EP1339687A1 - 4-6-diphenyl pyridine derivatives as antiinflammatory agents - Google Patents

4-6-diphenyl pyridine derivatives as antiinflammatory agents

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Publication number
EP1339687A1
EP1339687A1 EP01991731A EP01991731A EP1339687A1 EP 1339687 A1 EP1339687 A1 EP 1339687A1 EP 01991731 A EP01991731 A EP 01991731A EP 01991731 A EP01991731 A EP 01991731A EP 1339687 A1 EP1339687 A1 EP 1339687A1
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European Patent Office
Prior art keywords
amino
alkyl
hydroxyphenyl
cyano
phenyl
Prior art date
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EP01991731A
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German (de)
French (fr)
Other versions
EP1339687B1 (en
Inventor
Toshiki Murata
Sachiko Sakakibara
Takashi Yoshino
Yuka Ikegami
Tsutomu Masuda
Mitsuyuki Shimada
Takuya Shintani
Makoto Shimazaki
Timothy B. Lowinger
Karl B. Ziegelbauer
Kinji Fuchikami
Masaomi Umeda
Hiroshi Komura
Nagahiro Yoshida
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Bayer AG
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Bayer AG
Bayer Healthcare AG
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Publication of EP1339687A1 publication Critical patent/EP1339687A1/en
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Publication of EP1339687B1 publication Critical patent/EP1339687B1/en
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    • C07D213/04—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom
    • C07D213/60—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D213/78—Carbon atoms having three bonds to hetero atoms, with at the most one bond to halogen, e.g. ester or nitrile radicals
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    • C07D213/04—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom
    • C07D213/60—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
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    • C07D213/04—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom
    • C07D213/60—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D213/78—Carbon atoms having three bonds to hetero atoms, with at the most one bond to halogen, e.g. ester or nitrile radicals
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    • C07D405/12—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings linked by a chain containing hetero atoms as chain links

Definitions

  • the present invention relates to novel 4-aryl pyridine derivatives, processes for preparing them and pharmaceutical preparations containing them.
  • the 4-aryl pyridine derivatives of the present invention inhibit MB kinase ⁇ (IKK- ⁇ or IKK- beta) activity, thus inhibit nuclear factor kappa B (NF- ⁇ B) activity, and can be used for the prophylaxis and treatment of diseases associated with NF- ⁇ B activity, in particular for the treatment of inflammatory diseases.
  • Nuclear factor kappa B belongs to a family of closely related homo-and hetero- dimeric transcription factor complexes composed of various combinations of the Rel/NF- ⁇ B family of polypeptides. NF- ⁇ B and related family members are involved in the regulation of more than 50 genes relating to immune and inflammatory responses ((Barnes PJ, Karin M (1997) N Engl J Med 336, 1066-1071) and (Baeuerle PA, Baichwal VR (1997) Adv Immunol 65, 111-137)). In most cell types, NF- ⁇ B is present as a heterodimer comprising a 50 kDa and a 65 kDa subunit (p50/RelA).
  • the heterodimer is sequestered in the cytoplasm in association with inhibitor of NF- ⁇ B (I ⁇ B)-family of proteins to be kept in an inactive state.
  • I ⁇ B-family proteins mask the nuclear translocation signal of NF- ⁇ B.
  • cytokines e.g. TNF- ⁇ , IL-1
  • CD40 ligand e.g. CD40 ligand
  • LPS lipopolysaccharide
  • oxidants e.g. phorbol ester
  • viruses or many others e.g. phorbol ester
  • I ⁇ B proteins are phosphorylated at specific serine residues, poly-ubiquitinated, and then degraded through a proteasome-dependent pathway.
  • the active NF- ⁇ B Freed from I ⁇ B, the active NF- ⁇ B is able to translocate to the nucleus where it binds in a selective manner to preferred gene- specific enhancer sequences.
  • genes being regulated by NF- ⁇ B are many coding for pro-inflammatory mediators, cytokines, cell adhesion molecules, and acute phase proteins. Expression of several of these cytokines and mediators in turn can lead to further activation of NF- ⁇ B via autocrine and paracrine mechanisms.
  • glucocorticoids which are by far the most effective treatment for asthma, inhibit airway inflammation by directly interacting with and inhibiting the activity of the transcription factors NF- ⁇ B and activating peptide- 1 (AP-1) ((Barnes P (1997) Pulmon Pharmacol Therapeut 10, 3-19) and (Dumont A et al. (1998) Trends Biochem Sci 23, 233-235)).
  • NF-B inhibition should improve inflammatory symptoms typical for asthma; allergic rhinitis; atopic dermatitis; hives; conjunctivitis; vernal catarrh; rheumatoid arthritis; systemic lupus erythematosus; psoriasis; diabrotic colitis; systemic inflammatory response syndrome; sepsis; polymyositis; dermatomyositis; Polyaritis nodoa; mixed connective tissue disease; Sjoegren's syndrome; gout, and the like.
  • NF- ⁇ B plays an essential role in neoplastic transformation.
  • NF- ⁇ B is associated with cell transformation in vitro and in vivo as a result of gene overexpression, amplification, rearrangement, or translocation (Mercurio, F., and Manning, A.M. (1999) Oncogene, 18:6163-6171).
  • the genes of NF- ⁇ B family members are rearranged or amplified. Its possible involvement in cancer pathology is also disclosed in Mayo, M.W., Baldwin A.S. (2000) Biochmica et Biophysica Acta 1470 M55-M62. Mayo M.W. et al., discloses the inhibition of NF- ⁇ B results in the blockage the initiation and/or progression of certain cancer, particularly colorectal cancer.
  • NF- ⁇ B may also be involved in the regulation of neuronal cell death. It has been shown that NF- ⁇ B becomes activated and promotes cell death in focal cerebral ischemia (Nature medicine Vol. 5 No. 5, May 1999).
  • IKK I ⁇ B kinase
  • the IKK-complex (molecular weight 700 - 900 kDa) is composed of various proteins including two homologous I ⁇ B kinases, called IKK- ⁇ and IKK- ⁇ , an upstream kinase, NIK which induces NF- ⁇ B, a scaffold protein called IKAP, which tethers together the three kinases, and a regulatory subunit IKK- ⁇ , which preferentially interacts with IKK- ⁇ .
  • IKK- ⁇ is a 756 amino acid serine-threonine kinase showing 52 % identity to and the same domain structure as IKK- ⁇ ((Mercurio F et al. (1997) Science 278, 860-866.),
  • IKK- ⁇ forms homo-dimers and hetero-dimers with IKK- ⁇ in vitro and in cells, respectively. IKK- ⁇ also interacts with IKK- ⁇ , IKAP, NIK and I ⁇ B ⁇ .
  • IKK- ⁇ phosphorylates I ⁇ B ⁇ and I B ⁇ at specific serine residues with equal efficacy (Li I et al. (1998) J Biol Chem 273, 30736-30741).
  • IKK- ⁇ shows a higher constitutive kinase activity as compared to IKK- ⁇ . This is in agreement with data suggesting that over-expression of IKK- ⁇ activates the transcription of a NF- ⁇ B- dependent reporter gene with a higher efficacy as compared to IKK- ⁇ .
  • IKK- ⁇ has been shown to be activated in various cell lines or fresh human cells in response to various stimuli including TNF- ⁇ , IL-l ⁇ , LPS, anti-CD3/anti-CD28 co-stimulation, protein kinase C and calcineurin, B-cell receptor/CD40 ligand stimulation, and vanadate.
  • IKK- ⁇ is activated in f ⁇ broblast-like synoviocytes (FLS) isolated from the synovium of patients suffering from rheumatoid arthritis or osteoarthritis (Kempiak SJ et al. (1999) J Immunol 162, 3176-3187.).
  • FLS f ⁇ broblast-like synoviocytes
  • IKK- ⁇ can be activated by the structurally related upstream kinases MEKK-1 and NIK, most likely through phosphorylation of specific serine residues within the T-loop (activation loop) and by certain protein kinase C isoforms (Lallena MJ et al. (1999) Mol Cell Biol 19, 2180- 2188.).
  • a catalytically inactive mutant of IKK- ⁇ has been shown to inhibit activation of NF- ⁇ B by TNF- ⁇ , IL-l ⁇ , LPS, anti-CD3/anti-CD28 stimulation (Woronicz JD et al. (1997) Science 278, 866-869). The same effects are observed when MEKK1 or NIK are overexpressed.
  • IKK- ⁇ mutations in the activation loop inhibited IL-1 and TNF- ⁇ signaling (Delhase M et al. (1999) Science 284, 309-313.). Based on the experimental results described above, there is clear-cut evidence for a pivotal involvement of IKK- ⁇ in various pathways leading to NF- ⁇ B activation.
  • IKK- ⁇ the specific inhibition of IKK- ⁇ should result in a strong anti- inflammatory and immuno-modulatory effect in vivo with the potential of improving the underlying causes of asthma and other diseases.
  • anti-tumor and anti- ischemic effects of an IKK- inhibitor may be expected.
  • R', R" represent (OCH 3 , OCH 3 ), (CI, CI), (H, CI), (H, Br), (H, CH 3 ), (H, OCH 3 ), (H, NO 2 ), or (H, N(CH 3 ) 2 ),
  • the present inventors have found that the compounds of novel chemical structure related to the present invention have unexpectedly excellent NF- ⁇ B inhibitory activity based on IKK- ⁇ inhibition and cytokine inhibition.
  • the present invention has been accomplished based on these findings.
  • This invention is to provide a novel 4-aryl pyridine derivatives shown by the following formula (I) and salts thereof.
  • X is CH orN
  • R 1 is hydrogen, hydroxy, halogen, - 6 alkyl, alkoxy, Ci- ⁇ alkoxycarbonyl, nitro, amino, mono or di (d. 6 alkyl)amino, phenylsulfonylamino, -NHR 11 or -O-(CH 2 ) n -R 12 ,
  • R ⁇ ⁇ is phenyl substituted Ci- 6 alkyl, C ⁇ - 6 alkanoyl or Ci-fjalkylsulfonyl,
  • n an integer selected from 0 to 6
  • R 12 is C 2 . 6 alkenyl, benzoyl, mono or di phenyl, mono or di alkyl)amino, alkanoyl, Q-e alkoxycarbonyl, or a 3 to 10 membered saturated or unsaturated ring having 0 to 3 heteroatoms selected from the group consisting of S, O and N, and said ring is optionally substituted by hydroxy, nitro, cyano, mono or di halogen, C ⁇ - 6 alkyl, halogen substituted Ci- ⁇ alkyl, amino, mono or di (d- 6 alkyl)amino, alkoxycarbonyl, or phenyl;
  • R 2 is hydrogen, hydroxy, halogen, or d-6 alkyl
  • R 3 is hydrogen, hydroxy, halogen, d- 6 alkoxy, C 3 . 6 cycloalkyl substituted d. 6 alkyloxy, -N R 31 R 32 , or a 3 to 6 membered saturated ring having 0 to 3 heteroatoms selected from the group consisting of O and N, and said ring is optionally substituted by R 33 ;
  • R 31 is hydrogen or d- 6 alkyl
  • R 32 is hydrogen, C ⁇ - 6 alkanoyl, or Cj. 6 alkyl optionally substituted by hydroxy or phenyl,
  • R 33 is nitro, cyano, d- 6 alkyl optionally substituted by hydroxy or amino, C ⁇ - 6 alkoxy, hydroxy substituted - 6 alkyloxy, amino substituted d- 6 alkyloxy, d- 6 alkanoyl, carbamoyl or -NR 33a R 33b
  • R ,33a is hydrogen or d- 6 alkyl
  • R 33b is hydrogen, d- 6 alkyl optionally substituted by hydroxy or phenyl, d- 6 alkanoyl, d. 6 alkylsulfonyl, or trifluoroacetyl,
  • R 4 is hydrogen, hydroxy, carboxy, -CO-NHR 41 , amino, d- 6 alkylsulfonylamino, or -NH-COR 41 , d_ 6 alkyl optionally substituted by R 42 , d- 6 alkoxy, R 43 d- 6 alkyloxy,
  • d-6 alkyl optionally substituted by R 41a , d- 6 alkoxy, oxo- tetrahydrofuryl, oxopyrrolidinyl, -CH(OH)R 41 , -CH(NH 2 )R 41c , -NHR 41c , or piperazino optionally substituted by R 41d ,
  • R 41a is carboxy, d. 6 alkoxy, -CH(NH 2 )carboxy, -NR 1a_1 R 41a"2 , or a
  • R la_1 is hydrogen or C ⁇ - 6 alkyl optionally substituted by hydroxy, C ⁇ _ 6 alkyloxy, C 3 . 8 cycloalkyl, or piperidino,
  • R 41a"2 is hydrogen or d- 6 alkyl optionally substituted by hydroxy, d_ 6 alkyloxy or C 3 _ 6 cycloalkyl, C ⁇ - 6 alkoxy or a 3 to 6 membered saturated ring having 0 to 3 heteroatoms selected from the group consisting of O and N, and said ring is optionally substituted by carboxy, d. 6 alkyl, d_ 6 alkanoyl, or d_ 6 alkyloxy,
  • R 41b is d_ 6 alkyl optionally substituted by carboxy or d_ 6 alkyloxy, Ci. ⁇ 5 alkoxy, or d_ 6 alkoxycarbonyl,
  • R 41c is carboxy, d- 6 alkyl optionally substituted by carboxy or a 3 to 6 membered saturated ring having 0 to 3 heteroatoms selected from the group consisting of O and N, or
  • R 41d is d_ 6 alkyl optionally substituted by carboxy or d- 6 alkyloxy, or d- 6 alkoxy
  • R >42 is d- 6 alkoxy, carboxy, amino, -CH(NH 2 )-carboxy or a 5 to 7 membered saturated or unsaturated ring having 0 to 3 heteroatoms selected from the group consisting of O and N, and said ring is optionally substituted by hydroxy, nitro, mono or di halogen, d- 6 alkyl, halogen substituted d- 6 alkyl, amino, mono or di (d- 6 alkyl)amino, or carbamoyl;
  • R 43 is carboxy, amino, -CH(NH 2 )-carboxy or a 5 to 7 membered saturated or unsaturated ring having 0 to 3 heteroatoms selected from the group consisting of O and N, and said ring is optionally substituted by hydroxy, nitro, mono or di halogen, d- 6 alkyl, halogen substituted d_ 6 alkyl, amino, mono or di (d. 6 alkyl)amino, or carbamoyl;
  • R 3 and R 4 may form, together with the carbon atoms in the benzene ring, a 4 to 6 membered saturated ring having 0 to 3 heteroatoms selected from the group consisting of O and N, and said ring is optionally substituted by one or more substituents selected from the group consisting of hydroxy, nitro, mono or di halogen, d_ 6 alkyl, halogen substituted d-6 alkyl, oxo, amino, mono or di (C ⁇ -6 alkyl)amino, and carbamoyl;
  • R 5 is hydrogen, cyano, carboxy, carbamoyl, d- 6 alkyl optionally substituted by hydroxy or carbamoyl, and d- 6 alkoxycarbonyl, and R° is -NR 61 R 62 .
  • R 61 is hydrogen or d- 6 alkyl
  • R 62 is hydrogen, d_ 6 alkyl, phenyl, benzyl, d. 6 alkanoyl, or
  • NR 61 R 62 may form, together with the nitrogen atom to which they are attached, a saturated 5-6 membered ring optionally containing NH or
  • R 5 and R 6 may form, together with the carbon atoms in the pyridine ring, a 5 to 7 membered saturated or unsaturated ring having 0 to 3 heteroatoms selected from the group consisting of O, S and N, and said ring optionally having substituents selected from the group consisting of halogen, nitro, cyano, oxo, thioxo, d- 6 alkyl, d- 6 alkylsulfonyl, d_ 6 alkoxy, d- 6 alkoxycarbonyl, phenyl, d- 6 alkanoyl, amino, d- 6 alkylamino, d_ 6 alkanoylamino, carbamoyl, C 3 .
  • the compounds of the present invention surprisingly show excellent IKK- ⁇ kinase inhibitory activity and cytokine inhibitory activity. They are, therefore suitable especially as NF- B inhibitors and in particular for the production of pharmaceuticals or pharmaceutical compositions, which may be useful to treat NF- ⁇ B dependent diseases.
  • the 4-aryl pyridine derivatives of the present invention inhibit IKK- ⁇ kinase activity, they are useful for treatment and prophylaxis of diseases involving NF- ⁇ B activity as follows: inflammatory symptoms including asthma; allergic rhinitis; atopic dermatitis; hives; conjunctivitis; vernal catarrh; chronic arthrorheumatism; systemic lupus erythematosus; psoriasis; diabrotic colitis; systemic inflammatory response syndrome (SIRS); sepsis; polymyositis; dermatomyositis (DM); polyarthritis nodosa (PN); mixed connective tissue disease
  • inflammatory symptoms including asthma; allergic rhinitis; atopic dermatitis; hives; conjunctivitis; vernal catarrh; chronic arthrorheumatism; systemic lupus erythematosus; psoriasis; diabro
  • the compounds of the present invention are also useful for treatment and prophylaxis of diseases like ischemia and tumor, since the diseases also relate to IKK- ⁇ kinase and NF- ⁇ B activity.
  • Preferred compounds of the formula (I) are those wherein:
  • X is CH orN
  • R 1 is hydrogen, halogen, C 1 . 6 alkoxy, or cyclopropyl methoxy
  • R 2 is hydrogen
  • R 3 is hydrogen, hydroxy, halogen, amino, mono or di(C ⁇ - 6 alkyl)amino, acetamido, d- 6 alkoxy, cyclopropyl methoxy, d_ 6 alkyl(hydroxy substituted C ⁇ - 6 alkyl)amino, d- 6 alkyl(benzyl)amino, morpholino, piperidino, or pyrrolidino optionally substituted by d- 6 alkyl, hydroxy substituted d_ 6 alkyl, amino, di (d-6 alkyl)amino, or trifluoroacetylamino;
  • R 4 is hydrogen, hydroxy, carboxy, amino, d-6 alkylsulfonylamino, piperazino, d-6 alkyl substituted piperazino, piperidino substituted Cj.6 alkyloxy, piperidino d- 6 alkyl- aminocarbonyl, or-NH-COR 41 ,
  • R 41 is d- 6 alkyl optionally substituted by R 41a , d_6 alkoxy, oxotetrahydrofuryl, oxopyirolidinyl, -CH(OH)R 41 , or -CH(NH 2 )R 41c ,
  • R 41a is C ⁇ -6 alkoxy, carboxy, -CH(NH 2 )-carboxy, -NHR 413"1
  • R 41a_1 is hydrogen, d- 6 alkyl optionally substituted by hydroxy, C 3 . 8 cycloalkyl, d. 6 alkoxy, or piperidino
  • -N(d. 6 alkyl)R 41a'2 wherein R 41a"2 is d- 6 alkyl or d- 6 alkyl substituted pyrrolidinyl), pyrrolidinyl, piperidino optionally substituted by carboxy or d_ 6 alkoxycarbonyl, piperidino fused with cyclohexane, or piperazino optionally substituted by d- 6 alkyl, hydroxy substituted d- 6 alkyl, benzodioxane substituted d_ 6 alkyl, benzyl, C ⁇ - 6 alkanoyl, cyclohexyl, or furoyl,
  • R 41 is C ⁇ -6 alkyl optionally substituted by carboxy or alkoxycarbonyl,
  • R 41c is d- 6 alkyl optionally substituted by carboxy, or
  • R »6 is amino or acetamido
  • R 5 and R° may form -R 50 -CH 2 -NH-, -R 50 -CO-NH-, -R 50 -SO 2 -NH-, or -R
  • R 50 is -CHR 501 -O-, -CH 2 -N R 501 -, -CO-NR 501 -, (wherein R 501 is hydrogen, d- 6 alkyl, d- 6 alkanoyl, d- 6 alkoxycarbonyl, carbamoyl, C ⁇ - 6 alkylsulfonyl, C 3 . 8 cycloalkylaminocarbonyl, d_ 6 alkylaminocarbonyl, di (C ⁇ - 6 alkylaminocarbonyl or phenyl)
  • d- 6 alkyl in general represents a straight-chain or branched hydrocarbon radical having 1 to 6 carbon atoms.
  • Non-limiting examples include methyl, ethyl, propyl, isopropyl, butyl, isobutyl, pentyl, isopentyl, hexyl, isohexyl.
  • C ⁇ - 6 alkoxy in general represents a straight-chain or branched hydrocarbon radical having 1 to 6 carbon atoms and bound via an oxygen atom.
  • Non-limiting examples include methoxy, ethoxy, propoxy, isopropoxy, butoxy, isobutoxy, pentoxy, isopentoxy, hexoxy, isohexoxy.
  • C 3 -s cycloalkyl in general represents a cyclic hydrocarbon radical having 3 to 8 carbon atoms. Cyclopropyl, cyclopentyl and cyclohexyl are preferred.
  • Non-limiting examples include cyclopentyl, cyclohexyl, cycloheptyl and cyclooctyl.
  • C 2 _ 6 alkenyl stands for straight-chain or branched residues containing one or more double bonds, e.g. vinyl, allyl.
  • the preferable compounds of the present invention are as follows:
  • the compound of the formula (I) of the present invention can be, but not limited to be, prepared by combining various known methods.
  • one or more of the substituents, such as amino group, carboxy group, and hydroxy group of the compounds used as starting materials or intermediates are advantageously protected by a protecting group known to a person skilled in the art. Examples of the protecting groups are described in "Protective Groups in Organic Synthesis (2 nd Edition)" by Greene and Wuts.
  • R 41 is the same as defined.
  • reaction A can be prepared, for example, by the following reaction A.
  • R 3 is the same as defined above), malononitrile, and ammonium acetate to prepare the compound (IV).
  • step 2 the nitro group is reduced to convert to the amino group.
  • step 3 the amino group is further modified to obtain a desired group (NHR 4a ).
  • the substituents of the compound (II) or (III) are, if necessary, protected by an appropriate protecting group (e.g., benzyl, silyl) during the reaction, and deprotected afterward.
  • an appropriate protecting group e.g., benzyl, silyl
  • the step 1 is carried out without a solvent or in a solvent including, for instance, ethers, such as dioxane, and tetrahydrofuran; aromatic hydrocarbons such as benzene, toluene and xylene; nitriles such as acetonitrile; amides such as dimethylformamide (DMF) and dimethylacetamide; sulfoxides such as dimethyl sulfoxide, and others.
  • ethers such as dioxane, and tetrahydrofuran
  • aromatic hydrocarbons such as benzene, toluene and xylene
  • nitriles such as acetonitrile
  • amides such as dimethylformamide (DMF) and dimethylacetamide
  • sulfoxides such as dimethyl sulfoxide, and others.
  • the reaction temperature is optionally set depending on the compounds to be reacted.
  • the reaction temperature is usually about 50°C to 200°C.
  • the reaction is conducted for, usually, 30 minutes to 48 hrs. and preferably 1 to 24 hrs.
  • the compounds of (II) and (III) can be commercially available, or can be prepared by the use of known techniques.
  • the step 2 is carried out in the presence of a reducing agent and a hydrogen donor at about room temperature to 120°C.
  • the reaction time and the solvent used in the step 2 are similar as that used in the step 1.
  • the step 3 is carried out under the condition usually employed by a skilled person in the art to synthesize amides (NHR 4a ).
  • X, R 1 , R 2 , R 3 and R 41 are the same as defined above, can be prepared, for example, by the following reaction B.
  • R is the same as defined above, and R 4 is amino or alkoxy), malononitrile, and ammonium acetate to prepare the compound (IV). R 4b is further modified to obtain a desired group (OH or NHR 41 ).
  • the step 1 of the reaction B can be carried out under the similar condition to that of reaction A.
  • the step 2 in the reaction B can be hydrolysis and can be carried out under the condition usually employed by a skilled person in the art.
  • the step 3 in the reaction B can be carried out under the condition usually employed by a skilled person in the art to synthesize amides (CONHR 41 ).
  • R 1 , R 2 , R 3 and R 4 are the same as defined, and R 5a is carboxy, carbamoyl, d- 6 alkoxycarbonyl, hydroxy C ⁇ -6 alkyl, d_ 6 alkylcarbamoyl, or C ⁇ . 6 alkyl,
  • reaction C can be prepared, for example, by the following reaction C.
  • the substituents of the compound of (II) or (III") may be protected by an appropriate protecting group (e.g., benzyl, silyl) during the reaction, and deprotected afterward.
  • an appropriate protecting group e.g., benzyl, silyl
  • the step 1 of the reaction C can be carried out under the similar condition to that of reaction A.
  • the step 2 of the reaction C can be, for example, a hydrolysis reaction, decarboxylation, reducing reaction, amide synthesis, etc.
  • the conditions of the step 2 can be the same as that usually employed by a skilled person in the art.
  • amino group at position 2 of the pyridine ring in the compounds (I-a), (I-b) and (I-c) is, if necessary, modified according to conventional method to prepare other groups such as alkylamino, alcanoylamino, and the like.
  • the starting compound is I-d: wherein X, R 1 , R 2 , R 3 and R 4 are the same as defined above.
  • step 1 of reaction D starting compound of formula I-d in a suitable solvent with suitable organic base can be treated with triphosgene by known method to give a desired compound represented by the formula I-D.
  • starting compound of formula I-d in a suitable solvent can be treated with oxidizing agent such as manganese dioxide, nitric acid and pyridinium chlorochromate (PCC) for preparing a compound represented by the formula I-d'-l.
  • oxidizing agent such as manganese dioxide, nitric acid and pyridinium chlorochromate (PCC) for preparing a compound represented by the formula I-d'-l.
  • a formula I-d'-l in a suitable solvent can be treated with diethylmalonate and suitable base to give a desired compound represented by the formula I-E.
  • starting compound of formula I-d in a suitable solvent can be treated with phosphonic ester reagent such as diphenylphosphoryl azide (DPP A) and suitable base for preparing a compound represented by the formula I-d'-2.
  • phosphonic ester reagent such as diphenylphosphoryl azide (DPP A)
  • suitable base for preparing a compound represented by the formula I-d'-2.
  • compound I-d'-2 can be treated with hydrogen in the presence of a metal catalyst such as palladium on carbon (Pd/C) in a solvent for preparing a compound represented by the formula I-d'-3.
  • a metal catalyst such as palladium on carbon (Pd/C) in a solvent for preparing a compound represented by the formula I-d'-3.
  • step 3 of reaction F compound I-d' -3 in a suitable solvent can be treated with l,l'-carbonyldiimidazole (CDI) to give a desired compound formula I-F-l.
  • CDI l,l'-carbonyldiimidazole
  • compound I-d'-3 can be treated with sulfamide and suitable base to give a desired compound represented by the formula I-F-2.
  • the suitable solvents for the individual steps in the reactions D to F are not limited, but can be selected from the group consisting of ethers, such as dioxane, diethylether, tetrahydrofuran (THF), glycol dimethyl ether, or alcohols such as, propanol, butanol, isopropanol, ethanol diisopropyl ether or esters, such as ethyl acetate, butyl acetate 1, or aromatic hydrocarbons such as benzene, toluene, xylene, hexane or nitriles such as acetonitrile or amides such as dimethylformamide (DMF), dimethylacetamide or sulfoxides such as dimethyl sulfoxide or halogenated hydrocarbons such as dichloromethane, trichloromethane and others. Having described some examples of suitable solvents, the present examples are illustrative and not restrictive.
  • the suitable inorganic or organic bases for the individual steps in the reactions D to F are not limited, but can be selected from the group comprising for instance, such as alkaline earth metal or alkali metal hydroxides, alkoxides, acetates, carbonates or bicarbonates, such as, sodium hydroxide, potassium hydroxide or ammonium hydroxide, sodium methoxide, sodium ethoxide, or potassium tert-butoxide, sodium acetate, potassium acetate, calcium acetate or ammonium acetate, sodium carbonate, potassium carbonate or ammonium carbonate, sodium bicarbonate or potassium bicarbonate and also tertiary amines, such as trimethylamine, triethylamine, tributyl- amine, ethyl-diisopropylamine, N,N-dimethylaniline, N,N-dimethyl-benzylamine, pyridine, picoline, N-methylpiperidine, N-methylmorpholine, N,N-d
  • the reaction temperature is optionally set depending on the compounds to be reacted.
  • the reaction temperature is usually about 0°C to 150°C.
  • the reaction is conducted for, usually, 30 minutes to 48 hrs. and preferably 1 to 24 hrs.
  • Typical salts of the compound shown by the formula (I) include salts prepared by the reaction of the compound of the present invention with a mineral or organic acid, or an organic or inorganic base. Such salts are known as acid addition and base addition salts, respectively.
  • Acids to form acid addition salts include inorganic acids such as, without limitation, sulfuric acid, phosphoric acid, hydrochloric acid, hydrobromic acid, hydroiodic acid and the like, and organic acids, such as, without limitation, p-toluenesulfonic acid, methanesulfonic acid, oxalic acid, p-bromophenylsulfonic acid, carbonic acid, succinic acid, citric acid, benzoic acid, acetic acid, and the like.
  • inorganic acids such as, without limitation, sulfuric acid, phosphoric acid, hydrochloric acid, hydrobromic acid, hydroiodic acid and the like
  • organic acids such as, without limitation, p-toluenesulfonic acid, methanesulfonic acid, oxalic acid, p-bromophenylsulfonic acid, carbonic acid, succinic acid, citric acid, benzoic acid, acetic acid, and the like.
  • Base addition salts include those derived from inorganic bases, such as, without limitation, ammonium hydroxide, alkaline metal hydroxide, alkaline earth metal hydroxides, carbonates, bicarbonates, and the like, and organic bases, such as, without limitation, ethanolamine, triethylamine, tri(hydroxymethyl)aminomethane, and the like.
  • inorganic bases include, sodium hydroxide, potassium hydroxide, potassium carbonate, sodium carbonate, sodium bicarbonate, potassium bicarbonate, calcium hydroxide, calcium carbonate, and the like.
  • salts are sulfate, pyrosulfate, bisulfate, sulfite, bisulfite, phosphate, monohydrogenphosphate, dihydrogenphosphate, metaphosphate, pyrophosphate, chloride, bromide, iodide, acetate, propionate, decanoate, caprylate, acrylate, formate, isobutyrate, caproate, heptanoate, propiolate, oxalate, malonate, succinate, suberate, sevacate, fumarate, maleate, butyne-l,4-dioate, hexyne-l,6-dioate, benzoate, chlorobenzoate, methoxybenzoate, phthalate, sulfonate, xylenesulfonate, phenylacetate, phenylpropionate, phenylbutyrate, citrate, lactate, ⁇ -hydroxybutyrate, glycoll
  • the preferred acid addition salts are those formed with mineral acids, such as, without limitation, hydrochloric acid, and hydrobromic acid, and those formed with organic acids, such as without limitation, maleic acid and methanesulfonic acid.
  • the potassium and sodium salt forms are particularly preferred base addition salts.
  • the compound of the present invention may be administered in oral forms, such as, without limitation normal and enteric coated tablets, capsules, pills, powders, granules, elixirs, tinctures, solution, suspensions, syrups, solid or liquid aerosols and emulsions. They may also be administered in parenteral forms, such as, without limitation, intravenous, intraperitoneal, subcutaneous, intramuscular, and the like forms, well-known to an ordinary skilled person in the pharmaceutical arts.
  • the compounds of the present invention can be administered in intranasal form via topical use of suitable intranasal vehicles, or from transdermal routes, using transdermal delivery systems well-known to an ordinary skilled person in the art.
  • the dosage regimen with the use of the compounds of the present invention is selected by one of ordinary skill in the arts, in view of a variety of factors, including, without limitation, age, weight, sex, and medical condition of the recipient, the severity of the condition to be treated, the route of administration, the level of metabolic and excretory function of the recipient, the dosage form employed, the particular compound and salt thereof employed.
  • the compounds of the present invention are preferably formulated prior to administration together with one or more pharmaceutically acceptable excipients.
  • Excipients are inert substances such as, without limitation carriers, diluents, flavoring agents, sweeteners, lubricants, solubilizers, suspending agents, binders, tablet disintegrating agents and encapsulating material.
  • Another embodiment of the present invention is a pharmaceutical formulation comprising the compound of the invention and one or more pharmaceutically acceptable excipients that are compatible with the other ingredients of the formulation and not deleterious to the recipient thereof.
  • Pharmaceutical formulations of the present invention are prepared by combining a therapeutically effective amount of the compound of the invention together with one or more pharmaceutically acceptable excipients therefor.
  • the active ingredient may be mixed with a diluent, or enclosed within a carrier, which may be in the form of a capsule, sachet, paper, or other container.
  • the carrier may serve as a diluent, which may be solid, semi-solid, or liquid material which acts as a vehicle, or can be in the form of tablets, pills, powders, lozenges, elixirs, suspensions, emulsions, solutions, syrups, aerosols, ointments, containing, for example, up to 10% by weight of the active compound, soft and hard gelatin capsules, suppositories, sterile injectable solutions and sterile packaged powders.
  • the active ingredient may be combined with non-toxic, pharmaceutically acceptable carriers, such as, without limitation, lactose, starch, sucrose, glucose, sodium carbonate, mannitol, sorbitol, calcium carbonate, calcium phosphate, calcium sulfate, methyl cellulose, and the like; together with, optionally, disintegrating agents, such as, without limitation, maize, starch, methyl cellulose, agar bentonite, xanthan gum, alginic acid, and the like; and optionally, binding agents, for example, without limitation, gelatin, acacia, natural sugars, beta-lactose, corn sweeteners, natural and synthetic gums, tragacanth, sodium alginate, carboxy- methyl cellulose, polyethylene glycol, waxes, and the like; and, optionally, lubricants, for example, without limitation, magnesium stearate, sodium stearate, stearic acid, sodium oleate, sodium benzoate, sodium acetate
  • the carrier may be a finely divided solid which is in admixture with the finely divided active ingredient.
  • the active ingredient may be mixed with a carrier having binding properties in suitable proportions and compacted in the shape and size desired to produce tablets.
  • the powders and tablets preferably contain from about 1 to about 99 weight percent of the active ingredient which is the novel composition of the present invention.
  • Suitable solid carriers are magnesium carboxymethyl cellulose, low melting waxes, and cocoa butter.
  • Sterile liquid formulations include suspensions, emulsions, syrups and elixirs.
  • the active ingredient can be dissolved or suspended in a pharmaceutically acceptable carrier, such as sterile water, sterile organic solvent, or a mixture of both sterile water and a sterile organic solvent.
  • the active ingredient can also be dissolved in a suitable organic solvent, for example, aqueous propylene glycol.
  • a suitable organic solvent for example, aqueous propylene glycol.
  • Other compositions can be made by dispersing the finely divided active ingredient in aqueous starch or sodium carboxymethyl cellulose solution or in a suitable oil.
  • the formulation may be in unit dosage form, which is a physically discrete unit containing a unit dose, suitable for administration in human or other mammals.
  • a unit dosage form can be a capsule or tablets, or a number of capsules or tablets.
  • a "unit dose" is a predetermined quantity of the active compound of the present invention, calculated to produce the desired therapeutic effect, in association with one or more excipients.
  • the quantity of active ingredient in a unit dose may be varied or adjusted from about 0.1 to about 1000 milligrams or more according to the particular treatment involved.
  • Typical oral dosages of the present invention when used for the indicated effects, will range from about O.Olmg kg/day to about lOOmg/kg/day, preferably from 0.1 mg/kg/day to 30 mg/kg/day, and most preferably from about 0.5 mg/kg/day to about 10 mg/kg/day.
  • parenteral administration it has generally proven advantageous to administer quantities of about 0.001 to 100 mg/kg/day, preferably from 0.01 mg kg/day to lmg/kg/day.
  • the compounds of the present invention may be administered in a single daily dose, or the total daily dose may be administered in divided doses, two, three, or more times per day. Where delivery is via transdermal forms, of course, administration is continuous.
  • a cDNA fragment encoding human IKK- ⁇ open reading frame was generated by PCR with the use of a pair of primers designed from the published sequence (Woronicz JD et al. (1997) Science 278,866-869).
  • a template was obtained from Quickclone cDNA (Clontech) using ElongaseTM Amplification kit (Life Technologies).
  • the DNA fragments generated by PCR were gel- purified and subcloned into pBluescript.
  • the cDNA fragment cloned in pBluescript was inserted into pcDNA3.1/His C Kpnl/Notl, and transferred into pNL1393 Smal/Xbal (Pharmingen) to construct a baculovirus transfer vector.
  • An expression vector containing the nucleotide sequence encoding fusion protein of GST with amino acid residues 1 to 54 of IKBOC under the control of an IPTG-inducible promoter was constructed.
  • the expression vector was introduced in E. coli and the transformant was cultured and lysed to obtain a GST-I ⁇ B ⁇ fusion protein. Then the resulting GST-I ⁇ B ⁇ fusion protein was purified and biotinated for kinase assay.
  • the 96-well format kinase assay of IKK- ⁇ was performed to test the inhibitory activity of the compounds of the present invention.
  • 5 ⁇ l of a test compound was put in the presence of 2.5% dimethyl sulfoxide (DMSO) in each well in a U-bottomed 96-well plate (Falcon).
  • DMSO dimethyl sulfoxide
  • TP total phosphorylation
  • 5 ⁇ l of 2.5% DMSO was put.
  • Recombinant IKK- ⁇ (final 0.6 ⁇ g/ml) and bio-GST-I ⁇ B ⁇ (1-54) (final 0.2 ⁇ M) were diluted in 25 ⁇ l of 2 x kinase buffer ⁇ (40mM Tris-HCl, pH 7.6,
  • Biotechniche Analysen GmbH #08114E14.FWD Biotechniche Analysen GmbH #08114E14.FWD
  • non-bound radioactivity was eliminated by washing the wells five times with 300 ⁇ l of washing buffer including 0.9% NaCl and 0.1 % (w/v) Tween-20 with the use of a MW-96 plate washer (BioTec).
  • the bound radioactivity was determined after the addition of 170 ⁇ l MicroScint-PS scintillation cocktail (Packard) using a TopCount scintillation counter.
  • a cDNA fragment encoding human Syk openreading frame was cloned from total RNA of human Burkitt's lymphoma B cell lines, Raji (American Type Culture Collection), with the use of RT-PCR method.
  • the cDNA fragment was inserted into pAcG2T (Pharmingen, San Diego, CA) to construct a baculovirus transfer vector. Then the vector, together with the linearized baculovirus (BaculoGoldTM, Pharmingen), was used to transfect Sf21 cells (Invifrogen, San Diego, CA).
  • Sf21 cells were infected with this amplified high titer virus to produce a chimeric protein of Syk kinase fused by glutathione-S-transferase (GST).
  • GST glutathione-S-transferase
  • the resulting GST-Syk was purified with the use of glutathione column (Amersham Pharmacia Biotech AB, Uppsala, Sweden) according to the manufacturer's instruction. The purity of the protein was confirmed to be more than 90% by SDS-PAGE.
  • a peptide fragment of 30 residues including two tyrosine residues was synthesized by a peptide synthesizer.
  • the N-terminus of the fragment was then biotinylated to obtain biotinylated activation loop peptide (AL).
  • the mixture was incubated for 1 hr. at room temperature, and the reaction was terminated by the addition of 120 ⁇ l of termination buffer (50 mM Tris-
  • Tris-buffered saline 50mM Tris-HCl (pH 8.0), 138mM NaCl, 2.7mM KC1) containing 0.05% Tween-20 for 3 times, lOO ⁇ l of antibody solution consisting of 50 mM Tris-HCl (pH 8.0), 138mM NaCl, 2.7mM KC1, 1% BSA, 60ng/ml anti-phosphotyrosine monoclonal antibody, 4G10 (Upstate Biotechnology), which was labeled with europium by Amersham Pharmacia's kit in advance, was added and incubated at room temperature for 60 minutes. After washing, lOO ⁇ l of enhancement solution (Amersham Pharmacia
  • the A549 human lung epithelium cell line (ATCC #CCL-885) was maintained in Dulbecco's modified Eagle's medium (D-MEM, Nikken Biomedical Institute) supplemented with 10% FCS (Gibco), lOOU/ml penicillin, lOO ⁇ g/ml streptomycin, and 2mM glutamine (culture medium).
  • D-MEM Dulbecco's modified Eagle's medium
  • FCS Gibco
  • lOOU/ml penicillin lOO ⁇ g/ml streptomycin
  • 2mM glutamine culture medium
  • Blocking buffer containing 1% BSA (Sigma 99% pure, lOOg), 5% sucrose (Nacalai tesque, 99% pure, 500 g), and 0.02% azide (Nacalai tesque, 100%, 500 g) were added (200 ⁇ l) to each well and then the plate was allowed to stand for 4 hrs. to stabilize the coated antibody.
  • the enhancement solution (DELFIA, #1244-405, lOO ⁇ l/well) was added to each well. The plate was incubated for 10 minutes at room temperature with moderate shaking. Fluorescent intensity was measured using a DELFIA fluorimeter (Wallac). Excitation was performed at 340nm and emission was measured at 615nm.
  • IL-2 production was measured in Jurkat T cells (E6-1 clone; ATCC # TIB-152) in response to stimulation with anti-CD3/anti-CD28 antibodies.
  • anti-CD3 antibodies 400 ng/well Nichirei, NU-T3 4 ⁇ g/ml in lOO ⁇ l Dulbecco's PBS
  • the plate was allowed to stand for 2 hrs. at room temperature to be coated with the antibody.
  • Each well of the plate was then washed with 250 ⁇ l PBS 3 times.
  • Jurkat T cells were cultured in RPMI 1640 medium supplemented with 10% heat-inactivated fetal calf serum, 2 mM L-glutamine, 100 U/ml penicillin G, and lOO ⁇ g/ml streptomycin (culture medium). Two hundred thousand
  • (2xl0 5 ) cells (190 ⁇ l/well) were seeded in each well of 96-well U-bottom tissue culture plates (Falcon #3077). To each well was added lO ⁇ l vehicle (0.2%) DMSO), serial dilution of compounds in 0.2% DMSO, or 25 nM cyclosporin A as a reference in 0.2% DMSO. The mixture (200 ⁇ l) was incubated for 1 hr. at 37°C in a humidified 5% CO environment.
  • reaction mixture obtained in (2) (lOO ⁇ l) was put in the each well of the antibody-immobilized plate prepared in (1).
  • anti- CD28 antibodies Naichirei, KOLT-2, 6 ⁇ g/ml in cell culture medium,
  • the supernatants of the reaction mixture were then collected.
  • the IL-2 concentration in the supernatants was determined using a DuoSetTM ELISA
  • Recombinant Human IL-2 (Genzyme Techne) was used as the standard for the determination of IL-2 production (linear range between 200 and 5,400pg/ml).
  • the reaction mixtures were incubated for 1 hr. at room temperature.
  • lOO ⁇ l biotinylated rabbit anti-huIL-2 antibody (Genzyme Techne, 1.25 ⁇ g/ml) in dilution buffer was added to each well, and incubated at room temperature for 1 hr.
  • lOO ⁇ l of Streptavidin-conjugated horseradishperoxidase (Genzyme Techne, 1/1000 in dilution buffer) was added to each well. After 20 minutes, each well of the plate was washed 5 times with wash buffer (350 ⁇ l/well).
  • Substrate and H 2 O 2 (TMBZ peroxidase detection kit, SUMILON #ML-
  • mice Eight weeks old BALB/c female mice were placed into two groups, a control group and a treated group.
  • a solution containing 200 ⁇ g/mouse of LPS in 0.9%) physiological salt was administered by intraperitoneal (i.p.) injection into the control mice.
  • Mice in the treated group were first injected i.p. with compounds of the present invention 30 minutes prior to the LPS injection.
  • Under anesthesia with pentobarbital (80mg/kg, i.p.) blood was collected from the posterior venous cavity) of the treated and control mice at 90 min post-LPS injection into 96-well plate containing 2% EDTA solution.
  • the plasma was separated by centrifugation at 1800 rpm for 10 minutes at 4°C and then diluted with four times volumes of phosphate buffer saline (pH 7.4) containing 1% bovine serum albumin. TNF- ⁇ concentration in the sample was determined using an ELISA kit (Pharmingen, San Diego, CA.)
  • mice The mean TNF- ⁇ level in 5 mice from each group was determined and the percent reduction in TNF- ⁇ levels was calculated.
  • the treated mice showed significant decrease in the level of TNF- ⁇ as compared to the control mice. The result indicates that the compounds of the present invention can restrain LPS-induced cytokine activity.
  • fr ⁇ vitroIC 5 o A 0.5 ⁇ M ⁇ B 2 ⁇ M ⁇ C 10 ⁇ M ⁇ D
  • Cellular IC 50 A 1 ⁇ M ⁇ B 10 ⁇ M ⁇ C
  • the compounds of the present invention also show excellent selectivity, and strong activity in vivo assays.
  • Coupling constant (J) are given in hertz and the abbreviations s, d, t, q, m, and br refer to singlet, doblet, triplet, quartet, multiplet, and broad, respectively.
  • the mass determinations were carried out by MAT95 (Finnigan MAT).

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Abstract

4-aryl pyrimidine compounds of general formula and salts thereof:wherein X is CH or N; R1 is hydrogen, halogen, C1-6 alkyl, C¿1-6? alkoxy, etc.; R?2¿ is hydrogen, halogen, etc.; R3 is hydrogen, hydroxy, halogen, amino, etc.; R4 is hydroxy, amino, carboxy, etc. or R?3 and R4¿ together form a ring; R5 is hydrogen, carbamoyl, cyano, carboxy, C¿1-16? alkoxycarbonyl, etc. and R?6¿ is amino or C¿1-16? alkanoylamino or R?5 and R6¿ together form a ring. The compounds (I) or the salts thereof have an excellent anti-inflammatory activity and act as inhibitors of IKK-β and thus inhibit nuclear factor kappa B(NF-¿K?B).

Description

4-ARY PYRIDINE DERIVATIVES
DETAILED DESCRIPTION OF INVENTION
TECHNICAL FIELD
The present invention relates to novel 4-aryl pyridine derivatives, processes for preparing them and pharmaceutical preparations containing them. The 4-aryl pyridine derivatives of the present invention inhibit MB kinase β (IKK-β or IKK- beta) activity, thus inhibit nuclear factor kappa B (NF-κB) activity, and can be used for the prophylaxis and treatment of diseases associated with NF-κB activity, in particular for the treatment of inflammatory diseases.
BACKGROUND ART
Nuclear factor kappa B (NF-κB) belongs to a family of closely related homo-and hetero- dimeric transcription factor complexes composed of various combinations of the Rel/NF-κB family of polypeptides. NF-κB and related family members are involved in the regulation of more than 50 genes relating to immune and inflammatory responses ((Barnes PJ, Karin M (1997) N Engl J Med 336, 1066-1071) and (Baeuerle PA, Baichwal VR (1997) Adv Immunol 65, 111-137)). In most cell types, NF-κB is present as a heterodimer comprising a 50 kDa and a 65 kDa subunit (p50/RelA). The heterodimer is sequestered in the cytoplasm in association with inhibitor of NF-κB (IκB)-family of proteins to be kept in an inactive state. IκB- family proteins mask the nuclear translocation signal of NF-κB. Upon stimulation of cells with various cytokines (e.g. TNF-α, IL-1), CD40 ligand, lipopolysaccharide (LPS), oxidants, mitogens (e.g. phorbol ester), viruses or many others. IκB proteins are phosphorylated at specific serine residues, poly-ubiquitinated, and then degraded through a proteasome-dependent pathway. Freed from IκB, the active NF-κB is able to translocate to the nucleus where it binds in a selective manner to preferred gene- specific enhancer sequences. Among the genes being regulated by NF-κB are many coding for pro-inflammatory mediators, cytokines, cell adhesion molecules, and acute phase proteins. Expression of several of these cytokines and mediators in turn can lead to further activation of NF-κB via autocrine and paracrine mechanisms.
Broad evidence is available that suggests a central role of NF-κB in many inflammatory disorders including airway inflammation and asthma ((Yang L et al., J Exp Med 188 (1998), 1739-1750), (Hart LA et al. Am J Respir Crit Care Med 158 (1998), 1585-1592), ( Stacey MA et al., Biochem Biophys Res Commun 236 (1997), 522-526) (Barnes P and Adcock IM, Trends Pharmacol Sci 18 (1997), 46-50)).
Further, it has been shown that glucocorticoids, which are by far the most effective treatment for asthma, inhibit airway inflammation by directly interacting with and inhibiting the activity of the transcription factors NF-κB and activating peptide- 1 (AP-1) ((Barnes P (1997) Pulmon Pharmacol Therapeut 10, 3-19) and (Dumont A et al. (1998) Trends Biochem Sci 23, 233-235)).
In general, inhibition of NF-κB activation results in strong anti-inflammatory effects similar or superior to those brought upon by steroids. Consequently, NF- B inhibition should improve inflammatory symptoms typical for asthma; allergic rhinitis; atopic dermatitis; hives; conjunctivitis; vernal catarrh; rheumatoid arthritis; systemic lupus erythematosus; psoriasis; diabrotic colitis; systemic inflammatory response syndrome; sepsis; polymyositis; dermatomyositis; Polyaritis nodoa; mixed connective tissue disease; Sjoegren's syndrome; gout, and the like.
Further, several studies imply that NF-κB plays an essential role in neoplastic transformation. For example, NF-κB is associated with cell transformation in vitro and in vivo as a result of gene overexpression, amplification, rearrangement, or translocation (Mercurio, F., and Manning, A.M. (1999) Oncogene, 18:6163-6171). In certain human lymphoid tumor cells, the genes of NF-κB family members are rearranged or amplified. Its possible involvement in cancer pathology is also disclosed in Mayo, M.W., Baldwin A.S. (2000) Biochmica et Biophysica Acta 1470 M55-M62. Mayo M.W. et al., discloses the inhibition of NF-κB results in the blockage the initiation and/or progression of certain cancer, particularly colorectal cancer.
Finally, NF-κB may also be involved in the regulation of neuronal cell death. It has been shown that NF-κB becomes activated and promotes cell death in focal cerebral ischemia (Nature medicine Vol. 5 No. 5, May 1999).
Extensive research during the past years led to the identification of an IκB kinase (IKK) complex as being responsible for the signal-induced IκB phosphorylation
(Hatada, E.N, et al. (2000) Current Opinion in Immunology, 12:52-58)). This complex is most likely the site of integration of all of the different stimuli leading to NF-κB activation. The IKK-complex (molecular weight 700 - 900 kDa) is composed of various proteins including two homologous IκB kinases, called IKK-α and IKK- β, an upstream kinase, NIK which induces NF-κB, a scaffold protein called IKAP, which tethers together the three kinases, and a regulatory subunit IKK-γ, which preferentially interacts with IKK-β.
IKK-β is a 756 amino acid serine-threonine kinase showing 52 % identity to and the same domain structure as IKK-α ((Mercurio F et al. (1997) Science 278, 860-866.),
(Woronicz JD et al. (1997) Science 278, 866-869.), ( Zandi E et al. (1997) Cell 91,
243-252.). IKK-β forms homo-dimers and hetero-dimers with IKK-α in vitro and in cells, respectively. IKK-β also interacts with IKK-γ, IKAP, NIK and IκBα.
Recombinant IKK-β phosphorylates IκBα and I Bβ at specific serine residues with equal efficacy (Li I et al. (1998) J Biol Chem 273, 30736-30741). IKK-β shows a higher constitutive kinase activity as compared to IKK-α. This is in agreement with data suggesting that over-expression of IKK-β activates the transcription of a NF-κB- dependent reporter gene with a higher efficacy as compared to IKK-α. IKK-β has been shown to be activated in various cell lines or fresh human cells in response to various stimuli including TNF-α, IL-lβ, LPS, anti-CD3/anti-CD28 co-stimulation, protein kinase C and calcineurin, B-cell receptor/CD40 ligand stimulation, and vanadate. IKK-β is activated in fϊbroblast-like synoviocytes (FLS) isolated from the synovium of patients suffering from rheumatoid arthritis or osteoarthritis (Kempiak SJ et al. (1999) J Immunol 162, 3176-3187.). Furthermore, IKK-β can be activated by the structurally related upstream kinases MEKK-1 and NIK, most likely through phosphorylation of specific serine residues within the T-loop (activation loop) and by certain protein kinase C isoforms (Lallena MJ et al. (1999) Mol Cell Biol 19, 2180- 2188.). A catalytically inactive mutant of IKK-β has been shown to inhibit activation of NF-κB by TNF-α, IL-lβ, LPS, anti-CD3/anti-CD28 stimulation (Woronicz JD et al. (1997) Science 278, 866-869). The same effects are observed when MEKK1 or NIK are overexpressed. Additionally, IKK-β mutations in the activation loop inhibited IL-1 and TNF-α signaling (Delhase M et al. (1999) Science 284, 309-313.). Based on the experimental results described above, there is clear-cut evidence for a pivotal involvement of IKK-β in various pathways leading to NF-κB activation.
In summary, the specific inhibition of IKK-β should result in a strong anti- inflammatory and immuno-modulatory effect in vivo with the potential of improving the underlying causes of asthma and other diseases. In addition, anti-tumor and anti- ischemic effects of an IKK- inhibitor may be expected.
Manna et al., disclose 4,6-disubstituted 3-cyano-2-aminopyridmes represented by general formulas:
wherein
(R', R") represent (OCH3, OCH3), (CI, CI), (H, CI), (H, Br), (H, CH3), (H, OCH3), (H, NO2), or (H, N(CH3)2),
and
as a general anti-inflammatory, analgesic, and antipyretic agent (Eur J. Med. Chem. 34, 245-254(1999)).
However, the development of a novel compound having effective anti-inflammatory actions based on a specific and selective inhibitory activity to NF-κB has been still desired.
SUMMARY OF THE INVENTION
As a result of extensive studies on chemical modification of pyridine derivatives, the present inventors have found that the compounds of novel chemical structure related to the present invention have unexpectedly excellent NF-κB inhibitory activity based on IKK-β inhibition and cytokine inhibition. The present invention has been accomplished based on these findings.
This invention is to provide a novel 4-aryl pyridine derivatives shown by the following formula (I) and salts thereof.
wherein
X is CH orN;
R1 is hydrogen, hydroxy, halogen, -6 alkyl, alkoxy, Ci-β alkoxycarbonyl, nitro, amino, mono or di (d.6 alkyl)amino, phenylsulfonylamino, -NHR11 or -O-(CH2)n-R12 ,
wherein
R ■ π is phenyl substituted Ci-6 alkyl, Cι-6 alkanoyl or Ci-fjalkylsulfonyl,
n represents an integer selected from 0 to 6, and
R 12 is C2.6 alkenyl, benzoyl, mono or di phenyl, mono or di alkyl)amino, alkanoyl, Q-e alkoxycarbonyl, or a 3 to 10 membered saturated or unsaturated ring having 0 to 3 heteroatoms selected from the group consisting of S, O and N, and said ring is optionally substituted by hydroxy, nitro, cyano, mono or di halogen, Cι-6 alkyl, halogen substituted Ci-β alkyl, amino, mono or di (d-6 alkyl)amino, alkoxycarbonyl, or phenyl;
R2 is hydrogen, hydroxy, halogen, or d-6 alkyl; R3 is hydrogen, hydroxy, halogen, d-6 alkoxy, C3.6 cycloalkyl substituted d.6alkyloxy, -N R31R32, or a 3 to 6 membered saturated ring having 0 to 3 heteroatoms selected from the group consisting of O and N, and said ring is optionally substituted by R33;
Wherein
R31 is hydrogen or d-6 alkyl
R32 is hydrogen, Cι-6 alkanoyl, or Cj.6 alkyl optionally substituted by hydroxy or phenyl,
R33 is nitro, cyano, d-6 alkyl optionally substituted by hydroxy or amino, Cι-6 alkoxy, hydroxy substituted -6 alkyloxy, amino substituted d-6 alkyloxy, d-6 alkanoyl, carbamoyl or -NR33aR33b
Wherein
R ,33a is hydrogen or d-6 alkyl
R33b is hydrogen, d-6 alkyl optionally substituted by hydroxy or phenyl, d-6 alkanoyl, d.6 alkylsulfonyl, or trifluoroacetyl,
R4 is hydrogen, hydroxy, carboxy, -CO-NHR41, amino, d-6 alkylsulfonylamino, or -NH-COR41, d_6 alkyl optionally substituted by R42, d-6 alkoxy, R43d-6 alkyloxy,
wherein is d-6 alkyl optionally substituted by R41a, d-6 alkoxy, oxo- tetrahydrofuryl, oxopyrrolidinyl, -CH(OH)R41 , -CH(NH2)R41c, -NHR41c, or piperazino optionally substituted by R41d,
wherein
R41a is carboxy, d.6 alkoxy, -CH(NH2)carboxy, -NR 1a_1R41a"2, or a
3 to 10 membered saturated ring having 0 to 3 heteroatoms selected from the group consisting of O and N, and said ring is optionally substituted by carboxy, d-6 alkyl optionally substituted by hydroxy or benzodioxane, C3.6 cycloalkyl, d-6 alkanoyl, carboxy, benzyl, d.6 alkoxycarbonyl, or furoyl,
wherein
R la_1 is hydrogen or Cι-6 alkyl optionally substituted by hydroxy, Cι_6 alkyloxy, C3.8 cycloalkyl, or piperidino,
R41a"2 is hydrogen or d-6 alkyl optionally substituted by hydroxy, d_6 alkyloxy or C3_6 cycloalkyl, Cι-6 alkoxy or a 3 to 6 membered saturated ring having 0 to 3 heteroatoms selected from the group consisting of O and N, and said ring is optionally substituted by carboxy, d.6 alkyl, d_6 alkanoyl, or d_6 alkyloxy,
R41b is d_6 alkyl optionally substituted by carboxy or d_6 alkyloxy, Ci.<5 alkoxy, or d_6 alkoxycarbonyl,
R41c is carboxy, d-6 alkyl optionally substituted by carboxy or a 3 to 6 membered saturated ring having 0 to 3 heteroatoms selected from the group consisting of O and N, or
R41d is d_6 alkyl optionally substituted by carboxy or d-6 alkyloxy, or d-6 alkoxy
R >42 is d-6 alkoxy, carboxy, amino, -CH(NH2)-carboxy or a 5 to 7 membered saturated or unsaturated ring having 0 to 3 heteroatoms selected from the group consisting of O and N, and said ring is optionally substituted by hydroxy, nitro, mono or di halogen, d-6 alkyl, halogen substituted d-6 alkyl, amino, mono or di (d-6 alkyl)amino, or carbamoyl;
R43 is carboxy, amino, -CH(NH2)-carboxy or a 5 to 7 membered saturated or unsaturated ring having 0 to 3 heteroatoms selected from the group consisting of O and N, and said ring is optionally substituted by hydroxy, nitro, mono or di halogen, d-6 alkyl, halogen substituted d_6 alkyl, amino, mono or di (d.6 alkyl)amino, or carbamoyl;
or
R3 and R4 may form, together with the carbon atoms in the benzene ring, a 4 to 6 membered saturated ring having 0 to 3 heteroatoms selected from the group consisting of O and N, and said ring is optionally substituted by one or more substituents selected from the group consisting of hydroxy, nitro, mono or di halogen, d_6 alkyl, halogen substituted d-6 alkyl, oxo, amino, mono or di (Cι-6 alkyl)amino, and carbamoyl;
R5 is hydrogen, cyano, carboxy, carbamoyl, d-6 alkyl optionally substituted by hydroxy or carbamoyl, and d-6 alkoxycarbonyl, and R° is -NR61R62.
wherein
R61 is hydrogen or d-6 alkyl,
R62 is hydrogen, d_6 alkyl, phenyl, benzyl, d.6 alkanoyl, or
NR61 R62 may form, together with the nitrogen atom to which they are attached, a saturated 5-6 membered ring optionally containing NH or
O as other heteroatoms than the adjacent N atom
or
R5 and R6 may form, together with the carbon atoms in the pyridine ring, a 5 to 7 membered saturated or unsaturated ring having 0 to 3 heteroatoms selected from the group consisting of O, S and N, and said ring optionally having substituents selected from the group consisting of halogen, nitro, cyano, oxo, thioxo, d-6 alkyl, d-6 alkylsulfonyl, d_6 alkoxy, d-6 alkoxycarbonyl, phenyl, d-6 alkanoyl, amino, d-6 alkylamino, d_6 alkanoylamino, carbamoyl, C3.8 cycloalkylaminocarbonyl, d-6 alkylaminocarbonyl, halogen substituted d_6 alkylaminocarbonyl, di (d_6 alkyl)aminocarbonyl, benzoyl- amino, phenylsulfonyl, di(d-6 alky aminod-e alkylaminocarbonyl, hydroindenylaminocarbonyl, diphenylmethylaminocarbonyl, pyrrolidino- carbonyl, d-6 alkyloxy d-6 alkyl amino carbonyl, morpholinocarbonyl, piperazinocarbonyl, phenyld .6alkylaminocarbonyl, carboxyC ι _6alkylamino- carbonyl, C3.8 cycloalkyld-6alkylaminocarbonyl, hydroxyd-6alkylamino- carbonyl, and methylsulfonylaminocarbonyl
or a salt thereof. The compounds of the present invention surprisingly show excellent IKK-β kinase inhibitory activity and cytokine inhibitory activity. They are, therefore suitable especially as NF- B inhibitors and in particular for the production of pharmaceuticals or pharmaceutical compositions, which may be useful to treat NF-κB dependent diseases.
More specifically, since the 4-aryl pyridine derivatives of the present invention inhibit IKK-β kinase activity, they are useful for treatment and prophylaxis of diseases involving NF-κB activity as follows: inflammatory symptoms including asthma; allergic rhinitis; atopic dermatitis; hives; conjunctivitis; vernal catarrh; chronic arthrorheumatism; systemic lupus erythematosus; psoriasis; diabrotic colitis; systemic inflammatory response syndrome (SIRS); sepsis; polymyositis; dermatomyositis (DM); polyarthritis nodosa (PN); mixed connective tissue disease
(MCTD); Sjoegren's syndrome; gout; and the like.
The compounds of the present invention are also useful for treatment and prophylaxis of diseases like ischemia and tumor, since the diseases also relate to IKK-β kinase and NF-κB activity.
Preferred compounds of the formula (I) are those wherein:
X is CH orN;
R1 is hydrogen, halogen, C1.6 alkoxy, or cyclopropyl methoxy;
R2 is hydrogen;
R3 is hydrogen, hydroxy, halogen, amino, mono or di(Cι-6 alkyl)amino, acetamido, d-6alkoxy, cyclopropyl methoxy, d_6 alkyl(hydroxy substituted Cι-6 alkyl)amino, d-6 alkyl(benzyl)amino, morpholino, piperidino, or pyrrolidino optionally substituted by d-6 alkyl, hydroxy substituted d_6 alkyl, amino, di (d-6 alkyl)amino, or trifluoroacetylamino;
R4 is hydrogen, hydroxy, carboxy, amino, d-6 alkylsulfonylamino, piperazino, d-6 alkyl substituted piperazino, piperidino substituted Cj.6 alkyloxy, piperidino d-6 alkyl- aminocarbonyl, or-NH-COR41,
wherein
R41 is d-6 alkyl optionally substituted by R41a, d_6 alkoxy, oxotetrahydrofuryl, oxopyirolidinyl, -CH(OH)R41 , or -CH(NH2)R41c,
wherein
R41a is Cι-6 alkoxy, carboxy, -CH(NH2)-carboxy, -NHR413"1
(wherein R41a_1 is hydrogen, d-6 alkyl optionally substituted by hydroxy, C3.8 cycloalkyl, d.6 alkoxy, or piperidino), -N(d.6 alkyl)R41a'2 (wherein R41a"2 is d-6 alkyl or d-6 alkyl substituted pyrrolidinyl), pyrrolidinyl, piperidino optionally substituted by carboxy or d_6 alkoxycarbonyl, piperidino fused with cyclohexane, or piperazino optionally substituted by d-6 alkyl, hydroxy substituted d-6 alkyl, benzodioxane substituted d_6 alkyl, benzyl, Cι-6 alkanoyl, cyclohexyl, or furoyl,
R41 is Cι-6 alkyl optionally substituted by carboxy or alkoxycarbonyl,
R41c is d-6 alkyl optionally substituted by carboxy, or
R )3J „ an„d r>4÷ ^ ma^yr Λ fo,_rm - -NNτRπ440υ1ι-C ,-.O- ΓO-( Λw,Λh,e___,r-_e_,in Γ R>W°11 is hydrogen or Cj.6 alkyl); R5 is hydrogen, carbamoyl, cyano, carboxy, or hydroxymethyl; and
R »6 is amino or acetamido
or
R5 and R° may form -R50-CH2-NH-, -R50-CO-NH-, -R50-SO2-NH-, or -R
C(=S)- H-
wherein
R50 is -CHR501-O-, -CH2-N R501-, -CO-NR501-, (wherein R501 is hydrogen, d-6 alkyl, d-6 alkanoyl, d-6 alkoxycarbonyl, carbamoyl, Cι-6 alkylsulfonyl, C3.8 cycloalkylaminocarbonyl, d_6 alkylaminocarbonyl, di (CΪ-6 alkylaminocarbonyl or phenyl)
or a salt thereof.
In the context of the present invention, the substituents, if not stated otherwise, in general have the following meaning:
d-6 alkyl in general represents a straight-chain or branched hydrocarbon radical having 1 to 6 carbon atoms. Non-limiting examples include methyl, ethyl, propyl, isopropyl, butyl, isobutyl, pentyl, isopentyl, hexyl, isohexyl.
Cι-6 alkoxy in general represents a straight-chain or branched hydrocarbon radical having 1 to 6 carbon atoms and bound via an oxygen atom. Non-limiting examples include methoxy, ethoxy, propoxy, isopropoxy, butoxy, isobutoxy, pentoxy, isopentoxy, hexoxy, isohexoxy. C3-s cycloalkyl in general represents a cyclic hydrocarbon radical having 3 to 8 carbon atoms. Cyclopropyl, cyclopentyl and cyclohexyl are preferred. Non-limiting examples include cyclopentyl, cyclohexyl, cycloheptyl and cyclooctyl.
C2_6 alkenyl stands for straight-chain or branched residues containing one or more double bonds, e.g. vinyl, allyl.
The preferable compounds of the present invention are as follows:
5-({3-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl}amino)-4- hydroxy-5-oxopentanoate;
N-{3-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl}-5-oxotetrahydro-
2-furancarboxamide;
N-{3-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl}methane- sulfonamide; N4- {3-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl} -L-asparagine;
N1-{3-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl}-L-asparagine;
N-{3-[2-ammo-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl}-3-(l-piperidinyl)- propanamide;
5-({4-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl}amino)-4- hydroxy-5-oxopentanoate;
4-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-N-[2-(l-piperidinyl)ethyl]- benzamide;
4-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]benzoic acid;
5-({5-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-2-chlorophenyl}amino)- 4-hydroxy-5-oxopentanoate;
N-{3-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl}-2-[4-(2-furoyl)-l- piperazinyljacetamide;
N-{3-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl}-3-(4-ethyl-l- piperazinyl)propanamide; N-{3-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl}-3-octahydro-
1 (2H)-quinolinylpropanamide; N1 - {3 -[2-amino-3 -cyano-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl} -N2- cyclopentylglycinamide;
N1-{3-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl}-N2-(cyclopropyl- methyl)glycinamide; N1- {3-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl} -N2-propyl- glycinamide;
N1-{3-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl}-N2-(3-hydroxy- propyl)glycinamide;
N1-{3-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl}-N3-(cycloρropyl- methyl)-β-alaninamide;
N1-{3-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl}-N2,N2-dimethyl- glycinamide;
N- {3-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl} -2-(l - pyrrolidinyl)acetamide; N1-{3-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl}-N2-[2-
(methyloxy)ethyl] glycinamide;
N1 - [5 -[2-amino-3 -cy ano-6-(2-hy droxyphenyl)-4-pyridinyl]-2-(3 -amino- 1 - pyrrolidinyl)phenyl]-β-alaninamide;
N1-[5-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-2-(l-piperidinyl)phenyl]- β-alaninamide;
N1-[5-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-2-(l-pyrrolidinyl)- phenyl]-β-alaninamide;
N1-{5-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-2-[ethyl(2-hydroxy- ethyl)amino]phenyl}-β-alaninamide; N1-[5-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-2-(4-morpholinyl)- phenyl]-β-alaninamide;
N1-[5-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-2-(dimethylamino)- phenyl]-β-alaninamide;
N-{3-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-4-chlorophenyl}-3-(l- piperidinyl)propanamide;
2-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]benzoic acid; 2-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-N-[2-(l-piperidinyl)ethyl]- benzamide;
1 9
N -{5-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyπdmyl]-2-chlorophenyl}-N -
(cyclopropylmethyl)glycinamide; N1-{5-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-2-chlorophenyl}-N2-(2- methoxyethyl)glycinamide;
N1 - { 5- [2-amino-3 -cyano-6-(2-hydroxyphenyl)-4-pyridinyl] -2-chlorophenyl } -β- alaninamide;
2-amino-4-(4-amino-3-hydroxyphenyl)-6-(2-hydroxyphenyl)nicotmonitrile; N- {4-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-2-hydroxyphenyl} - acetamide;
N- { 5 - [2-amino-3 -cyano-6-(2-hydroxyphenyl)-4-pyridinyl] -2-chlorophenyl} -3 -( 1 - piperidinyl)propanamide;
N1-[5-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-2-(dimethylamino)- phenyl] -N2-(cyclopropylmethyl)glycinamide;
N-[5-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-2-(dimethyl- amino)phenyl] -3 -( 1 -piperidinyl)propanamide;
N1-{5-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-2-[(2S)-2-
(hydroxymethyl)- 1 -pyrrolidinyl]phenyl} -L-leucinamide; N1-{5-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-2-[(3S)-3-
(dimethylamino)- 1 -pyrrolidinyljphenyl} -N -(cyclopropylmethyl)glycmamide;
N1-(5-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-2-{3-[(trifluoroacetyl)- amino]- 1 -pyrrolidinyl}phenyl)-L-leucinamide;
N-(5-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-2-{3-[(trifluoroacetyl)- amino]-l-pyrrolidinyl}phenyl)-3-(l-piperidinyl)propanamide;
N1-(5-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-2-{3-[(trifluoroacetyl)- amino]-l-pyrrolidinyl}phenyl)-N2-(2-methoxyethyl)glycinamide;
N1-(5-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-2-{3-[(trifluoro- acetyl)amino]-l-pyrrolidinyl}phenyl)-N2-(cyclopropylmethyl)glycinamide; 2-amino-6-(2-hydroxyphenyl)-4-(3-hydroxyphenyl)nicotinonitrile; N-{4-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-2-[2-(l-piperidinyl)- ethoxy]phenyl} acetamide;
N-{4-[2-ammo-3-(hydroxymethyl)-6-(2-hydroxyphenyl)-4-pyridinyl]-2-hydroxy- phenyl} acetamide; 2-[6-amino-4-[4-(emylamino)-3-hy(_hoxyphenyl]-5-(hyd oxymethyl)-2-pyridinyl]-3-
(cyclopropyhnethoxy)phenol;
N-{4-[2-amino-6-[2-(cyclopropylmethoxy)-6-hydroxyphenyl]-3-(hydroxymethyl)-4- pyridinyl]-2-hydroxyphenyl}acetamide;
7-[2-(cyclopropylmethoxy)-6-hydroxyphenyl]-5-(3-ethyl-2-oxo-2,3-dihydro-l,3- benzoxazol-6-yl)-l ,4-dihydro-2H-pyrido[2,3-d] [1 ,3]oxazin-2-one;
and pharmaceutically acceptable salts thereof.
The compound of the formula (I) of the present invention can be, but not limited to be, prepared by combining various known methods. In some embodiments, one or more of the substituents, such as amino group, carboxy group, and hydroxy group of the compounds used as starting materials or intermediates are advantageously protected by a protecting group known to a person skilled in the art. Examples of the protecting groups are described in "Protective Groups in Organic Synthesis (2nd Edition)" by Greene and Wuts.
The typical processes for preparing the compounds (I) of the present invention are explained below.
(1) The compound of the formula (I-a):
in which
X, R ,ι , R and R are the same as defined above and R 4a i •s d-6 alkylsulfonyl, or -COR41 (R41 is the same as defined.),
can be prepared, for example, by the following reaction A.
Reaction A
In the step 1, the compound of the formula (II):
(wherein
R1, R2 and X are the same as defined above), is reacted with an aldehyde of the formula (III):
(wherein
R3 is the same as defined above), malononitrile, and ammonium acetate to prepare the compound (IV).
In step 2, the nitro group is reduced to convert to the amino group.
In step 3, the amino group is further modified to obtain a desired group (NHR 4a ).
The substituents of the compound (II) or (III) are, if necessary, protected by an appropriate protecting group (e.g., benzyl, silyl) during the reaction, and deprotected afterward.
The step 1 is carried out without a solvent or in a solvent including, for instance, ethers, such as dioxane, and tetrahydrofuran; aromatic hydrocarbons such as benzene, toluene and xylene; nitriles such as acetonitrile; amides such as dimethylformamide (DMF) and dimethylacetamide; sulfoxides such as dimethyl sulfoxide, and others.
The reaction temperature is optionally set depending on the compounds to be reacted. The reaction temperature is usually about 50°C to 200°C. The reaction is conducted for, usually, 30 minutes to 48 hrs. and preferably 1 to 24 hrs. The compounds of (II) and (III) can be commercially available, or can be prepared by the use of known techniques.
The step 2 is carried out in the presence of a reducing agent and a hydrogen donor at about room temperature to 120°C. The reaction time and the solvent used in the step 2 are similar as that used in the step 1.
The step 3 is carried out under the condition usually employed by a skilled person in the art to synthesize amides (NHR4a).
(2) Alternatively, the compound of the formula (I-b):
wherein
X, R1, R2, R3 and R41 are the same as defined above, can be prepared, for example, by the following reaction B.
Reaction B
The compound (IΙ)is reacted with an aldehyde of the formula (III'):
(wherein
R is the same as defined above, and R 4 is amino or alkoxy), malononitrile, and ammonium acetate to prepare the compound (IV). R 4b is further modified to obtain a desired group (OH or NHR41).
The substituents of the compound of (II) or (III) are, if necessary, protected by an appropriate protecting group (e.g., benzyl, silyl) during the reaction, and deprotected afterward.
The step 1 of the reaction B can be carried out under the similar condition to that of reaction A. The step 2 in the reaction B can be hydrolysis and can be carried out under the condition usually employed by a skilled person in the art.
The step 3 in the reaction B can be carried out under the condition usually employed by a skilled person in the art to synthesize amides (CONHR41).
(3) Alternatively, the compound of the formula (I-c):
wherein
X, R1, R2, R3 and R4 are the same as defined, and R5a is carboxy, carbamoyl, d-6 alkoxycarbonyl, hydroxy Cι-6 alkyl, d_6 alkylcarbamoyl, or Cι.6 alkyl,
can be prepared, for example, by the following reaction C.
OOtBu + CN + AcONH4
Reaction C
The compound (II) is reacted with an aldehyde of the formula (111"):
(wherein
R3 and R4 are the same defined above),
a nitrile of the formula, tBu-OCO-CH CN, and ammonium acetate to prepare the compound (IN"). Then COOtBu of the resulting compound (IN") can be modified to obtain a desired compound (R5a).
The substituents of the compound of (II) or (III") may be protected by an appropriate protecting group (e.g., benzyl, silyl) during the reaction, and deprotected afterward.
The step 1 of the reaction C can be carried out under the similar condition to that of reaction A. The step 2 of the reaction C can be, for example, a hydrolysis reaction, decarboxylation, reducing reaction, amide synthesis, etc. The conditions of the step 2 can be the same as that usually employed by a skilled person in the art.
The amino group at position 2 of the pyridine ring in the compounds (I-a), (I-b) and (I-c) is, if necessary, modified according to conventional method to prepare other groups such as alkylamino, alcanoylamino, and the like.
Typical procedures for preparing saturated or unsaturated heteroring compounds that are formed by R5 and R6 in formula I, together with the carbon atoms in the pyridine ring, are as follows.
The starting compound is I-d: wherein X, R1, R2, R3 and R4 are the same as defined above.
In the step 1 of reaction D, starting compound of formula I-d in a suitable solvent with suitable organic base can be treated with triphosgene by known method to give a desired compound represented by the formula I-D.
In the step 1 of reaction E, starting compound of formula I-d in a suitable solvent can be treated with oxidizing agent such as manganese dioxide, nitric acid and pyridinium chlorochromate (PCC) for preparing a compound represented by the formula I-d'-l.
In the step 2 of reaction E, a formula I-d'-l in a suitable solvent can be treated with diethylmalonate and suitable base to give a desired compound represented by the formula I-E.
In the step 1 of reaction F, starting compound of formula I-d in a suitable solvent can be treated with phosphonic ester reagent such as diphenylphosphoryl azide (DPP A) and suitable base for preparing a compound represented by the formula I-d'-2.
In the step 2 of reaction F, compound I-d'-2 can be treated with hydrogen in the presence of a metal catalyst such as palladium on carbon (Pd/C) in a solvent for preparing a compound represented by the formula I-d'-3.
In the step 3 of reaction F, compound I-d' -3 in a suitable solvent can be treated with l,l'-carbonyldiimidazole (CDI) to give a desired compound formula I-F-l.
In the step 4 of reaction F, compound I-d'-3 can be treated with sulfamide and suitable base to give a desired compound represented by the formula I-F-2.
The suitable solvents for the individual steps in the reactions D to F are not limited, but can be selected from the group consisting of ethers, such as dioxane, diethylether, tetrahydrofuran (THF), glycol dimethyl ether, or alcohols such as, propanol, butanol, isopropanol, ethanol diisopropyl ether or esters, such as ethyl acetate, butyl acetate 1, or aromatic hydrocarbons such as benzene, toluene, xylene, hexane or nitriles such as acetonitrile or amides such as dimethylformamide (DMF), dimethylacetamide or sulfoxides such as dimethyl sulfoxide or halogenated hydrocarbons such as dichloromethane, trichloromethane and others. Having described some examples of suitable solvents, the present examples are illustrative and not restrictive. The suitable solvents for the individual steps can be selected by the person skilled in the art.
The suitable inorganic or organic bases for the individual steps in the reactions D to F are not limited, but can be selected from the group comprising for instance,such as alkaline earth metal or alkali metal hydroxides, alkoxides, acetates, carbonates or bicarbonates, such as, sodium hydroxide, potassium hydroxide or ammonium hydroxide, sodium methoxide, sodium ethoxide, or potassium tert-butoxide, sodium acetate, potassium acetate, calcium acetate or ammonium acetate, sodium carbonate, potassium carbonate or ammonium carbonate, sodium bicarbonate or potassium bicarbonate and also tertiary amines, such as trimethylamine, triethylamine, tributyl- amine, ethyl-diisopropylamine, N,N-dimethylaniline, N,N-dimethyl-benzylamine, pyridine, picoline, N-methylpiperidine, N-methylmorpholine, N,N-dimethylamino- pyridine, diazabicyclooctane (DABCO), diazabicyclononene (DBN) or diazabicyclo- undecene (DBU). Having described some examples of suitable inorganic and organic bases, the present examples are illustrative and not restrictive. The suitable bases for the individual steps can be selected by the person skilled in the art.
The reaction temperature is optionally set depending on the compounds to be reacted. The reaction temperature is usually about 0°C to 150°C. The reaction is conducted for, usually, 30 minutes to 48 hrs. and preferably 1 to 24 hrs.
When the compound shown by the formula (I) or a salt thereof has geometrical isomers and/or conformational isomers, each of their separated isomer and mixtures are also included in the scope of the present invention. When the compound shown by the formula (I) or a salt thereof has an asymmetric carbon(s) in the structure, their optically active compounds and racemic mixtures are also included in the scope of the present invention.
Typical salts of the compound shown by the formula (I) include salts prepared by the reaction of the compound of the present invention with a mineral or organic acid, or an organic or inorganic base. Such salts are known as acid addition and base addition salts, respectively.
Acids to form acid addition salts include inorganic acids such as, without limitation, sulfuric acid, phosphoric acid, hydrochloric acid, hydrobromic acid, hydroiodic acid and the like, and organic acids, such as, without limitation, p-toluenesulfonic acid, methanesulfonic acid, oxalic acid, p-bromophenylsulfonic acid, carbonic acid, succinic acid, citric acid, benzoic acid, acetic acid, and the like.
Base addition salts include those derived from inorganic bases, such as, without limitation, ammonium hydroxide, alkaline metal hydroxide, alkaline earth metal hydroxides, carbonates, bicarbonates, and the like, and organic bases, such as, without limitation, ethanolamine, triethylamine, tri(hydroxymethyl)aminomethane, and the like. Examples of inorganic bases include, sodium hydroxide, potassium hydroxide, potassium carbonate, sodium carbonate, sodium bicarbonate, potassium bicarbonate, calcium hydroxide, calcium carbonate, and the like.
Examples of such salts are sulfate, pyrosulfate, bisulfate, sulfite, bisulfite, phosphate, monohydrogenphosphate, dihydrogenphosphate, metaphosphate, pyrophosphate, chloride, bromide, iodide, acetate, propionate, decanoate, caprylate, acrylate, formate, isobutyrate, caproate, heptanoate, propiolate, oxalate, malonate, succinate, suberate, sevacate, fumarate, maleate, butyne-l,4-dioate, hexyne-l,6-dioate, benzoate, chlorobenzoate, methoxybenzoate, phthalate, sulfonate, xylenesulfonate, phenylacetate, phenylpropionate, phenylbutyrate, citrate, lactate, γ-hydroxybutyrate, glycollate, tartarate, methanesulfonate, propanesulfonate, naphthalene- 1 -sulfonate, naphthalene-2-sulfonate, mandelate, and the like salts of the compound of formula (I).
The preferred acid addition salts are those formed with mineral acids, such as, without limitation, hydrochloric acid, and hydrobromic acid, and those formed with organic acids, such as without limitation, maleic acid and methanesulfonic acid. The potassium and sodium salt forms are particularly preferred base addition salts.
The compound of the present invention may be administered in oral forms, such as, without limitation normal and enteric coated tablets, capsules, pills, powders, granules, elixirs, tinctures, solution, suspensions, syrups, solid or liquid aerosols and emulsions. They may also be administered in parenteral forms, such as, without limitation, intravenous, intraperitoneal, subcutaneous, intramuscular, and the like forms, well-known to an ordinary skilled person in the pharmaceutical arts.
The compounds of the present invention can be administered in intranasal form via topical use of suitable intranasal vehicles, or from transdermal routes, using transdermal delivery systems well-known to an ordinary skilled person in the art.
The dosage regimen with the use of the compounds of the present invention is selected by one of ordinary skill in the arts, in view of a variety of factors, including, without limitation, age, weight, sex, and medical condition of the recipient, the severity of the condition to be treated, the route of administration, the level of metabolic and excretory function of the recipient, the dosage form employed, the particular compound and salt thereof employed.
The compounds of the present invention are preferably formulated prior to administration together with one or more pharmaceutically acceptable excipients. Excipients are inert substances such as, without limitation carriers, diluents, flavoring agents, sweeteners, lubricants, solubilizers, suspending agents, binders, tablet disintegrating agents and encapsulating material. Another embodiment of the present invention is a pharmaceutical formulation comprising the compound of the invention and one or more pharmaceutically acceptable excipients that are compatible with the other ingredients of the formulation and not deleterious to the recipient thereof. Pharmaceutical formulations of the present invention are prepared by combining a therapeutically effective amount of the compound of the invention together with one or more pharmaceutically acceptable excipients therefor. In making the compositions of the present invention, the active ingredient may be mixed with a diluent, or enclosed within a carrier, which may be in the form of a capsule, sachet, paper, or other container. The carrier may serve as a diluent, which may be solid, semi-solid, or liquid material which acts as a vehicle, or can be in the form of tablets, pills, powders, lozenges, elixirs, suspensions, emulsions, solutions, syrups, aerosols, ointments, containing, for example, up to 10% by weight of the active compound, soft and hard gelatin capsules, suppositories, sterile injectable solutions and sterile packaged powders.
For oral administration, the active ingredient may be combined with non-toxic, pharmaceutically acceptable carriers, such as, without limitation, lactose, starch, sucrose, glucose, sodium carbonate, mannitol, sorbitol, calcium carbonate, calcium phosphate, calcium sulfate, methyl cellulose, and the like; together with, optionally, disintegrating agents, such as, without limitation, maize, starch, methyl cellulose, agar bentonite, xanthan gum, alginic acid, and the like; and optionally, binding agents, for example, without limitation, gelatin, acacia, natural sugars, beta-lactose, corn sweeteners, natural and synthetic gums, tragacanth, sodium alginate, carboxy- methyl cellulose, polyethylene glycol, waxes, and the like; and, optionally, lubricants, for example, without limitation, magnesium stearate, sodium stearate, stearic acid, sodium oleate, sodium benzoate, sodium acetate, sodium chloride, talc, and the like. In powder forms, the carrier may be a finely divided solid which is in admixture with the finely divided active ingredient. The active ingredient may be mixed with a carrier having binding properties in suitable proportions and compacted in the shape and size desired to produce tablets. The powders and tablets preferably contain from about 1 to about 99 weight percent of the active ingredient which is the novel composition of the present invention. Suitable solid carriers are magnesium carboxymethyl cellulose, low melting waxes, and cocoa butter.
Sterile liquid formulations include suspensions, emulsions, syrups and elixirs. The active ingredient can be dissolved or suspended in a pharmaceutically acceptable carrier, such as sterile water, sterile organic solvent, or a mixture of both sterile water and a sterile organic solvent.
The active ingredient can also be dissolved in a suitable organic solvent, for example, aqueous propylene glycol. Other compositions can be made by dispersing the finely divided active ingredient in aqueous starch or sodium carboxymethyl cellulose solution or in a suitable oil.
The formulation may be in unit dosage form, which is a physically discrete unit containing a unit dose, suitable for administration in human or other mammals. A unit dosage form can be a capsule or tablets, or a number of capsules or tablets. A "unit dose" is a predetermined quantity of the active compound of the present invention, calculated to produce the desired therapeutic effect, in association with one or more excipients. The quantity of active ingredient in a unit dose may be varied or adjusted from about 0.1 to about 1000 milligrams or more according to the particular treatment involved.
Typical oral dosages of the present invention, when used for the indicated effects, will range from about O.Olmg kg/day to about lOOmg/kg/day, preferably from 0.1 mg/kg/day to 30 mg/kg/day, and most preferably from about 0.5 mg/kg/day to about 10 mg/kg/day. In case of parenteral administration, it has generally proven advantageous to administer quantities of about 0.001 to 100 mg/kg/day, preferably from 0.01 mg kg/day to lmg/kg/day. The compounds of the present invention may be administered in a single daily dose, or the total daily dose may be administered in divided doses, two, three, or more times per day. Where delivery is via transdermal forms, of course, administration is continuous.
The effects of the compounds of the present invention were examined by the following assays and pharmacological tests.
IKK-β kinase inhibitory assay.
( 1 ) Preparation of IKK-β kinase protein.
A cDNA fragment encoding human IKK-β open reading frame was generated by PCR with the use of a pair of primers designed from the published sequence (Woronicz JD et al. (1997) Science 278,866-869). A template was obtained from Quickclone cDNA (Clontech) using Elongase™ Amplification kit (Life Technologies). The DNA fragments generated by PCR were gel- purified and subcloned into pBluescript. The cDNA fragment cloned in pBluescript was inserted into pcDNA3.1/His C Kpnl/Notl, and transferred into pNL1393 Smal/Xbal (Pharmingen) to construct a baculovirus transfer vector. Then the vector, together with the linearized baculovirus (BaculoGold™, Pharmingen) was used to transfect Sf21 cells (Invifrogen, San Diego, CA). Generated recombinant baculovirus was cloned and amplified in Sf21 cells, grown in TΝM-FH insect cell medium (Life
Technologies, Inc.) supplemented with 10% FCS, 50 g/ml Gentamycin, 0.1% Pluronic F-68 (Life Technologies, Inc.) as suspension culture (200 ml in 1 L Erlenmeyer flask; 27°C; 130 rpm). Sf21 cells were infected with this amplified virus with a multiplicity of infection of 5 following standard protocols (Crossen R, Gruenwald S (1997) Baculovirus Expression Vector
System Instruction Manual, Pharmingen Corporation) and harvested 48 hrs. later. The cells were lysed to obtain the produced chimeric protein of IKK-β kinase fused by histidine (His-tagged D K-beta).
(2) The preparation of purified GST-IκBα fusion proteins
An expression vector containing the nucleotide sequence encoding fusion protein of GST with amino acid residues 1 to 54 of IKBOC under the control of an IPTG-inducible promoter was constructed. The expression vector was introduced in E. coli and the transformant was cultured and lysed to obtain a GST-IκBα fusion protein. Then the resulting GST-IκBα fusion protein was purified and biotinated for kinase assay.
(3) The measurement of IKK-β kinase activity
The 96-well format kinase assay of IKK-β was performed to test the inhibitory activity of the compounds of the present invention. First, 5μl of a test compound was put in the presence of 2.5% dimethyl sulfoxide (DMSO) in each well in a U-bottomed 96-well plate (Falcon). For control wells of background (BG) and total phosphorylation (TP), 5 μl of 2.5% DMSO was put. Recombinant IKK-β (final 0.6 μg/ml) and bio-GST-IκBα (1-54) (final 0.2 μM) were diluted in 25 μl of 2 x kinase buffer β (40mM Tris-HCl, pH 7.6,
40 mM MgCl2, 40mM β-glycerophosphate, 40mM p-nitrophenyl-phosphate, 2mM EDTA, 40mM creatine phosphate, 2mM DTT, 2 mM Na3VO4, 0.2 mg/ml BSA and 0.8mM phenylmethylsulfonyl fluoride) and transferred to the 96-well plate. Bio-GST-IκBα (1-54) in 25 μl of 2 x kinase buffer β without IKK-β was transferred to BG wells. Then 20μl of 12.5μM ATP,
62.5 μCi/ml [γ-33P] ATP (Amersham Pharmacia Biotech)was added and the resulting mixture was incubated for 2 hrs. at room temperature. The kinase reactions were terminated by the addition of 150 μl of termination buffer (100 mM EDTA, 1 mg/ml BSA, 0.2mg NaN3). One handred and fifty μl of the sample was transferred to a streptavidin-coated, white MTP (Steffens
Biotechniche Analysen GmbH #08114E14.FWD) to capture the biotinylated substrates. After 1 hr. of incubation, non-bound radioactivity was eliminated by washing the wells five times with 300μl of washing buffer including 0.9% NaCl and 0.1 % (w/v) Tween-20 with the use of a MW-96 plate washer (BioTec). The bound radioactivity was determined after the addition of 170μl MicroScint-PS scintillation cocktail (Packard) using a TopCount scintillation counter.
Syk tyrosine kinase inhibitory assay for selectivity.
(1) Preparation of Syk protein
A cDNA fragment encoding human Syk openreading frame was cloned from total RNA of human Burkitt's lymphoma B cell lines, Raji (American Type Culture Collection), with the use of RT-PCR method. The cDNA fragment was inserted into pAcG2T (Pharmingen, San Diego, CA) to construct a baculovirus transfer vector. Then the vector, together with the linearized baculovirus (BaculoGold™, Pharmingen), was used to transfect Sf21 cells (Invifrogen, San Diego, CA).
Generated recombinant baculovirus was cloned and amplified in Sf21 cells.
Sf21 cells were infected with this amplified high titer virus to produce a chimeric protein of Syk kinase fused by glutathione-S-transferase (GST).
The resulting GST-Syk was purified with the use of glutathione column (Amersham Pharmacia Biotech AB, Uppsala, Sweden) according to the manufacturer's instruction. The purity of the protein was confirmed to be more than 90% by SDS-PAGE. (2) Synthesize of a peptide
Next, a peptide fragment of 30 residues including two tyrosine residues, KISDFGLSKALRADENYYKAQTHGKWPVKW, was synthesized by a peptide synthesizer. The N-terminus of the fragment was then biotinylated to obtain biotinylated activation loop peptide (AL).
(3) The measurement of Syk tyrosine kinase activity
All reagents were diluted with the Syk kinase assay buffer (50mM Tris-HCl
(pH 8.0), lOmM MgCl2, O.lmM Na3VO4, 0.1% BSA, 1 mM DTT). First, a mixture (35 μl) including 3.2μg of GST-Syk and 0.5μg of AL was put in each well in 96-well plates. Then 5μl of a test compound in the presence of 2.5% dimethyl sulfoxide (DMSO) was added to each well. To this mixture was added 300μM ATP (10 μl) to initiate the kinase reaction. The final reaction mixture (50 μl) consists of 0.65 nM GST-Syk, 3μM AL, 30μM ATP, a test compound, 0.25% DMSO, and a Syk kinase assay buffer.
The mixture was incubated for 1 hr. at room temperature, and the reaction was terminated by the addition of 120μl of termination buffer (50 mM Tris-
HCl (pH 8.0), lOmM EDTA, 500mM NaCl, 0.1% BSA). The mixture was transferred to streptavidin-coated plates and incubated for 30 minutes, at room temperature to combine biotin-AL to the plates. After washing the plates with Tris-buffered saline (TBS) (50mM Tris-HCl (pH 8.0), 138mM NaCl, 2.7mM KC1) containing 0.05% Tween-20 for 3 times, lOOμl of antibody solution consisting of 50 mM Tris-HCl (pH 8.0), 138mM NaCl, 2.7mM KC1, 1% BSA, 60ng/ml anti-phosphotyrosine monoclonal antibody, 4G10 (Upstate Biotechnology), which was labeled with europium by Amersham Pharmacia's kit in advance, was added and incubated at room temperature for 60 minutes. After washing, lOOμl of enhancement solution (Amersham Pharmacia
Biotech) was added and then time-resolved fluorescence was measured by multi-label counter ARVO (Wallac Oy, Finland) at 340nm for excitation and 615nm for emission with 400 msec of delay and 400 msec of window.
The measurement of RANTES production in response to TNF-α from A549 cells.
( 1 ) Preparation of A549 cells
The A549 human lung epithelium cell line (ATCC #CCL-885) was maintained in Dulbecco's modified Eagle's medium (D-MEM, Nikken Biomedical Institute) supplemented with 10% FCS (Gibco), lOOU/ml penicillin, lOOμg/ml streptomycin, and 2mM glutamine (culture medium).
Forty thousand (4 x 104) cells (80μl/well) were seeded in each well of 96 well flat-bottom tissue culture plate (Falcon #3072). The plate was allowed to stand for 2 hrs., thus the cells were adhered to the bottom of each well. To the each well was added 10 μl vehicle (1% DMSO), serial dilutions of test compounds in 1% DMSO, or 5nM Dexamethasone in 1% DMSO as a reference. The mixture (90μl/well) was incubated for 1 hr. at 37°C. After 1 hr., 1 μg/ml TNF-α (lOμl) in culture medium was added to the mixture to obtain lOOμl of reaction mixture. The reaction mixture was cultured for 24 hrs. to stimulate the cells with lOOng ml TNF-α. Cells with vehicle without TNF-α stimulation were also prepared.
(2) Enzyme immunoassay
Then the concentration of RANTES released from the cells in the supernatants of each well was determined using a quantitative sandwich enzyme immunoassay technique. First, 2μg/ml mouse anti-huRANTES mAb
(R&D Systems, #mAb678) in PBS buffer (pH 7.4, lOOμl) was put in each well of 96-well NUNC fluoro plate (Nalge Nunc, New York USA) (Final 200ng/well) and the plate was allowed to stand for overnight at 4°C to be coated by the antibody. Each well of the plate was then washed with 350μl wash buffer (0.05% Tween-20, 0.85% NaCl, and 25mM Tris HCl pH7.4) for three times. Blocking buffer containing 1% BSA (Sigma 99% pure, lOOg), 5% sucrose (Nacalai tesque, 99% pure, 500 g), and 0.02% azide (Nacalai tesque, 100%, 500 g) were added (200 μl) to each well and then the plate was allowed to stand for 4 hrs. to stabilize the coated antibody.
Next, 50μl supernatants of cell culture prepared in (1) above were put in each well of the 96-well NUNC fluoro plate with coated antibody. Recombinant Human RANTES (Pepro Tech, Inc. #300-06) was used as the standard for the determination of RANTES production (linear range between 1 and 10 ng/ml). Eu-labelled mouse anti-huRANES mAb (60ng/ml: R&D Systems, #mAb278) in PBS supplemented by 1% BSA and 0.05% Tween 20 was added (50μl) to each well. The reaction mixtures were incubated at room temperature for 4 hrs. After washing with wash buffer (0.05% Tween-20, 0.85% NaCl, and 25mM Tris/HCl pH7.4, 350μl/well) for 5 times with the use of a Sera Washer (Bio-Tech, #MW-96R), the enhancement solution (DELFIA, #1244-405, lOOμl/well) was added to each well. The plate was incubated for 10 minutes at room temperature with moderate shaking. Fluorescent intensity was measured using a DELFIA fluorimeter (Wallac). Excitation was performed at 340nm and emission was measured at 615nm.
The measurement of IL-2 production in Jurkat T cells in response to antibody stimulation.
IL-2 production was measured in Jurkat T cells (E6-1 clone; ATCC # TIB-152) in response to stimulation with anti-CD3/anti-CD28 antibodies.
(1) Preparation of immobilized antibodies
First, anti-CD3 antibodies (400 ng/well Nichirei, NU-T3 4μg/ml in lOOμl Dulbecco's PBS) were put in each well of 96-well plate (Falcon #3072) and the plate was allowed to stand for 2 hrs. at room temperature to be coated with the antibody. Each well of the plate was then washed with 250 μl PBS 3 times. (2) Preparation of Jurkat cell culture
Jurkat T cells were cultured in RPMI 1640 medium supplemented with 10% heat-inactivated fetal calf serum, 2 mM L-glutamine, 100 U/ml penicillin G, and lOOμg/ml streptomycin (culture medium). Two hundred thousand
(2xl05) cells (190μl/well) were seeded in each well of 96-well U-bottom tissue culture plates (Falcon #3077). To each well was added lOμl vehicle (0.2%) DMSO), serial dilution of compounds in 0.2% DMSO, or 25 nM cyclosporin A as a reference in 0.2% DMSO. The mixture (200μl) was incubated for 1 hr. at 37°C in a humidified 5% CO environment.
(3) Stimulation of the cell
The reaction mixture obtained in (2) (lOOμl) was put in the each well of the antibody-immobilized plate prepared in (1). To this well was added anti- CD28 antibodies (Nichirei, KOLT-2, 6 μg/ml in cell culture medium,
50 μl/well) and 2.5 μg/ml goat anti-mouse kappa chain antibodies (Bethyl Laboratories, (Cat#A90-119A) 10 μg/ml in culture medium, 50μl/well). The reaction mixture in each well was incubated for 24 hrs. at 37°C to stimulate cells with immobilized anti-CD3 antibodies (400ng/well) and anti-CD28 antibodies (1.5 μg/ml), and then to cross-link receptors on the cells with anti- mouse kappa chain antibodies (2.5 μg/ml).
(4) Measurement of IL-2 production
The supernatants of the reaction mixture were then collected. The IL-2 concentration in the supernatants was determined using a DuoSet™ ELISA
Development Kit (GenzymeTechne, Minneapolis, USA) following the manufacturer's recommendations. First, 2 μg/ml of mouse anti-huIL-2 Ab in
PBS buffer (lOOμl) was put in each well of 96-well plate (NUNC, Maxisorp
M) and the plate was allowed to stand for overnight at 4°C to be coated with the antibody. Each well of the plate was then washed 5 times with 350μl of wash buffer containing PBS, 0.05% Tween 20 (Nakalai tesque) using Sera Washer (Bio-Tech, #MW-96R). To each well was added 250μl of 1% BSA (Sigma) in PBS, 0.05% Tween 20 (dilution buffer). After 2 hrs. incubation at room temperature, the buffer was discarded, and 50μl of culture medium was added. Next, 50μl supernatant of stimulated cell culture prepared (3) above was put in each well of the 96-well plate with coated mouse anti-huIL-2 antibody. Recombinant Human IL-2 (Genzyme Techne) was used as the standard for the determination of IL-2 production (linear range between 200 and 5,400pg/ml). The reaction mixtures were incubated for 1 hr. at room temperature. After 5 times washing, lOOμl biotinylated rabbit anti-huIL-2 antibody (Genzyme Techne, 1.25 μg/ml) in dilution buffer was added to each well, and incubated at room temperature for 1 hr. After 5 times washing, lOOμl of Streptavidin-conjugated horseradishperoxidase (Genzyme Techne, 1/1000 in dilution buffer) was added to each well. After 20 minutes, each well of the plate was washed 5 times with wash buffer (350 μl/well). Substrate and H2O2 (TMBZ peroxidase detection kit, SUMILON #ML-
1120T) were added to the mixture and the mixture was allowed to stand at room temperature. The reaction was terminated after 10 minutes by adding 2N H2SO4. Optical density at 450nm was measured with the use of a microplate reader (Labosystems, Multiscan Multisoft). Quantification of IL-2 production in each sample was performed by comparison of optical densities between each sample and the standard curve.
Mouse LPS-induced TNF-α production
Eight weeks old BALB/c female mice were placed into two groups, a control group and a treated group. A solution containing 200μg/mouse of LPS in 0.9%) physiological salt was administered by intraperitoneal (i.p.) injection into the control mice. Mice in the treated group were first injected i.p. with compounds of the present invention 30 minutes prior to the LPS injection. Under anesthesia with pentobarbital (80mg/kg, i.p.), blood was collected from the posterior venous cavity) of the treated and control mice at 90 min post-LPS injection into 96-well plate containing 2% EDTA solution. The plasma was separated by centrifugation at 1800 rpm for 10 minutes at 4°C and then diluted with four times volumes of phosphate buffer saline (pH 7.4) containing 1% bovine serum albumin. TNF-α concentration in the sample was determined using an ELISA kit (Pharmingen, San Diego, CA.)
The mean TNF-α level in 5 mice from each group was determined and the percent reduction in TNF-α levels was calculated. The treated mice showed significant decrease in the level of TNF-α as compared to the control mice. The result indicates that the compounds of the present invention can restrain LPS-induced cytokine activity.
The results of a in vitro test and cellular assay tests (A549 and Jurkat) are shown in the tables in the Examples below. The data corresponds to the compounds as yielded by solid phase synthesis and thus to levels of purity of about 40 to 90%. For practical reasons, the compounds are grouped in four classes of activity as follows:
frι vitroIC5o= A 0.5μM < B 2 μM < C 10 μM < D Cellular IC50 =A 1 μM < B 10 μM < C
The compounds of the present invention also show excellent selectivity, and strong activity in vivo assays.
EXAMPLES
The present invention will be described in detail below in the form of examples, but they should by no means be construed as defining the metes and bounds of the present invention.
In the examples below, all quantitative data, if not stated otherwise, relate to percentages by weight. 1H NMR spectra were recorded using either Bruker DRX- 300 (300MHz for 1H) spectrometer in CDC13 . Chemical shifts are reported in parts per million (ppm) with tetramethylsilane (TMS) as an internal standard at zero ppm.
Coupling constant (J) are given in hertz and the abbreviations s, d, t, q, m, and br refer to singlet, doblet, triplet, quartet, multiplet, and broad, respectively. The mass determinations were carried out by MAT95 (Finnigan MAT).
Preparing method of starting compounds:
Starting compound 1A :2-Benzyloxyacetophenone
A mixture of 2'-hydroxyacetophenone (68. lg, 0.500mol), benzylbromide (65.4ml, 94. lg, 0.550mol) and K2CO3 (103g, 0.750mol) in acetone (1.00L) was stirred and heated to reflux overnight. The resulting mixture was concentrated under reduced pressure to obtain a residue. The obtained residue was dissolved in the mixture of AcOEt and water and then extracted with AcOEt. The extract was washed with brine, dried over magnesium sulfate, filtered, and concentrated under reduced pressure to give a crude material. The crude material was purified by distillation under reduced pressure to give starting compound 1A as a colorless oil (lOOg, yield 88%).
Starting compound 1A': l-{2-(cyclopropylmethoxy)-6-[(4-methoxybenzyl)oxy] phenyl} ethanone
To a stirred solution of l-(2,6-dihydroxyphenyl)ethanone (50.0 g, 328 mmol) in acetone (1000 mL) was added potassium carbonate (227 g, 1643 mmol) and (bromomethyl)cyclopropane (35.1 mL, 361 mmol). The mixture was stirred at 50°C for 2 days. The reaction mixture was filtrated on celite, and then the filtrate was concentrated under reduced pressure. The residue was diluted with water and extracted with ethyl acetate. The separated organic phase was washed with water and brine, dried over MgSO , filtered and concentrated under reduced pressure. The residue was suspended in hexane. Then the suspension was stirred at 80°C for 30 minuets. The solution was filtered and the filtrate was allowed to cool to room temperature. The resulting white solid was collected by filtration, washed with hexane, and dried under reduced pressure to give l-{2-[(cyclopropylmethyl)oxy] -6- hydroxyphenyl} ethanone as a pale yellow solid (56.3 g, yield; 83%).
To a stirred solution of l-{2-[(cyclopropylmethyl)oxy]-6-hydroxyphenyl} ethanone
(56.3 g, 272 mmol) in acetone (1000 mL) was added potassium carbonate (188 g, 1364 mmol), 4-methoxybenzyl chloride (40.9 mL, 300 mmol) and tetrabutyl- ammonium iodide (20.2 g, 54.6 mmol). The mixture was stirred at reflux overnight. The reaction mixture was allowed to cool to room temperature, filtered on Celite, and then the filtrate was concentrated under reduced pressure. The residue was diluted with water and extracted with ethyl acetate. The separated organic phase was washed with brine, dried over MgSO4, filtered and concentrated under reduced pressure. Then the resulting white solid was recrystallized from ethanol, collected by filtration, washed with ethanol, and dried under reduced pressure to give starting compound 1A' as a white solid (79.2 g, yield; 89%).
Starting compound IB :2'-[tert-butyI(dimethyl)silyl]oxyacetophenone
To a stirred solution of 2' -hydroxy acetophenone (10.00 g, 73.45 mmol) in DMF
(150 mL) were added imidazole (6.00 g, 88.14 mmol) and tert-butyl dimethylchloro- silane (12.18 g, 80.79 mmol). The mixture was stirred at room temperature for 60 hrs., and then diluted with diethyl ether. The resulting organic phase was washed with water, aqueous potassium hydrogen sulfate solution, and brine, successively.
Then the washed organic phase was dried over magnesium sulfate, filtered, and concentrated under reduced pressure. The residue was purified by column chromatography on silica-gel (hexane: ethyl acetate, 9:1 - 4:1) to give starting compound IB as a colorless oil (19.83 g, yield 92%).
Starting compound 1C :3-Benzyloxypicolinonitrile
A suspension of 3-hydroxypicolinonitrile (3.00 g, 25.0 mmol), which was prepared according to Synthesis 316 (1983) and J. Org. Chem. 48,1375(1983), potassium carbonate (5.40 g, 39.1 mmol) and benzyl bromide (5.10 g, 29.8 mmol) in acetone (150 mL) was stirred at room temperature for 20 hrs. The reaction mixture was filtrated and the filtrate was evaporated. The residue was purified by column chromatography on silica gel (ethyl acetate : n-hexane = 1:3 to 1:2) and recrystallization from ethyl acetate : n-hexane = 1:4 to give starting compound IC as a colorless powder (4.354g, yield 83%).
Starting compound ID :2-Acetyl-3-benzyloxypyridine
To a cold solution (0°C) of 3-benzyloxy-picolinonitrile (2.50 g, 11.9 mmol) in tetra- hydrofuran (100 mL) was added 0.92M methylmagnesium bromide in tetrahydro- furan (150 mL, 138 mmol) and stirred at 0°C for 30 minutes then at room temperature for 4 hrs. The reaction mixture was poured into water (2 L) and acidified by 10%) sulfuric acid (500 mL). After stirring for 30 minutes, the reaction mixture was added to sodium bicarbonate (100 g) slowly and extracted into ethyl acetate. The resulting organic phase was washed with saturated sodium chloride, dried anhydrous Na2SO4, filtrated, and evaporated. The residue was purified by column chromato- graphy on silica gel(ethyl acetate:n-hexane, 1:3) to give starting compound ID as a colorless oil (2.49 g, yield 92%). Starting compound 2 : 2-amino-4-(3-aminophenyl)-6-(2-{[fer/-butyl(dimethyl)- silyl] oxy}phenyl)nicotinonitrile
A mixture of 2'-[tert-butyl(dimethyl)silyl]-oxyacetophenone (starting compound IB, 20.0 g, 79.9 mmol), 3-nitrobenzaldehyde (12.1 g, 79.9 mmol), malononitrile (5.3 g, 79.9 mmol), ammomum acetate (9.2 g, 119.8 mmol) and toluene (25 mL) was stirred under reflux for 3 hrs. The reaction mixture was extracted with ethyl acetate and water. The separated organic phase was dried over Na2SO4, filtrated, and concentrated under reduced pressure. The crude product was purified by column chromatography on silica gel (hexane/ ethyl acetate, 9:1 - 4:1) to give 2-amino-6-(2- {[tert-butyl(dimethyl)silyl]-oxy}phenyl)-4-(3-nitrophenyl)nicotinonitrile as a white solid (6.5 g, 18%).
A mixture of 2-amino-6-(2-{[tert-butyl(dimethyl)-silyl]oxy}phenyl)-4-(3-nitro- ρhenyl)nicotinonitrile (3.2 g, 7.2 mmol), 10% Pd-C (0.25 g) and ethyl acetate (1 L) was stirred at room temperature for 12 hrs. under a hydrogen atmosphere (2.5 atm). The reaction mixture was filtered on Celite Pad and the filtration was concentrated under reduced pressure to give starting compound 2, which was used for the next step without further purification (2.6 g, yield 87%). Starting compound 3 :2-amino-4-[3-amino-4-(l-pyrrolidinyl)phenyl]-6-(2- benzyloxyphenyl)nicotinonitrile
A mixture of 2-benzyloxyacetophenone (5.00 g, 22.10 mmol), 4'-chloro-3'-nitro- benzaldehyde (8.20 g, 44.19 mmol), malononitrile (2.92 g, 44.19 mmol), and ammonium acetate (8.52 g, 110.48 mmol) in toluene (15 mL) was stirred at 130°C for 3 hrs. After cooled to room temperature, the mixture was diluted with ethyl acetate and THF. The organic phase was washed twice with water, dried over Na2SO4, filtered, and concentrated under reduced pressure. The residue was suspended in ethanol. The precipitate was collected by filtration, washed with ethyl acetate, and dried under reduced pressure to give 2-amino-6-(2-benzyloxyphenyl)-4- (4-chloro-3-nitrophenyl)nicotinonitrile as a white solid (6.40 g, yield 63%).
To a cold (0°C) solution of 2-amino-6-(2-benzyloxyphenyl)-4-(4-chloro-3-nitro- phenyl)nicotinonitrile (1.000 g, 2.189 mmol) in DMF (10 mL) was added pyrrolidine
(0.778 g, 10.943 mmol). The mixture was stirred at room temperature for 2hrs. and then at 60°C for 2 hrs. The reaction mixture was partitioned between ethyl acetate and water. The separated organic phase was washed with brine, dried over Na2SO4, filtered, and concentrated under reduced pressure. The residue was suspended in ethanol, and the precipitate was collected by filtration, washed with ethyl acetate and dried under reduced pressure to give 2-amino-6-(2-benzyloxyphenyl)-4-[3-nitro-4-(l- pyrrolidinyl)phenyl] nicotinonitrile as a yellow solid (0.990 g, yield 92%). To a suspension of 2-amino-6-(2-(benzyloxy)phenyl)-4-[3-nitro-4-(l-pyrrolidinyl)- phenyl]mcotinonitrile (1.000 g, 2.034 mmol) and Fe powder (3.000 g) in ethanol (180 mL) were added water (10 mL) and then ammonium chloride (1.000 g). The mixture was stirred under reflux for 3 hrs. The mixture was filtered on Celite Pad and the filtrate was concentrated under reduced pressure. The residue was suspended in ethanol, and the precipitate was collected by filtration, washed with ethyl acetate, and dried under reduced pressure to give starting compound 3 as a white solid (0.700 g, yield 75%).
Starting compound 4 :2-ammo-4-[3-amino-4-(dimethylamino)phenyl]-6-{2-[(4- methoxybenzyl)oxy]phenyl}nicotinonitriIe
A mixture of 2-(4-methoxybenzyloxy)acetophenone (5.663 g, 22.097mmol), 4'- chloro-3'-nitrobenzaldehyde (8.201 g, 44.193 mmol), malononitrile (2.920 g,
44.193 mmol), and ammonium acetate (8.516 g, 110.486 mmol) in toluene (15 mL) was stirred at 130°C for 4 hrs. The reaction mixture was partitioned between ethyl acetate and water. The separated organic phase was washed with brine, dried over Na SO , filtered, and concentrated under reduced pressure. The residue was suspended with ethanol. The resulting solid was collected by filtration, washed with ethanol, ethyl acetate and diethyl ether, and dried under reduced pressure to give 2- amino-4-(4-chloro-3-nitrophenyl)-6-{2-[(4-methoxybenzyl)oxy]phenyl}-nicotino- nitrile as a white solid (5.560 g, yield 52%). To a stirred solution of 2-amino-4-(4-chloro-3-nitrophenyl)-6-{2-[(4-methoxy- benzyl)oxy]phenyl}-nicotinonitrile (0.850 g, 1.746 mmol) in DMF (5 mL) including triethylamine (1.2 mL) was added dimethylamine hydrochloride (0.996 g, 12.220 mmol), and the mixture was stirred at 70°C for 3 hrs. The reaction mixture was quenched with water and extracted with ethyl acetate. The organic phase was washed with brine, dried over Na SO4, filtered, and concentrated under reduced pressure. The residue was suspended in ethanol. The resulting solid was collected by filtration, washed with ethanol, and dried under reduced pressure to give 2-amino- 4-[4-(dimethylamino)-3-nitrophenyl]-6-{2-[(4-methoxybenzyl)-oxy]phenyl}nicotino- nitrile as an orange oil (0.820 g, yield 95%).
To a stirred suspension of 2-amino-4-[4-(dimethylamino)-3-nitrophenyl]-6-{2-[(4- methoxybenzyl) oxy]phenyl}nicotinonitrile (0.815 g, 1.654 mmol) in ethanol (150 mL) were added Fe powder (5.000 g), water (20 mL) and ammonium chloride (1.000 g). The mixture was stirred under reflux for 12 hrs. The reaction mixture was cooled to room temperature, filtered on Celite Pad. The filtrate was concentrated under reduced pressure. The residue was suspended in ethanol. The resulting solid was collected by filtration, washed with ethanol and water, and dried under reduced pressure to give starting compound 4 as an orange solid (0.500 g, yield 65%).
Starting compound 5 :3-(2-ammo-3-cyano-6-{2-[(4-methoxybenzyl)oxy]phenyl}- 4-pyridinyI)benzoic acid
A mixture of 2-{(4-methoxybenzyl)oxy}acetophenone (0.26 g, 1.00 mmol), methyl 3-formbenzoate (0.16 g, 1.00 mmol), malononitrile (0.07 g, 1.00 mmol), and am- monium acetate (0.23 g, 3.00 mmol) in xylene (10 mL) was heated at 120°C (bath temperature) overnight. The resulting mixture was concentrated under reduced pressure, and the residue was diluted with water and extracted with ethyl acetate. The separated organic phase was washed with brine, dried over MgSO4, filtered, and concentrated under reduced pressure. The crude material was purified by column chromatography on silica gel (hexane: ethyl acetate, 70:30) to give 2-amino-6-{2-[(4- methoxybenzyl)oxy]phenyl} -4-(3-methoxycarbonylphenyl)-nicotinonitrile (0.200 g, yield 43%).
A mixture of 2-amino-6- {2-[(4-methoxybenzyl)-oxy]phenyl} -4-(3-methoxycarbonyl- ρhenyl)-nicotinonitrile (0.20 g, 0.43 mmol), 4N NaOH solution (1.0 mL), and THF (5.0 mL) was stirred at room temperature overnight. The reaction mixture was poured into water and extracted with ethyl ether twice. To the separated aqueous phase was added IN KHSO4 solution until the mixture became acidic. The resulting precipitate was collected by filtration and dried under reduced pressure to give the stating compound 5 as a gray powder (0.16 g, yield 81%).
Starting compound 6 :tert-butyl 2-amino-4-(3-aminophenyl)-6-(2-hydroxy- phenyI)nicotinate
A mixture of 2'-benzyloxyacetophenone (3.00 g, 13.25 mmol), 3'-nitrobenzaldehyde
(2.00 g, 13.25 mmol), tert-butyl cyanoacetate (1.87 g, 13.25 mmol), ammonium acetate (3.07 g, 39.77 mmol) in p-xylene (10 mL) was stirred at 120°C for 2 hrs. After cooled to room temperature, the reaction mixture was partitioned between ethyl acetate and water. The separated organic phase was washed with brine, dried over Na SO4, filtrated, and concentrated under reduced pressure. The crude product was purified by column chromatography on silica-gel (hexane: ethyl acetate, 5:1 - 2:1) to give tert-butyl 2-amino-6-[2-(benzyloxy)phenyl]-4-(3-nitrophenyl)nicotinate as a yellow oil (2.51 g, yield 38%).
A mixture of tert-butyl 2-amino-6-[2-(benzyloxy)-phenyl]-4-(3-nitrophenyl)- nicotinate (2.50 g, 5.02 mmol) and 10% Pd-C (0.20 g) in ethyl acetate (20 mL) was stirred at 2 atm under a hydrogen atmosphere for 5 hrs. The mixture was filtered on Celite Pad to remove Pd-C and washed with ethyl acetate. The filtrate was concentrated under reduced pressure, and the residue was purified by column chromatography on silica-gel (hexane: ethyl acetate, 4:1 - 3:2) to give starting compound 6 as a pale yellow oil (1.05 g, yield 56%).
Example 1-1
N-{3-[2-ammo-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl}-5-oxotetra- hydro-2-furancarboxamide
To a cold (0°C) mixture of pyridine (0.20 mL, 2.424 mmol), 2-amino-4-(3- aminophenyl)-6-(2-{[tert-butyl(dimethyl)silyl]oxy}phenyl)nicotinonitrile (starting compound 2, 1.010 g, 2.424 mmol), acetonitrile (10 mL) and THF (2 mL) was added dropwise a solution of 5-oxotetrahydro-2-furancarbonyl chloride (0.360 g, 2.424 mmol) in acetonitrile (4 mL). The mixture was stirred for 2 hrs., allowed to warm to room temperature, stirred for 1 hr., and extracted with ethyl acetate and water. The separated organic phase was washed with brine, dried over Na2SO , filtered and concentrated under reduced pressure. The residue was purified by column chromatography on silica-gel (hexane: Ethyl acetate, 7:3 - 5:5) to give N-{3- [2-amino-6-(2-{[tert-butyl(dimethyl)silyl]oxy}phenyl)-3-cyano-4-pyridinyl]phenyl}- 5-oxotefrahydro-2-furancarboxamide as a white solid (0.982 g, yield 77%).
To a cold (0°C) solution of N-{3-[2-amino-6-(2-{[tert-butyl(dimethyl)silyl]oxy}- phenyl)-3-cyano-4-pyridinyl]phenyl} -5-oxotetrahydro-2-furancarboxamide (0.400 g, 0.757 mmol) in THF (5 mL) was added drop wise a solution n-tetrabutylammonium fluoride in THF (1M, 2 mL). After being stirred for 30 minutes, the mixture was quenched with water (5 mL). The resulting precipitate was collected by filtration, washed with water and ethanol, and dried under reduced pressure to give N-{3-[2- amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl}-5-oxotetrahydro-2-furan- carboxamide as a white solid (0.293 g, yield 93%). Molecular weight: 414.424 Mass spectrometry: 415 Melting point: 155 dec
In vitro activity grade:B Cellular activity grade: (A954)-B
1H-NMR(300 MHz, DMSO-d6): 2.23 - 2.38 (2H, m), 2.55 - 2.59 (2H, m), 5.08 - 5.12 (1H, m), 6.87 - 6.93 (2H, m), 7.32 - 7.47 (4H, m), 7.50 - 7.56 (2H, m), 7.82 (1H, d, J = 8.3 Hz), 7.91 (1H, s), 8.02 (1H, d, J = 8.0 Hz), 10.47 (1H, s). Example 1-2
Sodium 5-({3-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]phenyI}- amino)-4-hydroxy-5-oxopentanoate
To a suspension of N-{3-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]- phenyl}-5-oxotetrahydro-2-furancarboxamide (0.250 g, 0.603 mmol) in methanol (10 mL) was added dropwise 2N NaOH solution (0.3 mL). After the mixture was stirred for 10 minutes, insoluble solid was filtered off. The filtrate was concentrated under reduced pressure and the residue was crystallized from methanol and ethyl ether. The resulting solid was collected by filtration under an argon atmosphere, washed with ethyl ether, and dried under reduced pressure to give Sodium 5-({3-[2- amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl}amino)-4-hydroxy-5-oxo- pentanoate as a pale yellow solid (0.165 g, yield 60%). Molecular weight: 454.421
Mass spectrometry: 433 Melting point: 199 dec In vitro activity grade: B Cellular activity grade: (A954)-C 1H-NMR (300 MHz, DMSO-d6): 1.72 - 1.98 (2H, m), 2.18 - 2.25 (2H, m), 4.01 (1H, dd, J = 3.2, 7.5 Hz), 6.60 - 6.85 (2H, m), 7.09 - 7.30 (3H, m), 7.32 (1H, d, J = 7.9 Hz), 7.46 (1H, t, J = 7.9 Hz), 7.67 (1H, br), 7.93 (1H, d, J = 8.2 Hz), 8.01 - 8.05 (2H, m), 9.91 (1H, s), 10.38 (1H, br s). Examples 1-03 to 1-23
In the similar manners as described in Example 1-01 and Example 1-02 above, compounds in Examples 1-03 to 1-23 as shown in Table 1 were synthesized.
Table 1
Example 2-1
N-{3-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl}-3-(l- piperidinyl)propanamide hydrochloride
To a cold (0°C) solution of 3-(l-ρiperidinyl)-propanoic acid (0.676 g, 3.601 mmol) in dichloromethane was added oxalyl chloride (0.471 mL, 5.401 mmol) and stirred at room temperature for 12 hrs. The mixture was concentrated under reduced pressure. The residue was added to the solution of 2-amino-4-(3-aminophenyl)-6-(2-{[tert- butyl(dimethyl)silyl]oxy}-phenyl)nicotinonitrile (starting compound 2, 1.500 g, 3.601 mmol) in acetonitrile (3 mL), and the resulting mixture was stirred at 0°C for 1 hr. The reaction mixture was extracted with ethyl acetate and water. The separated organic phase was washed with brine, dried over Na2SO , filtered and concentrated under reduced pressure. Purification was carried out by silica gel column chromatography (chloroform / methanol =10/1) to give N-{3-[2-amino-6-(2-{[tert-butyl(di- methyl)silyl]oxy}phenyl)-3-cyano-4-pyridinyl]phenyl}-3-(l-piperidinyl)propan- amide as a white solid (0.860g, yield 45%).
To a stirred solution of N-{3-[2-amino-6-(2-{[tert-butyl(dimethyl)silyl]oxy}phenyl)- 3-cyano-4-pyridinyl]-phenyl}-3-(l-piperidinyl)propanamide (0.150 g, 0.270 mmol) in 1,4-dioxane (5 mL) was added 4N HCl in dioxane (2 mL). The mixture was stirred at room temperature for 12 hrs. The resulting precipitate was collected by filtration, washed with ethanol, and dried under reduced pressure to give N-{3-[2- amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl}-3-(l-piperidinyl)propan- amide hydrochloride (0.01 lg, yield 9%).
Molecular weight: 477.998 Mass spectrometry: 442 In vitro activity grade: B Cellular activity grade: (A954)-B 1H-NMR(300 MHz, DMSO-d6): 1.38 - 1.50 (1H, m), 1.71 - 1.84 (5H, m), 2.76 -
3.04 (4H, m), 3.35 - 3.51 (4H, m), 6.90 - 6.98 (2H, m), 7.38 - 7.41 (3H, m), 7.54 (1H, t, J = 7.9 Hz), 7.83 (1H, d, J = 8.2 Hz), 7.95 (1H, s), 8.06 (1H, dd, J = 1.3, 8.2 Hz), 10.42 (1H, brs), 10.64 (1H, brs).
Examples 2-02 to 2-19
In the similar manners as described in Example 2-01 above, the compounds in Examples 2-02 to 2-19 as shown in Table 2 were synthesized.
Table 2
Example 3-1
N-[5-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-2-(l-pyrrolidinyl)- phenyl] -3-(l-piperidinyl)propanamide hydrochloride
To a stirred solution of 2-amino-4-[3-amino-4-(l-pyrrolidinyl)phenyl]-6-[2-(benzyl- oxyphenyl)]nicotinonitrile (starting compound 3, 0.166 g, 0.360 mmol) in pyridine (3 mL) was added 3-(l-piperidinyl)propanoyl chloride hydrochloride (0.153 g, 0.719 mmol) at room temperature and the stirring was continued for 3 hrs. The reaction mixture was extracted with ethyl acetate and water. The separated organic phase was washed with brine, dried over Na2SO4, filtered, and concentrated under reduced pressure. The residue was suspended in ethyl acetate and ethanol, and then the precipitate was collected by filtration, washed with ethanol, and dried under reduced pressure to give N-[5-[2-ammo-6-(2-benzyloxyphenyl)-3-cyano-4- pyridinyl]-2-(l -pyrrolidinyl) phenyl]-3-(l-piperidinyl)propanamide as a white solid
(0.070 g, yield 32%).
A mixture of N-[5-[2-amino-6-(2-benzyloxyphenyl)-3-cyano-4-pyridinyl]-2-(l-pyr- rolidinyl)phenyl]-3-(l-piperidinyl)propanamide (0.140 g, 0.233 mmol), 10%Pd-C (0.150 g), ethyl acetate, and acetic acid (2.000 mL) was stirred at room temperature under a hydrogen atmosphere for 12 hrs. The reaction mixture was filtered on Celite Pad and the filtrate was concentrated under reduced pressure. The residue was dissolved in THF (3 mL) and 4N HCl in 1,4-dioxane (0.5 mL) was added to the solution. The resulting yellow solid was collected by filtration, washed with THF, and dried under reduced pressure to give N-[5-[2-amino-3-cyano-6-(2-hydroxy- phenyl)-4-pyridinyl]-2-(l-pyrrolidinyl)phenyl]-3-(l-piperidinyl)propanamide hydro- chloride(0.110 g, yield 86%). Molecular weight: 547.105 Mass spectrometry: 511 Melting point: 152
In vitro activity grade: B
Cellular activity grade: (A954)-B
1H-NMR(300 MHz, DMSO-d6): 1.35 - 1.40 (1H, m), 1.67 - 1.80 (6H, m), 1.92 (4H, s), 2.89 - 2.98 (4H, m), 3.29 - 3.57 (8H, m), 3.58 - 3.60 (1H, m), 6.87 - 6.95 (2H, m), 7.02 (1H, d, J = 7.9 Hz), 7.30 - 7.37 (3H, m), 7.53 - 7.56 (2H, m), 7.98 (2H, d, J = 7.2
Hz), 9.85 (1H, s), 10.45 (1H, brs). Examples 3-02 to 3-17
In the similar manners as described in Example 3-01 above, the compounds in
Examples 3-02 to 3-17 as shown in Table 3 were synthesized.
Table 3
Example 4-1
N1-[5-[2-amino-3-cyano-6-(2-hydroxyphenyI)-4-pyridinyI]-2-(dimethylamino)- phenyl] -N -(cyclopropylmethyl)glycinamide hydrochloride
To a cooled (0°C), stirred solution of 2-amino-4-[3-amino-4-(dimethylamino)- phenyl]-6-{2-[(4-methoxybenzyl)-oxy]phenyl}nicotinonitrile (starting compound 4, 0.500 g, 1.724 mmol) in THF(7 mL) under an argon atmosphere was added chloro- acetyl chloride (0.121 g, 1.074 mmol). The mixture was stirred at 0°C for 15 minutes and then partitioned between ethyl acetate and saturated aqueous NaHCO3. The separated organic phase was washed with brine, dried over Na SO4, filtered, and concentrated under reduced pressure. The residue was suspended in ethyl acetate and ethanol. The resulting solid was collected by filtration, washed with ethanol, and dried under reduced pressure to give N-[5-(2-amino-3-cyano-6-{2-[(4-methoxy- benzyl)oxy]-phenyl} -4-pyridinyl)-2-(dimethylamino)phenyl]-2-chloroacetamide as a yellow solid. (0.410 g, yield 70%) To a stirred solution of N-[5-(2-amino-3-cyano-6-{2-[(4-methoxybenzyl)oxy]- phenyl} -4-pyridinyl)-2-(dimethylamino)phenyl]-2-chloroacetamide (0.400 g,
0.738 mmol) in DMF (5 mL) was added aminomethylcyclopropane (0.192 mL, 2.214 mmol). The mixture was stirred at 60°C for 2 hrs. After cooled to room temperature, the mixture was extracted with ethyl acetate and water. The separated organic phase was washed with brine, dried over Na2SO4, filtered and concentrated under reduced pressure. Purification was carried out by column chromatography on silica gel (chloroform: methanol = 100:2, hexane:ethyl acetate=l:2-l:3-l:5, 2 times) to give N1-[5-(2-amino-3-cyano-6-{2-[(4-methoxybenzyl)oxy]phenyl}-4-pyridinyl)- 2-(dimethylamino)phenyl]-N2-(cyclopropylmethyl)-glycinamide as a yellow oil
(0.130 g, yield 30%).
To a suspension of N1-[5-(2-amino-3-cyano-6-{2-[(4-methoxybenzyl)oxy]phenyl}-4- pyridinyl)-2-(dimethylamino)-phenyl]-N2-(cyclopropylmethyl)glycinamide (0.130 g, 0.448 mmol) in anisole (1 mL) and water (1 mL) was added trifluoroacetic acid
(5 mL). The mixture was stirred at room temperature for 20 hrs. The reaction mixture was diluted with toluene and concentrated under reduced pressure. The residue was suspended in hexane and the precipitate was collected by filtration. The solid obtained was dissolved in THF and 4N HCl in 1,4-dioxane was added to the solution. The resulting solid was collected by filtration, washed with THF and 1,4- dioxane, and dried under reduced pressure to give N1-[5-[2-amino-3-cyano-6-(2-hy- droxyphenyl)-4-pyridinyl]-2-(dimethylamino)phenyl]-N2-(cyclopropylmethyl)glycin- amide hydrochloride as a yellow solid (0.085 g, yield 77%). Molecular weight: 493.013 Mass spectrometry: 457
In vitro activity grade: B Cellular activity grade: (A954)-B
1H-NMR(300 MHz, DMSO-d6): 0.35 - 0.40 (2H, m), 0.57 - 0.63 (2H, m), 1.05 - 1.08 (1H, m), 2.80 (6H, s), 2.87 - 2.91 (2H, m), 4.66 - 4.68 (2H, m), 6.87 - 6.94 (2H, m), 7.33 - 7.40 (4H, m), 7.52 - 7.55 (1H, m), 7.98 (1H, d, J = 7.2 Hz), 8.09 (1H, s),
9.12 (1H, brs), 10.07 (1H, brs). Examples 4-02 to 4-39
In the similar manners as described in Example 4-01 above, the compounds in Examples 4-02 to 4-39 as shown in Table 4 were synthesized.
Table 4
Example 5-1
Sodium 3-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]benzoate
A mixture of 3-(2-amino-3-cyano-6-{2-[(4-methoxybenzyl)oxy]ρhenyl}-4- pyridinyl)benzoic acid (starting compound 5, 0.16 g, 0.36 mmol), trifluoroacetic acid (3.0 mL), anisole (0.50 mL), and water (0.50 mL) was stirred at room temperature overnight. The reaction mixture was diluted with toluene and concentrated under reduced pressure. The residue was dissolved in THF and then crystallized with hexane. The resulting precipitate was collected by filtration and washed with hexane, and then dried under reduced pressure to give 2-amino-6-(2-hydroxyphenyl)-
4-(3-hydroxycarbonylphenyl)nicotinonitrile (0.12 g, yield 74%).
2-amino-6-(2-hydroxyphenyl)-4-(3-hydroxycarbonyl-phenyl)nicotinonitrile (0.02 g,
0.045 mmol) was dissolved in 4N NaOH solution (2 mL), and the resulting solution was purified by HPLC (ODS reverse phase column, 10% CH3CN/H2O - 90%
CH3CN/H O) to give Sodium 3-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4- pyridinyl]benzoate as a pale yellow powder (0.015 g, 95%).
Molecular weight: 353.316
Mass spectrometry: 332 Melting point: >270
In vitro activity grade: C
Cellular activity grade: (A954)-C
1H-NMR(300 MHz, DMSO-d6): 6.85 - 6.93 (2H, m), 7.31 - 7.56 (5H, m), 7.58 (1H, d, J = 3.0 Hz), 7.99 - 8.06 (2H, m), 8.10 (1H, s), 13.40 (1H, br s).
Examples 5-02 to 5-05
In the similar manners as described in Example 5-01 above, the compounds in Examples 5-02 to 5-05 as shown in Table 5 were synthesized.
Table 5
Example 6-1
3-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-N-[2-(l-piperidinyl)- ethyl]benzamide trifluoroacetate
To a cold (0°C) mixture of 3-(2-amino-3-cyano-6-{2-[(4-methoxybenzyl)oxy]- phenyl}-4-pyridinyl)benzoic acid (starting compound 5, 1.35g, 3.00 mmol), N-(2- aminoethyl)piperidine (0.420g, 3.30 mmol), and 1-hydroxyberrzotriazole (0.490g, 3.60 mmol) was added l-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydro- chloride (0.69 g, 3.60 mmol) with stirring. The mixture was allowed to warm to room temperature and stirred overnight. The reaction mixture was concentrated under reduced pressure. The residue was dissolved in ethyl acetate and partitioned between ethyl acetate and water. The separated organic phase was washed with brine, dried over MgSO4, filtered and concentrated under reduced pressure. The obtained solid was triturated with ethyl ether and dried under reduced pressure to give 2-(l-piperidinyl)ethyl 3-(2-amino-3-cyano-6-{2-[(4-methoxybenzyl)oxy]- phenyl}-4-pyridinyl)benzoate (0.98 g, yield 58%>).
A mixture of 2-(l-piperidinyl)ethyl 3-(2-amino-3-cyano-6-{2-[(4-methoxybenzyl)- oxy]phenyl}-4-pyridinyl)-benzoate (0.14 g, 0.25 mmol), trifluoroacetic acid (3.00 mL), anisole (0.50 mL) and water (0.50 mL) was stirred at room temperature overnight. The reaction mixture was diluted with toluene and concentrated under reduced pressure. The residue was purified by HPLC (ODS reverse phase column, 10% CH3CN/H2O - 90% CH3CN/H2O) to give 3-[2-amino-3-cyano-6-(2-hydroxy- phenyl)-4-pyridinyl]-N-[2-(l-piperidinyl)ethyl]benzamide trifluoroacetate as a pale yellow solid (0.061 g, yield 44%).
Molecular weight: 555.562
Mass spectrometry: 442
Melting point: 131 dec
In vitro activity grade: C
Cellular activity grade: (A954)-B
1H-NMR(300 MHz, DMSO-d6): 1.36 - 1.86 (6H, m), 2.95 (2H, q, J = 10.5 Hz), 3.26
- 3.28 (2H, m), 3.56 (2H, d, J = 12.4 Hz), 3.66 (2H, J = 6.6 Hz), 6.88 - 6.95 (2H, m),
7.01 (IH, t, J = 7.91 Hz), 7.45 (IH, s), 7.53 (2H, s), 7.70 (IH, t, J = 7.9 Hz), 7.88 (IH, d, J = 7.54 Hz), 8.05 (2H, t, J = 7.0 Hz), 8.12 (IH, s), 8.84 (IH, t, J = 5.7 Hz),
9.02 (lH, br_s), 13.31 (lH, s).
Examples 6-02 to 6-03 In the similar manners as described in Example 6-01 above, compounds in Examples
6-02 and 6-03 as shown in Table 6 were synthesized.
Table 6
Example 7-1
Sodium 2-amino-4-(3-aminophenyl)-6-(2-hydroxyphenyl)nicotinate
A mixture of tert-butyl 2-amino-4-(3-aminophenyl)-6-(2-hydroxyphenyl)nicotinate (starting compound 6, 0.025 g, 0.066 mmol) and trifluoroacetic acid (1 mL) was stirred at room temperature for 2 hrs. After the mixture was concentrated under reduced pressure, the residue was dissolved in acetonitrile (0.5 mL) and then treated with saturated NaHCO3 solution (0.7 L). The resulting mixture was purified by HPLC (ODS reverse phase column, 10% CH3CN/H2O - 90% CH3CN/H2O) to give Sodium 2-amino-4-(3-aminophenyl)-6-(2-hydroxyphenyl)nicotinate as a pale yellow solid (0.020 g, yield 88%). Molecular weight: 343.320 Mass spectrometry: 322 Melting point: 140 dec In vitro activity grade: C
Cellular activity grade: (A954)-D
1H-NMR(300 MHz, DMSO-d6): 4.94 (2H, s), 6.50 (IH, dd, J = 1.1, 7.9 Hz), 6.63 (IH, d, J = 7.5 Hz), 6.68 (IH, br), 6.73 (2H, s), 6.77 - 6.83 (2H, m), 6.95 (IH, d, J - 7.9 Hz), 6.98 (IH, s), 7.19 (IH, dt, J = 1.5, 8.3 Hz), 7.81 (IH, dd, J = 1.1, 7.9 Hz), 14.64 (IH, s). Example 7-2
Sodium 5-({3-[2-amino-3-(aminocarbonyl)-6-(2-hydroxyphenyl)-4-pyridinyl]- phenyl}amino)-4-hydroxy-5-oxopentanoate
To a cold (0°C) solution of tert-butyl 2-amino-4-(3-aminophenyl)-6-(2-hydroxy- phenyl)nicotinate (starting compound 6, 0.626 g, 1.659 mmol) in THF (7 mL) including pyridine (0.148 mL, 1.824 mmol) was added a solution of 5-oxotetrahydro-2- furancarbonyl chloride (0.271 g, 1.824 mmol) in THF (3 mL). After 1 hr., the mixture was allowed to warm to room temperature and the stirring was continued for 2 hrs. The mixture was partitioned between ethyl acetate and water. The separated organic phase was washed with brine, dried over Na SO4, filtered, and concentrated under reduced pressure. The residue was purified by column chromatography on silica-gel (hexane: ethyl acetate, 1:4 - 1:19) to give tert-butyl 2-amino-6-(2-hydroxy- phenyl)-4-(3 - { [(5-oxotetrahydro-2-furanyl)carbonyl] amino } -phenyl)-nicotinate as a pale yellow foam (0.677 g, yield 83%).
A mixture of tert-butyl 2-amino-6-(2-hydroxyphenyl)-4-(3-{[(5-oxotetrahydro-2- furanyl)carbonyl] amino }-phenyl)nicotinate (0.025 g, 0.051 mmol) in trifluoroacetic acid (1 mL) was stirred at room temperature for 2 hrs. After the mixture was concen- trated under reduced pressure, the residue was dissolved in acetonitrile (0.6 mL) and then treated with saturated NaHCO3 solution (0.2 mL). The resulting mixture was purified by HPLC (ODS reverse phase column, 10% CH3CN/H2O - 90% CH3CN/H2O) to give sodium 2-amino-6-(2-hydroxyphenyl)-4-(3-{[(5-oxotetrahydro- 2-fi_ιranyl)carbonyl]amino}phenyl)nicotinate as a pale yellow solid (0.009 g, yield 39%).
To a cold (0°C) mixture of 2-amino-6-(2-hydroxyphenyl)-4-(3-{[(5-oxotetrahydro-2- furanyl)carbonyl]-amino}phenyl)nicotinic acid (0.070 g, 0.15 mmol), ammonium chloride (0.016 g, 0.30 mmol), 1-hydroxybenzotriazole hydrate (0.032 g, 0.24 mmol) in DMF (2 mL) was added l-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (0.034 g, 0.18 mmol) followed by triethylamine (0.042 mL, 0.30 mmol). After 1 hr., the mixture was allowed to warm to room temperature and the stirring was continued overnight. The resulting mixture was diluted with water and extracted with ethyl acetate. The separated organic phase was washed with brine, dried over Na SO , filtered and concentrated under reduced pressure. The residue was purified by HPLC (ODS reverse phase column, 50% CH3CN/H2O - 90% CH3CN/H2O) to give a carboxamide analog of the starting material. The product was dissolved in acetonitrile (0.7 mL) and then treated with IN NaOH solution (0.6 mL).
The resulting mixture was purified by HPLC (ODS reverse phase column, 10% CH3CN/H2O - 90% CH3CN H2O) to give Sodium 5-({3-[2-amino-3-(amino- carbonyl)-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl}amino)-4-hydroxy-5-oxopentan- oate as a yellow solid (0.005 g, yield 7%>). Molecular weight: 472.437
Mass spectrometry: 451 Melting point: >260 In vitro activity grade: C Cellular activity grade: (A954)-C 1H-NMR(300 MHz, DMSO-d6): 1.72 - 1.96 (2H, m), 2.11 - 2.20 (2H, m), 3.99 (IH, d, J = 6.8 Hz), 6.46 (2H, br), 6.82 (2H, br), 7.17 (IH, d, J = 7.8 Hz), 7.18 (4H, br), 7.34 (IH, t, J = 7.8 Hz), 7.84 (IH, s), 7.87 (IH, d, J = 8.0 Hz), 7.95 (IH, dd, J = 1.3, 8.0 Hz), 9.75 (IH, s), 10.65 (IH, s), 13.91 (IH, s). Example 7-3
N-{3-[2-amino-3-(hydroxymethyl)-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl}-3-
(l-piperidinyl)propanamide
To a cold (0°C) suspension of lithium aluminum hydride (0.077 g, 1.62 mmol) in THF (3 mL) under an argon atmosphere was added dropwise a solution of tert-butyl 2-amino-4-(3-aminophenyl)-6-(2-hydroxyphenyl)nicotinate (0.353 g, 0.935 mmol) in THF (2 mL) with stirring. After 30 minutes, the mixture was allowed to warm to room temperature, and the stirring was continued at room temperature for 2hrs. and at 50°C overnight. After cooled to room temperature, the reaction mixture was quenched with water and extracted with ethyl acetate. The separated organic phase was washed with brine, dried over Na SO4, filtered, and concentrated under reduced pressure. The residue was purified by recrystallization from methanol to give 2-[6- amino-4-(3-aminophenyl)-5-(hydroxymethyl)-2-pyridinyl]phenol as a pale yellow solid (0.133 g, yield 45%).
To a cold (0°C) solution of 2-[6-amino-4-(3-aminophenyl)-5-(hydroxymethyl)-2- pyridinyl]phenol (0.100 g, 0.325 mmol) in THF (3 mL) including pyridine (0.028 mL, 0.34 mmol) was added 3-chloropropionyl chloride (0.043 g, 0.34 mmol). After being stirred for 30 minutes, the mixture was allowed to warm to room temperature and the stirring was continued for 2 hrs. The resulting mixture was diluted with water and extracted with ethyl acetate. The separated organic phase was washed with brine, dried over Na2SO , filtered, and concentrated under reduced pressure. The residue was crystallized with ethyl ether. The resulting solid was collected by filtration, washed with ethyl ether, and dried under reduced pressure to give N- {3-[2-amino-3-(hydroxymethyl)-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl} -3- chloropropanamide as a pale yellow solid (0.072 g, yield 56%).
To a solution of N-{3-[2-amino-3-(hydroxymethyl)-6-(2-hydroxyphenyl)-4-pyridi- nyl]phenyl}-3-chloropropanamide (0.065 g, 0.16 mmol) and sodium iodide (0.007 g, 0.05 mmol) in acetonitrile (5 mL) was added piperidine (0.16 mL, 1.63 mmol), and the mixture was stirred at 75°C overnight. After cooled to room temperature, the resulting mixture was diluted with water and extracted with ethyl acetate. The sepa- rated organic phase was washed with brine, dried over Na2SO , filtered and concentrated under reduced pressure. The residue was purified by column chromatography on silica-gel (chloroform: methanol, 85:15) to give N-{3-[2-amino-3- (hydroxymethyl)-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl}-3-(l-piperidinyl)propa- na ide as a white foam (0.055 g, yield 75%). Molecular weight: 446.554
Mass spectrometry: 447 Melting point: 133dec In vitro activity grade: B Cellular activity grade: (A954)-B 1H-NMR(300 MHz, DMSO-d6): 1.36 - 1.55 (6H, m), 2.39 - 2.65 (8H, m), 4.34 (2H, d, J = 4.9 Hz), 5.08 (IH, t, J = 4.9 Hz), 6.49 (2H, s), 6.78 (IH, dt, J = 1.1, 8.3 Hz), 6.85 (IH, dd, J = 1.5, 8.67 Hz), 7.10 (IH, s), 7.14 (IH, d, J = 7.9 Hz), 7.23 (IH, dt, J - 1.5, 8.7 Hz), 7.40 (IH, t, J = 7.9 Hz), 7.61 (IH, s), 7.66 (IH, d, J = 8.3 Hz), 7.86 (IH, dd, J = 1.5, 8.3 Hz), 10.28 (IH, s), 14.22 (IH, s). Example 8-1
2-[6-amino-4-[4-(ethyIammo)-3-hydroxyphenyl]-5-(hydroxymethyi)-2- pyridinyl]-3-(cycloproylmethoxy)phenol hydrochloride
A mixture of 1 - {2-(cyclopropylmethoxy)-6- [(4-methoxybenzyl)oxy]phenyl} ethanone
(starting compound 1-A' 2.00 g, 6.13 mmol), 3-hydroxy-4-nitrobenzaldehyde (2.05 g, 12.26 mmol), tert-butyl cyanoacetate (1.73 g, 12.26 mmol), ammonium acetate (1.42 g, 18.38 mmol) and 1,2-dimethoxy ethane (2.0 mL) was placed in a sealed tube and was stirred at 100°C for 8 hrs. After being cooled to room temperature, the mixture was concentrated under reduced pressure, and the residue was partitioned between ethyl acetate and water. The separated organic phase was washed with brine, dried over Na SO4, filtered, and concentrated under reduced pressure. The residue was purified by flash chromatography on silica gel (hexane: ethyl acetate, 1:1) to give tert-butyl 2-amino-6-{2-(cyclopropylmethoxy)-6-[(4- methoxybenzyl)oxy]phenyl}-4-(3-hydroxy-4-nifrophenyl)nicotinate. (2.32 g, 62%>);
LC-MS (m/z= 614, M+l)
A solution of tert-butyl 2-amino-6-{2-(cyclopropylmethoxy)-6-[(4-methoxybenzyl)- oxy]phenyl}-4-(3-hy<froxy-4-nitrophenyl)nicotinate (2.32 g, 3.78 mmol) in ethyl acetate (15.0 mL) and THF (15.0 mL) was hydrogenated at 1 atm in the presence of palladium on charcoal (10%, 0.10 g) overnight. The resulting mixture was filtered on Celite and washed with ethyl acetate and THF. The combined filtrate was concentrated under reduced pressure to give tert-butyl 2-amino-4-(4-amino-3-hy- droxyphenyl)-6-[2-[(4-methoxybenzyl)oxy]-6-(2-methylbutoxy)phenyl]nicotinate. (2.21 g, 100%); LC-MS (m/z = 584, M+l)
To a solution of tert-butyl 2-amino-4-(4-amino-3-hydroxyphenyl)-6-[2-[(4-methoxy- benzyl)oxy]-6-(2-methylbutoxy)phenyl]nicotinate (2.46 g, 4.21 mmol) was added acetic anhydride (0.44 mL, 4.63 mmol). The mixture was stirred at room temperature for overnight. The mixture was diluted with ethyl acetate and washed with saturated aqueous NaHCO3 and brine. The organic phase was dried over Na2SO4, filtered and concentrated under reduced pressure to give tert-butyl 4-[4- (acetylamino)-3-hydroxyphenyl]-2-amino-6- {2-(cyclopropylmethoxy)-6-[(4- methoxybenzyl)oxy]phenyl}nicotinate. (2.65 g, 100%); LC-MS (M/z = 626, M+l)
To a cold (0 °C) solution of tert-butyl 4-[4-(acetylamino)-3-hydroxyphenyl]-2- amino-6-{2-(cyclopropylmethoxy)-6-[(4-methoxybenzyl)oxy]phenyl}nicotinate (1.00 g, 1.60 mmol) in THF (15.0 mL) under argon atmosphere was added a solution of sodium bis(2-methoxyethoxy)aluminum hydride (3.7 N in toluene, 1.25 mL, 6.39 mmol) in THF (2 mL) dropwise. After being stirred for 5 hrs., a solution of sodium bis(2-methoxyethoxy)aluminum hydride (3.7 N in toluene, 0.833 mL, 4.26 mmol) in THF (1 mL) was added to the mixture at 0°C, then the mixture was allowed to warm to room temperature, and the stirring was continued overnight. The mixture was quenched with ethyl acetate and poured into saturated aqueous sodium potassium tartarate. The extracted organic phase was washed with brine, dried over Na SO4, filtered and concentrated under reduced pressure to give 5-[2-amino-6-{2- (cyclopropylmethoxy)-6-[(4-methoxybenzyl)oxy]phenyl}-3-(hydroxymethyl)-4- pyridinyl]-2-(ethylamino)ρhenol.; LC-MS (m/z = 542 M+l)
To a solution of 5-[2-amino-6-{2-(cyclopropylmethoxy)-6-[(4-methoxybenzyl)oxy]- phenyl}-3-(hydroxymethyl)-4-pyridinyl]-2-(ethylamino)phenol (0.013 g,
0.023 mmol) in 1,4-dioxane (0.5 mL) was added a solution of HCl in 1,4-dioxane (4N, 1.0 mL). After being stirred overnight, the mixture was diluted with diethyl ether. The resulting precipitate was collected by filtration under argon atmosphere, washed with Et2O, and dried under reduced pressure to give 2-[6-amino-4-[4- (ethylamino)-3-hydroxyphenyl]-5-(hydroxymethyl)-2-pyridinyl]-3- (cycloρroylmethoxy)ρhenol. (0.008 g, 76%); LC-MS (m/z = 422 M+l) Molecular weight: 457.961 Mass spectrometry: 422
Melting point: 232dec In vitro activity grade:B Cellular activity grade: (A954)-
1H-NMR(500 MHz, DMSO-d6): 0.26 (2H, m), 0.47 (2H,m), 1.13 (IH, m), 1.20 (3H, t, J = 7.3 Hz), 3.19 (2H, m), 3.57 (IH, s), 3.86 (2H, m), 4.51 (2H, s), 6.64 (2H, m),
6.82 (IH, s), 6.94 (2H, m), 7.28 (2H, m), 10.3 (IH, br), 13.3 (IH, br).
Example 8-2
7-[2-(cyclopropylmethoxy)-6-hydroxyphenyl-5-(3-ethyl-2-oxo-2,3-dihydro-l,3- benzoxazol-6-yl)-l,4-dihydro-2H-pyrido[2,3-d][l,3]oxazin-2-one hydrochloride
To a cold (0 °C) solution of 5-[2-amino-6-{2-(cyclopropylmethoxy)-6-[(4-methoxy- benzyl)oxy]phenyl}-3-(hydroxymethyl)-4-pyridinyl]-2-(ethylamino)phenol (0.150 g, 0.28 mmol) in THF (5.0 mL) including triethylamine (0.12 mL, 0.83 mmol) was added triphosgene (0.099 g, 0.33 mmol). After being stirred for 30 minutes, the mixture was allowed to warm to room temperature, and the stirring was continued overnight. The resulting mixture was quenched with water and extracted with ethyl acetate. The separated organic phase was washed with brine, dried over Na2SO4, filtered and concentrated under reduced pressure. The residue was purified by column chromatography on silica gel (dichloromethane: methanol, 9:1) to give 7-{2- (cyclopropyhnethoxy)-6-[(4-methoxybenzyl)oxy]phenyl}-5-(3-ethyl-2-oxo-2,3-di- hydro-l,3-benzoxazol-6-yl)-l,4-dihydro-2H-pyrido[2,3-d][l,3]oxazin-2-one. (0.049 g, 30%); LC-MS (m/z = 594, M+l)
To a solution of 7-{2-(cyclopropylmethoxy)-6-[(4-methoxybenzyl)oxy]phenyl}-5-(3- ethyl-2-oxo-2,3-dihydro-l,3-benzoxazol-6-yl)-l,4-dihydro-2H-ρyrido[2,3-d][l,3]- oxazin-2-one (0.036 g, 0.065 mmol) in 1,4-dioxane (1.0 mL) was added a solution of HCl in 1,4-dioxane (4N, 1.0 mL). After being stirred overnight, the mixture was diluted with diethyl ether. The resulting precipitate was collected by filtration under argon atmosphere, washed with Et2O, and dried under reduced pressure to give 7-[2- (cyclopropylmethoxy)-6-(hydroxy)phenyl-5-(3-ethyl-2-oxo-2,3-dihydro-l,3- benzoxazol-6-yl)-l,4-dihydro-2H-pyrido[2,3-d][l,3]oxazin-2-one. (0.025 g, 80%); LC-MS (m/z = 436, M+l) Molecular weight: 509.951
Mass spectrometry: 474 Melting point: 151 In vitro activity grade: C Cellular activity grade: (A954)- 1H-NMR(500 MHz, DMSO-d6): 0.29 (2H, m), 0.49 (2H, m), 1.20 (IH, m), 1.30 (3H, t, J = 7.3 Hz), 3.87 (2H, d, J = 6.5 Hz), 3.92 (2H, m), 5.43 (2H, s), 6.55 (2H, d, J = 8.2 Hz), 6.92 (0.5 H, d, J = 8.5 Hz), 7.19 (IH, t, J = 8.2 Hz), 7.33 (2H, m), 7.49 (2H, m), 7.97 (IH, s).
Example 8-3 N-{4-[2-amino-6-[2-(cyclopropylmethoxy)-6-hydroxyphenyl]-3-(hydroxy- methyl)-4-pyridinyl]-2-hydroxyplιenyl}acetamide lιydrochloride
To a cold (0 °C) solution of tert-butyl 4-[4-(acetylamino)-3-hydroxyphenyl]-2- amino-6-{2-(cyclopropylmethoxy)-6-[(4-methoxybenzyl)oxy]phenyl}nicotinate (1.65 g, 2.64 mmol) in THF (25.0 mL) under argon atmosphere was added a solution of sodium bis(2-methoxyethoxy)aluminum hydride (3.7 N in toluene, 4.12 mL, 21.1 mmol) in THF (3 mL) dropwise. After being stirred for 30 minutes, the mixture was quenched with ethyl acetate and poured into saturated aqueous sodium potassium tartarate. The extracted organic phase was washed with brine, dried over Na2SO4, filtered and concentrated under reduced pressure. The residue was purified by column chromatography on silica gel (dichloromethane: methanol, 19:1 - 9:1) to give N- {4-[2-amino-6- {2-(cyclopropylmethoxy)-6-[(4-methoxybenzyl)oxy]phenyl} - 3 -(hydroxymethyl)-4-pyridinyl]-2-hydroxyphenyl} acetamide (0.48 g, 33%); LC-MS
(m/z = 556, M+l).
To a solution of N-{4-[2-amino-6-{2-(cyclopropylmethoxy)-6-[(4-methoxybenzyl)- oxy]phenyl} -3-(hydroxymethyl)-4-pyridinyl]-2-hydroxyphenyl} acetamide (0.013 g, 0.023 mmol) in 1,4-dioxane (0.5 mL) was added a solution of HCl in 1,4-dioxane
(4N, 1.0 mL). After being stirred overnight, the mixture was diluted with diethyl ether. The resulting precipitate was collected by filtration under argon atmosphere, washed with Et2O, and dried under reduced pressure to give of N-{4-[2-amino-6-[2- (cyclopropylmethoxy)-6-hydroxyphenyl] -3 -(hydroxymethyl)-4-pyridinyl] -2- hydroxyphenyl} acetamide. (0.025 g, 80%); LC-MS (m/z = 436, M+l)
Molecular weight: 471.945
Mass spectrometry: 436
Melting point: 270 dec
In vitro activity grade: A
Cellular activity grade: (A954)-
1H-NMR(500 MHz, DMSO-d6): 0.27 (2H, m), 0.47 (2H, m), 1.14 (IH, m), 2.14 (3H, s), 3.86 (2H, d, J = 7.0 Hz), 4.47 (2H, d, J = 11 Hz), 6.64 (2H, dd, J = 8.2, 25 Hz),
6.84 (IH, m), 6.94 (IH, m), 7.03 (IH, s), 7.28 (IH, m), 7.56 (IH, br), 7.94 (IH, br),
9.46 (IH, s), 10.2 (IH, br), 13.5 (IH, br).
Examples 8-04 to 8-05
In the similar manners as described in Example 8-01 to 8-03 above, the compounds in Examples 8-04 to 8-05 as shown in Table 8 were synthesized.
Table 8
Example 9
N-{4-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-2-[2-(l-piperidinyl)- ethoxy]phenyl}acetamide ditrifluoroacetate
A mixture of 2-[(4-methoxybenzyl)oxy]acetophenone, 3-hydroxy-4-nitrobenzalde- hyde, malononitrile, ammonium acetate and toluene was stirred under reflux for 3 hrs. The usual work-up and column chromatography gave 2-amino-4-(3-hydroxy- 4-nitrophenyl)-6-{2-[(4-methoxybenzyl)oxy]-phenyl}nicotinonitrile.
A mixture of 2-amino-4-(3-hydroxy-4-nitrophenyl)-6-{2-[(4-methoxybenzyl)oxy]- phenyl}nicotinonitrile, cesium carbonate, potassium iodide and DMF was stirred at 80°C overnight. The usual work-up and column chromatography gave 2-amino-6- {2-[(4-methoxybenzyl)oxy]phenyl} -4- {4-nitro-3-[2-(l -piperidinyl)ethoxy]- phenyl} nicotinonitrile.
A mixture of 2-amino-6-{2-[(4-methoxybenzyl)-oxy]phenyl}-4-{4-nitro-3-[2-(l- piperidinyl)ethoxy]phenyl} -nicotinonitrile, SnCl2 and DMF was stirred at room temperature overnight. The usual work-up and column chromatography gave 2- amino-4-{4-amino-3-[2-(l-piperidinyl)ethoxy]phenyl}-6-{2-[(4-methoxybenzyl)- oxy]-phenyl}nicotinonitrile. A mixture of 2-amino-4-{4-amino-3-[2-(l-piperidinyl)ethoxy]phenyl}-6-{2-[(4- methoxybenzyl)oxy]-phenyl}nicotinonitrile, acetyl chloride, pyridine and CH C12 was stirred at 0°C for 1 hr. and at room temperature for 2 hrs. The usual work-up and column chromatography gave N-{4-(2-amino-3-cyano-6-{2-[(4-methoxybenzyl)- oxy]phenyl}-4-pyridinyl)-2-[2-(l-piperidinyl)ethoxy]-phenyl} acetamide.
A mixture of N-{4-(2-amino-3-cyano-6-{2-[(4-methoxybenzyl)oxy]phenyl}-4- pyridinyl)-2-[2-(l-piperidinyl)ethoxy]phenyl} acetamide and trifluoroacetic acid was stirred at room temperature for 3 hrs. Evaporation of trifluoroacetic acid gave N-{4- [2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-2-[2-(l -piperidinyl)ethoxy]- phenyl} acetamide ditrifluoroacetate.
Molecular weight: 699.612
Mass spectrometry: 472
In vitro activity grade: B Cellular activity grade: (A954)-B
Comparative examples
6-(2-Hydroxyphenyl)-4-(R"-phenyl)-3-cyano-2-aminopyridines (wherein R" is nitro or dimethylamino) disclosed in Mannna et al, (Eur. J. Med. Chem. 34 (1999) 245-
254) were synthesized according to the methods disclosed in the document. The effects of the synthesized compounds were examined by the in vitro assay method and cellular assay method disclosed herein. These compounds did not show any IKK-beta inhibitory activity and cytokine inhibitory activity.

Claims

A 4-aryl pyridine derivative of the formula (I):
wherein
X is CH orN;
R1 is hydrogen, hydroxy, halogen, d_6 alkyl, d_6 alkoxy, d.6 alkoxycarbonyl, nitro, amino, mono or di (d.6 alkyl)amino, phenylsulfonyl- amino, -NHR11 or -O-(CH2)n-R12 ,
wherein
R 11 is phenyl substituted d.6 alkyl, d-6 alkanoyl or .^alkylsulfonyl,
n represents an integer selected from 0 to 6, and
R 12 is C2.6 alkenyl, benzoyl, mono or di phenyl, mono or di (d_6 alkyl)amino, d-6 alkanoyl, d_6 alkoxycarbonyl, or a 3 to 10 membered saturated or unsaturated ring having 0 to 3 heteroatoms selected from the group consisting of S, O and N, and said ring is optionally substituted by hydroxy, nitro, cyano, mono or di halogen, Cι_6 alkyl, halogen substituted d-6 alkyl, amino, mono or di (d-6 alkyl)amino, d-e alkanoylamino, carbamoyl, Cι-6 alkoxy, C e alkoxycarbonyl, or phenyl;
R is hydrogen, hydroxy, halogen, or d-6 alkyl;
R3 is hydrogen, hydroxy, halogen, d_6 alkoxy, C3.6 cycloalkyl substituted d-6alkyloxy, -N R31R32, or a 3 to 6 membered saturated ring having 0 to 3 heteroatoms selected from the group consisting of O and N, and said ring is optionally substituted by R ;
Wherein
R >31 is hydrogen or Cι_6 alkyl
R32 is hydrogen, d-6 alkanoyl, or d.6 alkyl optionally substituted by hydroxy or phenyl,
R33 is nitro, cyano, d_6 alkyl optionally substituted by hydroxy or amino, d-6 alkoxy, hydroxy substituted d-6 alkyloxy, amino substituted Cι-6 alkyloxy, d-6 alkanoyl, carbamoyl or
-NR33aR33
Wherein
R ,33a is hydrogen or C].6 alkyl
R33 is hydrogen, d-6 alkyl optionally substituted by hydroxy or phenyl, d_6 alkanoyl, d-6 alkylsulfonyl, or tri- fluoroacetyl, is hydrogen, hydroxy, carboxy, -CO-NHR41, amino, d_6 alkylsulfonylamino, or -NH-COR41, d-6 alkyl optionally substituted by R42, d-e alkoxy, R43d-6 alkyloxy,
wherein
R41 is d-6 alkyl optionally substituted by R41a, d-6 alkoxy, oxo- tetrahydrofuryl, oxopyrrolidinyl, -CH(OH)R41 , -CH(NH2)R41c, -NHR41c, or piperazino optionally substituted by R41d,
wherein
R41a is carboxy, d-6 alkoxy, -CH(NH2)carboxy, -NR41a"!R41a"2, or a 3 to 10 membered saturated ring having 0 to 3 heteroatoms selected from the group consisting of O and N, and said ring is optionally substituted by carboxy, d-6 alkyl optionally substituted by hydroxy or benzodioxane, C3-6 cycloalkyl, d_6 alkanoyl, carboxy, benzyl, d_6 alkoxycarbonyl, or furoyl,
wherein
R41a_1 is hydrogen or d-6 alkyl optionally substituted by hydroxy, d-6 alkyloxy, C3.8 cycloalkyl, or piperidino,
R41a"2 is hydrogen or d-6 alkyl optionally substituted by hydroxy, d_6 alkyloxy or C3.6 cycloalkyl, d-6 alkoxy or a 3 to 6 membered saturated ring having 0 to 3 heteroatoms selected from the group consisting of O and N, and said ring is optionally substituted by carboxy, d.6 alkyl, d-6 alkanoyl, or d-6 alkyloxy,
R41b is d-6 alkyl optionally substituted by carboxy or d-6 alkyloxy, d.6 alkoxy, or d-6 alkoxycarbonyl,
R41c is carboxy, d_6 alkyl optionally substituted by carboxy or a 3 to 6 membered saturated ring having 0 to 3 heteroatoms selected from the group consisting of O and N, or
R41d is d-6 alkyl optionally substituted by carboxy or d.6 alkyloxy, or d-6 alkoxy
R ,42 is d-6 alkoxy, carboxy, amino, -CH(NH2)-carboxy or a 5 to 7 membered saturated or unsaturated ring having 0 to 3 heteroatoms selected from the group consisting of O and N, and said ring is optionally substituted by hydroxy, nitro, mono or di halogen, d_6 alkyl, halogen substituted Cι-6 alkyl, amino, mono or di (d-6 alkyl)amino,or carbamoyl;
R43 is carboxy, amino, -CH(NH2)-carboxy or a 5 to 7 membered saturated or unsaturated ring having 0 to 3 heteroatoms selected from the group consisting of O and N, and said ring is optionally substituted by hydroxy, nitro, mono or di halogen, Cι-6 alkyl, halogen substituted Cι.6 alkyl, amino, mono or di (Cι_6 alkyl)amino, or carbamoyl; R3 and R4 may form, together with the carbon atoms in the benzene ring, a 4 to 6 membered saturated ring having 0 to 3 heteroatoms selected from the group consisting of O and N, and said ring is optionally substituted by one or more substituents selected from the group consisting of hydroxy, nitro, mono or di halogen, d-6 alkyl, halogen substituted Cι-6 alkyl, oxo, amino, mono or di (Cι-6 alkyl)amino, and carbamoyl;
R5 is hydrogen, cyano, carboxy, carbamoyl, Ci-β alkyl optionally substituted by hydroxy or carbamoyl, and Cι-6 alkoxycarbonyl, and
R6 is -NR61R62 ,
wherein
R61 is hydrogen or Cι.6 alkyl,
R62 is hydrogen, Cι-6 alkyl, phenyl, benzyl, Cι_6 alkanoyl, or
NR61 R62 may form, together with the nitrogen atom to which they are attached, a saturated 5-6 membered ring optionally containing
NH or O as other heteroatoms than the adjacent N atom
or
R and R6 may form, together with the carbon atoms in the pyridine ring, a 5 to 7 membered saturated or unsaturated ring having 0 to 3 heteroatoms selected from the group consisting of O, S and N, and said ring optionally having substituents selected from the group consisting of halogen, nitro, cyano, oxo, thioxo, d_6 alkyl, d-6 alkylsulfonyl, d-6 alkoxy, d-6 alkoxycarbonyl, phenyl, d-6 alkanoyl, amino, Cι_6 alkyl- amino, C].6 alkanoylamino, carbamoyl, C3.8 cycloalkylaminocarbonyl, d-6 alkylaminocarbonyl, halogen substituted d.6 alkylaminocarbonyl, di (d-6 alkylaminocarbonyl, benzoylamino, phenyl- sulfonyl, di(d_6 alkyl)aminoCι-6 alkylaminocarbonyl, hydroindenyl- aminocarbonyl, diphenylmethylaminocarbonyl, pyrrolidinocarbonyl, d-6 alkyloxy d-6 alkyl amino carbonyl, morpholinocarbonyl, piperazinocarbonyl, phenyld-6alkylaminocarbonyl, carboxyCi-β alkylaminocarbonyl, C3.8 cycloalkylCi-6alkylaminocarbonyl, hydroxy Ci-βalkylaminocarbonyl, and methylsulfonylaminocarbonyl
or a salt thereof.
2. The compound or a salt thereof as claimed in claim 1 ,
wherein
X is CH or N;
R1 is hydrogen, halogen, Cι_6 alkyl, d-6 alkoxy or C3-6 cycloalkyl substituted d-6 alkyloxy;
R2 is hydrogen or halogen;
R3 is hydrogen, hydroxy, halogen, d-6 alkoxy, C3.6 cycloalkyl substituted d-βalkyloxy, amino, mono or di (d-6 alkyl)amino, Cι-6 alkanoyl- amino, d-6 alkyl(hydroxy substituted Cι_6 alkyl)amino, Ci-β alkyl-
(benzyl)amino, morpholino, piperidino, or pyrrolidino optionally substituted by d-6 alkyl, Cι-6 alkyloxy or hydroxy substituted Cj-6 alkyl, amino, di(Cι_6 alkyl)amino, or trifluoroacetylamino;
R4 is hydrogen, hydroxy, carboxy, Cι.6 alkyl optionally substituted by piperazino, Cι-6 alkoxy, piperidino substituted Cι_6 alkyloxy, amino, Cι-6 alkylsulfonylamino, piperidino Ci-6 alkyl- aminocarbonyl, or -NH-COR41,
wherein
R41 is Ci-6 alkyl optionally substituted by R41a, Ci-6 alkoxy, Cι_6 alkyloxy substituted Ci-6 alkyl, oxotefrahydrofuryl, oxopyr- rolidinyl, -CH(OH)R41 , -CH(NH2)R41c, or -NHR41c
wherein
R41a is Ci-e alkoxy, carboxy, -NHR413"1 (wherein R413"1 is hydrogen, or Cι-6 alkyl optionally substituted by hydroxy, Ci-6 alkyloxy, C3.8 cycloalkyl, or piperidino), -CH(NH2) -N(d-6 alkyl)R41a"2 (wherein R41a"2 is d-6 alkyl or Ci-β alkyl substituted pyrrolidinyl), pyrrolidinyl, piperidino optionally substituted by carboxy or d_6 alkoxycarbonyl, piperidino optionally fused with C .6 cycloalkyl, or piperazino optionally substituted by d_6 alkyl, hydroxy substituted Cι-6 alkyl, benzodioxane substituted d_6 alkyl, d.6 alkanoyl, C .6 cycloalkyl, benzyl, or furoyl,
R41 is Ci-6 alkyl optionally substituted by carboxy, or d_6 alkoxycarbonyl,
R4Ic is d-6 alkyl optionally substituted by carboxy or piperidino, R3 and R4 may form, together with the carbon atoms in the benzene ring, a 5 membered saturated ring having 0 to 2 heteroatoms selected from the group consisting of O and N, and said ring is optionally substituted by Ci-6 alkyl, or oxo;
R5 is hydrogen, carbamoyl, cyano, carboxy, Ci-6 alkoxycarbonyl, or hydroxy substituted Ci-6 alkyl; and
R6 is amino or Cι_6 alkanoylamino,
or
R5 and R6 may form, together with the carbon atoms in the pyridine ring, a 6 membered saturated or unsaturated ring having 0 to 3 heteroatoms selected from the group consisting of O, S and N, and said ring optionally having substituents selected from the group consisting of halogen, nitro, cyano, oxo, thioxo, Ci-6 alkyl, Cι-6 alkylsulfonyl, d-6 alkoxy, Cι-6 alkoxycarbonyl, phenyl, Cι_6 alkanoyl, amino, Cj.6 alkyl- amino, Cι-6 alkanoylamino, carbamoyl, C3.8 cycloalkylaminocarbonyl, Ci_6 alkylaminocarbonyl, halogen substituted Ci-6 alkylaminocarbonyl, and di (d_6 alkylaminocarbonyl
or a salt thereof.
The compound or a salt thereof as claimed in claim 1 ,
wherein
X is CH orN;
R1 is hydrogen, halogen, d.6 alkoxy, or cyclopropyl methoxy; R2 is hydrogen;
R is hydrogen, hydroxy, halogen, ammo, mono or di(d.6 alkyl)amino, acetamido, d-6alkoxy, cyclopropyl methoxy, Cι-6 alkyl(hydroxy substituted alkyl)amino, Cι.6 alkyl(benzyl)amino, morpholino, piperidino, or pyrrolidino optionally substituted by Cι_6 alkyl, hydroxy substituted Cι-6 alkyl, amino, di (Ci-6 alkyl)amino, or trifluoroacetyl- amino;
R4 is hydrogen, hydroxy, carboxy, amino, Ci-6 alkylsulfonylamino, piperazino, Ci-6 alkyl substituted piperazino, piperidino substituted Ci-6 alkyloxy, piperidino Cι.6 alkyl- aminocarbonyl, or -NH-COR41,
wherein
R41 is Ci-6 alkyl optionally substituted by R41a, Cι-6 alkoxy, oxotefrahydrofuryl, oxopyrrolidinyl, -CH(OH)R 1b, or -CH(NH2)R41c,
wherein
R41a is d-e alkoxy, carboxy, -CH(NH2)-carboxy, -NHR413"1 (wherein R41a_1 is hydrogen, Cι-6 alkyl optionally substituted by hydroxy, C3.8 cycloalkyl, Cι-6 alkoxy, or piperidino), -N(Cι.6 alkyl)R41a'2 (wherein R41a"2 is C,.6 alkyl or Cι-6 alkyl substituted pyrrolidinyl), pyrrolidinyl, piperidino optionally substituted by carboxy or Cj-6 alkoxycarbonyl, piperidino fused with cyclohexane, or piperazino optionally substituted by d-6 alkyl, hydroxy substituted Cι_6 alkyl, benzodioxane substituted Ci-6 alkyl, benzyl, Cι-6 alkanoyl, cyclohexyl, or furoyl,
R41 is d-6 alkyl optionally substituted by carboxy or Cι_6 alkoxycarbonyl,
R4Ic is Cι-6 alkyl optionally substituted by carboxy, or
R3 and R4 may form -NR401-CO-O-(wherein R401 is hydrogen or Cι-6 alkyl);
R5 is hydrogen, carbamoyl, cyano, carboxy, or hydroxymethyl; and
R6 is amino or acetamido
or
R5 and R6 may form -R50-CH2-NH-, -R50-CO-NH-, -R50-SO2-NH-, or -R50-C(=S)-NH-
wherein
R50 is -CHR501-O-, -CH2-N R501-, -CO-NR501-, (wherein R501 is hydrogen, Cι_6 alkyl, Cι_6 alkanoyl, d-6 alkoxycarbonyl, carbamoyl, Ci-6 alkylsulfonyl, C3.8 cycloalkylaminocarbonyl,
Ci-6 alkylaminocarbonyl, di (Cι_6 alkylaminocarbonyl or phenyl)
or a salt thereof. The compound as claimed in claim 1 selected from the group consisting of the following compounds:
5-({3-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl}amino)-4- hydroxy-5-oxopentanoate; N-{3-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl}-5-oxo- tefrahydro-2-furancarboxamide;
N-{3-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4- pyridinyl]phenyl}methanesulfonamide;
N4- {3-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl} -L- asparagine;
N1-{3-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl}-L- asparagine;
N- {3-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl} -3-(l - piperidinyl)propanamide; 5-({4-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl} amino)-4- hydroxy-5-oxopentanoate;
4-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-N-[2-(l-piperidinyl)- ethyl]benzamide;
4-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]benzoic acid; 5-({5-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-ρyridinyl]-2-chlorophenyl}- amino)-4-hydroxy-5-oxopentanoate;
N-{3-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl}-2-[4-(2- furoyl)- 1 -piperazinyl]acetamide;
N-{3-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl}-3-(4-ethyl- l-piperazinyl)propanamide;
N-{3-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl}-3-octa- hydro- 1 (2H)-quinolinylpropanamide;
N1 - {3-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl} -N2-cyclo- pentylglycinamide; N1 - {3-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl} -N2-
(cyclopropylmethyl)glycinamide; N1-{3-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl}-N2- propylglycinamide;
N1-{3-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl}-N2-(3-hy- droxypropyl)glycinamide; N1- {3-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl} -N3-
(cyclopropylmethyl)-β-alaninamide;
N1-{3-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl}-N2,N2- dimethylglycinamide;
N- {3 - [2-amino-3 -cyano-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl} -2-( 1 - pyrrolidinyl)acetamide;
N1 - {3 - [2-amino-3 -cyano-6-(2-hydroxyphenyl)-4-pyridinyl]phenyl} -N2- [2-
(methyloxy)ethyl] glycinamide;
N1-[5-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-2-(3-amino-l- pyrrolidinyl)phenyl]-β-alaninamide; N1 - [5 - [2-amino-3 -cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-2-( 1 -piperidinyl)- phenyl]-β-alaninamide;
N1 - [5- [2-amino-3 -cy ano-6-(2-hydroxyphenyl)-4-pyridinyl] -2-( 1 - pyrrolidinyl)phenyl]-β-alaninamide;
N1 - { 5 - [2-amino-3 -cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-2- [ethyl(2- hydroxyethyl)amino]phenyl} -β-alaninamide;
N1 -[5 - [2-amino-3 -cyano-6-(2-hydroxyphenyl)-4-pyridinyl] -2-(4- mo holinyl)phenyl]-β-alaninamide;
N1-[5-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-2-(dimethyl- amino)phenyl]-β-alaninamide; N- {3-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-4-chlorophenyl} -
3-( 1 -piperidinyl)propanamide;
2-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]benzoic acid;
2-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-N-[2-(l-piperidinyl)- ethyljbenzamide; N1 - {5-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-2-chlorophenyl} -
N -(cyclopropylmethyl)glycinamide; N1-{5-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-2-chlorophenyl}-
N2-(2-methoxyethyl)glycinamide;
N1-{5-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-2-chlorophenyl}- β-alaninamide; 2-amino-4-(4-amino-3 -hydroxyphenyl)-6-(2-hydroxyphenyl)nicotinonitrile;
N-{4-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-2-hydroxy- phenyl} acetamide;
N-{5-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-2-chlorophenyl}-
3 -( 1 -piperidinyl)propanamide; N1-[5-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-2-(dimetliyl- amino)phenyl]-N2-(cyclopropylmethyl)glycinamide;
N-[5-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-2-(dimethyl- amino)phenyl]-3-(l-piperidinyl)propanamide;
N1-{5-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-2-[(2S)-2- (hydroxymethyl)- 1 -pyrrolidinyl]phenyl} -L-leucinamide;
N1-{5-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-2-[(3S)-3-
(dimethylamino)- 1 -pyrrolidinyljphenyl} -N2-(cyclopropylmethyl)glycin- amide;
N1-(5-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-2-{3-[(trifluoro- acetyl)amino]-l-pyrrolidinyl}phenyl)-L-leucinamide;
N-(5-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-2-{3-[(trifluoro- acetyl)amino]-l-pyrrolidinyl}phenyl)-3-(l-piperidinyl)propanamide;
N1-(5-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-2-{3-[(trifluoro- acetyl)amino]- 1 -pyrrolidinyl}phenyl)-N -(2-methoxyethyl)glycinamide; N1-(5-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-2-{3-[(trifluoro- acetyl)amino]-l-pyrrolidinyl}phenyl)-N -(cyclopropylmethyl)glycinamide;
2-amino-6-(2-hydroxyphenyl)-4-(3-hydroxyphenyl)nicotinonitrile;
N-{4-[2-amino-3-cyano-6-(2-hydroxyphenyl)-4-pyridinyl]-2-[2-(l- piperidinyl)ethoxy]phenyl}acetamide; N-{4-[2-amino-3-(hydroxymethyl)-6-(2-hydroxyphenyl)-4-pyridinyl]-2- hydroxyphenyl} acetamide; 2-[6-amino-4-[4-(ethylamino)-3-hydroxyphenyl]-5-(hydroxymethyl)-2- pyridinyl]-3-(cyclopropylmethoxy)phenol;
N-{4-[2-amino-6-[2-(cyclopropyhnethoxy)-6-hydroxyphenyl]-3-(hydroxy- methyl)-4-pyridinyl]-2-hydroxyphenyl} acetamide;
7-[2-(cyclopropylmethoxy)-6-hydroxyphenyl]-5-(3-ethyl-2-oxo-2,3-dihydro- l,3-benzoxazol-6-yl)-l,4-dihydro-2H-pyrido[2,3-d][l,3]oxazin-2-one;
5. A pharmaceutical composition comprising the compound or a salt thereof as claimed in claim 1 as an active ingredient.
6. A pharmaceutical composition comprising the compound or a salt thereof as claimed in claim 1 together with one or more pharmaceutically acceptable excipients.
7. An IKB kinase β inhibitor comprising the compound or a salt thereof as claimed in claim 1 as an active ingredient.
8. An anti-inflammatory agent comprising the compound or a salt thereof as claimed in claim 1 as an active ingredient.
9. The anti-inflammatory agent as claimed in claim 6, wherein said agent is used to treat or prevent a disease selected from the group consisting of asthma; allergic rhinitis; atopic dermatitis; hives; conjunctivitis; vernal catarrh; chronic arthrorheumatism; systemic lupus erythematosus; psoriasis; diabrotic colitis; systemic inflammatory response syndrome (SIRS); sepsis; polymyositis; dermatomyositis (DM); polyarthritis nodosa (PN); mixed connective tissue disease (MCTD); Sjoegren's syndrome; and gout.
10. An immunosuppressant comprising the compound or a salt thereof as claimed in claim 1 as an active agent.
11. An agent to treat ischemia comprising the compound or a salt thereof as claimed in claim 1 as an active agent.
12. An anti-tumor agent comprising the compound or a salt thereof as claimed in claim 1 as an active agent.
13. Use of compounds of the general formula (I) according to any one of Claims 1 to 4 for the production of medicaments.
14. Use according to Claim 13, in which the medicaments are for the control of inflammatory disorders.
EP01991731A 2000-12-01 2001-11-19 4-6-diphenyl pyridine derivatives as antiinflammatory agents Expired - Lifetime EP1339687B1 (en)

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Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2305125T3 (en) 2000-10-26 2008-11-01 Amgen Inc. ANTI-INFLAMMATORY AGENTS.
AR037641A1 (en) 2001-12-05 2004-11-17 Tularik Inc INFLAMMATION MODULATORS
DE10229762A1 (en) 2002-07-03 2004-01-22 Aventis Pharma Deutschland Gmbh Pyrazoloisoquinoline derivatives for the inhibition of NFkappaB-inducing kinase
WO2004041285A1 (en) 2002-10-31 2004-05-21 Amgen Inc. Antiinflammation agents
DE10259933A1 (en) * 2002-12-20 2004-07-01 Bayer Healthcare Ag Substituted 4-aryl pyridines
US7132428B2 (en) 2003-07-03 2006-11-07 Aventis Pharmaceuticals Inc. Pyrazoloisoquinoline derivative as kinase inhibitors for the treatment of various disorders
CA2541667A1 (en) * 2003-10-14 2005-04-21 Pharmacia Corporation Substituted pyrazinone compounds for the treatment of inflammation
US20070265289A1 (en) * 2004-04-15 2007-11-15 Yoshinori Okamoto 2-Aminopyrimidine Derivative
AR050253A1 (en) 2004-06-24 2006-10-11 Smithkline Beecham Corp COMPOSITE DERIVED FROM INDAZOL CARBOXAMIDE, COMPOSITION THAT INCLUDES IT AND ITS USE FOR THE PREPARATION OF A MEDICINAL PRODUCT
PE20060748A1 (en) 2004-09-21 2006-10-01 Smithkline Beecham Corp INDOLCARBOXAMIDE DERIVATIVES AS KINASE INHIBITORS IKK2
US20090018145A1 (en) * 2004-10-19 2009-01-15 Compass Pharmaceuticals Llc Compositions and their use as anti-tumor agents
US8063071B2 (en) 2007-10-31 2011-11-22 GlaxoSmithKline, LLC Chemical compounds
AR055343A1 (en) 2005-06-30 2007-08-22 Smithkline Beecham Corp INDOLCARBOXAMIDE INHIBITORS DERIVATIVES OF QUINASAS IKK2
AU2006275403A1 (en) * 2005-08-02 2007-02-08 Lexicon Pharmaceuticals, Inc. 2-aminoaryl pyridines as protein kinases inhibitors
AU2006287767B2 (en) 2005-09-07 2010-05-13 Merck Serono Sa IKK inhibitors for the treatment of endometriosis
EP2001455A2 (en) 2006-03-15 2008-12-17 Theralogics, Inc. Methods of treating muscular wasting diseases using nf-kb activation inhibitors
PE20081889A1 (en) 2007-03-23 2009-03-05 Smithkline Beecham Corp INDOL CARBOXAMIDES AS INHIBITORS OF IKK2
US11969501B2 (en) 2008-04-21 2024-04-30 Dompé Farmaceutici S.P.A. Auris formulations for treating otic diseases and conditions
EP2278999B1 (en) 2008-04-21 2025-01-29 Dompé farmaceutici S.p.A. Auris formulations for treating otic diseases and conditions
EP2366699B1 (en) 2008-10-02 2013-08-07 Asahi Kasei Pharma Corporation 8-substituted isoquinoline derivative and use thereof
WO2010102968A1 (en) 2009-03-10 2010-09-16 Glaxo Group Limited Indole derivatives as ikk2 inhibitors
US8629164B2 (en) * 2009-03-27 2014-01-14 Profectus Biosciences, Inc. Inhibitors of NF-κB
EP2440204B1 (en) 2009-06-12 2013-12-18 Bristol-Myers Squibb Company Nicotinamide compounds useful as kinase modulators
CN102321014A (en) * 2011-05-27 2012-01-18 沈阳亿灵医药科技有限公司 Novel di-phenylpyridine derivative
CA2988421A1 (en) * 2015-06-01 2016-12-08 The Scripps Research Institute Small molecule analogs of the nemo binding peptide
JP2019507179A (en) 2016-03-01 2019-03-14 プロペロン セラピューティックス インコーポレイテッド Inhibitors of WDR5 protein-protein binding
US11319299B2 (en) 2016-03-01 2022-05-03 Propellon Therapeutics Inc. Substituted carboxamides as inhibitors of WDR5 protein-protein binding
AU2017290256A1 (en) 2016-06-29 2019-01-17 Otonomy, Inc. Triglyceride otic formulations and uses thereof
CN109020883B (en) * 2018-03-27 2022-02-08 广西民族大学 A kind of 3-cyano-2-aminopyridine derivative and its preparation method and application

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4272338B2 (en) * 2000-09-22 2009-06-03 バイエル アクチェンゲゼルシャフト Pyridine derivatives

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO0244153A1 *

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JP2004514712A (en) 2004-05-20
US20040097563A1 (en) 2004-05-20
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WO2002044153A8 (en) 2002-11-28
AU2002231628A1 (en) 2002-06-11
US7410986B2 (en) 2008-08-12
ES2298288T3 (en) 2008-05-16
EP1339687B1 (en) 2008-01-23
DE60132618D1 (en) 2008-03-13
CA2430354A1 (en) 2002-06-06
DE60132618T2 (en) 2009-02-19

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