EP1312602A1 - Amidino derivatives, and anticoagulants and thrombosis therapeutic agents containing them - Google Patents

Amidino derivatives, and anticoagulants and thrombosis therapeutic agents containing them Download PDF

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EP1312602A1
EP1312602A1 EP02025580A EP02025580A EP1312602A1 EP 1312602 A1 EP1312602 A1 EP 1312602A1 EP 02025580 A EP02025580 A EP 02025580A EP 02025580 A EP02025580 A EP 02025580A EP 1312602 A1 EP1312602 A1 EP 1312602A1
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Prior art keywords
amidino
pharmacologically acceptable
solvate
acceptable salt
naphthyloxy
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German (de)
French (fr)
Inventor
Takashi Satoh
Yasushi Okamoto
Osamu Asano
Nobuhisa Watanabe
Tadashi Nagakura
Takao Saeki
Atsushi Inoue
Masahiro Sakurai
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Eisai R&D Management Co Ltd
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Eisai Co Ltd
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    • C07D333/50—Heterocyclic compounds containing five-membered rings having one sulfur atom as the only ring hetero atom condensed with carbocyclic rings or ring systems
    • C07D333/52—Benzo[b]thiophenes; Hydrogenated benzo[b]thiophenes
    • C07D333/62—Benzo[b]thiophenes; Hydrogenated benzo[b]thiophenes with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to carbon atoms of the hetero ring
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
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    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C257/00—Compounds containing carboxyl groups, the doubly-bound oxygen atom of a carboxyl group being replaced by a doubly-bound nitrogen atom, this nitrogen atom not being further bound to an oxygen atom, e.g. imino-ethers, amidines
    • C07C257/10—Compounds containing carboxyl groups, the doubly-bound oxygen atom of a carboxyl group being replaced by a doubly-bound nitrogen atom, this nitrogen atom not being further bound to an oxygen atom, e.g. imino-ethers, amidines with replacement of the other oxygen atom of the carboxyl group by nitrogen atoms, e.g. amidines
    • C07C257/18—Compounds containing carboxyl groups, the doubly-bound oxygen atom of a carboxyl group being replaced by a doubly-bound nitrogen atom, this nitrogen atom not being further bound to an oxygen atom, e.g. imino-ethers, amidines with replacement of the other oxygen atom of the carboxyl group by nitrogen atoms, e.g. amidines having carbon atoms of amidino groups bound to carbon atoms of six-membered aromatic rings
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    • C07C275/28—Derivatives of urea, i.e. compounds containing any of the groups, the nitrogen atoms not being part of nitro or nitroso groups having nitrogen atoms of urea groups bound to carbon atoms of six-membered aromatic rings of a carbon skeleton
    • C07C275/42—Derivatives of urea, i.e. compounds containing any of the groups, the nitrogen atoms not being part of nitro or nitroso groups having nitrogen atoms of urea groups bound to carbon atoms of six-membered aromatic rings of a carbon skeleton being further substituted by carboxyl groups
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    • C07C311/00—Amides of sulfonic acids, i.e. compounds having singly-bound oxygen atoms of sulfo groups replaced by nitrogen atoms, not being part of nitro or nitroso groups
    • C07C311/01—Sulfonamides having sulfur atoms of sulfonamide groups bound to acyclic carbon atoms
    • C07C311/02—Sulfonamides having sulfur atoms of sulfonamide groups bound to acyclic carbon atoms of an acyclic saturated carbon skeleton
    • C07C311/08—Sulfonamides having sulfur atoms of sulfonamide groups bound to acyclic carbon atoms of an acyclic saturated carbon skeleton having the nitrogen atom of at least one of the sulfonamide groups bound to a carbon atom of a six-membered aromatic ring
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    • C07C311/00—Amides of sulfonic acids, i.e. compounds having singly-bound oxygen atoms of sulfo groups replaced by nitrogen atoms, not being part of nitro or nitroso groups
    • C07C311/15—Sulfonamides having sulfur atoms of sulfonamide groups bound to carbon atoms of six-membered aromatic rings
    • C07C311/21—Sulfonamides having sulfur atoms of sulfonamide groups bound to carbon atoms of six-membered aromatic rings having the nitrogen atom of at least one of the sulfonamide groups bound to a carbon atom of a six-membered aromatic ring
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    • C07C311/22—Sulfonamides, the carbon skeleton of the acid part being further substituted by singly-bound oxygen atoms
    • C07C311/29—Sulfonamides, the carbon skeleton of the acid part being further substituted by singly-bound oxygen atoms having the sulfur atom of at least one of the sulfonamide groups bound to a carbon atom of a six-membered aromatic ring
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    • C07C311/00—Amides of sulfonic acids, i.e. compounds having singly-bound oxygen atoms of sulfo groups replaced by nitrogen atoms, not being part of nitro or nitroso groups
    • C07C311/30—Sulfonamides, the carbon skeleton of the acid part being further substituted by singly-bound nitrogen atoms, not being part of nitro or nitroso groups
    • C07C311/45—Sulfonamides, the carbon skeleton of the acid part being further substituted by singly-bound nitrogen atoms, not being part of nitro or nitroso groups at least one of the singly-bound nitrogen atoms being part of any of the groups, X being a hetero atom, Y being any atom, e.g. N-acylaminosulfonamides
    • C07C311/46—Y being a hydrogen or a carbon atom
    • C—CHEMISTRY; METALLURGY
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    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C317/00—Sulfones; Sulfoxides
    • C07C317/14—Sulfones; Sulfoxides having sulfone or sulfoxide groups bound to carbon atoms of six-membered aromatic rings
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    • C07D217/00—Heterocyclic compounds containing isoquinoline or hydrogenated isoquinoline ring systems
    • C07D217/22—Heterocyclic compounds containing isoquinoline or hydrogenated isoquinoline ring systems with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to carbon atoms of the nitrogen-containing ring
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    • C07D233/00—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings
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    • C07D233/66—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings having two double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D233/84—Sulfur atoms
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    • C07D333/00—Heterocyclic compounds containing five-membered rings having one sulfur atom as the only ring hetero atom
    • C07D333/02—Heterocyclic compounds containing five-membered rings having one sulfur atom as the only ring hetero atom not condensed with other rings
    • C07D333/04—Heterocyclic compounds containing five-membered rings having one sulfur atom as the only ring hetero atom not condensed with other rings not substituted on the ring sulphur atom
    • C07D333/06—Heterocyclic compounds containing five-membered rings having one sulfur atom as the only ring hetero atom not condensed with other rings not substituted on the ring sulphur atom with only hydrogen atoms, hydrocarbon or substituted hydrocarbon radicals, directly attached to the ring carbon atoms
    • C07D333/22—Radicals substituted by doubly bound hetero atoms, or by two hetero atoms other than halogen singly bound to the same carbon atom
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    • C07—ORGANIC CHEMISTRY
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    • C07D333/00—Heterocyclic compounds containing five-membered rings having one sulfur atom as the only ring hetero atom
    • C07D333/02—Heterocyclic compounds containing five-membered rings having one sulfur atom as the only ring hetero atom not condensed with other rings
    • C07D333/04—Heterocyclic compounds containing five-membered rings having one sulfur atom as the only ring hetero atom not condensed with other rings not substituted on the ring sulphur atom
    • C07D333/26—Heterocyclic compounds containing five-membered rings having one sulfur atom as the only ring hetero atom not condensed with other rings not substituted on the ring sulphur atom with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D333/28—Halogen atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D333/00—Heterocyclic compounds containing five-membered rings having one sulfur atom as the only ring hetero atom
    • C07D333/02—Heterocyclic compounds containing five-membered rings having one sulfur atom as the only ring hetero atom not condensed with other rings
    • C07D333/04—Heterocyclic compounds containing five-membered rings having one sulfur atom as the only ring hetero atom not condensed with other rings not substituted on the ring sulphur atom
    • C07D333/26—Heterocyclic compounds containing five-membered rings having one sulfur atom as the only ring hetero atom not condensed with other rings not substituted on the ring sulphur atom with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D333/30—Hetero atoms other than halogen
    • C07D333/34—Sulfur atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D333/00—Heterocyclic compounds containing five-membered rings having one sulfur atom as the only ring hetero atom
    • C07D333/02—Heterocyclic compounds containing five-membered rings having one sulfur atom as the only ring hetero atom not condensed with other rings
    • C07D333/04—Heterocyclic compounds containing five-membered rings having one sulfur atom as the only ring hetero atom not condensed with other rings not substituted on the ring sulphur atom
    • C07D333/26—Heterocyclic compounds containing five-membered rings having one sulfur atom as the only ring hetero atom not condensed with other rings not substituted on the ring sulphur atom with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D333/38—Carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D333/00—Heterocyclic compounds containing five-membered rings having one sulfur atom as the only ring hetero atom
    • C07D333/02—Heterocyclic compounds containing five-membered rings having one sulfur atom as the only ring hetero atom not condensed with other rings
    • C07D333/04—Heterocyclic compounds containing five-membered rings having one sulfur atom as the only ring hetero atom not condensed with other rings not substituted on the ring sulphur atom
    • C07D333/26—Heterocyclic compounds containing five-membered rings having one sulfur atom as the only ring hetero atom not condensed with other rings not substituted on the ring sulphur atom with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D333/38—Carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals
    • C07D333/40—Thiophene-2-carboxylic acid

Definitions

  • the present invention relates to medicinally useful novel amidino derivatives, to their pharmacologically acceptable salts or solvates, and to blood clotting factor VIIa inhibitors, anticoagulants and thrombosis therapeutic agents containing them as active ingredients.
  • thrombosis Destruction of blood vessels in the body triggers rapid production of thrombin to prevent death by bleeding.
  • overproduction of thrombin accompanying inflammation reaction at damaged blood vessels leads to thrombosis, which impairs the functioning of important organs.
  • Thrombin inhibitors such as heparin and warfarin, which inhibit thrombin production or directly inhibit thrombin activity, have long been used as anticoagulants for treatment or prevention of thrombosis.
  • heparin and warfarin which inhibit thrombin production or directly inhibit thrombin activity
  • blood clotting factor VIIa inhibitors unlike thrombin inhibitors, are expected to produce milder hemorrhage since the intrinsic coagulation pathway still remains, and are thus believed to be useful from this viewpoint as well.
  • Such compounds that have been conventionally known to inhibit blood clotting factor VIIa include amidinonaphthol derivatives (Tetrahedron, Vol.55, p.6219, 1999), amidino derivatives (International Publication No. WO99/41231) N-sulfonyl dipeptide derivatives (International Publication No. WO00/58346), 6-[[(allyl)oxy]methyl]naphthalene-2-carboximidamide derivatives (International Publication No. WO00/66545), and the like.
  • the present invention provides:
  • halogen atoms C 1-6 alkyl, C 1-6 alkoxy, C 1-6 alkylsulfonyl, C 3-8 cycloalkyl, C 3-8 cycloalkoxy, halo C 1-6 alkyl, halo C 1-6 alkoxy, C 6-14 aryl, C 1-6 alkoxy-C 6-14 aryl, C 6-14 aryloxy, C 6-14 arylsulfonyl, nitro, C 1-6 alkoxycarbonyl, carboxyl, acetamide, hydroxyl, 5- to 14-membered aromatic heterocyclic groups, CF 3 -, CF 3 O-, Z-CO- ⁇ where Z represents -NR 7 R 8 (wherein R 7 and R 8 may be the same or different and each represents hydrogen atom, C 1-6 alkyl, C 3-8 cycloalkyl, C 6-14 aryl, benzyl or C 3-8 cycloalkylmethyl), 1-pyrrolidinyl
  • halogen atoms C 1-6 alkyl, C 1-6 alkoxy, C 1-6 alkylsulfonyl, C 3-8 cycloalkyl, C 3-8 cycloalkoxy, halo C 1-6 alkyl, halo C 1-6 alkoxy, C 6-14 aryl, C 1-6 alkoxy-C 6-14 aryl, C 6-14 aryloxy, C 6-14 arylsulfonyl, nitro, C 1-6 alkoxycarbonyl, carboxyl, acetamide, hydroxyl, 5- to 14-membered aromatic heterocyclic groups, CF 3 -, CF 3 O-, Z-CO- ⁇ where Z represents -NR 7 R 8 (wherein R 7 and R 8 may be the same or different and each represents hydrogen atom, C 1-6 alkyl, C 3-8 cycloalkyl, C 6-14 aryl, benzyl or C 3-8 cycloalkylmethyl), 1-pyrrolidinyl
  • amidino derivatives of the invention are represented by general formula (I) given above.
  • amidino derivatives represented by the following general formula (II): (where X, Y, Ar 1 and R 3 have the same definitions as X, Y, Ar 1 and R 3 for general formula (I)), or pharmacologically acceptable salts or solvates thereof.
  • the group represented by Ar 1 in general formula (I) is 2,6-naphthylene, 1,4-phenylene, 1,3-phenylene, a group represented by the following formula: or a group represented by the following formula: and for more enhanced pharmacological activity it is preferably 2,6-naphthylene.
  • X in general formula (I) is C 1-6 alkyl, a halogen atom, -NH 2 or a group represented by the following formula (IV): ⁇ where R 1 represents optionally substituted C 1-6 alkyl, optionally substituted C 1-6 alkoxy, optionally substituted C 3-8 cycloalkyl, optionally substituted C 6-14 aryl, optionally substituted C 7-20 aralkyl, an optionally substituted 5- to 14-membered aromatic heterocyclic group or a group represented by the following formula (V): (where R 4 represents hydrogen atom, C 1-6 alkyl or optionally substituted C 2-7 acyl), R 2 represents hydrogen atom or C 1-6 alkyl, and X 2 represents -CO-, -SO 2 -, -NH-CO- or a single bond ⁇ , but for more enhanced pharmacological activity it is preferably C 1-6 alkyl or a group represented by the aforementioned formula (IV) (for example, R 1 -NHCONR 2
  • the group represented by X 2 in general formula (IV) is -CO-, -SO 2 -, -NH-CO- or a single bond, but for more enhanced pharmacological activity it is preferably -CO-, -SO 2 - or -NH-CO-, and most preferably -SO 2 -.
  • the group represented by R 1 in general formula (IV) is (i) optionally substituted C 1-6 alkyl, (ii) optionally substituted C 1-6 alkoxy, (iii) optionally substituted C 3-8 cycloalkyl, (iv) optionally substituted C 6-14 aryl, (v) optionally substituted C 7-20 aralkyl, (vi) an optionally substituted 5- to 14-membered aromatic heterocyclic group or (vii) a group represented by formula (V) above, and it is preferably (i), (iii), (iv), (v), (vi) or (vii); for more enhanced pharmacological activity it is most preferably (iv) or (vii).
  • R 4 in formula (V) above is hydrogen atom, C 1-6 alkyl or optionally substituted C 2-7 acyl, but for more enhanced pharmacological activity it is preferably optionally substituted C 2-7 acyl.
  • R 2 in formula (IV) above is hydrogen atom or C 1-6 alkyl, but for more enhanced pharmacological activity it is preferably hydrogen atom.
  • R 3 in general formula (I) is hydrogen atom, hydroxyl, C 2-7 acyl or C 2-7 alkoxycarbonyl, but for more enhanced pharmacological activity it is preferably hydrogen atom.
  • the group represented by Y in general formula (I) is a group represented by the formula -Ar 2 -COOR 5 (where Ar 2 represents optionally substituted C 6-14 aryl, an optionally substituted 5- to 14-membered aromatic heterocyclic group or a single bond, and R 5 represents hydrogen atom or C 1-6 alkyl).
  • the group represented by Y is preferably a carboxyl group (-COOH) or a group represented by the formula -Ar 2 -COOH (where Ar 2 represents optionally substituted C 6-14 aryl), more preferably carboxyl or optionally substituted carboxyphenyl, and most preferably a group represented by the following formula:
  • C 1-6 alkyl used throughout the present specification refers to a linear or branched alkyl group of 1-6 carbons (preferably 1-4 carbons), such as methyl, ethyl, n-propyl, i-propyl, n-butyl, s-butyl, t-butyl, pentyl, isopentyl, hexyl or the like.
  • C 1-6 alkoxy refers to a linear or branched alkoxy group of 1-6 carbons (preferably 1-4 carbons), such as methoxy, ethoxy, n-propoxy, i-propoxy, n-butoxy, s-butoxy, t-butoxy, pentoxy, isopentoxy, hexoxy or the like.
  • C 3-8 cycloalkyl refers to a cyclic saturated hydrocarbon group of 3-8 carbons (preferably 3-6 carbons) such as cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl or the like.
  • C 6-14 aryl refers to a monovalent aromatic hydrocarbon group of 6-14 carbons (preferably 6-10 carbons) lacking one of its hydrogen atoms, such as phenyl, biphenyl, naphthyl, anthranyl, phenanthryl, tolyl, xylyl or the like, with phenyl and naphthyl being preferred for more enhanced pharmacological activity.
  • the C 6-14 aryl group referred to here need not necessarily be monovalent, and it may be a divalent group lacking yet another hydrogen atom.
  • C 7-20 aralkyl refers to a group which is an alkyl group substituted with an aryl group at one of its hydrogen atoms, and has 7-20 (preferably 7-14) carbons, such as benzyl, phenethyl or the like.
  • 5- to 14-membered aromatic heterocyclic group refers to a 5- to 14-membered aromatic heterocyclic group having at least one hetero atom (nitrogen atom, oxygen atom, sulfur atom, etc.), such as thienyl, pyridyl, imidazolyl, pyrazinyl, pyrimidyl or the like.
  • the 5- to 14-membered aromatic heterocyclic group referred to here need necessarily not be monovalent, and it may be a divalent group lacking yet another hydrogen atom.
  • C 2-7 acyl refers to a carbonyl group substituted with an alkyl group, aryl group, alkoxy group, etc. and having 2-7 carbons, and is preferably a carbonyl group bonded to a C 1-6 alkyl group.
  • Examples of such C 2-7 acyl groups include alkylcarbonyl groups such as acetyl, propionyl, butanoyl, pivaloyl and cyclohexanecarbonyl and arylcarbonyl groups such as benzoyl, among which acetyl, propionyl and butanoyl are preferred.
  • C 2-7 alkoxycarbonyl refers to a carbonyl group bonded to a C 1-6 alkoxy group as defined above and having 2-7 carbons, such as methoxycarbonyl, ethoxycarbonyl or the like.
  • halogen atom refers to a fluorine atom, chlorine atom, bromine atom or iodine atom.
  • substituents there may be mentioned hydrogen atom, halogen atoms (fluorine, chlorine, bromine and iodine), C 1-6 alkyl, C 1-6 alkoxy, C 1-6 alkylsulfonyl, C 3-8 cycloalkyl, C 3-8 cycloalkoxy, halo C 1-6 alkyl, halo C 1-6 alkoxy, C 6-14 aryl, C 1-6 alkoxy-C 6-14 aryl, C 6-14 aryloxy, C 6-14 arylsulfonyl, nitro, C 1-6 alkoxycarbonyl, carboxyl, acetamide, hydroxyl, 5- to 14-membered aromatic heterocyclic groups, CF 3 -, CF 3 O-, Z-CO- ⁇ where Z represents -NR 7 R 8 (wherein R 7 and R 8 may be
  • salts of inorganic acids salts of organic acids, salts of inorganic bases, salts of organic bases, salts of acidic or basic amino acids and the like.
  • salts of inorganic acids there may be mentioned salts of hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid and the like
  • salts of organic acids there may be mentioned salts of acetic acid, succinic acid, fumaric acid, maleic acid, tartaric acid, citric acid, lactic acid, stearic acid, benzoic acid, methanesulfonic acid, p-toluenesulfonic acid and the like.
  • salts of inorganic bases there may be mentioned alkali metal salts such as sodium salts, potassium salts and the like, alkaline earth metal salts such as calcium salts, magnesium salts and the like, and aluminum salts, ammonium salts, or the like.
  • salts of organic bases there may be mentioned salts of diethylamine, diethanolamine, meglumine, N,N'-dibenzylethylenediamine and the like.
  • salts of acidic amino acids there may be mentioned salts of aspartic acid, glutamic acid and the like, and as preferred examples of salts of basic amino acids there may be mentioned salts of arginine, lysine, ornithine and the like.
  • the aforementioned acids or bases are those that form salts at an appropriate ratio in a range of 0.1-5 molecules per molecule of the compound.
  • the pharmacologically acceptable salts of the amidino derivatives of the invention also include prodrugs, and for example, the aforementioned amidino derivative esters may be mentioned, with t-butyl esters being preferred.
  • amidino derivatives of the invention or their pharmacologically acceptable salts may be in the form of solvates, where such solvates are preferably hydrates.
  • amidino derivatives of the invention are novel compounds which may be synthesized in the following manner, as an example.
  • amidino derivatives of the invention represented by general formula (I) above may be synthesized utilizing ordinary organic chemical reactions which are already known, and for example, they may be synthesized by the process represented by the reaction scheme (A) shown below, or by a similar process.
  • reaction scheme (A) shown below or by a similar process.
  • the symbols of the compounds in the following schemes are the same as defined above.
  • Compound (1a) may be reacted by the same procedure described in Synthetic Communications, Vol.26 (23), p.4351, 1996 to obtain compound (3a). Specifically, compound (1a) and a hydroxyamine (2a) are reacted in a solvent (for example, methanol or ethanol) in the presence of a base (for example, potassium carbonate).
  • a solvent for example, methanol or ethanol
  • a base for example, potassium carbonate
  • Compound (3a) may be reacted by the same procedure described in Synthetic Communications, Vol.26 (23), p.4351, 1996 to obtain compound (4a). Specifically, compound (3a) and acetic anhydride are reacted in a solvent (for example, acetic acid), and then a palladium catalyst is added for reaction in hydrogen gas.
  • a solvent for example, acetic acid
  • Compound (4a) can also be produced by employing the Pinner method.
  • reaction is first conducted from -10°C to 50°C using hydrochloric acid in a solvent (for example, methanol, ethanol or methylene chloride), and then reaction is conducted from -10°C to 50°C using ammonia gas in a solvent (for example, methanol or ethanol).
  • a solvent for example, methanol, ethanol or methylene chloride
  • Compound (4a) may be hydrolyzed in an acid (for example, hydrochloric acid, trifluoroacetic acid or sulfuric acid) to obtain compound (5a). Specifically, compound (4a) is reacted in a solvent (for example, tetrahydrofuran) in the presence of the aforementioned acid.
  • an acid for example, hydrochloric acid, trifluoroacetic acid or sulfuric acid
  • a solvent for example, tetrahydrofuran
  • Compound (1a) may be hydrolyzed in an aqueous solution of a base (for example, sodium hydroxide or lithium hydroxide) to obtain compound (6a). Specifically, compound (1a) is reacted in a solvent (for example, a mixture of tetrahydrofuran and methanol) in the presence of the aforementioned base aqueous solution.
  • a base for example, sodium hydroxide or lithium hydroxide
  • a solvent for example, a mixture of tetrahydrofuran and methanol
  • Compound (6a) and compound (2a) may be used to obtain compound (7a) by the same method as Step 1A.
  • Compound (7a) may be used to obtain compound (5a) by the same method as Step 2A.
  • a library for compound (5a) may be constructed by combination of substituent types and positions in compound (5a).
  • Purification and identification of the bulk compounds are accomplished in a fully automatic manner by high-performance liquid chromatography (HPLC) and mass spectrometry (MS). Specifically, the compounds are purified by LC and simultaneously identified based on detection of (M+1) + by MS.
  • Compound (1a) used in the above scheme may be synthesized by Production Scheme B or Production Scheme C below.
  • M represents a carboxylic acid group, an acid chloride group, a sulfonyl chloride group or an isocyanate group
  • U represents -CO-, -SO 2 - or -NHCO-.
  • compound (1b) and compound (2b) are reacted in a solvent (for example, THF, methylene chloride, acetonitrile or DMF) in the presence of a base to obtain compound (3b).
  • bases there may be mentioned basic salts such as sodium carbonate, potassium carbonate, cesium carbonate and the like, inorganic bases such as sodium hydroxide, potassium hydroxide, potassium fluoride and the like, aromatic amines such as pyridine, lutidine and the like, tertiary amines such as triethylamine, tripropylamine, tributylamine, cyclohexyldimethylamine, diisopropylethylamine, 4-dimethylaminopyridine, N,N-dimethylaniline, N-methylpiperidine, N-methylpyrrolidine, N-methylmorpholine and the like, alkali metal hydrides such as sodium hydride, potassium hydride and the like, metal amides such as sodium amide, lithium di
  • the reaction may be conducted for 0.5 to 24 hours at a temperature from -80 to 150°C and preferably -50 to 120°C.
  • the product may be used either directly as the reaction solution or as a crude substance for the subsequent reaction, or it may be isolated from the reaction mixture according to ordinary methods, or easily purified by separating means such as recrystallization, distillation, chromatography or the like.
  • Compound (3b) and compound (4b) may be coupled according to the procedure described in Tetrahedron Lett., Vol.33, p.7433, 1992 to obtain compound (5b). Specifically, compound (3b) and compound (4b) are reacted in the presence of a palladium catalyst and base.
  • the product may be used either directly as the reaction solution or as a crude substance for the subsequent reaction, or it may be isolated from the reaction mixture according to ordinary methods, or easily purified by separating means such as recrystallization, distillation or chromatography.
  • Compound (3b) and compound (6b) may be used to obtain compound (7b) by the same method as in Step 2B above.
  • Compound (7b) may be oxidized in a solvent (for example, acetonitrile or dichloromethane) in the presence of an oxidizing agent, scavenger and buffer to obtain a carboxylic acid.
  • a solvent for example, acetonitrile or dichloromethane
  • an oxidizing agent for example, acetonitrile or dichloromethane
  • scavenger there may be used 2-methyl-2-butene, sulfamic acid, or the like.
  • the buffer may be sodium dihydrogen phosphate dihydrate.
  • the obtained carboxylic acid may be esterified according to the procedure described in T.W. Greene, Protective Groups in Organic Synthesis, Wiley, New York, 1991, to obtain compound (5b).
  • the compound may be reacted in a solvent (for example, toluene) in the presence of N,N-dimethylformamide and di-t-butyl acetal.
  • Compound (5b) may be reduced according to the same method described in Synlett, Vol.9, p.1028, 1998 to obtain compound (8b). Specifically, compound (5b) may be reacted in a solvent (for example, ethanol) in the presence of iron and ammonium chloride. Alternatively, it may be reacted in a solvent (for example, ethanol) in the presence of zinc and acetic acid to obtain compound (8b).
  • a solvent for example, ethanol
  • zinc and acetic acid to obtain compound (8b).
  • the product may be used either directly as the reaction solution or as a crude substance for the subsequent reaction, or it may be isolated from the reaction mixture according to ordinary methods, or easily purified by separating means such as recrystallization, distillation, chromatography or the like.
  • Compound (8b) and R 1 -M (9b) (where M represents COCl, SO 2 Cl or NCO) or (R 1 -CO) 2 O (10b) may be reacted in a solvent (for example, tetrahydrofuran (hereinafter abbreviated as THF), methylene chloride, acetonitrile, dimethylformamide (hereinafter abbreviated as DMF) or toluene) in the presence of a base to obtain compound (11b).
  • a solvent for example, tetrahydrofuran (hereinafter abbreviated as THF), methylene chloride, acetonitrile, dimethylformamide (hereinafter abbreviated as DMF) or toluene
  • THF tetrahydrofuran
  • DMF dimethylformamide
  • toluene dimethylformamide
  • aromatic amines such as pyridine, lutidine and the like
  • tertiary amines such as triethylamine, tripropylamine, tributylamine, cyclohexyldimethylamine, diisopropylethylamine, 4-dimethylaminopyridine, N,N-dimethylaniline, N-methylpiperidine, N-methylpyrrolidine and N-methylmorpholine.
  • the reaction may be conducted for 0.5 to 24 hours, and preferably 1-12 hours, at a temperature from -40 to 80°C and preferably -10 to 60°C.
  • the product may be used either directly as the reaction solution or as a crude substance for the subsequent reaction, or it may be isolated from the reaction mixture according to ordinary methods, or easily purified by separating means such as recrystallization, distillation, chromatography or the like.
  • compound (8b) and R 1 -M (9b) may be reacted in a solvent (for example, DMF, THF, toluene or methylene chloride) in the presence of a condensing agent and a base to obtain compound (11b).
  • a solvent for example, DMF, THF, toluene or methylene chloride
  • DCC 1,3-dicyclohexyl carbodiimide
  • EDC 1-ethyl-3-[3-(dimethylamino)propyl] carbodiimide
  • THFH tetramethylfluoroformamidium hexafluorophosphate
  • HATU O-(7-azabenzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate
  • aromatic amines such as pyridine, lutidine and the like
  • tertiary amines such as triethylamine, tripropylamine, tributylamine, cyclohexyldimethylamine, diisopropylethylamine, 4-dimethylaminopyridine, N,N-dimethylaniline, N-methylpiperidine, N-methylpyrrolidine, N-methylmorpholine and the like.
  • the reaction may be conducted for 0.5 to 24 hours, and preferably 1-12 hours, at a temperature from -40 to 80°C and preferably -10 to 60°C.
  • the product may be used either directly as the reaction solution or as a crude substance for the subsequent reaction, or it may be isolated from the reaction mixture according to ordinary methods, or easily purified by separating means such as recrystallization, distillation, chromatography or the like.
  • halogen atom (Hal) of compound (5c) may be converted to a cyano group by the same method described in Synthetic Communications, Vol.24, p.889, 1994, to obtain compound (3c). Specifically, compound (5c) may be reacted in a solvent (for example, DMF) in the presence of zinc cyanide and a palladium catalyst.
  • a solvent for example, DMF
  • Compound (3c) may be used to obtain compound (6c) by the same method as Step 5B above.
  • Compound (7c) may be obtained from compound (6c) using the Sandmeyer method described in Synthetic Communications, Vol.22, (21), p.3067, 1992. Specifically, compound (6c) and a nitrous acid ester may be reacted in the presence of a copper halide.
  • the product may be used as a crude substance for the subsequent reaction, or it may be isolated from the reaction mixture according to ordinary methods, or easily purified by separating means such as recrystallization, distillation, chromatography or the like.
  • Compound (7c) and compound (8c) may be used to obtain compound (9c) by the same method as Step 2B above.
  • the reaction product may be purified by any desired ordinary treatment method, for example, by column chromatography using silica gel, an adsorbing resin or the like, or recrystallization from a suitable solvent. Purification and identification of the various compounds in each of the steps may be accomplished in a fully automatic manner by HPLC and MS, where purification by HPLC is conducted simultaneously with identification of the compounds based on detection of (M+1) + by MS.
  • amidino derivative of the invention its pharmacologically acceptable salt or its solvate which is obtained in this manner may be used as a compound library. Further a set of 1 to 1 million amidino derivatives or their pharmacologically acceptable salts or their solvates may be used as a compound library.
  • Blood clotting factor VIIa inhibitors, anticoagulants and thrombosis therapeutic agents will now be explained.
  • the amidino derivatives of the invention and their pharmacologically acceptable salts and solvates inherently have serine protease inhibiting activity, and exhibit excellent inhibiting activity particularly against blood clotting factor VIIa.
  • the amidino derivatives of the invention and their pharmacologically acceptable salts and solvates therefore function as blood clotting factor VIIa inhibitors (blood clotting factor VIIa enzyme activity inhibitors), and are hence useful as prophylactic and/or therapeutic agents for clinical conditions involving thrombogenesis in which extrinsic blood clotting mechanisms are implicated, i.e. as anticoagulants (particularly anticoagulants with inhibiting activity against blood clotting factor VIIa) or as thrombosis therapeutic agents.
  • thrombosis As illnesses that may be prevented and/or treated by formulations according to the invention there may be mentioned thrombosis, deep venous thrombosis, pulmonary embolism, acute myocardial infarction, unstable angina, cerebral thrombosis, restenosis, arteriosclerosis and glomerulosclerosis.
  • the blood clotting factor VIIa inhibitors of the invention comprise the aforementioned amidino derivatives of the invention or their pharmacologically acceptable salts or solvates.
  • the anticoagulants of the invention contain the aforementioned amidino derivatives of the invention or their pharmacologically acceptable salts or solvates as active ingredients.
  • the thrombosis therapeutic agents of the invention also contain the aforementioned amidino derivatives of the invention or their pharmacologically acceptable salts or solvates as active ingredients.
  • the anticoagulants and thrombosis therapeutic agents of the invention may be formulated using ordinary formulation techniques, for use in the form of solid, semi-solid or liquid pharmaceutical formulations.
  • the active ingredient an amidino derivative of the invention or its pharmacologically acceptable salt or solvate
  • ordinary medicinally acceptable atoxic carriers to prepare any desired dosage form such as tablets, pellets, lozenges, capsules, granules, syrup, powder, suppository, cream, ointment, injection or the like.
  • adjuvants, stabilizers, thickeners, preservatives, osmotic regulating salts, buffering agents, coloring agents, aromas or the like may also be added to the formulation.
  • the content of the active ingredient in an anticoagulant or thrombosis therapeutic agent according to the invention is not particularly restricted, and may be appropriately selected depending on the dosage form. Also, two or more different active ingredients may be used in combination in an anticoagulant or thrombosis therapeutic agent of the invention.
  • a blood clotting factor VIIa inhibitor, anticoagulant or thrombosis therapeutic agent of the invention may be orally, enterally or intravenously administered, depending on the dosage form.
  • the administered dose will differ considerably depending on the type of illness, severity of symptoms, patient age and gender, sensitivity to the drug agent and the like, but the daily dose for adults will generally be between about 0.03 mg and about 1000 mg, preferably between about 0.1 mg and about 500 mg and even more preferably between about 0.1 mg and about 100 mg.
  • the dose may be administered at once or in divided doses per day, or at once or in divided doses every several days. In the case of injection, the dose will usually be from about 1 ⁇ g to about 3000 ⁇ g and preferably from about 3 ⁇ g to about 1000 ⁇ g per kg of body weight.
  • the title compound was obtained by the same process as in Production Example 3-1, except that pinacol 4-[(2-methylpropyl)carbamoyl-3-methoxycarbonylphenylboronate was used instead of 2-formylphenylboronic acid.
  • Methanesulfonyl chloride (8 mg) was added to a pyridine solution containing the compound (t-butyl 5-amino-2-(6-cyano-2-naphthyloxy)benzoate) (20 mg) obtained in a previous production example, and reaction was conducted at 50°C for 1 hour. The reaction solution was concentrated, a saturated sodium bicarbonate solution was added to the residue, and extraction was performed with ethyl acetate. After freezing the aqueous layer with ethanol-dry ice, the ethyl acetate layer was decanted and the solvent was distilled off.
  • Example Compound name 1-2 2-(6-amidino-2-naphthyloxy)-5-(4-methoxybenzenesulfonyl) aminobenzoic acid trifluoroacetate 1-3 2-(6-amidino-2-naphthyloxy)-5-(4-fluorobenzenesulfonyl) aminobenzoic acid trifluoroacetate 1-4 2-(6-amidino-2-naphthyloxy)-5-(2-naphthalenesulfonyl) aminobenzoic acid trifluoroacetate 1-5 2-(6-amidino-2-naphthyloxy)-5-(2,3-dichlorobenzene sulfonyl)aminobenzoic acid trifluoroacetate 1-6 2-(6-amidino-2-naphthyloxy)-5-[4-(phenylsulfonyl) thiophene-2-sulfonyl]a
  • Example 1-34 2'-(6-amidino-2-naphthyloxy)-5'-(3,4-dimethoxybenzenesulfonyl)amino-1,1'-biphenyl-2 -carboxylic acid trifluoroacetate
  • Example Compound name 2-1 2-(6-amidino-2-naphthyloxy)-4-(4-methoxybenzenesulfonyl) aminobenzoic acid trifluoroacetate 2-2 2-(6-amidino-2-naphthyloxy)-4-(4-fluorobenzenesulfonyl) aminobenzoic acid trifluoroacetate 2-3 2-(6-amidino-2-naphthyloxy)-4-(2-naphthalenesulfonyl) aminobenzoic acid trifluoroacetate 2-4 2-(6-amidino-2-naphthyloxy)-4-(3,4-dichlorobenzene sulfonyl)aminobenzoic acid trifluoroacetate
  • Example Compound name 3-1 2-(6-amidino-2-naphthyloxy)-5-(2-thienylcarbonyl) aminobenzoic acid trifluoroacetate 3-2 2'-(4-amidinophenoxy)-5'-benzoylamino-1,1'-biphenyl-2-carboxylic acid trifluoroacetate 3-3 2'-(4-amidinophenoxy)-5'-benzoylamino-1,1'-biphenyl-3-carboxylic acid trifluoroacetate
  • Example Compound name 4-1 2'-(4-amidinophenoxy)-5'-(3-methylbutanoyl)amino-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
  • 4-2 2'-(4-amidinophenoxy)-5'-(3-methylbutanoyl)amino-1,1'-biphenyl-3-carboxylic acid trifluoroacetate
  • the obtained residue was dissolved in acetic acid (1 ml), acetic anhydride (8 mg) was added, and reaction was conducted at room temperature for 15 minutes. Next, 10% palladium-carbon (1 mg) was added thereto and catalytic reduction was carried out for 6 hours under ordinary pressure. The catalyst was then filtered off using celite, and the filtrate was concentrated. The residue was dissolved in trifluoroacetic acid:dichloromethane (1:1, 1 ml) and reaction was conducted at room temperature for 90 minutes.
  • Example Compound name 6-1 2'-(6-amidino-2-naphthyloxy)-5'-(3-chlorophenylureido)-1,1'-biphenyl-2-carboxylic acid trifluoroacetate 6-2 2'-(6-amidino-2-naphthyloxy)-5'-(3-methoxyphenylureido)-1,1'-biphenyl-2-carboxylic acid trifluoroacetate 6-3 2'-(6-amidino-2-naphthyloxy)-5'-(cyclohexylureido)-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
  • Example 8-1 2'-(6-amidino-2-naphthyloxy)-4-(2-methylpropyl carbamoyl)-5'-methanesulfonylamino-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
  • Methanesulfonyl chloride (10 ⁇ L) was added to a pyridine solution (1 mL) containing the compound (methyl 5'-amino-2'-(6-cyano-2-naphthyloxy)-4-[(2-methylpropyl)carbamoyl]-1,1'-biphenyl-2-carboxylic acid) (20 mg) obtained in a previous production example, and the mixture was stirred at room temperature overnight. Nitrogen gas was sprayed into the reaction solution which was then concentrated. Ethyl acetate and saturated sodium bicarbonate water were added to the residue, and the organic layer was separated off. Next, nitrogen gas was sprayed into the organic layer which was then concentrated.
  • Example Compound name 8-2 2'-(6-amidino-2-naphthyloxy)-4-(2-methylpropylcarbamoyl)-5'-benzenesulfonylamino-1,1'-biphenyl-2-carboxylic acid trifluoroacetate 8-3 2'-(6-amidino-2-naphthyloxy)-4-(2-methylpropylcarbamoyl)-5'-(3-chloro-4-methylbenzenesulfonyl)amino-1,1'-biphenyl-2-carboxylic acid trifluoroacetate 8-4 2'-(6-amidino-2-naphthyloxy)-4-(2-methylpropylcarbamoyl)-5'-(3,4-dichlorobenzenesulfonyl)amino-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
  • Example Compound name 9-1 2'-(6-amidino-2-naphthyloxy)-4-(2-methylpropylcarbamoyl)-4'-methyl-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
  • 9-2 2'-(4-amidino-2-phenoxy)-4-(2-methylpropylcarbamoyl)-4'-methyl-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
  • the composition of the reaction buffer was 50 mM Tris-acetate (pH 7.5), 15 mM calcium chloride (CaCl 2 ), 0.15 M sodium chloride (NaCl) and 6 mg/ml Cephalin. There were also added to the buffer 10 nM human tissue factor (TF) and 5 nM human blood clotting factor VIIa, to prepare enzyme solutions. After separating off 65 ⁇ l of each solution, a 10 ⁇ l portion of the 2% dimethyl sulfoxide solution of each compound of the invention was added, and preincubation was performed at room temperature. As a substrate there was added 25 ⁇ l of 1.0 mM Spectrozyme VII, and reaction was conducted for 40 minutes at room temperature. The enzyme reaction rate was quantified by the change in absorbance of released 4-nitroanilide at 405 nm.
  • the enzyme reaction rate was determined in the presence of the above-mentioned compounds of the present invention from 10 ⁇ g/ml to 0.1 ng/ml.
  • the IC 50 values were calculated based on nonlinear regression analysis, as an index of the inhibiting activity against human blood clotting factor VIIa. The results are shown in Table 32.
  • amidino derivatives of the present invention and their pharmacologically acceptable salts or solvates are useful as prophylactic and/or therapeutic agents for clinical conditions involving thrombogenesis in which extrinsic clotting mechanisms are implicated, and therefore the present invention provides blood clotting factor VIIa inhibitors, anticoagulants (particularly anticoagulants with inhibiting activity against blood clotting factor VIIa) and thrombosis therapeutic agents which are effective for prevention and/or treatment of clinical conditions involving thrombogenesis.

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Abstract

Amidino derivatives represented by the following general formula (I):
Figure 80000001
{where X is a group represented by R1SO2NR2- (wherein R1 represents optionally substituted C6-14 aryl, etc. and R2 represents hydrogen atom, etc.), etc., Ar1 represents 2,6-naphthylene, etc., R3 represents hydrogen atom, etc. and Y represents carboxyphenyl, etc.}
and their pharmacologically acceptable salts or solvates.

Description

    BACKGROUND OF THE INVENTION Field of the Invention
  • The present invention relates to medicinally useful novel amidino derivatives, to their pharmacologically acceptable salts or solvates, and to blood clotting factor VIIa inhibitors, anticoagulants and thrombosis therapeutic agents containing them as active ingredients.
    • Related Background Art
  • Destruction of blood vessels in the body triggers rapid production of thrombin to prevent death by bleeding. On the other hand, overproduction of thrombin accompanying inflammation reaction at damaged blood vessels leads to thrombosis, which impairs the functioning of important organs. Thrombin inhibitors such as heparin and warfarin, which inhibit thrombin production or directly inhibit thrombin activity, have long been used as anticoagulants for treatment or prevention of thrombosis. However, because such drugs have not been very satisfying from a therapeutic standpoint, research and development have been pursued on a worldwide scale for new anticoagulants with excellent dose response, low risk of hemorrhage and suitability for oral administration.
  • Incidentally, mechanisms of blood clotting had been divided into two types, of the "intrinsic coagulation pathway" which is initiated by activation of Factor XII (FXII) by contact with negatively charged substances, or of the "extrinsic coagulation pathway" which is activated by Tissue Factor (TF) and Factor VII (FVII). It is extrinsic coagulation that is implicated in thrombosis because of specific expression of TF observed in the clinical condition. In the blood clotting cascade, compounds that inhibit blood clotting factor VIIa, which is furthest upstream in the extrinsic coagulation pathway, are believed to be useful as prophylactic and/or therapeutic agents for clinical conditions involving thrombogenesis in which extrinsic clotting mechanisms are implicated. In addition, blood clotting factor VIIa inhibitors, unlike thrombin inhibitors, are expected to produce milder hemorrhage since the intrinsic coagulation pathway still remains, and are thus believed to be useful from this viewpoint as well.
  • Such compounds that have been conventionally known to inhibit blood clotting factor VIIa include amidinonaphthol derivatives (Tetrahedron, Vol.55, p.6219, 1999), amidino derivatives (International Publication No. WO99/41231) N-sulfonyl dipeptide derivatives (International Publication No. WO00/58346), 6-[[(allyl)oxy]methyl]naphthalene-2-carboximidamide derivatives (International Publication No. WO00/66545), and the like.
  • However, none of the publicly known compounds of the prior art have given satisfactory results in terms of inhibiting activity against blood clotting factor VIIa.
    • SUMMARY OF THE INVENTION
  • It is an object of the present invention, which has been accomplished in light of the aforementioned problems of the prior art, to provide novel amidino derivatives having serine protease inhibiting activity and especially excellent inhibiting activity against blood clotting factor VIIa, as well as their pharmacologically acceptable salts or solvates, and blood clotting factor VIIa inhibitors, anticoagulants and thrombosis therapeutic agents containing them.
  • As a result of avid research directed toward achieving the object stated above, the present inventors have completed the invention upon discovering that novel amidino derivatives having a specific chemical structure exhibit excellent inhibiting activity against blood clotting factor VIIa.
  • Specifically, the present invention provides:
  • <1> An amidino derivative represented by the following general formula (I):
    Figure 00040001
    {where X represents C1-6 alkyl, a halogen atom, -NH2 or a group represented by the following formula:
    Figure 00040002
    (where R1 represents optionally substituted C1-6 alkyl, optionally substituted C1-6 alkoxy, optionally substituted C3-8 cycloalkyl, optionally substituted C6-14 aryl, optionally substituted C7-20 aralkyl, an optionally substituted 5- to 14-membered aromatic heterocyclic group or a group represented by the following formula:
    Figure 00040003
    (where R4 represents hydrogen atom, C1-6 alkyl or optionally substituted C2-7 acyl),
    R2 represents hydrogen atom or C1-6 alkyl, and
    X2 represents -CO-, -SO2-, -NH-CO- or a single bond),
    R3 represents hydrogen atom, hydroxyl, C2-7 acyl or C2-7 alkoxycarbonyl,
    Y represents a group of the formula -Ar2-CO2R5 (where Ar2 represents optionally substituted C6-14 aryl, an optionally substituted 5- to 14-membered aromatic heterocyclic group or a single bond and R5 represents hydrogen atom or C1-6 alkyl), and
    Ar1 represents 2,6-naphthylene, 1,4-phenylene, 1,3-phenylene, a group represented by the following formula:
    Figure 00050001
    or a group represented by the following formula:
    Figure 00050002
    or a pharmacologically acceptable salt or solvate thereof.
  • <2> An amidino derivative represented by the following general formula (II):
    Figure 00060001
    (where X, Y, Ar1 and R3 have the same definitions as X, Y, Ar1 and R3 in <1>) , or a pharmacologically acceptable salt or solvate thereof.
  • <3> An amidino derivative represented by the following general formula (III):
    Figure 00060002
    (where X, Y and R3 have the same definitions as X, Y and R3 in <1>), or a pharmacologically acceptable salt or solvate thereof.
  • <4> An amidino derivative or a pharmacologically acceptable salt or solvate thereof according to any one of <1> to <3> above wherein R3 is hydrogen atom.
  • <5> An amidino derivative or a pharmacologically acceptable salt or solvate thereof according to any one of <1> to <4> above wherein X is C1-6 alkyl or a group represented by the formula R1-SO2NH- (where R1 has the same definition as R1 in <1>).
  • <6> An amidino derivative or a pharmacologically acceptable salt or solvate thereof according to any one of <1> to <5> above wherein X is a group represented by the formula R1-SO2NH- {where R1 represents optionally substituted C6-14 aryl or a group represented by the following formula:
    Figure 00070001
    (where R4 represents optionally substituted C2-7 acyl)}.
  • <7> An amidino derivative or a pharmacologically acceptable salt or solvate thereof according to any one of <1> to <6> above wherein Y is carboxyl or optionally substituted carboxyphenyl.
  • <8> An amidino derivative or a pharmacologically acceptable salt or solvate thereof according to any one of <1> to <7> above wherein Y is a group represented by the following formula:
    Figure 00080001
  • <9> An amidino derivative or a pharmacologically acceptable salt or solvate thereof according to <1>, wherein X represents C1-6 alkyl, a halogen atom, -NH2 or a group represented by the following formula:
    Figure 00080002
    {where R1 represents C1-6 alkyl, C1-6 alkoxy, C3-8 cycloalkyl, C6-14 aryl, C7-20 aralkyl, a 5- to 14-membered aromatic heterocyclic group or a group represented by the following formula:
    Figure 00080003
    (where R4 represents hydrogen atom, C1-6 alkyl or C2-7 acyl) and R1 optionally has 1-3 substituents selected from the following substituent group A,
    R2 represents hydrogen atom or C1-6 alkyl, and X2 represents -CO-, -SO2-, -NH-CO- or a single bond}.
    • (Substituent Group A)
  • The group consisting of halogen atoms, C1-6 alkyl, C1-6 alkoxy, C1-6 alkylsulfonyl, C3-8 cycloalkyl, C3-8 cycloalkoxy, halo C1-6 alkyl, halo C1-6 alkoxy, C6-14 aryl, C1-6 alkoxy-C6-14 aryl, C6-14 aryloxy, C6-14 arylsulfonyl, nitro, C1-6 alkoxycarbonyl, carboxyl, acetamide, hydroxyl, 5- to 14-membered aromatic heterocyclic groups, CF3-, CF3O-, Z-CO- {where Z represents -NR7R8 (wherein R7 and R8 may be the same or different and each represents hydrogen atom, C1-6 alkyl, C3-8 cycloalkyl, C6-14 aryl, benzyl or C3-8 cycloalkylmethyl), 1-pyrrolidinyl or 1-piperidyl}, amino C1-6 alkyl and C1-6 alkylenedioxy.
  • <10> An amidino derivative or a pharmacologically acceptable salt or solvate thereof according to <9>, wherein substituent group A is the group consisting of halogen atoms, C1-6 alkyl, C1-6 alkoxy, C1-6 alkylsulfonyl, halo C1-6 alkyl, halo C1-6 alkoxy, C6-14 arylsulfonyl, nitro, carboxyl, acetamide, Z-CO- {where Z represents -NR7R8 (wherein R7 and R8 may be the same or different and each represents hydrogen atom or C1-6 alkyl)} and C1-6 alkylenedioxy.
  • <11> An amidino derivative or a pharmacologically acceptable salt or solvate thereof according to <1>, wherein Y represents a group represented by the formula -Ar3-CO2R5 (where Ar3 represents C6-14 aryl or a 5- to 14-membered aromatic heterocyclic group, and R5 represents hydrogen atom or C1-6 alkyl) or a group represented by the formula -CO2R5 (wherein R5 has the same definition as above), and Ar3 optionally has 1-3 substituents selected from the following substituent group B.
    • (Substituent Group B)
  • The group consisting of halogen atoms, C1-6 alkyl, C1-6 alkoxy, C1-6 alkylsulfonyl, C3-8 cycloalkyl, C3-8 cycloalkoxy, halo C1-6 alkyl, halo C1-6 alkoxy, C6-14 aryl, C1-6 alkoxy-C6-14 aryl, C6-14 aryloxy, C6-14 arylsulfonyl, nitro, C1-6 alkoxycarbonyl, carboxyl, acetamide, hydroxyl, 5- to 14-membered aromatic heterocyclic groups, CF3-, CF3O-, Z-CO- {where Z represents -NR7R8 (wherein R7 and R8 may be the same or different and each represents hydrogen atom, C1-6 alkyl, C3-8 cycloalkyl, C6-14 aryl, benzyl or C3-8 cycloalkylmethyl), 1-pyrrolidinyl or 1-piperidyl}, amino C1-6 alkyl and C1-6 alkylenedioxy.
  • <12> An amidino derivative or a pharmacologically acceptable salt or solvate thereof according to <11>, wherein substituent group B is the group consisting of halogen atoms, C1-6 alkyl, C1-6 alkoxy, C1-6 alkylsulfonyl, halo C1-6 alkyl, halo C1-6 alkoxy, C6-14 arylsulfonyl, nitro, carboxyl, acetamide, Z-CO- {where Z represents -NR7R8 (wherein R7 and R8 may be the same or different and each represents hydrogen atom or C1-6 alkyl) and C1-6 alkylenedioxy.
  • <13> A blood clotting factor VIIa inhibitor comprising an amidino derivative or a pharmacologically acceptable salt or solvate thereof according to any one of <1> to <8>.
  • <14> An anticoagulant containing, as an active ingredient, an amidino derivative or a pharmacologically acceptable salt or solvate thereof according to any one of <1> to <8>.
  • <15> A thrombosis therapeutic agent containing, as an active ingredient, an amidino derivative or a pharmacologically acceptable salt or solvate thereof according to any one of <1> to <8>.
  • <16> A compound library comprising an amidino derivative or a pharmacologically acceptable salt or solvate thereof according to any one of <1> to <8>.
  • <17> A compound library comprising 1 to 1 million (preferably 8 to 1 million, more preferably 16 to 1 million and even more preferably 96 to 1 million) amidino derivatives or their pharmacologically acceptable salts or solvates thereof according to any of <1> to <8>.
  • <18> The use of an amidino derivative or a pharmacologically acceptable salt or solvate thereof according to any one of <1> to <8> as a blood clotting factor VIIa inhibitor.
  • <19> The use of an amidino derivative or a pharmacologically acceptable salt or solvate thereof according to any one of <1> to <8> as an active ingredient of an anticoagulant.
  • <20> The use of an amidino derivative or a pharmacologically acceptable salt or solvate thereof according to any one of <1> to <8> as an active ingredient of a thrombosis therapeutic agent.
  • <21> A treatment method for clinical conditions involving thrombogenesis in which extrinsic blood clotting mechanisms are implicated, which involves administering an amidino derivative or a pharmacologically acceptable salt or solvate thereof according to any one of <1> to <8>.
  • <22> A prophylactic method for clinical conditions involving thrombogenesis in which extrinsic blood clotting mechanisms are implicated, which involves administering an amidino derivative or a pharmacologically acceptable salt or solvate thereof according to any one of <1> to <8>.
    • DESCRIPTION OF THE PREFERRED EMBODIMENTS
  • The present invention will now be explained in further detail. First, the amidino derivatives of the invention and their pharmacologically acceptable salts or solvates will be explained.
  • The amidino derivatives of the invention are represented by general formula (I) given above. There are no particular restrictions on the positions of the group represented by X and the group represented by Y bonded to the benzene ring in general formula (I), but for more enhanced pharmacological activity, preferably an ether bond is formed at the carbon atom at position 2 of the Y-substituted benzene (where the Y-bonded benzene carbon atom is position 1) and a group represented by X is bonded at the carbon atom at position 4 or 5 (most preferably position 5). Preferred, therefore, are amidino derivatives represented by the following general formula (II):
    Figure 00130001
    (where X, Y, Ar1 and R3 have the same definitions as X, Y, Ar1 and R3 for general formula (I)), or pharmacologically acceptable salts or solvates thereof.
  • The group represented by Ar1 in general formula (I) is 2,6-naphthylene, 1,4-phenylene, 1,3-phenylene, a group represented by the following formula:
    Figure 00140001
    or a group represented by the following formula:
    Figure 00140002
    and for more enhanced pharmacological activity it is preferably 2,6-naphthylene. Preferred, therefore, are amidino derivatives represented by the following general formula (III):
    Figure 00140003
    (where X, Y and R3 have the same definitions as X, Y, and R3 for general formula (I)), or pharmacologically acceptable salts or solvates thereof.
  • Also, X in general formula (I) is C1-6 alkyl, a halogen atom, -NH2 or a group represented by the following formula (IV):
    Figure 00150001
    {where R1 represents optionally substituted C1-6 alkyl, optionally substituted C1-6 alkoxy, optionally substituted C3-8 cycloalkyl, optionally substituted C6-14 aryl, optionally substituted C7-20 aralkyl, an optionally substituted 5- to 14-membered aromatic heterocyclic group or a group represented by the following formula (V):
    Figure 00150002
    (where R4 represents hydrogen atom, C1-6 alkyl or optionally substituted C2-7 acyl),
    R2 represents hydrogen atom or C1-6 alkyl, and
    X2 represents -CO-, -SO2-, -NH-CO- or a single bond}, but for more enhanced pharmacological activity it is preferably C1-6 alkyl or a group represented by the aforementioned formula (IV) (for example, R1-NHCONR2-, R1-CONR2-, R1-SO2NR2-), more preferably C1-6 alkyl (especially methyl) or R1-SO2NR2-, and most preferably R1-SO2NR2-.
  • The group represented by X2 in general formula (IV) is -CO-, -SO2-, -NH-CO- or a single bond, but for more enhanced pharmacological activity it is preferably -CO-, -SO2- or -NH-CO-, and most preferably -SO2-.
  • The group represented by R1 in general formula (IV) is (i) optionally substituted C1-6 alkyl, (ii) optionally substituted C1-6 alkoxy, (iii) optionally substituted C3-8 cycloalkyl, (iv) optionally substituted C6-14 aryl, (v) optionally substituted C7-20 aralkyl, (vi) an optionally substituted 5- to 14-membered aromatic heterocyclic group or (vii) a group represented by formula (V) above, and it is preferably (i), (iii), (iv), (v), (vi) or (vii); for more enhanced pharmacological activity it is most preferably (iv) or (vii).
  • The group represented by R4 in formula (V) above is hydrogen atom, C1-6 alkyl or optionally substituted C2-7 acyl, but for more enhanced pharmacological activity it is preferably optionally substituted C2-7 acyl.
  • The group represented by R2 in formula (IV) above is hydrogen atom or C1-6 alkyl, but for more enhanced pharmacological activity it is preferably hydrogen atom.
  • The group represented by R3 in general formula (I) is hydrogen atom, hydroxyl, C2-7 acyl or C2-7 alkoxycarbonyl, but for more enhanced pharmacological activity it is preferably hydrogen atom.
  • The group represented by Y in general formula (I) is a group represented by the formula -Ar2-COOR5 (where Ar2 represents optionally substituted C6-14 aryl, an optionally substituted 5- to 14-membered aromatic heterocyclic group or a single bond, and R5 represents hydrogen atom or C1-6 alkyl). For more enhanced pharmacological activity, the group represented by Y is preferably a carboxyl group (-COOH) or a group represented by the formula -Ar2-COOH (where Ar2 represents optionally substituted C6-14 aryl), more preferably carboxyl or optionally substituted carboxyphenyl, and most preferably a group represented by the following formula:
    Figure 00170001
  • The term "C1-6 alkyl" used throughout the present specification refers to a linear or branched alkyl group of 1-6 carbons (preferably 1-4 carbons), such as methyl, ethyl, n-propyl, i-propyl, n-butyl, s-butyl, t-butyl, pentyl, isopentyl, hexyl or the like.
  • The term "C1-6 alkoxy" refers to a linear or branched alkoxy group of 1-6 carbons (preferably 1-4 carbons), such as methoxy, ethoxy, n-propoxy, i-propoxy, n-butoxy, s-butoxy, t-butoxy, pentoxy, isopentoxy, hexoxy or the like.
  • The term "C3-8 cycloalkyl" refers to a cyclic saturated hydrocarbon group of 3-8 carbons (preferably 3-6 carbons) such as cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl or the like.
  • The term "C6-14 aryl" refers to a monovalent aromatic hydrocarbon group of 6-14 carbons (preferably 6-10 carbons) lacking one of its hydrogen atoms, such as phenyl, biphenyl, naphthyl, anthranyl, phenanthryl, tolyl, xylyl or the like, with phenyl and naphthyl being preferred for more enhanced pharmacological activity. The C6-14 aryl group referred to here need not necessarily be monovalent, and it may be a divalent group lacking yet another hydrogen atom.
  • The term "C7-20 aralkyl"refers to a group which is an alkyl group substituted with an aryl group at one of its hydrogen atoms, and has 7-20 (preferably 7-14) carbons, such as benzyl, phenethyl or the like.
  • The term "5- to 14-membered aromatic heterocyclic group" refers to a 5- to 14-membered aromatic heterocyclic group having at least one hetero atom (nitrogen atom, oxygen atom, sulfur atom, etc.), such as thienyl, pyridyl, imidazolyl, pyrazinyl, pyrimidyl or the like. The 5- to 14-membered aromatic heterocyclic group referred to here need necessarily not be monovalent, and it may be a divalent group lacking yet another hydrogen atom.
  • The term "C2-7 acyl" refers to a carbonyl group substituted with an alkyl group, aryl group, alkoxy group, etc. and having 2-7 carbons, and is preferably a carbonyl group bonded to a C1-6 alkyl group. Examples of such C2-7 acyl groups include alkylcarbonyl groups such as acetyl, propionyl, butanoyl, pivaloyl and cyclohexanecarbonyl and arylcarbonyl groups such as benzoyl, among which acetyl, propionyl and butanoyl are preferred.
  • The term "C2-7 alkoxycarbonyl" refers to a carbonyl group bonded to a C1-6 alkoxy group as defined above and having 2-7 carbons, such as methoxycarbonyl, ethoxycarbonyl or the like.
  • The term "halogen atom" refers to a fluorine atom, chlorine atom, bromine atom or iodine atom.
  • The term "optionally substituted" as used throughout the present specification means "optionally having one or more substituents in any desired combination at substitutable positions". As specific such substituents there may be mentioned hydrogen atom, halogen atoms (fluorine, chlorine, bromine and iodine), C1-6 alkyl, C1-6 alkoxy, C1-6 alkylsulfonyl, C3-8 cycloalkyl, C3-8 cycloalkoxy, halo C1-6 alkyl, halo C1-6 alkoxy, C6-14 aryl, C1-6 alkoxy-C6-14 aryl, C6-14 aryloxy, C6-14 arylsulfonyl, nitro, C1-6 alkoxycarbonyl, carboxyl, acetamide, hydroxyl, 5- to 14-membered aromatic heterocyclic groups, CF3-, CF3O-, Z-CO- {where Z represents -NR7R8 (wherein R7 and R8 may be the same or different and each represents hydrogen atom, C1-6 alkyl, C3-8 cycloalkyl, C6-14 aryl, benzyl or C3-8 cycloalkylmethyl), 1-pyrrolidinyl or 1-piperidyl}, amino C1-6 alkyl, C1-6 alkylenedioxy and the like, among which there are preferred halogen atoms, C1-6 alkyl, C1-6 alkoxy, C1-6 alkylsulfonyl, halo C1-6 alkyl, halo C1-6 alkoxy, C6-14 arylsulfonyl, nitro, carboxyl, acetamide, Z-CO- {where Z represents -NR7R8 (wherein R7 and R8 may be the same or different and each represents hydrogen atom or C1-6 alkyl)} and C1-6 alkylenedioxy. These substituents may also be bonded together to form rings. The number of substituents need only be within the range of the number of substituent-bindable positions, with 1-3 being preferred.
  • The scope of "pharmacologically acceptable salt" according to the present specification is not particularly restricted, and for example, there may be mentioned salts of inorganic acids, salts of organic acids, salts of inorganic bases, salts of organic bases, salts of acidic or basic amino acids and the like. As preferred examples of salts of inorganic acids there may be mentioned salts of hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid and the like, and as preferred examples of salts of organic acids there may be mentioned salts of acetic acid, succinic acid, fumaric acid, maleic acid, tartaric acid, citric acid, lactic acid, stearic acid, benzoic acid, methanesulfonic acid, p-toluenesulfonic acid and the like. As preferred examples of salts of inorganic bases there may be mentioned alkali metal salts such as sodium salts, potassium salts and the like, alkaline earth metal salts such as calcium salts, magnesium salts and the like, and aluminum salts, ammonium salts, or the like. As preferred examples of salts of organic bases there may be mentioned salts of diethylamine, diethanolamine, meglumine, N,N'-dibenzylethylenediamine and the like. As preferred examples of salts of acidic amino acids there may be mentioned salts of aspartic acid, glutamic acid and the like, and as preferred examples of salts of basic amino acids there may be mentioned salts of arginine, lysine, ornithine and the like.
  • The aforementioned acids or bases are those that form salts at an appropriate ratio in a range of 0.1-5 molecules per molecule of the compound. The pharmacologically acceptable salts of the amidino derivatives of the invention also include prodrugs, and for example, the aforementioned amidino derivative esters may be mentioned, with t-butyl esters being preferred.
  • The amidino derivatives of the invention or their pharmacologically acceptable salts may be in the form of solvates, where such solvates are preferably hydrates.
  • A process for production of the amidino derivatives and their pharmacologically acceptable salts or solvates according to the invention will now be explained. The amidino derivatives of the invention are novel compounds which may be synthesized in the following manner, as an example.
  • Specifically, the amidino derivatives of the invention represented by general formula (I) above may be synthesized utilizing ordinary organic chemical reactions which are already known, and for example, they may be synthesized by the process represented by the reaction scheme (A) shown below, or by a similar process. The symbols of the compounds in the following schemes are the same as defined above.
    Figure 00230001
    • [Step 1A]
  • Compound (1a) may be reacted by the same procedure described in Synthetic Communications, Vol.26 (23), p.4351, 1996 to obtain compound (3a). Specifically, compound (1a) and a hydroxyamine (2a) are reacted in a solvent (for example, methanol or ethanol) in the presence of a base (for example, potassium carbonate).
    • [Step 2A]
  • Compound (3a) may be reacted by the same procedure described in Synthetic Communications, Vol.26 (23), p.4351, 1996 to obtain compound (4a). Specifically, compound (3a) and acetic anhydride are reacted in a solvent (for example, acetic acid), and then a palladium catalyst is added for reaction in hydrogen gas.
    • [Step 3A]
  • Compound (4a) can also be produced by employing the Pinner method. In the publicly known Pinner method, reaction is first conducted from -10°C to 50°C using hydrochloric acid in a solvent (for example, methanol, ethanol or methylene chloride), and then reaction is conducted from -10°C to 50°C using ammonia gas in a solvent (for example, methanol or ethanol).
    • [Step 4A]
  • Compound (4a) may be hydrolyzed in an acid (for example, hydrochloric acid, trifluoroacetic acid or sulfuric acid) to obtain compound (5a). Specifically, compound (4a) is reacted in a solvent (for example, tetrahydrofuran) in the presence of the aforementioned acid.
    • [Step 5A]
  • Compound (1a) may be hydrolyzed in an aqueous solution of a base (for example, sodium hydroxide or lithium hydroxide) to obtain compound (6a). Specifically, compound (1a) is reacted in a solvent (for example, a mixture of tetrahydrofuran and methanol) in the presence of the aforementioned base aqueous solution.
    • [Step 6A]
  • Compound (6a) and compound (2a) may be used to obtain compound (7a) by the same method as Step 1A.
    • [Step 7A]
  • Compound (7a) may be used to obtain compound (5a) by the same method as Step 2A.
  • In order to efficiently obtain each compound, the synthesis may be carried out using combinatorial chemistry. That is, a library for compound (5a) may be constructed by combination of substituent types and positions in compound (5a). Purification and identification of the bulk compounds are accomplished in a fully automatic manner by high-performance liquid chromatography (HPLC) and mass spectrometry (MS). Specifically, the compounds are purified by LC and simultaneously identified based on detection of (M+1)+ by MS.
  • Compound (1a) used in the above scheme may be synthesized by Production Scheme B or Production Scheme C below.
    Figure 00260001
    (In the above scheme, M represents a carboxylic acid group, an acid chloride group, a sulfonyl chloride group or an isocyanate group, and U represents -CO-, -SO2- or -NHCO-.)
    • [Step 1B]
  • Compound (1b) and compound (2b) are reacted in a solvent (for example, THF, methylene chloride, acetonitrile or DMF) in the presence of a base to obtain compound (3b). As bases there may be mentioned basic salts such as sodium carbonate, potassium carbonate, cesium carbonate and the like, inorganic bases such as sodium hydroxide, potassium hydroxide, potassium fluoride and the like, aromatic amines such as pyridine, lutidine and the like, tertiary amines such as triethylamine, tripropylamine, tributylamine, cyclohexyldimethylamine, diisopropylethylamine, 4-dimethylaminopyridine, N,N-dimethylaniline, N-methylpiperidine, N-methylpyrrolidine, N-methylmorpholine and the like, alkali metal hydrides such as sodium hydride, potassium hydride and the like, metal amides such as sodium amide, lithium diisopropylamide, lithium hexamethyldisilazide and the like, and metal alkoxides such as sodium methoxide, sodium ethoxide, potassium tert-butoxide and the like. The reaction may be conducted for 0.5 to 24 hours at a temperature from -80 to 150°C and preferably -50 to 120°C. The product may be used either directly as the reaction solution or as a crude substance for the subsequent reaction, or it may be isolated from the reaction mixture according to ordinary methods, or easily purified by separating means such as recrystallization, distillation, chromatography or the like.
    • [Step 2B]
  • Compound (3b) and compound (4b) may be coupled according to the procedure described in Tetrahedron Lett., Vol.33, p.7433, 1992 to obtain compound (5b). Specifically, compound (3b) and compound (4b) are reacted in the presence of a palladium catalyst and base. The product may be used either directly as the reaction solution or as a crude substance for the subsequent reaction, or it may be isolated from the reaction mixture according to ordinary methods, or easily purified by separating means such as recrystallization, distillation or chromatography.
    • [Step 3B]
  • Compound (3b) and compound (6b) may be used to obtain compound (7b) by the same method as in Step 2B above.
    • [Step 4B]
  • Compound (7b) may be oxidized in a solvent (for example, acetonitrile or dichloromethane) in the presence of an oxidizing agent, scavenger and buffer to obtain a carboxylic acid. Sodium chlorite may be used as the oxidizing agent. As the scavenger there may be used 2-methyl-2-butene, sulfamic acid, or the like. The buffer may be sodium dihydrogen phosphate dihydrate. The obtained carboxylic acid may be esterified according to the procedure described in T.W. Greene, Protective Groups in Organic Synthesis, Wiley, New York, 1991, to obtain compound (5b). For example, the compound may be reacted in a solvent (for example, toluene) in the presence of N,N-dimethylformamide and di-t-butyl acetal.
    • [Step 5B]
  • Compound (5b) may be reduced according to the same method described in Synlett, Vol.9, p.1028, 1998 to obtain compound (8b). Specifically, compound (5b) may be reacted in a solvent (for example, ethanol) in the presence of iron and ammonium chloride. Alternatively, it may be reacted in a solvent (for example, ethanol) in the presence of zinc and acetic acid to obtain compound (8b). The product may be used either directly as the reaction solution or as a crude substance for the subsequent reaction, or it may be isolated from the reaction mixture according to ordinary methods, or easily purified by separating means such as recrystallization, distillation, chromatography or the like.
    • [Step 6B]
  • Compound (8b) and R1-M (9b) (where M represents COCl, SO2Cl or NCO) or (R1-CO)2O (10b) may be reacted in a solvent (for example, tetrahydrofuran (hereinafter abbreviated as THF), methylene chloride, acetonitrile, dimethylformamide (hereinafter abbreviated as DMF) or toluene) in the presence of a base to obtain compound (11b). The use of a solvent in this case is optional. As bases there may be mentioned aromatic amines such as pyridine, lutidine and the like, and tertiary amines such as triethylamine, tripropylamine, tributylamine, cyclohexyldimethylamine, diisopropylethylamine, 4-dimethylaminopyridine, N,N-dimethylaniline, N-methylpiperidine, N-methylpyrrolidine and N-methylmorpholine. The reaction may be conducted for 0.5 to 24 hours, and preferably 1-12 hours, at a temperature from -40 to 80°C and preferably -10 to 60°C. The product may be used either directly as the reaction solution or as a crude substance for the subsequent reaction, or it may be isolated from the reaction mixture according to ordinary methods, or easily purified by separating means such as recrystallization, distillation, chromatography or the like.
  • Alternatively, compound (8b) and R1-M (9b) (where M represents COOH) may be reacted in a solvent (for example, DMF, THF, toluene or methylene chloride) in the presence of a condensing agent and a base to obtain compound (11b). As condensing agents there may be mentioned 1,3-dicyclohexyl carbodiimide (DCC), 1-ethyl-3-[3-(dimethylamino)propyl] carbodiimide (EDC), tetramethylfluoroformamidium hexafluorophosphate (TFFH), O-(7-azabenzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (HATU) and the like. As bases there may be mentioned aromatic amines such as pyridine, lutidine and the like, and tertiary amines such as triethylamine, tripropylamine, tributylamine, cyclohexyldimethylamine, diisopropylethylamine, 4-dimethylaminopyridine, N,N-dimethylaniline, N-methylpiperidine, N-methylpyrrolidine, N-methylmorpholine and the like. The reaction may be conducted for 0.5 to 24 hours, and preferably 1-12 hours, at a temperature from -40 to 80°C and preferably -10 to 60°C. The product may be used either directly as the reaction solution or as a crude substance for the subsequent reaction, or it may be isolated from the reaction mixture according to ordinary methods, or easily purified by separating means such as recrystallization, distillation, chromatography or the like.
    Figure 00310001
    • [Step 1C]
  • Compound (1c) and compound (2c) may be used to obtain compound (3c) by the same method as Step 1B above.
    • [Step 2C]
  • Compound (1c) and compound (4c) may be used to obtain compound (5c) by the same method as Step 1B above.
    • [Step 3C]
  • The halogen atom (Hal) of compound (5c) may be converted to a cyano group by the same method described in Synthetic Communications, Vol.24, p.889, 1994, to obtain compound (3c). Specifically, compound (5c) may be reacted in a solvent (for example, DMF) in the presence of zinc cyanide and a palladium catalyst.
    • [Step 4C]
  • Compound (3c) may be used to obtain compound (6c) by the same method as Step 5B above.
    • [Step 5C]
  • Compound (7c) may be obtained from compound (6c) using the Sandmeyer method described in Synthetic Communications, Vol.22, (21), p.3067, 1992. Specifically, compound (6c) and a nitrous acid ester may be reacted in the presence of a copper halide. The product may be used as a crude substance for the subsequent reaction, or it may be isolated from the reaction mixture according to ordinary methods, or easily purified by separating means such as recrystallization, distillation, chromatography or the like.
    • [Step 6C]
  • Compound (7c) and compound (8c) may be used to obtain compound (9c) by the same method as Step 2B above.
  • After completion of the reaction, the reaction product may be purified by any desired ordinary treatment method, for example, by column chromatography using silica gel, an adsorbing resin or the like, or recrystallization from a suitable solvent. Purification and identification of the various compounds in each of the steps may be accomplished in a fully automatic manner by HPLC and MS, where purification by HPLC is conducted simultaneously with identification of the compounds based on detection of (M+1)+ by MS.
  • An amidino derivative of the invention, its pharmacologically acceptable salt or its solvate which is obtained in this manner may be used as a compound library. Further a set of 1 to 1 million amidino derivatives or their pharmacologically acceptable salts or their solvates may be used as a compound library.
  • Blood clotting factor VIIa inhibitors, anticoagulants and thrombosis therapeutic agents according to the invention will now be explained. The amidino derivatives of the invention and their pharmacologically acceptable salts and solvates inherently have serine protease inhibiting activity, and exhibit excellent inhibiting activity particularly against blood clotting factor VIIa. The amidino derivatives of the invention and their pharmacologically acceptable salts and solvates therefore function as blood clotting factor VIIa inhibitors (blood clotting factor VIIa enzyme activity inhibitors), and are hence useful as prophylactic and/or therapeutic agents for clinical conditions involving thrombogenesis in which extrinsic blood clotting mechanisms are implicated, i.e. as anticoagulants (particularly anticoagulants with inhibiting activity against blood clotting factor VIIa) or as thrombosis therapeutic agents.
  • As illnesses that may be prevented and/or treated by formulations according to the invention there may be mentioned thrombosis, deep venous thrombosis, pulmonary embolism, acute myocardial infarction, unstable angina, cerebral thrombosis, restenosis, arteriosclerosis and glomerulosclerosis.
  • The blood clotting factor VIIa inhibitors of the invention comprise the aforementioned amidino derivatives of the invention or their pharmacologically acceptable salts or solvates. The anticoagulants of the invention contain the aforementioned amidino derivatives of the invention or their pharmacologically acceptable salts or solvates as active ingredients. The thrombosis therapeutic agents of the invention also contain the aforementioned amidino derivatives of the invention or their pharmacologically acceptable salts or solvates as active ingredients.
  • The anticoagulants and thrombosis therapeutic agents of the invention may be formulated using ordinary formulation techniques, for use in the form of solid, semi-solid or liquid pharmaceutical formulations. Specifically, the active ingredient (an amidino derivative of the invention or its pharmacologically acceptable salt or solvate) may be combined with ordinary medicinally acceptable atoxic carriers to prepare any desired dosage form such as tablets, pellets, lozenges, capsules, granules, syrup, powder, suppository, cream, ointment, injection or the like. If necessary, adjuvants, stabilizers, thickeners, preservatives, osmotic regulating salts, buffering agents, coloring agents, aromas or the like may also be added to the formulation.
  • The content of the active ingredient in an anticoagulant or thrombosis therapeutic agent according to the invention is not particularly restricted, and may be appropriately selected depending on the dosage form. Also, two or more different active ingredients may be used in combination in an anticoagulant or thrombosis therapeutic agent of the invention.
  • A blood clotting factor VIIa inhibitor, anticoagulant or thrombosis therapeutic agent of the invention may be orally, enterally or intravenously administered, depending on the dosage form. The administered dose will differ considerably depending on the type of illness, severity of symptoms, patient age and gender, sensitivity to the drug agent and the like, but the daily dose for adults will generally be between about 0.03 mg and about 1000 mg, preferably between about 0.1 mg and about 500 mg and even more preferably between about 0.1 mg and about 100 mg. The dose may be administered at once or in divided doses per day, or at once or in divided doses every several days. In the case of injection, the dose will usually be from about 1 µg to about 3000 µg and preferably from about 3 µg to about 1000 µg per kg of body weight.
    • EXAMPLES
  • The present invention will now be explained in more specific detail through Production Examples and Examples, with the understanding that the invention is in no way limited to these examples. The symbol "↑" in the tables which follow indicates that the group is the same group as in the previous compound.
    • Production Example 1 t-Butyl 2-fluoro-5-nitrobenzoate
  • Figure 00360001
  • After dissolving 2-fluoro-5-nitrobenzoic acid (2.42 g) in toluene (30 ml), 1,1-di-tert-butoxytrimethylamine (10.6 g) was added dropwise over a period of 20 minutes while heating to reflux, and then the mixture was heated to reflux for another 30 minutes. After cooling to room temperature, washing was performed with saturated sodium bicarbonate solution and saturated saline, drying was performed over magnesium sulfate, and the solvent was distilled off. The residue was purified by silica gel chromatography (300 g, hexane:ethyl acetate = 5:1) to obtain 2.48 g of t-butyl 2-fluoro-5-nitrobenzoate.
    1H-NMR (CDCl3) δ : 1.61 (9H, s), 7.34 (1H, d d, J=8.8, 46.4Hz), 8.23-8.28 (1H, m), 8.65 (1H, dd, J=2.4, 7.2Hz).
    • Production Example 2-1 t-Butyl 2-(6-cyano-2-naphthyloxy)-5-nitrobenzoate
  • Figure 00370001
  • The compound (t-butyl 2-fluoro-5-nitrobenzoate) (1.74 g) obtained in a previous production example, 6-cyano-2-naphthol (1.86 g) and potassium carbonate (8.2 g) were heated to 50°C in N,N-dimethylformamide and reacted for 2 hours. After cooling to room temperature, water was added and extraction was performed with ethyl acetate. The ethyl acetate layer was washed with saturated saline and dried over magnesium sulfate, and the solvent was distilled off. The residue was purified by silica gel chromatography (300 g, hexane:ethyl acetate = 5:1) to obtain 3.23 g of t-butyl 2-(6-cyano-2-naphthyloxy)-5-nitrobenzoate.
    1H-NMR (CDCl3) δ : 1.38 (9H, s), 7.05 (1H, d, J=9.2Hz), 7.24 (1H, d, J=2.4Hz), 7.32 (1H, dd, J=2.4, 8.8Hz), 7.55 (1H, dd, J=1. 6, 8.4Hz), 7.72 (1H, d, J=8.4Hz), 7.89 (1H, d, J=8.8Hz), 8.16 (1H, s) , 8.25 (1H, dd, J=2.8, 8.8Hz).
    • Production Examples 2-2 to 2-7
  • Compounds represented by the following formula (Ia): HO-Ar-CN (where each Ar is the respective Ar in Table 1)
    were used to obtain compounds for Production Examples 2-2 to 2-7 by the same reaction as in Production Example 2-1.
    Figure 00390001
    • Production Example 2-2 t-Butyl 2-(4-cyanophenoxy)-5-nitrobenzoate
  • 1H-NMR (CDCl3) δ : 1.44 (9H, s) , 7.04 (2H, dd, J=2.0, 6.8Hz), 7.15 (1H, d, J=8.8Hz), 7.67 (2H, dd, J=2.0, 6.8Hz), 8.35 (1H, dd, J=2.8, 9.2Hz), 8.74 (1H, d, J=2.4).
    • Production Example 2-3 t-Butyl 2-(3-cyanophenoxy)-5-nitrobenzoate
  • 1H-NMR (CDCl3) δ : 1.48 (9H, s), 7.05 (1H, d, J=9.2Hz), 7.24-7.28 (2H, m), 7.47-7.5 3 (2H, m), 8.33 (1H, dd, J=2.8, 9.2Hz), 8.73 (1H, d, J=2.8Hz).
    • Production Example 2-4 t-Butyl 2-(4-cyano-1,1'-biphenyl-4'-yloxy)-5-nitrobenzoate
  • 1H-NMR (CDCl3) δ : 1.53 (9H, s), 7.05 (1H, d, J=9.2Hz), 7.15 (2H, dd, J=2.0, 6.8Hz), 7.62-7.75(6H, m), 8.27 (1H, dd, J=3.2, 9.2Hz), 8.70 (1H, d, J=2.8Hz).
    • Production Example 2-5 t-Butyl 2-[2-(4-cyanophenyl)ethoxy]-5-nitrobenzoate
  • 1H-NMR (CDCl3) δ : 1.59 (9H, s), 3.25 (2H, t, J=6.8Hz), 4.33 (2H, t, J=6.0Hz), 6.97 (1H, d, J=9.2Hz), 7.47 (2H, d, J=8.8Hz), 7.61 (2H, dd, J=1.6, 6.4Hz), 8.28 (1H, dd, J=2.8, 9.2Hz), 8.54 (1H, d, J=2.4Hz).
    • Production Example 2-6 3-bromo-4-(6-cyano-2-naphthyloxy) nitrobenzene
  • 1H-NMR (CDCl3) δ : 7.02 (1H, d, J=9.2Hz), 7.39 (1H, dd, J=2.4, 9.2Hz), 7.41 (1H, s), 7.65 (1H, dd, J=2.0, 8.8Hz), 7.83 (1H, d, J=8.4Hz), 7.99 (1H, d, J=8.4Hz), 8.17 (1H, dd, J=2.8, 9.2Hz), 8.26 (1H, s), 8.61 (1H, d, 2.4Hz).
    • Production Example 2-7 3-bromo-2-(4-cyanophenoxy)nitrobenzene
  • 1H-NMR (CDCl3) δ : 7.06-7.10 (3H, m), 7.71 (2H, dd, J=2.0, 6.8Hz), 8.20 (1H, dd, J=3.2, 9.6Hz), 8.59 (1H, d, J=2.8Hz).
    • Production Example 3-1 4-(6-cyano-2-naphthyloxy)-3-(2-formylphenyl)nitrobenzene
  • Figure 00410001
  • The compound (3-bromo-4-(6-cyano-2-naphthyloxy)nitrobenzene) (1 g) obtained in a previous production example, 2-formylphenyl boronic acid (610' mg) and tetrakis (triphenylphosphine) palladium (0) (150 mg) were heated to reflux and reacted for 6 hours in a mixture of 2 M sodium carbonate aqueous solution (10 ml), toluene (10 ml) and ethanol (10 ml). After cooling to room temperature, water was added and extraction was performed with ethyl acetate. The ethyl acetate layer was washed with saturated saline and dried over magnesium sulfate, and the solvent was distilled off. The residue was purified by silica gel chromatography (200 g, hexane:ethyl acetate = 4:1) to obtain 880 mg of 4-(6-cyano-2-naphthyloxy)-3-(2-formylphenyl) nitrobenzene.
    1H-NMR (CDCl3) δ : 7.02 (1H, d, J=9.2Hz), 7.24 (1H, dd, J=2.4, 9.2Hz), 7.35 (1H, d, J=2.4Hz), 7.43 (1H, d, J=7.6Hz), 7.56-7.63 (2H, m), 7.68 (1H, t, J=7.6Hz), 7.78 (1H, d, J=8.8Hz), 7.87 (1H, d, J=9.2Hz), 7.96 (1H, d, J=7.6Hz), 8.19 (1H, s), 8.27 (1H, dd, J=2.8, 8.8Hz), 8.34 (1H, d, J=2.8Hz), 10.04 (1H,s).
    • Production Examples 3-2 to 3-8
  • Compounds represented by the following formula (IIa) : (HO)2B-Ar2-CHO (where each Ar2 is the respective Ar2 in Table 2)
    were used to obtain compounds for Production Examples 3-2 to 3-8 by the same reaction as in Production Example 3-1.
    Figure 00430001
    • Production Example 3-2 2-(4-cyanophenoxy)-3-(2-formylphenyl) nitrobenzene
  • 1H-NMR (CDCl3) δ : 6.97 (1H, d, J=9.2Hz), 7.07 (1H, dd, J=0.8, 8.4Hz), 7.36 (1H, dd, J=1.2, 7.6Hz), 7.56-7.60 (3H, m), 7.65-7.69 (1H, m), 7.94 (1H, dd, J=1.6, 7.6Hz), 8.28-8.32 (2H, m), 9.96 (1H, s).
    • Production Example 3-3 4-(6-cyano-2-naphthyloxy)-3-(3-formylphenyl)nitrobenzene
  • 1H-NMR (CDCl3) δ : 7.26 (1H, d, J=8.8Hz), 7.53 (1H, dd, J=2.4, 9.2Hz), 7.67 (1H, t, J=7.6Hz), 7.70 (1H, d, J=2.4Hz), 7.77 (1H, dd, J=2.0, 8.8Hz), 7.91 (1H, dd, J=1.2, 7.6Hz), 8.00 (1H, s), 8.02 (1H, s), 8.13 (1H, d, J=8.8Hz), 8.18 (1H, s), 8.28 (1H, dd, J=2.8, 8.8Hz), 8.41 (1H, d, J=2.8Hz), 8.58 (1H, s), 10.04 (1H, s).
    • Production Example 3-4 2-(4-cyanophenoxy)-3-(3-formylphenyl) nitrobenzene
  • 1H-NMR (CDCl 3) δ : 7.02 (2H, dd, J=2.0, 6.8Hz), 7.15 (1H, d, J=8.8Hz), 7.59-7.63 (3H, m), 7.77-7.80 (3H, m), 7.89-7.91 (1H, m), 8.05-8.06 (1H, m), 8.27 (1H, dd, J=2.8, 9.2Hz), 8.42 (1H, d, J=2.8Hz), 10.05 (1H, s).
    • Production Example 3-5 4-(6-cyano-2-naphthyloxy)-3-(3-formyl-2-naphthyl)nitrobenzene
  • 1H-NMR (CDCl3) δ : 7.17 (1H, d, J=9.2Hz), 7.35 (1H, dd, J=2.0, 8.8Hz), 7.61 (1H, d, J=2.0Hz), 7.65-7.76 (3H, m), 7.98 (1H, d, J=8.8Hz), 8.04-8.09 (3H, m), 8.17 (1H, d, J=8.4Hz), 8.32 (1H, dd, J=2.8, 9.2Hz), 8.40 (1H, d, J=2.8Hz), 8.53 (1H, s), 8.59 (1H, s), 10.12 (1H, s) .
    • Production Example 3-6 4-(6-cyano-2-naphthyloxy)-3-(2-formylthiophen-3-yl)nitrobenzene
  • 1H-NMR (CDCl3) δ : 7.28 (1H, d, J=8.8Hz), 7.45 (1H, d, J=4.8Hz), 7.47 (1H, dd, J=2.4, 8.8Hz), 7.68 (1H, d, J=2.4Hz), 7.77 (1H, dd, J=1.6, 8.8Hz), 7.99 (1H, d, J=8.8Hz), 8.10-8.13 (2H, m), 8.33 (1H, dd, J=2.8, 9.2Hz), 8.45 (1H, d, J=3.2Hz), 8.57 (1H, s), 9.8 5 (1H, s).
    • Production Example 3-7 4-(6-cyano-2-naphthyloxy)-3-(4,5-dimethoxy-2-formylphenyl)nitrobenzene
  • 1H-NMR (CDCl3) δ : 3.93 (3H, s) , 3.96 (3H, s), 6.82 (1H, s), 7.06 (1H, d, J=9.2Hz), 7.2 3 (1H, dd, J=2.4, 9.2Hz), 7.35 (1H, d, J=2.4Hz), 7.47 (1H, s), 7.62 (1H, dd, J=1.6, 8.8Hz), 7.78 (1H, d, J=8.4Hz), 7.88 (1H, d, J=8.8Hz), 8.20 (1H, s), 8.27 (1H, dd, J=2.8, 9.2Hz), 8.36 (1H, d, J=2.8Hz), 9.87 (1H, s).
    • Production Example 3-8 4-(6-cyano-2-naphthyloxy)-3-(2-formyl-4-[2-methylpropoxy]phenyl)nitrobenzene
  • 1H-NMR (CDCl3) δ : 1.03 (6H, d, J=6.8Hz), 2.08 (1H, m), 3.79 (2H, d, J=6.8Hz), 7.03 (1H, d, J=9.2Hz), 7.18-7.24 (2H, m), 7.32 (1H, s), 7.34 (1H, d, J=6.0Hz), 7.45 (1H, d, J=2.8Hz), 7.61 (1H, dd, J=1.6, 8.8Hz), 7.77 (1H, d, J=8.8Hz), 7.88 (1H, d, J=9.2Hz), 8.19 (1H, s), 8.25 (1H, dd, J=3.2, 9.2Hz), 8.33 (1H, d, J=2.8Hz), 9.97 (1H, s).
    • Production Example 3-9 Methyl 2'-(6-cyano-2-naphthyloxy)-4-[(2-methylpropyl)carbamoyl]-5'-nitro-1,1'-biphenyl-2-carboxylate
  • Figure 00460001
  • The title compound was obtained by the same process as in Production Example 3-1, except that pinacol 4-[(2-methylpropyl)carbamoyl-3-methoxycarbonylphenylboronate was used instead of 2-formylphenylboronic acid.
    1H-NMR (CDCl3) δ : 0.99 (6H, d, J=6.8Hz), 1.87-1.95 (1H, m), 3.31 (2H, dd, J=6.8, 6.0Hz), 3.74 (3H, s) , 6.21 (1H, brs) 7.01 (1H, d, J=9.2Hz), 7.25-7.2 9 (1H, m), 7.36 (1H, d, J=2.4Hz), 7.49 (1H, d, J=8.4Hz), 7.60 (1H, dd, J=1.8, 8.6Hz), 7.77 (1H, d, J=8.8Hz), 7.88 (1H, d, J=9.2Hz), 8.02 (1H, dd, J=2.0, 8.0Hz), 8.20 (1H, s), 8.24 (1H, dd, J=2.8, 9.2Hz), 8.29 (1H, d, J=2.4Hz), 8.34 (1H, d, J=2.0Hz).
    • Production Example 4-1 t-Butyl 2'-(6-cyano-2-naphthyloxy)-5'-nitro-1,1'-biphenyl-2-carboxylate
  • Figure 00470001
  • The compound (4-(6-cyano-2-naphthyloxy)-3-(2-formylphenyl)nitrobenzene) (800 mg) obtained in a previous production example, 2-methyl-2-butene (1.4 ml) and sodium dihydrogen phosphate dihydrate (2 g) were dissolved in acetonitrile:water (2:1, 50 ml) and then sodium chlorate (1 g) was added. After reaction for one hour at room temperature, 1 N hydrochloric acid water was added and extraction was performed with ethyl acetate. The ethyl acetate layer was washed with 1N hydrochloric acid water and saturated saline and then dried over magnesium sulfate, and the solvent was distilled off to obtain 5'-nitro-2'-(6-cyano-2-naphthyloxy) -1,1'-biphenyl-2-carboxylic acid (800 mg). This was dissolved in toluene without purification and subjected to the same process as in Production Example 1 to obtain t-butyl 2'-(6-cyano-2-naphthyloxy)-5'-nitro-1,1'-biphenyl-2-carboxylate.
    1H-NMR (CDCl3) δ : 1.38 (9H, s) , 7.08 (1H, dd, J=0.8, 9.2Hz), 7.25 (1H, dd, J=2.4, 8.4 Hz), 7.29-7.33 (2H, m), 7.41 (1H, t, J=7.6Hz), 7.49 (1H, t, J=7.6Hz), 7.57 (1H, d, J=7.6Hz), 7.72 (1H, d, J=8.4Hz), 7.81 (1H, d, J=8.8Hz), 7.93 (1H, d, J=7.6Hz), 8.15 (1H, s), 8.24 (1 H, ddd, J=0.8, 2.8, 8.8Hz), 8.29 (1H, dd, J=0.8, 2.8Hz).
    • Production Examples 4-2 to 4-8
  • The compounds {2-(4-cyanophenoxy)-3-(2-formylphenyl) nitrobenzene, 4-(6-cyano-2-naphthyloxy)-3-(3-formylphenyl) nitrobenzene, 2-(4-cyanophenoxy)-3-(3-formylphenyl)nitrobenzene, 4-(6-cyano-2-naphthyloxy)-3-(3-formyl-2-naphthyl)nitrobenzene, 4-(6-cyano-2-naphthyloxy)-3-(2-formylthiophen-3-yl)nitrobenzene, 4-(6-cyano-2-naphthyloxy)-3-(4,5-dimethoxy-2-formylphenyl) nitrobenzene and 4-(6-cyano-2-naphthyloxy)-3-(2-formyl-4-[2-methylpropoxy]phenyl)nitrobenzene} obtained in previous production examples, were used for the same reaction as in Production Example 4-1 to obtain compounds for Production Examples 4-2 to 4-8, respectively.
    Figure 00490001
    • Production Example 4-2 t-Butyl 2'-(4-cyanophenoxy)-5'-nitro-1,1'-biphenyl-2-carboxylate
  • 1H-NMR (CDCl3) δ : 1.30 (9H, s), 6.87 (1H, d, J=8.8Hz), 7.05 (1H, dd, J=0.8, 8.0Hz), 7.17-7.20 (1H, m), 7.33-7.47 (3H, m), 7.85 (1H, dd, J=1.6, 7.6Hz), 8.1 8-8.2 1 (2H, m).
    • Production Example 4-3 t-Butyl 2'-(6-cyano-2-naphthyloxy)-5'-nitro-1,1'-biphenyl-3-carboxylate
  • 1H-NMR (CDCl3) δ : 1.55 (9H, d), 7.12 (1H, d, J=8.8Hz), 7.32 (1H, dd, J=2.4, 9.2Hz), 7.36 (1H, d, J=2.4Hz), 7.48 (1H, t, J=7.6Hz), 7.61 (1H, dd, J=1.6, 8.8Hz), 7.73-7.79 (2H, m), 7.92 (1H, d, J=9.2Hz), 7.98-8.01 (1H, m), 8.20-8.24 (3H, m), 8.43 (1H, d, J=2.8Hz).
    • Production Example 4-4 t-Butyl 2'-(4-cyanophenoxy)-5'-nitro-1,1'-biphenyl-3-carboxylate
  • 1H-NMR (CDCl3) δ : 1.50 (9H, s), 6.93-6.97 (2H, m), 7.08 (1H, d, J=9.2Hz), 7.38-7.4 2 (1H, m), 7.53-7.61 (3H, m), 7.92-7.94 (1H, m), 8.05-8.06 (1H, m), 8.17-8.20 (1H, m), 8.35 (1H, d, J=2.8Hz).
    • Production Example 4-5 t-Butyl 3-[2-(6-cyano-2-naphthyloxy)-5-nitrophenyl]-naphthalene-2-carboxylate
  • 1H-NMR (CDCl3) δ : 1.43 (9H, s), 7.09 (1H, d, J=9.2Hz), 7.24-7.27 (1H, m), 7.3 2 (1H, d, J=2.4Hz), 7.53-7.60 (3H, m), 7.70 (1H, d, J=8.4Hz), 7.79 (1H, d, J=8.8Hz), 7.80 (1H, s), 7.84 (1H, d, J=7.6Hz), 7.93 (1H, d, J=7.6Hz), 8.12 (1H, s), 8.26 (1H, dd, J=2.8, 9.2Hz), 8.43 (1H, d, J=2.4Hz), 8.48 (1H, s) .
    • Production Example 4-6 t-Butyl 3-[2-(6-cyano-2-naphthyloxy)-5-nitrophenyl]-thiophene-2-carboxylate
  • 1H-NMR (CDCl3) δ : 1.41 (9H, s), 7.04-7.07 (2H, m), 7.32 (1H, dd, J=2.4, 8.8Hz), 7.37 (1H, d, J=2.0Hz), 7.46 (1H, dd, J=0.8, 5.2Hz), 7.59 (1H, dd, J=0.8, 8.4Hz), 7.75 (1H, d, J=8.4Hz), 7.86 (1H, dd, J=8.8H z), 8.19 (1H, s), 8.20-8.23 (1H, m), 8.32 (1H, d, J=2.4Hz).
    • Production Example 4-7 t-Butyl 2'-(6-cyano-2-naphthyloxy)-4,5-dimethoxy-5'-nitro-1,1'-biphenyl-2-carboxylate
  • 1H-NMR (CDCl3) δ : 1.34 (9H, s), 3.84 (3H, s), 3.91 (3H, s), 6.71 (1H, s), 7.08 (1H, d, J=8.8Hz), 7.24 (1H, d, J=2.8Hz), 7.30 (1H, d, J=2.8Hz), 7.48 (1H, s) , 7.58 (1H, dd, J=1.6, 8.4Hz), 7.72 (1H, d, J=8.8Hz), 7.82 (1H, d, J=8.8Hz), 8.17 (1H, s), 8.23 (1H, dd, J=2.8, 8.8Hz), 8.27 (1H, d, J=3.2Hz).
    • Production Example 4-8 t-Butyl 2'-(6-cyano-2-naphthyloxy)-4-(2-methylpropoxy)-5'-nitro-1,1'-biphenyl-2-carboxylate
  • 1H-NMR (CDCl3) δ : 1.01 (6H, d, J=6.8Hz), 1.36 (9H, s), 2.06 (1H, m), 3.73 (2H, d, J=6.8Hz), 6.99 (1H, dd, J=2.8, 8.8Hz), 7.08 (1H, d, J=8.8Hz), 7.20-7.27 (3H, m), 7.43 (1H, d, J=2.8Hz), 7.57 (1H, dd, J=1.6, 8.8Hz), 7.72 (1H, d, J=8.8Hz), 7.81 (1H, d, J=9.2Hz), 8.15 (1H, s), 8.21 (1H, dd, J=2.8, 8.8Hz), 8.27 (1H, d, J=2.8Hz).
    • Production Example 5-1 t-Butyl 5-amino-2-(6-cyano-2-naphthyloxy)benzoate
  • Figure 00520001
  • The compound (t-butyl 2-(6-cyano-2-naphthyloxy)-5-nitrobenzoate) (760 mg) obtained in a previous production example, iron powder (718 mg) and ammonium chloride (73 mg) were heated to reflux in ethanol:water (3:1, 30 ml) and reacted for 30 minutes. The insoluble portion was immediately filtered using celite, and the filtrate was concentrated. A saturated sodium bicarbonate solution was added to the obtained residue, and extraction was performed with ethyl acetate. The ethyl acetate layer was washed with saturated saline and then dried over magnesium sulfate, and the solvent was distilled off to obtain 736 mg of t-butyl 5-amino-2-(6-cyano-2-naphthyloxy)benzoate.
    1H-NMR (d6 -DMSO) δ : 1.12 (9H, s), 5.35 (1H, b rd), 6.79 (1H, dd, J=2.4, 8.4Hz), 6.92 (1H, d, J=8.8Hz), 6.99 (1H, d, J=2.8Hz), 7.01 (1H, d, J=2.8Hz), 7.35 (1H, dd, J=2.4, 8.8Hz), 7.64 (1H, dd, J=1.6, 8.8Hz), 7.89 (1H, d, J=8.4Hz), 8.01 (1H, d, J=9.2Hz), 8.46 (1H, s).
    • Production Examples 5-2 to 5-13
  • The compounds {t-butyl 2-(6-cyano-2-naphthyloxy)-5-nitrobenzoate, t-butyl 2-(4-cyanophenoxy)-5-nitrobenzoate, t-butyl 2-(3-cyanophenoxy)-5-nitrobenzoate, t-butyl 2-(4-cyano-1,1'-biphenyl-4'-yloxy)-5-nitrobenzoate, t-butyl 2-[2-(4-cyanophenyl)ethoxy]-5-nitrobenzoate, t-butyl 2'-(6-cyano-2-naphthyloxy)-5'-nitro-1,1'-biphenyl-2-carboxylate, t-butyl 2'-(4-cyanophenoxy)-5'-nitro-1,1'-biphenyl-2-carboxylate, t-butyl 2'-(6-cyano-2-naphthyloxy)-5'-nitro-1,1'-biphenyl-3-carboxylate, t-butyl 2'-(4-cyanophenoxy)-5'-nitro-1,1'-biphenyl-3-carboxylate, t-butyl 3-[2-(6-cyano-2-naphthyloxy)-5-nitrophenyl]-naphthalene-2-carboxylate, t-butyl 3-[2-(6-cyano-2-naphthyloxy)-5-nitrophenyl]-thiophene-2-carboxylate, t-butyl 2'-(6-cyano-2-naphthyloxy)-4,5-dimethoxy-5'-nitro-1,1'-biphenyl-2-carboxylate, t-butyl 2'-(6-cyano-2-naphthyloxy)-4-(2-methylpropoxy)-5'-nitro-1,1'-biphenyl-2-carboxylate and methyl 2'-(6-cyano-2-naphthyloxy)-4-[(2-methylpropyl)carbamoyl]-5'-nitro-1,1'-biphenyl-2-carboxylate} obtained in the previous production examples, were used for the same reaction as in Production Example 5-1 to obtain compounds for Production Examples 5-2 to 5-13, respectively.
    Figure 00550001
    • Production Example 5-2 t-Butyl 5-amino-2-(4-cyanophenoxy)benzoate
  • 1H-NMR (d6-DMSO) δ : 1.19 (9H, s ) , 5.38 (1H, brd) , 6.77 (1H, dd, J=3.2, 8.8Hz), 6.85-6.88 (3H, m), 6.97(1H, d, J=2.8Hz), 7.73 (2H, dd, J=2.0, 6.8Hz).
    • Production Example 5-3 t-Butyl 5-amino-2-(3-cyanophenoxy)benzoate
  • 1H-NMR (d6-DMSO) δ : 1.20 (9H, s), 5.35 (1H, brd, J=7.6Hz), 6.76 (1H, dd, J=3.2, 8.8Hz), 6.68(1H, d, J=8.8Hz), 6.96 (1H, d, J=2.8Hz), 7.05 (1H, m), 7.13 (1H, brs), 7, 39-7.48 (2H, m).
    • Production Example 5-4 t-Butyl 5-amino-2-(4-cyano-1,1'-biphenyl-4'-yloxy)benzoate
  • 1H-NMR (d6-DMSO) δ : 1.23 (9H, s), 5.29 (1H, brd, J=7.6Hz), 6.76 (1H, dd, J=2.8, 8.4Hz) , 6.82-6.87 (3H, m), 6.95 (1H, d, J=2.8Hz), 7.68 (2H, dd, J=2.0, 6.8Hz), 7.78-7.87 (4H, m).
    • Production Example 5-5 t-Butyl 5-amino-2-[2-(4-cyanophenyl) ethoxy]benzoate
  • 1H-NMR (d6-DMSO) δ : 1.42 (9H, s), 3.04 (2H, t, J=6.4Hz), 4.07 (2H, t, J=6.8Hz), 4.81 (1H, brd, J=8.8Hz), 6.61 (1H, dd, J=2.4, 8.4Hz), 6.71 (1H, d, J=3.2Hz), 6.76 (1H, d, J=8.8Hz), 7.50 (2H, d, J=8.0Hz), 7.73 (2H, dd, J=2.0, 6.8Hz) .
    • Production Example 5-6 t-Butyl 5'-amino-2'-(6-cyano-2-naphthyloxy)-1,1'-biphenyl-2-carboxylate
  • 1H-NMR (d6-DMSO) δ : 1.32 (9H, s), 5.15 (1H, brd, J=8Hz), 6.55 (1H, d, J=2.8Hz), 6.64 (1H, dd, J=2.8, 8.8Hz), 6.88 (1H, d, J=6Hz), 7.01 (1H, d, J=2.4Hz) 7.09 (1H, dd, J=2.4, 9.2Hz) , 7.19-7.23(2H, m), 7.31 (1H, t, J=7.2Hz), 7.56(1H, d, J=7.6Hz), 7.59 (1H, d, J=8.4Hz), 7.78 (1H, d, J=8.8H z), 7.83 (1H, d, J=9.2Hz), 8.35 (1H, s).
    • Production Example 5-7 t-Butyl 5'-amino-2'-(4-cyanophenoxy)-1,1'-biphenyl-2-carboxylate
  • 1H-NMR ( d 6-DMSO) δ : 1.30 (9H, s), 5.18 (1H, brs), 6.52 (1H, d, J=2.4Hz), 6.61 (1H, dd, J=2.8, 8.8Hz), 6.72-6.75 (2H, m), 6.83 (1H, d, J=8.4Hz), 7.18 (1H, dd, J=0.8, 7.2Hz), 7.25-7.29 (1H, m), 7.36-7.40 (1H, m) , 7.54-7.59 (2H, m) .
    • Production Example 5-8 t-Butyl 5'-amino-2'-(6-cyano-2-naphthyloxy)-1,1'-biphenyl-3-carboxylate
  • 1H-NMR (d6-DMSO) δ : 1.33 (9H, s), 5.26 (1H, brd, J=8.4Hz), 6.68 (1H, dd, J=2.8, 8.8Hz), 6.73 (1H, d, J=2.4Hz), 6.96 (1H, d, J=8.8Hz), 7.14 (1H, d, J=2.4Hz), 7.27 (1H, dd, J=2.8, 9.2Hz), 7.42 (1H, t, J=7.6H z), 7.62-7.65 (2H, m), 7.70-7.73 (1H, m), 7.87-7.91 (2H, m), 7.95 (1H, d, J=8.8Hz), 8.44 (1H, s) .
    • Production Example 5-9 t-Butyl 5'-amino-2'-(4-cyanophenoxy)-1,1'-biphenyl-3-carboxylate
  • 1H-NMR (d6-DMSO) δ : 1.45 (9H, s), 5.28 (1H, brd, J=8.0Hz), 6.66 (1H, dd, J=2.8, 8.4Hz) , 6.70 (1H, d, J=2.4Hz), 6.86-6.91 (3H, m), 7.44 (1H, t, J=7.6Hz), 7.59-7.61 (1H, m), 7.68 (2H, d, J=8.8Hz), 7.76 (1H, dd, J=1.2, 8.0Hz), 7.82 (1H, s).
    • Production Example 5-10 t-Butyl 3-[5-amino-2-(6-cyano-2-naphthyloxy)phenyl]-naphthalene-2-carboxylate
  • 1H-NMR (d6-DMSO) δ : 1.38 (9H, s), 5.19 (1H, brd, J=8.8Hz), 6.68 (1H, dd, J=2.4, 8.4Hz), 6.73 (1H, d, J=2.8Hz), 6.91 (1H, d, J=8.8Hz), 7.01 (1H, d, J=2.0Hz), 7.09 (1H, dd, J=2.4, 9.2Hz), 7.43-7.50 (2H, m), 7.55 (1H, dd, J=1.2, 8.4Hz) 7.74-7.81 (3H, m), 7.9 2 (1H, d, J=8.8Hz), 8.21 (1H, s), 8.2 8 (1H, s).
    • Production Example 5-11 t-Butyl 3-[5-amino-2-(6-cyano-2-naphthyloxy)phenyl]-thiophene-2-carboxylate
  • 1H-NMR (d6-DMSO) δ : 1.34 (9H, s), 5.15 (1H, brd, J=8.4Hz), 6.56 (1H, d, J=2.8Hz), 6. 64 (1H, dd, J=2.8, 8.4), 6.90 (1H, d, J=8. 8Hz), 6.95 (1H, d, J=4.8Hz), 7.03 (1H, d, J=2.4Hz), 7.14 (1H, dd, J=2.8, 9.2Hz), 7.58 (1H, d, J=4.8Hz), 7.62 (1H, dd, J=1.6, 8.8Hz), 7.78 (1H, d, J=8.8Hz), 7.87 (1H, d, J=9.2Hz), 8.39 (1H, s) .
    • Production Example 5-12 t-Butyl 5'-amino-2'-(6-cyano-2-naphthyloxy)-4,5-dimethoxy-1,1'-biphenyl-2-carboxylate
  • 1H-NMR (d6-DMSO) δ : 1.28 (9H, s), 3.47 (3H, s), 3.66 (3H, s), 5.11 (1H, brs), 6.52 (1H, d, J=2.4Hz), 6.62 (1H, dd, J=2.4, 8.4H z), 6.73 (1H, s), 6.87 (1H, d, J=8.8Hz), 7.08 (1H, d, J=2.8Hz), 7.12 (1H, s), 7.14 (1H, dd, J=2.4, 9.2Hz), 7.61 (1H, dd, J=1.6, 8.4Hz), 7.83 (2H, t, J=8.8Hz), 8.37 (1H, s).
    • Production Example 5-13 t-Butyl 5'-amino-2'-(6-cyano-2-naphthyloxy)-4-(2-methylpropoxy)-1,1'-biphenyl-2-carboxylate
  • 1H-NMR (d6-DMSO) δ : 0.87 (6H, d, J=6.8Hz), 1.32 (9H, s), 1.86 (1H, m), 3.63 (2H, d, J=6.8Hz), 5.11 (1H, brs), 6.52 (1H, d, J=2.8Hz), 6.61 (1H, dd, J=3.2, 8.4Hz), 6.86 (1H, d, J=8.4Hz), 6.88 (1H, dd, J=2.8, 8.4Hz), 7.00 (1H, d, J=2.4Hz), 7.05 (1H, d, J=2.8Hz), 7.11 (1H, dd, J=2.4, 8.8Hz), 7.13 (1H, d, J=8.4Hz), 7.60 (1H, dd, J=1.6, 8.4Hz), 7.79 (1H, d, J=8.4Hz), 7.84 (1H, d, J=9.2Hz), 8.36 (1H, s).
    • Production Example 5-14 Methyl 5'-amino-2'-(6-cyano-2-naphthyloxy)-4-[(2-methylpropyl)carbamoyl]-1,1'-biphenyl-2-carboxylate
  • 1H-NMR (CDCl3 + d6 -DMSO) δ : 0.96 (6H, d, J=7.2Hz), 1.54-1.62 (1H, m), 2.84-2.87 (2H, m) , 3.44 (3H, s), 6.44 (1H, d, J=2.8Hz), 6.47 (1H, dd, J=2.4, 8.4Hz), 6.62 (1H, d, J=8.4Hz), 6.67 (1H, d, J=2.8Hz), 6.83 (1H, dd, J=2.6, 9.0Hz), 7.09 (1H, d, J=8.0Hz), 7.18 (1H, dd, J=1.4, 8.6Hz), 7.32 (1H, d, J=8.8Hz), 7.40 (1H, d, J=8.8Hz), 7.47 (1H, brs), 7.58 (1 H, dd, J=2.0, 8.4H z), 7.79 (1H, s), 7.91 (1H, d, J=1.6Hz).
    • Production Example 6-1 2-(6-bromo-2-naphthyloxy)-4-nitrobenzonitrile
  • Figure 00600001
  • After heating to reflux 2-fluoro-4-nitrobenzonitrile (1 g), 6-bromo-2-naphthol (1.41 g), potassium fluoride/alumina (0.7 g) and 18-crown-6 (0.16 g) in acetonitrile, the mixture was reacted for 12 hours. It was then cooled to room temperature, the insoluble portion was filtered using celite, the ethyl acetate layer was washed with water and saturated saline and then dried over magnesium sulfate, and the solvent was distilled off. Methanol was added to the residue for crystallization to obtain 1.43 g of 2-(6-bromo-2-naphthyloxy)-4-nitrobenzonitrile.
    1H-NMR (CDCl3) δ : 7.31 (1H, dd, J=2.4, 8.8Hz), 7.54 (1H, d, J=2.0Hz), 7.62-7.70 (3H, m), 7.88 (1H, s), 7.90 (1H, s), 8.01 (1H, dd, J=2.0, 8.4Hz), 8.08 (1H, s).
    • Production Example 6-2 t-Butyl 2-(6-bromo-2-naphthyloxy)-4-nitrobenzoate
  • Figure 00610001
  • The compound (2-(6-bromo-2-naphthyloxy)-4-nitrobenzonitrile) (1 g) obtained in a previous production example was dissolved in acetic acid (20 ml) and then concentrated sulfuric acid (5 ml) was slowly added. After heating to reflux for 12 hours, the mixture was cooled to room temperature and ice water was added to obtain a precipitate. This was dissolved in toluene without purification and subjected to the same process as in Production Example 1 to obtain t-butyl 2-(6-bromo-2-naphthyloxy)-4-nitrobenzoate.
    1H-NMR (CDCl3) δ : 1.41 (9H, s), 7.20 (1H, d, J=2.8Hz), 7.28 (1H, dd, J=2.8, 9.2Hz), 7.53-7.59 (2H, m), 7.78-7.8 4 (2H, m), 7.96-8.06 (3H, m).
    • Production Example 6-3 t-Butyl 2-(6-cyano-2-naphthyloxy)-4-nitrobenzoate
  • Figure 00620001
  • The compound (t-butyl 2-(6-bromo-2-naphthyloxy)-4-nitrobenzoate) (1.78 g) obtained in a previous production example, zinc cyanide (0.33 g) and tetrakis(triphenylphosphine) palladium (0) (690 mg) were reacted for 12 hours at 100°C in N,N-dimethylformamide. After cooling to room temperature, toluene and ethyl acetate were added, the organic layer was washed with 2 N ammonia water and saturated saline and dried over magnesium sulfate, and the solvent was distilled off. The residue was purified by silica gel chromatography (300 g, hexane:ethyl acetate = 6:1) to obtain 1.14 g of t-butyl 2-(6-cyano-2-naphthyloxy)-4-nitrobenzoate.
    1H-NMR (CDCl3) δ : 1.37 (9H, s), 7.18 (1H, d, J=2.8Hz), 7.39 (1H, dd, J=2.4, 8.8Hz), 7.60 (1H, dd, J=1.6, 8.4Hz), 7.75 (1H, d, J=8.4Hz), 7.92 (1H, d, J=2.4Hz), 7.94 (1H, d, J=9.2Hz), 8.03 (1H, d, J=8.8Hz), 8.12 (1H, dd, J=2.0, 8.4Hz), 8.22 (1H, s)
    • Production Example 6-4 t-Butyl 4-amino-2-(6-cyano-2-naphthyloxy) benzoate
  • Figure 00630001
  • The compound (t-butyl 2-(6-cyano-2-naphthyloxy)-4-nitrobenzoate) obtained in a previous production example was treated by the same process as in Production Example 5-1 to obtain t-butyl 4-amino-2-(6-cyano-2-naphthyloxy)benzoate.
    1H-NMR (d6-DMSO) δ : 1.63 (9H, s), 6.05 (1H, d, J=4.8Hz), 6.23 (1H, d, J=2.4Hz), 6.49 (1H, dd, J=2.4, 8.8Hz), 7.14 (1H, d, J=2.8Hz), 7.37 (1H, dd, J=2.4, 9.2Hz), 7.62 (1H, d, J=8.4Hz), 7.67 (1H, dd, J=1.6, 8.8Hz), 7.94 (1H, d, J=8.8Hz), 8.05 (1H, d, J=9.2Hz), 8.49 (1H, s).
    • Production Examples 7-1 and 7-2
  • Compounds (Ia) represented by the formula HO-Ar-CN (wherein Ar represents the Ar groups in the following table) were used for reaction in the same manner as Production Example 2-1 to obtain compounds for Production Examples 7-1 and 7-2.
    Figure 00640001
    • Production Example 7-1 3-(6-cyano-2-naphthyloxy)-4-nitrotoluene
  • 1H-NMR (CDCl3) δ : 2.41 (3H, s), 6.97-6.98 (1H, m), 7.14-7.17 (1H, m), 7.23 (1H, d, J=2.8Hz), 7.39 (1H, dd, J=2.8, 8.8Hz), 7.59 (1H, dd, J=1.6, 8.6Hz), 7.76 (1H, d, J=8.8Hz), 7.92 (1H, d, J=8.8Hz), 7.99 (1H, d, J=8.4Hz), 8.21 (1H, d, J=0.8Hz).
    • Production Example 7-2 3-(4-cyanophenoxy)-4-nitrotoluene
  • 1H-NMR (CDCl3) δ : 2.44 (3H, s), 6.98-7.02 (3H, m), 7.17-7.19 (1H, m), 7.63 (2H, dd, J=2.0, 6.8Hz), 7.99 (1H, d, J=8.4Hz).
    • Production Examples 8-1 and 8-2
  • The two compounds obtained above, (3-(6-cyano-2-naphthyloxy)-4-nitrotoluene and 3-(4-cyanophenoxy)-4-nitrotoluene) were used for the same reaction as in Production Example 5-1 to obtain compounds for Production Examples 8-1 and 8-2, respectively.
    Figure 00660001
    • Production Example 8-1 4-amino-3-(6-cyano-2-naphthyloxy)toluene
  • 1H-NMR (CDC 1 3) δ : 2.25 (3H, s), 3.61 (2H, brs), 6.94-6.98 (2H, m), 7.08 (1H, d, J=2.4Hz), 7.37 (1H, dd, J=2.4, 8.8Hz), 7.54-7.5 8 (2H, m), 7.7 2 (1H, d, J=8.8Hz), 7.88 (1H, d, J=8.8Hz), 8.19 (1H, d, J=0.8H z)
    • Production Example 8-2 4-amino-3-(4-cyanophenoxy)toluene
  • 1H-NMR (CDCl3) δ : 2.24 (3H, s), 6.74-6.75 (2H, m) 6.77-6.90 (1 H, m), 6.98 (2H, dd, J=2.0, 6.8Hz), 7.58 (2H, dd, J=2.0, 6.8Hz).
    • Production Example 9-1 4-bromo-3-(6-cyano-2-naphthyloxy)toluene
  • Figure 00670001
  • After adding t-butyl nitrite (90 mL) and copper (II) bromide (142 mg) to a mixture of the compound (4-amino-3-(6-cyano-2-naphthyloxy)toluene) (760 mg) obtained above and 5 mL of acetonitrile at a room temperature, the mixture was stirred for one hour at 50°C. The reaction solution was returned to room temperature, water and ammonia water were added, and extraction was performed with ethyl acetate. The organic layer was washed with saturated saline, dried over anhydrous magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel chromatography (hexane:ethyl acetate = 20:1) to obtain 200 mg of the corresponding bromo compound (93% yield).
    1H-NMR (CDCl3) δ : 2.25 (3H, s) , 3.61 (2H, brs) , 6.79-6.81 (2H, m), 6.89-6.92 (1H, m), 7.15 (1H, d, J=2.8Hz), 7.39 (1H, dd, J=8.8, 2.8Hz), 7.54 (1H, dd, J=8.4, 1.6Hz), 7.71 (1H, d, J=8.8Hz), 7.86 (1H, d, J=8.8Hz), 8.17 (1H, s).
    • Production Example 9-2 4-bromo-3-(4-cyanophenoxy)toluene
  • Figure 00680001
  • The compound (4-amino-3-(4-cyanophenoxy)toluene) obtained above was used for the same reaction as in Production Example 9-1 to obtain the title compound. 1H-NMR (CDCl3) δ : 2.32 (3H, s), 6.91-6.98 (4H, m), 7.52 (1H, d, J=8.0Hz), 7.58-7.62 (2H, m).
    • Production Examples 10-1 and 10-2
  • The two compounds obtained above, (4-bromo-3-(6-cyano-2-naphthyloxy)toluene and 4-bromo-3-(4-cyanophenoxy) toluene) were used for the same reaction as in Production Example 3-9 to obtain compounds for Production Examples 10-1 and 10-2, respectively.
    Figure 00690001
    • Production Example 10-1 Methyl 2'-(6-cyano-2-naphthyloxy)-4-(2-methylpropylcarbamoyl)-4'-methyl-1,1'-biphenyl-2-carboxylate
  • 1H-NMR (CDCl3) δ : 0.95 (6H, d, J=6.4Hz), 1.83-1.95 (1H, m), 2.40 (3H, s ) , 3.22-3.30 (2H, m), 3.73 (3H, s), 6.92 (1H, s), 7.02 (1H, d, J=8.8Hz), 7.06 (1H, d, J=2.0Hz), 7.13-7.20 (2H, m), 7.29 (1H, d, J=7.6Hz), 7.42 (1H, d, J=8.0Hz), 7.50 (1H, d d, J=8.8, 1.6Hz), 7.64 (1H, d, J=8.4Hz), 7.71 (1H, d, J=8.8Hz), 7.85 (1H, d, J=8.2, 2.0Hz), 8.09 (1H, s), 8.12 (1H, d, J=2.0Hz).
    • Production Example 10-2 Methyl 2'-(4-cyanophenoxy)-4-(2-methylpropylcarbamoyl)-4'-methyl-1,1'-biphenyl-2-carboxylate
  • 1H-NMR (CDCl3) δ : 0.97 (6H, d, J=6.8Hz), 1.87-1.95 (1H, m), 3.28 (2H, dd, J=6.8, 6.0Hz), 3.72 (3H, s), 6.80-6.83 (2H, m), 6.88 (1H, d, J=1.2Hz), 7.15 (1H, dd, J=7.2, 1.2Hz), 7.26 (1H, d, J=7.2Hz), 7.36 (1H, d, J=8.0Hz), 7.42-7.46 (2H, m), 7.88 (1H, dd, J=8.0, 1.2Hz), 8.14 (1H, d, J=1.2Hz).
    • Example 1-1 2-(6-amidino-2-naphthyloxy)-5-methanesulfonyl-aminobenzoic acid trifluoroacetate
  • Figure 00700001
  • Methanesulfonyl chloride (8 mg) was added to a pyridine solution containing the compound (t-butyl 5-amino-2-(6-cyano-2-naphthyloxy)benzoate) (20 mg) obtained in a previous production example, and reaction was conducted at 50°C for 1 hour. The reaction solution was concentrated, a saturated sodium bicarbonate solution was added to the residue, and extraction was performed with ethyl acetate. After freezing the aqueous layer with ethanol-dry ice, the ethyl acetate layer was decanted and the solvent was distilled off. Hydroxyamine hydrochloride (14 mg) and potassium carbonate (15 mg) were added to the obtained residue, and reaction was conducted for 12 hours at 60°C in ethanol:water (2:1, 1 ml). After cooling to room temperature, the reaction solution was concentrated, saturated saline was added to the residue, and extraction was performed with ethyl acetate. After freezing the aqueous layer with ethanol-dry ice, the ethyl acetate layer was decanted and the solvent was distilled off. The obtained residue was dissolved in acetic acid (1 ml), acetic anhydride (8 mg) was added, and reaction was conducted at room temperature for 15 minutes. Next, 10% palladium-carbon (1 mg) was added thereto and catalytic reduction was carried out for 6 hours under ordinary pressure. The catalyst was then filtered off using celite, and the filtrate was concentrated. The residue was dissolved in trifluoroacetic acid:dichloromethane (1:1, 1 ml) and reaction was conducted at room temperature for 90 minutes. The solvent was distilled off and the residue was purified by LC-MS (from 1% acetonitrile/water (0.1% trifluoroacetic acid) to 80% acetonitrile/water (0.1% trifluoroacetic acid), flow rate: 20 ml/min) to obtain 11.4 mg of 2-(6-amidino-2-naphthyloxy)-5-[(methylsulfonyl)amino]benzoic acid trifluoroacetate.
    RT = 6.68 min, ESI-MS (m/z): 400.17 (M+1)+.
    • Examples 1-2 to 1-69
  • The compounds {t-butyl 5-amino-2-(6-cyano-2-naphthyloxy) benzoate, t-butyl 5-amino-2-(4-cyanophenoxy)benzoate, t-butyl 5-amino-2-(3-cyanophenoxy)benzoate, t-butyl 5-amino-2-(4-cyano-1,1'-biphenyl-4'-yloxy)benzoate, t-butyl 5-amino-2-[2-(4-cyanophenyl)ethoxy]benzoate, t-butyl 5'-amino-2'-(6-cyano-2-naphthyloxy)-1,1'-biphenyl-2-carboxylate, t-butyl 5'-amino-2'-(4-cyanophenoxy)-1,1'-biphenyl-2-carboxylate, t-butyl 5'-amino-2'-(6-cyano-2-naphthyloxy)-1,1'-biphenyl-3-carboxylate, t-butyl 5'-amino-2'-(4-cyanophenoxy)-1,1'-biphenyl-3-carboxylate, t-butyl 3-[5-amino-2-(6-cyano-2-naphthyloxy)phenyl]-naphthalene-2-carboxylate, t-butyl 3-[5-amino-2-(6-cyano-2-naphthyloxy)phenyl]-thiophene-2-carboxylate, t-butyl 5'-amino-2'-(6-cyano-2-naphthyloxy)-4,5-dimethoxy-1,1'-biphenyl-2-carboxylate and t-butyl 5'-amino-2'-(6-cyano-2-naphthyloxy)-4-(2-methylpropoxy)-1,1'-biphenyl-2-carboxylate} obtained in the previous production examples, and sulfonylchloride derivatives represented by the following formula (Ib) : R1-SO2-Cl (where R1 represents the respective R1 groups in Tables 8 to 12) were used for reaction in the same manner as in Example 1-1 to obtain compounds for Examples 1-2 to 1-69, respectively.
    Figure 00730001
    Figure 00740001
    Figure 00750001
    Figure 00760001
    Figure 00770001
    Example Compound name
    1-2 2-(6-amidino-2-naphthyloxy)-5-(4-methoxybenzenesulfonyl) aminobenzoic acid trifluoroacetate
    1-3 2-(6-amidino-2-naphthyloxy)-5-(4-fluorobenzenesulfonyl) aminobenzoic acid trifluoroacetate
    1-4 2-(6-amidino-2-naphthyloxy)-5-(2-naphthalenesulfonyl) aminobenzoic acid trifluoroacetate
    1-5 2-(6-amidino-2-naphthyloxy)-5-(2,3-dichlorobenzene sulfonyl)aminobenzoic acid trifluoroacetate
    1-6 2-(6-amidino-2-naphthyloxy)-5-[4-(phenylsulfonyl) thiophene-2-sulfonyl]aminobenzoic acid trifluoroacetate
    1-7 2-(6-amidino-2-naphthyloxy)-5-(3-nitrobenzenesulfonyl) aminobenzoic acid trifluoroacetate
    1-8 2-(4-amidinophenoxy)-5-(4-methoxybenzenesulfonyl) aminobenzoic acid trifluoroacetate
    1-9 2-(4-amidinophenoxy)-5-(4-fluorobenzenesulfonyl) aminobenzoic acid trifluoroacetate
    1-10 2-(4-amidinophenoxy)-5-(2-naphthalenesulfonyl)aminobenzoic acid trifluoroacetate
    1-11 2-(4-amidinophenoxy)-5-[4-(phenylsulfonyl)thiophene-2-sulfonyl]aminobenzoic acid trifluoroacetate
    1-12 2-(4-amidinophenoxy)-5-methanesulfonylaminobenzoic acid trifluoroacetate
    1-13 2-(3-amidinophenoxy)-5-(4-methoxybenzenesulfonyl) aminobenzoic acid trifluoroacetate
    1-14 2-(3-amidinophenoxy)-5-(4-fluorobenzenesulfonyl) aminobenzoic acid trifluoroacetate
    Example Compound name
    1-15 2-(3-amidinophenoxy)-5-(2-naphthalenesulfonyl) aminobenzoic acid trifluoroacetate
    1-16 2-(3-amidinophenoxy)-5-[4-(phenylsulfonyl)thiophene-2-sulfonyl]aminobenzoic acid trifluoroacetate
    1-17 2-(4'-amidino-4-biphenyloxy)-5-(4-fluorobenzenesulfonyl) aminobenzoic acid trifluoroacetate
    1-18 2-(4'-amidino-4-biphenyloxy)-5-(2-naphthalenesulfonyl) aminobenzoic acid trifluoroacetate
    1-19 2-(4'-amidino-4-biphenyloxy)-5-[4-(phenylsulfonyl) thiophene-2-sulfonyl]aminobenzoic acid trifluoroacetate
    1-20 2-(4'-amidino-4-biphenyloxy)-5-methanesulfonylaminobenzoic acid trifluoroacetate
    1-21 2-[2-(4-amidinophenyl)ethoxy]-5-(4-methoxybenzenesulfonyl) aminobenzoic acid trifluoroacetate
    1-22 2-[2-(4-amidinophenyl)ethoxy]-5-(4-fluorobenzenesulfonyl) aminobenzoic acid trifluoroacetate
    1-23 2-[2-(4-amidinophenyl)ethoxy]-5-(2-naphthalenesulfonyl) aminobenzoic acid trifluoroacetate
    1-24 2-[2-(4-amidinophenyl)ethoxy]-5-[4-(phenylsulfonyl) thiophene-2-sulfonyl]aminobenzoic acid trifluoroacetate
    1-25 2-[2-(4-amidinophenyl)ethoxy]-5-methanesulfonyl aminobenzoic acid trifluoroacetate
    1-26 2'-(6-amidino-2-naphthyloxy)-5'-(4-methoxybenzenesulfonyl) amino-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
    1-27 2'-(6-amidino-2-naphthyloxy)-5'-(4-fluorobenzenesulfonyl) amino-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
    Example Compound name
    1-28 2'-(6-amidino-2-naphthyloxy)-5'-(4-t-butylbenzenesulfonyl) amino-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
    1-29 2'-(6-amidino-2-naphthyloxy)-5'-(4-acetamidobenzene sulfonyl)amino-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
    1-30 2'-(6-amidino-2-naphthyloxy)-5'-(5-chlorothiophene-2-sulfonyl)amino-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
    1-31 2'-(6-amidino-2-naphthyloxy)-5'-(5-chloro-3-methylbenzo[b]thiophene-2-sulfonyl)amino-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
    1-32 2'-(6-amidino-2-naphthyloxy)-5'-(1-methylimidazole-4-sulfonyl)amino-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
    1-33 2'-(6-amidino-2-naphthyloxy)-5'-(4-methanesulfonylbenzene sulfonyl)amino-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
    1-34 2'-(6-amidino-2-naphthyloxy)-5'-(3,4-dimethoxybenzene sulfonyl)amino-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
    1-35 2'-(6-amidino-2-naphthyloxy)-5'-(3-carboxylbenzene sulfonyl)amino-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
    1-36 2'-(6-amidino-2-naphthyloxy)-5'-(4-trifluoromethoxybenzene sulfonyl)amino-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
    Example Compound name
    1-37 2'-(6-amidino-2-naphthyloxy)-5'-(4-trifluoromethylbenzene sulfonyl)amino-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
    1-38 2'-(6-amidino-2-naphthyloxy)-5'-(3-chlorobenzenesulfonyl) amino-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
    1-39 2'-(6-amidino-2-naphthyloxy)-5'-[1,2,3,4-tetrafluoro-2-(acetyl)isoquinoline-7-sulfonyl)amino-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
    1-40 2'-(6-amidino-2-naphthyloxy)-5'-(3-chloro-4-methylbenzenesulfonyl)amino-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
    1-41 2'-(6-amidino-2-naphthyloxy)-5'-(3-fluoro-4-methoxybenzenesulfonyl) amino-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
    1-42 2'-(6-amidino-2-naphthyloxy)-5'-methanesulfonylamino-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
    1-43 2'-(4-amidinophenoxy)-5'-(4-methoxybenzenesulfonyl)amino-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
    1-44 2'-(4-amidinophenoxy)-5'-benzenesulfonylamino-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
    1-45 2'-(4-amidinophenoxy)-5'-methanesulfonylamino-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
    1-46 2'-(6-amidino-2-naphthyloxy)-5'-(4-methoxybenzenesulfonyl) amino-1,1'-biphenyl-3-carboxylic acid trifluoroacetate
    1-47 2'-(6-amidino-2-naphthyloxy)-5'-(4-fluorobenzenesulfonyl) amino-1,1'-biphenyl-3-carboxylic acid trifluoroacetate
    Example Compound name
    1-48 2'-(6-amidino-2-naphthyloxy)-5'-(2-naphthalenesulfonyl) amino-1,1'-biphenyl-3-carboxylic acid trifluoroacetate
    1-49 2'-(6-amidino-2-naphthyloxy)-5'-methanesulfonylamino-1,1'-biphenyl-3-carboxylic acid trifluoroacetate
    1-50 2'-(4-amidinophenoxy)-5'-(4-methoxybenzenesulfonyl)amino-1,1'-biphenyl-3-carboxylic acid trifluoroacetate
    1-51 2'-(4-amidinophenoxy)-5'-benzenesulfonylamino-1,1'-biphenyl-3-carboxylic acid trifluoroacetate
    1-52 2'-(4-amidinophenoxy)-5'-(4-biphenylsulfonyl)amino-1,1'-biphenyl-3-carboxylic acid trifluoroacetate
    1-53 2'-(4-amidinophenoxy)-5'-methanesulfonylamino-1,1'-biphenyl-3-carboxylic acid trifluoroacetate
    1-54 3-[2-(6-amidino-2-naphthyloxy)-5-(4-methoxybenzene sulfonyl)amino-phenyl]-naphthalene-2-carboxylic acid trifluoroacetate
    1-55 3-[2-(6-amidino-2-naphthyloxy)-5-(4-fluorobenzenesulfonyl) amino-phenyl]-naphthalene-2-carboxylic acid trifluoroacetate
    1-56 3-[2-(6-amidino-2-naphthyloxy)-5-(2-naphthalenesulfonyl) amino-phenyl]-naphthalene-2-carboxylic acid trifluoroacetate
    1-57 3-[2-(6-amidino-2-naphthyloxy)-5-methanesulfonylaminophenyl]-naphthalene-2-carboxylic acid trifluoroacetate
    1-58 3-[2-(6-cyano-2-naphthyloxy)-5-(4-methoxybenzenesulfonyl) amino-phenyl]-thiophene-2-carboxylic acid trifluoroacetate
    Example Compound name
    1-59 3-[2-(6-cyano-2-naphthyloxy)-5-(4-fluorobenzenesulfonyl) amino-phenyl]-thiophene-2-carboxylic acid trifluoroacetate
    1-60 3-[2-(6-cyano-2-naphthyloxy)-5-(2-naphthalenesulfonyl) amino-phenyl]-thiophene-2-carboxylic acid trifluoroacetate
    1-61 3-[2-(6-cyano-2-naphthyloxy)-5-methanesulfonylaminophenyl]-thiophene-2-carboxylic acid trifluoroacetate
    1-62 2'-(6-cyano-2-naphthyloxy)-5'-(3-fluoro-4-methoxybenzene sulfonyl)amino-4,5-dimethoxy-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
    1-63 2'-(6-cyano-2-naphthyloxy)-5'-(3-chloro-4-methylbenzene sulfonyl)amino-4,5-dimethoxy-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
    1-64 2'-(6-cyano-2-naphthyloxy)-5'-(2-toluylsulfonyl)amino-4,5-dimethoxy-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
    1-65 2'-(6-cyano-2-naphthyloxy)-5'-methanesulfonylamino-4,5-dimethoxy-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
    1-66 2'-(6-cyano-2-naphthyloxy)-5'-(3-fluoro-4-methoxybenzene sulfonyl)amino-4-(2-methylpropoxy)-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
    1-67 2'-(6-cyano-2-naphthyloxy)-5'-(3-chloro-4-methylbenzene sulfonyl)amino-4-(2-methylpropoxy)-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
    1-68 2'-(6-cyano-2-naphthyloxy)-5'-(2-toluylsulfonyl)amino-4-(2-methylpropoxy)-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
    1-69 2'-(6-cyano-2-naphthyloxy)-5'-methanesulfonylamino-4-(2-methylpropoxy)-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
    • Example 1-34 2'-(6-amidino-2-naphthyloxy)-5'-(3,4-dimethoxybenzenesulfonyl)amino-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
  • Figure 00830001
    1H-NMR (d6 -DMSO) δ : 3.73 (3H, s), 3.81 (3H, s), 6.98 (1H, d, J=9.2Hz), 7.06-7.12 (5H, m), 7.15 (1H, dd, J=2.8, 9.2Hz), 7.22 (1H, d, J=2.4Hz), 7.29-7.34 (2H, m), 7.41 (1H, t, J=7.2Hz), 7.68-7.71 (2H, m), 7.83 (1H, d, J=8.8Hz), 7.92 (1H, d, J=9.2H z), 8.34 (1H, s), 9.04 (2H, brs), 9.31 (2H, brs), 10.10 (1H, s) .
    • Example 1-40 2'-(6-amidino-2-naphthyloxy)-5'-(3-chloro-4-methylbenzenesulfonyl)amino-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
  • Figure 00840001
    1H-NMR (d6-DMSO) δ : 2.38 (3 H, s), 6.99-7.17 (6H, m), 7.32 (1H, t, J=7.6Hz), 7.43 (1H, t, J=7.6Hz), 7.57-7.59 (2H, m), 7.69-7.72 (3H, m), 7.84 (1H, d, J=8.8Hz), 7.93 (1H, d, J=9.2Hz), 8.34 (1H, s), 9.04 (2H, brs), 9.31 (2H, brs), 10.32 (1H, s).
    • Examples 2-1 to 2-4
  • The compound (t-butyl 4-amino-2-(6-cyano-2-naphthyloxy) benzoate) obtained in a previous production example and sulfonyl chloride derivatives represented by the following formula (Ib) : R1-SO2-Cl (where R1 represents the respective R1 groups in Table 19)
    were used for the same reaction as in Example 1-1 to obtain compounds for Examples 2-1 to 2-4, respectively.
    Figure 00850001
    Example Compound name
    2-1 2-(6-amidino-2-naphthyloxy)-4-(4-methoxybenzenesulfonyl) aminobenzoic acid trifluoroacetate
    2-2 2-(6-amidino-2-naphthyloxy)-4-(4-fluorobenzenesulfonyl) aminobenzoic acid trifluoroacetate
    2-3 2-(6-amidino-2-naphthyloxy)-4-(2-naphthalenesulfonyl) aminobenzoic acid trifluoroacetate
    2-4 2-(6-amidino-2-naphthyloxy)-4-(3,4-dichlorobenzene sulfonyl)aminobenzoic acid trifluoroacetate
    • Examples 3-1 to 3-3
  • The compound (t-butyl 4-amino-2-(6-cyano-2-naphthyloxy) benzoate) obtained in a previous production example and acid chloride derivatives represented by the following formula (Ic): R1-CO-Cl (where R1 represents the respective R1 groups in Table 21)
    were used for the same reaction as in Example 1-1 to obtain compounds for Examples 3-1 to 3-3, respectively.
    Figure 00870001
    Example Compound name
    3-1 2-(6-amidino-2-naphthyloxy)-5-(2-thienylcarbonyl) aminobenzoic acid trifluoroacetate
    3-2 2'-(4-amidinophenoxy)-5'-benzoylamino-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
    3-3 2'-(4-amidinophenoxy)-5'-benzoylamino-1,1'-biphenyl-3-carboxylic acid trifluoroacetate
    • Examples 4-1 and 4-2
  • The compounds (t-butyl 5'-amino-2'-(4-cyanophenoxy)-1,1'-biphenyl-2-carboxylate and t-butyl 5'-amino-2'-(4-cyanophenoxy)-1,1'-biphenyl-3-carboxylate) obtained in previous production examples and acid anhydride derivatives represented by the following formula (Id): (R1-CO)2O (where R1 represents the respective R1 groups in Table 23)
    were used for the same reaction as in Example 1-1 to obtain compounds for Examples 4-1 and 4-2, respectively.
    Figure 00880001
    Example Compound name
    4-1 2'-(4-amidinophenoxy)-5'-(3-methylbutanoyl)amino-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
    4-2 2'-(4-amidinophenoxy)-5'-(3-methylbutanoyl)amino-1,1'-biphenyl-3-carboxylic acid trifluoroacetate
    • Example 5-1 2-(6-amidino-2-naphthyloxy)-5-[(3,4-methylenedioxybenzenecarbonyl)amino]benzoic acid trifluoroacetate
  • Figure 00880002
  • After adding 3,4-methylenedioxybenzenecarboxylic acid (9 mg), tetramethylfluoroformamidium hexafluorophosphate (14 mg) and DIEA (10 µl) to an N,N-dimethylformamide solution containing the compound (t-butyl 5-amino-2-(6-cyano-2-naphthyloxy)benzoate) (20 mg) obtained in a previous production example, reaction was conducted at 50°C for 12 hours. The reaction solution was concentrated, a saturated sodium bicarbonate solution was added to the residue, and extraction was performed with ethyl acetate. After freezing the aqueous layer with ethanol-dry ice, the ethyl acetate layer was decanted and the solvent was distilled off. Hydroxyamine hydrochloride (14 mg) and potassium carbonate (15 mg) were added to the obtained residue, and reaction was conducted for 12 hours at 60°C in ethanol:water (2:1, 1 ml). After cooling to room temperature, the reaction solution was concentrated, saturated saline was added to the residue, and extraction was performed with ethyl acetate. After freezing the aqueous layer with ethanol-dry ice, the ethyl acetate layer was decanted and the solvent was distilled off. The obtained residue was dissolved in acetic acid (1 ml), acetic anhydride (8 mg) was added, and reaction was conducted at room temperature for 15 minutes. Next, 10% palladium-carbon (1 mg) was added thereto and catalytic reduction was carried out for 6 hours under ordinary pressure. The catalyst was then filtered off using celite, and the filtrate was concentrated. The residue was dissolved in trifluoroacetic acid:dichloromethane (1:1, 1 ml) and reaction was conducted at room temperature for 90 minutes. The solvent was distilled off and the residue was purified by LC-MS (from 1% acetonitrile/water (0.1% trifluoroacetic acid) to 80% acetonitrile/water (0.1% trifluoroacetic acid), flow rate: 20 ml/min) to obtain 2-(6-amidino-2-naphthyloxy)-5-[(3,4-methylenedioxybenzenecarbonyl)amino]benzoic acid trifluoroacetate.
    RT = 8.44 min, ESI-MS (m/z): 470.1 (M+1)+.
    • Examples 5-2 and 5-3
  • The compound (t-butyl 4-amino-2-(6-cyano-2-naphthyloxy) benzoate) obtained in a previous production example and carboxylic acid derivatives represented by the following formula (Ie): R1-COOH (where R1 represents the respective R1 groups in Table 25)
    were used for the same reaction as in Example 5-1 to obtain compounds for Examples 5-2 and 5-3, respectively.
    Figure 00910001
    Example Compound name
    5-2 2-(6-amidino-2-naphthyloxy)-5-(3-phenylbutanoyl) aminobenzoic acid trifluoroacetate
    5-3 2-(6-amidino-2-naphthyloxy)-5-(6-phenylhexanoyl) aminobenzoic acid trifluoroacetate
    • Examples 6-1 to 6-3
  • The compound (t-butyl 5'-amino-2'-(6-cyano-2-naphthyloxy)-1,1'-biphenyl-2-carboxylate) obtained in a previous production example and isocyanate derivatives represented by the following formula (If): R1-NCO (where R1 represents the respective R1 groups in Table 27)
    were used for the same reaction as in Example 1-1 to obtain compounds for Examples 6-1 to 6-3, respectively.
    Figure 00920001
    Example Compound name
    6-1 2'-(6-amidino-2-naphthyloxy)-5'-(3-chlorophenylureido)-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
    6-2 2'-(6-amidino-2-naphthyloxy)-5'-(3-methoxyphenylureido)-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
    6-3 2'-(6-amidino-2-naphthyloxy)-5'-(cyclohexylureido)-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
    • Example 7 t-Butyl 2-(6-amidino-2-naphthyloxy)-5-[(3,4-dichlorobenzenesulfonyl)amino]benzoate trifluoroacetate
  • Figure 00920002
  • The compound (t-butyl 5-amino-2-(6-cyano-2-naphthyloxy)benzoate) obtained in a previous production example and 3,4-dichlorobenzenesulfonyl chloride instead of methanesulfonyl chloride, were used for treatment by the same method as in Example 2, except for omission of the step of hydrolysis of the ester with TFA, to obtain t-butyl 2-(6-amidino-2-naphthyloxy)-5-[(3,4-dichlorobenzenesulfonyl)amino]benzoate trifluoroacetate.
    RT = 10.42 min, ESI-MS (m/z) : 586.1 (M+1)+.
    • Example 8-1 2'-(6-amidino-2-naphthyloxy)-4-(2-methylpropyl carbamoyl)-5'-methanesulfonylamino-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
  • Figure 00930001
  • Methanesulfonyl chloride (10 µL) was added to a pyridine solution (1 mL) containing the compound (methyl 5'-amino-2'-(6-cyano-2-naphthyloxy)-4-[(2-methylpropyl)carbamoyl]-1,1'-biphenyl-2-carboxylic acid) (20 mg) obtained in a previous production example, and the mixture was stirred at room temperature overnight. Nitrogen gas was sprayed into the reaction solution which was then concentrated. Ethyl acetate and saturated sodium bicarbonate water were added to the residue, and the organic layer was separated off. Next, nitrogen gas was sprayed into the organic layer which was then concentrated. A 1 N lithium hydroxide aqueous solution (0.4 mL), methanol (0.2 mL) and tetrahydrofuran (0.2 mL) were added to the residue, and the mixture was stirred at room temperature overnight. 1 N hydrochloric acid (0.4 mL) was then added to the reaction solution, extraction was performed with ethyl acetate, nitrogen gas was sprayed in and the solution was concentrated. Hydroxyamine hydrochloride (14 mg) and potassium carbonate (15 mg) were added to the obtained residue, and reaction was conducted for 12 hours at 60°C in ethanol:water (2:1, 1 ml). After cooling to room temperature, the reaction solution was concentrated, saturated saline was added to the residue, and extraction was performed with ethyl acetate. After freezing the aqueous layer with ethanol-dry ice, the ethyl acetate layer was decanted and the solvent was distilled off. The obtained residue was dissolved in acetic acid (1 ml), acetic anhydride (8 mg) was added, and reaction was conducted at room temperature for 15 minutes. Next, 10% palladium-carbon (1 mg) was added thereto and catalytic reduction was carried out for 6 hours under ordinary pressure. The catalyst was then filtered off using celite, and the filtrate was concentrated. The residue was dissolved in trifluoroacetic acid:dichloromethane (1:1, 1 ml) and reaction was conducted at room temperature for 90 minutes. The solvent was distilled off and the residue was purified by LC-MS (from 1% acetonitrile/water (0.1% trifluoroacetic acid) to 80% acetonitrile/water (0.1% trifluoroacetic acid), flow rate: 20 ml/min) to obtain 2'-(6-amidino-2-naphthyloxy)-4-(2-methylpropylcarbamoyl)-5'-methanesulfonylamino-1,1'-biphenyl-2-carboxylic acid trifluoroacetate.
    RT = 8.40 min, ESI-MS (m/z) : 575.07 (M+1)+.
    • Examples 8-2 to 8-4
  • The compound (methyl 5'-amino-2'-(6-cyano-2-naphthyloxy)-4-[(2-methylpropyl)carbamoyl]-1,1'-biphenyl-2-carboxylate) obtained in a previous production example and sulfonylchloride derivatives represented by the following formula (Ib): R1-SO2-Cl (where R1 represents the respective R1 groups in Table 29)
    were used for the same reaction as in Example 8-1 to obtain compounds for Examples 8-2 to 8-4, respectively.
    Figure 00960001
    Example Compound name
    8-2 2'-(6-amidino-2-naphthyloxy)-4-(2-methylpropylcarbamoyl)-5'-benzenesulfonylamino-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
    8-3 2'-(6-amidino-2-naphthyloxy)-4-(2-methylpropylcarbamoyl)-5'-(3-chloro-4-methylbenzenesulfonyl)amino-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
    8-4 2'-(6-amidino-2-naphthyloxy)-4-(2-methylpropylcarbamoyl)-5'-(3,4-dichlorobenzenesulfonyl)amino-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
    • Example 9-1 2'-(6-amidino-2-naphthyloxy)-4-(2-methylpropyl carbamoyl)-4'-methyl-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
  • Figure 00960002
  • A 1 N lithium hydroxide aqueous solution (0.4 mL), methanol (0.2 mL) and tetrahydrofuran (0.2 mL) were added to the compound (methyl 2'-(6-cyano-2-naphthyloxy)-4-(2-methylpropylcarbamoyl)-4'-methyl-1,1'-biphenyl-2-carboxylate) (20 mg) obtained above, and the mixture was stirred at room temperature overnight. 1 N hydrochloric acid (0.4 mL) was added to the reaction solution, extraction was performed with ethyl acetate, and nitrogen gas was sprayed into the solution which was then concentrated. Hydroxyamine hydrochloride (14 mg) and potassium carbonate (15 mg) were added to the obtained residue, and reaction was conducted for 12 hours at 60°C in ethanol:water (2:1, 1 ml). After cooling to room temperature, the reaction solution was concentrated, saturated saline was added to the residue, and extraction was performed with ethyl acetate. After freezing the aqueous layer with ethanol-dry ice, the ethyl acetate layer was decanted and the solvent was distilled off. The obtained residue was dissolved in acetic acid (1 ml), acetic anhydride (8 mg) was added, and reaction was conducted at room temperature for 15 minutes. Next, 10% palladium-carbon (1 mg) was added thereto and catalytic reduction was carried out for 6 hours under ordinary pressure. The catalyst was then filtered off using celite, and the filtrate was concentrated. The residue was dissolved in trifluoroacetic acid:dichloromethane (1:1, 1 ml) and reaction was conducted at room temperature for 90 minutes. The solvent was distilled off and the residue was purified by LC-MS (from 1% acetonitrile/water (0.1% trifluoroacetic acid) to 80% acetonitrile/water (0.1% trifluoroacetic acid), flow rate: 20 ml/min) to obtain the title compound.
    RT = 9.20 min, ESI-MS (m/z) : 496.3 (M+1)+.
    • Example 9-2 2'-(4-amidino-2-phenoxy)-4-(2-methylpropyl carbamoyl)-4'-methyl-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
  • Figure 00980001
  • The compound (methyl 2'-(4-cyanophenoxy)-4-(2-methylpropylcarbamoyl)-4'-methyl-1,1'-biphenyl-2-carboxylate) obtained above was used for the same reaction as in Example 9-1 to obtain the title compound. RT = 8.50 min, ESI-MS (m/z): 446.3 (M+1)+.
    Example Compound name
    9-1 2'-(6-amidino-2-naphthyloxy)-4-(2-methylpropylcarbamoyl)-4'-methyl-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
    9-2 2'-(4-amidino-2-phenoxy)-4-(2-methylpropylcarbamoyl)-4'-methyl-1,1'-biphenyl-2-carboxylic acid trifluoroacetate
    • Pharmacological activity test (Inhibiting activity against blood clotting factor VIIa)
  • Compounds of the present invention (Examples 1-4, 1-26, 1-39, 1-40 and 8-3) were each dissolved in dimethyl sulfoxide to 5 mg/ml, and then each solution was diluted 50-fold with reaction buffer to prepare a 100 µg/ml solution (2% dimethyl sulfoxide solution). Also, a 10-fold dilution series with reaction buffer was prepared with the compound solutions from 100 µg/ml to 1 ng/ml. Separately, dimethyl sulfoxide alone was diluted 50-fold with reaction buffer to prepare a 2% dimethyl sulfoxide solution as a control. The composition of the reaction buffer was 50 mM Tris-acetate (pH 7.5), 15 mM calcium chloride (CaCl2), 0.15 M sodium chloride (NaCl) and 6 mg/ml Cephalin. There were also added to the buffer 10 nM human tissue factor (TF) and 5 nM human blood clotting factor VIIa, to prepare enzyme solutions. After separating off 65 µl of each solution, a 10 µl portion of the 2% dimethyl sulfoxide solution of each compound of the invention was added, and preincubation was performed at room temperature. As a substrate there was added 25 µl of 1.0 mM Spectrozyme VII, and reaction was conducted for 40 minutes at room temperature. The enzyme reaction rate was quantified by the change in absorbance of released 4-nitroanilide at 405 nm.
  • For measurement of the inhibiting activity against human blood clotting factor VIIa, the enzyme reaction rate was determined in the presence of the above-mentioned compounds of the present invention from 10 µg/ml to 0.1 ng/ml. The IC50 values were calculated based on nonlinear regression analysis, as an index of the inhibiting activity against human blood clotting factor VIIa. The results are shown in Table 32.
    Compound IC50 (µM)
    1-4 1.43
    1-26 0.21
    1-39 0.03
    1-40 0.04
    8-3 0.004
  • As is clear from the results shown in Table 32, the novel amidino derivatives of the present invention were confirmed to exhibit excellent inhibiting activity against blood clotting factor VIIa.
  • According to the present invention it is possible to obtain novel amidino derivatives having serine protease inhibiting activity and especially excellent inhibiting activity against blood clotting factor VIIa, as well as their pharmacologically acceptable salts or solvates.
  • These amidino derivatives of the present invention and their pharmacologically acceptable salts or solvates are useful as prophylactic and/or therapeutic agents for clinical conditions involving thrombogenesis in which extrinsic clotting mechanisms are implicated, and therefore the present invention provides blood clotting factor VIIa inhibitors, anticoagulants (particularly anticoagulants with inhibiting activity against blood clotting factor VIIa) and thrombosis therapeutic agents which are effective for prevention and/or treatment of clinical conditions involving thrombogenesis.

Claims (18)

  1. An amidino derivative represented by the following general formula (I):
    Figure 01020001
    {where X represents C1-6 alkyl, a halogen atom, -NH2 or a group represented by the following formula:
    Figure 01020002
    (where R1 represents optionally substituted C1-6 alkyl, optionally substituted C1-6 alkoxy, optionally substituted C3-8 cycloalkyl, optionally substituted C6-14 aryl, optionally substituted C7-20 aralkyl, an optionally substituted 5- to 14-membered aromatic heterocyclic group or a group represented by the following formula:
    Figure 01020003
    (where R4 represents hydrogen atom, C1-6 alkyl or optionally substituted C2-7 acyl),
    R2 represents hydrogen atom or C1-6 alkyl, and
    X2 represents -CO-, -SO2-, -NH-CO- or a single bond),
    R3 represents hydrogen atom, hydroxyl, C2-7 acyl or C2-7 alkoxycarbonyl,
    Y represents a group of the formula -Ar2-CO2R5 (where Ar2 represents optionally substituted C6-14 aryl, an optionally substituted 5- to 14-membered aromatic heterocyclic group or a single bond and R5 represents hydrogen atom or C1-6 alkyl), and
    Ar1 represents 2,6-naphthylene, 1,4-phenylene, 1,3-phenylene, a group represented by the following formula:
    Figure 01030001
    or a group represented by the following formula:
    Figure 01030002
    or a pharmacologically acceptable salt or solvate thereof.
  2. An amidino derivative or a pharmacologically acceptable salt or solvate thereof according to claim 1, wherein said amidino derivative is represented by the following general formula (II):
    Figure 01040001
    (where X, Y, Ar1 and R3 have the same definitions as X, Y, Ar1 and R3 in claim 1).
  3. An amidino derivative or a pharmacologically acceptable salt or solvate thereof according to claim 1, wherein said amidino derivative is represented by the following general formula (III):
    Figure 01040002
    (where X, Y and R3 have the same definitions as X, Y and R3 in claim 1).
  4. An amidino derivative or a pharmacologically acceptable salt or solvate thereof according to claim 1, wherein R3 is hydrogen atom.
  5. An amidino derivative or a pharmacologically acceptable salt or solvate thereof according to claim 1, wherein X is C1-6 alkyl or a group represented by the formula R1-SO2NH- (where R1 has the same definition as R1 in claim 1).
  6. An amidino derivative or a pharmacologically acceptable salt or solvate thereof according to claim 1, wherein X is a group represented by the formula R1-SO2NH- {where R1 represents optionally substituted C6-14 aryl or a group represented by the following formula:
    Figure 01050001
    (where R4 represents optionally substituted C2-7 acyl)}.
  7. An amidino derivative or a pharmacologically acceptable salt or solvate thereof according to claim 1, wherein Y is carboxyl or optionally substituted carboxyphenyl.
  8. An amidino derivative or a pharmacologically acceptable salt or solvate thereof according to claim 1, wherein Y is a group represented by the following formula:
    Figure 01060001
  9. A blood clotting factor VIIa inhibitor comprising an amidino derivative or a pharmacologically acceptable salt or solvate thereof according to claim 1.
  10. An anticoagulant containing, as an active ingredient, an amidino derivative or a pharmacologically acceptable salt or solvate thereof according to claim 1.
  11. A thrombosis therapeutic agent containing, as an active ingredient, an amidino derivative or a pharmacologically acceptable salt or solvate thereof according to claim 1.
  12. A compound library comprising an amidino derivative or a pharmacologically acceptable salt or solvate thereof according to claim 1.
  13. A compound library comprising 1 to 1 million amidino derivatives or pharmacologically acceptable salts or solvates thereof according to claim 1.
  14. The use of an amidino derivative or a pharmacologically acceptable salt or solvate thereof according to claim 1 as a blood clotting factor VIIa inhibitor.
  15. The use of an amidino derivative or a pharmacologically acceptable salt or solvate thereof according to claim 1 as an active ingredient of an anticoagulant.
  16. The use of an amidino derivative or a pharmacologically acceptable salt or solvate thereof according to claim 1 as an active ingredient of a thrombosis therapeutic agent.
  17. A treatment method for clinical conditions involving thrombogenesis in which extrinsic blood clotting mechanisms are implicated, which involves administering an amidino derivative or a pharmacologically acceptable salt or solvate thereof according to claim 1.
  18. A prophylactic method for clinical conditions involving thrombogenesis in which extrinsic blood clotting mechanisms are implicated, which involves administering an amidino derivative or a pharmacologically acceptable salt or solvate thereof according to claim 1.
EP02025580A 2001-11-15 2002-11-15 Amidino derivatives, and anticoagulants and thrombosis therapeutic agents containing them Withdrawn EP1312602A1 (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7807690B2 (en) 2007-09-21 2010-10-05 Eisai R&D Management Co., Ltd. 2,3-dihydro-iminoisoindole derivatives
US7816522B2 (en) 2006-03-24 2010-10-19 Eisai Co., Ltd. Triazolone derivatives

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998001422A1 (en) * 1996-07-04 1998-01-15 Astra Aktiebolag New amidino derivatives and their use as thrombin inhibitors
WO1999041231A1 (en) * 1998-02-17 1999-08-19 Ono Pharmaceutical Co., Ltd. Amidino derivatives and drugs containing the same as the active ingredient
WO2001019788A2 (en) * 1999-09-17 2001-03-22 Cor Therapeutics, Inc. BENZAMIDES AND RELATED INHIBITORS OF FACTOR Xa

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998001422A1 (en) * 1996-07-04 1998-01-15 Astra Aktiebolag New amidino derivatives and their use as thrombin inhibitors
WO1999041231A1 (en) * 1998-02-17 1999-08-19 Ono Pharmaceutical Co., Ltd. Amidino derivatives and drugs containing the same as the active ingredient
EP1078917A1 (en) * 1998-02-17 2001-02-28 Ono Pharmaceutical Co., Ltd. Amidino derivatives and drugs containing the same as the active ingredient
WO2001019788A2 (en) * 1999-09-17 2001-03-22 Cor Therapeutics, Inc. BENZAMIDES AND RELATED INHIBITORS OF FACTOR Xa

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7816522B2 (en) 2006-03-24 2010-10-19 Eisai Co., Ltd. Triazolone derivatives
US7928228B2 (en) 2006-03-24 2011-04-19 Eisai Co., Ltd. Triazolone derivatives
US8163787B2 (en) 2006-03-24 2012-04-24 Eisai R&D Management Co., Ltd. Triazolone derivatives
US7807690B2 (en) 2007-09-21 2010-10-05 Eisai R&D Management Co., Ltd. 2,3-dihydro-iminoisoindole derivatives

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