-10-05 Ambergen, Inc. Detection of truncation mutations by mass spectrometry
US8114587B2 (en) * 2005-06-23 2012-02-14 Ambergen, Inc. Methods for the detection of colorectal cancer
US20110045991A1 (en) * 2005-06-23 2011-02-24 Sadanand Gite Methods for the Detection of Colorectal Cancer
US20090081669A1 (en) * 2006-02-01 2009-03-26 Encode Bio, Inc. Fluorescent Assays Using Orthogonal tRNA - Aminoacyl Synthetase Pairs
US8906700B2 (en) 2007-11-06 2014-12-09 Ambergen, Inc. Methods and compositions for phototransfer
US8932879B2 (en) * 2007-11-06 2015-01-13 Ambergen, Inc. Methods and compounds for phototransfer
WO2010011281A2 (en) * 2008-07-22 2010-01-28 The Research Foundation Of State University Of New York Methods and compositions for the diagnosis and treatment of cancer
US8609421B2 (en) * 2009-06-12 2013-12-17 Pacific Biosciences Of California, Inc. Single-molecule real-time analysis of protein synthesis
WO2011011669A1 (en) * 2009-07-23 2011-01-27 University Of Wyoming Methods and compositions for detection of biological materials using microfluidic devices
AU2011305249B2 (en) 2010-09-26 2015-07-23 Da Yu Enterprises, L.L.C. Method of recombinant macromolecular production
US9163290B2 (en) * 2012-08-19 2015-10-20 National Health Research Institutes Methods for detecting hepatitis B virus surface gene non-sense mutations
EP2976622B1 (en) * 2013-03-14 2019-11-27 Bio-Rad Laboratories, Inc. Protein standard
US11402376B2 (en) 2018-03-23 2022-08-02 University Of Wyoming Methods and devices for detection of biological materials using electric field assisted rapid analyte capture

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001014578A1 (en) * 1999-08-25 2001-03-01 Ambergen, Inc. Methods for the detection, analysis and isolation of nascent proteins

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6306628B1 (en) * 1999-08-25 2001-10-23 Ambergen, Incorporated Methods for the detection, analysis and isolation of Nascent proteins
US7534861B2 (en) * 2003-01-10 2009-05-19 Ambergen, Inc. Compositions and methods for immunoaffinity purification
US20050277127A1 (en) * 2003-11-26 2005-12-15 Epitomics, Inc. High-throughput method of DNA immunogen preparation and immunization

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001014578A1 (en) * 1999-08-25 2001-03-01 Ambergen, Inc. Methods for the detection, analysis and isolation of nascent proteins

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DEN DUNNEN JOHAN T ET AL: "The protein truncation test: A review.", HUMAN MUTATION, vol. 14, no. 2, 1999, pages 95 - 102, XP009006085, ISSN: 1059-7794 *
GITE S ET AL: "A high-throughput nonisotopic protein truncation test", NATURE BIOTECHNOLOGY, NATURE PUB. CO, NEW YORK, NY, US, vol. 21, no. 2, 13 January 2003 (2003-01-13), pages 194 - 197, XP002993671, ISSN: 1087-0156 *
KAHMANN SABINE ET AL: "A non-radioactive protein truncation test for the sensitive detection of all stop and frameshift mutations.", HUMAN MUTATION, vol. 19, no. 2, 2002, 2002, pages 165 - 172, XP009005892, ISSN: 1059-7794 *
KIRCHGESSER M ET AL: "OPTIMIZED NON-RADIOACTIVE PROTEIN TRUNCATION TEST FOR MUTATION ANALYSIS OF THE ADENOMATOUS POLYPOSIS COLI (APC) GENE", CLINICAL CHEMISTRY AND LABORATORY MEDICINE, WALTER DE GRUYTER UND CO, DE, vol. 36, no. 8, August 1998 (1998-08-01), pages 567 - 570, XP009006524, ISSN: 1434-6621 *
ROWAN ANDREW J ET AL: "Introduction of a myc reporter tag to improve the quality of mutation detection using the protein truncation test.", HUMAN MUTATION, vol. 9, no. 2, 1997, pages 172 - 176, XP009006084, ISSN: 1059-7794 *

Also Published As

Publication number Publication date
DE602004023272D1 (en) 2009-11-05
WO2004063714A3 (en) 2006-03-30
WO2004063714A2 (en) 2004-07-29
EP1581797B1 (en) 2009-09-23
CA2512552A1 (en) 2004-07-29
ATE443711T1 (en) 2009-10-15
EP1581797A2 (en) 2005-10-05
US20040014071A1 (en) 2004-01-22
US7312060B2 (en) 2007-12-25

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