EP1445326A3 - Practical in vitro sialylation of recombinant glycoproteins - Google Patents

Practical in vitro sialylation of recombinant glycoproteins Download PDF

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Publication number
EP1445326A3
EP1445326A3 EP04075546A EP04075546A EP1445326A3 EP 1445326 A3 EP1445326 A3 EP 1445326A3 EP 04075546 A EP04075546 A EP 04075546A EP 04075546 A EP04075546 A EP 04075546A EP 1445326 A3 EP1445326 A3 EP 1445326A3
Authority
EP
European Patent Office
Prior art keywords
practical
recombinant glycoproteins
sialylation
vitro sialylation
glycoproteins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP04075546A
Other languages
German (de)
French (fr)
Other versions
EP1445326A2 (en
Inventor
James C. Paulson
Robert J. Bayer
Eric Sjoberg
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novo Nordisk AS
Original Assignee
Neose Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Neose Technologies Inc filed Critical Neose Technologies Inc
Priority claimed from EP98904583A external-priority patent/EP1015622B1/en
Publication of EP1445326A2 publication Critical patent/EP1445326A2/en
Publication of EP1445326A3 publication Critical patent/EP1445326A3/en
Withdrawn legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

This invention provides methods for practical in vitro sialylation of glycoproteins, including recombinantly produced glycoproteins. The methods are useful for large-scale modification of sialylation patterns.
EP04075546A 1997-01-16 1998-01-15 Practical in vitro sialylation of recombinant glycoproteins Withdrawn EP1445326A3 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US3571097P 1997-01-16 1997-01-16
EP98904583A EP1015622B1 (en) 1997-01-16 1998-01-15 Practical in vitro sialylation of recombinant glycoproteins
US35710 1998-03-05

Related Parent Applications (2)

Application Number Title Priority Date Filing Date
EP98904583A Division EP1015622B1 (en) 1997-01-16 1998-01-15 Practical in vitro sialylation of recombinant glycoproteins
EP98904583.6 Division 1998-07-23

Publications (2)

Publication Number Publication Date
EP1445326A2 EP1445326A2 (en) 2004-08-11
EP1445326A3 true EP1445326A3 (en) 2007-07-11

Family

ID=32658255

Family Applications (1)

Application Number Title Priority Date Filing Date
EP04075546A Withdrawn EP1445326A3 (en) 1997-01-16 1998-01-15 Practical in vitro sialylation of recombinant glycoproteins

Country Status (1)

Country Link
EP (1) EP1445326A3 (en)

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
HIGA H H ET AL: "SIALYLATION OF GLYCOPROTEIN OLIGOSACCHARIDES WITH N ACETYLDIACETYLNEURAMINIC-ACID N GLYCOLYLDIACETYLNEURAMINIC-ACID AND N-O DIACETYLNEURAMINIC-ACIDS", JOURNAL OF BIOLOGICAL CHEMISTRY. (MICROFILMS), AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 260, no. 15, 25 July 1985 (1985-07-25), pages 8838 - 8849, XP002195701 *
KITAGAWA H ET AL: "CLONING OF A NOVEL ALPHA2,3-SIALYLTRANSFERASE THAT SIALYLATES GLYCOPROTEIN AND GLYCOLIPID CARBOHYDRATE GROUPS", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOCHEMICAL BIOLOGISTS, BIRMINGHAM,, US, vol. 269, no. 2, 14 January 1994 (1994-01-14), pages 1394 - 1401, XP002013111, ISSN: 0021-9258 *
PAULSON J C ET AL: "ENZYMATIC PROPERTIES OF BETA-D GALACTOSIDE ALPHA-2 TO 6 SIALYL TRANSFERASE FROM BOVINE COLOSTRUM", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 252, no. 7, 1977, pages 2363 - 2371, XP002432400, ISSN: 0021-9258 *
WEINSTEIN J ET AL: "SIALYLATION OF GLYCO PROTEIN OLIGO SACCHARIDES N LINKED TO ASPARAGINE ENZYMATIC CHARACTERIZATION OF A GALACTOSYL-BETA-1-34-N-ACETYLGLUCOSAM INYL-ALPHA-2-3 SIALYL TRANSFERASE AND A GALACTOSYL-BETA-1-4-N-ACETYLG LUCOSAMINYL-ALPHA-2-6 SIALYL TRANSFERASE FROM RAT LIVER", JOURNAL OF BIOLOGICAL CHEMISTRY. (MICROFILMS), AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 257, no. 22, 25 November 1982 (1982-11-25), pages 13845 - 13853, XP002195702 *
WILLIAMS M A ET AL: "LARGE-SCALE EXPRESSION OF RECOMBINANT SIALYLTRANSFERASES AND COMPARISON OF THEIR KINETIC PROPERTIES WITH NATIVE ENZYMES", GLYCOCONJUGATE JOURNAL, CHAPMAN & HALL, GB, vol. 12, no. 6, December 1995 (1995-12-01), pages 755 - 761, XP002913437, ISSN: 0282-0080 *

Also Published As

Publication number Publication date
EP1445326A2 (en) 2004-08-11

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