EP1263934A4 - Genetic engineering of drought tolerance via a plastid genome - Google Patents

Genetic engineering of drought tolerance via a plastid genome

Info

Publication number
EP1263934A4
EP1263934A4 EP01913108A EP01913108A EP1263934A4 EP 1263934 A4 EP1263934 A4 EP 1263934A4 EP 01913108 A EP01913108 A EP 01913108A EP 01913108 A EP01913108 A EP 01913108A EP 1263934 A4 EP1263934 A4 EP 1263934A4
Authority
EP
European Patent Office
Prior art keywords
genetic engineering
drought tolerance
plastid genome
tolerance via
plastid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP01913108A
Other languages
German (de)
French (fr)
Other versions
EP1263934A1 (en
Inventor
Henry Daniell
Seung-Bum Lee
Myung Ok Byun
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Auburn University
University of Central Florida
Original Assignee
Auburn University
University of Central Florida
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Auburn University, University of Central Florida filed Critical Auburn University
Publication of EP1263934A1 publication Critical patent/EP1263934A1/en
Publication of EP1263934A4 publication Critical patent/EP1263934A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Nutrition Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP01913108A 2000-02-29 2001-02-28 Genetic engineering of drought tolerance via a plastid genome Withdrawn EP1263934A4 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US18565800P 2000-02-29 2000-02-29
US185658P 2000-02-29
PCT/US2001/006271 WO2001064850A1 (en) 2000-02-29 2001-02-28 Genetic engineering of drought tolerance via a plastid genome

Publications (2)

Publication Number Publication Date
EP1263934A1 EP1263934A1 (en) 2002-12-11
EP1263934A4 true EP1263934A4 (en) 2004-05-06

Family

ID=22681915

Family Applications (1)

Application Number Title Priority Date Filing Date
EP01913108A Withdrawn EP1263934A4 (en) 2000-02-29 2001-02-28 Genetic engineering of drought tolerance via a plastid genome

Country Status (4)

Country Link
EP (1) EP1263934A4 (en)
AU (1) AU2001241809A1 (en)
CA (1) CA2401855A1 (en)
WO (1) WO2001064850A1 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100251425A9 (en) 1998-05-15 2010-09-30 University Of Central Florida Expression of human interferon in transgenic chloroplasts
US20040187175A1 (en) * 2002-11-06 2004-09-23 Wu Ray J. TPS plant gene constructs and transformants
KR100440725B1 (en) 2002-06-20 2004-07-15 주식회사 그린진 바이오텍 A Method for Increasing an Abiotic-Resistance in Monocot Plants
CA2491639A1 (en) 2002-07-03 2004-01-15 University Of Central Florida Expression of the human igf-1 in transgenic plastids
WO2004013333A2 (en) 2002-07-26 2004-02-12 Basf Plant Science Gmbh Inversion of the negative-selective effect of negative marker proteins using selection methods
EP1885173A4 (en) 2005-05-27 2009-02-18 Univ Central Florida Chloroplasts engineered to express pharmaceutical proteins
US10752909B2 (en) 2007-03-30 2020-08-25 The Trustees Of The University Of Pennsylvania Chloroplasts engineered to express pharmaceutical proteins in edible plants
US8314222B2 (en) * 2007-10-05 2012-11-20 Sapphire Energy, Inc. System for capturing and modifying large pieces of genomic DNA and constructing organisms with chloroplasts
JP2011511090A (en) 2008-02-07 2011-04-07 セレジーン インコーポレイテッド Photoreceptor rescue method by intravitreal administration of an expression vector encoding a therapeutic protein
US10689633B2 (en) 2008-02-29 2020-06-23 The Trustees Of The University Of Pennsylvania Expression of β-mannanase in chloroplasts and its utilization in lignocellulosic woody biomass hydrolysis
WO2010033275A2 (en) * 2008-05-27 2010-03-25 University Of Central Florida Research Foundation, Inc. Orally administerable vaccine for yersinia pestis
CN101289514B (en) * 2008-06-13 2011-01-19 北京北方杰士生物科技有限责任公司 Process for cultivating stress-tolerant plants and special DNA fragments thereof
WO2011057243A2 (en) 2009-11-09 2011-05-12 University Of Central Florida Research Foundation, Inc. Administration of plant expressed oral tolerance agents
US10865419B2 (en) 2011-10-24 2020-12-15 The Trustees Of The University Of Pennsylvania Orally administered plastid expressed cholera toxin B subunit-exendin 4 as treatment for type 2 diabetes
CN105492458B (en) * 2013-09-17 2019-04-16 创世纪种业有限公司 A kind of cotton trehalose synthetase TPS-2 and its encoding gene and application

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999010513A1 (en) * 1997-08-07 1999-03-04 Auburn University Universal chloroplast integration and expression vectors, transformed plants and products thereof
WO1999046370A2 (en) * 1998-03-11 1999-09-16 Novartis Ag Expression of trehalose biosynthetic genes in plants

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5693507A (en) * 1988-09-26 1997-12-02 Auburn University Genetic engineering of plant chloroplasts
DE69233380T2 (en) * 1991-05-09 2005-07-28 ARIZONA BOARD OF REGENTS, on behalf of THE UNIVERSITY OF ARIZONA, Tucson TRANSGENIC PLANTS WITH CHANGED POLYOL CONTENT
US5792921A (en) * 1992-02-14 1998-08-11 Londesborough; John Increasing the trehalose content of organisms by transforming them with combinations of the structural genes for trehalose synthase
US5780709A (en) * 1993-08-25 1998-07-14 Dekalb Genetics Corporation Transgenic maize with increased mannitol content

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999010513A1 (en) * 1997-08-07 1999-03-04 Auburn University Universal chloroplast integration and expression vectors, transformed plants and products thereof
WO1999046370A2 (en) * 1998-03-11 1999-09-16 Novartis Ag Expression of trehalose biosynthetic genes in plants

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
LEE SEUNG-BUM ET AL: "Accumulation of trehalose within transgenic chloroplasts confers drought tolerance.", MOLECULAR BREEDING, vol. 11, no. 1, January 2003 (2003-01-01), pages 1 - 13, XP009027206, ISSN: 1380-3743 *
NUCCIO M L ET AL: "Metabolic engineering of plants for osmotic stress resistance", CURRENT OPINION IN PLANT BIOLOGY, QUADRANT SUBSCRIPTION SERVICES, GB, vol. 2, no. 2, April 1999 (1999-04-01), pages 128 - 134, XP002216348, ISSN: 1369-5266 *
RATHINASABAPATHI B ET AL: "METABOLIC ENGINEERING OF GLYCINE BETAINE SYNTHESIS: PLANT BETAINE ALDEHYDE DEHYDROGENASES LACKING TYPICAL TRANSIT PEPTIDES ARE ARGETED TO TOBACCO CHLOROPLASTS WHERE THEY CONFER BETAINE ALDEHYDE RESISTANCE", PLANTA, SPRINGER VERLAG, DE, vol. 193, 1994, pages 155 - 162, XP002034434, ISSN: 0032-0935 *
See also references of WO0164850A1 *

Also Published As

Publication number Publication date
EP1263934A1 (en) 2002-12-11
AU2001241809A1 (en) 2001-09-12
CA2401855A1 (en) 2001-09-07
WO2001064850A1 (en) 2001-09-07

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