EP1444524A2 - Procedes pour identifier des modulateurs de l'expression ou de l'activite de la chelatase de magnesium dans les plantes - Google Patents

Procedes pour identifier des modulateurs de l'expression ou de l'activite de la chelatase de magnesium dans les plantes

Info

Publication number
EP1444524A2
EP1444524A2 EP01970987A EP01970987A EP1444524A2 EP 1444524 A2 EP1444524 A2 EP 1444524A2 EP 01970987 A EP01970987 A EP 01970987A EP 01970987 A EP01970987 A EP 01970987A EP 1444524 A2 EP1444524 A2 EP 1444524A2
Authority
EP
European Patent Office
Prior art keywords
chelatase
plant
arabidopsis
compound
chl
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP01970987A
Other languages
German (de)
English (en)
Inventor
Jeffrey P. Woessner
Adel Zayed
Jorn Gorlach
Douglas Boyes
Keith Davis
Carol Hamilton
Neil Hoffman
Andreas Kloti
Robert Ascenzi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cogenics Icoria Inc
Original Assignee
Paradigm Genetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Paradigm Genetics Inc filed Critical Paradigm Genetics Inc
Publication of EP1444524A2 publication Critical patent/EP1444524A2/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/25—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value

Definitions

  • the invention relates generally to plant molecular biology.
  • the invention relates to methods for the identification of compounds that regulate plant growth and development through the modulation of magnesium chelatase expression or activity.
  • binding refers to an interaction between Mg-chelatase or a subunit thereof, and a molecule or compound, wherein the interaction is dependent upon the primary amino acid sequence or the conformation of Mg-chelatase and/or the Mg-chelatase subunit.
  • the Mg-chelatases useful in the methods of the invention can be from any species of Arabidopsis.
  • Arabidopsis species include, but are not limited to, Arabidopsis arenosa, Arabidopsis bursifolia, Arabidopsis cebennensis, Arabidopsis croatica, Arabidopsis grtffithiana, Arabidopsis halleri, Arabidopsis himalaica, Arabidopsis korshinskyi, Arabidopsis lyrata, Arabidopsis neglecta, Arabidopsis pumila, Arabidopsis suecica, Arabidopsis thaliana and Arabidopsis wallichii.
  • Mg-chelatase subunits are added to 6 ⁇ M deuteropo ⁇ hyrin IX, 5 mM ATP, 25 mM creatine kinase, 25 MgCl , 2.5 units creatine kinase, 20 mM Tris pH 9.0 in a volume of 50 ⁇ l, and incubated for 20 minutes at 30°C.
  • the reaction is stopped by the addition of 1 ml acetone/water/25% ammonia (80:20: 1 , vol/vol/vol) and 200 ⁇ l hexane.
  • the phases are then separated by brief centrifugation, and the emission spectrum of the bottom acetone phase is determined form 550 to 650 with an excitation wavelength of 408 nm.
  • the driver expression cassette was introduced into Arabidopsis thaliana by agroinfection. Transgenic plants that stably expressed the driver transcription factor were obtained.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Microbiology (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

L'invention concerne la chélatase de magnésium dont il a été découvert qu'elle est essentielle pour la croissance d'Arabidopsis. De manière plus spécifique, l'inhibition de l'expression du gène CHL H de la chélatase de magnésium dans les plants d'Arabidopsis provoque la modification des niveaux de chlorose (jaunissement), une croissance sensiblement réduite et des anomalies de développement. Ainsi, la chélatase de magnésium d'Arabidopsis peut être utilisée comme cible pour l'identification d'herbicides. La présente invention porte aussi sur des procédés pour identifier des composée qui modulent l'expression ou l'activité de la chélatase de magnésium d'Arabidopsis, comprenant les opérations suivantes : mettre en contact un composé avec la chélatase de magnésium d'Arabidopsis ou avec une sous-unité de celle-ci, et déterminer la présence et/ou l'absence de liant entre ledit composé et ladite chélatase de magnésium, ou bien détecter un changement dans l'expression ou l'activité de la chélatase de magnésium. Ces procédés sont utiles pour identifier des herbicides ou d'autres composés susceptibles de moduler la croissance et le développement d'une plante. Lesdits procédés sont en outre utiles pour l'identification de composés qui stimulent l'expression ou la fonction de la chélatase de magnésium. De tels composés peuvent être utilisés pour favoriser ou manipuler la croissance et le développement d'une plante.
EP01970987A 2001-09-14 2001-09-14 Procedes pour identifier des modulateurs de l'expression ou de l'activite de la chelatase de magnesium dans les plantes Withdrawn EP1444524A2 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2001/028761 WO2002022857A2 (fr) 2000-09-12 2001-09-14 Procedes pour identifier des modulateurs de l'expression ou de l'activite de la chelatase de magnesium dans les plantes

Publications (1)

Publication Number Publication Date
EP1444524A2 true EP1444524A2 (fr) 2004-08-11

Family

ID=32653968

Family Applications (1)

Application Number Title Priority Date Filing Date
EP01970987A Withdrawn EP1444524A2 (fr) 2001-09-14 2001-09-14 Procedes pour identifier des modulateurs de l'expression ou de l'activite de la chelatase de magnesium dans les plantes

Country Status (2)

Country Link
EP (1) EP1444524A2 (fr)
WO (1) WO2002022857A2 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7329489B2 (en) 2000-04-14 2008-02-12 Matabolon, Inc. Methods for drug discovery, disease treatment, and diagnosis using metabolomics
EP1285092A4 (fr) 2000-04-14 2003-07-16 Metabolon Inc Procedes permettant de decouvrir des medicaments, de traiter des maladies et de poser des diagnostics a l'aide de la metabolomique
WO2008033575A2 (fr) 2006-09-15 2008-03-20 Metabolon, Inc. Procédés d'identification de cheminements biochimiques

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19717656A1 (de) * 1997-04-25 1998-10-29 Hoechst Schering Agrevo Gmbh DNA-Sequenzen kodierend für die Untereinheit CHLD pflanzlicher Magnesium-Chelatasen, sowie Verfahren zur Aktivitätsbestimmung pflanzlicher Magnesium-Chelatasen
US6570063B1 (en) * 1999-06-04 2003-05-27 E.I. Dupont De Nemours And Company Magnesium chelatase

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO0222857A3 *

Also Published As

Publication number Publication date
WO2002022857A2 (fr) 2002-03-21
WO2002022857A8 (fr) 2004-06-17
WO2002022857A3 (fr) 2003-06-19

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Legal Events

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Ipc: 7C 12Q 1/25 B

Ipc: 7C 12Q 1/00 A

RIN1 Information on inventor provided before grant (corrected)

Inventor name: ASCENZI, ROBERT

Inventor name: KLOTI, ANDREAS

Inventor name: HOFFMAN, NEIL

Inventor name: HAMILTON, CAROL

Inventor name: DAVIS, KEITH

Inventor name: BOYES, DOUGLAS

Inventor name: GORLACH, JORN

Inventor name: ZAYED, ADEL

Inventor name: WOESSNER, JEFFREY, P.

RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: ICORIA, INC.

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