EP1482922A1 - Agents for corneal or intrastromal administration to treat or prevent disorders of the eye - Google Patents

Agents for corneal or intrastromal administration to treat or prevent disorders of the eye

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Publication number
EP1482922A1
EP1482922A1 EP03711552A EP03711552A EP1482922A1 EP 1482922 A1 EP1482922 A1 EP 1482922A1 EP 03711552 A EP03711552 A EP 03711552A EP 03711552 A EP03711552 A EP 03711552A EP 1482922 A1 EP1482922 A1 EP 1482922A1
Authority
EP
European Patent Office
Prior art keywords
urea
corneal
cornea
eye
agent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP03711552A
Other languages
German (de)
French (fr)
Other versions
EP1482922A4 (en
Inventor
Vicken Karageozian
David Castillejos
John Park
Gabriel Arthuro Carpio Aragon
Jose Luis Gutierres Flores
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Vitreo Retinal Technologies Inc
Original Assignee
Vitreo Retinal Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vitreo Retinal Technologies Inc filed Critical Vitreo Retinal Technologies Inc
Publication of EP1482922A1 publication Critical patent/EP1482922A1/en
Publication of EP1482922A4 publication Critical patent/EP1482922A4/en
Withdrawn legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/16—Amides, e.g. hydroxamic acids
    • A61K31/17—Amides, e.g. hydroxamic acids having the group >N—C(O)—N< or >N—C(S)—N<, e.g. urea, thiourea, carmustine
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/40—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with one nitrogen as the only ring hetero atom, e.g. sulpiride, succinimide, tolmetin, buflomedil
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
    • A61K31/513—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim having oxo groups directly attached to the heterocyclic ring, e.g. cytosine
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
    • A61K31/519—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim ortho- or peri-condensed with heterocyclic rings
    • A61K31/52—Purines, e.g. adenine
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
    • A61K31/7052—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides
    • A61K31/706—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom
    • A61K31/7064—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom containing condensed or non-condensed pyrimidines
    • A61K31/7076—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom containing condensed or non-condensed pyrimidines containing purines, e.g. adenosine, adenylic acid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P27/00—Drugs for disorders of the senses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P27/00—Drugs for disorders of the senses
    • A61P27/02—Ophthalmic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P27/00—Drugs for disorders of the senses
    • A61P27/02—Ophthalmic agents
    • A61P27/06—Antiglaucoma agents or miotics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P27/00—Drugs for disorders of the senses
    • A61P27/02—Ophthalmic agents
    • A61P27/10—Ophthalmic agents for accommodation disorders, e.g. myopia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system

Definitions

  • the present invention relates generally to pharmaceutical preparations and medical treatment methods, and more particularly agents (i e Urea, Urea derivatives, non-steroidal anti-inflammatory drugs and Anti-metabolite drugs) used alone or in combinations with each other (or with other agents) to treat or prevent certain disorders of the eye
  • agents i e Urea, Urea derivatives, non-steroidal anti-inflammatory drugs and Anti-metabolite drugs
  • the cornea is the first and most powerful refracting surface of the optical system of the eye Production of a sharp image at the retinal receptors requires that the cornea be transparent and of the appropriate refractive power
  • the average corneal thickness of a normal cornea is 0 56 mm in persons under 25 years of age, this thickness slowly increases with age to become 0 57 in persons over 65 years of age
  • the cornea is somewhat thicker in the periphery than the center
  • the thickness of the cornea is greatest after the eyes have been closed for some time, as after sleeping, this thickness decreases slightly when the eyes are opened and exposed to the dehydrating effects of the air
  • the cornea is composed of six layers a) Epithelium b) Basement membrane c) Bowman's membrane d) Stroma e) Descemet's membrane f) Endothe um a) Epithelium
  • the epithelium consists of 5-6 layers of cells The most superficial cells are flat overlapping squamous cells The middle layer consists of cells that become more columnar as the deeper layers are approached The innermost layer (basal) is made up of columnar cells packed closely together All the cells are held together by a cement substance Also, the cell surfaces form processes that are fitted into corresponding indentations of adjacent cells and connected in places by attachment bodies called desmoso es The basal cells are connected to the basement membrane by hemidesmosomes The epithelium represents 10% of the total wet weight of the cornea Water in the epithelium represents 70% of the wet weight
  • the epithelium consists of 5-6 layers of cells, the healthy epithelium is very strongly attached to each other by desmosomes as well as to the Basement membrane by hemidesmosomes b) Basement membrane: Between the columnar epithelial cells and Bowman's membrane is a basement membrane from 60 - 65 nm thick The basement membrane has been examined histochemically and found to be similar to other basement membranes c) Bowman's membrane: Bowman's layer is a sheet of transparent tissue about 12 ⁇ m thick, without structure as seen by light microscopy Under electron microscopy it appears to be made up of uniform fibrils, probably of collagenous material, running parallel to the surface Bowman's layer possesses little resistance to any pathologic process, and is easily destroyed and never regenerates d) Stroma: The Stroma comprises about 90% of the whole cornea The Stroma is composed of layers of lamellae, each of which runs the full length of the cornea, although the bundles interlace with one another, they are nearly parallel to the surface The cell bodies, called keratocytes
  • the glucosammoglycans represent 4% to 4 5% of the dry weight of the cornea GAG are localized in the interfib ⁇ llar or interstitial space, probably attached to the collagen fibrils or to soluble proteins of the cornea
  • the GAG plays a role in corneal hydration through interaction with electrolytes and water
  • Three major GAG fractions are found in the corneal Stroma keratin sulfate (50%), chondroitin (25%), and chondroitin sulfate A (25%) GAG s have been implicated in the maintenance of the corneal hydration level and transparency e)
  • Descemet's Membrane Is made of type IV collagen, unlike the corneal Stroma, there are no significant amounts of sulfated GAG in the Descemet's membrane The collagen in this membrane is insoluble except in strong alkali or acid and is more resistant to collagenase than corneal stroma collagen Jakus 2 has observed with the electron microscope that this membrane
  • Descemet's membrane Its inner surface is bathed by the aqueous humor
  • endothelium cell layer has limited, if any, reproductive capacity Aging causes cell loss, and the remaining cells enlarge and spread so that Descemet's membrane remains completely covered therefore endothelial cell density, expressed as cells per unit area, decreases with age Similarly, cell loss from trauma, inflammation, or surgery is compensated for by increased cell size and decreased cell density
  • Corneal metabolism embraces a series of chemical processes by which energy is obtained and utilized for the normal functions of the cornea In the cornea, energy is needed for maintenance of its transparency and dehydration Energy in the form of ATP is generated by the breakdown of glucose into lactic acid and into carbon dioxide and water (i e , Krebs Cycle) The cornea obtains glucose mainly from the aqueous humor
  • the tears and hmbal capillaries appear to contribute minimal amounts of glucose and Oxygen for corneal metabolism
  • the oxygen consumption of the epithelium and endothehum can be approximately 26 times that of the stroma
  • the corneal endothelium gets most of its required oxygen from the aqueous humor, while the corneal epithelium gets much of its oxygen from either the capillaries at the hmbus or from the oxygen dissolved in the pre-corneal film
  • Radial keratotomy is a surgical procedure to improve myopia by changing the corneal curvature This is achieved by making several deep incisions in the cornea in a radial pattern
  • the eye surgeon makes 4, 8, or 16 incisions so as to flatten the curvature of the central cornea, thus correcting the patient's vision
  • the main drawbacks of RK include, a) It can only be used to correct low levels of myopia b) This surgical procedure cannot correct hyperopia c) RK procedure seriously weakens the cornea and creates corneal scars d)
  • the corneal curvature changes are temporary and frequently continue to change with time
  • Photorefractive keratectomy is a surgical procedure that uses the excimer laser, which is controlled by a computer With the PRK procedure, the excimer laser ablates and sculpts the corneal surface to the desired shape to correct the patient's vision
  • PRK is a surgical procedure, it can result in complications Infection is the most serious complication resulting from the ablation of a large area of the corneal epithelium
  • delayed corneal healing because of the absence of the corneal epithelium corneal haze, corneal scarring, over correction or under-correction and development of astigmatism are other complications of PRK These complications must be treated with medications or further surgery
  • Laser in-situ keratomileusis 11 is a surgical procedure that is a variation on PRK involving an excimer laser and a precise cutting tool called a microkeratome
  • the microkeratome is used to make a 150-175 micron circular flap of the cornea
  • the circular flap is flipped back, as if on a hinge, to expose the stromal layer of the cornea With the flap folded back, the refractive eye surgeon now ablates the stroma and makes the refractive correction using the excimer laser
  • the circular corneal flap is repositioned on the ablated cornea to complete the procedure With a precision laser treatment and normal reattachment and healing of the corneal flap the refractive results of good vision correction are very rapid There is, however, a significant list of potential complications and risks associated with LASIK procedure, failure of the microkeratome to leave a hinge on the corneal flap with the first incision, loss of the corneal flap after the operation, slipping of the flap and healing off center, first incision is too deep or
  • Laser epithelial keratomileusis is a surgical procedure that is a variation on PRK involving an excimer laser that combines the advantages and eliminates the disadvantages of PRK and LASIK
  • a 7 0 mm circular area of the epithelium is marked with a Hoffer trephine centered over the pupil
  • the corneal epithelium is removed by using a blunt spatula, or is exposed to 20% isopropyl alcohol solution which allows the corneal epithelium to be peeled off
  • the surgeon ablates and sculpts the corneal surface to the desired shape to correct the patient's vision
  • the corneal epithelial flap created by the alcoholic solution is placed back onto the ablated cornea, a drop of antibiotic, a drop of non-steroidal anti-inflammatory agent and a therapeutic contact lens is applied to the corrected eye
  • Orthokeratology is a non-surgical procedure designed to correct refractive errors by reshaping the cornea to the corneal curvature required to achieve emmetropia This is accomplished by applying a series of hard contact lenses that change the corneal curvature until the desired curvature is achieved However once the desired curvature has been produced, retainer hard contact lenses must be worn to stabilize the results otherwise regression will occur
  • Enzyme Orthokeratology is related to traditional Orthokeratology in that it is defined primarily as a contact lens procedure of correcting refractive errors of the eye by reshaping the cornea to the curvature required for emmetropia
  • the system is enhanced by enzymatically softening the cornea, and reshaping is obtained in a shorter period of time, and retainer lenses may not be required for good visual acuity after removal of the contact lens from the eye and regression will not be a problem
  • Chemical Orthokeratology is related to traditional Orthokeratology in that it is defined primarily as a contact lens procedure of correcting refractive errors of the eye by reshaping the cornea to the curvature required for emmetropia
  • the system is enhanced by applying topically or by intra-stromal injection a chemical that is not an enzyme and softening the cornea, and reshaping is obtained in a shorter period of time, and retainer lenses may not be required for good visual acuity after removal of the contact lens from the eye and regression will not be a problem SUMMARY OF THE INVENTION
  • the present invention provides methods for treating or preventing disorders of the eye of a human or veterinary patient by administering topically onto the eye or by injection into the eye (e g intravitreal, intrastromal or sub-conjunctival injection) a therapeutically effective amount of an aqueous solution containing an agent selected from urea, a urea derivative, non-enzymatic protein urea, non-enzymatic proteins, nucleo
  • the agent may be administered in combination with an antimetabolite compound such as, mitomicyn, methotrexate, thiourea, hydroxyurea, 6-mercaptopur ⁇ ne, thioguanine, 5-fluorourac ⁇ l, cytosine arabinoside and 5-azacyt ⁇ d ⁇ ne
  • an antimetabolite compound such as, mitomicyn, methotrexate, thiourea, hydroxyurea, 6-mercaptopur ⁇ ne, thioguanine, 5-fluorourac ⁇ l, cytosine arabinoside and 5-azacyt ⁇ d ⁇ ne
  • the agent may be administered in combination with an antmeoplastic agent such as Actinomycin D, daunorubicin, doxorubicin, idarubicin, bleomycins, or phcamycin may also be used in combination with these anti-metabolites
  • corneal epithelium As explained above, the corneal epithelial cells are held together by a cement substance In addition the surfaces of the cells form processes that are fitted into the corresponding indentations of the adjacent cells and connected by attachment bodies called desmosomes In addition the basal cells of the epithelium are connected to the basement membrane by hemidesmosomes When the corneal epithelium is damaged by chemical or physical means, swelling of the stroma follows Abrasion of the cornea or any condition leading to the loss of epithelium is likely to produce localized areas of corneal swelling and cloudiness and allows microbial access and bacterial infections Fortunately, the corneal epithelium regenerates rapidly, and the excessive hydration and wound closure in the absence of bacterial infections is slight and transient
  • the present invention also provides methods for enhancing healing of the cornea after LASEK surgery
  • an agent selected from urea, a urea derivative, non-enzymatic protein urea, non-enzymatic proteins, nucleosides, nucleotides and their derivatives e g , adenine, adenosine, cytosine, cytadine, guanine, guanitadine, guanidine, guanidinium chloride, guanidinium salts, thymidine, thymitadme uradine, uracil, cysteme
  • reduced thioctic acid uric acid, calcium acetyl sahcylate, ammonium sulfate or other compounds capable of causing non enzymatic dissolution of the proteoglycans or any possible combination thereof, is applied top
  • an agent of the present invention e g , 0 01 % - 20 0% aqueous urea solution
  • a few drops of an agent of the present invention is placed onto the surface of the excimer laser ablated stroma before the microkeratome cut flap of the cornea is repositioned on the laser ablated cornea
  • the urea solution placed at the interface of the two corneal flaps will result in the localized solubilization the stromal proteoglycans and eliminate the interface gap, thus producing optimum vision correction
  • the localized solubilization of the proteoglycans of the stroma will result in the compression of the collagen fibril packing for better visual performance, but normal transparency
  • the present invention also provides methods for softening the cornea by administering to the cornea an agent selected from urea, a urea derivative, nonenzymatic protein urea, non-enzymatic proteins, nucleosides, nucleotides and their derivatives (e g , adenine, adenosine, cytosine, cytadine, guanine, guanitadine, guanidine, guanidinium chloride, guanidinium salts, thymidine, thymitadme, uradine, uracil cysteme) reduced thioctic acid, uric acid, calcium acetyl sahcylate, ammonium sulfate or other compounds capable of causing non enzymatic dissolution of
  • the shape of the cornea is based on the collagen fibrils of the stroma which are held in place at a much specified distance from each other in parallel along with the mucopolysacchandes cement layers between these collagen fibrils
  • the Urea and Urea derivatives have the ability of solubi zing the mucopolysacchandes as well as various proteins
  • the stroma is therefore softened and becomes more pliable and easy to mold to a more desirable shape
  • the cornea softening agent comprises urea or a urea derivative together with pharmaceutically acceptable carriers and additives
  • the preparation may be supplied in a liquid or lyophilized form
  • the cornea softening agent in accordance with the present invention is administered to the cornea in a number of ways Typically, the agent is administered either directly in the form of eye drops, or by the use of a corneal softening agent delivery vehicle, which may include special drug delivery systems including hposomes, sustained release gels and implantable solid dosage forms as well as contact lens and biodegradable corneal collagen shield Non-Surgical Treatment and Elimination of Corneal Haze and Corneal Opacification
  • a reduction of visual acuity and blindness may result from a lack of corneal clarity caused by corneal traumas, corneal scars, or any other cause of corneal opacification
  • Patients who have a reduction of visual acuity as a result of corneal opacities are estimated to be three million
  • the current treatment for corneal opacity is corneal transplantation using a surgical procedure called penetrating lamellar keratoplasty (PKP), using human corneal donor tissue
  • PGP penetrating lamellar keratoplasty
  • This surgical technique is considered safe and effective, however one of the risks includes graft rejection as well as viral and bacterial infections transmitted through the donor corneal tissue
  • the overall number of transplant surgical procedures that can be performed is limited by the availability of donor corneas for transplantation
  • the present invention provides methods for improving corneal clarity ortreatmg corneal scars, corneal opacification, and optical aberrations including corneal haze by administering to the eye an agent selected from urea, a urea derivative, nonenzymatic protein urea, non-enzymatic proteins, nucleosides, nucleotides and their derivatives (e g , adenine, adenosine, cytosine, cytadine, guanine, guanitadine, guanidine, guanidinium chloride, guanidinium salts, thymidine, thymitadme, uradine, uracil cysteme), reduced thioctic acid, uric acid, calcium acetyl sahcylate, ammonium sulfate or other compounds capable of causing non enzymatic dissolution of the proteoglycans or any possible combination thereof, in an amount that is effective to accelerate the
  • Hassell et al analyzed corneal specimens obtained during surgery from patients with macular corneal dystrophy
  • Hassell et al found that cells from normal corneas synthesized both a chondroitin sulfate proteoglycan and a keratan sulfate proteoglycan similar to those present in monkey and bovine corneas
  • Cells in macular corneal dystrophy synthesized a normal chondroitin sulfate proteoglycan, but did not synthesize either keratan sulfate or a mature keratan sulfate proteoglycan Instead, the cells synthesized a glycoprotein with an unusually large oligosaccharide side chain
  • the transparency of the cornea may be altered in a manner more subtle than that seen in the corneal traumas described above
  • the appearance of optical, monochromatic aberrations may decrease the visual acuity (VA) of a subject's eye
  • VA visual acuity
  • the visual acuity of the human eye could be 20/10 or better, however, such good acuity is rarely obtained
  • Two optical conditions account for the sub-optimal level of visual acuity are diffraction due to pupil size and monochromatic aberrations
  • the limitations of visual acuity caused by diffraction decreases with increasing pupil diameter and may play an important role only for pupils smaller than 2mm
  • the optical errors of higher order (aberrations) of the human eye demonstrate an opposite behavior and may increase with larger pupil diameter
  • Refractive surgery for myopia and astigmatism such as radial keratotomy (RK), photorefractive keratectomy (PRK), and laser in situ keratomileusis (LASIK)
  • RK radial keratotomy
  • PRK photorefractive keratectomy
  • LASIK laser in situ keratomileusis
  • the present invention provides a new chemical method for the elimination of corneal aberrations and corneal collagen fiber disorganization resulting from accidental traumatic injury to the cornea or from refractive surgery for myopia, hyperopia and astigmatism, such as radial keratotomy (RK), photorefractive keratectomy (PRK), and laser in situ keratomileusis (LASIK), laser epithelial keratomileusis (LASEK) so as to improve visual acuity and quality of vision
  • RK radial keratotomy
  • PRK photorefractive keratectomy
  • LASIK laser in situ keratomileusis
  • LASEK laser epithelial keratomileusis
  • the present invention provides a new method for treating corneal ptengia by administering to the cornea an agent selected from urea, a urea derivative, nonenzymatic protein urea, non-enzymatic proteins, nucleosides, nucleotides and their derivatives (e g , adenine, adenosine, cytosine, cytadine, guanine, guanitadine, guanidine, guanidinium chloride, guanidinium salts, thymidine, thymitadme, uradine, uracil, cysteme), reduced thioctic acid, uric acid, calcium acetyl sahcylate, ammonium sulfate or other compounds capable of causing non enzymatic dissolution of the proteoglycans or any possible combination thereof, in an amount that is effective to inhibit expression of MMP-1 and MMP-3 by fibroblasts
  • an agent selected from
  • UV-B radiation appears to be an etiologic agent for ptengia and hmbal tumors
  • Ptengia begin growing from hmbal epithelium and not conjunctival epithelium
  • a segment of the mbal epithelium invades the cornea centnpetally followed by conjunctival epithelium
  • a distinct type of corneal cells develops at the leading edge of the ptengia tissue
  • Bowman's layer is dissolved under the leading edge of the ptengia
  • Vascularization occurs in the conjunctiva adjacent to the ptengia
  • Ptengia have a high recurrence rate
  • Ptengia are tumors of altered hmbal basal cells that secrete TGF- ⁇ and produce various types of MMP's similar to other invasive tumors
  • the tumor cell proteases degrade components of their basement membranes, which facilitate invasion
  • the pterigium cells invade over the Bowman's layer producing elevated MMP-1 , MMP-2 and MMP-9 which contribute to the complete dissolution of Bowman's layer
  • Local fibroblasts are activated by the TGF- ⁇ and bFGF cytokine pathways to help complete the dissolution of the Bowman's layer by MMP-1
  • the present invention provides a new method for treating corneal ptengia by administering to the cornea an agent selected from urea, a urea derivative, nonenzymatic protein urea, non-enzymatic proteins, nucleosides, nucleotides and their derivatives (e g , adenine, adenosine, cytosine, cytadine, guanine, guanitadine, guanidine, guanidinium chloride, guanidinium salts, thymidine, thymitadme, uradine, uracil, cysteme), reduced thioctic acid, uric acid, calcium acetyl sahcylate, ammonium sulfate or other compounds capable of causing non enzymatic dissolution of the proteoglycans or any possible combination thereof, in an amount that is effective to inhibit neovascularization of the cornea
  • an agent selected from urea,
  • the cornea may be invaded by leucocytes and fibrocytes, the nutritive supply and metabolic reserves may become inadequate with the result that new vessels sprout from the hmbal plexus and grow into the stroma, thus resulting in corneal vascularization
  • the nature of the stimulus to ingrowth of vessels has been associated with the loosening of the tissue associated with the injury and the resulting of corneal edema
  • the primary factor of corneal new vascularization is associated with the buildup and release of pharmacologically active angiogenic compounds like VEGF and FGF that are responsible for the formation of new vessels so as to supply the needs of the cornea
  • VEGF and FGF pharmacologically active angiogenic compounds like VEGF and FGF that are responsible for the formation of new vessels so as to supply the needs of the cornea
  • the presence of new blood vessels in the cornea makes the cornea full of vessels and interferes with the visual acuity of the patient
  • injuries in the posterior part of the eye and reduction of supply of oxygen to the retina and the optic nerve unleash
  • the present invention provides methods fortreatmg glaucoma by administering topically onto the eye or by injection into the eye (e g mtravitreal, intrastromal or sub- conjunctival injection) a therapeutically effective amount of an aqueous solution containing an agent selected from urea, a urea derivative, non-enzymatic protein urea, non-enzymatic proteins, nucleosides, nucleotides and their derivatives (e g , adenine, adenosine, cytosine, cytadine, guanine, guanitadine, guanidine, guanidinium chloride, guanidinium salts, thymidine, thymitadme, uradine, uracil, cysteme), reduced thioctic acid, uric acid, calcium acetyl sahcylate, ammonium sulfate or other compounds capable of causing
  • the structure of the optic nerve head may play a role in the pathogenesis of glaucoma
  • Two main theories exist for the mechanism of optic nerve damage in glaucoma the mechanical IOP related theory suggests that the pressure head acts directly on the lamina c ⁇ bosa The lamina c ⁇ bosa is not supported well superiorly and interiorly at the disk and it is here that the initial damage occurs to produce the characteristic arcuate defects Variations in the ganglion cell support at the disk may explain the variations between IOP susceptibilities of individuals with similar lOP's
  • Second is the vascular mechanism theory, which posits that changes within the microcirculation of the disk capillaries are responsible for glaucomatous changes whether this is primarily vascular or secondary to IOP has not been elucidated
  • the present invention provides urea containing solutions (e g , solutions which contain urea, a urea derivative (e g hydroxyurea) and /or mixtures thereof) that may be topically applied or are injected into the eye Additionally, some of the urea- containing topical or mjectable solutions of the present invention may further contain ant ⁇ metabohte(s) (e g mitomicyn C, methotrexate, 6-mercaptopurine, thioguanine, 5- fluorouracil, cytosine arabmoside and 5-azacyt ⁇ d ⁇ ne) Solutions of urea or hydroxyurea, which have been adjusted to a pH of approximately 4 0 to 8 0 are substantially non-toxic and well tolerated when administered topically, or by mtravitreal, intrastromal and conjunctival injection, one (1 ), two (2) or more times, in a volume of 15 to 200 microhters per application, at doses of 0 001 % to 4 0% and also
  • Citrate, phosphate or other buffers may alternatively be used in the above-listed formulations of Examples of 1-7 Also, sodium chloride, dextrose or other alternative bulking agents could be used in these formulations
  • Example 14 Urea USP/NF 001% -200% Polyethylene Glycol 010% -500% Sodium Chloride USP/NF 010% -090% Sterile Water for Injection USP QS 100% pH ofthe solution 40 - 80 (Adjust pH using 01 N HCI or 01 N NaOH)
  • Example 16 Hydroxyurea USP/NF 40% Sodium Chloride USP/NF 010% -090% Sterile Water for Injection USP QS 100% pH of the solution 40 - 80
  • Example 17 Hydroxyurea USP/NF 001% -150% Sodium Chloride USP/NF 010% -090% Sterile Water for Injection USP QS 100% pH ofthe solution 40 - 80
  • Antimetabohte solutions that are usable in accordance with the present invention
  • these antimetabohte soulitions may be combined with the aqueous solution of urea or other agent or the antimetabohte solution may be administered topically or injected separate from the aqueous solution of urea or other agent

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Abstract

Methods and preparations for treating disorders of the eye and/or causing dissolution of corneal proteoglycans and organized healing of corneal stroma, softening of the cornea for non-surgical refractive correction of eyesight, removing corneal haze and opacification, inhibiting fibroblasts and preventing corneal fibrosis and scar formation, treating pterigiums and treating corneal neovascularization as well as iris neovascularization. Preparations containing a) urea, b) urea derivatives (e.g., hydroxyurea, thiourea), c) antimetabolites, e) urea, urea derivatives, non-enzymatic proteins, nucleosides, nucleotides and their derivatives (e.g., adenine, adenosine, cytosine, cytadine, guanine, guanitadine, guanidinium, guanidinium chloride, guanidinium salts, thymidine, thymitadine, uradine, uracil, cysteine), reduced thioctic acid, uric acid, calcium acetyl salicylate, ammonium sulfate, isopropyl alcohol, ethanol, polyethylene glycol, polypropylene glycol or other compounds capable of causing nonenzymatic dissoultion of the corneal protoeglycans or f) any of the possible combinations thereof, are administered to the eye in therapeutically effective amounts.

Description

AGENTS FOR CORNEAL OR INTRASTROMAL
ADMINISTRATION TO TREAT OR PREVENT
DISORDERS OF THE EYE
CROSS-REFERENCE TO RELATED APPLICATIONS
This application claims priority to United States Provisional Application 60/363,979 filed on March 14, 2003, which is expressly incorporated herein by reference This application is also a continuation-in-part of copending United States Patent Application Serial No 10/215,680 entitled Agents for Intavitreal Administration To Treat or Prevent Disorders of the Eye filed on August 9, 2002, which is a continuation of United States Patent Application Serial No 09/517,798 filed on March 2 2000 and now issued as United States Patent No 6,462,071 B1
FIELD OF THE INVENTION
The present invention relates generally to pharmaceutical preparations and medical treatment methods, and more particularly agents (i e Urea, Urea derivatives, non-steroidal anti-inflammatory drugs and Anti-metabolite drugs) used alone or in combinations with each other (or with other agents) to treat or prevent certain disorders of the eye
BACKGROUND OF THE INVENTION Prior Ophthalmologic Uses of Urea
United States Patent Nos 5,629,344 (Charlton) and 5,470,881 (Charlton) describe certain therapeutic applications of urea preparations to the eye These prior patents specifically describe non-aqueous ointments and other non-aqueous preparations of urea for use in the eye, pointing out that aqueous solutions of urea were believed to be impractical for use in the eye For example, these prior patents state as follows "One of the reasons urea has not been used in treating eye disorders is that it will hydrolyze in aqueous vehicles thus producing ammonia as a byproduct Ammonia is toxic to the eye, and thus urea in an aqueous solution would be impractical for use as an ophthalmic medicament " Thus, prior to Applicant's invention, aqueous solutions of urea or urea derivatives were thought to be unstable and potentially toxic to the eye Anatomic and Physical Properties of the Cornea
The cornea is the first and most powerful refracting surface of the optical system of the eye Production of a sharp image at the retinal receptors requires that the cornea be transparent and of the appropriate refractive power The average corneal thickness of a normal cornea is 0 56 mm in persons under 25 years of age, this thickness slowly increases with age to become 0 57 in persons over 65 years of age The cornea is somewhat thicker in the periphery than the center The thickness of the cornea is greatest after the eyes have been closed for some time, as after sleeping, this thickness decreases slightly when the eyes are opened and exposed to the dehydrating effects of the air
The cornea is composed of six layers a) Epithelium b) Basement membrane c) Bowman's membrane d) Stroma e) Descemet's membrane f) Endothe um a) Epithelium The epithelium consists of 5-6 layers of cells The most superficial cells are flat overlapping squamous cells The middle layer consists of cells that become more columnar as the deeper layers are approached The innermost layer (basal) is made up of columnar cells packed closely together All the cells are held together by a cement substance Also, the cell surfaces form processes that are fitted into corresponding indentations of adjacent cells and connected in places by attachment bodies called desmoso es The basal cells are connected to the basement membrane by hemidesmosomes The epithelium represents 10% of the total wet weight of the cornea Water in the epithelium represents 70% of the wet weight
Although the epithelium consists of 5-6 layers of cells, the healthy epithelium is very strongly attached to each other by desmosomes as well as to the Basement membrane by hemidesmosomes b) Basement membrane: Between the columnar epithelial cells and Bowman's membrane is a basement membrane from 60 - 65 nm thick The basement membrane has been examined histochemically and found to be similar to other basement membranes c) Bowman's membrane: Bowman's layer is a sheet of transparent tissue about 12μm thick, without structure as seen by light microscopy Under electron microscopy it appears to be made up of uniform fibrils, probably of collagenous material, running parallel to the surface Bowman's layer possesses little resistance to any pathologic process, and is easily destroyed and never regenerates d) Stroma: The Stroma comprises about 90% of the whole cornea The Stroma is composed of layers of lamellae, each of which runs the full length of the cornea, although the bundles interlace with one another, they are nearly parallel to the surface The cell bodies, called keratocytes, are flattened, so they too lie parallel to the surface, and their cell processes interlace with one another This arrangement of the fibers gives optical uniformity to the cornea The Stroma comprises about 90% of the whole cornea The Stroma is composed of differentiated connective tissue containing 75% to 80% water on a wet weight basis The remaining solids 20% to 25% is collagen, other proteins, and glycosaminoglycans or mucopolysacchaπdes constitute the major part The collagen fibrils are neatly organized and present the typical 64 to 66 nm periodicity of collagen fibrils separated from each other by the ground substance The size, regularity, and precise spacing of the fibπllar structures are the physical characteristics essential for corneal transparency
The glucosammoglycans (GAG, mucopolysacchaπdes) represent 4% to 4 5% of the dry weight of the cornea GAG are localized in the interfibπllar or interstitial space, probably attached to the collagen fibrils or to soluble proteins of the cornea The GAG plays a role in corneal hydration through interaction with electrolytes and water Three major GAG fractions are found in the corneal Stroma keratin sulfate (50%), chondroitin (25%), and chondroitin sulfate A (25%) GAG s have been implicated in the maintenance of the corneal hydration level and transparency e) Descemet's Membrane: Is made of type IV collagen, unlike the corneal Stroma, there are no significant amounts of sulfated GAG in the Descemet's membrane The collagen in this membrane is insoluble except in strong alkali or acid and is more resistant to collagenase than corneal stroma collagen Jakus2 has observed with the electron microscope that this membrane has collagen like structure of great regularity Descemet's membrane is highly elastic and represents a barrier to perforation in deep corneal ulcers f) Endothelium: The endothehum is a single layer of cells lining
Descemet's membrane Its inner surface is bathed by the aqueous humor In humans the endothelium cell layer has limited, if any, reproductive capacity Aging causes cell loss, and the remaining cells enlarge and spread so that Descemet's membrane remains completely covered therefore endothelial cell density, expressed as cells per unit area, decreases with age Similarly, cell loss from trauma, inflammation, or surgery is compensated for by increased cell size and decreased cell density Corneal metabolism embraces a series of chemical processes by which energy is obtained and utilized for the normal functions of the cornea In the cornea, energy is needed for maintenance of its transparency and dehydration Energy in the form of ATP is generated by the breakdown of glucose into lactic acid and into carbon dioxide and water (i e , Krebs Cycle) The cornea obtains glucose mainly from the aqueous humor The tears and hmbal capillaries appear to contribute minimal amounts of glucose and Oxygen for corneal metabolism
Most of the oxygen consumed by the cornea is taken in by the epithelium and the endothe um The oxygen consumption of the epithelium and endothehum can be approximately 26 times that of the stroma The corneal endothelium gets most of its required oxygen from the aqueous humor, while the corneal epithelium gets much of its oxygen from either the capillaries at the hmbus or from the oxygen dissolved in the pre-corneal film Methods for the Refractive Correction of the Eye:
Radial keratotomy (RK) is a surgical procedure to improve myopia by changing the corneal curvature This is achieved by making several deep incisions in the cornea in a radial pattern The eye surgeon makes 4, 8, or 16 incisions so as to flatten the curvature of the central cornea, thus correcting the patient's vision The main drawbacks of RK include, a) It can only be used to correct low levels of myopia b) This surgical procedure cannot correct hyperopia c) RK procedure seriously weakens the cornea and creates corneal scars d) The corneal curvature changes are temporary and frequently continue to change with time
Photorefractive keratectomy (PRK) is a surgical procedure that uses the excimer laser, which is controlled by a computer With the PRK procedure, the excimer laser ablates and sculpts the corneal surface to the desired shape to correct the patient's vision There are a combination of lasers with a combination of computer controls that can reliably treat myopia, hyperopia, and astigmatism Since PRK is a surgical procedure, it can result in complications Infection is the most serious complication resulting from the ablation of a large area of the corneal epithelium In addition delayed corneal healing because of the absence of the corneal epithelium corneal haze, corneal scarring, over correction or under-correction and development of astigmatism are other complications of PRK These complications must be treated with medications or further surgery
Laser in-situ keratomileusis11 (LASIK) is a surgical procedure that is a variation on PRK involving an excimer laser and a precise cutting tool called a microkeratome The microkeratome is used to make a 150-175 micron circular flap of the cornea The circular flap is flipped back, as if on a hinge, to expose the stromal layer of the cornea With the flap folded back, the refractive eye surgeon now ablates the stroma and makes the refractive correction using the excimer laser The circular corneal flap is repositioned on the ablated cornea to complete the procedure With a precision laser treatment and normal reattachment and healing of the corneal flap the refractive results of good vision correction are very rapid There is, however, a significant list of potential complications and risks associated with LASIK procedure, failure of the microkeratome to leave a hinge on the corneal flap with the first incision, loss of the corneal flap after the operation, slipping of the flap and healing off center, first incision is too deep or too shallow, corneal epithelium ingrowths into the stroma, infection of the cornea, corneal ectasia, loss of visual acuity from scarring and optical distortion of the collagen structure of the stroma
Laser epithelial keratomileusis (LASEK) is a surgical procedure that is a variation on PRK involving an excimer laser that combines the advantages and eliminates the disadvantages of PRK and LASIK A 7 0 mm circular area of the epithelium is marked with a Hoffer trephine centered over the pupil The corneal epithelium is removed by using a blunt spatula, or is exposed to 20% isopropyl alcohol solution which allows the corneal epithelium to be peeled off Using the excimer laser the surgeon ablates and sculpts the corneal surface to the desired shape to correct the patient's vision At the end of the procedure the corneal epithelial flap created by the alcoholic solution is placed back onto the ablated cornea, a drop of antibiotic, a drop of non-steroidal anti-inflammatory agent and a therapeutic contact lens is applied to the corrected eye The epithelial defect created by the scrapping of the corneal epithelium or by peeling of the epithelium after the application of alcoholic solution is completely closed within a few days With a precision laser treatment and normal healing of the corneal epithelium, the refractive results of good vision correction are very rapid There are, however, a few potential complications and risks associated with LASEK procedure, infection of the cornea because of the epithelial defect as a result of epithelial scrapping, use of alcoholic solution causes extensive damage to the peeled corneal epithelium minimizing the benefits of the reapphed corneal epithelium Ther okeratoplasty is another corneal reshaping method In this procedure heat at 55°C to 58°C is applied to the collagen fibers of the cornea to induce shrinkage without the destruction of the tissue The shrinkage of the collagen fibers result in the change of the mechanical properties and flattening of the cornea, thus achieving refractive correction U S Patent No 4,881 ,543 describes the use of microwave electromagnetic energy to shrink the collagen of the cornea U S Patent No 5,779,696 describes the use of light energy to reshape the cornea All of these systems of Thermokeratoplasty have a shortcoming that is the treated corneas are unstable after the treatment
Orthokeratology is a non-surgical procedure designed to correct refractive errors by reshaping the cornea to the corneal curvature required to achieve emmetropia This is accomplished by applying a series of hard contact lenses that change the corneal curvature until the desired curvature is achieved However once the desired curvature has been produced, retainer hard contact lenses must be worn to stabilize the results otherwise regression will occur
Enzyme Orthokeratology is related to traditional Orthokeratology in that it is defined primarily as a contact lens procedure of correcting refractive errors of the eye by reshaping the cornea to the curvature required for emmetropia The system is enhanced by enzymatically softening the cornea, and reshaping is obtained in a shorter period of time, and retainer lenses may not be required for good visual acuity after removal of the contact lens from the eye and regression will not be a problem
Chemical Orthokeratology is related to traditional Orthokeratology in that it is defined primarily as a contact lens procedure of correcting refractive errors of the eye by reshaping the cornea to the curvature required for emmetropia The system is enhanced by applying topically or by intra-stromal injection a chemical that is not an enzyme and softening the cornea, and reshaping is obtained in a shorter period of time, and retainer lenses may not be required for good visual acuity after removal of the contact lens from the eye and regression will not be a problem SUMMARY OF THE INVENTION The present invention provides methods for treating or preventing disorders of the eye of a human or veterinary patient by administering topically onto the eye or by injection into the eye (e g intravitreal, intrastromal or sub-conjunctival injection) a therapeutically effective amount of an aqueous solution containing an agent selected from urea, a urea derivative, non-enzymatic protein urea, non-enzymatic proteins, nucleosides, nucleotides and their derivatives (e g , adenine, adenosine, cytosine, cytadine, guanine, guanitadine, guanidine, guanidinium chloride, guanidinium salts, thymidine, thymitadme, uradine, ura l, cysteme), reduced thioctic acid, uric acid, calcium acetyl sa cylate, ammonium sulfate or other compounds capable of causing non enzymatic dissolution of the proteoglycans or any possible combination thereof Included among the therapeutic purposes for which this method may be used are removal of the corneal epithelium, dissolution of the corneal proteoglycans, interface closure and organized healing of corneal stroma in refractive LASIK correction, dissolution of proteins and ammo acids so as to compress the collagen fibrils for better visual acuity and better quality of vision, softening of the cornea prior to or during application of a contact lens or cornea-reshaping template for the non-surgical refractive correction of myopia, presbyopia, hyperopia, astigmatism and keratoconus, dissolution of newly synthesized proteoglycans thereby lessening or eliminating corneal haze and/or corneal opacification, dissolution of proteoglycans in the anterior chamber thereby increasing outflow of fluid which may lower of intra-ocular pressure in some glaucoma patients, causing a solvent action on fibroblasts, inhibiting fibroblasts, inhibiting or preventing corneal fibrosis and scar formation, inhibiting the proliferation of fibroblasts in ocular tissue, inhibiting VEGF activity in the cornea and the iris via an anti-angiogenic effect, thus eliminating both the progression and the regression of corneal new vessels and iris new vessels By one or more of these therapeutic effects and/or other mechanisms of action yet to be elucidated, the method of the present invention may be usable to treat various disorders of the eye As used in this patent application, the term "treat" shall not be limited only to treatment of existing diseases or disorders but also shall mean preventing, deterring, stopping, curing, or slowing the progression of such disorders The disorders of the eye that may be treated by the method of the present invention include but are not limited to refractive disorders, impaired visual acuity or diminished quality of vision, myopia, presbyopia, hyperopia, astigmatism, keratoconus, corneal fibrosis, scar formation, corneal opacities, pterigiums, corneal neovascularization, ins neovascularization, glaucoma
Further in accordance with the invention, the agent may be administered in combination with an antimetabolite compound such as, mitomicyn, methotrexate, thiourea, hydroxyurea, 6-mercaptopurιne, thioguanine, 5-fluorouracιl, cytosine arabinoside and 5-azacytιdιne
Still further in accordance with the invention, the agent may be administered in combination with an antmeoplastic agent such as Actinomycin D, daunorubicin, doxorubicin, idarubicin, bleomycins, or phcamycin may also be used in combination with these anti-metabolites
Still further aspects, objects and advantages of the invention will be apparent to those of skill in the art who read and understand the following detailed description of the invention and the specific examples set forth therein
DETAILED DESCRIPTION
The following detailed description and the examples referred to therein are provided for the purpose of describing certain embodiments or examples of the invention only and shall not be construed as limiting the scope of the invention in any way Removal of the Corneal Epithelium
One example of an application of the method of the present invention is for the removal of corneal epithelium As explained above, the corneal epithelial cells are held together by a cement substance In addition the surfaces of the cells form processes that are fitted into the corresponding indentations of the adjacent cells and connected by attachment bodies called desmosomes In addition the basal cells of the epithelium are connected to the basement membrane by hemidesmosomes When the corneal epithelium is damaged by chemical or physical means, swelling of the stroma follows Abrasion of the cornea or any condition leading to the loss of epithelium is likely to produce localized areas of corneal swelling and cloudiness and allows microbial access and bacterial infections Fortunately, the corneal epithelium regenerates rapidly, and the excessive hydration and wound closure in the absence of bacterial infections is slight and transient
Effecting mechanical or chemical de-epithehahzation (debπdement) while keeping the epithelium in tact an without damage is not an easy task There are several methods that are utilized presently, but all these methods and materials cause severe damage to the corneal epithelium Mechanical de-epithehahzation is typically performed under topical anesthesia with a local anesthetic with a blunt spatula after the epithelium is marked with a 7 0 mm Hoffer trephine centered overthe pupil The resulting corneal wound usually takes several days to re-epithelialize During this time any exposed corneal incision or wound is susceptible to bacterial contamination and infection Chemical de-epithehahzation using alcohol is also typically performed under topical anesthesia with a local anesthetic The epithelium is marked, and by a gentle depression on the trephine a circular cut is made with a 7 0 mm Hoffer trephine centered over the pupil While the trephine is in place 5 - 10 drops of 20% Isopropyl Alcohol are dispensed into the trephine and kept in contact with the epithelium for several minutes The alcoholic solution is removed with a dry sponge, and the trephine is removed from the cornea Using a blunt spatula the epithelium is removed intact in one piece This procedure is a simple way to de-epithehahze the cornea, however 50% - 70% of the epithelial cells are damaged because of the exposure to the alcoholic solution Furthermore the 20% alcoholic solution is very irritating and inflammatory to the eye After the surgical procedure, the resulting corneal wound is covered with the single piece of the alcohol removed epithelium The resulting wound is temporarily covered with corneal epithelium which will take several days to re-epithehahze During this time of wound healing the cornea is less susceptible to bacterial contamination and infection In accordance with the present invention, there is provided a new method for chemical removal of the corneal epithelium using an agent selected from urea, a urea derivative, non-enzymatic protein urea, non-enzymatic proteins, nucleosides, nucleotides and their derivatives (e g , adenine, adenosine, cytosine, cytadine, guanine, guanitadine, guanidine, guanidinium chloride, guanidinium salts, thymidine, thymitadme, uradine, uracil, cysteme), reduced thioctic acid, uric acid, calcium acetyl sahcylate, ammonium sulfate or other compounds capable of causing non enzymatic dissolution of the proteoglycans or any possible combination thereof This method may be performed under topical anesthesia with a local anesthetic First, the epithelium is marked, and by a gentle depression on the trephine a circular cut is made with a 7 0 mm Hoffer trephine centered over the pupil While the trephine is in place 5 - 10 drops of the agent (e g , 0 01 % - 20% of aqueous urea solution) is dispensed into the trephine and kept in contact with the epithelium for several minutes The agent (e g , aqueous urea solution) is removed with a dry sponge, and the trephine is removed from the cornea Using a blunt spatula the epithelium is removed intact in one piece This procedure is a simple way to de-epithehahze the cornea resulting in no damage to the epithelial cells After the surgical procedure, the resulting corneal wound is covered with the single piece of the urea removed epithelium The resulting wound is temporarily covered with corneal epithelium which will re- epithehahze in 1-2 days During this time of wound healing the cornea is less susceptible to bacterial contamination and infection This chemical de-epithehahzation of the cornea using an agent of the present invention (e g , urea solution) may be useful as an adjunct to ophthalmic surgery for the treatment of herpetic epithelial keratitis, as well as for refractive correction of vision using the Laser epithelial keratomileusis (LESEK)
Enhanced Corneal Interface closure and Organized Healing of the corneal stroma in Refractive LASIK Correction. The present invention also provides methods for enhancing healing of the cornea after LASEK surgery In this method, an agent selected from urea, a urea derivative, non-enzymatic protein urea, non-enzymatic proteins, nucleosides, nucleotides and their derivatives (e g , adenine, adenosine, cytosine, cytadine, guanine, guanitadine, guanidine, guanidinium chloride, guanidinium salts, thymidine, thymitadme uradine, uracil, cysteme), reduced thioctic acid, uric acid, calcium acetyl sahcylate, ammonium sulfate or other compounds capable of causing non enzymatic dissolution of the proteoglycans or any possible combination thereof, is applied topically to the cornea following a LASIK procedure For example, a few drops of the agent (e g , 0 01 % - 20 0% aqueous urea solution) may be placed onto the surface of the excimer laser ablated stroma before the cut flap of the corneal epithelium is repositioned on the laser ablated cornea The urea solution placed at the interface of the corneal epithelium and stroma, will result in the localized solubilization of the stromal proteoglycans and will compress the collagen fibril packing for better visual performance, but normal transparency
Successful completion of Laser m-situ keratomileusis (LASIK) refractive correction results in the precision cutting of the cornea, excimer laser ablation of the stroma and the repositioning of the circular flap on the ablated cornea Normal reattachment and healing of the corneal flap are very important parameters for good vision correction and rapid healing The superficial placement of the microkeratome cut circular flap of the cornea onto the excimer laser ablated stroma results in an interface gap in the stroma between the upper and lower parts of the stroma This interface gap interferes with optimum vision correction, in addition the interface gap never completely comes together as a single stoma indicating the lack of complete wound healing of the cornea
In the present invention of enhanced corneal interface closure and organized healing of the LASIK refractive corrected cornea, a few drops of an agent of the present invention (e g , 0 01 % - 20 0% aqueous urea solution) is placed onto the surface of the excimer laser ablated stroma before the microkeratome cut flap of the cornea is repositioned on the laser ablated cornea The urea solution placed at the interface of the two corneal flaps will result in the localized solubilization the stromal proteoglycans and eliminate the interface gap, thus producing optimum vision correction In addition the localized solubilization of the proteoglycans of the stroma will result in the compression of the collagen fibril packing for better visual performance, but normal transparency
Softening of the Corneal Stroma by topical or intrastromal application, for the non-surgical Refractive correction of Myopia, Presbyopia, Hyperopia, Astigmatism and Keratoconus The present invention also provides methods for softening the cornea by administering to the cornea an agent selected from urea, a urea derivative, nonenzymatic protein urea, non-enzymatic proteins, nucleosides, nucleotides and their derivatives (e g , adenine, adenosine, cytosine, cytadine, guanine, guanitadine, guanidine, guanidinium chloride, guanidinium salts, thymidine, thymitadme, uradine, uracil cysteme) reduced thioctic acid, uric acid, calcium acetyl sahcylate, ammonium sulfate or other compounds capable of causing non enzymatic dissolution of the proteoglycans or any possible combination thereof , in an amount thta is effective to cause temporary softening of the cornea so that it can be reshaped from a first configuration to a desired second configuration of emmetropia The softening of the cornea could take place while the patient is wearing rigid contact lenses having a concave shape of the desired second configuration rendering the eye emmetropic The cornea is thereafter permitted to shape to the desired second configuration under the influence of the lens Since the corneal softening is a result of localized solubilization of the proteoglycans and not the chemical breakdown of the of the proteoglycan molecules, it is possible that the corneal softening effect of the agent will dissipate much faster in the presence or absence of the molding rigid lens
The shape of the cornea is based on the collagen fibrils of the stroma which are held in place at a much specified distance from each other in parallel along with the mucopolysacchandes cement layers between these collagen fibrils The Urea and Urea derivatives have the ability of solubi zing the mucopolysacchandes as well as various proteins The stroma is therefore softened and becomes more pliable and easy to mold to a more desirable shape
In the preferred embodiment, the cornea softening agent comprises urea or a urea derivative together with pharmaceutically acceptable carriers and additives The preparation may be supplied in a liquid or lyophilized form The cornea softening agent in accordance with the present invention is administered to the cornea in a number of ways Typically, the agent is administered either directly in the form of eye drops, or by the use of a corneal softening agent delivery vehicle, which may include special drug delivery systems including hposomes, sustained release gels and implantable solid dosage forms as well as contact lens and biodegradable corneal collagen shield Non-Surgical Treatment and Elimination of Corneal Haze and Corneal Opacification
A reduction of visual acuity and blindness may result from a lack of corneal clarity caused by corneal traumas, corneal scars, or any other cause of corneal opacification Patients who have a reduction of visual acuity as a result of corneal opacities are estimated to be three million The current treatment for corneal opacity is corneal transplantation using a surgical procedure called penetrating lamellar keratoplasty (PKP), using human corneal donor tissue This surgical technique is considered safe and effective, however one of the risks includes graft rejection as well as viral and bacterial infections transmitted through the donor corneal tissue The overall number of transplant surgical procedures that can be performed is limited by the availability of donor corneas for transplantation
The present invention provides methods for improving corneal clarity ortreatmg corneal scars, corneal opacification, and optical aberrations including corneal haze by administering to the eye an agent selected from urea, a urea derivative, nonenzymatic protein urea, non-enzymatic proteins, nucleosides, nucleotides and their derivatives (e g , adenine, adenosine, cytosine, cytadine, guanine, guanitadine, guanidine, guanidinium chloride, guanidinium salts, thymidine, thymitadme, uradine, uracil cysteme), reduced thioctic acid, uric acid, calcium acetyl sahcylate, ammonium sulfate or other compounds capable of causing non enzymatic dissolution of the proteoglycans or any possible combination thereof, in an amount that is effective to accelerate the solubilization of corneal proteoglycans, mucopolysacchandes and various other proteins and lead to the reorganization of corneal collagen The resulting reorganization will clear corneal scars, corneal opacities and corneal haze For example, the agent (e g , an aqueous solution of urea or a urea derivative) may be administered topically or by injection in an amount that reduces corneal collagen disorganization by chemical modification or dissolution of corneal stromal glycoprotem's and proteoglycans The role of corneal glycoprotem's and proteoglycans in the establishment and maintenance of comeal transparency is not well understood Stromal proteoglycans have been hypothesized to play a role in the regulation of collagen fiber spacing Although the precise role of proteoglycans is still unclear, they are thought to influence the hydration thickness and clarity of the cornea The functional significance of hyaluronan in the cornea, except during development and in some corneal abnormalities is still unknown In some opaque human corneal scars, the scars have been found to contain collagen fibrils with abnormally large diameter and irregular interfibrillar spacing However, during wound healing of rabbit corneas, the early opaque scars contain collagen fibrils of generally normal diameter that are irregularly spaced within the tissue The collagen fibril diameter does not markedly change after a year of healing, but the spacing between the fibrils returns to normal and there is a concomitant decrease in the opacity of the scar
In a 1983 paper authored by Hassell et al , showed that opaque scars that contained the large interfibrillar spaces also contained unusually large chondroitin sulfate proteoglycans with glycosaminoglycan side chains of normal size These opaque scars also lacked the keratan sulfate proteoglycan but did contain hyaluronic acid The biochemical analysis of proteoglycans in rabbit corneal scars in corneal wounds compared to normal cornea adjacent to the scar demonstrates that the areas synthesize proteoglycans measurably different from one another
Hassell et al analyzed corneal specimens obtained during surgery from patients with macular corneal dystrophy Hassell et al found that cells from normal corneas synthesized both a chondroitin sulfate proteoglycan and a keratan sulfate proteoglycan similar to those present in monkey and bovine corneas Cells in macular corneal dystrophy synthesized a normal chondroitin sulfate proteoglycan, but did not synthesize either keratan sulfate or a mature keratan sulfate proteoglycan Instead, the cells synthesized a glycoprotein with an unusually large oligosaccharide side chain
The transparency of the cornea may be altered in a manner more subtle than that seen in the corneal traumas described above In certain situations the appearance of optical, monochromatic aberrations may decrease the visual acuity (VA) of a subject's eye On the basis of the mosaic structures of the retina, the visual acuity of the human eye could be 20/10 or better, however, such good acuity is rarely obtained Two optical conditions account for the sub-optimal level of visual acuity are diffraction due to pupil size and monochromatic aberrations The limitations of visual acuity caused by diffraction decreases with increasing pupil diameter and may play an important role only for pupils smaller than 2mm The optical errors of higher order (aberrations) of the human eye, however, demonstrate an opposite behavior and may increase with larger pupil diameter
The shape of the human cornea and lens is naturally designed in a way that these aberrations are minimized To our knowledge, the monochromatic aberrations of the human eye so far have not been studied systematically in large series of individuals Therefore, average values for a standard population are not available However, loss of visual acuity through the introduction of optical aberrations may become clinically relevant with the advent of refractive corrective surgery
Refractive surgery for myopia and astigmatism, such as radial keratotomy (RK), photorefractive keratectomy (PRK), and laser in situ keratomileusis (LASIK), induce a non-physiological corneal shape with a flat central area and increasing power towards the periphery This shape induces an increase in optical aberrations and may lead to visual losses that are detected under low lighting conditions, and by low contrast visual acuity testing These side effects of corneal refractive surgery have the potential for public health problems of a yet unknown discussion
Comparison of corneal wavefront aberrations after PRK and LASIK has been compared in a prospective randomized study of 22 patients with bilateral myopia who received PRK on one eye and LASIK in the other eye Before surgery, simulated papillary dilation from 3mm to 7mm caused a five to six fold increase in the total aberrations After surgery, the same dilation resulted in a 25 to 32 fold increase in the total aberrations in the PRK group and a 28 to 46 fold increase in total aberrations in the LASIK Both photorefractive keratectomy and laser in situ keratomileusis significantly increased the total wavefront aberrations and the values did not return to the preoperative level throughout the 12-month follow-up period
Corneal wound healing study in rabbits following LASIK to evaluate the corneal wound healing process was followed for 1 , 2 and 9 months past LASIK surgery Periodic histopathological evaluation of the rabbit corneas showed disorganized collagen fibers along the interface of the corneal flap even 9 months after the LASIK surgery These results show that the corneal aberrations and the wound healing process induced by the LASIK surgery continued at 9 months after LASIK The methods and compositions of the invention disclosed herein provide the means with which to overcome the optical aberration side-effect of modern refractive surgical techniques Without being restricted to any particular mechanism of action, it has been theorized that the various corneal aberrations resulting from RK, PRK, LASIK, LASEK and other surgical procedures result from corneal collagen disorganization that occurs during the healing process For example, following the LASIK procedure, after the flap is positioned to cover the site of the surgical procedure, corneal collagen will be formed to seal the incision As this collagen is formed it is thought to be arraigned in a conformation that is, to one degree or another, less organized than the collagen located in areas of the cornea not affected by the surgery Reorganization of this material would lead to a reduction in optimal aberrations resulting from such surgeries
Accordingly, the present invention provides a new chemical method for the elimination of corneal aberrations and corneal collagen fiber disorganization resulting from accidental traumatic injury to the cornea or from refractive surgery for myopia, hyperopia and astigmatism, such as radial keratotomy (RK), photorefractive keratectomy (PRK), and laser in situ keratomileusis (LASIK), laser epithelial keratomileusis (LASEK) so as to improve visual acuity and quality of vision
Non-Surgical Treatment of Pterigium
The present invention provides a new method for treating corneal ptengia by administering to the cornea an agent selected from urea, a urea derivative, nonenzymatic protein urea, non-enzymatic proteins, nucleosides, nucleotides and their derivatives (e g , adenine, adenosine, cytosine, cytadine, guanine, guanitadine, guanidine, guanidinium chloride, guanidinium salts, thymidine, thymitadme, uradine, uracil, cysteme), reduced thioctic acid, uric acid, calcium acetyl sahcylate, ammonium sulfate or other compounds capable of causing non enzymatic dissolution of the proteoglycans or any possible combination thereof, in an amount that is effective to inhibit expression of MMP-1 and MMP-3 by fibroblasts For example, one may administer to a corneal pterigium a therapeutically effective amount of an aqueous solution of urea or a urea derivative so as to stop or slow the expression of MMP-1 and MMP-3 by fibroblasts within the cornea The urea and urea derivatives have the ability of deactivating the enzymatic activity of the expressed MMP's It is also recognized and documented that urea and urea derivatives by their ability to solubi ze proteins will change the secondary and tertiary structure of proteins thus inactivating these proteins The resulting solubilization of proteins by Urea and Urea derivatives will clear corneal ptengia, stop the dissolution of the Bowman's layer and stop and produce regression of corneal Neovascularization of the cornea
Several important clinical and pathological characteristics of primary and recurrent ptengia have been identified These include the following a) UV-B radiation appears to be an etiologic agent for ptengia and hmbal tumors b) Ptengia begin growing from hmbal epithelium and not conjunctival epithelium c) A segment of the mbal epithelium invades the cornea centnpetally followed by conjunctival epithelium d) A distinct type of corneal cells develops at the leading edge of the ptengia tissue e) Bowman's layer is dissolved under the leading edge of the ptengia f) Vascularization occurs in the conjunctiva adjacent to the ptengia g) Ptengia have a high recurrence rate
As in most normal, resting tissues, conjunctival- mbal-corneal epithelial tissue expresses very small amounts of MMP's that are nearly undetectable by immunohistochemistry techniques However, it has been demonstrated lately that the altered hmbal basal epithelial cells of ptengia express 6 MMP's of various types similar to other invasive tumors It is speculated that these MMP's are likely promoters of the corneal invasion of this tumor and contribute to the dissolution of Bowman's layer Elevated expression of both MMP-2 and MMP-9 are known to dissolve basement membrane components, such as hemidesmosomes, leading to migration and invasion of tumor cells In addition four different groups of fibroblasts were identified in ptengia These fibroblasts expressed mainly MMP-1 and some MMP-3
Ptengia are tumors of altered hmbal basal cells that secrete TGF-β and produce various types of MMP's similar to other invasive tumors The tumor cell proteases degrade components of their basement membranes, which facilitate invasion The pterigium cells invade over the Bowman's layer producing elevated MMP-1 , MMP-2 and MMP-9 which contribute to the complete dissolution of Bowman's layer Local fibroblasts are activated by the TGF-β and bFGF cytokine pathways to help complete the dissolution of the Bowman's layer by MMP-1
Treatment of Corneal and Iris Neovascularization The present invention provides a new method for treating corneal ptengia by administering to the cornea an agent selected from urea, a urea derivative, nonenzymatic protein urea, non-enzymatic proteins, nucleosides, nucleotides and their derivatives (e g , adenine, adenosine, cytosine, cytadine, guanine, guanitadine, guanidine, guanidinium chloride, guanidinium salts, thymidine, thymitadme, uradine, uracil, cysteme), reduced thioctic acid, uric acid, calcium acetyl sahcylate, ammonium sulfate or other compounds capable of causing non enzymatic dissolution of the proteoglycans or any possible combination thereof, in an amount that is effective to inhibit neovascularization of the cornea and/or ins
Under conditions of metabolic and injury-related stress, the cornea may be invaded by leucocytes and fibrocytes, the nutritive supply and metabolic reserves may become inadequate with the result that new vessels sprout from the hmbal plexus and grow into the stroma, thus resulting in corneal vascularization The nature of the stimulus to ingrowth of vessels has been associated with the loosening of the tissue associated with the injury and the resulting of corneal edema However, the primary factor of corneal new vascularization is associated with the buildup and release of pharmacologically active angiogenic compounds like VEGF and FGF that are responsible for the formation of new vessels so as to supply the needs of the cornea The presence of new blood vessels in the cornea makes the cornea full of vessels and interferes with the visual acuity of the patient In a similar fashion, injuries in the posterior part of the eye and reduction of supply of oxygen to the retina and the optic nerve, unleashes the buildup of VEGF angiogenic factors in the vitreous The result is the new vessel formation in the ins, causing bleeding and blindness
Treatment of Glaucoma The present invention provides methods fortreatmg glaucoma by administering topically onto the eye or by injection into the eye (e g mtravitreal, intrastromal or sub- conjunctival injection) a therapeutically effective amount of an aqueous solution containing an agent selected from urea, a urea derivative, non-enzymatic protein urea, non-enzymatic proteins, nucleosides, nucleotides and their derivatives (e g , adenine, adenosine, cytosine, cytadine, guanine, guanitadine, guanidine, guanidinium chloride, guanidinium salts, thymidine, thymitadme, uradine, uracil, cysteme), reduced thioctic acid, uric acid, calcium acetyl sahcylate, ammonium sulfate or other compounds capable of causing non enzymatic dissolution ofthe proteoglycans or any possible combination thereof
As well as being an important marker of the presence and advancement of glaucoma, the structure of the optic nerve head may play a role in the pathogenesis of glaucoma Two main theories exist for the mechanism of optic nerve damage in glaucoma First, the mechanical IOP related theory suggests that the pressure head acts directly on the lamina cπbosa The lamina cπbosa is not supported well superiorly and interiorly at the disk and it is here that the initial damage occurs to produce the characteristic arcuate defects Variations in the ganglion cell support at the disk may explain the variations between IOP susceptibilities of individuals with similar lOP's Second is the vascular mechanism theory, which posits that changes within the microcirculation of the disk capillaries are responsible for glaucomatous changes whether this is primarily vascular or secondary to IOP has not been elucidated
The present invention provides urea containing solutions (e g , solutions which contain urea, a urea derivative (e g hydroxyurea) and /or mixtures thereof) that may be topically applied or are injected into the eye Additionally, some of the urea- containing topical or mjectable solutions of the present invention may further contain antιmetabohte(s) (e g mitomicyn C, methotrexate, 6-mercaptopurine, thioguanine, 5- fluorouracil, cytosine arabmoside and 5-azacytιdιne) Solutions of urea or hydroxyurea, which have been adjusted to a pH of approximately 4 0 to 8 0 are substantially non-toxic and well tolerated when administered topically, or by mtravitreal, intrastromal and conjunctival injection, one (1 ), two (2) or more times, in a volume of 15 to 200 microhters per application, at doses of 0 001 % to 4 0% and also doses of 0 001 % to 20 0% of urea
Examples of Stable Aqueous Urea Formulations
The following are examples of urea containing solutions that are usable in accordance with this invention Example 1
Urea USP/NF 0001 - 40%
Sodium Chloride USP/NF 01%- 09%
Sterile Water for Injection USP QS 100% pH ofthe solution 40 - 80
(Adjust pH using 01N HCI or 01N NaOH)
Example 2 Urea USP/NF 0001 -40% Citric Acid USP/NF 000007% - 002% Sodium Chloride USP/NF 01%- 09% Sterile Water for Injection USP QS 100% pH ofthe solution 40 - 80
(Adjust pH using 01 N HCI or 01 N NaOH)
Example 3
Urea USP/NF 0001 - 40%
Citric Acid USP/NF 000007% - 002%
Sterile Water for injection USP QS 100% pH ofthe solution 40 - 80
(Adjust pH using 01N HCI or 01N NaOH)
Example 4
Urea USP/NF 001 - 200%
Sodium Chloride USP/NF 01%- 09%
Sterile Water for Injection USP QS 100% pH ofthe solution 40 - 80
(Adjust pH using 01 N HCI or 01 N NaOH)
Example 5
Urea USP/NF 001 - 200%
Citric Acid USP/NF 000007% - 002%
Sodium Chloride USP/NF 01%- 09%
Sterile Water for Injection USP QS 100% pH of the solution 40 - 80
(Adjust pH using 01 N HCI or 01 N NaOH)
Example 6 Urea USP/NF 40%
Potassium Phosphate Dibasic USP/NF 50 milhmolar Sterile Water for Injection USP QS 100% pH ofthe solution 40 - 80 (Adjust pH using 0 1 N HCI or 0 1 N NaOH)
Example 7
Urea USP/NF 4 0%
Potassium Phosphate Dibasic USP/NF 50 0 milhmolar
Sterile Water for Injection USP Q S 100% pH of the solution 4 0 - 8 0
(Adjust pH using 0 1 N HCI or 0 1 N NaOH)
Example 8 (Lyophihzed Powder)
Urea USP/NF 0 01 % - 20 0%
Sorbitol USP/NF 0 10% - 0 50%
Citric Acid USP/NF 0 00007% - 0 02%
Sterile Water for Injection USP Q S 100% pH of the solution 4 0 - 8 0
(Adjust pH using 0 1 N HCI or 0 1 N NaOH)
Example 9 Urea USP/NF 4 0% Sorbitol USP/NF 0 10% Sterile Water for Injection USP Q S 100% pH of the solution 4 0 - 8 0
(Adjust pH using 0 1 N HCI or 0 1 N NaOH)
Citrate, phosphate or other buffers may alternatively be used in the above-listed formulations of Examples of 1-7 Also, sodium chloride, dextrose or other alternative bulking agents could be used in these formulations
Examples of Aqueous Urea Solutions Containing Alcohols and Block Polymeric Polyols
Example 10
Urea USP/NF 0 01 % - 20 0%
Isopropyl Alcohol (90%) 0 5% - 20%
Sodium Chloride USP/NF 0 1 % - 0 9%
Citric Acid USP/NF 0 00007% - 0 02%
Sterile Water for Injection USP Q S 100% pH of the solution 4 0 - 8 0
(Adjust pH using 0 1 N HCI or 0 1 N NaOH) Example 11 Urea USP/NF 001% -200% Isopropyl Alcohol (90%) 05% - 20% Sterile Water for Injection USP QS 100% pH ofthe solution 40 - 80
(Adjust pH using 01N HCI or 01N NaOH)
Example 12
Urea USP/NF 001% -200%
Isopropyl Alcohol (90%) 05% - 20%
Propylene Glycol 010% -500%
Citric Acid USP/NF 000007% - 002%
Sterile Water for Injection USP QS 100% pH ofthe solution 40 - 80
(Adjust pH using 01 N HCI or 01 N NaOH)
Example 13
Urea USP/NF 001% -200%
Propylene Glycol 010% -500%
Citric Acid USP/NF 000007% - 002%
Sterile Water for Injection USP QS 100% pH of the solution 40 - 80
(Adjust pH using 01N HCI or 01N NaOH)
Example 14 Urea USP/NF 001% -200% Polyethylene Glycol 010% -500% Sodium Chloride USP/NF 010% -090% Sterile Water for Injection USP QS 100% pH ofthe solution 40 - 80 (Adjust pH using 01 N HCI or 01 N NaOH)
Examples of Aqueous Hydroxyurea Solutions
The following are examples of hydroxyurea-contaming formulations useable in accordance with the present invention Example 15
Hydroxyurea USP/NF 40%
Sodium Chloride USP/NF 010% -090%
Citric Acid USP/NF 000007% - 002%
Sterile Water for Injection USP QS 100% pH of the solution 40 - 80
(Adjust pH using 01 HCI or 01 N NaOH)
Example 16 Hydroxyurea USP/NF 40% Sodium Chloride USP/NF 010% -090% Sterile Water for Injection USP QS 100% pH of the solution 40 - 80
(Adjust pH using 01 N HCI or 01 N NaOH)
Example 17 Hydroxyurea USP/NF 001% -150% Sodium Chloride USP/NF 010% -090% Sterile Water for Injection USP QS 100% pH ofthe solution 40 - 80
(Adjust pH using 01 N HCI or 01 N NaOH)
Example 18
Hydroxyurea USP/NF 40%
Potassium Phosphate Dibasic USP/NF 50 - 500 milhmolar
Sterile Water for Injection USP QS 100% pH ofthe solution 40 - 80
(Adjust pH using 01 N HCI or 01 N NaOH)
Example 19
Hydroxyurea USP/NF 40%
Sorbitol USP/NF 010% -050%
Citric Acid USP/NF 000007% - 002%
Sterile Water for Injection USP QS 100% pH of the solution 40 - 80
(Adjust pH using 01 N HCI or 01 N NaOH)
Examples of Aqueous Antimetabolite Formulations
The following are examples of formulations for Antimetabohte solutions that are usable in accordance with the present invention To treat a patient's eye with the a combination of antimetabohte and urea or another agent of the present invention, these antimetabohte soulitions may be combined with the aqueous solution of urea or other agent or the antimetabohte solution may be administered topically or injected separate from the aqueous solution of urea or other agent
Example 20
Hydroxyurea USP/NF 001% -150%
Sodium Chloride USP/NF 010% -090%
Citric Acid USP/NF 000007% - 002%
Sterile Water for Injection USP QS 100% pH ofthe solution 40 - 80
(Adjust pH using 01 N HCI or 01 N NaOH)
Example 21
Mitomycm C 100μg-200 mg
Sodium Chloride USP/NF 010% -090%
Citric Acid USP/NF 000007% - 002%
Sterile Water for Injection USP QS 100% pH ofthe solution 40 - 80
(Adjust pH using 01N HCI or 01N NaOH)
Example 22
Thiourea 0010% -100%
Sodium Chloride USP/NF 010% -090%
Citric Acid USP/NF 000007% - 002%
Sterile Water for Injection USP QS 100% pH ofthe solution 40 - 80
(Adjust pH using 01 N HCI or 01 N NaOH)
Example 23
Thiourea 0010% -100%
Sterile Water for Injection USP QS 100% pH ofthe solution 40 - 80
(Adjust pH using 01 N HCI or 01 N NaOH)

Claims

What is claimed is 1 A method for treating a disorder of the eye of a human or veterinary patient, said method comprising the step of
A delivering by topical application or by intrastromal, sub-conjunctival injection of the eye an aqueous solution containing a therapeutically effective amount of an agent selected from the group consisting of, urea, urea derivatives, non-enzymatic protein urea, non-enzymatic proteins, nucleosides, nucleotides and their derivatives, adenine, adenosine, cytosine, cytadine, guanine, guanitadine, guanidine, guanidinium chloride, guanidinium salts, thymidine, thymitadme, uradine, uracil, cysteme, reduced thioctic acid, uric acid, calcium acetyl sahcylate, ammonium sulfate and compounds capable of causing non enzymatic dissolution proteoglycans
2 A method according to Claim 1 wherein said agent is delivered to the cornea by topical application
3 A method according to Claim 1 wherein said agent is delivered to the cornea by intrastromal injection, injection into the Anterior chamber and sub- conjunctival injection
4 A method according to Claim 1 wherein the agent delivered in Step A contains a urea derivative selected from the group consisting of hydroxyurea, thiourea, and, possible combinations thereof 5 A method according to Claim 1 wherein the agent delivered in Step A is an agent capable of causing at least one of de-epithi ahzation of the cornea, softening of the cornea, compression of the collagen fibril packing, treatment of pterigiums, treatment of corneal Neovascularization, treatment of ins Neovascularization and the treatment of glaucoma, said agent being selected from the group consisting of, urea, urea derivatives, hydroxyurea, thiourea, nucleosides, nucleotides, adenine, adenosine, cytosine, cytadine, guanine, guanidine, guanidinium chloride, guanidinium salts, guanitadine, thymidine, thymitadme, uradine, uracil, cysteme, reduced thioctic acid, uric acid, calcium acetyl sahcylate, ammonium sulfate, isopropyl alcohol, ethyl alcohol, polyethylene glycol, polypropylene glycol, and poloxamer block polymers
6 A method according to Claim 1 wherein the agent delivered in Step A comprises 30 micrograms - 7500 micrograms of urea per 50mιcrohters to 100 UL of solution
7 A method according to Claim 1 wherein the solution delivered in Step A comprises approximately 300 micrograms of urea per 50 microhters of solution
8 A method according to Claim 1 wherein Step A delivers a dose of 0 01 % to 15 0% urea onto the cornea of the eye 9 A method according to Claim 1 wherein the solution delivered in Step A comprises a solution that contains at least one agent selected from the group consisting of urea, hydroxyurea, thiourea, mitomicyn C, Polyethylene Glycol, Polypropylene Glycol, and, Poloxamer
10 A method according to Claim 1 wherein Step A comprises delivering into the anterior segment of the eye a therapeutically effective amount of a solution comprising i) urea and/or urea derivatives or mixtures thereof and n) at least one antimetabohte agent
1 1 A method according to Claim 1 wherein Step A comprises delivering into the anterior segment of the eye a therapeutically effective amount of a solution comprising i) urea and/or urea derivatives or mixtures thereof and n) at least one polyglycol agent
12 A method according to Claim 1 wherein the agent is an antimetabohte selected from the group consisting of mitomicyn C, methotrexate,
6-mercaptopurιne, thioguanine,
5-fluorouracιl, cytosine arabmoside,
5-azacytιdιne, hydroxyurea, thiourea, and, possible combinations thereof
13 A method according to Claim 1 wherein the agent delivered in Step A comprises 2000 micrograms of urea and 2000 micrograms of antimetabohte hydroxyurea, or 5 0 microgram of Mitomycm C per 50 microhters of solution
14 A method according to Claim 1 wherein the agent delivered in Step A comprises approximately 300 micrograms of urea and approximately 2000 micrograms of hydroxyurea, or 10 micrograms mitomicyn C per 50 microhters of solution
15 A method according to Claim 1 wherein Step A is repeated a plurality of times and each performance of Step A delivers a dose of 2000 micrograms urea and a dose of 5 0 micrograms of an anti-metabolite mitomicyn C or an antimetabohte
16 A method according to Claim 1 wherein the method is performed for a purpose selected from the group consisting of
causing a non-toxic mechanical/chemical de-epithehahzation of the corneal epithelium for refractive LASEK correction,
causing a non-toxic dissolution of the corneal proteoglycans and interface closure and organized healing of corneal stroma in refractive LASIK correction,
causing a non-toxic dissolution of other proteins and ammo acids for compressing the collagen fibrils for better visual acuity and better quality of vision,
causing the softening of the cornea for the non-surgical refractive correction of myopia, Presbyopia, hyperopia, astigmatism and keratoconus, and applying a contact lens to the softened cornea,
causing dissolution of newly synthesized proteoglycans that are responsible for corneal haze and corneal Opacification, causing dissolution of proteoglycans in the anterior chamber that are responsible for decreased of mtra-ocular pressure by increasing outflow and treatment of glaucoma
causing a solvent action on fibroblasts, inhibiting fibroblasts, inhibiting or preventing corneal fibrosis and scar formation, inhibiting the proliferation of fibroblasts in ocular tissue, and inhibiting VEGF activity in the cornea and the ins, by virtue of its anti-angiogenic effect, thus eliminating both the progression and the regression of corneal new vessels and iris new vessels
17 A method according to Claim 1 wherein said agent is delivered into the anterior segment of the eye by topical application, by intrastromal injection and by subconjunctival injection by initially administering the agent to the anterior segment of the eye in a form and dose that is sufficient to cause a therapeutic amount of the agent to distribute to the anterior segment
18 The use of a compound selected from the group consisting urea, urea derivatives, non-enzymatic protein urea, non-enzymatic proteins, nucleosides, nucleotides and their derivatives, adenine, adenosine, cytosine, cytadine, guanine, guanitadine, guanidine, guanidinium chloride, guanidinium salts, thymidine, thymitadme, uradine, uracil, cysteme, reduced thioctic acid, uric acid, calcium acetyl sahcylate ammonium sulfate and compounds capable of causing non enzymatic dissolution proteoglycans in the preparation of an aqueous solution for delivery by topical application to the eye or by intrastromal or sub-conjunctival injection into the eye for treatment of a disorder of the eye
EP03711552A 2002-03-14 2003-03-14 Agents for corneal or intrastromal administration to treat or prevent disorders of the eye Withdrawn EP1482922A4 (en)

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US20030199574A1 (en) 2000-03-02 2003-10-23 Vitreo-Retinal Technologies, Inc. Treatment of ophthalmic disorders using urea and urea derivatives
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Citations (2)

* Cited by examiner, † Cited by third party
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
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Title
See also references of WO03077898A1 *

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