EP1279961A3 - Non-isotopic method and kit for quantifying enzyme inhibitors - Google Patents

Non-isotopic method and kit for quantifying enzyme inhibitors Download PDF

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Publication number
EP1279961A3
EP1279961A3 EP02255265A EP02255265A EP1279961A3 EP 1279961 A3 EP1279961 A3 EP 1279961A3 EP 02255265 A EP02255265 A EP 02255265A EP 02255265 A EP02255265 A EP 02255265A EP 1279961 A3 EP1279961 A3 EP 1279961A3
Authority
EP
European Patent Office
Prior art keywords
therapeutic compound
kit
biological substance
reaction vessel
probe
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP02255265A
Other languages
German (de)
French (fr)
Other versions
EP1279961A2 (en
Inventor
Zhibo Gan
Jeanette Wallman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Norzyme Inc
Original Assignee
Norzyme Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Norzyme Inc filed Critical Norzyme Inc
Publication of EP1279961A2 publication Critical patent/EP1279961A2/en
Publication of EP1279961A3 publication Critical patent/EP1279961A3/en
Withdrawn legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/81—Protease inhibitors
    • G01N2333/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • G01N2333/8142—Aspartate protease (E.C. 3.4.23) inhibitors, e.g. HIV protease inhibitors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
    • G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
    • G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
    • G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
    • G01N2333/96472—Aspartic endopeptidases (3.4.23)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A method, kit, and assay for measuring levels of a therapeutic compound in a sample by providing a reaction vessel containing a sample, the sample includes a therapeutic compound having a biological activity and biological substance. A probe coated with a substrate for the biological substance is provided, the substrate having a detectable label. The probe is inserted into the reaction vessel such that the therapeutic compound and the substrate contact the biological substance and interact within the biological substance resulting in the label being released. The probe is removed from the reaction vessel and the quantity of detectable label is measured, thereby measuring the level of therapeutic compound. The concentration of therapeutic compound is calculated based upon a standard curve or by calculating the percentage of inhibition and expressing the results in arbitrary units.
EP02255265A 2001-07-23 2002-07-23 Non-isotopic method and kit for quantifying enzyme inhibitors Withdrawn EP1279961A3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US30757001P 2001-07-23 2001-07-23
US307570 2001-07-23

Publications (2)

Publication Number Publication Date
EP1279961A2 EP1279961A2 (en) 2003-01-29
EP1279961A3 true EP1279961A3 (en) 2004-02-18

Family

ID=23190315

Family Applications (1)

Application Number Title Priority Date Filing Date
EP02255265A Withdrawn EP1279961A3 (en) 2001-07-23 2002-07-23 Non-isotopic method and kit for quantifying enzyme inhibitors

Country Status (2)

Country Link
US (1) US20030027193A1 (en)
EP (1) EP1279961A3 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100860958B1 (en) * 2007-08-08 2008-09-30 전남대학교산학협력단 Multi-channel Small Bioreactor with Optical Sensor Membrane Attachment
US11673134B2 (en) * 2017-06-06 2023-06-13 Northwestern University Trans-interfacial magnetic separation
CN110286057A (en) * 2019-06-06 2019-09-27 武汉纺织大学 A portable carbonate intelligent testing device and method

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5376531A (en) * 1992-09-03 1994-12-27 Northwestern University Method of detecting cancer
WO1999054734A1 (en) * 1998-04-16 1999-10-28 Norzyme Inc. Comb-like solid phase for measuring analytes

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL77144A (en) * 1984-11-27 1991-04-15 Orgenics Ltd Method and apparatus for performing solid phase immuno-assay on test card in a plurality of samples
US5559252A (en) * 1994-02-16 1996-09-24 The Dupont Merck Pharmaceutical Company Process for the preparation of diamine intermediates useful in the synthesis of HIV protease inhibitors

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5376531A (en) * 1992-09-03 1994-12-27 Northwestern University Method of detecting cancer
WO1999054734A1 (en) * 1998-04-16 1999-10-28 Norzyme Inc. Comb-like solid phase for measuring analytes

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
FOULDS G ET AL: "THE EFFECT OF AZITHROMYCIN ON THE PHARMACOKINETICS OF INDINAVIR", JOURNAL OF CLINICAL PHARMACOLOGY, LIPPINCOTT CO, HAGERSTOWN, MD, US, vol. 39, no. 8, 1999, pages 842 - 846, XP001010173, ISSN: 0091-2700 *
GAN ET AL: "Rapid fluorometric method for the measurement of HIV protease inhibitor levels in patient serum", XIV INTERNATIONAL AIDS CONFERENCE 2002, BARCELONA, 7 July 2002 (2002-07-07) - 12 July 2002 (2002-07-12), pages WePeB5871, XP002263532, Retrieved from the Internet <URL:http://www.aids2002.com/Program/ViewAbstract.asp?id=/T-CMS_Content/Abstract/200206290751316085.xml> [retrieved on 20031202] *
NOVAK ET AL: "Functional measurement of HIV protease inhibitor levels in patient serum by a rapid fluorometric method", XIV INTERNATIONAL AIDS CONFERENCE 2002, BARCELONA, 7 July 2002 (2002-07-07) - 12 July 2002 (2002-07-12), pages WePeB5872, XP002263533, Retrieved from the Internet <URL:http://www.aids2002.com/Program/ViewAbstract.asp?id=/T-CMS_Content/Abstract/200206290751164558.xml> [retrieved on 20031202] *
WILTSHIRE H R ET AL: "Chromatographic and immunochemical approaches to the analysis of the HIV protease inhibitor saquinavir in plasma", ANALYTICAL BIOCHEMISTRY, vol. 281, no. 1, 15 May 2000 (2000-05-15), pages 105 - 114, XP002263534, ISSN: 0003-2697 *

Also Published As

Publication number Publication date
EP1279961A2 (en) 2003-01-29
US20030027193A1 (en) 2003-02-06

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