EP1463799A2 - Procedes d'induction de lymphocytes t regulateurs professionnels et des lymphocytes t produisant des cytokines - Google Patents
Procedes d'induction de lymphocytes t regulateurs professionnels et des lymphocytes t produisant des cytokinesInfo
- Publication number
- EP1463799A2 EP1463799A2 EP02799306A EP02799306A EP1463799A2 EP 1463799 A2 EP1463799 A2 EP 1463799A2 EP 02799306 A EP02799306 A EP 02799306A EP 02799306 A EP02799306 A EP 02799306A EP 1463799 A2 EP1463799 A2 EP 1463799A2
- Authority
- EP
- European Patent Office
- Prior art keywords
- cells
- regulatory
- cell
- population
- tgf
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
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- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K2035/122—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells for inducing tolerance or supression of immune responses
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- C12N2501/20—Cytokines; Chemokines
- C12N2501/23—Interleukins [IL]
Definitions
- the present inventions provides methods for inhibiting aberrant or undesirable immune responses comprising administering the regulatory T cells generated using the regulatory compositions described herein.
- activated professional regulatory T cells When activated professional regulatory T cells are mixed with conventional T cells, they generate a population of cytokine producing regulatory T cells when treated with a T cell activation ( Figure 1 B). Significantly, repeated treatment with a regulatory composition comprising cytokines is not needed to generate this population of cytokine producing regulatory T cells.
- the memory-effector subset which contains the vast majority of cells capable of responding to recall antigens and can be generated in vitro from the aforementioned na ⁇ ve subset following appropriate activation, predominantly expresses the low molecular weight, high activity, RO CD45 isoform and shows efficient effector function (e.g., production of effector cytokines, cytotoxicity), increased CD95/FAS expression, and increased susceptibility to apoptosis, high levels of CD11a/CD18 and the other general adhesion molecules listed above, and heterogenous expression of the L-selectin, ⁇ 4 ⁇ 7 integrin, and CD27 (Picker and Siegelman, (1999) "Lymphoid Tissues and Organs" in W.
- T cells are prepared by immediate rosetting with 2-aminoethylisothiouronium bromide-treated SRBC. T cells are further purified from rosetting cells by staining with antibodies (Abs) to CD16, CD74, and CD11b and deleting reactive cells using immunomagnetic beads. The percentage of CD3+ cells in this fraction is usually greater than 96% (see Zheng, et al., (2002) J. Immunol., 169: 4183-4189).
- the kit further comprises at least one dose of an regulatory composition.
- Dose in this context means an amount of the regulatory composition such as cytokines, that is sufficient to cause an effect. In some cases, multiple doses may be included.
- the dose may be added to the cell treatment container using a port; alternatively, in a preferred embodiment, the dose is already present in the cell treatment container.
- the dose is in a lyophilized form for stability, that can be reconstituted using the cell media, or other reagents.
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Abstract
Cette invention porte, de manière générale, sur des procédés utilisés dans l'induction des lymphocytes T ayant une activité suppressive. Ces procédés sont plus particulièrement utilisés pour générer des lymphocytes T régulateurs professionnels et des lymphocytes T produisant des cytokines et dont l'activité suppressive est améliorée.
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US34265501P | 2001-12-21 | 2001-12-21 | |
US342655P | 2001-12-21 | ||
US37410202P | 2002-04-19 | 2002-04-19 | |
US374102P | 2002-04-19 | ||
PCT/US2002/041288 WO2003059264A2 (fr) | 2001-12-21 | 2002-12-20 | Procedes d'induction de lymphocytes t regulateurs professionnels et des lymphocytes t produisant des cytokines |
Publications (2)
Publication Number | Publication Date |
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EP1463799A2 true EP1463799A2 (fr) | 2004-10-06 |
EP1463799A4 EP1463799A4 (fr) | 2006-01-18 |
Family
ID=26993124
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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EP02799306A Withdrawn EP1463799A4 (fr) | 2001-12-21 | 2002-12-20 | Procedes d'induction de lymphocytes t regulateurs professionnels et des lymphocytes t produisant des cytokines |
Country Status (5)
Country | Link |
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EP (1) | EP1463799A4 (fr) |
JP (1) | JP2005514054A (fr) |
AU (1) | AU2002364230B2 (fr) |
CA (1) | CA2469800A1 (fr) |
WO (1) | WO2003059264A2 (fr) |
Families Citing this family (4)
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EP1715034A4 (fr) * | 2004-02-12 | 2007-11-21 | Immunofrontier Inc | Cellulle r gulatrice controllant l' activite immunologique d' une cellule thymodependante |
EP1812563B1 (fr) | 2004-10-29 | 2013-04-10 | Benaroya Research Institute at Virginia Mason | Méthodes de génération de cellules t de régulation cd4+cd25+ spécifiques à un antigène, compositions et méthodes d'utilisation associées |
US8852581B2 (en) * | 2005-08-31 | 2014-10-07 | Ith Immune Therapy Holdings Ab | Treatment of inflammatory bowel disease |
ITRM20070437A1 (it) * | 2007-08-10 | 2009-02-11 | Istituto Naz Per Le Malattie I | Metodo per la generazione ed espansione di cellule t gamma/delta regolatorie cellule cosi' ottenute e loro impieghi |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2001077299A2 (fr) * | 2000-04-11 | 2001-10-18 | University Of Southern California | Methode de prevention de rejet de greffe par utilisation de tgf-$g(b) pour induire des cellules t suppresseurs |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5374549A (en) * | 1991-01-31 | 1994-12-20 | Terumo Corporation | Process of enriching adherent CD4+ T cells from monocyte depleted peripheral blood mononuclear cells with interleukin 2 and interleukin 4 |
PT1028738E (pt) * | 1997-11-05 | 2003-08-29 | Univ Southern California | Utilizacao de citoquinas e mitogenios para inibir respostas imunitarias patologicas |
ATE332963T1 (de) * | 1999-01-22 | 2006-08-15 | Schepens Eye Res Inst | Aktivierung von regulatorischen t zellen durch ein alpha-melanocyten stimulierendes hormon |
US6746670B2 (en) * | 2000-08-15 | 2004-06-08 | Schering Corporation | Regulatory T cells; methods |
EP1241249A1 (fr) * | 2001-03-12 | 2002-09-18 | Gerold Schuler | Lymphocytes T CD4+CD25+ régulateurs provenant du sang humain |
FR2824567B1 (fr) * | 2001-05-11 | 2003-08-08 | Inst Nat Sante Rech Med | Procede d'obtention de lymphocytes tr1 regulateurs specifiques d'antigene |
-
2002
- 2002-12-20 AU AU2002364230A patent/AU2002364230B2/en not_active Ceased
- 2002-12-20 CA CA002469800A patent/CA2469800A1/fr not_active Abandoned
- 2002-12-20 WO PCT/US2002/041288 patent/WO2003059264A2/fr active Application Filing
- 2002-12-20 EP EP02799306A patent/EP1463799A4/fr not_active Withdrawn
- 2002-12-20 JP JP2003559429A patent/JP2005514054A/ja active Pending
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2001077299A2 (fr) * | 2000-04-11 | 2001-10-18 | University Of Southern California | Methode de prevention de rejet de greffe par utilisation de tgf-$g(b) pour induire des cellules t suppresseurs |
Non-Patent Citations (4)
Title |
---|
HORWITZ D A ET AL: "Role of NK cells and TGF-beta in the regulation of T-cell-dependent antibody production in health and autoimmune disease." MICROBES AND INFECTION / INSTITUT PASTEUR. DEC 1999, vol. 1, no. 15, December 1999 (1999-12), pages 1305-1311, XP002355571 ISSN: 1286-4579 * |
See also references of WO03059264A2 * |
STEPHENS L A ET AL: "Human CD4(+)CD25(+) thymocytes and peripheral T cells have immune suppressive activity in vitro." EUROPEAN JOURNAL OF IMMUNOLOGY. APR 2001, vol. 31, no. 4, April 2001 (2001-04), pages 1247-1254, XP002210570 ISSN: 0014-2980 * |
ZHENG SONG GUO ET AL: "Generation ex vivo of TGF-beta-producing regulatory T cells from CD4+CD25- precursors." JOURNAL OF IMMUNOLOGY (BALTIMORE, MD. : 1950) 15 OCT 2002, vol. 169, no. 8, 15 October 2002 (2002-10-15), pages 4183-4189, XP002355572 ISSN: 0022-1767 * |
Also Published As
Publication number | Publication date |
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EP1463799A4 (fr) | 2006-01-18 |
CA2469800A1 (fr) | 2003-07-24 |
AU2002364230A1 (en) | 2003-07-30 |
WO2003059264A3 (fr) | 2004-02-12 |
AU2002364230B2 (en) | 2008-05-01 |
WO2003059264A9 (fr) | 2004-11-11 |
WO2003059264A2 (fr) | 2003-07-24 |
JP2005514054A (ja) | 2005-05-19 |
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