EP1231939A2 - Oncolytic combinations for the treatment of cancer - Google Patents

Oncolytic combinations for the treatment of cancer

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Publication number
EP1231939A2
EP1231939A2 EP00983695A EP00983695A EP1231939A2 EP 1231939 A2 EP1231939 A2 EP 1231939A2 EP 00983695 A EP00983695 A EP 00983695A EP 00983695 A EP00983695 A EP 00983695A EP 1231939 A2 EP1231939 A2 EP 1231939A2
Authority
EP
European Patent Office
Prior art keywords
ethyl
propoxy
fluorophenyl
hydroxyphenoxy
phenyl
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP00983695A
Other languages
German (de)
French (fr)
Inventor
Jerome Herbert Fleisch
Jason Scott Sawyer
Beverly Ann Teicher
Douglas Wade Beight
Edward C. R. Smith
William Thomas Mcmillen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eli Lilly and Co
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Eli Lilly and Co
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Filing date
Publication date
Application filed by Eli Lilly and Co filed Critical Eli Lilly and Co
Publication of EP1231939A2 publication Critical patent/EP1231939A2/en
Withdrawn legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
    • A61K45/06—Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/21—Esters, e.g. nitroglycerine, selenocyanates
    • A61K31/215—Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids
    • A61K31/235—Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids having an aromatic ring attached to a carboxyl group
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Definitions

  • This invention relates to a method of treating cancer with anti-cancer agents. More specifically, it relates to the use of anti-cancer agents, in conjunction with leukotriene (LTB4) antagonists which enhance the effectiveness of the anti-cancer agents.
  • LTB4 leukotriene
  • Leukotriene B4 is a proinflammatory lipid which has been implicated in the pathogenesis of psoriasis, arthritis, chronic lung diseases, acute respiratory distress syndrome, shock, asthma, inflammatory bone diseases and other inflammatory states characterized by the infiltration and activation of polymorphonuclear leukocytes and other proinflammatory cells. Thus activated, the polymorphonuclear leukocytes liberate tissue-degrading enzymes and reactive chemicals causing the inflammation.
  • US Patent 5,462,954 discloses phenylphenol leukotriene antagonists which are useful in the treatment of psoriasis, arthritis, chronic lung diseases, acute respiratory distress syndrome, shock, asthma, inflammatory bone diseases and other inflammatory states characterized by the infiltration and activation of polymorphonuclear leukocytes and other proinflammatory cells.
  • US Patent 5,910,505 discloses that certain phenylphenol leukotriene B4 (LTB4) antagonists are useful as agents for the treatment of oral squamous cell carcinoma.
  • US Patent 5,543,428 discloses a group of phenylphenol leukotriene antagonists that have the property of reversing multi-drug resistance in tumor cells.
  • the use of the leukotriene antagonist will reverse the drug resistance of resistant tumor cells to vinblastine, vincristine, vindesine, navelbine, daunorubicin, doxorubicin, mitroxantrone, etoposide, teniposide, mitomycin-C, actinomycin-D, taxol, topotecan, mithramycin, colchicine, puromycin, podophylotoxin, emetine, gramicidin-D, and valinomycin.
  • compositions and methods useful for treating cancers which are not multi-drug resistant.
  • the compositions of the present invention include anti- cancer agents in combination with leukotriene (LTB4) antagonists of formula A, formula I and formula II.
  • LTB4 leukotriene
  • Acidic Group means an organic group which when attached as the "Z" substituent of formula (I) or the "Z2" substituent of formula (II) acts as a proton donor capable of hydrogen bonding.
  • An illustrative acidic group is carboxyl .
  • Active Ingredient refers both to certain anti-cancer agents defined below and also leukotriene B4 antagonist compounds generically described by formula A as well as diphenyl leukotriene B4 antagonist compounds generically described by formula A, formula I and formula II or the list of specific diphenyl compounds disclosed, infra., as well as a combination of a anti-cancer agent and a leukotriene B4 antagonist described by formula A or formula I or II, and the salts, solvates, and prodrugs of such compounds .
  • alkenyl means a monovalent radical of the generic formula C n H2 n such as ethenyl, n-propenyl, isopropeneyl, n-butenyl, isobutenyl, 2-butenyl, and 3-butenyl .
  • alkyl by itself or as part of another substituent means, unless otherwise defined, a straight or branched chain monovalent hydrocarbon radical such as methyl, ethyl, n-propyl , isopropyl, n-butyl, tertiary butyl, sec-butyl, n-pentyl, and n-hexyl .
  • alkaryl means an aryl radical substituted with an alkyl or substituted aryl group, for example:
  • C5-C20 alkaryl the numerical subscripts refer to the total number of carbon atoms in the radical.
  • C5-C20 aralkyl means an alkyl radical substituted with an aryl or substituted aryl group, for example :
  • C5-C20 aralkyl the numerical subscripts refer to the total number of carbon atoms in the radical .
  • Carbocyclic group refers to a five, six, seven, or eight membered saturated, unsaturated or aromatic ring containing only carbon and hydrogen (e.g., benzene, cyclohexene, cyclohexane, cyclopentane) .
  • cycloalkyl means a carbocyclic non- aromatic monovalent radical such as cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, and cyclooctyl .
  • halo means fluoro, chloro, bromo, or iodo
  • heterocyclic radical refers to a radical having a saturated, unsaturated or aromatic five membered substituted or unsubstituted ring containing from 1 to 4 hetero atoms .
  • mammal and “mammalian” include human.
  • N-sulfonamidyl means the radical
  • R12 is C]_-C ⁇ o alkyl, aryl, C1-C6 alkyl substituted aryl, C5-C20 alkaryl, or C5-C20 aralkyl.
  • substituted alkyl means an alkyl group further substituted with one or more radical (s) selected from halo, C1-C alkyl, aryl, benzyl, C2-C5 alkenyl, C2-C6 alkynyl, C3-C8 cycloalkyl, alkoxy, C1-C haloalkyl (e.g. , -CF3) .
  • radical selected from halo, C1-C alkyl, aryl, benzyl, C2-C5 alkenyl, C2-C6 alkynyl, C3-C8 cycloalkyl, alkoxy, C1-C haloalkyl (e.g. , -CF3) .
  • substituted aryl means an aryl group further substituted with one or more radical (s) selected from halo, C1-C alkyl, aryl, benzyl, C2-C alkenyl, C2-C5 alkynyl, C3-C8 cycloalkyl, C]_-Cs alkoxy, C1-C haloalkyl (e.g. , -CF3) .
  • tetrazolyl refers to an acidic group represented by either of the formulae:
  • terapéuticaally effective interval is a period of time beginning when one of either (a) the anti- cancer agent or (b) the LTB4 antagonist is administered to a mammal and ending at the limit of the anti-cancer beneficial effect in treating cancer of (a) or (b) .
  • the anti-cancer agents and the leukotriene (LTB4) antagonist are administered within 24 hours of each other, more preferably within 4 hours and most preferably within 1 hour.
  • terapéuticaally effective combination means administration of both (a) the anti-cancer agent and (b) the LTB4 antagonist, either simultaneously or separately.
  • the types of cancers which may be treated with the compositions of the present invention include: Breast Carcinoma, Bladder Carcinoma, Colorectal Carcinoma, Esophageal Carcinoma, Gastric Carcinoma, Germ Cell Carcinoma e.g.
  • Testicular Cancer Gynecologic Carcinoma, Lymphoma - Hodgkin's, Lymphoma - Non-Hodgkin' s, Malignant Melanoma, Multiple Myeoma, Neurologic Carcinoma, Brain Cancer, Non-Small Cell Lung Cancer, Pancreatic Carcinoma, Prostate Carcinoma, Ewings Sarcoma, Osteosarcoma, Soft Tissue Sarcoma, Pediatric Malignancies and the like.
  • anti cancer agents which may be used include:
  • ALKYLATING AGENTS Busulfan, Carboplatin, Carmustine, Cisplatin, Cyclophosphamide, dacarbazine, Ifosfamide, Lomustine, Streptozocin, Oxaliplatin, Temozolomide;
  • ANTIBIOTICS Bleomycin, Dactinomycin, Daunorubicin, Doxorubicin, Idarubicin, Mitomycin-C, Plicamycin, Cryptophycin;
  • ANTIMETABOLITES Cytarabine, Floxuridine, Fludarabine, 5- Fluorouracil, Hydroxyurea, 6-Mercaptopurine, Methotrexate, Thioguanine; Capecitabine;
  • BIOLOGICALS Aldesleukin, Interferon Alfa-2A, Interleukin- 2, Interleukin-12 (recombinant), Interferon Alfa-2B (recombinant), Interferon Alfa-n3, Interferon Gamma-IB, Herceptin interleukin-2 , interleukin-12;
  • HORMONAL AGENTS Aminoglutethimide, Anastrozole, Flutamide, Goserelin, Leuprolide, Megestrol, Mitotane, Tamoxifen;
  • NITROGEN MUSTARD DERIVATIVES Chlorambucil , Estramustine, Mechlorethamine, Melphalan, Thiotepa; PLANT ALKALOIDS: Docetaxel, Etoposide, Irinotecan HCl, Paclitaxel, Teniposide, Topotecan, Vinblastine, Vincristine, Vinorelbine;
  • OTHERS Altretamine, Amifostine Asparaginase-Escheri ⁇ ia coli strain, BCG Live (Intravesical) , Cladribine, Leucovorin, Levamisole, Mitoxantrone, Pegaspargase, Pentostatin, Procarbazine, and the like.
  • the anti-cancer agents may be used alone or in combinations of one or more anti-cancer agents. When used in combination, the anti-cancer agents may be administered at the same time, sequentially or in more complicated regimens where the agents may be administered alternately. Such combinations and dosing regimens are well known to those skilled in the art.
  • the anti-cancer agents may be formulated with common excipients, diluents or carriers, and compressed into tablets, or formulated elixirs or solutions for convenient oral administration or administered by intramuscular intravenous routes.
  • the compounds can be administered transdermally and may be formulated as sustained relief dosage forms and the like.
  • compositions of the present invention are a combination of therapeutically effective amounts of the leukotriene (LTB4) antagonists, noted above, and a therapeutically effective amount of an anti-cancer agent.
  • the composition may be formulated with common excipients, diluents or carriers, and compressed into tablets, or formulated elixirs or solutions for convenient oral administration or administered by intramuscular intravenous routes.
  • the compounds can be administered transdermally and may be formulated as sustained relief dosage forms and the like.
  • the invention in another embodiment, relates to a method of treating a patient suffering from a non-multi-drug resistant cancerous condition which comprises the separate administration of a therapeutically effective amount of the leukotriene (LTB4) antagonists, and the anti-cancer agent.
  • the leukotriene (LTB4) antagonists, and the anti-cancer agent may be administered on a different schedule. One may be administered before the other as long as the time between the two administrations falls within a therapeutically effective interval.
  • Therapeutically effective interval is a period of time beginning when one of either (a) the leukotriene (LTB4) antagonists or (b) the anti-cancer agent is administered to a human and ending at the limit of the beneficial effect in the treatment of cancer of the combination of (a) and (b) .
  • LTB4 leukotriene
  • the methods of administration of the leukotriene LTB4 antagonist and the anti-cancer agent may vary.
  • one agent may be administered orally, while the other is administered intravenously.
  • one of the products may be administered as a continuous infusion while the other is provided in discreet dosage forms.
  • the anti-cancer drug be given in the manner known to optimize its performance.
  • the leukotriene (LTB4) antagonists useful in the present invention include those given in formula A.
  • R4 ' is Rg or taken from one of the following formulae:
  • each Rg is independently -COOH, 5-tetrazolyl, CON ( R 9 ) 2 . or -CONHSO2R10; each R7 is hydrogen, C1-C4 alkyl, C2-C5 alkenyl, C2-C5 alkynyl, benzyl, methoxy, -W-R ⁇ , -T-G-R6, (C1-C4 alkyl )-T- (C1-C4 alkylidenyl) -0-, or hydroxy;
  • R8 is hydrogen or halo; each R9 is independently hydrogen, phenyl, or C1-C4 alkyl, or when taken together with the nitrogen atom form a morpholino, piperidino, piperazino, or pyrrolidino group;
  • R ⁇ is C1-C4 alkyl or phenyl
  • R4 ' is selected from the following formulae:
  • Preferred compounds or pharmaceutically acceptable acid salt derivatives thereof are those wherein said compound selected from the group (A) to (KKKK) consisting of:
  • AAA 2- [2-Propyl-3- [3- (2-ethyl-5-hydroxy-4- phenylphenoxy) propoxy] phenoxy]benzoic acid sodium salt hemihydrate;
  • HHH 3- [4- [9-0x0-3- [3- [2-ethyl-4- (4- fluorophenyl) -5-hydroxyphenoxy] propoxy] -9H- xanthene] ] propanoic acid; III) 3- [2- [l-[2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy] propoxy] -4- (5-oxo-5- morpholinopentanamido) phenyl] propanoic acid; JJJ) 2-Fluoro-6- [2-propyl-3- [3- [2-ethyl-5- hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] phenoxy] benzoic acid disodium salt hydrate;
  • AAAA 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) -E-propenoic acid;
  • LTB4 leukotriene
  • LTB4 antagonists are well known in the art, and are fully described in U.S. Patent 5,462,954, which is hereby specifically incorporated by reference for its disclosure of the methods of preparation of specific leukotriene B 4 antagonists and compounds or formulations of the leukotriene antagonists which may be administered to patients.
  • a preferred compound is 2- [2-propyl-3- [3- [2- ethy1-5-hydroxy-4- ( 4-f1ouropheny1 ) phenoxy] propoxy] phenoxy] benzoic acid which can also be named 2- [3- [3- (5-ethyl-4 ' - flouro-2-hydroxybiphen-4-yloxy) propoxy] -2- propylphhenoxy] benzoic acid, described in U.S. Patent 5,462,954 as example 66 and also shown below as Compound A (Formula B) :
  • a second class of LTB4 antagonists to use as the essential co-agent in the compositions and practice of the method of this invention are those disclosed in copending provisional patent application, titled, "Heterocycle Substituted Diphenyl Leukotriene Antagonists" (inventor, Jason Scott Sawyer) containing 97 pages and identified as Eli Lilly and Company Docket No. B-13240), filed on November 11, 1999, and now Provisional patent Application Serial Number 60/164,786.
  • the subject Heterocycle substituted diphenyl leukotriene antagonists are also described in more detail below:
  • X is selected from the group consisting of,
  • Yi is a bond or divalent linking group containing 1 to 9 atoms ;
  • Y2 and Y3 are divalent linking groups independently selected from -CH2-, -0-, and -S-;
  • Z is an Acidic Group
  • Rl is C ] _-C ] _o alkyl, aryl, C3-C10 cycloalkyl, C 2 -C 10 alkenyl, C 2 -C ⁇ 0 alkynyl, Cg-C 2 o aralkyl, C -C 2 o alkaryl, C ⁇ -C ⁇ o haloalkyl, C5-C20 aryloxy, or C ⁇ -C ⁇ o alkoxy;
  • R2 is hydrogen, halogen, C ⁇ -C ⁇ o haloalkyl, C ⁇ -C ⁇ o alkoxy, C ⁇ -C]_o alkyl, C3-C8 cycloalkyl, Acidic Group, or - (CH2) ⁇ _7 (Acidic Group) ;
  • R3 is hydrogen, halogen, C ⁇ -C ⁇ o alkyl, aryl, C]_-C ⁇ o haloalkyl, C]_-C]_o alkoxy, C ⁇ -C ⁇ o aryloxy, C3-C8 cycloalkyl;
  • R4 is C1-C4 alkyl, C3-C4 cycloalkyl,
  • n 0, 1, 2, 3, 4, 5, or 6;
  • Preferred LTB4 Antagonists include the following:
  • a "substituted heterocyclic radical” is preferably
  • Preferred LTB4 compounds of the invention include those wherein X is a heterocyclic radical selected from the group consisting of substituents represented by the following structural formulae:
  • RIO is a radical selected from hydrogen or c l _c 4 alkyl
  • Rll is a radical selected from hydrogen, halo, C ⁇ -C ⁇ o alkyl, haloalkyl, C ⁇ -C ⁇ o alkoxy, aryl, or C -C20 aryloxy.
  • Preferred RIO groups are hydrogen, methyl, or phenyl.
  • any of the above heterocyclic radicals illustrated by structural formulae may attach to the diphenyl leukotriene antagonist of formulae (I) by any monovalent bond originating on a suitable carbon or nitrogen atom in its ring structure.
  • the pyrrole radical may attach to the diphenyl molecule by a single bond originating at any carbon atom or any nitrogen atom which has less than three bonds in the heterocyclic ring; Location of attachment bond for pyrrole,
  • a preferred form of the substituent X is a fused bicyclic radical wherein a carbocyclic group is fused to two adjacent carbon atoms of the five membered heterocyclic radical, for example:
  • X substituents are the heterocyclic radicals
  • the heterocyclic radical X of Formula (I) does not include 3-bromo-l, 2 , 4 thiadiazole since the LTB4 antagonist activity of compounds containing this radical is considered too low to be an aspect of this invention.
  • Y ] _ is a bond or divalent linking group containing 1 to 9 atoms independently selected from carbon, hydrogen, sulfur, nitrogen, and oxygen;
  • Preferred LTB4 compounds of the invention include those wherein Y ⁇ is a divalent linking group selected from the group consisting of substituents represented by the following formulae: -o-
  • R13 is hydrogen, methyl, or ethyl
  • R13 is hydrogen, methyl, or ethyl
  • the above divalent groups may be used in their forward or reversed positions.
  • the most preferred divalent Y ⁇ substituent is the group
  • the Y2 and Y3 substituents are preferably selected from -S- and -0- .
  • Y2 and Y3 are the group
  • Z is the Acidic Group as previously defined, Preferred is an acidic group selected from the following:
  • R12 is C]_-C]_o alkyl, aryl, C -C20 alkaryl, or C -C20 aralkyl .
  • Preferred R12 groups are represented by the formulae:
  • Preferred Group 2 of Z substituent (symbol, "PG2-Z") : Highly preferred are the acidic groups; -5- tetrazolyl,
  • N-acyl sulfonamide, -SO3H, and carboxyl N-acyl sulfonamide, -SO3H, and carboxyl .
  • n 1.
  • a preferred Rl group is methyl, ethyl, n-propyl, isopropyl, n-butyl, sec-butyl, and 2-propenyl; with n- propyl being most preferred.
  • R2 and R3 groups are those wherein R2 and R3 groups
  • R3 are independently selected from hydrogen or methyl, ethyl, methoxy, ethoxy, halo, or -CF3 ; with R2 and R3 both being hydrogen as most preferred. III 0.
  • Preferred Group 1 of R4 substituent symbol, "PG1-R4":)
  • Preferred R4 substituents are ethyl, propyl, and isopropyl .
  • R-Table is used to select combinations of general and preferred groupings of the variables Rl , R2 , R3 and R4 for substitution in formula (I), as follows:
  • R14 describes a substituent combinatorial choice for Formula (I) wherein Rl is selected from the preferred set of variables, "PG1-R1", that is, methyl, ethyl, n-propyl, isopropyl, n-butyl, sec-butyl, and 2-propenyl; the R2 substituent is selected from the preferred set of variables, "PG1-R2", that is, hydrogen or methyl, ethyl, methoxy, ethoxy, halo, or -CF3 ; the variable R3 has the scope defined in the generic formula (I) , and the substituents suitable for R4 are selected from the preferred group, "PG1-R4" having the preferred set of variables, ethyl, propyl, and isopropyl.
  • Rl is selected from the preferred set of variables, "PG1-R1", that is, methyl, ethyl, n-propyl, isopropyl, n-butyl, sec-but
  • any of the individual 16 combinations of the R substituents depicted in the R-Table may be used in combination with any of the 27 individual combinations of Y substituents depicted in the Y-Table, which may be used with any of the 24 combinations of XZn substituents depicted in the XZn-Table.
  • the substituent combination choice "R07, Y21, XZn03" defines substituent set selections for a subset of formula (I) useful in the practice of the invention.
  • X2 is a heterocyclic radical selected from,
  • R21 is ethyl, 2-propen-l-yl, 3-propen-l-yl, n-propyl, iso-propyl, n-butyl, sec-butyl, or tert-butyl;
  • R22 is hydrogen, n-butyl, sec-butyl, flouro, chloro, -CF3, or tert-butyl.
  • Z2 is carboxyl, tetrazolyl, N-sulfonamidyl .
  • LTB4 antagonists are represented by the following structural formulae:
  • the salts of the above diphenyl LTB4 antagonists of the invention are an additional aspect of the invention.
  • the compounds of the invention possess an Acidic Group (s) and at these sites various salts may be formed which are more water soluble and/or physiologically suitable than the parent compound in its acid form.
  • Representative pharmaceutically acceptable salts include but are not limited to, the alkali and alkaline earth salts such as lithium, sodium, potassium, calcium, magnesium, aluminum and the like. Sodium salts are particularly preferred. Salts are conveniently prepared from the free acid by treating the acid form in solution with a base or by exposing the acid to an ion exchange resin.
  • the (Acidic Group) of the Z of Formula (I) may be selected as -CO2H and salts may be formed by reaction with appropriate bases (e.g., NaOH, KOH) to yield the corresponding sodium or potassium salt.
  • salts include the relatively non-toxic, inorganic and organic base addition salts of compounds of the present invention, for example, ammonium, quaternary ammonium, and amine cations, derived from nitrogenous bases of sufficient basicity to form salts with the LTB4 antagonist compounds of this invention (see, for example, S. M. Berge, et al . , "Pharmaceutical Salts," J. Phar. Sci., 66: 1-19 (1977)).
  • Certain compounds of the invention may possess one or more chiral centers and may thus exist in optically active forms. All such stereoisomers as well as the mixtures thereof are intended to be included in the invention. If a particular stereoisomer is desired, it can be prepared by methods well known in the art, for example, by using stereospecific reactions with starting materials which contain the asymmetric centers and are already resolved or, alternatively, by methods which lead to mixtures of the stereoisomers and subsequent resolution by known methods.
  • a racemic mixture may be reacted with a single enantiomer of some other compound. This changes the racemic form into a mixture of diastereomers . Then, because the diastereomers have different melting points, different boiling points, and different solubilities, they can be separated by conventional means, such as crystallization.
  • Prodrugs are derivatives of the compounds of Formulae (A), (I) and (II), supra., which have chemically or metabolically cleavable groups and become by hydrolysis or under physiological conditions the compounds of the invention which are pharmaceutically active in vivo.
  • Prodrugs include acid derivatives well known to practitioners of the art, such as, for example, esters prepared by reaction of the parent acidic compound with a suitable alcohol, or amides prepared by reaction of the parent acid compound with a suitable amine. Simple aliphatic or aromatic esters derived from acidic groups pendent on the compounds of this invention are preferred prodrugs. In some cases it is desirable to prepare double ester type prodrugs such as (acyloxy) alkyl esters or ( (alkoxycarbonyl) oxy) alkyl esters. Particularly
  • esters as prodrugs are methyl, ethyl, propyl, isopropyl, n-butyl, isobutyl, tert-butyl, morpholinoethyl, and N,N-diethylglycolamido.
  • esters of carboxylic acids are preferred prodrugs of the compounds of the invention (viz., the compounds of the invention).
  • Methyl ester prodrugs may be prepared by reaction of the acid form of a compound of formula (I) in a medium such as methanol with an acid or base esterification catalyst
  • Ethyl ester prodrugs are prepared in similar fashion using ethanol in place of methanol.
  • N,N-diethylglycolamido ester prodrugs may be prepared by reaction of the sodium salt of a compound of Formula (I) (in a medium such as dimethylformamide) with 2-chloro-N,N- diethylacetamide (available from Aldrich Chemical Co., Milwaukee, Wisconsin USA; Item No. 25,099-6).
  • Morpholinylethyl ester prodrugs may be prepared by reaction of the sodium salt of a compound of Formula (I) (in a medium such as dimethylformamide) 4- (2- chloroethyl)morpholine hydrochloride (available from Aldrich Chemical Co., Milwaukee, Wisconsin USA, Item No. C4, 220-3).
  • LTB4 antagonists include compounds of Formula A, Formula (I), or Formula (II) or the specific compounds of sections IIIR and HIS shown above by structural formula; wherein the acid, salt and prodrug derivatives thereof are respectively selected from: carboxylic acid, sodium salt, and ester prodrug.
  • IV. Method of Making the Compounds of the Invention General reaction schemes (not represented to be specific Examples) applicable for synthesis of the LTB4 antagonist compounds represented by formula (I) are set out below. Numerous literature references and Chemical Abstract registry numbers (e.g., RN 152609-60-4) are supplied as additional aids for preparing reagents used in practicing the synthesis schemes of the invention.
  • Example (1) a 4-substituted oxazole LTB4 receptor antagonist:
  • Known chloride (26) may be alkylated with benzyl bromide to provide chloride (28) .
  • Reaction with known ester (30) catalyzed by a suitable base, provides acetophenone (32).
  • Oxidation with bis (trifluoroacetoxy) iodobenzene gives alpha- hydroxy ketone (34), that may be cyclized with triflic anhydride and formamide to give the 4-substituted oxazole (36) .
  • Debenzylation with boron trifluoride etherate and ethanethiol gives oxazole (38), that is hydrolyzed and protonated to provide Example (1) .
  • Scheme 2 The following scheme illustrates a process for making Example (2), a 5 (4) -substituted imidazole LTB4 receptor antagonist: Scheme 2
  • the trimethylsilyl enol ether of acetophenone (32) is formed and treated with N-chlorosuccinimide followed by tetra-n- butylammonium fluoride to provide the chloroketone (40) .
  • Treatment of (40) with 2-benzyl-2-thiopseudourea and base provides imidazole (42), that is treated with boron trifluoride etherate and ethanethiol to give imidazole (44) .
  • Hydrolysis and protonation provide Example (2) as the hydrochloride salt.
  • Example (3) a 4-substituted thiazole LTB4 receptor antagonist:
  • Chloroketone (40) is treated with thioformamide and magnesium carbonate to give thiazole (46) , that is debenzylated with boron trifluoride etherate and ethanethiol giving thiazole (48) .
  • Hydrolysis and protonation provides Example (3) .
  • enone (50) Treatment of acetophenone (32) with N,N-dimethylformamide dimethyl acetal gives enone (50) , that may be hydrolyzed, protonated, and then heated with hydrazine hydrate to provide pyrazole (52). Debenzylation of the resulting pyrazole with boron trifluoride etherate and ethanethiol gives Example (4) .
  • Known phenol (30) is alkylated with known chloride (58) to give aryl bromide (60).
  • Heating (62) with trimethylsilyl azide provides triazole (64), that is debenzylated with boron trifluoride etherate and ethanethiol to give triazole (66). Hydrolysis and protonation provides Example (6) .
  • Example (8) a 5-substituted 1, 2 , 4-thiadiazole LTB4 receptor antagonist :
  • piperidinium salt (122) Treatment with piperidine makes piperidinium salt (122) .
  • piperidinium salt (122) By the teaching of Ikeda, infra, (the disclosure of which is incorporated herein by reference) treatment of (122) with 2- chloropyridinium methyl iodide followed by azide ion will give the 1, 2 , 3 , 4-thiatriazole (124).
  • Debenzylation with boron trifluoride etherate and ethanethiol, followed by hydrolysis and protonation, will provide the product of Example (18) .
  • Alkyne (62) is to be treated with trithiazyl trichloride by the method of Thomas et . al . (infra., the disclosure of which is incorporated herein by reference) to provide thiadiazole (132) .
  • Debenzylation with boron trifluoride etherate and ethanethiol, followed by hydrolysis and protonation, will provide the product of Example (20) .
  • Scheme 21 The following scheme illustrates a process for making Example (21), a 2-substituted 1, 3 , 4-thiadiazole LTB4 receptor antagonist :
  • Thiophene (114) may be reduced in the presence of triethylsilane and trifluoroacetic acid by the method of Kursanov et . al . (infra., the disclosure of which is incorporated herein by reference) to provide the thiophane (142) . Hydrolysis and protonation will provide the product of Example (24) .
  • PdCl 2 (dppf) (-15 mg) were added and heating continued at 110 °C for 20 h.
  • the mixture was cooled to room temperature, concentrated in vacuo, diluted with methylene chloride, and filtered down a short plug of silica gel with 20% ethyl acetate/80% hexane. The filtrate was concentrated in vacuo.
  • the resulting colorless oil was dissolved in methylene chloride (4 mL) , cooled to 0 °C, and treated with iodotrimethylsilane (0.40 mL, 2.7 mmol). The resulting mixture was allowed to warm to room temperature and stirred for 18 h.
  • the aqueous layer was acidified with concentrated hydrochloric acid and the resulting solution extracted with ethyl acetate.
  • the ethyl acetate layer was washed once with water, once with saturated sodium chloride solution, dried (sodium sulfate), filtered, and concentrated in vacuo.
  • the resulting colorless oil was dissolved in diethyl ether and treated with 1 N aqueous sodium hydroxide solution (1.72 mL) .
  • the resulting biphasic mixture was diluted with chloroform and concentrated in vacuo. Diethyl ether was added and the mixture concentrated in vacuo.
  • the resulting white foam was dried in vacuo at room temperature for 60 h to provide 0.78 g (84%) of the title compound: mp 67-71 °C.
  • compositions of the present invention are a combination of therapeutically effective amounts of the leukotriene (LTB4) antagonists, noted above, and a therapeutically effective amount of an anti-cancer agent or anti-cancer agents.
  • the composition may be formulated with common excipients, diluents or carriers, and compressed into tablets, or formulated elixirs or solutions for convenient oral administration or administered by intramuscular intravenous routes.
  • the compounds can be administered transdermally and maybe formulated as sustained relief dosage forms and the like.
  • the anti-cancer agents are formulated independently of the leukotriene (LTB4) antagonists and are administered separately.
  • the anti- cancer agents may be formulated with common excipients, diluents or carriers and administered by intravenous infusion.
  • the anti-cancer agents may be formulated into liquids suitable for oral administration.
  • Anti-cancer agents may also be compressed into tablets and administered orally. If the anti-cancer agents and the leukotrienes are administered separately, the anti-cancer agents may be administered before, after or during the administration of the leukotriene (LTB4) antagonists. If the anti-cancer agents are administered separately from the leukotrienes (LTB4) antagonists, they must be administered within a therapeutically effective interval.
  • the method of treating a human patient according to the present invention includes both the administration of the combination of leukotriene (LTB4) antagonists and an anti- cancer agent as well as the separate administration of the leukotriene (LTB4) antagonists and the anti-cancer agent.
  • the leukotriene (LTB4) antagonists are formulated into formulations which may be administered by the oral and rectal routes, topically, parenterally, e.g., by injection and by continuous or discontinuous intra-arterial infusion, in the form of, for example, tablets, lozenges, sublingual tablets, sachets, cachets, elixirs, gels, suspensions, aerosols, ointments, for example, containing from 1 to 10% by weight of the active compound in a suitable base, soft and hard gelatin capsules, suppositories, injectable solutions and suspensions in physiologically acceptable media, and sterile packaged powders adsorbed onto a support material for making injectable solutions.
  • formulations which may be administered by the oral and rectal routes, topically, parenterally, e.g., by injection and by continuous or discontinuous intra-arterial infusion, in the form of, for example, tablets, lozenges, sublingual tablets, sachets, cachets, e
  • compositions may be provided in dosage unit form, preferably each dosage unit containing from about 5 to about 500 mg (from about 5 to 50 mg in the case of parenteral or inhalation administration, and from about 25 to 500 mg in the case of oral or rectal administration) of a compound of Formula I or Formula II.
  • Dosages from about 0.5 to about 300 mg/kg per day, preferably 0.5 to 20 mg/kg, of active ingredient may be administered although it will, of course, readily be understood that the amount of the compound or compounds of Formula I actually to be administered will be determined by a physician, in the light of all the relevant circumstances including the condition to be treated, the choice of compound to be administered and the choice of route of administration and therefore the above preferred dosage range is not intended to limit the scope of the present invention in any way.
  • the formulations useful for separate administration of the leukotriene (LTB4) antagonists will normally consist of at least one compound selected from the compounds of Formula I and Formula II mixed with a carrier, or diluted by a carrier, or enclosed or encapsulated by an ingestible carrier in the form of a capsule, sachet, cachet, paper or other container or by a disposable container such as an ampoule.
  • a carrier or diluent may be a solid, semi-solid or liquid material which serves as a vehicle, excipient or medium for the active therapeutic substance.
  • diluents or carrier which may be employed in the pharmaceutical compositions of the present invention are lactose, dextrose, sucrose, sorbitol, mannitol, propylene glycol, liquid paraffin, white soft paraffin, kaolin, fumed silicon dioxide, microcrystalline cellulose, calcium silicate, silica, polyvinylpyrrolidone, cetostearyl alcohol, starch, modified starches, gum acacia, calcium phosphate, cocoa butter, ethoxylated esters, oil of theobroma, arachis oil, alginates, tragacanth, gelatin, syrup, methyl cellulose, polyoxyethylene sorbitan monolaurate, ethyl lactate, methyl and propyl hydroxybenzoate, sorbitan trioleate, sorbitan sesquioleate and oleyl alcohol and propellants such as trichloromonofluoromethane, dichlorodifluoromethane and dich
  • a lubricant may be incorporated to prevent sticking and binding of the powdered ingredients in the dies and on the punch of the tableting machine.
  • a lubricant may be employed for instance aluminum, magnesium or calcium stearates, talc or mineral oil.
  • Preferred pharmaceutical forms of the present invention are capsules, tablets, suppositories, injectable solutions, creams and ointments.
  • formulations for inhalation application such as an aerosol, and for oral ingestion.
  • the pharmaceutical composition of the invention comprises as essential ingredients: (a) an LTB4 antagonist, and
  • composition of the invention is prepared in injectable form it is a composition comprising as ingredients: (a) an LTB4 antagonist,
  • an injectable liquid carrier (c) an injectable liquid carrier.
  • Pharmaceutically acceptable carriers are those well known in the medical arts, such as sterile water, sterile water containing saline, and sterile water containing sugars and/or saline.
  • the essential ingredients (a) an LTB4 antagonist and (b) anti-cancer compound are present in the formulation in such proportion that a dose of the formulation provides a pharmaceutically effective amount of each ingredient to the patient being treated.
  • the weight ratio of LTB4 antagonist to anti-cancer agent 1:100 to 100 to 1, preferable from 10:1 to 1:10 and most preferable from 1:4 to 4:1.
  • formulation examples may employ as active compounds any of the leukotriene (LTB4) antagonists noted above.
  • LTB4 leukotriene
  • the examples are illustrative only and are not intended to limit the scope of the invention in any way.
  • Hard gelatin capsules are prepared using the following ingredients :

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Abstract

The therapeutic combinations of leukotriene (LTB4) inhibitors and anti-cancer agents are disclosed. A method of treating cancer using leukotriene (LTB4) inhibitors in conjunction with anti-cancer agents is also disclosed.

Description

ONCOLYTIC COMBINATIONS FOR THE TREATMENT OF CANCER
CROSS REFERENCE
This application claims priority from United States Provisional Patent Application No. 60/164,713 filed 11 November 1999; the entire disclosure of which is incorporated herein by reference.
FIELD OF THE INVENTION
This invention relates to a method of treating cancer with anti-cancer agents. More specifically, it relates to the use of anti-cancer agents, in conjunction with leukotriene (LTB4) antagonists which enhance the effectiveness of the anti-cancer agents.
BACKGROUND OF THE INVENTION
Leukotriene B4 (LTB4) is a proinflammatory lipid which has been implicated in the pathogenesis of psoriasis, arthritis, chronic lung diseases, acute respiratory distress syndrome, shock, asthma, inflammatory bone diseases and other inflammatory states characterized by the infiltration and activation of polymorphonuclear leukocytes and other proinflammatory cells. Thus activated, the polymorphonuclear leukocytes liberate tissue-degrading enzymes and reactive chemicals causing the inflammation. US Patent 5,462,954 discloses phenylphenol leukotriene antagonists which are useful in the treatment of psoriasis, arthritis, chronic lung diseases, acute respiratory distress syndrome, shock, asthma, inflammatory bone diseases and other inflammatory states characterized by the infiltration and activation of polymorphonuclear leukocytes and other proinflammatory cells. US Patent 5,910,505 discloses that certain phenylphenol leukotriene B4 (LTB4) antagonists are useful as agents for the treatment of oral squamous cell carcinoma. US Patent 5,543,428 discloses a group of phenylphenol leukotriene antagonists that have the property of reversing multi-drug resistance in tumor cells. The use of the leukotriene antagonist will reverse the drug resistance of resistant tumor cells to vinblastine, vincristine, vindesine, navelbine, daunorubicin, doxorubicin, mitroxantrone, etoposide, teniposide, mitomycin-C, actinomycin-D, taxol, topotecan, mithramycin, colchicine, puromycin, podophylotoxin, emetine, gramicidin-D, and valinomycin.
BRIEF SUMMARY OF THE INVENTION
This invention provides compositions and methods useful for treating cancers, which are not multi-drug resistant. The compositions of the present invention include anti- cancer agents in combination with leukotriene (LTB4) antagonists of formula A, formula I and formula II. Surprisingly, we have found that the combination of certain anti-cancer agents with leukotriene (LTB4) antagonists is highly effective in treating cancers which are not multi- drug resistant. DETAILED DESCRIPTION OF THE INVENTION
I. Definitions:
The term, "Acidic Group" means an organic group which when attached as the "Z" substituent of formula (I) or the "Z2" substituent of formula (II) acts as a proton donor capable of hydrogen bonding. An illustrative acidic group is carboxyl .
The term, "Active Ingredient" refers both to certain anti-cancer agents defined below and also leukotriene B4 antagonist compounds generically described by formula A as well as diphenyl leukotriene B4 antagonist compounds generically described by formula A, formula I and formula II or the list of specific diphenyl compounds disclosed, infra., as well as a combination of a anti-cancer agent and a leukotriene B4 antagonist described by formula A or formula I or II, and the salts, solvates, and prodrugs of such compounds .
The term, "alkenyl" means a monovalent radical of the generic formula CnH2n such as ethenyl, n-propenyl, isopropeneyl, n-butenyl, isobutenyl, 2-butenyl, and 3-butenyl .
The term, "alkyl" by itself or as part of another substituent means, unless otherwise defined, a straight or branched chain monovalent hydrocarbon radical such as methyl, ethyl, n-propyl , isopropyl, n-butyl, tertiary butyl, sec-butyl, n-pentyl, and n-hexyl . The term, "alkaryl" means an aryl radical substituted with an alkyl or substituted aryl group, for example:
In the term, "C5-C20 alkaryl" the numerical subscripts refer to the total number of carbon atoms in the radical.
The term, "C5-C20 aralkyl" means an alkyl radical substituted with an aryl or substituted aryl group, for example :
In the term, "C5-C20 aralkyl" the numerical subscripts refer to the total number of carbon atoms in the radical .
The term, "carbocyclic group" refers to a five, six, seven, or eight membered saturated, unsaturated or aromatic ring containing only carbon and hydrogen (e.g., benzene, cyclohexene, cyclohexane, cyclopentane) .
The term, "cycloalkyl" means a carbocyclic non- aromatic monovalent radical such as cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, and cyclooctyl . The term, "halo" means fluoro, chloro, bromo, or iodo,
The term, "heterocyclic radical (s) " refers to a radical having a saturated, unsaturated or aromatic five membered substituted or unsubstituted ring containing from 1 to 4 hetero atoms .
The terms, "mammal" and "mammalian" include human.
The term, "N-sulfonamidyl" means the radical
where R12 is C]_-Cιo alkyl, aryl, C1-C6 alkyl substituted aryl, C5-C20 alkaryl, or C5-C20 aralkyl.
The term, "substituted alkyl" means an alkyl group further substituted with one or more radical (s) selected from halo, C1-C alkyl, aryl, benzyl, C2-C5 alkenyl, C2-C6 alkynyl, C3-C8 cycloalkyl, alkoxy, C1-C haloalkyl (e.g. , -CF3) .
The term, "substituted aryl" means an aryl group further substituted with one or more radical (s) selected from halo, C1-C alkyl, aryl, benzyl, C2-C alkenyl, C2-C5 alkynyl, C3-C8 cycloalkyl, C]_-Cs alkoxy, C1-C haloalkyl (e.g. , -CF3) .
The term, "tetrazolyl" refers to an acidic group represented by either of the formulae:
The term "therapeutically effective interval" is a period of time beginning when one of either (a) the anti- cancer agent or (b) the LTB4 antagonist is administered to a mammal and ending at the limit of the anti-cancer beneficial effect in treating cancer of (a) or (b) . Typically, the anti-cancer agents and the leukotriene (LTB4) antagonist are administered within 24 hours of each other, more preferably within 4 hours and most preferably within 1 hour.
The phrase "therapeutically effective combination", used in the practice of this invention, means administration of both (a) the anti-cancer agent and (b) the LTB4 antagonist, either simultaneously or separately.
The types of cancers which may be treated with the compositions of the present invention include: Breast Carcinoma, Bladder Carcinoma, Colorectal Carcinoma, Esophageal Carcinoma, Gastric Carcinoma, Germ Cell Carcinoma e.g. Testicular Cancer, Gynecologic Carcinoma, Lymphoma - Hodgkin's, Lymphoma - Non-Hodgkin' s, Malignant Melanoma, Multiple Myeoma, Neurologic Carcinoma, Brain Cancer, Non-Small Cell Lung Cancer, Pancreatic Carcinoma, Prostate Carcinoma, Ewings Sarcoma, Osteosarcoma, Soft Tissue Sarcoma, Pediatric Malignancies and the like.
The anti cancer agents which may be used include:
ALKYLATING AGENTS: Busulfan, Carboplatin, Carmustine, Cisplatin, Cyclophosphamide, Dacarbazine, Ifosfamide, Lomustine, Streptozocin, Oxaliplatin, Temozolomide;
ANTIBIOTICS : Bleomycin, Dactinomycin, Daunorubicin, Doxorubicin, Idarubicin, Mitomycin-C, Plicamycin, Cryptophycin;
ANTIMETABOLITES : Cytarabine, Floxuridine, Fludarabine, 5- Fluorouracil, Hydroxyurea, 6-Mercaptopurine, Methotrexate, Thioguanine; Capecitabine;
BIOLOGICALS : Aldesleukin, Interferon Alfa-2A, Interleukin- 2, Interleukin-12 (recombinant), Interferon Alfa-2B (recombinant), Interferon Alfa-n3, Interferon Gamma-IB, Herceptin interleukin-2 , interleukin-12;
HORMONAL AGENTS: Aminoglutethimide, Anastrozole, Flutamide, Goserelin, Leuprolide, Megestrol, Mitotane, Tamoxifen;
NITROGEN MUSTARD DERIVATIVES: Chlorambucil , Estramustine, Mechlorethamine, Melphalan, Thiotepa; PLANT ALKALOIDS: Docetaxel, Etoposide, Irinotecan HCl, Paclitaxel, Teniposide, Topotecan, Vinblastine, Vincristine, Vinorelbine;
OTHERS: Altretamine, Amifostine Asparaginase-Escheriα ia coli strain, BCG Live (Intravesical) , Cladribine, Leucovorin, Levamisole, Mitoxantrone, Pegaspargase, Pentostatin, Procarbazine, and the like.
The anti-cancer agents may be used alone or in combinations of one or more anti-cancer agents. When used in combination, the anti-cancer agents may be administered at the same time, sequentially or in more complicated regimens where the agents may be administered alternately. Such combinations and dosing regimens are well known to those skilled in the art. The anti-cancer agents may be formulated with common excipients, diluents or carriers, and compressed into tablets, or formulated elixirs or solutions for convenient oral administration or administered by intramuscular intravenous routes. The compounds can be administered transdermally and may be formulated as sustained relief dosage forms and the like.
The compositions of the present invention are a combination of therapeutically effective amounts of the leukotriene (LTB4) antagonists, noted above, and a therapeutically effective amount of an anti-cancer agent. The composition may be formulated with common excipients, diluents or carriers, and compressed into tablets, or formulated elixirs or solutions for convenient oral administration or administered by intramuscular intravenous routes. The compounds can be administered transdermally and may be formulated as sustained relief dosage forms and the like.
In another embodiment, the invention relates to a method of treating a patient suffering from a non-multi-drug resistant cancerous condition which comprises the separate administration of a therapeutically effective amount of the leukotriene (LTB4) antagonists, and the anti-cancer agent. When administered separately, the leukotriene (LTB4) antagonists, and the anti-cancer agent may be administered on a different schedule. One may be administered before the other as long as the time between the two administrations falls within a therapeutically effective interval. Therapeutically effective interval is a period of time beginning when one of either (a) the leukotriene (LTB4) antagonists or (b) the anti-cancer agent is administered to a human and ending at the limit of the beneficial effect in the treatment of cancer of the combination of (a) and (b) .
The methods of administration of the leukotriene LTB4 antagonist and the anti-cancer agent may vary. Thus, one agent may be administered orally, while the other is administered intravenously. It is possible that one of the products may be administered as a continuous infusion while the other is provided in discreet dosage forms. It is particularly important that the anti-cancer drug be given in the manner known to optimize its performance.
The leukotriene (LTB4) antagonists useful in the present invention include those given in formula A.
Formula A
or a pharmaceutically acceptable base addition salt thereof, wherein:
Rl' is C1-C5 alkyl, C2-C5 alkenyl, C2-C5 alkynyl, C1-C4 alkoxy, (C1-C4 alkyl) thio, halo, or R2 ' -substituted phenyl; each R2 ' and R3 ' are each independently hydrogen, halo, hydroxy, C1-C4 alkyl, C1-C4 alkoxy, (C1-C4 alkyl) - (0) g S- , trifluoromethyl, or di- (C1-C3 alkyl)amino; X' is -0-, -S-, -C(=0), or -CH2-; Y' is -O- or -CH2-; or when taken together, -X'-Y'- is -CH=CH- or -C≡C- ; Z' is a straight or branched chain Ci-Cirj alkylidenyl; A' is a bond, -0-, -S-, -CH=CH- , or -CRaRjb-, where Ra and Rj are each independently hydrogen, C1-C5 alkyl, or R7 ' - substituted phenyl, or when taken together with the carbon atom to which they are attached form a C4-C8 cycloalkyl ring;
R4 ' is Rg or taken from one of the following formulae:
wherein: each Rg is independently -COOH, 5-tetrazolyl, CON(R9 ) 2. or -CONHSO2R10; each R7 is hydrogen, C1-C4 alkyl, C2-C5 alkenyl, C2-C5 alkynyl, benzyl, methoxy, -W-Rδ, -T-G-R6, (C1-C4 alkyl )-T- (C1-C4 alkylidenyl) -0-, or hydroxy;
R8 is hydrogen or halo; each R9 is independently hydrogen, phenyl, or C1-C4 alkyl, or when taken together with the nitrogen atom form a morpholino, piperidino, piperazino, or pyrrolidino group;
Rχθ is C1-C4 alkyl or phenyl;
RH is R2, -W-Rg, or -T-G-Rg; each W is a bond or a straight or branched chain divalent hydrocarbyl radical of one to eight carbon atoms; each G is a straight or branched chain divalent hydrocarbyl radical of one to eight carbon atoms; each T is a bond, -CH2-, -0-, -NH-, -NHCO-, -C(=0)-, or (0)g S-;
K is -C(=0)- or -CH(OH)-; each q is independently 0, 1, or 2 ; p is 0 or 1; and t is 0 or 1; provided when X is -0- or -S-, Y is not -0-; provided when A is -0- or -S-, R4 is not R6 ; and provided W is not a bond when p is 0.
More preferred compounds of Formula A are those wherein R4 ' is selected from the following formulae:
An even more preferred compound is that wherein R4'is:
Preferred compounds or pharmaceutically acceptable acid salt derivatives thereof are those wherein said compound selected from the group (A) to (KKKK) consisting of:
A) 2-Methyl-2- (lH-tetrazol-5-yl) -7- (2-ethyl-4- (4-fluorophenyl) -5-hydroxyphenoxy) heptane ;
B) 2-Methyl-2- (lH-tetrazol-5-yl) -7- (2-ethyl-4- ( 3-fluorophenyl ) -5-hydroxyphenoxy) heptane;
C) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- ( 4- dimethylaminocarbonylbutyloxy) phenyl ) propion ic acid;
D) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionic acid;
E) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy)propoxy) -6- (4- carboxybutyloxy) phenyl) propionic acid; F) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- methoxyphenyl) propionic acid; G) 3-(2-(3-(2-Ethyl-4-(4-fluorophenyl)-5- hydroxyphenoxy) propoxy) -6- (4- (lH-tetrazol-5- yl ) butyloxy) phenyl ) propionic acid;
H) Methyl 3- (2- (4- (2-ethyl-4- (4-fluorophenyl) - 5-hydroxyphenoxy) - ( 1- butenyl) ) phenyl) propionate;
I) 3- (2- (4- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) - (1-butenyl ) ) phenyl ) propionic acid;
J) 3- (2- (4- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) butyl ) phenyl ) propionic acid; K) 3-(2-(4-(2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) butyl ) -6- methoxyphenyl) propionic acid;
L) Methyl 3- (2- (3- (2-ethyl-4- (4-fluorophenyl) - 5-hydroxyphenoxy) propoxy) -6- hydroxyphenyl ) propionate;
M) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- hydroxyphenyl) propionic acid;
N) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- (4- butyloxy) phenyl) propionic acid;
0) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- (4- methylthiobutyloxy) phenyl) propionic acid; P) 3- (2- (3- (2,4-Di- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- ( 4- carboxybutoxy) phenyl) propionic acid;
Q) 6-Methyl-6- (lH-tetrazol-5-yl) -11- (2-ethyl-4- (4-fluorophenyl ) -5-hydroxyphenoxy) undecane; R) N,N-Dimethyl-3- (2- (3- (2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionamide; S) N-Methanesulfonyl-3-(2-(3- (2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionamide;
T) N-Phenylsulfonyl-3- (2- (3- (2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy)propoxy) phenyl) propionamide;
U) 3- (2- (3- (2-Butyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionic acid;
V) Ethyl 3- (2- (4- (2-ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) butyloxy) phenyl ) propionate; W) 3- (2-(4-(2-Ethyl-4-(4-fluorophenyl)-5- hydroxyphenoxy) butyloxy) phenyl ) propionic acid;
X) Methyl 3- (2- (3- (2-ethyl-4- (4-fluorophenyl) - 5-hyd oxyphenoxy) propoxy) -6- (4-
(methoxycarbonyl ) phenoxy) phenyl ) propionate ;
Y) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- (4- carboxyphenoxy) phenyl) propionic acid;
Z) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -4- (4- carboxyphenoxy) phenyl) propionic acid;
AA) 3, 3-Dimethyl-3-(2- (3- (2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) ropionic acid;
BB) 2-Methyl-2- (lH-tetrazol-5-yl) -3- (2- (3- (2- ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propane ;
CC) 2-Methyl-2- (lH-tetrazol-5-yl) -3-hydroxy-3- (2- (3- (2-ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propane ; DD) 3-(2-(3-(2-Bromo-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionic acid;
EE) 3- (2- (3- (2-Ethylthio-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionic acid;
FF) Methyl 3- (2-hydroxy-3- ( 4- methoxycarbonylbutyl ) -6- (3- (2-ethyl-4- (4- fluorophenyl ) -5- hydroxyphenoxy) propoxy) phenyl ) propionate;
GG) 5- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -8- (4-carboxybuty 1 ) dihydrocoumarin;
HH) 2-Phenyl-4-ethyl-5- [6- (2H-tetrazol-5-yl) -6- methylheptyloxy] phenol sodium salt; II) 2- (4-Methylphenyl) -4-ethyl-5-[6-methyl-6-
(2H-tetrazol-5-yl) heptyloxy] phenol disodium salt;
JJ) 2- (3-Methylphenyl) -4-ethyl-5- [ 6-methyl-6- (2H-tetrazol-5-yl) heptyloxy] phenol sodium salt;
KK) 2- (2-Methylphenyl) -4-ethyl-5- [6-methyl-6-
(2H-tetrazol-5-yl) heptyloxy] phenol disodium salt;
LL) 2- (4-Methoxyphenyl) -4-ethyl-5- [6-methyl-6- (2H-tetrazol-5-yl) heptyloxy] phenol sodium salt ;
MM) 2- (3-Methoxyphenyl) -4-ethyl-5- [6-methyl-6- (2H-tetrazol-5-yl)heptyloxy] phenol sodium salt; NN) 2- (4-Trifluoromethylphenyl) -4-ethyl-5- [6- methyl-6- (2H-tetrazol-5-yl) heptyloxy] phenol disodium salt;
00) 2- (3-Dimethylaminophenyl) -4-ethyl-5- [6- methyl-6- (2H-tetrazol-5-yl)heptyloxy] phenol disodium salt; PP) 3- (5- (6- (4-Phenyl-5-hydroxy-2- ethylphenoxy) propoxy) -2-carboxymethyl- 1,2,3,4 -tetrahydronaphthalen-l(2H) - one)propanoic acid; QQ) 3- (5- (6- (4- (4-Fluorophenyl) -5-hydroxy-2- ethylphenoxy) propoxy) -2-carboxymeth yl- 1,2, 3,4-tetrahydronaphthalen-l (2H) - one)propanoic acid; RR) 3- (4- (5- (4- (4-Fluorophenyl) -5-hydroxy-2- ethylphenoxy) propoxy) -2-carboxymeth yl-2 , 3- dihydroinden-1 (2H) -one)propanoic acid;
SS) 3,3-Dimethyl-5-(3- (2-carboxyethyl) -4- (3- (4- fluorophenyl) -5-hydroxy-2- ethylphenoxy) propoxy) phenyl ) -5-oxopentanoic acid;
TT) 7- [3- [ (5-Ethyl-2-hydroxy [1, 1 ' -biphenyl] -4- yl) oxy] propoxy] -3, 4-dihydro-8-propyl-2H-l- benzopyran-2-carboxylic acid;
UU) 8-Propyl-7- [3- [4- (4-fluorophenyl) -2-ethyl-5- hydroxyphenoxy] propoxy] -3 , 4-dihydro-2H-l- benzopyran-2-carboxylic acid;
W) 2-[3-[3-[ (5-Ethyl-2-hydroxy [1,1' -biphenyl] -
4-yl) oxy]propoxy] -2-propylphenoxy]propanoic acid;
WW) 2- (4-Chlorophenyl) -4-ethyl-5- [6-methyl-6-
(2H-tetrazol-5-yl) heptyloxy] phenol monosodium salt; XX) 2-(3,5-Dichlorophenyl)-4-ethyl-5- [6-methyl-
6- (2H-tetrazol-5-yl)heptyloxy] phenol monosodium salt; YY) 3-[2-[3-[ (5-Ethyl-2-hydroxy[l,l '-biphenyl] - 4-yl) oxy] propoxy] -l-dibenzofuran]propanoic acid disodium salt; ZZ) 7-Carboxy-9-oxo-3- [3- (2-ethyl-5-hydroxy-4- phenylphenoxy) ropoxy] -9H-xanthene-4- propanoic acid disodium salt monohydrate;
AAA) 2- [2-Propyl-3- [3- (2-ethyl-5-hydroxy-4- phenylphenoxy) propoxy] phenoxy]benzoic acid sodium salt hemihydrate;
BBB) 3-[3-(2-Ethyl-5-hydroxy-4- phenylphenoxy) propoxy] [1,1' -biphenyl ] -4- propanoic acid disodium salt monohydrate;
CCC) 5-Ethyl-4- [3- [2-propyl-3- [2- (2H-tetrazol-5- yl ) phenoxy] phenoxy] ropoxy] [1,1' -biphenyl ] - 2-ol disodium salt sesquihydrate;
DDD) 3-[4-[3-[ 3- (2-Ethyl-5-hydroxy-4- phenylphenoxy) propoxy] -9-oxo-9H- xanthene] ] propanoic acid sodium salt hemihydrate ;
EEE) 2-Fluoro-6- [2-propyl-3- [3- (2-ethyl-5- hydroxy-4- phenylphenoxy) propoxy] phenoxy] benzoic acid disodium salt;
FFF) 2- [2-Propyl-3- [3- [2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy] propoxy] phenoxy] benzoic acid sodium salt;
GGG) 3- [4- [7-Carboxy-9-oxo-3- [3- [2-ethyl-4- (4- fluorophenyl) -5-hydroxyphenoxy] propoxy] -9H- xanthene] ] propanoic acid disodium salt trihydrate;
HHH) 3- [4- [9-0x0-3- [3- [2-ethyl-4- (4- fluorophenyl) -5-hydroxyphenoxy] propoxy] -9H- xanthene] ] propanoic acid; III) 3- [2- [l-[2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy] propoxy] -4- (5-oxo-5- morpholinopentanamido) phenyl] propanoic acid; JJJ) 2-Fluoro-6- [2-propyl-3- [3- [2-ethyl-5- hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] phenoxy] benzoic acid disodium salt hydrate;
KKK) 4-Fluoro-2- [2-propyl-3- [3- [2-ethyl-5- hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] phenoxy] benzoic acid;
LLL) 2- [2-Propyl-3- [5- [2-ethyl-5-hydroxy-4- (4- fluorophenyl ) phenoxy] pentoxy] phenoxy] benzoic acid; MMM) 2- [2-Propyl-3-[4- [2-ethyl-5-hydroxy-4- (4- fluorophenyl ) phenoxy] butoxy] phenoxy] benzoic acid sesquihydrate;
NNN) 2- [2- (2-Methylproρyl) -3- [3- [2-ethyl-5- hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] phenoxy] benzoic acid;
000) 2- [2-Butyl-3- [3- [2-ethyl-5-hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] phenoxy] benzoic acid hydrate;
PPP) 2- [2- (Phenylmethyl) -3- [3- [2-ethyl-5-hydroxy- 4-(4- fluorophenyl ) phenoxy] propoxy] phenoxy] benzoic acid;
QQQ) 2- [2-Propyl-3- [3- [2-ethyl-5-hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] phenoxy] phenyla cetic acid;
RRR) 2- [2-Propyl-3- [3- [2-ethyl-5-hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] benzoyl ] benzoic acid;
SSS) 2- [ [2-Propyl-3- [3- [2-ethyl-5-hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] phenyl ] methyl ] b enzoic acid; TTT) 2-[2-Propyl-3-[3-[2-ethyl-4-(4- fluorophenyl) -5- hydroxyphenoxy] propoxy] thiophenoxy] benzoic acid; UUU) 2- [2-Propyl-3- [3- [2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy] propoxy] phenylsulfinyl ] benzoi c acid;
VW) 2- [2-Propyl-3- [3- [2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy] propoxy] phenylsulfonyl] benzoi c acid hydrate;
WWW) 5- [3- [2- (1-Carboxy) ethyl] -4- [3- [2-ethyl-4- ( 4-fluorophenyl ) -5- hydroxyphenoxy] propoxy] phenyl] -4-pentynoic acid disodium salt 0.4 hydrate;
XXX) 1-Phenyl-l- (lH-tetrazol-5-yl) -6- (2-ethyl-4- (4-fluorophenyl ) -5-hydroxyphenoxy) hexane ; YYY) 1- (4- (Carboxymethoxy) phenyl )-l-(lH-tetrazol-
5-yl) -6- (2-ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) hexane;
ZZZ) 1- (4- (DimethylaminocarbonyImethoxy) phenyl) - 1- (lH-tetrazol-5-yl) -6- (2-ethyl-4- (4- fluorophenyl ) -5-hydroxyphenoxy) hexane ;
AAAA) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) -E-propenoic acid;
BBBB) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl) -2-methyl-E- propenoic acid;
CCCC) 5- (2- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) ethyl ) -1H- tetrazole;
DDDD) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -4- (4- carboxybutyloxy) henyl) propionic acid;
EEEE) 5- [3- [4- (4-Fluorophenyl ) -2-ethyl-5- hydroxyphenoxy] propoxy] -3 , 4-dihydro-2H-l- benzopyran-2-one; FFFF) 3- (3-{3-[2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenyloxy] propoxy}phenyl ) propanoic acid;
GGGG) 3- (3-{3- [2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenyloxy] propoxy} -4-propylph enyl) propanoic acid sodium salt;
HHHH) 3- (4-{3- [2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenyloxy] propoxy} -3- propylphenyl ) propanoic acid;
IIII) 3- (3-{3- [2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenyloxy] propoxy} -2- propylphenyl) propanoic acid;
JJJJ) 3-{3-[3-(2-Ethyl-5- hydroxyphenyloxy) propoxy] -2 - propylphenyl }propanoic acid disodium salt; and
KKKK) 2- [3- [3- [2-Ethyl-5-hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] benzoyl] benzoic acid disodium salt hemihydrate.
These leukotriene (LTB4) antagonists are well known in the art, and are fully described in U.S. Patent 5,462,954, which is hereby specifically incorporated by reference for its disclosure of the methods of preparation of specific leukotriene B4 antagonists and compounds or formulations of the leukotriene antagonists which may be administered to patients. A preferred compound is 2- [2-propyl-3- [3- [2- ethy1-5-hydroxy-4- ( 4-f1ouropheny1 ) phenoxy] propoxy] phenoxy] benzoic acid which can also be named 2- [3- [3- (5-ethyl-4 ' - flouro-2-hydroxybiphen-4-yloxy) propoxy] -2- propylphhenoxy] benzoic acid, described in U.S. Patent 5,462,954 as example 66 and also shown below as Compound A (Formula B) :
Compound A (Formula B)
A second class of LTB4 antagonists to use as the essential co-agent in the compositions and practice of the method of this invention are those disclosed in copending provisional patent application, titled, "Heterocycle Substituted Diphenyl Leukotriene Antagonists" (inventor, Jason Scott Sawyer) containing 97 pages and identified as Eli Lilly and Company Docket No. B-13240), filed on November 11, 1999, and now Provisional patent Application Serial Number 60/164,786. The subject Heterocycle substituted diphenyl leukotriene antagonists are also described in more detail below:
II. Additional LTB4 Antagonists:
Additional LTB4 antagonists are described below which are novel heterocyclic substituted diphenyl compounds of formula (I)
wherein:
X is selected from the group consisting of,
(i) a five membered substituted or unsubstituted heterocyclic radical containing from 1 to 4 hetero atoms independently selected from sulfur, nitrogen or oxygen; or
(ii) a fused bicyclic radical wherein a carbocyclic group is fused to two adjacent carbon atoms of the five membered heterocyclic radical, (i) ;
Yi is a bond or divalent linking group containing 1 to 9 atoms ;
Y2 and Y3 are divalent linking groups independently selected from -CH2-, -0-, and -S-;
Z is an Acidic Group;
Rl is C]_-C]_o alkyl, aryl, C3-C10 cycloalkyl, C2-C10 alkenyl, C2-Cι0 alkynyl, Cg-C2o aralkyl, C -C2o alkaryl, Cχ-Cχo haloalkyl, C5-C20 aryloxy, or Cχ-Cχo alkoxy; R2 is hydrogen, halogen, Cχ-Cιo haloalkyl, Cι-Cχo alkoxy, Cχ-C]_o alkyl, C3-C8 cycloalkyl, Acidic Group, or - (CH2) ι_7 (Acidic Group) ;
R3 is hydrogen, halogen, Cχ-Cχo alkyl, aryl, C]_-Cχo haloalkyl, C]_-C]_o alkoxy, Cχ-Cχo aryloxy, C3-C8 cycloalkyl;
R4 is C1-C4 alkyl, C3-C4 cycloalkyl,
- (CH2) ι_7 (cycloalkyl) , C2-C4 alkenyl, C2-C4 alkynyl, benzyl, or aryl; and
n is 0, 1, 2, 3, 4, 5, or 6;
or a pharmaceutically acceptable salt, solvate, or prodrug derivative thereof.
III. Preferred LTB4 Antagonists include the following:
III A. Preferred X substituents :
A "substituted heterocyclic radical" is preferably
Substituted with from 1 to 3 groups independently selected from hydrogen, halo, Cι-Cχo alkyl, Cχ-Cιo haloalkyl, Cχ-Cχo alkoxy, aryl, or C -C20 aryloxy. Preferred Group 1 of X substituent (symbol, "PG1-X")
Preferred LTB4 compounds of the invention include those wherein X is a heterocyclic radical selected from the group consisting of substituents represented by the following structural formulae:
ι
where RIO is a radical selected from hydrogen or cl_c4 alkyl; and Rll is a radical selected from hydrogen, halo, Cι-Cχo alkyl, haloalkyl, Cχ-Cχo alkoxy, aryl, or C -C20 aryloxy. Preferred RIO groups are hydrogen, methyl, or phenyl. Moreover, any of the above heterocyclic radicals illustrated by structural formulae may attach to the diphenyl leukotriene antagonist of formulae (I) by any monovalent bond originating on a suitable carbon or nitrogen atom in its ring structure.
For example, the pyrrole radical may attach to the diphenyl molecule by a single bond originating at any carbon atom or any nitrogen atom which has less than three bonds in the heterocyclic ring; Location of attachment bond for pyrrole,
A preferred form of the substituent X is a fused bicyclic radical wherein a carbocyclic group is fused to two adjacent carbon atoms of the five membered heterocyclic radical, for example:
III B. Preferred Group 2 of X substituent (symbol, "PG2- X") :
Most preferred as the X substituents are the heterocyclic radicals;
or
III C. Excluded X substituents:
The heterocyclic radical X of Formula (I) does not include 3-bromo-l, 2 , 4 thiadiazole since the LTB4 antagonist activity of compounds containing this radical is considered too low to be an aspect of this invention.
Ill D. Preferred Y]_ substituents:
Y]_ is a bond or divalent linking group containing 1 to 9 atoms independently selected from carbon, hydrogen, sulfur, nitrogen, and oxygen;
Preferred Group 1 of Yi substituent (symbol, "PG1-Yχ")
Preferred LTB4 compounds of the invention include those wherein Y^ is a divalent linking group selected from the group consisting of substituents represented by the following formulae: -o-
O
c — — C
H2 H2
— c
H2
H2
H,
and
where R13 is hydrogen, methyl, or ethyl; The above divalent groups may be used in their forward or reversed positions. For example, the group;
may be positioned as either,
in the displayed fragment of formula (I) .
Ill E. Preferred Group 2 of Yi substituent (symbol, "PG2- Yi"):
The most preferred divalent Y^ substituent is the group;
-o-
III F. Preferred Group 1 of Y2 substituent (symbol, '"PG1- Y2") and Preferred Group 1 of Y3 substituent (symbol, "PG1- Y3") :
The Y2 and Y3 substituents are preferably selected from -S- and -0- .
III G. Preferred Group 2 of Y2 substituent (symbol, "PG2- Y2") and Preferred Group 2 of Y3 substituent (symbol, "PG2-
Y3") :
Most preferably both Y2 and Y3 are the group;
III H. Preferred Group 1 of Z substituent (symbol, "PG1-Z") :
Z is the Acidic Group as previously defined, Preferred is an acidic group selected from the following:
tetrazolyl,
-SO3H,
-OH or
where R12 is C]_-C]_o alkyl, aryl, C -C20 alkaryl, or C -C20 aralkyl . Preferred R12 groups are represented by the formulae:
III I. Preferred Group 2 of Z substituent (symbol, "PG2-Z") : Highly preferred are the acidic groups; -5- tetrazolyl,
N-acyl sulfonamide, -SO3H, and carboxyl .
III J. Preferred Group 3 of Z substituent (symbol, "PG3-Z") : Carboxyl is the most preferred Z substituent.
Ill K. Preferred Group 1 of n subscript variable (symbol, "PGl-n")
The most preferred integer values for the divalent linking group, -(CH2)n~ ' are n=l, n=2, and n=3.
Ill L. Preferred Group 2 of n subscript variable (symbol, "PG2-n")
The most preferred integer value of n for the divalent linking group, -(CH2)n- is n = 1.
III M. Preferred Group 1 of Rl substituent (symbol, "PG1- Rl") :
A preferred Rl group is methyl, ethyl, n-propyl, isopropyl, n-butyl, sec-butyl, and 2-propenyl; with n- propyl being most preferred.
III N. Preferred Group 1 of R2 substituent
(symbol, "PG1-R2")and Preferred Group 1 of R3 substituent
(symbol, "PG1-R3") :
Preferred R2 and R3 groups are those wherein R2 and
R3 are independently selected from hydrogen or methyl, ethyl, methoxy, ethoxy, halo, or -CF3 ; with R2 and R3 both being hydrogen as most preferred. III 0. Preferred Group 1 of R4 substituent (symbol, "PG1-R4":)
Preferred R4 substituents are ethyl, propyl, and isopropyl .
III P. Combinations of substituents of the compound of Formula ( I ) :
The substituents of formula (I) are defined as "Z", "X", "n", "Rl", "R2", "R3", "R4", "Yl", "Y2", and "Y3". Moreover, as described in the preceding section, within each of the defined substituents of Formula (I) are "preferred" and "most preferred" subgroups which define the variety of substituents to be used in the definition of LTB4 antagonists of the invention. These preferred subgroups are defined by designations such as "PG1-R4" as recited above. It is often advantageous to use combinations of preferred groups or combinations of preferred groups together with the general definition of variables given in Formula (I) . Suitable combinations of substituents are shown in the following three Tables (viz., R-Table, Y-Table & XZn-Table) .
The following R-Table is used to select combinations of general and preferred groupings of the variables Rl , R2 , R3 and R4 for substitution in formula (I), as follows:
R-Table
Thus, for example, the substituent combination, "R14" describes a substituent combinatorial choice for Formula (I) wherein Rl is selected from the preferred set of variables, "PG1-R1", that is, methyl, ethyl, n-propyl, isopropyl, n-butyl, sec-butyl, and 2-propenyl; the R2 substituent is selected from the preferred set of variables, "PG1-R2", that is, hydrogen or methyl, ethyl, methoxy, ethoxy, halo, or -CF3 ; the variable R3 has the scope defined in the generic formula (I) , and the substituents suitable for R4 are selected from the preferred group, "PG1-R4" having the preferred set of variables, ethyl, propyl, and isopropyl.
The following Y-Table is used to select broad and preferred groupings of the variables Yl, Y2 , and Y3 for substitution in formula (I), as follows:
Y-Table
The following XZn-Table is used to select broad and preferred groupings of the variables X, Z, and n for substitution in formula (I), as follows:
XZn-Table
How to Use the Tables :
Any of the individual 16 combinations of the R substituents depicted in the R-Table may be used in combination with any of the 27 individual combinations of Y substituents depicted in the Y-Table, which may be used with any of the 24 combinations of XZn substituents depicted in the XZn-Table. For example, the substituent combination choice "R07, Y21, XZn03" defines substituent set selections for a subset of formula (I) useful in the practice of the invention.
Ill Q. Additional preferred LTB4 antagonists are described by formula (II) :
wherein;
X2 is a heterocyclic radical selected from,
or
R21 is ethyl, 2-propen-l-yl, 3-propen-l-yl, n-propyl, iso-propyl, n-butyl, sec-butyl, or tert-butyl; and
R22 is hydrogen, n-butyl, sec-butyl, flouro, chloro, -CF3, or tert-butyl.
Z2 is carboxyl, tetrazolyl, N-sulfonamidyl . Preferred Compounds of the Invention:
III R. Specific compounds preferred as LTB4 antagonists are represented by the following structural formulae:
(CD
(C2)
(C3)
(C4)
(C5)
(C6)
(C7)
(C8)
(C9)
(CIO)
(Cll) :
(C12)
(C13)
(C14)
(C15)
(ci6:
(C17)
(C18)
(C19)
(C20) :
(C21)
( C23 ) :
and all acid, salt, solvate and prodrug derivatives thereof
III S. Highly Preferred LTB4 Antagonists are as follows:
and all acid, salt, solvate and prodrug derivatives thereof
The salts of the above diphenyl LTB4 antagonists of the invention, represented by formulae (A), (I) and (II) and the specific compounds set out by structural formulae in sections IIIR and HIS herein, are an additional aspect of the invention. The compounds of the invention possess an Acidic Group (s) and at these sites various salts may be formed which are more water soluble and/or physiologically suitable than the parent compound in its acid form. Representative pharmaceutically acceptable salts, include but are not limited to, the alkali and alkaline earth salts such as lithium, sodium, potassium, calcium, magnesium, aluminum and the like. Sodium salts are particularly preferred. Salts are conveniently prepared from the free acid by treating the acid form in solution with a base or by exposing the acid to an ion exchange resin. For example, the (Acidic Group) of the Z of Formula (I) may be selected as -CO2H and salts may be formed by reaction with appropriate bases (e.g., NaOH, KOH) to yield the corresponding sodium or potassium salt.
Included within the definition of pharmaceutically acceptable salts are the relatively non-toxic, inorganic and organic base addition salts of compounds of the present invention, for example, ammonium, quaternary ammonium, and amine cations, derived from nitrogenous bases of sufficient basicity to form salts with the LTB4 antagonist compounds of this invention (see, for example, S. M. Berge, et al . , "Pharmaceutical Salts," J. Phar. Sci., 66: 1-19 (1977)).
Certain compounds of the invention may possess one or more chiral centers and may thus exist in optically active forms. All such stereoisomers as well as the mixtures thereof are intended to be included in the invention. If a particular stereoisomer is desired, it can be prepared by methods well known in the art, for example, by using stereospecific reactions with starting materials which contain the asymmetric centers and are already resolved or, alternatively, by methods which lead to mixtures of the stereoisomers and subsequent resolution by known methods.
For example, a racemic mixture may be reacted with a single enantiomer of some other compound. This changes the racemic form into a mixture of diastereomers . Then, because the diastereomers have different melting points, different boiling points, and different solubilities, they can be separated by conventional means, such as crystallization.
Prodrugs are derivatives of the compounds of Formulae (A), (I) and (II), supra., which have chemically or metabolically cleavable groups and become by hydrolysis or under physiological conditions the compounds of the invention which are pharmaceutically active in vivo.
Derivatives of the compounds of this invention have activity in both their acid and base derivative forms, but the acid derivative form often offers advantages of solubility, tissue compatibility, or delayed release in a mammalian organism (see, Bundgard, H., Design of Prodrugs, pp. 7-9, 21-24, Elsevier, Amsterdam 1985) . Prodrugs include acid derivatives well known to practitioners of the art, such as, for example, esters prepared by reaction of the parent acidic compound with a suitable alcohol, or amides prepared by reaction of the parent acid compound with a suitable amine. Simple aliphatic or aromatic esters derived from acidic groups pendent on the compounds of this invention are preferred prodrugs. In some cases it is desirable to prepare double ester type prodrugs such as (acyloxy) alkyl esters or ( (alkoxycarbonyl) oxy) alkyl esters. Particularly
preferred esters as prodrugs are methyl, ethyl, propyl, isopropyl, n-butyl, isobutyl, tert-butyl, morpholinoethyl, and N,N-diethylglycolamido.
Esters of carboxylic acids are preferred prodrugs of the compounds of the invention (viz., the compounds of
Formula A, Formula I, Formula II and the specific compounds set out in Section IIIR and HIS, herein) .
Methyl ester prodrugs may be prepared by reaction of the acid form of a compound of formula (I) in a medium such as methanol with an acid or base esterification catalyst
(e.g., NaOH, H2SO4). Ethyl ester prodrugs are prepared in similar fashion using ethanol in place of methanol.
N,N-diethylglycolamido ester prodrugs may be prepared by reaction of the sodium salt of a compound of Formula (I) (in a medium such as dimethylformamide) with 2-chloro-N,N- diethylacetamide (available from Aldrich Chemical Co., Milwaukee, Wisconsin USA; Item No. 25,099-6).
Morpholinylethyl ester prodrugs may be prepared by reaction of the sodium salt of a compound of Formula (I) (in a medium such as dimethylformamide) 4- (2- chloroethyl)morpholine hydrochloride (available from Aldrich Chemical Co., Milwaukee, Wisconsin USA, Item No. C4, 220-3).
Preferred LTB4 antagonists include compounds of Formula A, Formula (I), or Formula (II) or the specific compounds of sections IIIR and HIS shown above by structural formula; wherein the acid, salt and prodrug derivatives thereof are respectively selected from: carboxylic acid, sodium salt, and ester prodrug. IV. Method of Making the Compounds of the Invention General reaction schemes (not represented to be specific Examples) applicable for synthesis of the LTB4 antagonist compounds represented by formula (I) are set out below. Numerous literature references and Chemical Abstract registry numbers (e.g., RN 152609-60-4) are supplied as additional aids for preparing reagents used in practicing the synthesis schemes of the invention.
REACTION SCHEMES FOR MAKING THE COMPOUNDS OF THE INVENTION
The following scheme illustrates a process for making Example (1), a 4-substituted oxazole LTB4 receptor antagonist:
Scheme 1
benzyl bromide, CSgCOg, DMF
(26) known compound: RN# 156005-61 -7
R. W. Harper et al., J. Med. Chem. 1994, 37(15), 2411
1
(28) KoCO,, Nal, 2-butanone
(38)
Known chloride (26) may be alkylated with benzyl bromide to provide chloride (28) . Reaction with known ester (30) , catalyzed by a suitable base, provides acetophenone (32). Oxidation with bis (trifluoroacetoxy) iodobenzene gives alpha- hydroxy ketone (34), that may be cyclized with triflic anhydride and formamide to give the 4-substituted oxazole (36) . Debenzylation with boron trifluoride etherate and ethanethiol gives oxazole (38), that is hydrolyzed and protonated to provide Example (1) .
Scheme 2 The following scheme illustrates a process for making Example (2), a 5 (4) -substituted imidazole LTB4 receptor antagonist: Scheme 2
(42)
(44)
The trimethylsilyl enol ether of acetophenone (32) is formed and treated with N-chlorosuccinimide followed by tetra-n- butylammonium fluoride to provide the chloroketone (40) . Treatment of (40) with 2-benzyl-2-thiopseudourea and base provides imidazole (42), that is treated with boron trifluoride etherate and ethanethiol to give imidazole (44) . Hydrolysis and protonation provide Example (2) as the hydrochloride salt.
Scheme 3
The following scheme illustrates a process for making Example (3), a 4-substituted thiazole LTB4 receptor antagonist:
Scheme 3
thioformamide, MgC03 dioxane
(46)
Chloroketone (40) is treated with thioformamide and magnesium carbonate to give thiazole (46) , that is debenzylated with boron trifluoride etherate and ethanethiol giving thiazole (48) . Hydrolysis and protonation provides Example (3) .
Scheme 4 The following scheme illustrates a process for making Example (4), a 5 (3) -substituted pyrazole LTB4 receptor antagonist:
Scheme 4
Treatment of acetophenone (32) with N,N-dimethylformamide dimethyl acetal gives enone (50) , that may be hydrolyzed, protonated, and then heated with hydrazine hydrate to provide pyrazole (52). Debenzylation of the resulting pyrazole with boron trifluoride etherate and ethanethiol gives Example (4) .
Scheme 5 The following scheme illustrates a process for making Example (5), a 5-substituted isoxazole LTB4 receptor antagonist:
Scheme 5
(50)
(56)
Treatment of enone (50) with hydroxylamine provides isoxazole (54), that is debenzylated with boron trifluoride etherate and ethanethiol to give isoxazole (56) . Hydrolysis and protonation provides Example (5) .
Scheme 6 The following scheme illustrates a process for making Example (6), a 5 (4) -substituted 1, 2 , 3-triazole LTB4 receptor antagonist :
Scheme 6
known compounds: RN 152609-60-4 152609-76-2
J. S. Sawyer et al. J. Med. Chem. 1995, 38, 4411
Known phenol (30) is alkylated with known chloride (58) to give aryl bromide (60). Treatment of (60) with tri-n- butylethynyltin and a palladium catalyst gives alkyne (62) . Heating (62) with trimethylsilyl azide provides triazole (64), that is debenzylated with boron trifluoride etherate and ethanethiol to give triazole (66). Hydrolysis and protonation provides Example (6) .
Scheme 7 The following scheme illustrates a process for making Example (7), a 1-substituted pyrrole LTB4 receptor antagonist:
Scheme 7
References for formation of 1-aryl substituted pyrroles: M. Mure and J. P. Klinman, J. Am. Chem. Soc. 1995, 117(34), 8698; Y. Lee et al. J. Am. Chem. Soc. 1996, 118(30), 7241 4-Ethylbenzene-l, 3-diol (68) is treated with potassium nitrosodisulfonate followed by 3-pyrroline and benzylbromide and a base to provide pyrrole (70) . Alkylation with 1- bromo-3-chloropropane gives chloride (72), that is used to alkylate phenol (30) to give pyrrole (74) . Debenzylation with boron trifluoride etherate and ethanethiol provides Example (7 ) .
Scheme 8
The following scheme illustrates a process for making Example (8), a 5-substituted 1, 2 , 4-thiadiazole LTB4 receptor antagonist :
Scheme 8
The palladium-catalyzed addition of 4 , 4 , 5 , 5-tetramethyl- [1, 3 , 2] dioxaborolane to bromide (60) gives boronic ester (76) . The palladium-catalyzed addition of 3-bromo-5-chloro- 1, 2 , 4-thiadiazole to (76) gives ester (78). Debenzylation with boron trifluoride etherate and ethanethiol, followed by hydrolysis and protonation, gives Example (8) .
Scheme 9 The following scheme illustrates a process for making Example (9), a 2-substituted thiophene LTB4 receptor antagonist:
Scheme 9
The palladium-catalyzed addition of boronic ester (76) to 2- bromothiophene, followed by debenzylation with boron trifluoride etherate and ethanethiol, provides thiophene (80) . Hydrolysis and salt formation provides Example (9) .
Scheme 10 The following scheme illustrates a process for making Example (10), a 4-substituted pyrazole LTB4 receptor antagonist:
SchemelO
(76)
(82)
The palladium-catalyzed addition of boronic ester (76) to 1- methyl-4-iodopyrazole provides pyrazole (82). Debenzylation with boron trifluoride etherate and ethanethiol, followed by hydrolysis and protonation, provides Example (10) .
Scheme 11 The following scheme illustrates a process for making Example (11), a 2-substituted thiazole LTB4 receptor antagonist:
Scheme 11
The palladium-catalyzed addition of boronic ester (76) to 2- bromothizaole provides thiazole (84) . Debenzylation with boron trifluoride etherate and ethanethiol gives thiazole (86). Hydrolysis and protonation provides Example (11).
Scheme 12 The following scheme illustrates a process for making Example (12), a 4-substituted isoxazole LTB4 receptor antagonist:
Scheme 12
(88)
The palladium-catalyzed addition of boronic ester (76) to 3 , 5-dimethyl-4-iodoisoxazole provides oxazole (88). Debenzylation with trimethylsilyl iodide, followed by hydrolysis and salt formation, provides Example (12).
Scheme 13
The following scheme illustrates a process for making Example (13), a 2-substituted furan LTB4 receptor antagonist:
Scheme 13
(60)
(92)
(94)
Debenzylation of bromide (60) with boron tribromide provides phenol (90), that is treated with tert-butyldimethylsilyl chloride and imidazole to give silyl ether (92). The palladium-catalyzed addition of (92) to furan-2-boronic acid provides furan (94) . Hydrolysis and salt formation gives Example (13) .
Scheme 14 The following scheme illustrates a process for making Example (14), a 3-substituted furan LTB4 receptor antagonist:
Scheme 14
(92)
The palladium-catalyzed addition of (92) to furan-3 -boronic acid provides furan (96) . Hydrolysis and salt formation gives Example (14) . Scheme 15 The following scheme illustrates a process for making Example (15), a 3-substituted tetrahydrofuran LTB4 receptor antagonist
Scheme 15
(100)
The palladium-catalyzed addition of bromide (60) to furan-3- boronic acid provides furan (98) . Hydrogenation over a palladium catalyst gives tetrahydrofuran (100) . Hydrolysis and salt formation gives Example (15).
Scheme 16
The following scheme illustrates a process for making Example (16), a 2-substituted pyrrolidine LTB4 receptor antagonist:
Scheme 16
Pd(PPh3)4, aq. Na2C03, THF
(60)
(106)
The palladium-catalyzed addition of bromide (60) to N-boc pyrrole-2-boronic acid provides pyrrole (102). Hydrogenation over a palladium catalyst gives pyrrolidine (104) . Hydrolysis and salt formation gives pyrrolidine (106). Treatment with hydrochloric acid provides Example (16) as the hydrochloride salt.
Scheme 17 The following scheme illustrates a process for making Example (17), a 3-substituted thiophene LTB4 receptor antagonist:
Scheme 17
(58) (108)
(112)
(114)
The palladium-catalyzed addition of bromide (58) to thiophene-3-boronic acid provides thiophene (108) . Alkylation of known phenol (110) with (108) catalyzed by base provides thiophene (112) . Debenzylation with boron tribromide gives thiophene (114) . Hydrolysis and protonation provide Example (17).
Scheme 18 The following scheme illustrates a process for making Example (18), a 5-substituted 1, 2 , 3 , 4-thiatriazole LTB4 receptor antagonist :
Scheme 18
2) CSjjCOa, BnBr, DMF
Reference for formation of dithioacids: N. C. Gonnella et al. Syn. Commun. 1979, 17
Reference for formation of 5-substituted 1 ,2,3,4-thiatriazoles from dithioacids: S. I. Ikeda et al., Synthesis 1990, 415 Phenol (30) is alkylated with l-bromo-3-chloropropane to give chloride (116), that is in turn to be treated with known aldehyde (118) and a base, followed by benzylation with benzyl bromide and a base, to provide aldehyde (120) . From aldehyde (120) is made the thioacetal by treatment with 1, 2-ethanedithiol . The resulting thioacetal is then to be treated with base to provide the thioacid. Treatment with piperidine makes piperidinium salt (122) . By the teaching of Ikeda, infra, (the disclosure of which is incorporated herein by reference) treatment of (122) with 2- chloropyridinium methyl iodide followed by azide ion will give the 1, 2 , 3 , 4-thiatriazole (124). Debenzylation with boron trifluoride etherate and ethanethiol, followed by hydrolysis and protonation, will provide the product of Example (18) .
Scheme 19 The following scheme illustrates a process for making Example (19), a 4-substituted 1, 2 , 3-thiadiazole LTB4 receptor antagonist:
Scheme 19
NH.NHCOOEt
(130)
Reference for 1 ,2,3-thiadiazole formation: E. W. Thomas et al., J. Med. Chem. 1985, 28, 442. Treatment of acetophenone (32) with ethyl carbazate will give the hydrazone (128) . Use of thionyl chloride by the method of Thomas et . al . (infra., the disclosure of which is incorporated herein by reference) will give an intermediate 1, 2 , 3-thiadiazole (130), that is to be debenzylated with boron trifluoride etherate and ethanethiol, then hydrolyzed and protonated to give the product of Example (19) .
Scheme 20 The following scheme illustrates a process for making Example (20), a 3-substituted 1, 2 , 5-thiadiazole LTB4 receptor antagonist :
Scheme 20
(62)
(132)
Reference for 1 ,2,5-thiadiazole formation: E. W. Thomas et al., J. Med. Chem. 1985, 28, 442.
Alkyne (62) is to be treated with trithiazyl trichloride by the method of Thomas et . al . (infra., the disclosure of which is incorporated herein by reference) to provide thiadiazole (132) . Debenzylation with boron trifluoride etherate and ethanethiol, followed by hydrolysis and protonation, will provide the product of Example (20) . Scheme 21 The following scheme illustrates a process for making Example (21), a 2-substituted 1, 3 , 4-thiadiazole LTB4 receptor antagonist :
Scheme 21
(134)
The palladium-catalyzed addition of boronic ester (76) to 2- bromo-1, 3 , 4-thiadiazole will provide ester (134). Debenzylation with boron trifluoride etherate and ethanethiol, followed by hydrolysis and protonation, will provide the product of Example (21) .
Scheme 22 The following scheme illustrates a process for making Example (22), a 5-substituted isothiazole LTB4 receptor antagonist:
Scheme 22
(136)
The palladium-catalyzed addition of bromide (58) to 3- methylisothiazole-5-boronic acid will provide isothiazole (136) . Alkylation of phenol (30) with (136) catalyzed by base will provide isothiazole (138). Debenzylation with boron trifluoride etherate and ethanethiol, followed by hydrolysis and protonation, will provide the product of Example (22) .
Scheme 23 The following scheme illustrates a process for making Example (23), a 2-substituted oxazole LTB4 receptor antagonist:
Scheme 23 1 Br
(140)
The palladium-catalyzed addition of boronic ester (76) to 2- bromooxazole will provide oxazole (140) . Debenzylation with boron trifluoride etherate and ethanethiol, followed by hydrolysis and protonation, will provide the product of Example (23) .
Scheme 24 The following scheme illustrates a process for making Example (24), a 3-substituted thiophane LTB4 receptor antagonist:
Scheme 24
Et3SiH, TFA, benzene
(114)
(142)
Reference for formation of tetrahydrothiophenes: D. N. Kursanov et al. Tetrahedron 1975, 31, 311
Thiophene (114) may be reduced in the presence of triethylsilane and trifluoroacetic acid by the method of Kursanov et . al . (infra., the disclosure of which is incorporated herein by reference) to provide the thiophane (142) . Hydrolysis and protonation will provide the product of Example (24) . V. PREPARATIVE EXAMPLES 1 TO 17:
Example 1
Preparation of 2-{3- [3- (2-Ethyl-5-hydroxy-4-oxazol-4-yl- phenoxy)propoxy] -2-propyl-phenoxy}benzoic acid.
known compound: R # 156005-61-7
R. W. Harper et al . , J. Med. Chem. 1994, 37 (15) , 2411-20
A. Preparation of 1- [2-benzyloxy-4- (3-chloropropoxy) -5- ethylphenyl] ethanone.
A mixture of 1- [2-hydroxy-4- (3-chloropropoxy) -5- ethylphenyl] ethanone (26.1 g, 102 mmol), cesium carbonate (33.4 g, 103 mmol), and benzyl bromide (12.2 ml, 103 mmol), in N,N-dimethylformamide (300 mL) was stirred for 5 h at room temperature. The mixture was diluted with ethyl acetate and washed four times with water. The organic layer was dried (sodium sulfate) , filtered, and concentrated in vacuo . The resulting oil was triturated with ethyl acetate and hexane, allowed to stand for 18 h, then cooled at 0 °C for 3 h. The resulting precipitate was collected via vacuum filtration to provide 24.3 g (69%) of the title compound as
1 white crystals: mp 60-61 °C. H NMR (CDC13) δ 7.68 (s, 1H) , 7.40 (m, 5H) , 6.48 (s, 1H) , 5.17 (s, 2H) , 4.13 (t, J = 6 Hz, 2H) , 3.75 (t, J = 6 Hz, 2H) , 2.56 (s, 3H) , 2.55
(q, J = 7 Hz, 2H) , 2.26 (quintet, J = 6 Hz, 2H) , 1.16 (t, J
+ = 7 Hz, 3H) ; TOF MS ES exact mass calculated for
C20H24ClO3 (p+1) : m/z = 347.1414. Found: 347.1402; IR
(CHC13,
-1 cm ) 1659, 1602, 1266.
Anal. Calcd for C20 H23clo3 : C' 69-26; H, 6.68. Found: C, 69.30; H, 6.52.
known compound: RN# 152609-76-2 J. S. Sawyer et al., J. Med. Chem. 1995, 38, 4411
B. Preparation of 2-{3- [3- (4-acetyl-5-benzyloxy-2- ethylphenoxy)propoxy] -2-propyl-phenoxy}benzoic acid methyl ester.
A mixture of 1- [2-benzyloxy-4- (3-chloropropoxy) -5- ethylphenyl] ethanone (7.27 g, 21.0 mmol) and sodium iodide (3.14 g, 23.1 mmol) in 2-butanone (100 mL) was heated at reflux for 18 h. The mixture was cooled to room temperature, filtered, and concentrated in vacuo. The residue was dissolved in N,N-dimethylformamide (100 mL) and treated with 2- (3-hydroxy-2-propylphenoxy) benzoic acid methyl ester (6.0 g, 21 mmol) and potassium carbonate (3.2 g, 23 mmol) at room temperature for 15 h. The mixture was diluted with ethyl acetate and washed four times with water and once with saturated sodium chloride solution. The organic layer was dried (sodium sulfate) , filtered, and concentrated in vacuo. Chromatography (silica gel, 10% ethyl acetate/90% hexane) of the residue provided 9.2 g
1 (72%) of the title compound as a colorless oil. H MR
(CDC13) δ 7.88 (d, J = 9 Hz, 1H) , 7.69 (s, 1H) , 7.38 (m,
6H) , 7.12 (d, J = 8 Hz, 1H) , 7.07 (d, J = 8 Hz, 1H) , 6.80 (d, J = 8 Hz, 1H) , 6.67 (d, J = 8 Hz, 1H) , 6.50 (s, 1H) , 6.44 (d, J = 9 Hz, 1H) , 5.14 (s, 2H) , 4.20 (m, 4H) , 3.83 (s, 3H) , 2.65 (t, J = 7 Hz, 2H) , 2.57 (q, J = 7 Hz, 2H) , 2.56 (s, 3H) , 2.32 (quintet, J = 6 Hz, 2H) , 1.55 (hextet, J = 7
Hz, 2H) , 1.15 (t, J = 8 Hz, 3H) , 0.90 (t, J = 7 Hz, 3H) ; IR
-1 (CHCI3, cm ) 2965, 1726, 1602, 1461.
Anal. Calcd for C37H40O7 : C, 74.48; H, 6.76. Found: C, 74.39; H, 6.77.
C. Preparation of 2- (3-{3- [5-benzyloxy-2-ethyl-4- (2- hydroxyacetyl)phenoxy]propoxy} -2-propylphenoxy)benzoic acid methyl ester.
A mixture of 2- {3- [3- (4-acetyl-5-benzyloxy-2- ethylphenoxy) propoxy] -2-propyl-phenoxy}benzoic acid methyl ester (5.31 g, 8.89 mmol) and water (10 mL) in acetonitrile (50 mL) was treated with trifluoroacetic acid (1.4 mL) , 18 mmol) and [bis (trifluoroacetoxy) iodo.benzene (7.65 g, 17.8 mmol) . The resulting mixture was heated at reflux for 4 h then concentrated in vacuo. The residue was dissolved in methylene chloride and washed once with water. The aqueous layer was extracted twice with fresh portions of methylene chloride. The combined organic layers were washed three times with saturated sodium bicarbonate solution, once with saturated sodium chloride solution, dried (sodium sulfate) , filtered, and concentrated in vacuo. Chromatography (silica gel, 20% ethyl acetate/80% hexane) of the residue provided
1 1.68 g (31%) of the title compound as a brown oil. H MR
(CDC13) δ 7.92 (s, 1H) , 7.88 (d, J = 9 Hz, 1H) , 7.40 (m, 6H) , 7.12 (d, J = 9 Hz, 1H) , 7.05 (d, J = 9 Hz, 1H) , 6.79
(d, J = 8 Hz, 1H) , 6.66 (d, J = 8 Hz, 1H) , 6.50 (s, 1H) ,
6.43 (d, J = 8 Hz, 1H) , 5.15 (s, 2H) , 4.65 (s, 2H) , 4.22 (m,
4H) , 3.83 (s, 3H) , 2.65 (m, 4H) , 2.34 (quintet, J = 6 Hz,
2H) , 1.55 (hextet, J = 7 Hz, 2H) , 1.17 (t, J = 8 Hz, 3H) ,
+ 0.89 (t, J = 8 Hz, 3H) ; TOS MS ES exact mass calculated
for C37H4108 (ρ+1) : m/z = 613.2801. Found: 613.2833.
D. Preparation of 2-{3- [3- (5-bβnzyloxy-2-ethyl-4-oxazol-4- yl-phenoxy)propoxy] -2-propylphenoxy}benzoic acid methyl ester.
To a solution of 2- (3-{3- [5-benzyloxy-2-ethyl-4- (2- hydroxyacetyl ) phenoxy] propoxy} -2-propylphenoxy) benzoic acid methyl ester (1.39 g, 2.27 mmol) in methylene chloride (20 mL) cooled to -78 °C was added triflie anhydride (0.57 mL, 3.4 mmol) and 2,6-lutidine (0.40 mL, 3.4 mmol). The resulting mixture was stirred for 1 h then poured into ether and water. The organic layer was separated and washed once with saturated sodium chloride solution, dried (sodium sulfate), filtered, and concentrated in vacuo. The residue was dissolved in a 2:1 mixture of formamide/N,N- dimethylformamide (9 mL) and heated at 120 °C in a sealed tube for 4 h. The mixture was cooled to room temperature and diluted with ethyl acetate. The mixture was washed four times with water, once with saturated sodium chloride solution, dried (sodium sulfate) , filtered, and concentrated in vacuo. Chromatography (silica gel, 10% ethyl acetate/90% hexane) of the residue provided 89 mg (6%) of the title
1 product as a colorless oil. H NMR (CDC13) δ 7.92 (s, 1H) ,
7.85 (s, 1H) , 7.83 (m, 2H) , 7.35 (m, 6H) , 7.03 (d, J - 8 Hz,
1H) , 7.00 (d, J = 8 Hz, 1H) , 6.73 (d, J = 8 Hz, 1H) , 6.62
(d, J = 8 Hz, 1H) , 6.52 (s, 1H) , 6.35 (d, J = 8 Hz, 1H) ,
5.07 (s, 2H) , 4.14 (m, 4H) , 3.76 (s, 3H) , 2.61 (m, 4H) , 2.26
(quintet, J = 6 Hz, 2H) , 1.48 (hextet, J = 7 Hz, 2H) , 1.15
(t, J = 8 Hz, 3H) , 0.84 (t, J = 8 Hz, 3H) .
E. Preparation of 2-{3- [3- (2-ethyl-5-hydroxy-4-oxazol-4-yl- phenoxy)propoxy] -2-propylphenoxy}benzoic acid methyl ester.
To a solution of 2- {3- [3- (5-benzyloxy-2-ethyl-4-oxazol-4-yl- phenoxy) propoxy] -2-propylphenoxy}benzoic acid methyl ester (89 mg, 0.14 mmol) in ethanethiol (2 mL) was treated with boron trifluoride etherate (0.27 mL, 2.2 mmol) at room temperature for 4 h. The solution was poured into ether and washed once with water, once with saturated sodium bicarbonate solution, once with saturated sodium chloride solution, dried (sodium sulfate) , filtered, and concentrated in vacuo. Chromatography (silica gel, 15% ethyl acetate/85% hexane) of the residue provided 34 mg (45%) of the title
1 product as a light brown oil. H MR (CDC13) δ 7.99 (d, J =
1 Hz, 1H) , 7.90 (d, J = 1 Hz, 1H) , 7.88 (dd, J = 8, 2 Hz, 1H) , 7.38 (t, J = 7 Hz, 1H) , 7.15 (s, 1H) , 7.10 (d, J = 9 Hz, 1H) , 7.06 (d, J = 9 Hz, 1H) , 6.81 (d, J = 9 Hz, 1H) , 6.70 (d, J = 9 Hz, 1H) , 6.52 (s, 1H) , 6.44 (d, J = 9 Hz, 1H) , 4.20 (m, 4H) , 3.83 (s, 3H) , 2.65 (t, J = 8 Hz, 2H) , 2.58 (q, J = 8 Hz, 2H) , 2.33 (quintet, J = 6 Hz, 2H) , 1.55 (hextet, J = 7 Hz, 2H) , 1.17 (t, J = 8 Hz, 3H) , 0.91 (t, J = 8 Hz, 3H) ; MS ES+ m/e = 532 (p + 1) .
F. Preparation of 2-{3- [3- (2-ethyl-5-hydroxy-4-oxazol-4-yl- phenoxy)propoxy] -2-propylphenoxy}benzoic acid. To a solution of 2- {3- [3- (2-ethyl-5-hydroxy-4-oxazol-4-yl- phenoxy) propoxy] -2-propylphenoxy}benzoic acid methyl ester (89 mg, 0.14 mmol) in methanol (2 mL) was added 1 M lithium hydroxide solution (0.28 mL) and the resulting mixture warmed at 60 °C for 3.5 h. The mixture was cooled to room temperature and concentrated in vacuo. The aqueous residue was diluted with water and the pH adjusted to -4. The mixture was extracted three times with methylene chloride. The combined organic extracts were dried (sodium sulfate), filtered, and concentrated in vacuo to provide 27 mg (92%)
1 of the title compound as a yellow solid. H MR (DMSO-dg) δ 12.83 (bs, 1H) , 10.12 (bs, 1H) , 8.39 (s, 1H) , 8.25 (s, 1H) , 7.78 (dd, J = 8, 1 Hz, 1H) , 7.64 (s, 1H) , 7.47 (t, J = 8 Hz, 1H) , 7.16 (m, 2H) , 6.80 (t, J = 8 Hz, 2H) , 6.56 (s, 1H) , 6.35 (d, J = 8 Hz, 1H) , 4.20 (t, J = 6 Hz, 2H) , 4.12 (t, J = 6 Hz, 2H) ; 2.54 (m, 4H) , 2.24 (quintet, J = 6 Hz, 2H) , 1.43 (hextet, J = 8 Hz, 2H) , 1.10 (t, J = 8 Hz, 3H) , 0.80 (t, J = 8 Hz, 3H) ; TOF MS ES exact mass calculated
for C30H32NO7 (p+1) : m/z = 518.2179. Found: 518.2206; IR
-1 (KBr, cm ) 2961, 1696, 1460, 1222.
Anal. Calcd for C30H31NO7: C, 69.62; H, 6.04; N, 2.71. Found: C, 68.71; H, 5.82; N, 2.65.
Example 2 Preparation of 2- (3-{3- [2-Ethyl-5-hydroxy-4- (3H-imidazol-4- yl)phenoxy]propoxy} -2-propy1-phenoxy)benzoic acid hydrochloride.
A. Preparation of 2- (3- {3- [5-benzyloxy-4- (2-chloroacetyl) - 2-ethylphenoxy]propoxy} -2-propylphenoxy)benzoic acid methyl ester.
To a solution of 2- {3- [3- (4-acetyl-5-benzyloxy-2- ethylphenoxy) propoxy] -2-propyl-phenoxy}benzoic acid methyl ester (3.04 g, 5.09 mmol) in tetrahydrofuran (50 mL) cooled to -78 °C was added a solution of 1 M lithium hexamethyldisilazide in tetrahydrofuran (11.2 mL, 11.2 mmol) portion wise. After stirring for 20 min, trimethylsilyl chloride (2.6 mL, 20 mmol) was added and the mixture warmed to 0 °C and stirred for 30 min. The mixture was evaporated in vacuo and the residue dissolved in hexane. The resulting solution was filtered and concentrated in vacuo. The residue was dissolved in tetrahydrofuran (50 mL) , cooled to 0 °C, and treated with N-chlorosuccinimide (750 mg, 5.6 mmol) . The mixture was warmed to room temperature and stirred for 30 min, then heated at reflux for 2 h. The mixture was cooled to room temperature and treated with water (4 mL) and a solution of 1 N tetra-n-butylammonium fluoride in tetrahydrofuran (6 mL) . After stirring for 15 min the mixture was diluted in ether and washed once with water, once with saturated sodium chloride solution, dried (sodium sulfate), filtered, and concentrated in vacuo. Chromatography (silica gel, 10% ethyl acetate/90% hexane) of the residue provided 1.94 g (60%) of the title compound as a
1 white solid. H NMR (CDC13) δ 7.89 (d, J = 8 Hz, 1H) , 7.77
(s, 1H) , 7.40 (m, 6H) , 7.12 (d, J = 9 Hz, 1H) , 7.06 (d, J = 8 Hz, 1H) , 6.80 (d, J = 8 Hz, 1H) , 6.66 (d, J = 8 Hz, 1H) , 6.49 (s, 1H) , 6.43 (d, J = 8 Hz, 1H) , 5.15 (s, 2H) , 4.68 (s, 2H) , 4.20 (q, J = 6 Hz, 4H) , 3.82 (s, 3H) , 2.65 (t, J = 7 Hz, 2H) , 2.59 (q, J = 7 Hz, 2H) , 2.32 (quintet, J = 6 Hz,
2H) , 1.54 (hextet, J = 8 Hz, 2H) , 1.16 (t, J = 8 Hz, 3H) ,
+ 0.89 (t, J = 7 Hz, 3H) ; TOF MS ES exact mass calculated
for C37H40ClO7 (p+1) : m/z = 631.2463. Found: 631.2470; IR
-1 (CHCI3, cm ) 2964, 1720, 1603, 1461. Anal. Calcd for C37H39C107: C, 70.41; H, 6.23. Found: C, 70.04; H, 5.97.
B. Preparation of 2- (3-{3- [5-benzyloxy-4- (2-benzylsulfanyl- SH-imidazol^-yl) -2-ethyl-phenoxy]propoxy}-2- propylphenoxy)benzoic acid methyl ester.
A mixture of 2- (3- {3- [5-benzyloxy-4- (2-chloroacetyl) -2- ethylphenoxy] ropoxy} -2-propylphenoxy) benzoic acid methyl ester (800 mg, 1.27 mmol), 2-benzyl-2-thiopseudourea hydrochloride (313 mg, 1.52 mmol), sodium iodide (77 mg, 0.51 mmol), and potassium carbonate (700 mg, 5.06 mmol) in N,N-dimethylformamide (20 mL) was treated at 80 °C for 6 h. The mixture was cooled, diluted with diethyl ether, and washed once with water. The organic layer was dried (sodium sulfate), filtered, and concentrated in vacuo. Chromatography (silica gel, 30% ethyl acetate/70% hexane) of the residue provided 376 mg (40%) of the title compound as a yellow amorphous solid. H NMR (CDCI3) δ 7.89 (d, J = 8 Hz,
1H) , 7.36 (m, 9H) , 7.20 (m, 5H) , 7.21 (d, J = 9 Hz, 1H) , 7.06 (d, J = 8 Hz, 1H) , 6.79 (d, J = 8 Hz, 1H) , 6.67 (d, J = 8 Hz, 1H) , 6.55 (s, 1H) , 6.43 (d, J = 8 Hz, 1H) , 5.07 (s, 2H) , 4.21 (t, J = 6 Hz, 2H) , 4.18 (t, J = 6 Hz, 2H) , 4.10 (s, 2H) , 3.83 (s, 3H) , 2.63 (m, 4H) , 2.31 (quintet, J = 6
Hz, 2H) , 1.55 (hextet, J = 7 Hz, 2H) , 1.18 (t, J = 8 Hz,
+ 3H) , 0.90 (t, J = 7 Hz, 3H) ; TOF MS ES exact mass
calculated for C45H47N20gS (p+1) : m/z = 743.3155. Found:
-1 743.3142; IR (CHCI3, cm ) 2963, 1720, 1602, 1453.
Anal. Calcd for C45H46N20gS: C, 72.75; H, 6.24; N, 3.77.
Found: C, 72.69; H, 6.17; N, 3.56
C. Preparation of 2- (3-{3- [4- (2-benzylsulfanyl-3ff-imidazol- 4-yl) -2-ethyl-5-hydroxyphenoxy]propoxy} -2- propylphenoxy)benzoic acid methyl ester. A solution of 2- (3-{3- [5-benzyloxy-4- (2-benzylsulfanyl-3ff- imidazol-4-yl) -2-ethyl-phenoxy] propoxy} -2- propylphenoxy) benzoic acid methyl ester (360 mg, 0.49 mmol) in ethanethiol (7 mL) was treated with boron trifluoride etherate at room temperature for 3.5 h. The mixture was diluted with diethyl ether and water. The organic layer was separated and washed with saturated sodium bicarbonate solution, dried (sodium sulfate) , filtered, and concentrated in vacuo. Chromatography (silica gel, 20% ethyl acetate/80% hexane) of the residue provided 154 mg (48%) of the title
1 compound as an orange oil. H NMR (CDCI3) δ 7.85 (d, J = 8
Hz, 1H) , 7.36 (t, J = 7 Hz, 1H) , 7.20 (m, 7H) , 7.12 (s, 1H) , 7.05 (m, 3H) , 6.79 (d, J = 8 Hz, 1H) , 6.65 (d, J = 8 Hz, 1H) , 6.54 (s, 1H) , 6.41 (d, J = 8 Hz, 1H) , 4.20 (s, 2H) , 4.17 (m, 4H) , 3.82 (s, 3H) , 2.62 (t, J = 8 Hz, 2H) , 2.54 (q, J = 7 Hz, 2H) , 2.30 (quintet, J = 6 Hz, 2H) , 1.53 (hextet, J
= 8 Hz, 2H) , 1.14 (t, J = 7 Hz, 3H) , 0.89 (t, J = 8 Hz, 3H) ;
+ TOF MS ES exact mass calculated for C38H41N206S (p+1) : m/z = 653.2685. Found: 653.2669.
Anal. Calcd for C38H40N2O6S: C, 69.92; H, 6.18; N, 4.29. Found: C, 69.44; H, 6.25; N, 3.99.
D. Preparation of 2- (3-{3- [2-ethyl-5-hydroxy-4- (3H- imidazol-4-yl)phenoxy]propoxy} -2-propyl-phenoxy)benzoic acid hydrochloride.
A solution of 2- (3- {3- [4- (2-benzylsulfanyl-3H-imidazol-4- yl) -2-ethyl-5-hydroxyphenoxy] propoxy} -2- propylphenoxy) benzoic acid methyl ester (154 mg, 0.235 mmol) in methanol (3 mL) was treated with 1 N lithium hydroxide solution at 60 °C for 3.5 h. The mixture was cooled to room temperature and concentrated in vacuo. The solution was diluted with water and adjusted to pH 4. The aqueous solution was extracted three times with methylene chloride. The combined organic layers were dried (sodium sulfate), filtered, and concentrated in vacuo. The residue was dissolved in ethanol (3 mL) and treated with 0.2 N sodium hydroxide solution (1 mL) and Raney nickel (75 mg) at 75 °C for 4 h. The mixture was cooled to room temperature, TM filtered through Celite , and the filtrate concentrated in vacuo. The residue was diluted with water and adjusted to pH 2 with 1 N hydrochloric acid. The resulting precipitate was collected via vacuum filtration to provide 27 mg (21%)
+ of the title compound. TOF MS ES exact mass calculated for C30H33N2O6 (p+1) : m/z = 517.2339. Found: 517.2340.
Example 3 Preparation of 2-{3- [3- (2-Ethyl-5-hydroxy-4-thiazol-4-yl- phenoxy)propoxy] -2-propyl-phenoxy}benzoic acid.
A. Preparation of 2-{3- [3- (5-benzyloxy-2-ethyl-4-thiazol-4- yl-phenoxy)propoxy] -2-propylphenoxy}benzoic acid methyl ester.
A mixture of 2- (3- {3- [5-benzyloxy-4- (2-chloroacetyl) -2- ethylphenoxy] propoxy} -2-propylphenoxy) benzoic acid methyl ester (500 mg, 0.792 mmol), thioformamide (20 mL, 8.0 mmol), and magnesium carbonate in dioxane (10 mL) was heated at reflux for 2 h. The mixture was cooled to room temperature and diluted with diethyl ether and 0.2 M sodium hydroxide solution. The organic layer was separated, washed with saturated sodium chloride solution, dried (sodium sulfate) , filtered, and concentrated in vacuo. Chromatography (silica gel, 10% ethyl acetate/90% hexane) of the residue provided
254 mg (50%) of the title compound as a colorless oil H
NMR (CDC13) δ 8.91 (s, 1H) , 8.11 (s, 1H) , 7.87 (dd, J = 8, 1
Hz, 1H) , 7.84 (d, J = 1 Hz, 1H) , 7.40 (m, 6H) , 7.08 (m, 2H) , 6.80 (d, J = 8 Hz, 1H) , 6.68 (d, J = 8 Hz, 1H) , 6.62 (s, 1H) , 6.43 (d, J = 8 Hz, 1H) , 5.16 (s, 2H) , 4.21 (t, J = 6 Hz, 4H) , 3.83 (s, 3H) , 2.68 (m, 4H) , 2.32 (quintet, J = 6 Hz, 2H) , 1.56 (hextet, J = 8 Hz, 2H) , 1.21 (t, J = 7 Hz,
3H) 0.90 (t, J = 7 Hz, 3H) ; TOF MS ES exact mass
calculated for C38H40NO6S (p+1) : m/z = 638.2576. Found:
-1 638.2579. IR (CHCI3, cm ) 2964, 1719, 1563, 1461.
B. Preparation of 2-{3- [3- (2-ethyl-5-hydroxy-4-thiazol-4- yl-phenoxy)propoxy] -2-propylphenoxy}benzoic acid methyl ester.
A solution of 2- {3- [3- (5-benzyloxy-2-ethyl-4-thiazol-4-yl- phenoxy) propoxy] -2-propyl-phenoxy}benzoic acid methyl ester (243 mg, 0.366 mmol) in ethanethiol (7 mL) was treated with boron trifluoride etherate at room temperature for 4 h. The mixture was diluted with diethyl ether, washed once with water, once with saturated sodium bicarbonate solution, dried (sodium sulfate), filtered, and concentrated in vacuo. Chromatography (silica gel, 15% ethyl acetate/85% hexane) of the residue provided 131 mg (65%) of the title compound as a
1 colorless oil. H NMR (CDC13) δ 8.88 (d, J = 1 Hz , 1H) ,
7.88 (dd, J = 8, 1 Hz, 1H) , 7.44 (d, J = 1 Hz, 1H) , 7.38 (m, 2H) , 7.08 (m, 2H) , 6.81 (d, J = 8 Hz, 1H) , 6.68 (d, J = 8
Hz, 1H) , 6.55 (s, 1H) , 6.43 (d, J = 8 Hz, 1H) , 4.21 (t, J = 6 Hz, 4H) , 3.83 (s, 3H) , 2.63 (m, 4H) , 2.33 (quintet, J = 6
Hz, 2H) , 1.56 (hextet, J = 8 Hz, 2H) , 1.19 (t, J = 8 Hz,
+ 3H) , 0.91 (t, J = 7 Hz, 3H) ; TOF MS ES exact mass
calculated for C31H34N06S (p+1) : m/z = 548.2107. Found: 548.2085.
C. Preparation of 2-{3- [3- (2-ethyl-5-hydroxy-4-thiazol-4- yl-phenoxy)propoxy] -2-propylphenoxy}benzoic acid.
A solution of 2-{3- [3- (2-ethyl-5-hydroxy-4-thiazol-4-yl- phenoxy) propoxy] -2-propylphenoxy}benzoic acid methyl ester (130 mg, 0.236 mmol) in methanol (4 mL) was treated with 1 M lithium hydroxide solution at 60 °C for 3 h. The mixture was cooled to room temperature, concentrated in vacuo, and diluted with water. The solution was adjusted to pH ~4 and extracted three times with methylene chloride. The combined organic layers were dried (sodium sulfate) , filtered, and concentrated in vacuo. The residue was dissolved in a minimum of methylene chloride and hexane was added until the solution became cloudy. The mixture was concentrated slowly in vacuo to give 96 mg (76%) of the title compound. H NMR
(CDC13) δ 8.90 (s, 1H) , 8.23 (dd, J = 8, 1 Hz, 1H) , 7.41 (m,
2H) , 7.38 (s, 1H) , 7.29 (m, 2H) , 6.82 (d, J = 8 Hz, 1H) , 6.71 (d, J = 8 Hz, 1H) , 6.62 (d, J = 8 Hz, 1H) , 6.54 (s, 1H) , 4.25 (t, J = 6 Hz, 2H) , 4.22 (t, J = 6 Hz, 2H) , 2.59 (m, 4H) , 2.35 (quintet, J = 6 Hz, 2H) , 1.50 (hextet, J = 8
Hz, 2H) , 1.19 (t, J = 7 Hz, 3H) , 0.88 (t, J = 8 Hz, 3H) ;
+ TOF MS ES exact mass calculated for C30H32 O6S (p+1) : m/z
-1 = 534.1950. Found: 534.1957. IR (CHCI3, cm ) 2965, 1738,
1454.
Anal. Calcd for C30H31NO6S: C, 67.52; H, 5.86; N, 2.62.
Found: C, 67.19; H, 5.72; N, 2.53.
Example 4
Preparation of 2- (3-{3- [2-Ethyl-5-hydroxy-4- (2ff-pyrazol-3- yl)phenoxy]propoxy} -2-propyl-phenoxy)benzoic acid.
A. Preparation of 2- (3-{3- [5-benzyloxy-4- (3- dimethylaminoacryloyl) -2-ethyl-phenoxy]propoxy} -2- propylphenoxy)benzoic acid methyl ester.
A mixture of 2- (3- {3- [4-acetyl-5-benzyloxy-2- ethylphenoxy] propoxy} -2-propylphenoxy) benzoic acid methyl ester (3.07 g, 5.04 mmol) and dimethylformamide dimethylacetal (0.9 mL, 7 mmol) in N,N-dimethylformamide (3 mL) was heated at 110-120 °C for 35 h. The mixture was cooled to room temperature and diluted with a mixture of ethyl acetate and 1 N hydrochloric acid. The organic layer was separated, washed twice with water, once with saturated sodium chloride solution, dried (sodium sulfate), filtered, and concentrated in vacuo. Chromatography (silica gel, 30% ethyl acetate/70% hexane to ethyl acetate) of the residue provided 2.1 g (63%) of the title compound as a yellow oil.
+ TOF MS ES exact mass calculated for C4QH46N07 (p+1) : m/z
-1 = 652.3274. Found: 652.3270. IR (CHCI3, cm ) 2965, 1720,
1605.
Anal. Calcd for C4QH45N07: C, 73.71; H, 6.96; N, 2.15.
Found: C, 73.72; H, 6.95; N, 2.18.
B. Preparation of 2- (3-{3- [5-benzyloxy-2-ethyl-4- (2H- pyrazol-3-yl)phenoxy]propoxy} -2-propylphenoxy)benzoic acid.
A solution of 2- (3-{3- [5-benzyloxy-4- (3- dimethylaminoacryloyl) -2-ethyl-phenoxy] propoxy} -2- propylphenoxy) benzoic acid methyl ester (550 mg, 0.843 mmol in methanol (30 mL) was treated with 1 M lithium hydroxide solution at 60 °C for 3 h. The mixture was cooled to room temperature and diluted with ethyl acetate and 0.5 M hydrochloric acid. The organic layer was separated, washed with saturated sodium chloride solution, dried (sodium sulfate), filtered, and concentrated in vacuo. The residue was dissolved in methanol (15 mL) and treated with water (4 mL) and hydrazine monohydrate (0.50 mL, 7.7 mmol) at reflux for 3 h. The mixture was diluted with ethyl acetate and 1 N hydrochloric acid. The organic layer was separated, washed with saturated sodium chloride solution, dried (sodium sulfate), filtered and concentrated in vacuo. Chromatography (30% ethyl acetate/ 69% hexane/1% acetic acid) of the residue provided 350 mg (65%) of the title compound as the acetate salt. A portion of this material was free- based with sodium bicarbonate to provide an analytical
1 sample. H NMR (CDC13) δ 8.20 (dd, J = 8, 2 Hz, 1H) , 7.55
(s, 1H) , 7.44 (s, 1H) , 7.38 (m, 5H) , 7.15 (m, 2H) , 6.78 (d, J = 8 Hz, 1H) , 6.65 (d, J = 8 Hz, 1H) , 6.61 (d, J = 8 Hz,
1H) , 6.58 (s, 1H) , 6.55 (bs, 1H) , 5.18 (s, 2H) , 4.22 (t, J = 6 Hz, 2H) , 4.17 (t, J = 6 Hz, 2H) , 2.58 (m, 4H) , 2.30
(quintet, J = 6 Hz, 2H) , 1.47 (hextet, J = 8 Hz, 2H) , 1.18
+ (t, J = 7 Hz, 3H) , 0.88 (t, J = 8 Hz, 3H) ; TOF MS ES exact
mass calculated for C37H39N206 (p+1) : m/z = 607.2808.
-1 Found: 607.2831. IR (CHCI3, cm ) 2965, 1739, 1604, 1454.
Anal. Calcd for C37H38N206: C, 73.25; H, 6.31; N, 4.62. Found: C, 73.31; H, 6.30; N, 4.62.
C. Preparation of 2- (3-{3- [2-ethyl-5-hydroxy-4- (2ff-pyrazol- 3-yl)phenoxy]propoxy} -2-propylphenoxy)benzoic acid. A solution of 2- (3- {3- [5-benzyloxy-2-ethyl-4- (2H-pyrazol-3- yl) phenoxy] propoxy} -2-propylphenoxy) benzoic acid (300 mg, 0.490 mmol) in ethanethiol (2.5 mL) was treated with boron trifluoride etherate (2 mL) at room temperature for 3 h, at which time an additional portion of boron trifluoride etherate (1 mL) was added and stirring resumed for an additional 1 h. The mixture was diluted with diethyl ether and water. The organic layer was separated, washed with water, dried (sodium sulfate) , filtered, and concentrated in vacuo. Chromatography (silica gel, 15% ethyl acetate/85% hexane to 60% ethyl acetate/40% hexane) of the residue provided 60 mg (24%) of the title compound as a white solid.
1 H NMR (CDC13) δ 8.23 (d, J = 8 Hz, 1H) , 7.61 (s, 1H) , 7.42
(t, J = 7 Hz, 1H) , 7.30 (s, 1H) , 7.19 (d, J = 8 Hz, 1H) , 7.15 (d, J = 8 Hz, 1H) , 6.81 (d, J = 8 Hz, 1H) , 6.69 (d, J = 8 Hz, 1H) , 6.61 (s, 1H) , 6.60 (d, J = 8 Hz, 1H) , 6.54 (s, 1H) , 4.20 (m, 4H) , 2.58 (m, 4H) , 2.33 (quintet, J = 6 Hz, 2H) , 1 . 48 (hextet , J = 8 Hz , 2H) , 1 . 17 ( t , J = 8 Hz , 3H) ,
+ 0.86 (t, J = 7 Hz, 3H) ; TOF MS ES exact mass calculated
for C30H33N2O6 (p+1) : m/z = 517.2339. Found: 517.2334.
-1 IR (CHC13, cm ) 2965, 1738, 1454.
Anal. Calcd for C30H32N2O6: C, 69.75; H, 6.24; N, 5.42.
Found : C, 69.73; H, 6.33; N, 5.25
Example 5 Preparation of 2-{3- [3- (2-Ethyl-5-hydroxy-4-isoxazol-5-yl- phenoxy)propoxy] -2-propylphenoxy}benzoic acid.
A. Preparation of 2-{3- [3- (5-benzyloxy-2-ethyl-4-isoxazol- 5-yl-phenoxy)propoxy] -2-propylphenoxy}benzoic acid methyl ester.
A mixture of 2- (3- {3- [5-benzyloxy-4- (3- dimethylaminoacryloyl) -2-ethylphenoxy] propoxy} -2- propylphenoxy) benzoic acid methyl ester (280 mg, 0.43 mmol), hydroxylamine hydrochloride (75 mg, 1.1 mmol), and water (1 mL) in methanol (4 mL) was heated at reflux for 2 h. The mixture was cooled to room temperature and diluted with diethyl ether and water. The organic layer was separated, washed with saturated sodium chloride solution, dried (sodium sulfate), filtered, and concentrated in vacuo. Chromatography (silica gel, 10% ethyl acetate/90% hexane) of the residue provided 202 mg (76%) of the title compound as a
1 white solid. H NMR (CDC13) δ 8.20 (d, J = 2 Hz, 1H) , 7.88
(dd, J = 9, 2 Hz, 1H) , 7.79 (s, 1H) , 7.40 (m, 7H) , 7.08 (m, 2H) , 6.68 (d, J = 8 Hz, 1H) , 6.59 (s, 1H) , 6.58 (s, 1H) , 6.43 (d, J = 8 Hz, 1H) , 5.15 (s, 2H) , 4.21 (t, J = 6 Hz, 4H) , 3.82 (s, 3H) , 2.65 (m, 4H) , 2.33 (quintet, J = 6 Hz,
2H) , 1.56 (hextet, J = 8 Hz, 2H) , 1.20 (t, J = 7 Hz, 3H) ,
+ 0.90 (t, J = 7 Hz, 3H) ; TOF MS ES exact mass calculated
for C38H40NO7 (p+1) : m/z = 622.2805. Found: 622.2817 IR
-1 (CHCI3, cm ) 2964, 1720, 1461.
Anal. Calcd for C38H39 07: C, 73.41; H, 6.32; N, 2.25. Found: C, 73.20; H, 6.34; N, 2.27.
B. Preparation of 2- {3- [3- (2-ethyl-5-hydroxy-4-isoxazol-5- yl-phenoxy)propoxy] -2-propylphenoxy}benzoic acid methyl ester. A solution of 2- {3- [3- (5-benzyloxy-2-ethyl-4-isoxazol-5-yl- phenoxy) propoxy] -2-propylphenoxy}benzoic acid methyl ester (180 mg, 0.289 mmol) in ethanethiol (5 mL) was treated with boron trifluoride etherate (1.5 mL) at room temperature for 2 h, at which time an additional portion of boron trifluoride etherate (0.5 mL) was added and stirring resumed for an additional 1 h. The mixture was diluted with diethyl ether and water. The organic layer was separated, washed once with saturated sodium bicarbonate solution, once with saturated sodium chloride solution, dried (sodium sulfate) , filtered, and concentrated in vacuo. Chromatography (silica gel, 15% ethyl acetate/85% hexane) of the residue provided
1 94 mg (61%) of the title compound as a colorless oil. H
NMR (CDC13) δ 8.28 (d, J = 1 Hz, 1H) , 7.88 (dd, J = 8, 2 Hz,
1H) , 7.38 (t, J = 8 Hz,lH), 7.36 (s, 1H) , 7.08 (t, J = 8 Hz, 1H) , 7.05 (d, J = 8 Hz, 1H) , 6.81 (d, J = 8 Hz, 1H) , 6.67
(d, J = 8 Hz, 1H) , 6.50 (s, 1H) , 6.45 (s, 1H) , 6.43 (d, J = 8 Hz, 1H) , 4.20 (m, 4H) , 3.83 (s, 3H) , 2.62 (m, 4H) , 2.34
(quintet, J = 6 Hz, 2H) , 1.54 (hextet, J = 8 Hz, 2H) , 1.18
+ (t, J = 8 Hz, 3H) , 0.90 (t, J = 7 Hz, 3H) ; TOF MS ES exact
mass calculated for C31H34N07 (p+1) : m/z = 532.2335.
-1 Found: 532.2335. IR (CHCI3, cm ) 2964, 1715, 1601, 1461.
Anal. Calcd for C31H33 07: C, 70.04; H, 6.26; N, 2.63. Found: C, 70.13; H, 6.35; N, 2.63.
C. Preparation of 2-{3- [3- (2-ethyl-5-hydroxy-4-isoxazol-5- yl-phenoxy)propoxy] -2-propylphenoxy}benzoic acid.
To a solution of 2-{3- [3- (2-ethyl-5-hydroxy-4-isoxazol-5-yl- phenoxy) propoxy] -2-propylphenoxy}benzoic acid methyl ester (94 mg, 0.18 mmol) in methanol (3 mL) was added 1 M lithium hydroxide solution (1 mL) and the resulting mixture warmed at 60 °C for 3 h. The mixture was cooled to room temperature and concentrated in vacuo . The aqueous residue was diluted with water and the pH adjusted to ~4. The mixture was extracted three times with methylene chloride. The combined organic extracts were dried (sodium sulfate) , filtered, and concentrated in vacuo to provide 12 mg (13%) of the title compound as an off-white amorphous solid. H
NMR (CDC13) δ 8.26 (s, 1H) , 8.20 (dd, J = 8, 1 Hz, 1H) , 7.49
(t, J = 6 Hz, 1H) , 7.36 (s, 1H) , 7.18 (d, J = 8 Hz, 1H) , 7.15 (d, J = 8 Hz, 1H) , 7.02 (bs, 1H) , 6.80 (d, J = 8 Hz, 1H) , 6.69 (d, J = 8 Hz, 1H) , 6.60 (d, J = 8 Hz, 1H) , 6.50
(s, 1H) , 6.46 (s, 1H) , 4.22 (t, J = 6 Hz, 2H) , 4.19 (t, J = 6 Hz, 2H) ; 2.57 (m, 4H) , 2.34 (quintet, J = 6 Hz, 2H) , 1.47 (hextet , J = 8 Hz , 2H) , 1 . 16 ( t , J = 8 Hz , 3H) , 0 . 85 ( t , J =
+ 7 Hz, 3H) ; TOS MS ES exact mass calculated for C QH32N07
(p+1) : m/z = 518.2179. Found: 518.2175.
Anal. Calcd for C30H31NO7: C, 69.62; H, 6.04; N, 2.71. Found: C, 69.57; H, 6.15; N, 2.74.
Example 6 Preparation of 2- (3-{3- [2-Ethyl-5-hydroxy-4- OH[1,2,3] triazol-4-yl)phenoxy]propoxy} -2-propylphenoxy)benzoic acid.
A. Preparation of 2-{3- [3- (5-benzyloxy-4-bromo-2- ethylphenoxy)propoxy] -2-propylphenoxy} -benzoic acid methyl ester.
A mixture of 5-benzyloxy-4-bromo-l- (3-chloropropoxy) -2- ethylbenzene (1.19 g, 3.11 mmol), 2- (3-hydroxy-2- propylphenoxy) benzoic acid methyl ester (0.89 g, 3.1 mmol), potassium carbonate (1.29 g, 9.34 mmol), potassium iodide (0.52 g, 3.1 mmol), and methyl sulfoxide (2 mL) in 2- butanone (20 mL) was heated at reflux for 48 h. The mixture was cooled to room temperature, diluted with diethyl ether, and washed once with water. The organic layer was dried (sodium sulfate), filtered, and concentrated in vacuo. Chromatography (silica gel, 6% ethyl acetate/94% hexane) of the residue provided 1.34 g (68%) of the title compound as a
1 colorless oil. H NMR (CDCl3) δ 7.91 (dd, J = 8, 2 Hz, 1H) ,
7.50 (d, J = 7 Hz, 2H),.7.38 (m, 5H) , 7.15 (d, J = 8 Hz, 1H) , 7.10 (d, J = 8 Hz, 1H) , 6.83 (d, J = 8 Hz, 1H) , 6.71 (d, J = 8 Hz, 1H) , 6.55 (s, 1H) , 6.48 (, J = 8 Hz, 1H) , 5.16 (s, 2H) , 4.21 (t, J = 6 Hz, 2H) , 4.15 (t, J = 6 Hz, 2H) , 3.83 (s, 3H) , 2.68 (t, J = 8 Hz, 2H) , 2.58 (q, J = 7 Hz, 2H) , 2.31 (quintet, J = 6 Hz, 2H) , 1.58 (hextet, J = 6 Hz, 2H) , 1.17 (t, J = 7 Hz, 3H) , 0.93 (t, J = 7 Hz, 3H) .
B. Preparation of 2-{3- [3- (5-benzyloxy-2-ethyl-4- ethynylphenoxy)propoxy] -2-propyl-phenoxy}benzoic acid methyl ester.
A mixture of 2- {3- [3- (5-benzyloxy-4-bromo-2- ethylphenoxy) propoxy] -2-propylphenoxy} -benzoic acid methyl ester (1.50 g, 2.37 mmol), tri-n-butylethynyltin (0.82 mL, 2.8 mmol), and tetrakis (triphenylphosphine) palladium (0) (1.0 g, 0.95 mmol) in N,N-dimethylformamide (25 mL) was purged with argon and heated in a sealed tube at 120 °C for 24 h. The mixture was cooled to room temperature and filtered. The filtrate was diluted with ethyl acetate, washed four times with water, once with saturated sodium chloride solution, dried (sodium sulfate) , filtered, and concentrated in vacuo. Chromatography (silica gel, 10% ethyl acetate/90% hexane) of the residue provided 532 mg
1 (39%) of the title compound as a brown oil. H NMR (CDC13) δ 7.88 (dd, J = 8, 2 Hz, 1H) , 7.79 (s, 1H) , 7.20-7.50 (m, 6H) , 7.10 (d, J = 8 Hz, 1H) , 7.05 (d, J = 8 Hz, 1H) , 6.80 (d, J = 8 Hz, 1H) , 6.66 (d, J = 8 Hz, 1H) , 6.43 (m, 2H) , 5.16 (s, 2H) , 4.17 (t, J = 6 Hz, 2H) , 4.11 (t, J = 6 Hz, 2H) , 3.83 (s, 3H) , 3.23 (s, 1H) , 2.64 (t, J = 8 Hz, 2H) , 2.53 (q, J = 7 Hz, 2H) , 2.27 (quintet, J = 6 Hz, 2H) , 1.53
(m, 2H) , 1.13 (t, J = 7 Hz, 3H) , 0.89 (t, J = 7 Hz, 3H) ; TOF
+ MS ES exact mass calculated for C37H390g (p+1) : m/z = 579.2747. Found: 579.2739.
C. Preparation of 2- (3-{3- [5-benzyloxy-2-ethyl-4- (3H- [1,2,3] triazol-4-yl)phenoxy] -propoxy} -2- propylphenoxy)benzoic acid methyl ester.
A mixture of 2- {3- [3- (5-benzyloxy-2-ethyl-4- ethynylphenoxy) propoxy] -2-propyl-phenoxy}benzoic acid methyl ester (517 mg, 0.893 mmol) and trimethylsilyl azide (3.0 mL, 18 mmol) was heated in toluene (20 mL) in a sealed tube at 130 °C for 120 h. The mixture was cooled to room temperature and concentrated in vacuo . Chromatography (silica gel, 10% ethyl acetate/90% hexane to 50% ethyl acetate/50% hexane) of the residue provided 347 mg (88% based upon recovered starting material) of the title
1 compound as a brown solid. H NMR (CDC13) δ 8.10 (bs, 1H) ,
7.89 (dd, J = 8, 2 Hz, 1H) , 7.76 (s, 1H) , 7.40 (m, 7H) , 7.10 (d, J = 8 Hz, 1H) , 7.05 (d, J = 8 Hz, 1H) , 6.79 (d, J = 8 Hz, 1H) , 6.67 (d, J = 8 Hz, 1H) , 6.62 (s, 1H) , 6.43 (d, J = 8 Hz, 1H) , 5.18 (s, 2H) , 4.21 (m, 4H) , 3.82 (s, 3H) , 2.65 (m, 4H) , 2.32 (quintet, J = 6 Hz, 2H) , 1.56 (hextet, J = 8
Hz, 2H) , 1.21 (t, J = 8 Hz, 3H) , 0.90 (t, J = 7 Hz, 3H) ; TOF MS ES exact mass calculated for C37H4oN3°6 (p+1) : m/z =
-1 622.2917. Found: 622.2946. IR (CHC13, cm ) 3400, 1721,
1602, 1453.
Anal. Calcd for C37H39N306: C, 71.48; H, 6.32; N, 6.76.
Found: C, 70.28; H, 6.07; N, 6.54.
D. Preparation of 2- (3-{3- [2-ethyl-5-hydroxy-4- OH[1,2,3] triazol-4-yl)phenoxy] -propoxy} -2-propylphenoxy)benzoic acid methyl ester.
A solution of 2- (3-{3- [5-benzyloxy-2-ethyl-4-(3H- [1,2,3] triazol-4-yl) phenoxy] propoxy} -2-propylphenoxy) benzoic acid methyl ester (330 mg, 0.531 mmol) in ethanethiol (9 mL) was treated with boron trifluoride etherate (2.0 mL,16 mmol) for 1 h at room temperature and then with an additional portion of boron trifluoride etherate (1.0 mL) for 1 h. The mixture was diluted with diethyl ether and water. The organic layer was washed once with saturated sodium bicarbonate solution, once with saturated sodium chloride solution, dried (sodium sulfate), filtered, and concentrated in vacuo. Chromatography (silica gel, 30% ethyl acetate/70% hexane to 50% ethyl acetate/50% hexane) of the residue provided 180 mg (63%) of the title compound as a brown
1 solid. H NMR (CDCI3) δ 7.97 (s, 1H) , 7.88 (dd, J = 8, 2
Hz, 1H) , 7.37 (t, J = 8 Hz, 1H) , 7.31 (s, 1H) , 7.10 (d, J = 8 Hz, 1H) , 7.05 (d, J = 8 Hz, 1H) , 6.81 (d, J = 8 Hz, 1H) , 6.67 (d, J = 8 Hz, 1H) , 6.59 (s, 1H) , 6.43 (d, J = 8 Hz, 1H) , 4.20 (m, 4H) , 3.83 (s, 3H) , 2.63 (m, 4H) , 2.34 (quintet, J = 6 Hz, 2H) , 1.55 (hextet, J = 8 Hz, 2H) , 1.19
(t, J = 8 Hz, 3H) , 0.90 (t, J = 7 Hz, 3H) ; TOF MS ES exact
mass calculated for C30H34N3O6 (p+1) : m/z = 532.2447.
-1 Found: 532.2466. IR (CHCI3, cm ) 2964, 1718, 1453.
Anal. Calcd for C30H33N3O6: C, 67.78; H, 6.26; N, 7.90
Found: C, 66.80; H, 6.02; N, 7.53.
E. Preparation of 2- (3-{3- [2-ethyl-5-hydroxy-4- (3H- [1, 2, 3] triazol-4-yl)phenoxy] -propoxy}-2- propylphenoxy)benzoic acid.
A solution of 2-(3-{3- [2-ethyl-5-hydroxy-4- (3H- [1,2,3] triazol-4-yl) phenoxy] propoxy} -2-propylphenoxy) benzoic acid methyl ester (160 mg, 0.30 mmol) in methanol (5 mL) was treated 1 N lithium hydroxide solution (1.5 mL) at 60 °C for 3.5 h. The mixture was cooled to room temperature, diluted with water, and adjusted to ~pH 4. The resulting mixture was extracted three times with methylene chloride. The combined organic extracts were dried (sodium sulfate) , filtered, and concentrated in vacuo to provide 134 mg (86%)
1 of the title compound as a tan solid. H NMR (DMSO-d) δ 14.98 (bs, 1H) , 12.80 (bs, 1H) , 10.02 (bs, 1H) , 8.17 (bs, 1H) , 7.77 (dd, J = 7, 2 Hz, 1H) , 7.60 (bs, 1H) , 7.47 (t, J =
8 Hz, 1H) , 7.18 (t, J = 8 Hz, 1H) , 7.14 (t, J = 8 Hz, 1H) ,
6.82 (d, J = 8 Hz, 1H) , 6.68 (d, J = 8 Hz, 1H) , 6.57 (s,
1H) , 6.35 (d, J = 8 Hz, 1H) , 4.22 (t, J = 6 Hz, 2H) , 4.15
(t, J = 6 Hz, 2H) , 2.54 (m, 4H) , 2.25 (quintet, J = 6 Hz, 2H) , 1.45 (hextet, J = 8 Hz, 2H) , 1.11 (t, J = 7 Hz, 3H) ,
+ 0.81 (t, J = 7 Hz, 3H) ; TOF MS ES exact mass calculated
for C29H32N306 (p+1) : m/z = 518.2291. Found: 518.2302.
-1 IR (CHC13, cm ) 2965, 1738, 1454.
Anal. Calcd for C29H31N306: C, 67.30; H, 6.04; N, 8.12. Found: C, 67.15; H, 5.98; N, 7.93. Example 7
Preparation of 2-{3- [3- (2-Ethyl-5-hydroxy-4-pyrrol-l-yl- phenoxy)propoxy] -2-propyl-phenoxy}benzoic acid methyl ester.
A. Preparation of 5-benzyloxy-2-ethyl-4-pyrrol-l-yl-phenol.
To a mixture of potassium nitrosodisulfonate (40.0 g, 149 mmol) and potassium hydrogen phosphate (10 g) in water (1.2 L) at room temperature was added a solution of 4- ethylbenzene-1, 3-diol (10.0 g, 2.37 mmol) and potassium hydrogen phosphate (10.5 g) in water (150 mL) . The mixture was stirred for 15 min and adjusted to pH ~3. The solution was extracted three times with diethyl ether. The organic layer was dried (sodium sulfate) , filtered, and concentrated in vacuo. The residue was dissolved in acetonitrile (70 mL) and treated at room temperature with 65% 3-pyrroline (12 mL) . The resulting mixture was stirred for 1 h and concentrated in vacuo, dissolved in ethyl acetate and hexane, and filtered down a short column of silica gel. The resulting solution was concentrated in vacuo. The residue was dissolved in N,N-dimethylformamide (10 mL) and treated with benzyl bromide (0.85 mL, 7.1 mmol) and potassium carbonate (960 mg, 6.9 mmol) at room temperature for 15 h. The mixture was diluted with ethyl acetate, washed four times with water, once with saturated sodium chloride solution, dried (sodium sulfate) , filtered, and concentrated in vacuo. Chromatography (silica gel, ethyl acetate/hexane gradient) of the residue provided 316 mg (2%) of the title + compound. TOF MS ES exact mass calculated for C19H2QN02
(p+1) : m/z = 294.1494. Found: 294.1471.
B. Preparation of 1- [2-benzyloxy-4- (3-chloropropoxy) -5- ethylphenyl] -1H-pyrrole.
A mixture of 5-benzyloxy-2-ethyl-4-pyrrol-l-yl-phenol (316 mg, 1.08 mmol), potassium carbonate (223 mg, 1.62 mmol), and l-bromo-3-chloropropane (0.16 mL, 1.6 mmol) in N,N- dimethylformamide (5 mL) was stirred at room temperature for 18 h. The mixture was diluted with ethyl acetate and water, washed four times with water, once with saturated sodium chloride solution, dried (sodium sulfate) , filtered, and concentrated in vacuo. Chromatography (silica gel, 5% ethyl acetate/95% hexane) of the residue provided 314 mg (79%) of
+ the title compound as a colorless oil. TOF MS ES exact
mass calculated for C22H25NC102 (p+1): m/z = 370.1574. Found: 370.1548.
C. Preparation of 2-{3- [3- (5-benzyloxy-2-ethyl-4-pyrrol-l- yl-phenoxy)propoxy] -2-propylphenoxy}benzoic acid methyl ester.
A mixture of 1- [2-benzyloxy-4- (3-chloropropoxy) -5- ethylphenyl] -Iff-pyrrole (310 mg, 0.85 mmol) and sodium iodide (140 mg, 0.94 mol) in 2-butanone (5 mL) was heated at reflux for 6 h. The mixture was cooled to room temperature, filtered, and concentrated in vacuo. The residue was dissolved in N,N-dimethylformamide (7 mL) and treated with 2- (3-hydroxy-2-propylphenoxy) benzoic acid methyl ester (242 mg, 0.85 mmol) and potassium carbonate (129 g, 93 mmol) at room temperature for 15 h. The mixture was diluted with ethyl acetate and water, washed four times with water, once with saturated sodium chloride solution, dried (sodium sulfate), filtered, and concentrated in vacuo. Chromatography (silica gel, 5% ethyl acetate/95% hexane) of the residue provided 196 mg (37%) of the title compound as a
1 colorless oil. H NMR (CDCI3) δ 7.86 (dd, J = 8, 2 Hz, 1H) ,
7.37 (dt, J = 8, 2 Hz, 1H) , 7.30 (m, 5H) , 7.07 (m, 3H) , 6.84 (m, 2H) , 6.79 (d, J = 8 Hz, 1H) , 6.65 (d, J = 8 Hz, 1H) ,
6.58 (s, 1H) , 6.42 (d, J = 8 Hz, 1H) , 6.29 (m, 2H) , 4.92 (s,
2H) , 4.17 (t, J = 6 Hz, 2H) , 4.15 (t, J = 6 Hz, 2H) , 3.83
(s, 3H) , 2.65 (t, J = 8 Hz, 2H) , 2.58 (q, J = 7 Hz, 2H) ,
2.30 (quintet, J = 6 Hz , 2H) , 1.55 (hextet, J = 8 Hz, 2H) ,
+ 1.16 (t, J = 7 Hz, 3H) , 0.80 (t, J = 7 Hz, 3H) ; TOF MS ES
exact mass calculated for C39H42NOg (p+1): m/z = 620.3012. Found: 620.3021.
D. Preparation of 2-{3- [3- (2-ethyl-5-hydroxy-4-pyrrol-l-yl- phenoxy)propoxy] -2-propyl-phenoxy}benzoic acid methyl ester.
A solution of 2- {3- [3- (5-benzyloxy-2-ethyl-4-pyrrol-l-yl- phenoxy) propoxy] -2-propylphenoxy}benzoic acid methyl ester (195 mg, 0.315 mmol) in ethanethiol (5 mL) was treated with boron trifluoride etherate (1.3 mL, 9.5 mmol) at room temperature for 2.5 h. The mixture was diluted with diethyl ether and water. The organic layer was washed with saturated sodium bicarbonate solution, dried (sodium sulfate), filtered, and concentrated in vacuo. Chromatography (silica gel, 10% ethyl acetate/90% hexane) of the residue provided 39 mg (23%) of the title compound as a
1 colorless oil. H NMR (CDC13) δ 7.89 (d, J = 8 Hz , 1H) ,
7.37 (t, J = 8 Hz, 1H) , 7.07 (m, 2H) , 6.98 (s, 1H) , 6.68 (m, 3H) , 6.65 (d, J = 8 Hz, 1H) , 6.57 (s, 1H) , 6.42 (d, J = 8
Hz, 1H) , 6.35 (m, 2H) , 5.04 (bs, 1H) , 4.19 (m, 2H) , 3.83 (s, 3H) , 2.64 (t, J = 8 Hz, 2H) , 2.58 (q, J = 7 Hz, 2H) , 2.32 (quintet, J = 6 Hz, 2H) , 1.55 (m, 2H) , 1.14 (t, J = 7 Hz,
3H) , 0.90 (t, J = 7 Hz, 3H) ; TOF MS ES exact mass
calculated for C32H36 06 (p+1): m/z = 530.2543. Found: 530.2516.
Example 8
Preparation of 2- (3-{3- [4- (3-Bromo- [1, 2,4] thiadiazol-5-yl) ■ 2-ethyl-5-hydroxyphenoxy] -propoxy} -2-propylphenoxy)benzoic acid.
A. Preparation of 2- (3- {3- [5-benzyloxy-2-ethyl-4- (4,4,5, 5- tetramethyl- [1,3,2] dioxaborolan-2-yl)phenoxy]propoxy} -2- propylphenoxy)benzoic acid methyl ester.
A mixture of 2- {3- [3- (5-benzyloxy-4-bromo-2- ethylphenoxy) propoxy] -2-propylphenoxy} -benzoic acid methyl ester (8.30 g, 13.1 mmol), triethylamine (5.2 mL, 39 mmol), and PdCl2(dppf) (320 mg, 0.39 mmol) in de-oxygenated toluene
(80 mL) was treated with a 1 M solution of 4,4,5,5- tetramethyl- [1, 3 , 2] dioxaborolane in tetrahydrofuran (20 mL, 20 mmol) and heated at reflux for 6 h. The mixture was filtered down a short column of silica gel and the filtrate concentrated in vacuo. Chromatography (silica gel, 35% ethyl acetate/65% hexane) of the residue provided a dark oil that was subjected to further chromatography (silica gel, hexane to 30% ethyl acetate/70% hexane) to give 7.70 g (84%)
1 of the title compound. H NMR (CDC13) δ 7.86 (dd, J = 8, 2
Hz, 1H) , 7.60 (d, J = 8 Hz, 2H) , 7.47 (s, 1H) , 7.34 (m, 3H) , 7.24 (t, J = 8 Hz, 1H) , 7.09 (d, J = 9 Hz, 1H) , 7.04 (d, J = 9 Hz, 1H) , 6.79 (d, J = 9 Hz, 1H) , 6.66 (d, J = 9 Hz, 1H) , 6.47 (s, 1H) , 6.43 (d, J = 8 Hz, 1H) , 5.07 (s, 2H) , 4.18 (m, 4H) , 3.81 (s, 3H) , 2.64 (t, J = 8 Hz, 2H) , 2.56 (q, J = 7 Hz, 2H) , 2.30 (quintet, J = 6 Hz, 2H) , 1.53 (hextet, J = 8
Hz, 2H) , 1.34 (s, 12H),1.14 (t, J = 7 Hz, 3H) , 0.89 (t, J =
+ 7 Hz, 3H) ; TOF MS ES exact mass calculated for C41H53NB08
(p + NH4) : m/z = 698.3864. Found: 698.3889. IR (CHCI3,
-1 cm ) 2964, 1720, 1604, 1453.
Anal. Calcd for C41H49B08: C, 72.35; H, 7.26. Found: C, 72.30; H, 7.12.
B. Preparation of 2- (3-{3- [5-benzyloxy-4- (3-bromo- [1,2,4] thiadiazol-5-yl) -2-ethyl-phenoxy]propoxy} -2- propylphenoxy)benzoic acid methyl ester.
A mixture of 2- (3- {3- [5-benzyloxy-2-ethyl-4- (4, 4, 5 , 5- tetramethyl- [1,3,2] dioxaborolan-2-yl) phenoxy] propoxy} -2- propylphenoxy) benzoic acid methyl ester (310 mg, 0.46 mmol), 3-bromo-5-chloro-l, 2, 4-thiadiazole (120 mg, 0.60 mmol), cesium carbonate (300 mg, 0.92 mmol), and PdCl2(dppf) (20 mg, 0.024 mmol) in de-oxygenated toluene (10 mL) was heated at 100 °C for 15 h. The mixture was diluted with a solution of 35% ethyl acetate/65% hexane and filtered down a short column of silica gel. The filtrate was concentrated in vacuo. Chromatography (silica gel, hexane to 30% ethyl acetate/70% hexane) of the residue provided 232 mg (70%) of
1 the title compound. H NMR (CDCI3) δ 8.13 (s, 1H) , 7.87
(dd, J = 8, 2 Hz, 1H) , 7.44 (m, 2H) , 7.37 (m, 4H) , 7.08 (t, dJ = 8, 1 Hz, 1H) , 7.04 (d, J = 9 Hz, 1H) , 6.78 (d, J = 9 Hz, 1H) , 6.66 (d, J = 9 Hz, 1H) , 6.55 (s, 1H) , 6.43 (d, J = 8 Hz, 1H) , 5.28 (s, 2H) , 4.21 (t, J = 6 Hz, 2H) , 4.19 (t, J = 6 Hz, 2H) , 3.81 (s, 3H) , 2.62 (m, 4H) , 2.34 (quintet, J 6 Hz, 2H) , 1.55 (hextet, J = 8 Hz, 2H) , 1.17 (t, J = 7 Hz,
3H) , 0.88 (t, J = 7 Hz, 3H) ; MS ES m/e 717, 719.
C. Preparation of 2- (3-{3- [4- (3-bromo- [1, 2,4] thiadiazol-5- yl) -2-ethyl-5-hydroxyphenoxy]propoxy}-2- propylphenoxy)benzoic acid. A solution of 2- (3- {3- [5-benzyloxy-4- (3-bromo-
[1,2,4] thiadiazol-5-yl) -2-ethyl-phenoxy] propoxy} -2- propylphenoxy) benzoic acid methyl ester (230 mg, 0.31 mmol) in ethanethiol (4 mL) was treated with boron trifluoride etherate (0.32 mL, 2.5 mmol) at room temperature for 6 h, at which time an additional portion of boron trifluoride etherate was added and stirring continued for 7 h. The reaction mixture was diluted with water, concentrated in vacuo, and extracted with diethyl ether. The residue was dissolved in methanol (5 mL) and treated with 1 N lithium hydroxide solution (2 mL) at 65 °C for 1 h. The mixture was concentrated in vacuo and the residue diluted with water and adjusted to ~pH 3 with 1 N hydrochloric acid. The resulting precipitate was collected via vacuum filtration and dissolved in dilute aqueous base. Reverse phase chromatography (1:1 acetonitrile/water) provided 43 mg (23%)
1 of the title compound as a yellow solid. H NMR (DMSO-dg) δ
7.85 (s, 1H) , 7.80 (dd, J = 8, 2 Hz, 1H) , 7.45 (m, 2H) , 7.15 (m, 3H) , 6.83 (d, J = 9 Hz, 1H) , 6.80 (d, J = 9 Hz, 1H) , 6.62 (s, 1H) , 6.35 (d, J = 9 Hz, 1H) , 4.20 (m, 4H) , 2.55 (m, 4H) , 2.27 (quintet, J = 5 Hz, 2H) , 1.44 (hextet, J = 8 Hz, 2H) , 1.13 (t, J = 7 Hz, 3H) , 0.81 (t, J = 7 Hz, 3H) ; MS ES
+ -1 m/e 551 (p+NH4 -Br) ; IR (KBr, cm ) 2900, 1696, 1603, 1461.
Anal. Calcd for C29H29BrN206S : C, 56.77; H, 4.76; N, 4.56. Found: C, 56.63; H, 4.72; N, 3.98.
Example 9 Preparation of 2-{3- [3- ^-Ethyl-S-hydroxy^-thiophen^-yl- phenoxy^ropoxy] -2-propyl-phenoxy}benzoic acid sodium salt.
A. Preparation of 2-{3- [3- (2-ethyl-5-hydroxy-4-thiophen-2- yl-phenoxy)propoxy] -2-propylphenoxy}benzoic acid methyl ester.
A mixture of 2- (3- {3- [5-benzyloxy-2-ethyl-4- (4, 4, 5, 5- tetramethyl- [1,3,2] dioxaborolan-2-yl) phenoxy] propoxy} -2- propylphenoxy) benzoic acid methyl ester (300 mg, 0.44 mmol), 2-bromothiophene (110 mg, 0.66 mmol), cesium carbonate (300 mg, 2.17 mmol), and PdCl2(dppf) (20 mg, 0.024 mmol) in de- oxygenated toluene (10 mL) was heated at 105 °C for 66 h. The mixture was cooled to room temperature and concentrated in vacuo. The residue was dissolved in methylene chloride and filtered down a short column of silica gel. The filtrate was concentrated in vacuo. Chromatography (silica gel, 30% ethyl acetate/70% hexane) of the residue provided an oil that was dissolved in ethanethiol (4 mL) and treated with boron trifluoride etherate (0.44 mL, 3.4 mmol) at room temperature for 3 h. The mixture was diluted with water and extracted with diethyl ether. The organic layer was dried (sodium sulfate), filtered, and concentrated in vacuo. Chromatography (silica gel, hexane to 30% ethyl acetate/70% hexane) of the residue provided 120 mg (50%) of the title
1 compound as a yellow film. H NMR (CDCI3) δ 7.85 (dd, J =
8, 2 Hz, 1H) , 7.35 (t, J = 8 Hz, 1H) , 7.15 (d, J = 7 Hz, 1H) , 7.03-7.15 (m, 5H) , 6.80 (d, J = 9 Hz, 1H) , 6.66 (d, J = 9 Hz, 1H) , 6.51 (s, 1H) , 6.42 (d, J = 8 Hz, 1H) , 5.44 (bs, 1H) , 4.18 (m, 4H) , 3.82 (s, 3H) , 2.62 (t, J = 8 Hz, 2H) , 2.58 (q, J = 7 Hz, 2H) , 2.54 (quintet, J = 6 Hz, 2H) , 1.52 (hextet, J = 8 Hz, 2H) , 1.16 (t, J = 7 Hz, 3H) , 0.90 (t, J =
7 Hz, 3H) ; MS ES m/e 545 (p - 1)
B. Preparation of 2-{3- [3-(2-ethyl-5-hydroxy-4-thiophen-2- yl-phenoxy)propoxy] -2-propylphenoxy}benzoic acid sodium salt.
A solution of 2- {3- [3- (2-ethyl-5-hydroxy-4-thiophen-2-yl- phenoxy) propoxy] -2-propylphenoxy}benzoic acid methyl ester (120 mg, 0.22 mmol) in methanol (3 mL) was treated with 1 N lithium hydroxide solution (0.5 mL) at room temperature for 1 h and then with an additional portion of 1 N lithium hydroxide solution (0.75 mL) for 18 h. The mixture was heated at 50 °C then concentrated in vacuo. The residue was acidified with dilute hydrochloric acid and extracted with diethyl ether. The organic layer was washed once with water and concentrated in vacuo. The residue was diluted with 1 N sodium hydroxide solution (0.22 mL) , diethyl ether, and toluene. The mixture was concentrated in vacuo, dissolved in methylene chloride, and concentrated in vacuo to provide
120 mg (98%) of the title compound as a green film. H NMR
(DMS0-d6) δ 7.71 (d, J = 8 Hz, 1H) , 7.42 (m, 2H) , 7.31 (m,
2H) , 7.10 ( , 2H) , 6.99 (m, 1H) , 6.76 (t, J = 7 Hz, 2H) , 6.52 (s, 1H) , 6.30 (d, J = 8 Hz, 1H) , 4.16 (t, J = 7 Hz, 2H) , 4.07 (t, J = 7 Hz, 2H) , 2.50 (m, 4H) , 2.20 (m, 2H) , 1 . 40 (m, 2H) , 1 . 06 ( t , J = 8 Hz , 3H) , 0 . 77 ( t , J = 7 Hz ,
+ + -1
3H) ; MS ES m/e 533 (p + 1 - Na ) . IR (CHCI3, cm ) 2900,
1738, 1604, 1454.
Example 10
Preparation of 2- (3-{3- [2-Ethyl-5-hydroxy-4- (1-methyl-lH- pyrazol-4-yl) -phenoxy]propoxy}-2-propylphenoxy)benzoic acid.
A. Preparation of 4-iodo-l-methylpyrazole (Known compound: RN 39806-90-1).
To a solution of 4-iodopyrazole (1.3 g, 6.8 mmol) in dioxane (10 mL) was added iodomethane (0.42 mL, 6.8 mmol) and the resulting mixture stirred at room temperature for 96 h. The mixture was concentrated in vacuo and the residue mixed with methylene chloride and filtered. The filtrate was concentrated in vacuo to provide 1.35 g (95%) of the title
1 compound as a colorless oil. H NMR (CDCI3) δ 7.47 (s, 1H) ,
7.38 (s, 1H) , 3.90 (s, 3H)
B. Preparation of 2- (3-{3- [5-benzyloxy-2-ethyl-4- (1-methyl- lH-pyrazol-4-yl)phenoxy] -propoxy} -2-propylphenoxy)benzoic acid methyl ester.
A mixture of 2- (3- {3- [5-benzyloxy-2-ethyl-4- (4 , 4, 5 , 5- tetramethyl- [1,3,2] dioxaborolan-2-yl) phenoxy] propoxy} -2- propylphenoxy) benzoic acid methyl ester (1.00 g, 1.47 mmol), 4-iodo-l-methylpyrazole (450 mg, 2.16 mmol), cesium carbonate (1.20 g, 3.62 mmol), and PdCl2(dppf) (72 mg, 0.088 mmol) in de-oxygenated toluene (35 mL) was heated at 100 °C for 24 h. Additional portions of 4-iodo-l-methylpyrazole
(-30 mg) and PdCl2(dppf) (-30 mg) were added and heating continued at 100 °C for 40 h. The mixture was cooled to room temperature, concentrated in vacuo, diluted with methylene chloride, and filtered down a short plug of silica gel. The filtrate was concentrated in vacuo. Chromatography (silica gel, 35% ethyl acetate/65% hexane to
65% ethyl acetate/35% hexane) of the residue provided 710 mg
1 (76%) of the title compound. H NMR (CDCI3) δ 7.86 (dd, J =
8, 2 Hz, 1H) , 7.80 (s, 1H) , 7.69 (s, 1H) , 7.37 (m, 6H) , 7.28 (s, 1H) , 7.09 (d, J = 9 Hz, 1H) , 7.04 (d, J = 9 Hz, 1H) , 6.78 (d, J = 9 Hz, 1H) , 6.67 (d, J = 9 Hz, 1H) , 6.56 (s, 1H) , 6.42 (d, J = 8 Hz, 1H) , 5.08 (s, 2H) , 4.18 (t, J = 6 Hz, 2H) , 4.15 (t, J = 6 Hz, 2H) , 3.85 (s, 3H) , 3.81 (s, 3H) , 2.63 (t, J = 8 Hz, 2H) , 2.59 (q, J = 7 Hz, 2H) , 2.30 (quintet, J = 6 Hz, 2H) , 1.55 (hextet, J = 8 Hz, 2H) , 1.23 (t, J = 7 Hz, 3H) , 0.89 (t, J = 7 Hz, 3H) .
C. Preparation of 2- (3-{3- [2-ethyl-5-hydroxy-4- (1-methyl- lH-pyrazol-4-yl) -phenoxy]propoxy} -2-propylphenoxy)benzoic acid.
A solution of 2- (3-{3- [5-benzyloxy-2-ethyl-4- (1-methyl-lff- pyrazol-4-yl) phenoxy] propoxy} -2-propylphenoxy) benzoic acid methyl ester (710 mg, 1.12 mmol) in ethanethiol (5 mL) was treated with boron trifluoride etherate (1.42 mL, 11.2 mmol) at room temperature for 20 h. The reaction mixture was diluted with water, concentrated in vacuo, and extracted with diethyl ether. The organic layer was dried (magnesium sulfate), filtered, and concentrated in vacuo. The residue was triturated twice with hexane and the residue dissolved in methanol (5 mL) . This solution was treated with 1 N lithium hydroxide solution (5 mL) at -95 °C for 2 h. The mixture was concentrated in vacuo and the residue diluted with water, washed twice with diethyl ether, and the aqueous layer acidified with 1 N hydrochloric acid. The resulting solution was extracted with diethyl ether. The organic layer was dried (magnesium sulfate) , filtered, and concentrated in vacuo. Chromatography (silica gel, 10% methanol/90% methylene chloride) provided 338 mg (57%) of
1 the title compound as a tan foam. H NMR (DMS0-d6) δ 12.85
(bs, 1H) , 9.50 (bs, 1H) , 7.98 (s, 1H) , 7.78 (m, 2H) , 7.48 (dt, J = 8, 2 Hz, 1H) , 7.44 (s, 1H) , 7.18 (t, J = 8 Hz, 1H) , 7.13 (t, J = 9 Hz, 1H) , 6.79 (d, J = 9 Hz, 1H) , 6.77 (d, J =
9 Hz, 1H) , 6.53 (s, 1H) , 6.35 (d, J = 9 Hz, 1H) , 4.20 (t, J = 6 Hz, 2H) , 4.08 (t, J = 6 Hz, 2H) , 3.85 (s, 3H) , 2.50 (m,
4H) , 2.24 (quintet, J = 5 Hz, 2H) , 1.45 (hextet, J = 8 Hz,
+ 2H) , 1.09 (t, J = 7 Hz, 3H) , 0.82 (t, J = 7 Hz, 3H) ; MS ES
-1 m/e 531 (p+1); IR (KBr, cm ) 2961, 1697, 1602, 1460, 1222.
Anal. Calcd for C31H34N20g: C, 70.17; H, 6.46; N, 5.28. Found: C, 69.27; H, 6.08; N, 4.63.
Example 11
Preparation of 2- - [3- O-Ethyl-S-hydroxy^-thiazol^-yl- phenoxyJpropoxy] -2-propyl-phenoxy}benzoic acid.
A. Preparation of 2-{3- [3- (5-benzyloxy-2-ethyl-4-thiazol-2- yl-phenoxy)propoxy] -2-propylphenoxy} enzoic acid methyl ester.
A mixture of 2- (3- {3- [5-benzyloxy-2-ethyl-4- (4 , 4, 5 , 5- tetramethyl- [1,3,2] dioxaborolan-2-yl) phenoxy] propoxy} -2- propylphenoxy) benzoic acid methyl ester (960 mg, 1.41 mmol), 2-bromothiazole (0.25 mL, 2.8 mmol), cesium carbonate (1.15 g, 3.52 mmol), and PdCl2(dppf) (35 mg, 0.040 mmol) in de- oxygenated toluene (35 mL) was heated at 60 °C for 16 h then at 100 °C for 7 h. Additional portions of 2-bromothiazole
(0.13 mL) and PdCl2(dppf) (-30 mg) were added and heating continued at 100 °C for 72 h. The mixture was cooled to room temperature, concentrated in vacuo, diluted with methylene chloride, and filtered down a short plug of silica gel. The filtrate was concentrated in vacuo.
Chromatography (silica gel, hexane to 35% ethyl acetate/65% hexane) of the residue provided 282 mg (31%) of the title
1 compound. H NMR (CDCI3) δ 8.20 (s, 1H) , 7.86 (dd, J = 8, 1
Hz, 1H) , 7.82 (d, J = 3 Hz, 1H) , 7.49 (d, J = 7 Hz, 2H) , 7.35 (m, 4H) , 7.23 (d, J = 3 Hz, 1H) , 7.09 (d, J = 9 Hz,
1H) , 7.04 (d, J = 9 Hz, 1H) , 6.78 (d, J = 9 Hz, 1H) , 6.65
(d, J = 9 Hz, 1H) , 6.57 (s, 1H) , 6.42 (d, J = 8 Hz, 1H) ,
5.24 (s, 2H) , 4.17 (m, 4H) , 3.81 (s, 3H) , 2.63 (m, 4H) , 2.33
(quintet, J = 6 Hz, 2H) , 1.55 (hextet, J = 8 Hz, 2H) , 1.19 (t, J = 7 Hz, 3H) , 0.88 (t, J = 7 Hz, 3H) .
B. Preparation of 2-{3- [3- (2-ethyl-5-hydroxy-4-thiazol-2- yl-phenoxy)propoxy] -2-propylphenoxy}benzoic acid methyl ester.
A solution of 2-{3- [3- (5-benzyloxy-2-ethyl-4-thiazol-2-yl- phenoxy) propoxy] -2-propylphenoxy}benzoic acid methyl ester (282 mg, 0.442 mmol) in ethanethiol (3 mL) was treated with boron trifluoride etherate (0.56 mL, 4.4 mmol) at room temperature for 3 h. The reaction mixture was diluted with water, concentrated in vacuo, and extracted with diethyl ether. The organic layer was dried (magnesium sulfate), filtered, and concentrated in vacuo. Chromatography (silica gel, ethyl acetate/hexane) provided 107 mg (44%) of the
1 title compound. H NMR (CDCI3) δ 7.88 (dd, J = 8, 2 Hz,
1H) , 7.80 (d, J = 4 Hz, 1H) , 7.35 (dt, J = 8, 2 Hz, 1H) ,
7.28 (d, J = 4 Hz, 1H) , 7.24 (s, 1H) , 7.09 (dt, J = 9, 2 Hz,
1H) , 7.05 (t, J = 9 Hz, 1H) , 6.79 (d, J = 9 Hz, 1H) , 6.66
(d, J = 9 Hz, 1H) , 6.61 (s, 1H) , 6.42 (d, J = 9 Hz, 1H) , 4.24 (t, J = 6 Hz, 2H) , 4.18 (t, J = 6 Hz, 2H) , 3.81 (s,
3H) , 2.63 (t, J = 7 Hz, 2H) , 2.58 (q, J = 7 Hz, 2H) , 2.34 (quintet, J = 6 Hz, 2H) , 1.52 (hextet, J = 8 Hz, 2H) , 1.17
+ (t, J = 7 Hz, 3H) , 0.88 (t, J = 7 Hz, 3H) ; MS ES m/e 548
(P+1) •
C. Preparation of 2-{3- [3- (2-ethyl-5-hydroxy-4-thiazol-2- yl-phenoxy)propoxy] -2-propylphenoxy}benzoic acid.
2- {3- [3- (2-Ethyl-5-hydroxy-4-thiazol-2-yl-phenoxy) propoxy] - 2-propylphenoxy}benzoic acid methyl ester (107 mg, 0.196 mmol) was dissolved in a 1:1 solution of methanol/dioxane (3 mL) and treated with 1 N lithium hydroxide solution (1 mL) at 60 °C for 2 h. The mixture was concentrated in vacuo and the residue diluted with water, washed twice with diethyl ether, and the aqueous layer acidified with 1 N hydrochloric acid. The resulting solution was extracted twice with methylene chloride and the combined organic layers dried (magnesium sulfate), filtered, and concentrated in vacuo.
Trituration (hexane) of the residue provided 72 mg (69%) of
1 the title compound as a tan powder. H NMR (CDCI3) δ 8.22
(dd, J = 8, 2 Hz, 1H) , 7.70 (d, J = 4 Hz, 1H) , 7.41 (dt, J = 8, 2 Hz, 1H) , 7.35 (s, 1H) , 7.18 (m, 3H) , 6.82 (d, J = 9 Hz,
1H) , 6.69 (d, J = 9 Hz, 1H) , 6.62 (d, J = 9 Hz, 1H) , 6.55
(s, 1H) , 4.22 (t, J = 6 Hz, 2H) , 4.21 (t, J = 6 Hz, 2H) ,
2.57 (m, 4H) , 2.35 (quintet, J = 6 Hz, 2H) , 1.49 (hextet, J = 8 Hz, 2H) , 1.18 (t, J = 7 Hz, 3H) , 0.86 (t, J = 7 Hz, 3H) ;
+ -1
MS ES m/e 534 (p+1); IR (KBr, cm ) 2957, 1695, 1599, 1457.
Anal. Calcd for C3oH31N06S: C, 67.52; H, 5.86; N, 2.62. Found: C, 67.44; H, 5.95; N, 2.55.
Example 12
Preparation of 2- (3-{3- [4- (3, 5-Dimethylisoxazol-4-yl) -2- ethyl-5-hydroxyphenoxy]propoxy}-2-propylphenoxy)benzoic acid sodium salt.
A mixture of 2- (3- {3- [5-benzyloxy-2-ethyl-4- (4, 4, 5, 5- tetramethyl- [1,3,2] dioxaborolan-2-yl) phenoxy] propoxy} -2- propylphenoxy) benzoic acid methyl ester (305 mg, 0.448 mmol), 3 , 5-dimethyl-4-iodoisoxazole (110 mg, 0.493 mmol), cesium carbonate (293 mg, 0.899 mmol), and PdCl2(dppf) (15 mg, 0.018 mmol) in de-oxygenated toluene (10 mL) was heated at 95 °C for 10 h. Additional portions of 3 , 5-dimethyl-4- iodoisoxazole (110 mg) , cesium carbonate (260 mg) , and
PdCl2(dppf) (-15 mg) were added and heating continued at 110 °C for 20 h. The mixture was cooled to room temperature, concentrated in vacuo, diluted with methylene chloride, and filtered down a short plug of silica gel with 20% ethyl acetate/80% hexane. The filtrate was concentrated in vacuo. The resulting colorless oil was dissolved in methylene chloride (4 mL) , cooled to 0 °C, and treated with iodotrimethylsilane (0.40 mL, 2.7 mmol). The resulting mixture was allowed to warm to room temperature and stirred for 18 h. An additional portion of iodotrimethylsilane (0.70 mL) was added and stirring continued for 72 h. The mixture was poured into dilute sodium thiosulfate solution. The organic layer was separated, washed with water, dried (sodium sulfate), filtered, and concentrated in vacuo. The resulting foam was dissolved in a 1:1 mixture of tetrahydrofuran/1 N hydrochloric acid (5 mL) and stirred at room temperature for 18 h. The mixture was concentrated in vacuo and treated with 1 equivalent 1 N sodium hydroxide solution in ether. The resulting mixture was concentrated in vacuo to provide 59 mg (23%) of the title compound as an
1 off-white solid. H NMR (DMS0-d6) δ 7.40 (dd, J = 9, 2 Hz, 1H) , 7.13 (dt, J = 8, 2 Hz, 1H) , 6.97 (m, 2H) , 6.79 (s, 1H) , 6.68 (d, J = 9 Hz, 1H) , 6.65 (d, J = 9 Hz, 1H) , 6.60 (s, 1H) , 6.21 (d, J = 8 Hz, 1H) , 4.19 (t, J = 6 Hz, 2H) , 4.01 (t, J = 6 Hz, 2H) , 2.66 (t, J = 8 Hz, 2H) , 2.48 (q, J = 8 Hz, 2H) , 2.24 (s, 3H) , 2.17 (quintet, J = 6 Hz, 2H) , 2.07
(s, 3 H) , 1.49 (hextet, J = 8 Hz, 2H) , 1.07 (t, J = 7 Hz,
+ 3H) , 0.85 (t, J = 7 Hz, 3H) ; TOF MS ES exact mass
calculated for C32H36 07 (p+1): m/z = 546.2492. Found:
-1 546.2514; IR (KBr, cm ) 3400, 1605, 1460.
Example 13 Preparation of 2-{3- [3- (2-Ethyl-4-furan-2-yl-5- hydroxyphenoxy)propoxy] -2-propylphenoxy} -benzoic acid sodium salt.
A. Preparation of 2-{3- [3- (4-bromo-2-ethyl-5- hydroxyphenoxy)propoxy] -2-propylphenoxy}benzoic acid methyl ester.
A solution of 2-{3- [3- (5-benzyloxy-4-bromo-2- ethylphenoxy) propoxy] -2-propylphenoxy} -benzoic acid methyl ester (2.50 g, 3.95 mmol) in methylene chloride (40 mL) was cooled to -70 °C and treated with boron tribromide (0.25 mL, 2.6 mmol) . After 25 min the mixture was poured into cold water and the resulting mixture extracted with methylene chloride. The combined organic extracts were washed once with water, once with saturated sodium chloride solution, dried (sodium sulfate) , filtered, and concentrated in vacuo to provide 1.1 g (52%) of the title compound as a pale
1 yellow oil. H NMR (CDCI3) δ 7.89 (d, J = 9 Hz, 1H) , 7.38
(t, J = 8 Hz, 1H) , 7.18 (s 1H) , 7.12 (d, J = 9 Hz, 1H) , 7.08 (d, J = 2 Hz, 1H) , 6.81 (d, J = 9 Hz, 1H) , 6.68 (d, J = 9 Hz, 1H) , 6.56 (s, 1H) , 6.46 (d, J = 9 Hz, 1H) , 5.40 (s, 1H) , 4.18 (t, J = 6 Hz, 2H) , 4.11 (t, J = 6 Hz, 2H) , 3.84 (s, 3H) , 2.65 (t, J = 8 Hz, 2H) , 2.54 (q, J = 7 Hz, 2H) , 2.32 (quintet, J = 6 Hz, 2H) , 1.54 (hextet, J = 8 Hz, 2H) , 1.13
(t, J = 7 Hz, 3H) , 0.89 (t, J = 7 Hz, 3H) ; MS ES m/z = 541
(M - H) , 543 (M - H + 2) .
B. Preparation of 2- (3-{3- [4-bromo-5- (tert- butyldimethylsilanyloxy) -2-ethylphenoxy] -propoxy} -2- propylphenoxy)benzoic acid methyl ester.
A solution of 2-{3- [3- (4-bromo-2-ethyl-5- hydroxyphenoxy) propoxy] -2-propylphenoxy}benzoic acid methyl ester (1.00 g, 1.84 mmol) in methylene chloride (20 mL) was treated with imidazole (0.19 g, 2.8 mmol) and fcert- butyldimethylsilyl chloride (0.388 g, 2.57 mmol) at room temperature for 2 h. The mixture was poured into water and the organic layer separated, washed once with water, once with saturated sodium chloride solution, filtered through a short pad of silica gel, and concentrated in vacuo to provide 1.1 g (91%) of the title compound as a colorless
1 oil. H NMR (CDC13) δ 7.88 (d, J = 9 Hz, 1H) , 7.38 (t, J = 8 Hz, 1H) , 7.22 (s 1H) , 7.12 (d, J = 9 Hz, 1H) , 7.08 (d, J = 2 Hz, 1H) , 6.80 (d, J = 9 Hz, 1H) , 6.69 (d, J = 9 Hz, 1H) , 6.45 (d, J = 9 Hz, 1H) , 6.40 (s, 1H) , 4.20 (t, J = 6 Hz, 2H) , 4.11 (t, J = 6 Hz, 2H) , 3.83 (s, 3H) , 2.64 (t, J = 8 Hz, 2H) , 2.54 (q, J = 7 Hz, 2H) , 2.32 (quintet, J = 6 Hz, 2H) , 1.54 (hextet, J = 8 Hz, 2H) , 1.13 (t, J = 7 Hz, 3H) , 1.03 (s, 9H) , 0.89 (t, J = 7 Hz, 3H) , 0.23 (s, 6H) .
C. Preparation of 2-{3- [3- (2-ethyl-4-furan-2-yl-5- hydroxyphenoxy)propoxy] -2-propyl-phenoxy}benzoic acid methyl ester.
A mixture of 2- (3- {3- [4-bromo-5- ( fcert- butyldimethylsilanyloxy) -2-ethylphenoxy] propoxy} -2- propylphenoxy) benzoic acid methyl ester (1.05 g, 1.60 mmol), furan-2-boronic acid (0.358 g, 3.20 mmol), tetrakis (triphenylphosphine)palladium(O) (0.185 g, 0.160 mmol) , and 2 M aqueous sodium carbonate solution (8 mL) in tetrahydrofuran (20 mL) was heated at reflux for 18 h. The mixture was cooled to room temperature, diluted with water, and extracted with ethyl acetate. The organic layer was separated, washed once with water, once with saturated sodium chloride solution, dried (sodium sulfate) , filtered, and concentrated in vacuo. Chromatography (silica gel, 10% ethyl acetate/90% hexane) of the residue provided 0.8 g
(94%) of the title compound as a colorless oil H NMR
(CDC13) δ 7.90 (d, J = 9 Hz, 1H) , 7.48 (s, 1H) , 7.38 (t, J
8 Hz, 1H) , 7.21 (s 1H) , 7.13 (s, 1H) , 7.10 (d, J = 9 Hz, 1H) , 7.07 (d, J = 2 Hz, 1H) , 6.81 (d, J = 9 Hz, 1H) , 6.69 (d, J = 9 Hz, 1H) , 6.52 (m, 3H) , 6.44 (d, J = 9 Hz, 1H) , 4.20 (m, 4H) , 3.83 (s, 3H) , 2.67 (t, J = 8 Hz, 2H) , 2.59 (q, J = 7 Hz, 2H) , 2.32 (quintet, J = 6 Hz , 2H) , 1.55 (hextet, J = 8 Hz, 2H) , 1.18 (t, J = 7 Hz, 3H) , 0.91 (t, J = 7 Hz,
3H) ; MS ES m/z = 589 (p + AcO ) .
Anal. Calcd for C32H3407: C, 72.43; H, 6.46. Found: C, 72.21; H, 6.15.
D. Preparation of 2-{3- [3- (2-ethyl-4-furan-2-yl-5- hydroxyphenoxy)propoxy] -2-propylphenoxy}benzoic acid sodium salt.
2- (3- [3- (2-Ethyl-4-furan-2-yl-5-hydroxyphenoxy) propoxy] -2- propylphenoxy}benzoic acid methyl ester (250 mg, 0.47 mmol) was dissolved in tetrahydrofuran (4 mL) and treated with 1 N lithium hydroxide solution (2 mL) at 50 °C for 16 h. The mixture was concentrated in vacuo and the residue diluted with water and extracted twice with ethyl acetate. The combined organic extracts were washed once with water, once with saturated sodium chloride solution, dried (sodium sulfate) , filtered, and concentrated in vacuo. The residue was dissolved in ethyl acetate and shaken with 1 N hydrochloric acid. The organic layer was dried (sodium sulfate), filtered, and concentrated in vacuo. The residue was dissolved in diethyl ether and treated with 1 N aqueous sodium hydroxide solution (0.32 mL) . The mixture was concentrated in vacuo and azeotroped successively with diethyl ether, chloroform, and diethyl ether and dried to provide 168 mg (66%) of the title product as a cream solid.
1 H NMR (DMSO-d6) δ 7.56 (s, 1H) , 7.44 (d, J = 8 Hz, 1H) ,
7.35 (s, 1H) , 7.13 (m, 1H) , 6.97 (m, 2H) , 6.77 (d, J = 2 Hz, 1H) , 6.65 (m, 4H) , 6.48 (d, J = 2 Hz, 1H) , 6.24 (d, J = 9 Hz, 1H) , 4.15 (t, J = 6 Hz, 2H) , 3.96 (t, J = 6 Hz, 2H) , 2.66 (t, J = 8 Hz, 2H) , 2.42 (q, J = 7 Hz, 2H) , 2.13 (quintet, J = 6 Hz, 2H) , 1.48 (hextet, J = 8 Hz, 2H) , 1.09
(t, J = 7 Hz, 3H) , 0.84 (t, J = 7 Hz, 3H) ; TOF MS ES
exact mass calculated for C31H33θ7 (p+1): m/z = 517.2226.
-1 Found: 517.2230. IR (KBr, cm ) 3400, 2961, 1599, 1460.
Example 14 Preparation of 2-(3-{3- [2-Ethyl-5-hydroxy-4-furan-3- yl]phenoxy]propoxy}-2-propylphenoxy)benzoic acid.
A. Preparation of 2-{3- [3- (2-ethyl-4-furan-3-yl-5- hydroxyphenoxy)propoxy] -2-propyl-phenoxy}benzoic acid methyl ester.
A mixture of 2- (3- {3- [4-bromo-5- ( tert- butyldimethylsilanyloxy) -2-ethylphenoxy] propoxy} -2- propylphenoxy) benzoic acid methyl ester (2.10 g, 3.19 mmol), furan-3-boronic acid (0.722 g, 6.45 mmol), tetrakis (triphenylphosphine)palladium(O) (0.37 g, 0.32 mmol) , and 2 M aqueous sodium carbonate solution (16 mL) in tetrahydrofuran (30 mL) was heated at reflux for 48 h. The mixture was cooled to room temperature, diluted with water, and extracted with ethyl acetate. The organic layer was separated, washed once with water, once with saturated sodium chloride solution, dried (sodium sulfate) , filtered, and concentrated in vacuo. Chromatography (silica gel, 15% ethyl acetate/85% hexane) of the residue provided 0.29 g
+ (17%) of the title compound as a yellow oil. TOF MS ES exact mass calculated for C32H3507 (p+1): m/z = 531.2383 Found: 531.2396.
B. Preparation of 2-{3- [3- (2-ethyl-4-furan-3-yl-5- hydroxyphenoxy)propoxy] -2-propylphenoxy}benzoic acid sodium salt.
2- {3- [3- (2-Ethyl-4-furan-3-yl-5-hydroxyphenoxy)propoxy] -2- propylphenoxy}benzoic acid methyl ester (170 mg, 0.32 mmol) was dissolved in tetrahydrofuran (4 mL) and methanol (1 mL) and treated with 1 N lithium hydroxide solution (4 mL) at 50 °C for 2 h. The mixture was concentrated in vacuo and the residue acidified with hydrochloric acid and the resulting mixture extracted twice with ethyl acetate. The combined organic extracts were washed once with water, once with saturated sodium chloride solution, dried (sodium sulfate) , filtered, and concentrated in vacuo. Chromatography (silica gel, 2% methanol/98% chloroform) of the residue gave 45 mg of material that was again submitted to chromatography
(silica gel, 1% methanol/99% chloroform) to provide 25 mg (15%) of the title compound as an oil. TOF MS ES exact mass calculated for C31H33θ7 (p+1) : m/z 517.226. Found: 517.2230.
Example 15 Preparation of 2- (3-{3- [2-Ethyl-5-hydroxy-4- (tetrahydrofuran-3-yl)phenoxy]propoxy}-2- propylphenoxy)benzoic acid sodium salt hemihydrate.
A. Preparation of 2- {3- [3- (5-benzyloxy-2-ethyl-4-furan-3- yl-phenoxy)propoxy] -2-propylphenoxy}benzoic acid methyl ester.
A mixture of 2-{3- [3- (5-benzyloxy-4-bromo-2- ethylphenoxy) propoxy] -2-propylphenoxy} -benzoic acid methyl ester (3.00 g, 4.73 mmol), furan-3-boronic acid (1.06 g, 9.47 mmol), tetrakis ( triphenylphosphine)palladium(O) (0.54 g, 0.47 mmol), and 2 M aqueous sodium carbonate solution (20 mL) in tetrahydrofuran (40 mL) was heated at 100 °C for 48 h. The mixture was cooled to room temperature, diluted with water, and extracted with ethyl acetate. The organic layer was separated, washed once with water, once with saturated sodium chloride solution, dried (sodium sulfate) , filtered, and concentrated in vacuo. Chromatography (silica gel, 10% ethyl acetate/90% hexane) of the residue provided 1.9 g
1 (65%) of the title compound as a yellow oil. H NMR (CDCI3) δ 7.88 (dd, J = 8, 2 Hz, 1H) , 7.87 (s, 1H) , 7.40 (m, 7H) , 7.26 (s 1H) , 7.05 (m, 2H) , 6.80 (d, J = 9 Hz, 1H) , 6.76 (d, J = 2 Hz, 1H) , 6.67 (d, J = 9 Hz, 1H) , 6.60 (s, 1H) , 6.43 (d, J = 9 Hz, 1H) , 5.11 (s, 2H) , 4.18 (m, 4H) , 3.83 (s, 3H) , 2.66 (t, J = 8 Hz, 2H) , 2.62 (q, J = 7 Hz, 2H) , 2.30 (quintet, J = 6 Hz, 2H) , 1.57 (hextet, J = 8 Hz, 2H) , 1.20
(t, J = 7 Hz, 3H) , 0.92 (t, J = 7 Hz, 3H) ; MS ES m/z = 621
-1 (p + 1); IR (CHCI3, cm ) 3000, 1727, 1603, 1461.
B. Preparation of 2- (3-{3- [2-ethyl-5-hydroxy-4- (tetrahydrofuran-3-yl)phenoxy] -propoxy} -2- propylphenoxy)benzoic acid methyl ester.
A solution of 2-{3- [3- (5-benzyloxy-2-ethyl-4-furan-3-yl- phenoxy) propoxy] -2-propylphenoxy}benzoic acid methyl ester (1.8 g, 2.9 mmol) in ethyl acetate (40 mL) was treated with 10% palladium-on-carbon (0.39 g) and hydrogenated at 48 psi and 45 °C for 72 h. The mixture was cooled to room
TM temperature, filtered through Celite , and the filtrate concentrated in vacuo to provide 1.2 g (77%) of the title
1 compound as a colorless oil. H NMR (CDCI3) δ 7.88 (dd, J =
8, 2 Hz, IH) , 7.57 (dt, J = 8, 2 Hz, IH) , 7.09 (d, J = 9 Hz, IH) , 7.04 (d, J = 9 Hz, IH) , 6.81 (d, J = 9 Hz, IH) , 6.80
(s, IH) , 6.67 (d, J = 9 Hz, IH) , 6.44 (d, J = 9 Hz, IH) , 6.43 (s, IH) , 4.19 (m, 3H) , 4.10 (m, 2H) , 4.02 (dd, J = 12, 3 Hz, IH) , 3.88 (dd, J = 12, 8 Hz, IH) , 3.84 (s, 3H) , 3.73
(q, J = 9 Hz, IH) , 3.45 (m, IH) , 2.64 (t, J = 8 Hz, 2H) , 2.53 (q, J = 7 Hz, 2H) , 2.38 (m, IH) , 2.28 (quintet, J = 6 Hz, 2H) , 1.99 (m, IH) , 1.55 (hextet, J = 8 Hz, 2H) , 1.15 (t,
J = 7 Hz, 3H) , 0.90 (t, J = 7 Hz, 3H) ; MS ES m/z = 593 (p +
-1 CH3COO ); IR (CHCI3, cm ) 2963, 1719, 1589, 1461.
Anal. Calcd for C32H3807: C, 71.89; H, 7.16. Found: C, 71.41; H, 7.06.
C. Preparation of 2-(3-{3- [2-ethyl-5-hydroxy-4- (tetrahydrofuran-3-yl)phenoxy] -propoxy} -2- propylphenoxy)benzoic acid sodium salt hemihydrate.
A solution of 2- (3- {3- [2-ethyl-5-hydroxy-4- ( tetrahydrofuran- 3-yl) henoxy] propoxy} -2-propylphenoxy) benzoic acid methyl ester (0.92 g, 1.7 mmol) in tetrahydrofuran (10 mL) and methanol (5 mL) was treated with 1 M aqueous lithium hydroxide solution (10 mL) at 55 °C for 2 h. The mixture was allowed to cool to room temperature and stirred for an additional 18 h. The mixture was concentrated in vacuo and the remaining aqueous mixture was washed once with diethyl ether. The aqueous layer was acidified with concentrated hydrochloric acid and the resulting solution extracted with ethyl acetate. The ethyl acetate layer was washed once with water, once with saturated sodium chloride solution, dried (sodium sulfate), filtered, and concentrated in vacuo. The resulting colorless oil was dissolved in diethyl ether and treated with 1 N aqueous sodium hydroxide solution (1.72 mL) . The resulting biphasic mixture was diluted with chloroform and concentrated in vacuo. Diethyl ether was added and the mixture concentrated in vacuo. The resulting white foam was dried in vacuo at room temperature for 60 h to provide 0.78 g (84%) of the title compound: mp 67-71 °C.
1 H NMR (DMSO-d6) δ 7.62 (dd, J = 8, 2 Hz, IH) , 7.30 (dt, J = 8, 2 Hz, IH) , 7.05 (m, 2H) , 6.85 (s, IH) , 6.73 (d, J = 9 Hz,
IH) , 6.70 (d, J = 9 Hz, IH) , 6.53 (s, IH) , 6.34 (d, J = 9
Hz, IH) , 4.15 (t, J = 6 Hz, 2H) , 4.04 (t, J = 6 Hz, 2H) ,
3.95 (m, IH) , 3.88 (m, IH) , 3.75 (q, J = 9 Hz, IH) , 3.49 (m
2H) , 2.60 (t, J = 8 Hz, 2H) , 2.45 (q, J = 7 Hz, 2H) , 2.15 (m, 3H) , 1.90 (m, IH) , 1.48 (hextet, J = 8 Hz, 2H) , 1.06 (t, J = 7 Hz , 3H) , 0 . 83 ( t , J = 7 Hz , 3H) ; MS ES m/ z = 519 (p -
+ -1
Na ); IR (CHC13, cm ) 2964, 1783, 1604, 1461.
Anal. Calcd for C31H35Na07 . 0.5 H20: C, 67.50; H, 6.58. Found: C, 67.76; H, 6.68.
Example 16 Preparation of 2-{3- [3- (2-Ethyl-5-hydroxy-4-pyrrolidin-2-yl- phenoxy)propoxy] -2-propyl-phenoxy}benzoic acid hydrochloride hydrate.
A. Preparation of 2- (2-benzyloxy-5-ethyl-4-{3- [3- (2- methoxycarbonylphenoxy) -2- propylphenoxy]propoxy}phenyl)pyrrole-l-carboxylic acid tert- butyl ester.
A mixture of 2- {3- [3- (5-benzyloxy-4-bromo-2- ethylphenoxy) propoxy] -2-propylphenoxy} -benzoic acid methyl ester (3.00 g, 4.73 mmol), N-boc pyrrole-2-boronic acid (1.99 g, 9.43 mmol) , tetrakis (triphenylphosphine)palladium(O) (0.54 g, 0.47 mol) , and 2 M aqueous sodium carbonate solution (25 mL) in tetrahydrofuran (60 mL) was heated at reflux for 40 h. The mixture was cooled to room temperature, diluted with water, and extracted with ethyl acetate. The organic layer was separated, washed once with water, once with saturated sodium chloride solution, dried (sodium sulfate) , filtered, and concentrated in vacuo. Chromatography (silica gel, 10% ethyl acetate/90% hexane) of the residue provided 2.6 g
1 (76%) of the title compound as a solid. H NMR (CDCI3) δ 7.88 (dd, J = 8, 2 Hz, IH) , 7.15-7.40 (m, 7H) , 7.08 (m, 3H) , 6.82 (d, J = 9 Hz, IH) , 6.68 (d, J = 9 Hz, IH) , 6.52 (s, IH) , 6.44 (d, J = 9 Hz, IH) , 6.23 (t, J = 4 Hz, IH) , 6.12 (m, IH) , 4.95 (s, 2H) , 4.20 (t, J = 6 Hz, 2H) ; 4.15 (t, J = 6 Hz, 2H) , 3.84 (s, 3H) , 2.66 (t, J = 8 Hz, 2H) , 2.60 (q, J = 7 Hz, 2H) , 2.30 (quintet, J = 6 Hz, 2H) , 1.57 (hextet, J =
8 Hz, 2H) , 1.28 (s, 9H) , 1.18 (t, J = 7 Hz, 3H) , 0.93 (t, J
+ = 7 Hz, 3H) ; TOS MS ES exact mass calculated for
+ C44H53N2°8 (p + NH4 ) : m/z = 737.3802. Found: 737.3804;
-1 IR (CHCI3, cm ) 2964, 1730, 1461.
Anal. Calcd for C44H49NOg : C, 73.41; H, 6.86; N, 1.94. Found: C, 73.76; H, 6.76; N, 2.04.
B. Preparation of 2- (5-ethyl-2-hydroxy-4-{3- [3- (2- methoxycarbonylphenoxy) -2-propylphenoxy]propoxy}phenyl) - pyrrolidine-1-carboxylic acid tert-butyl ester.
A solution of 2- (2-benzyloxy-5-ethyl-4-{3- [3- (2- methoxycarbonylphenoxy) -2- propylphenoxy]propoxy}phenyl)pyrrole-l-carboxylic acid tert- butyl ester (0.98 g, 1.4 mmol) in ethyl acetate (40 mL) was treated with 10% palladium-on-carbon (0.98 g) and hydrogenated at 45 psi and 45 °C for 25 h, at room temperature for 20 h, then at 45 °C for 19 h. The mixture
TM was cooled to room temperature, filtered through Celite , and the filtrate concentrated in vacuo to provide 0.76 g (88%) of the title compound as a colorless oil H NMR
(CDC13) δ 7.87 (dd, J = 8, 2 Hz, IH) , 7.37 (dt, J = 8, 2 Hz,
IH) , 7.10 (d, J = 9 Hz, IH) , 7.04 (d, J = 9 Hz, IH) , 6.91 (s, IH) , 6.81 (d, J = 9 Hz, IH) , 6.67 (d, J = 9 Hz, IH) , 6.47 (s, IH) , 6.44 (d, J = 9 Hz, IH) , 5.09 (m, IH) , 4.18 (d, J = 6 Hz, 2H) , 4.14 (t, J = 6 Hz, 2H) , 3.84 (s, 3H) , 3.45 (m, 2H) , 2.64 (t, J = 8 Hz, 2H) , 2.54 (m, 3H) , 2.25 (m, 5H) , 2.06 ( , IH) , 1.54 (hextet, J = 8 Hz, 2H) , 1.43 (s, 9H) , 1.15 (t, J = 7 Hz, 3H) , 0.90 (t, J = 7 Hz, 3H) .
C. Preparation of 2- (4-{3- [3- (2-carboxyphenoxy) -2- propylphenoxy]propoxy}-5-ethyl-2-hydroxyphenyl)pyrrolidine-
1-carboxylic acid tert-butyl ester lithium salt hydrate.
A solution of 2- (5-ethyl-2-hydroxy-4-{3- [3-(2- methoxycarbonylphenoxy) -2- propylphenoxy] propoxy}phenyl) pyrrolidine-1-carboxylic acid tert-butyl ester (0.114 g, 0.18 mmol) in a 1:1 mixture of methanol/tetrahydrofuran (4 mL) was treated with solution of 1 M lithium hydroxide (4 mL) at room temperature for 18 h. The mixture was concentrated in vacuo and the residue dissolved in water. The resulting mixture was extracted with ethyl acetate. The organic extract was dried (sodium sulfate), filtered, and concentrated in vacuo. The residue was diluted with diethyl ether, concentrated in vacuo, and
+ dried to provide 90 mg (78%) of the title compound. MS ES + -1 m/z = 620 (p + 1 - Li ) ; IR (KBr, cm ) 2964, 1672, 1603,
1416.
Anal. Calcd for C36H44NOgLi . H20: C, 67.17; H, 7.20; N,
2.18. Found: C, 66.72; H, 6.99; N, 2.27.
D. Preparation of 2-{3- [3- (2-ethyl-5-hydroxy-4-pyrrolidin- 2-yl-phenoxy)propoxy] -2-propylphenoxy}benzoic acid hydrochloride hydrate.
Into a solution of 2- (4-{3- [3- (2-carboxyphenoxy) -2- propylphenoxy] propoxy} -5-ethyl-2-hydroxyphenyl)pyrrolidine- 1-carboxylic acid tert-butyl ester lithium salt hydrate (0.100 g, 0.16 mmol) in anhydrous diethyl ether (5 mL) was bubbled gaseous HCI. The resulting mixture was allowed to stir for 1 h. The mixture was concentrated in vacuo. Chromatography (SCX cation exchange resin, 1:1 tetrahydrofuran/methanol to dilute ammonia/methanol) of the residue provided a tan solid. This material was dissolved in ether and treated with gaseous HCI. This mixture was concentrated in vacuo to provide 48 mg (52%) of the title
1 . _. compound. H NMR (DMSO-d6) δ 12.80 (bs, IH) , 10.12 (s, IH) , 9.34 (bs, IH) , 8.36 (bs, IH) , 7.79 (dd, J = 9, 2 Hz, IH) , 7.47 (dt, J = 8, 2 Hz, IH) , 7.17 (t, J = 8 Hz, IH) , 7.12 (d, J = 9 Hz, IH) , 7.07 (s, IH) , 6.80 (d, J = 9 Hz, IH) , 6.78 (d, J = 9 Hz, IH) , 6.58 (s, IH) , 6.35 (d, J = 9 Hz, IH) , 4.56 ( , IH) , 4.20 (t, J = 6 Hz, 2H) ; 4.11 (t, J = 6 Hz, 2H) , 3.25 (m, 2H) , 2.50 (m, 5H) , 1.90-2.60 (m, 5H) , 1.44
(hextet, J = 8 Hz, 2H) , 1.08 (t, J = 7 Hz, 3H) , 0.82 (t, J =
+ 7 Hz, 3H) ; TOS MS ES exact mass calculated for C31H38N06
(p + 1): m/z = 520.2699. Found: 520.2672.
Example 17 Preparation of 2-{3- [3- (2-Ethyl-5-hydroxy-4-thiophβn-3-yl- phenoxy)propoxy] -2-propyl-phenoxy}benzoic acid hydrate.
Known compound :
Sawyer et al . , J". Med. Chem. 1995, 38, 4411.
A. Preparation of 3- [2-benzyloxy-4- (3-chloropropoxy) -5- ethylphenyl] thiophene. A mixture of 4- (benzyloxy) -5-bromo- 2- (3-chloropropoxy) ethylbenzene (1.90 g, 5.30 mmol), 3- thiopheneboronic acid (2.00 g, 15.9 mmol), tetrakis ( triphenylphosphine) palladium(O) (312 mg, 0.270 mmol) , 2 M aqueous sodium carbonate solution (4 mL) , and n- propanol (4 mL) in toluene (16 mL) was refluxed for 4 h. The mixture was cooled to room temperature, diluted with diethyl ether, washed once with water and once with saturated sodium chloride solution. The organic layer was dried (magnesium sulfate) , filtered, and concentrated in vacuo. Chromatography (silica gel, 5% ethyl acetate/95% hexane) of the residue provided 1.54 g (80%) of the title
1 product as a white solid: mp 65-67 °C. H NMR (CDCI3) δ
7.58 (d, J = 2.8 Hz, IH) , 7.49 (d, J = 5.2 Hz, IH) , 7.45- 7.30 (m, 7H) , 6.62 (s, IH) , 5.13 (s, 2H) , 4.14 (t, J = 5.8 Hz, 2H) , 3.81 (t, J = 6.3 Hz, 2H) , 2.66 (q, J = 7.5 Hz, 2H) ,
2.29 (quintet, J = 6.0 Hz, 2H) , 1.24 (t, J = 7.5 Hz, 3H) ; MS
-1 FD m/e 386 (p) ; IR (CHCI3, cm ) 2969, 1613, 1501, 1138.
Anal. Calcd for C22H2302C1S: C, 68.29; H, 5.99. Found: C, 68.53; H, 6.00.
Known compound:
Sawyer et al.,
J. Med. Chem. 1995, 38, 4411.
B. Preparation of 2- [2-propyl-3- [3- [5- (benzyloxy) -2-ethyl- 4- (thiophen-3-yl)phenoxy]propoxy]phenoxy]benzonitrile.
A mixture of 4- (benzyloxy) -2- (3-chloropropoxy) -5- (thiophen- 3-yl) ethylbenzene (1.25 g, 3.23 mmol), 3- (2-cyanophenoxy) -2- propyIphenol (0.82 g, 3.2 mmol), potassium iodide (0.21 g, 1.3 mmol), potassium carbonate (1.12 g, 8.08 mmol), and methyl sulfoxide (2 mL) in 2-butanone (10 mL) was refluxed for 60 h. The mixture was cooled to room temperature, diluted with ether, and washed with water. The organic layer was dried (magnesium sulfate) , filtered, and concentrated in vacuo. Chromatography (silica gel, 5% ethyl acetate/95% hexane) of the residue provided 1.31 g (67%) of
1 the title product as a colorless oil. H NMR (CDCI3) δ 7.66
(d, J = 7.8 Hz, IH) , 7.57 (d, J = 2.9 Hz, IH) , 7.48 (d, J = 5.2 Hz, IH) , 7.45-7.25 ( , 8H) , 7.20 (t, J = 8.2 Hz, IH) ,
7.10 (t, J = 8.1 Hz, IH) , 6.82 (d, J = 8.3 Hz, IH) , 6.77 (d, J = 8.6 Hz, IH) , 6.64 (s, IH) , 6.63 (d, J = 6.4 Hz, IH) ,
5.11 (s, 2H) , 4.26 (t, J = 6.0 Hz, 2H) , 4.22 (t, J = 6.0 Hz, 2H) , 2.65 (m, 4H) , 2.36 (quintet, J = 5.9 Hz, 2H) , 1.58 (hextet, J = 7.5 Hz, 2H) , 1.24 (t, J = 7.5 Hz, 3H) , 0.95 (t,
-1 J = 7.3 Hz, 3H) ; MS FD m/e 603 (p) ; IR (CHCI3, cm ) 2967,
2250, 1613, 1501. Anal. Calcd for C38H37N04S: C, 75.59; H, 6.18; N, 2.32. Found: C, 74.65; H, 6.21; N, 2.57.
C. Preparation of 2- [2-propyl-3- [3- [2-ethyl-5-hydroxy-4- (thiophen-3-yl)phenoxy]propoxy]phenoxy]benzonitrile.
To a solution of 2- [2-propyl-3- [3- [5- (benzyloxy) -2-ethyl-4- (thiophen-3-yl) phenoxy] propoxy] phenoxy] benzonitrile (900 mg, 1.49 mmol) in methylene chloride (25 mL) cooled to -78 °C was added 1 M boron tribromide solution in methylene chloride (2.99 mL, 2.99 mmol) over 2 min. The resulting deep violet solution was stirred for 30 min and allowed to warm to room temperature. The mixture was diluted with water and shaken. The organic layer was separated, dried (magnesium sulfate), filtered, and concentrated in vacuo. Chromatography (silica gel, 25% ethyl acetate, 75% hexane) provided 400 mg (52%) of the title product as a colorless
1 oil. H NMR (CDC13) δ 7.84 (d, J = 4.8 Hz, IH) , 7.71 (d, J =
4.9 Hz, IH) , 7.66 (d, J = 7.7 Hz, IH) , 7.62 (s, IH) , 7.42 (t, J = 7.1 Hz, IH) , 7.27 (t, J = 6.6 Hz, IH) , 7.20 (s, IH) , 7.08 (t, J = 6.9 Hz, IH) , 6.85 (s, IH) , 6.89 (d, J = 8.1 Hz, IH) , 6.74 (d, J = 8.5 Hz, IH) , 6.60 (d, J = 7.6 Hz, IH) , 4.71 (s, IH, -OH), 4.26 (t, J = 6.0 Hz, 4H) , 2.72 (q, J = 7.4 dHz, 2H) , 2.59 (t, J = 7.3 Hz, 2H) , 2.39 (quintet, J = 6.1 Hz, 2H) , 1.54 (hextet, J = 7.7 Hz, 2H) , 1.25 (t, J = 7.5 Hz, 3H) , 0.91 (t, J = 7.4 Hz, 3H) .
D. Preparation of 2- [2-propyl-3- [3- [2-ethyl-5-hydroxy-4- (thiophen-3-yl)phenoxy]propoxy]phenoxy]benzoic acid hydrate.
A solution of 2- [2-propyl-3- [3- [2-ethyl-5-hydroxy-4- (thiophen-3-yl) phenoxy] propoxy] phenoxy] benzonitrile (400 mg, 0.780 mmol) in 2 : 1 methanol/water (6 mL) was treated with
12.5 M aqueous sodium hydroxide (4.0 mL) at reflux for 36 h. The mixture was cooled to room temperature, diluted with water, and extracted once with diethyl ether. The aqueous layer was acidified with concentrated hydrochloric acid and extracted twice with methylene chloride. The combined methylene chloride layers were dried (magnesium sulfate) , filtered, and concentrated in vacuo to provide a tan solid:
1 mp 90-95 °C (dec). H NMR (CDC13) δ 8.24 (d, J = 7.8 Hz,
IH) , 7.47 (d, J = 5.0 Hz, IH) , 7.44 (t, J = 8.6 Hz, IH) , 7.36 (d, J = 3 Hz, IH) , 7.24 (d, J = 4.9 Hz, IH) , 7.19 (m, 2H) , 7.09 (s, IH) , 6.84 (d, J = 8.0 Hz, IH) , 6.73 (d, J = 8.3 Hz, IH) , 6.64 (d, J = 8.0 Hz, IH) , 6.55 (s, IH) , 5.38 (bs, IH, -OH), 4.26 (t, J = 6.2 Hz, 2H) , 4.21 (t, J = 7.1 Hz, 2H) , 2.60 (m, 4H) , 2.36 (quintet, J = 5.8 Hz, 2H) , 1.51
(hextet, J = 7.1 Hz, 2H) , 1.19 (t, J = 7.5 Hz, 3H) , 0.90 (t,
-1 J = 7.4 Hz, 3H) ; MS FD m/e 532 (p) ; IR (KBr, cm ) 3200
(br) , 2961, 1697, 1457, 1110. Anal. Calcd for C31H3206S .
H20: C, 67.62; H, 6.22. Found: C, 67.34; H, 5.87.
In one embodiment the compositions of the present invention are a combination of therapeutically effective amounts of the leukotriene (LTB4) antagonists, noted above, and a therapeutically effective amount of an anti-cancer agent or anti-cancer agents. The composition may be formulated with common excipients, diluents or carriers, and compressed into tablets, or formulated elixirs or solutions for convenient oral administration or administered by intramuscular intravenous routes. The compounds can be administered transdermally and maybe formulated as sustained relief dosage forms and the like.
In another embodiment, the anti-cancer agents are formulated independently of the leukotriene (LTB4) antagonists and are administered separately. The anti- cancer agents may be formulated with common excipients, diluents or carriers and administered by intravenous infusion. On the other hand, the anti-cancer agents may be formulated into liquids suitable for oral administration. Anti-cancer agents may also be compressed into tablets and administered orally. If the anti-cancer agents and the leukotrienes are administered separately, the anti-cancer agents may be administered before, after or during the administration of the leukotriene (LTB4) antagonists. If the anti-cancer agents are administered separately from the leukotrienes (LTB4) antagonists, they must be administered within a therapeutically effective interval.
The method of treating a human patient according to the present invention includes both the administration of the combination of leukotriene (LTB4) antagonists and an anti- cancer agent as well as the separate administration of the leukotriene (LTB4) antagonists and the anti-cancer agent. When administered separately, the leukotriene (LTB4) antagonists are formulated into formulations which may be administered by the oral and rectal routes, topically, parenterally, e.g., by injection and by continuous or discontinuous intra-arterial infusion, in the form of, for example, tablets, lozenges, sublingual tablets, sachets, cachets, elixirs, gels, suspensions, aerosols, ointments, for example, containing from 1 to 10% by weight of the active compound in a suitable base, soft and hard gelatin capsules, suppositories, injectable solutions and suspensions in physiologically acceptable media, and sterile packaged powders adsorbed onto a support material for making injectable solutions. Advantageously for this purpose, compositions may be provided in dosage unit form, preferably each dosage unit containing from about 5 to about 500 mg (from about 5 to 50 mg in the case of parenteral or inhalation administration, and from about 25 to 500 mg in the case of oral or rectal administration) of a compound of Formula I or Formula II. Dosages from about 0.5 to about 300 mg/kg per day, preferably 0.5 to 20 mg/kg, of active ingredient may be administered although it will, of course, readily be understood that the amount of the compound or compounds of Formula I actually to be administered will be determined by a physician, in the light of all the relevant circumstances including the condition to be treated, the choice of compound to be administered and the choice of route of administration and therefore the above preferred dosage range is not intended to limit the scope of the present invention in any way.
The formulations useful for separate administration of the leukotriene (LTB4) antagonists will normally consist of at least one compound selected from the compounds of Formula I and Formula II mixed with a carrier, or diluted by a carrier, or enclosed or encapsulated by an ingestible carrier in the form of a capsule, sachet, cachet, paper or other container or by a disposable container such as an ampoule. A carrier or diluent may be a solid, semi-solid or liquid material which serves as a vehicle, excipient or medium for the active therapeutic substance. Some examples of the diluents or carrier which may be employed in the pharmaceutical compositions of the present invention are lactose, dextrose, sucrose, sorbitol, mannitol, propylene glycol, liquid paraffin, white soft paraffin, kaolin, fumed silicon dioxide, microcrystalline cellulose, calcium silicate, silica, polyvinylpyrrolidone, cetostearyl alcohol, starch, modified starches, gum acacia, calcium phosphate, cocoa butter, ethoxylated esters, oil of theobroma, arachis oil, alginates, tragacanth, gelatin, syrup, methyl cellulose, polyoxyethylene sorbitan monolaurate, ethyl lactate, methyl and propyl hydroxybenzoate, sorbitan trioleate, sorbitan sesquioleate and oleyl alcohol and propellants such as trichloromonofluoromethane, dichlorodifluoromethane and dichlorotetrafluoroethane . In the case of tablets, a lubricant may be incorporated to prevent sticking and binding of the powdered ingredients in the dies and on the punch of the tableting machine. For such purpose there may be employed for instance aluminum, magnesium or calcium stearates, talc or mineral oil.
Preferred pharmaceutical forms of the present invention are capsules, tablets, suppositories, injectable solutions, creams and ointments. Especially preferred are formulations for inhalation application, such as an aerosol, and for oral ingestion.
Pharmaceutical Compositions of the Invention
The pharmaceutical composition of the invention comprises as essential ingredients: (a) an LTB4 antagonist, and
(b) an anti-cancer agent. When the pharmaceutical composition of the invention is prepared in injectable form it is a composition comprising as ingredients: (a) an LTB4 antagonist,
(b) an anti-cancer agent, and
(c) an injectable liquid carrier. Pharmaceutically acceptable carriers are those well known in the medical arts, such as sterile water, sterile water containing saline, and sterile water containing sugars and/or saline.
a. Ratio and Amount of Ingredients in the Composition of the Invention
The essential ingredients (a) an LTB4 antagonist and (b) anti-cancer compound are present in the formulation in such proportion that a dose of the formulation provides a pharmaceutically effective amount of each ingredient to the patient being treated. Typically, the weight ratio of LTB4 antagonist to anti-cancer agent 1:100 to 100 to 1, preferable from 10:1 to 1:10 and most preferable from 1:4 to 4:1.
The following formulation examples may employ as active compounds any of the leukotriene (LTB4) antagonists noted above. The examples are illustrative only and are not intended to limit the scope of the invention in any way.
FORMULATION EXAMPLE 1
Hard gelatin capsules are prepared using the following ingredients :
Quantity
(mg/capsule) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- ( 4-carboxy- phenoxy) phenyl) propanoic acid 250
Starch 200
Magnesium stearate 10
The above ingredients are mixed and filled into hard gelatin capsules in 460 mg quantities. FORMULATION EXAMPLE 2
A tablet is prepared using the ingredients below: Quantity
(mg/capsule) 1- (4- (Carboxymethoxy) phenyl) -1- (1H- tetrazol-5-yl) -6- (2-ethyl-4- (4- fluorophenyl) -5-hydroxyphenoxy) hexane 250
Cellulose, microcrystalline 400
Silicon dioxide, fumed 10
Magnesium stearate 5
The components are blended and compressed to form tablets each weighing 665 mg.
FORMULATION EXAMPLE 3
An aerosol solution is prepared containing the following components:
Weight % 3- [4- [7-Carboxy-9-oxo-3- [3- [2-ethyl-4-
(4-fluorophenyl) -5-hydroxyphenoxy] propoxy] - 9H-xanthene] ] propanoic acid 0.25
Ethanol 30.00
Propellant 11 10.25 (trichlorofluoromethane)
Propellant 12 29.75
(Dichlorodifluoromethane)
Propellant 114 29.75
(Dichlorotetrafluoroethane)
The active compound is dissolved in the ethanol and the solution is added to the propellant 11, cooled to -30°C. and transferred to a filling device. The required amount is then fed to a container and further filled with the pre-mixed propellants 12 and 114 by means of the cold-filled method or pressure-filled method. The valve units are then fitted to the container. FORMULATION EXAMPLE 4
Tablets each containing 60 mg of active ingredient are made up as follows :
2- [2-Propyl-3- [3- [2-ethyl-5-hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] phenoxy] - benzoic acid sodium salt 60 mg
Starch 45 mg
Microcrystalline cellulose 35 mg
Polyvinylpyrrolidone 4 mg (as 10% solution in water)
Sodium carboxymethyl starch 4.5 mg
Magnesium stearate 0.5 mg
Talc 1 mg
Total 150 mg
The active ingredient, starch and cellulose are passed through a No . 45 mesh (355 μm) U.S. sieve and mixed thoroughly. The solution of polyvinylpyrrolidone is mixed with the resultant powders which are then passed through a No. 14 mesh (1.4 mm) U.S. sieve. The granules so produced are dried at 50-60°C and passed through a No . 18 mesh (1.00 μm) U.S. sieve. The sodium carboxymethyl starch, magnesium stearate and talc, previously passed through a No . 60 mesh _ (250 μm) U.S. sieve, are then added to the granules which, after mixing, are compressed on a tablet machine to yield tablets each weighing 150 mg. FORMULATION EXAMPLE 5
Capsules each containing 80 mg of medicament are made as follows:
5- [3- [2- (1-Carboxy) ethyl] -4- [3- [2-ethyl-4- (4- fluorophenyl) -5-hydroxyphenoxy] propoxy] - phenyl] -4-pentynoic acid 80 mg
Starch 59 mg
Microcrystalline cellulose 59 mg
Magnesium stearate 2 mg
Total 200 mg
The active ingredient, cellulose, starch and magnesium stearate are blended, passed through a No . 45 mesh (355 μm) U.S. sieve, and filled into hard gelatin capsules in 200 mg quantities.
FORMULATION EXAMPLE 6
Suppositories each containing 225 mg of active ingredient are made as follows:
3- (5- (6- (4- (4-Fluorophenyl) -5-hydroxy-2- ethylphenoxy) propoxy) -2-carboxymethyl-
1,2,3, 4-tetrahydronaphthalen-l (2H) - one) propanoic acid 225 mg
Unsaturated or saturated fatty acid glycerides to 2,000 mg The active ingredient is passed through a No . 60 mesh (250 μm) U.S. sieve and suspended in the fatty acid glycerides previously melted using the minimum heat necessary. The mixture is then poured into a suppository mold of nominal 2 g capacity and allowed to cool.
FORMULATION EXAMPLE 7
Suspensions each containing 50 mg of medicament per 5 mL dose are made as follows:
2- [2-Propyl-3- [3- [2-ethyl-4- (4-fluorophenyl) - 5-hydroxyphenoxy] propoxy] phenoxy] benzoic acid 50 mg
Sodium carboxymethyl cellulose 50 mg Sugar 1 g
Methyl paraben 0.05 mg
Propyl paraben 0.03 mg
Flavor q.v.
Color q.v. Purified water to 5 mL
The medicament is passed through a No. 45 mesh (355 μm) U.S. sieve and mixed with the sodium carboxymethylcellulose, sugar, and a portion of the water to form a suspension. The parabens, flavor and color are dissolved and diluted with some of the water and added, with stirring. Sufficient water is then added to produce the required volume.
The leukotriene (LTB4) antagonists are generally administered prior, during and after the anti-cancer agent or agents are administered. If the leukotriene (LTB4) antagonists are administered before or after the anti-cancer agent or agents, they should be administered within a therapeutically effective interval.
ASSAY EXAMPLE 1
The Nude Mouse Xenograft test used to evaluate anti- oncolytic agents of this invention is well known and generally described in the textbook; Beverly A Teicher, Editor, Anticancer Drug Development Guide, Humana Press, Totowa, New Jersey, 1997, p.75-124 (ISBN 0-89603-461-5); the disclosure of which is incorporated herein by reference. The xenograft test is more particularly described as follows:
Male or female nude mice, selected as appropriate to the gender of the tumor (Charles River) , were treated with total body gamma Radiation (450 rads) . After 24 hours, human DU-145 prostate carcinoma, human H460 and Calu-6 non- small cell lung carcinomas, human HCT116 and HT29 colon carcinomas, and human MX-1 breast carcinoma (human DU-145 prostate carcinoma, human NCI-H460 and Calu-6 non-small cell lung carcinomas, and human HCT116 and HT29 colon carcinomas available from the American Type Culture Collection,
Manassas, VA; human MX-1 breast carcinoma available from the National Cancer Institute, Bethesda, MD) , prepared from a brie of donor tumors (5 x 106 cells) , were implanted subcutaneously in a hind-leg of the mice. The mice were treated with 2- [2-propyl-3- [3- [2-ethyl-5-hydroxy-4- (4- fluorophenyl) phenoxy] propoxy] phenoxy] benzoic acid (Formula IV) , at dosages of 30, 100, 200, or 300 mg per kilogram daily, administered orally, beginning 4 days after the tumor cell implantation. An anti-cancer agent (irinotecan, paclitaxel, 5-fluorouracil, carboplatin, mitoxantrone, oxaliplatin, or indomethacin) was administered intraperitoneally or intravenously (paclitaxel) at dosages ranging from 30, 30, 24, 50, 1.6, 5, and 5 mg/kg, respectively .
Tumor response was monitored by tumor volume measurements performed twice per week over the course of 60- 90 days. Body weights were determined as a general measurement of toxicity. The mice were divided into an untreated control group and multiple treatment groups with five mice in each group.
The data was analyzed by determining the mean tumor volume for the control group and each treatment group over the course of the experiment. The tumor growth delay was calculated as the difference in days for the treatment versus the control tumors to reach the volume of 1000 mm3.
Table 1
Mouse Xenograft Test Results Growth Delay of Colon Tumor11' With Oxaliplatin
(1) = Human HT29 colon carcinoma
Formula IV = the LTB4 antagonist, 2- [2-propyl-3- [3- [3- ethyl-5-hydroxy-4- (4- fluorophenyl) phenoxy] propoxy] phenoxy] benzoic acid OXAL = Oxaliplatin; (SP-4-2 ) - [ (IR, 2R) -1, 2- cyclohexanediamine-κN,KN' ] [ethanedioato (2-) -κ01,κ02] -
Platinum; CsHι4N20Pt; Chemical Abstract Registry Number
61825-94-3
Dose = milligrams per kilogram mouse body weight
TGD = average tumor growth delay in days sem = standard error of the mean Table 2
Mouse Xenograft Test Results Growth Delay of Colon Tumor'2' With Indomethacin
(2) = Human HT29 colon carcinoma
INDO = Indomethacin; 1- (4-Chlorobenzoyl) -5-methoxy-2- methyl-lH-indole-3-acetic acid; Cι9Hι6ClN0 ; molecular weight 357.81; Chemical Abstract Registry Number 53-86-1
Table 3
Mouse Xenograft Test Results Growth Delay of Colon Tumor13' With 5-Fluorouracil
(3) = Human HCT116 colon carcinoma 5-FU = 5-Fluorouracil; 5-Fluoro-2 , 4 (IH, 3H) - pyrimidinedione; 2, 4-dioxo-5-fluoropyrimidine; C4H3FN202 ; molecular weight 130.08; Chemical Abstract Registry Number 51-21-8
Table 4
Mouse Xenograft Test Results Growth Delay of Colon Tumor'4' With Irinotecan
(4) = Human HCT116 colon carcinoma
IRIN = Irinotecan; [1, 4 ' -Bipiperidine] -1 ' -carboxylic acid; (4S) -4 , ll-diethyl-3 ,4,12, 14-tetrahydro-4- hydroxy-3 , 14-dioxo-lH- pyrano [ 3 ' , 4 ' : 6 , 7 ] indolizino [ 1 , 2-b] quinolin-9-yl ester ; C33H38N406 ; Chemical Abstract Registry Number
97682 -44-5
Table 5
Mouse Xenograft Test Results Growth Delay of Non-Small Cell Lung Tumor'5' With Paclitaxel
(5) = Human H460 non-small cell lung carcinoma PACL = Paclitaxel; C47H5ιN0ι4; (CtR,βS)- β- (benzoylamino) -α-hydroxy-Benzenepropanoic acid (2aR,4S,4aS,6R,9S, IIS, 12S, 12aR, 12bS) -6 , 12b- bis (acetyloxy) -12- (benzoyloxy) -
2a, 3, 4, 4a, 5, 6,9, 10, 11, 12,12a, 12b-dodecanhydro-4 , 11- dihydroxy-4a, 8, 13, 13-tetramethyl-5-oxo-7 , 11-methano- lH-cyclodeca[3, 4]benz [1, 2-b] oxet-9-yl ester; Chemical Abstract Registry Number 33069-62-4 Table 6
Mouse Xenograft Test Results Growth Delay of Non-Small Cell Lung Tumor'6' With Carboplatin
(6) = Human H460 non-small cell lung carcinoma CARB = Carboplatin; (SP-4-2 ) -Diammine [1, 1- cyclobutanedicarboxylato (2-) -0, 0 ' ] platinum; 1,1- cyclobutanedicarboxylic acid platinum complex; C6Hι2N204Pt; molecular weight 371.25; Chemical Abstracts Registry Number 41575-94-4
Table 7
Mouse Xenograft Test Results Growth Delay of Non-Small Cell Lung Tumor'7' With Paclitaxel
(7) = Human Calu-6 non-small cell lung carcinoma PACL = Paclitaxel; C47H5ιN0ι4; (CCR,βS)- β- (benzoylamino) -α-hydroxy-Benzenepropanoic acid (2aR, 4S, 4aS, 6R, 9S, US, 12S, 12aR, 12bS) -6, 12b- bis (acetyloxy) -12- (benzoyloxy) -
2a, 3 , 4 , 4a, 5 , 6 , 9 , 10 , 11 , 12 , 12a, 12b-dodecanhydro-4 , 11- dihydroxy-4a, 8, 13, 13-tetramethyl-5-oxo-7 , 11-methano- lH-cyclodeca [3 , 4]benz [1, 2-b] oxet-9-yl ester; Chemical Abstract Registry Number 33069-62-4 Table 8
Mouse Xenograft Test Results Growth Delay of Non-Small Cell Lung Tumor'8' With Carboplatin
(8) = Human Calu-6 non-small cell lung carcinoma CARB = Carboplatin; (SP-4-2) -Diammine [1, 1- cyclobutanedicarboxylato (2-) -0,0' ] platinum; 1, 1- cyclobutanedicarboxylic acid platinum complex; C6Hι2N20Pt; molecular weight 371.25; Chemical Abstracts Registry Number 41575-94-4
Table 9
Mouse Xenograft Test Results Growth Delay of Breast Tumor'9' With Paclitaxel
(9) = Human MX-1 breast carcinoma
PACL = Paclitaxel; C47H5ιN0ι4; (CtR,βS)- β-
(benzoylamino) -α-hydroxy-Benzenepropanoic acid (2aR, 4S, 4aS, 6R, 9S, US, 12S, 12aR, 12bS) -6, 12b- bis (acetyloxy) -12- (benzoyloxy) -
2a, 3, 4, 4a, 5, 6, 9, 10, 11, 12,12a, 12b-dodecanhydro-4 , 11- dihydroxy-4a, 8,13, 13-tetramethyl-5-oxo-7 , 11-methano- lH-cyclodeca [3 , 4]benz [1, 2-b] oxet-9-yl ester; Chemical Abstract Registry Number 33069-62-4
Table 10
Mouse Xenograft Test Results Growth Delay of Breast Tumor'10' With Carboplatin
(10) = Human MX-1 breast carcinoma CARB = Carboplatin; (SP-4-2 ) -Diammine [1, 1- cyclobutanedicarboxylato (2-) -0, 0 ' ] platinum; 1, 1- cyclobutanedicarboxylic acid platinum complex; CeHι2N20Pt ; molecular weight 371.25; Chemical Abstracts Registry Number 41575-94-4
Table 11
Mouse Xenograft Test Results Growth Delay of Prostate Tumor'11' With Mitoxantrone
(11) = Human DU-145 prostate carcinoma MITO = Mitoxantrone; 1, 4-Dihydroxy-5 , 8-bis [ [2- [ (2- hydroxyethyl) amino] ethyl] amino] -9 , 10-anthracenedione; l,4-dihydroxy-5, 8-bis [ [2-[ (2- hydroxyethyl ) amino] ethyl] amino] -9, 10-anthraquinone; C22H28Nθ6; molecular weight 444.09; Chemical Abstracts Registry Number 65271-80-9 Table 12
Mouse Xenograft Test Results Growth Delay of Prostate Tumor'12' With Oxaliplatin
(12) = Human DU-145 prostate carcinoma OXAL = Oxaliplatin; (SP-4-2 )-[ (IR, 2R) -1, 2- cyclohexanediamine- N,KN' ] [ethanedioato (2-) -κ01,κ02] Platinum; C8Hι4N204Pt; Chemical Abstract Registry Number 61825-94-3
Table 13
Mouse Xenograft Test Results Growth Delay of Prostate Tumor'13' With Paclitaxel
(13) = Human DU-145 prostate carcinoma PACL = Paclitaxel; C47H5ιN0ι4; (αR,βS)- β- (benzoylamino) -α-hydroxy-Benzenepropanoic acid (2aR, 4S, 4aS, 6R, 9S, US, 12S, 12aR, 12bS) -6, 12b- bis (acetyloxy) -12- (benzoyloxy) -
2a, 3 , 4 , 4a, 5 , 6 , 9 , 10 , 11 , 12 , 12a, 12b-dodecanhydro-4 , 11- dihydroxy-4a, 8, 13 , 13-tetramethyl-5-oxo-7 , 11-methano- lH-cyclodeca [3 , 4]benz [1, 2-b] oxet-9-yl ester; Chemical Abstract Registry Number 33069-62-4.

Claims

What is claimed is:
1. A composition of matter comprising a therapeutically effective amount of a leukotriene (LTB4) antagonist and one or more anti-cancer agents.
2. The composition of claim 1 wherein the leukotriene (LTB4) antagonist is represented by the formula (I)
(1) wherein :
X is selected from the group consisting of,
(i) a five membered substituted or unsubstituted heterocyclic radical containing from 1 to 4 hetero atoms independently selected from sulfur, nitrogen or oxygen; and
(ii) a fused bicyclic radical wherein a carbocyclic group is fused to two adjacent carbon atoms of the five membered heterocyclic radical, (i); Y]_ is a bond or divalent linking group containing 1 to 9 atoms ;
Y2 and Y3 are divalent linking groups independently selected from -CH2-. -0- , or -S-;
Z is an Acidic Group;
Rl is Cι~C o alkyl, aryl, C3-C8 cycloalkyl, C2~Cχo alkenyl, C2-C-L0 alkynyl, c6_c20 aralkyl, C5-C20 alkaryl, cl"c10 haloalkyl, C5-C20 aryloxy, or alkoxy;
R2 is hydrogen, halogen, Cχ-Cχo haloalkyl, C^-CXQ alkoxy,
Cχ-Cχo alkyl, C3-C8 cycloalkyl, Acidic Group, or
- (CH2 ) 1-7- (Acidic Group) ;
R3 is hydrogen, halogen, Cχ-C]_o alkyl, aryl, Cχ-C]_Q haloalkyl, Cχ-Cχo alkoxy, C5-C20 aryloxy, or C3-C8 cycloalkyl;
R4 is C1-C4 alkyl, C3-C4 cycloalkyl,
- (CH2) 1-7- (C3-C4 cycloalkyl), C2-C4 alkenyl, C2-C4 alkynyl, benzyl , or aryl ; and
n is O, 1, 2, 3, 4, 5, or 6;
or a pharmaceutically acceptable salt, solvate, or prodrug derivative thereof, in combination with a therapeutically effective amount of an anti-cancer agent or a pharmaceutically acceptable salt, solvate, or prodrug derivative thereof.
3. The composition of claim 2 wherein X is a heterocyclic radical selected from the group consisting of substituents represented by the following formulae:
R10
where RIO is a radical selected from hydrogen or
C1-C4 alkyl; and Rll is a radical selected from hydrogen, halo, C]_-C]_o alkyl, Cχ-Cιo haloalkyl, C_-CIQ alkoxy, aryl, or C6-C20 aryloxy.
4. The composition of claim 2 wherein the Rl, R2 , R3 and R4 groups for substitution in formula (I) are selected from the following variables coded R01 thru R16
and;
the Yl, Y2 , and Y3 groups for substitution in formula (I) are selected from the following variables coded YOl thru Y27:
and;
the X and Z groups and the n variable for substitution in formula (I) are selected from the following variables coded XZnOl thru XZn24:
5. The composition of claim 2 wherein the leukotriene B4 antagonist is described by formula (II) :
wherein;
X2 is a heterocyclic radical selected from,
or
R21 is ethyl, 2-propen-l-yl, 3-propen-l-yl, n-propyl, iso-propyl, n-butyl, sec-butyl, or tert-butyl; and
R22 is hydrogen, n-butyl, sec-butyl, flouro, chloro, -CF3, or tert-butyl.
Z2 is the Acidic Group selected from carboxyl, tetrazolyl, or N-sulfonamidyl ;
or a salt, solvate or prodrug thereof.
6. The composition of claim 5 wherein the leukotriene antagonist is a compound selected from the following:
or an acid, salt, solvate or prodrug derivative thereof
7. The composition of claim 5 wherein the leukotriene antagonist is a compound selected from the following:
n acid, salt, solvate or prodrug derivative thereof
8. The composition of claim 1 the leukotriene (LTB4) antagonist is represented by a compound of the structure (Formula A) :
or a pharmaceutically acceptable base addition salt thereof, wherein:
Rl' is C1-C5 alkyl, C2-C5 alkenyl, C2-C5 alkynyl, C1-C4 alkoxy, (C1-C4 alkyl) thio, halo, or R2~substitutedphenyl ; each R2 ' and R3 > are each independently hydrogen, halo, hydroxy, C1-C4 alkyl, C1-C4 alkoxy, (C1-C4 alkyl) -(0)g S-, trifluoromethyl, or di- (C1-C3 alkyl ) amino ; X' is -0-, -S-, -C(=0), or -CH2-; Y' is -0- or -CH2-; or when taken together, -X'-Y'- is -CH=CH- or -C=C-; Z' is a straight or branched chain C1-C10 alkylidenyl; A' is a bond, -0-, -S-, -CH=CH- , or -CRaRjb-, where Ra and Rjb are each independently hydrogen, C1-C5 alkyl, or R7- substituted phenyl, or when taken together with the carbon atom to which they are attached form a C4-C8 cycloalkyl ring; R4' is Rg, or taken from one of the following formulae:
wherein : each R6 is independently -COOH, 5-tetrazolyl, -
C0N(R9)2- or -CONHS0 Rιo; each R7 is hydrogen, C1-C4 alkyl, C2-C5 alkenyl, C2-C5 alkynyl, benzyl, methoxy, -W-Rg, -T-G-R6. (C1-C4 alkyl) -T- (C1-C4 alkylidenyl) -0-, or hydroxy; R8 is hydrogen or halo; each Rg is independently hydrogen, phenyl, or C1-C4 alkyl, or when taken together with the nitrogen atom form a morpholino, piperidino, piperazino, or pyrrolidino group; RlO is C1-C4 alkyl or phenyl; Rll is R2, -W-R6, or -T-G-Rg; each W is a bond or a straight or branched chain divalent hydrocarbyl radical of one to eight carbon atoms; each G is a straight or branched chain divalent hydrocarbyl radical of one to eight carbon atoms; each T is a bond, -CH2-, -0-, -NH- , -NHC0-, -C(=0)-, or (0)g S-;
K is -C(=0)- or -CH(OH)-; each q is independently 0, 1, or 2 ; p is 0 or 1; and t is 0 or 1; provided when X is -0- or -S-, Y is not -0-; provided when A is -0- or -S-, R4 is not R6; and provided W is not a bond when p is 0; and the pharmaceutically-acceptable salts thereof.
9. The composition of claim 8 wherein R4 ' is selected from the following formulae:
10. The composition of claim 9 wherein R4'is:
11. The composition of claim 10 wherein said compound is selected from the group (A) to (KKKK) consisting of:
A) 2-Methyl-2- (lH-tetrazol-5-yl) -7- (2-ethyl-4- (4- fluorophenyl) -5-hydroxyphenoxy) heptane;
B) 2-Methyl-2- (lH-tetrazol-5-yl) -7- (2-ethyl-4- (3-fluorophenyl) -5-hydroxyphenoxy) heptane;
C) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- ( 4- dimethylaminocarbonylbutyloxy) phenyl ) propion ic acid; D) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionic acid; E) 3-(2-(3-(2-Ethyl-4-(4-fluorophenyl)-5- hydroxyphenoxy) propoxy) -6- (4- carboxybutyloxy) phenyl) propionic acid;
F) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- methoxyphenyl) propionic acid;
G) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- (4- (lH-tetrazol-5- yl) butyloxy) phenyl) propionic acid;
H) Methyl 3- (2- (4- (2-ethyl-4- (4-fluorophenyl) -
5-hydroxyphenoxy) - (1- butenyl) ) phenyl) propionate;
I) 3- (2- (4- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) - (1-butenyl) ) phenyl) propionic acid; J) 3-(2-(4-(2-Ethyl-4-(4-fluorophenyl)-5- hydroxyphenoxy) butyl ) phenyl) propionic acid;
K) 3- (2- (4- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) butyl) -6- methoxyphenyl) propionic acid;
L) Methyl 3- (2- (3- (2-ethyl-4- (4-fluorophenyl) -
5-hydroxyphenoxy) propoxy) -6- hydroxyphenyl ) propionate;
M) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- hydroxyphenyl) propionic acid; N) 3-(2-(3-(2-Ethyl-4-(4-fluorophenyl)-5- hydroxyphenoxy) propoxy) -6- (4- butyloxy) phenyl) propionic acid;
0) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) ropoxy) -6- ( 4- methylthiobutyloxy) phenyl) propionic acid; P) 3- (2- (3- (2, 4-Di- ( 4-fluorophenyl ) -5- hydroxyphenoxy) propoxy) -6- ( 4- carboxybutoxy) phenyl) propionic acid; Q) 6-Methyl-6-(lH-tetrazol-5-yl)-ll-(2-ethyl-4-
( 4-fluorophenyl ) -5-hydroxyphenoxy) ndecane;
R) N,N-Dimethyl-3- (2- (3- (2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionamide;
S) N-Methanesulfonyl-3- (2- (3- (2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionamide;
T) N-Phenylsulfonyl-3- (2- (3- (2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionamide; U) 3-(2-(3-(2-Butyl-4-(4-fluorophenyl)-5- hydroxyphenoxy) propoxy) phenyl ) propionic acid;
V) Ethyl 3- (2- (4- (2-ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) butyloxy) phenyl ) propionate ;
W) 3- (2- (4- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) butyloxy) phenyl ) propionic acid;
X) Methyl 3- (2- (3- (2-ethyl-4- (4-fluorophenyl) - 5-hydroxyphenoxy) propoxy) -6- (4- (methoxycarbonyl ) phenoxy) phenyl ) propionate ; Y) 3-(2-(3-(2-Ethyl-4-(4-fluorophenyl)-5- hydroxyphenoxy) propoxy) -6- ( 4- carboxyphenoxy) phenyl ) propionic acid;
Z) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -4- ( 4- carboxyphenoxy) phenyl) propionic acid;
AA) 3, 3-Dimethyl-3- (2- (3- (2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionic acid; BB) 2-Methyl-2- (lH-tetrazol-5-yl) -3- (2- (3- (2- ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propane ; CC) 2-Methyl-2-(lH-tetrazol-5-yl)-3-hydroxy-3-
(2- (3- (2-ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propane ;
DD) 3- (2- (3- (2-Bromo-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionic acid;
EE) 3- (2- (3- (2-Ethylthio-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionic acid;
FF) Methyl 3- ( 2-hydroxy-3- ( 4- methoxycarbonylbutyl) -6- (3- (2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionate ;
GG) 5- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -8- ( 4-carboxybuty 1 ) dihydrocoumarin;
HH) 2 -Phenyl-4-ethyl-5- [6- (2H-tetrazol-5-yl) -6- methylheptyloxyIphenol sodium salt;
II) 2- (4-Methylphenyl)-4-ethyl-5- [6-methyl-6- (2H-tetrazol-5-yl)heptyloxy] phenol disodium salt;
JJ) 2- (3-Methylphenyl) -4-ethyl-5- [6-methyl-6- (2H-tetrazol-5-yl) heptyloxy] phenol sodium salt;
KK) 2- (2-Methylphenyl) -4-ethyl-5- [6-methyl-6-
(2H-tetrazol-5-yl) heptyloxy] phenol disodium salt;
LL) 2- (4-Methoxyphenyl) -4-ethyl-5- [6-methyl-6- (2H-tetrazol-5-yl) heptyloxy] phenol sodium salt ; MM) 2- (3-Methoxyphenyl )-4-ethyl-5-[ 6-methyl-6-
(2H-tetrazol-5-yl) heptyloxy] phenol sodium salt; NN) 2- (4-Trifluoromethylphenyl) -4-ethyl-5- [6- methyl-6- (2H-tetrazol-5-yl) heptyloxy] phenol disodium salt; 00) 2- (3-Dimethylaminophenyl) -4-ethyl-5- [6- methyl-6- (2H-tetrazol-5-yl) heptyloxyIphenol disodium salt;
PP) 3- (5- (6- (4-Phenyl-5-hydroxy-2- ethylphenoxy) propoxy) -2-carboxymethyl-
1,2,3,4 -tetrahydronaphthalen-1 (2H) - one) propanoic acid;
QQ) 3- (5- (6- (4- (4-Fluorophenyl) -5-hydroxy-2- ethylphenoxy) propoxy) -2-carboxymeth yl-
1,2, 3, 4-tetrahydronaphthalen-1 (2H) - one) propanoic acid;
RR) 3- (4- (5- (4- (4-Fluorophenyl) -5-hydroxy-2- ethylphenoxy) propoxy) -2-carboxymeth yl-2,3- dihydroinden-1 (2H) -one) propanoic acid;
SS) 3, 3-Dimethyl-5- (3- (2-carboxyethyl) -4- (3- (4- fluorophenyl) -5-hydroxy-2- ethylphenoxy) propoxy) phenyl) -5-oxopentanoic acid;
TT) 7-[3-[ ( 5-Ethyl-2-hydroxy[ 1,1 '-biphenyl ] -4- yl) oxy] propoxy] -3, 4-dihydro-8-propyl-2H-l- benzopyran-2-carboxylic acid;
UU) 8-Propyl-7- [3- [4- (4-fluorophenyl) -2-ethyl-5- hydroxyphenoxy] propoxy] -3 , 4-dihydro-2H-l- benzopyran-2-carboxylic acid;
W) 2-[3-[3-[ ( 5-Ethyl-2-hydroxy[1,1' -biphenyl]- 4-yl) oxy] propoxy] -2-propylphenoxy] propanoic acid; WW) 2- (4-Chlorophenyl) -4-ethyl-5- [6-methyl-6-
(2H-tetrazol-5-yl) heptyloxy] phenol monosodium salt;
XX) 2- (3,5-Dichlorophenyl)-4-ethyl-5- [6-methyl- 6- (2H-tetrazol-5-yl) heptyloxy] phenol monosodium salt; YY) 3-[2-[3-[ (5-Ethyl-2-hydroxy[l,l' -biphenyl] - 4-yl) oxy] propoxy] -1-dibenzofuran] propanoic acid disodium salt; ZZ) -7-Carboxy-9-oxo-3- [3- (2-ethyl-5-hydroxy-4- phenylphenoxy) propoxy] -9H-xanthene-4- propanoic acid disodium salt monohydrate;
AAA) 2- [2-Propyl-3- [3- (2-ethyl-5-hydroxy-4- phenylphenoxy) propoxy] phenoxy] benzoic acid sodium salt hemihydrate;
BBB) 3- [3-(2-Ethyl-5-hydroxy-4- phenylphenoxy) propoxy] [1,1' -biphenyl ] -4- propanoic acid disodium salt monohydrate;
CCC) 5-Ethyl-4- [3- [2-propyl-3- [2- (2H-tetrazol-5- yl) phenoxy] phenoxy] propoxy] [1,1' -biphenyl ] - 2-ol disodium salt sesquihydrate;
DDD) 3-[4-[3-[ 3- (2-Ethyl-5-hydroxy-4- phenylphenoxy) propoxy] -9-oxo-9H- xanthene] ] propanoic acid sodium salt hemihydrate ;
EEE) 2-Fluoro-6- [2-propyl-3- [3- (2-ethyl-5- hydroxy-4- phenylphenoxy) propoxy] phenoxy] benzoic acid disodium salt;
FFF) 2- [2-Propyl-3- [3- [2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy] propoxy] phenoxy] benzoic acid sodium salt;
GGG) 3- [4- [7-Carboxy-9-oxo-3- [3- [2-ethyl-4- (4- fluorophenyl) -5-hydroxyphenoxy] propoxy] -9H- xanthene] ] propanoic acid disodium salt trihydrate;
HHH) 3- [4- [9-0x0-3- [3- [2-ethyl-4- (4- fluorophenyl) -5-hydroxyphenoxy] propoxy] -9H- xanthene] ] propanoic acid; III) 3- [2- [1- [2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy] propoxy] -4- (5-oxo-5- morpholinopentanamido) phenyl] propanoic acid;
JJJ) 2-Fluoro-6- [2-propyl-3- [3- [2-ethyl-5- hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] phenoxy] benzoic acid disodium salt hydrate;
KKK) 4-Fluoro-2- [2-propyl-3- [3- [2-ethyl-5- hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] phenoxy] benzoic acid;
LLL) 2- [2-Propyl-3- [5- [2-ethyl-5-hydroxy-4- (4- fluorophenyl ) phenoxy] pentoxy] phenoxy] benzoic acid; MMM) 2- [2-Propyl-3- [4- [2-ethyl-5-hydroxy-4- (4- fluorophenyl ) phenoxy] butoxy] phenoxy] benzoic acid sesquihydrate;
NNN) 2- [2- (2-Methylpropyl) -3- [3- [2 -ethyl-5- hydroxy-4- ( 4- fluorophenyl ) phenoxy] propoxy] phenoxy] benzoic acid;
000) 2- [2-Butyl-3- [3- [2-ethyl-5-hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] phenoxy] benzoic acid hydrate;
PPP) 2- [2- (Phenylmethyl) -3- [3- [2-ethyl-5-hydroxy- 4-(4- fluorophenyl ) phenoxy] propoxy] phenoxy] benzoic acid;
QQQ) 2- [2-Propyl-3- [3- [2-ethyl-5-hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] phenoxy] phenyla cetic acid;
RRR) 2- [2-Propyl-3- [3- [2-ethyl-5-hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] benzoyl ] benzoic acid; SSS ) 2 - [ [ 2 -Propyl-3- [ 3 - [ 2 -ethyl -5 -hydroxy-4- ( 4- fluorophenyl ) phenoxy] ropoxy] phenyl ] methyl ] b enzoic acid;
TTT) 2- [2-Propyl-3- [3- [2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy] propoxy] thiophenoxy] benzoic acid;
UUU) 2- [2-Propyl-3- [3- [2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy] propoxy] phenylsulfinyl] benzoi c acid;
VW) 2- [2-Propyl-3- [3- [2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy] propoxy] phenylsulfonyl] benzoi c acid hydrate;
WWW) 5- [3- [2- (1-Carboxy) ethyl] -4- [3- [2-ethyl-4- ( 4-fluorophenyl ) -5- hydroxyphenoxy] propoxy] phenyl ] -4-pentynoic acid disodium salt 0.4 hydrate;
XXX) 1-Phenyl-l- (lH-tetrazol-5-yl) -6- (2-ethyl-4- ( 4-fluorophenyl) -5-hydroxyphenoxy) hexane;
YYY) 1- (4- (Carboxymethoxy) phenyl) -1- (lH-tetrazol- 5-yl) -6- (2-ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) hexane;
ZZZ) 1- (4- (DimethylaminocarbonyImethoxy) phenyl) - 1- (lH-tetrazol-5-yl) -6- (2-ethyl-4- (4- fluorophenyl) -5-hydroxyphenoxy) hexane;
AAAA) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) -E-propenoic acid;
BBBB) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) -2-methy1-E- propenoic acid; CCCC) 5- (2- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) ethyl ) -1H- tetrazole;
DDDD) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -4- ( 4- carboxybutyloxy) phenyl) propionic acid;
EEEE) 5- [3- [4- (4-Fluorophenyl) -2-ethyl-5- hydroxyphenoxy] propoxy] -3 , 4-dihydro-2H-l- benzopyran-2-one;
FFFF) 3- (3-{3- [2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenyloxy] propoxy}phenyl ) propanoic acid;
GGGG) 3- (3-{3- [2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenyloxy] propoxy} -4-propylph enyl) propanoic acid sodium salt;
HHHH) 3- (4-{3- [2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenyloxy] propoxy} -3- propylphenyl) propanoic acid;
IIII) 3- (3-{3- [2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenyloxy] propoxy} -2- propylphenyl) propanoic acid;
JJJJ) 3-{3-[3-(2-Ethyl-5- hydroxyphenyloxy) propoxy] -2- propylphenyl }propanoic acid disodium salt; and
KKKK) 2- [3- [3- [2-Ethyl-5-hydroxy-4- (4- fluorophenyl ) henoxy] propoxy] benzoyl] benzoic acid disodium salt hemihydrate.
12. The composition of claim 8 wherein the leukotriene (LTB4) antagonist is a compound of the structure (Formula B) :
Formula B
namely, 2- [2-propy1-3- [3- [2-ethyl-5-hydroxy-4- (4- fluorophenyl) phenoxy] propoxy] phenoxy benzoic acid, and the pharmaceutically acceptable salts thereof.
13. The composition of claim 1 wherein the anti-cancer agent is selected from the group consisting of Busulfan, Carboplatin, Carmustine, Cisplatin, Cyclophosphamide, Dacarbazine, Ifosfamide, Lomustine, Streptozocin, Oxaliplatin, Temozolomide, Bleomycin, Dactinomycin, Daunorubicin, Doxorubicin, Idarubicin, Mitomycin-C, Plicamycin, Cryptophycin, Cytarabine, Floxuridine,
Fludarabine, 5-Fluorouracil, Hydroxyurea, 6-Mercaptopurine, Methotrexate, Thioguanine; Capecitabine, Aldesleukin, Interferon Alfa-2A, Interleukin-2 , Interleukin-12 (recombinant) , Interferon Alfa-2B (recombinant) , Interferon Alfa-n3, Interferon Gamma-IB, Herceptin interleukin-2, interleukin-12, Aminoglutethimide, Anastrozole, Flutamide, Goserelin, Leuprolide, Megestrol, Mitotane, Tamoxifen, Chlorambucil, Estramustine, Mechlorethamine, Melphalan, Thiotepa, Docetaxel, Etoposide, Irinotecan HCI, Paclitaxel, Teniposide, Topotecan, Vinblastine, Vincristine, Vinorelbine, Altretamine, Amifostine Asparaginase- Escherichia coli strain, BCG Live (Intravesical) , Cladribine, Leucovorin, Levamisole, Mitoxantrone, Pegaspargase, Pentostatin, and Procarbazine.
14. The composition of claim 13 wherein the anti- cancer agent is selected from the group consisting of Busulfan, Carboplatin, Carmustine, Cisplatin, Cyclophosphamide, Dacarbazine, Ifosfamide, Lomustine, Streptozocin, Oxaliplatin, Temozolomide, Bleomycin, Dactinomycin, Daunorubicin, Doxorubicin, Idarubicin, Mitomycin-C, Plicamycin, Cryptophycin, Cytarabine,
Floxuridine, Fludarabine, 5-Fluorouracil, Hydroxyurea, 6- Mercaptopurine, Methotrexate, Thioguanine; Capecitabine, Aldesleukin, Interferon Alfa-2A, Interleukin-2, Interleukin- 12 (recombinant) , Interferon Alfa-2B (recombinant) , Interferon Alfa-n3, Interferon Gamma-IB, Herceptin interleukin-2, interleukin-12, Aminoglutethimide, Anastrozole, Flutamide, Goserelin, Leuprolide, Megestrol, Mitotane, Tamoxifen, Chlorambucil, Estramustine, Mechlorethamine, Melphalan, Thiotepa, Docetaxel, Etoposide, Irinotecan HCI, Paclitaxel, Teniposide, Topotecan, Vinblastine, Vincristine, Vinorelbine, Altretamine, Amifostine Asparaginase-Escήerichia coli strain, BCG Live (Intravesical) , Cladribine, Leucovorin, Levamisole, Mitoxantrone, Pegaspargase, Pentostatin, and Procarbazine.
15. Use in the manufacture of a medicament for the treatment of cancer in mammals comprising a therapeutically effective amount of a leukotriene (LTB4) antagonist and one or more anti-cancer agents.
16. The use according to claim 15 wherein the leukotriene (LTB4) antagonist is represented by the formula (I)
(I) wherein:
X is selected from the group consisting of,
(i) a five membered substituted or unsubstituted heterocyclic radical containing from 1 to 4 hetero atoms independently selected from sulfur, nitrogen or oxygen; and
(ii) a fused bicyclic radical wherein a carbocyclic group is fused to two adjacent carbon atoms of the five membered heterocyclic radical, (i); Yl is a bond or divalent linking group containing 1 to 9 atoms;
Y2 and Y3 are divalent linking groups independently selected from -CH2-, -0-, or -S-;
Z is an Acidic Group;
Rl is C1-C10 alkyl, aryl, C3-C8 cycloalkyl, C2-C10 alkenyl, C2-C10 alkynyl, C5-C20 aralkyl, C5-C20 alkaryl, cl"c10 haloalkyl, C5-C20 aryloxy, or CI~CIQ alkoxy;
R2 is hydrogen, halogen, CI-CIQ haloalkyl, CI-CIQ alkoxy,
CI-CIQ alkyl, C3-C8 cycloalkyl, Acidic Group, or
- (CH2) 1-7- (Acidic Group);
R3 is hydrogen, halogen, CI-CIQ alkyl, aryl, CI-CIQ haloalkyl, CI-CIQ alkoxy, C -C20 aryloxy, or C3-C8 cycloalkyl;
R4 is C1-C4 alkyl, C3-C4 cycloalkyl,
- (CH2) 1-7- (C3-C4 cycloalkyl), C2-C4 alkenyl, C2-C4 alkynyl, benzyl , or aryl ; and
n is 0, 1, 2, 3, 4, 5, or 6;
or a pharmaceutically acceptable salt, solvate, or prodrug derivative thereof, in combination with a therapeutically effective amount of one or more anti-cancer agents.
17. The use according to claim 16 wherein X is a heterocyclic radical selected from the group consisting of substituents represented by the following formulae:
where RIO is a radical selected from hydrogen or
C1-C4 alkyl; and Rll is a radical selected from hydrogen, halo, CI-CIQ alkyl, CI-CIQ haloalkyl, CI-CIQ alkoxy, aryl, or Cg-C2o aryloxy.
18. The use according to claim 16 wherein the Rl, R2 , R3 and R4 groups for substitution in formula (I) are selected from the following variables coded ROl thru R16
and;
the Yl , Y2 , and Y3 groups for substitution in formula (I) are selected from the following variables coded YOl thru Y27:
and; the X and Z groups and the n variable for substitution in formula (I) are selected from the following variables coded XZnOl thru XZn24:
19. A use according to claim 16 wherein the leukotriene B4 antagonist is described by formula (ID :
wherein;
X2 is a heterocyclic radical selected from,
or
R R2211 iiss eetthhyyll,, 22--pprrooppeenn--ll--yyll,, 33--pprrooppeenn--ll--yyll,, nn-propyl, iso-propyl, n-butyl, sec-butyl, or tert-butyl; and
R22 is hydrogen, n-butyl, sec-butyl, flouro, chloro, -CF3, or tert-butyl. Z2 is the Acidic Group selected from carboxyl, tetrazolyl, or N-sulfonamidyl;
or a salt, solvate or prodrug thereof.
20. The use according to claim 19, wherein the leukotriene antagonist is a compound selected from the following:
10
or an acid, salt, solvate or prodrug derivative thereof,
21. The use according to claim 20 wherein the leukotriene antagonist is a compound selected from the following:
or an acid, salt, solvate or prodrug derivative thereof.
22. Use in the manufacture of a medicament for the treatment of cancer in mammals comprising a therapeutically effective amount of a leukotriene (LTB4) antagonist represented by a compound of the structure (Formula A) :
Formula A or a pharmaceutically acceptable base addition salt thereof, wherein:
Rl' is C1-C5 alkyl, C2-C5 alkenyl, C2-C5 alkynyl, C1-C4 alkoxy, (C1-C4 alkyl) thio, halo, or R2~substitutedphenyl ; each R2 ' and R3 ' are each independently hydrogen, halo, hydroxy, C1-C4 alkyl, C1-C4 alkoxy, (C1-C4 alkyl) -(0)g S-, trifluoromethy1, or di- (C1-C3 alkyl)amino; X' is -0-, -S-, -C(=0), or -CH2-; Y' is -0- or -CH2-; or when taken together, -X'-Y'- is -CH=CH- or -C=C-; Z' is a straight or branched chain C1-C10 alkylidenyl; A' is a bond, -0- , -S-, -CH=CH-, or -CRaR - where Ra and Rjb are each independently hydrogen, C1-C5 alkyl, or R7- substituted phenyl, or when taken together with the carbon atom to which they are attached form a C4-C8 cycloalkyl ring;
R4 » is R5, or taken from one of the following formulae
wherein: each R6 is independently -COOH, 5-tetrazolyl, - CON(Rg)2. or -CONHS0 Rιo; each R7 is hydrogen, C1-C4 alkyl, C2-C5 alkenyl, C2-C5 alkynyl, benzyl, methoxy, -W-Rβ. -T-G-R6. (C1-C4 alkyl) -T- (C1-C4 alkylidenyl) -0-, or hydroxy;
R8 is hydrogen or halo; each Rg is independently hydrogen, phenyl, or C1-C4 alkyl, or when taken together with the nitrogen atom form a morpholino, piperidino, piperazino, or pyrrolidino group;
RlQ is C1-C4 alkyl or phenyl; Rll is R2, -W-R6, or -T-G-R6; each W is a bond or a straight or branched chain divalent hydrocarbyl radical of one to eight carbon atoms; each G is a straight or branched chain divalent hydrocarbyl radical of one to eight carbon atoms; each T is a bond, -CH2-, -0- , -NH- , -NHC0-, -C(=0)-, or (0)g S-;
K is -C(=0)- or -CH(OH)-; each q is independently 0, 1, or 2; p is 0 or 1; and t is 0 or 1; provided when X is -0- or -S-, Y is not -0- ; provided when A is -0- or -S-, R4 is not Rζ ; and provided W is not a bond when p is 0; in combination with a therapeutically effective amount of one or more anti-cancer agents.
23. The use of claim 22 wherein R4'is selected from the following formulae:
24. The use of claim 23 wherein R4'is:
25. The use according to claim 24 wherein said compound lected from the group (A) to (KKKK) consisting of:
A) 2-Methyl-2- (lH-tetrazol-5-yl) -7- (2-ethyl-4- (4- fluorophenyl) -5-hydroxyphenoxy) heptane;
B) 2-Methyl-2- (lH-tetrazol-5-yl) -7- (2-ethyl-4- (3-fluorophenyl) -5-hydroxyphenoxy) heptane;
C) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- (4- dimethylaminocarbonylbutyloxy) phenyl ) propion ic acid;
D) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionic acid;
E) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- (4- carboxyb tyloxy) phenyl) propionic acid;
F) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- methoxyphenyl) propionic acid;
G) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- (4- (lH-tetrazol-5- yl ) butyloxy) phenyl ) propionic acid;
H) Methyl 3- (2- (4- (2-ethyl-4- (4-fluorophenyl) - 5-hydroxyphenoxy) - (1- butenyl ) ) phenyl ) propionate; I) 3- (2- (4- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) - (1-butenyl ) ) phenyl ) propionic acid;
J) 3- (2- (4- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) butyl ) phenyl ) propionic acid;
K) 3- (2- (4- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) butyl ) -6- methoxyphenyl ) propionic acid;
L) Methyl 3- (2- (3- (2-ethyl-4- (4-fluorophenyl) - 5-hydroxyphenoxy) propoxy) -6- hydroxyphenyl) propionate;
M) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- hydroxyphenyl) propionic acid;
N) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- (4- butyloxy) phenyl) propionic acid; 0) 3-(2-(3-(2-Ethyl-4-(4-fluorophenyl)-5- hydroxyphenoxy) propoxy) -6- ( 4- methylthiobutyloxy) phenyl) propionic acid;
P) 3- (2- (3- (2 , 4-Di- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) - 6- ( 4- carboxybutoxy) phenyl ) propionic acid;
Q) 6-Methyl-6- (lH-tetrazol-5-yl) -11- (2-ethyl-4- ( 4- fluorophenyl ) -5 -hydroxyphenoxy) ndecane ;
R) N,N-Dimethyl-3- (2- (3- (2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionamide ; S) N-Methanesulfonyl-3- (2- (3- (2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionamide;
T) N-Phenylsulfonyl-3- (2- (3- (2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionamide ; U) 3- (2- (3- (2-Butyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionic acid; V) Ethyl 3-(2-(4-(2-ethyl-4-(4-fluorophenyl)-5- hydroxyphenoxy) butyloxy) phenyl ) propionate;
W) 3- (2- (4- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) butyloxy) phenyl ) propionic acid;
X) Methyl 3- (2- (3- (2-ethyl-4- (4-fluorophenyl) - 5-hydroxyphenoxy) propoxy) -6- (4- (methoxycarbonyl ) phenoxy) phenyl ) propionate;
Y) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- (4- carboxyphenoxy) phenyl) propionic acid; Z) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -4- (4- carboxyphenoxy) phenyl ) propionic acid;
AA) 3,3-Dimethyl-3-(2- (3- (2-ethyl-4- (4- fluorophenyl ) -5- hydroxyphenoxy) propoxy) phenyl ) propionic acid;
BB) 2-Methyl-2- (lH-tetrazol-5-yl) -3- (2- (3- (2- ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl) propane ;
CC) 2-Methyl-2- (lH-tetrazol-5-yl) -3-hydroxy-3- (2- (3- (2-ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propane ;
DD) 3- (2- (3- (2-Bromo-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionic acid;
EE) 3- (2- (3- (2-Ethylthio-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionic acid; FF) Methyl 3- (2-hydroxy-3- (4- methoxycarbonylbutyl ) -6- (3- (2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionate; GG) 5- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -8- (4-carboxybuty 1) dihydrocoumarin;
HH) 2-Phenyl-4-ethyl-5- [6- (2H-tetrazol-5-yl) -6- methylheptyloxy] phenol sodium salt;
II) 2- (4-Methylphenyl) -4-ethyl-5- [ 6-methyl-6- (2H-tetrazol-5-yl)heptyloxy]phenol disodium salt;
JJ) 2- (3-Methylphenyl) -4-ethyl-5- [6-methyl-6- (2H-tetrazol-5-yl) eptyloxy] phenol sodium salt;
KK) 2- (2-Methylphenyl) -4-ethyl-5- [6-methyl-6-
(2H-tetrazol-5-yl) heptyloxy] phenol disodium salt;
LL) 2- (4-Methoxyphenyl) -4-ethyl-5- [6-methyl-6-
(2H-tetrazol-5-yl) heptyloxy] phenol sodium salt; MM) 2- ( 3-Methoxyphenyl )-4-ethyl-5-[6-methyl-6-
(2H-tetrazol-5-yl) heptyloxy] phenol sodium salt;
NN) 2- (4-Trifluoromethylphenyl) -4-ethyl-5- [6- methyl-6- (2H-tetrazol-5-yl) heptyloxy] phenol disodium salt;
00) 2- (3-Dimethylaminophenyl) -4-ethyl-5- [6- methyl-6- (2H-tetrazol-5-yl) heptyloxy] phenol disodium salt;
PP) 3-(5-(6-(4-Phenyl-5-hydroxy-2- ethylphenoxy) propoxy) -2-carboxymethyl- 1,2,3,4 -tetrahydronaphthalen-1 (2H) - one) propanoic acid;
QQ) 3- (5- (6- (4- (4-Fluorophenyl) -5-hydroxy-2- ethylphenoxy) propoxy) -2-carboxymeth yl- 1,2,3, 4-tetrahydronaphthalen-1 (2H) - one) propanoic acid; RR) 3- (4- (5- (4- (4-Fluorophenyl) -5-hydroxy-2- ethylphenoxy) propoxy) -2-carboxymeth yl-2 , 3- dihydroinden-1 (2H) -one) propanoic acid; SS) 3,3-Dimethyl-5-(3-(2-carboxyethyl)-4-(3-(4- fluorophenyl) -5-hydroxy-2- ethylphenoxy) propoxy) phenyl ) -5-oxopentanoic acid; TT) 7- [3- [ (5-Ethyl-2-hydroxy[l,l" -biphenyl] -4- yl) oxy]propoxy] -3, 4-dihydro-8-propyl-2H-l- benzopyran-2-carboxylic acid;
UU) 8-Proρyl-7- [3- [4- (4-fluorophenyl) -2-ethyl-5- hydroxyphenoxy] propoxy] -3 , 4-dihydro-2H-l- benzopyran-2-carboxylic acid;
W) 2-[3-[3-[ ( 5-Ethyl-2-hydroxy [1,1' -biphenyl]- 4-yl) oxy] propoxy] -2-propylphenoxy] propanoic acid;
WW) 2- (4-Chlorophenyl) -4-ethyl-5- [6-methyl-6- (2H-tetrazol-5-yl) heptyloxy] phenol monosodium salt;
XX) 2- (3, 5-Dichlorophenyl)-4-ethyl-5- [6-methyl- 6- (2H-tetrazol-5-yl) heptyloxy] phenol monosodium salt; YY) 3- [2- [ 3- [ (5-Ethyl-2-hydroxy [1,1' -biphenyl]-
4-yl) oxy] propoxy] -1-dibenzofuran] propanoic acid disodium salt;
ZZ) 7-Carboxy-9-oxo-3- [3- (2-ethyl-5-hydroxy-4- phenylphenoxy) propoxy] -9H-xanthene-4- propanoic acid disodium salt monohydrate;
AAA) 2- [2-Propyl-3- [3- ( 2-ethyl-5-hydroxy-4- phenylphenoxy) propoxy] phenoxy] benzoic acid sodium salt hemihydrate;
BBB) 3-[3-(2-Ethyl-5-hydroxy-4- phenylphenoxy) propoxy] [1,1' -biphenyl ] -4- propanoic acid disodium salt monohydrate; CCC) 5-Ethyl-4- [3- [2-propyl-3- [2- (2H-tetrazol-5- y1 ) phenoxy] phenoxy] propoxy] [1,1' -biphenyl] - 2-ol disodium salt sesquihydrate;
DDD) 3-[4-[3-[ 3-(2-Ethyl-5-hydroxy-4- phenylphenoxy) propoxy] -9-oxo-9H- xanthene] ] propanoic acid sodium salt hemihydrate;
EEE) 2-Fluoro-6- [2-propyl-3- [3- (2-ethyl-5- hydroxy-4- phenylphenoxy) propoxy] phenoxy] benzoic acid disodium salt;
FFF) 2- [2-Propyl-3- [3- [2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy] propoxy] phenoxy] benzoic acid sodium salt;
GGG) 3- [4- [7-Carboxy-9-oxo-3- [3- [2-ethyl-4- (4- fluorophenyl) -5-hydroxyphenoxy] propoxy] -9H- xanthene] ] propanoic acid disodium salt trihydrate ;
HHH) 3- [4- [9-OXO-3- [3- [2-ethyl-4- (4- fluorophenyl) -5-hydroxyphenoxy] propoxy] -9H- xanthene] ] propanoic acid; III) 3- [2- [l-[2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy] propoxy] -4- (5-oxo-5- morpholinopentanamido) phenyl] propanoic acid;
JJJ) 2-Fluoro-6- [2-ρropyl-3- [3- [2-ethyl-5- hydroxy-4- ( 4- fluorophenyl ) phenoxy] propoxy] phenoxy] benzoic acid disodium salt hydrate;
KKK) 4-Fluoro-2- [2-propyl-3- [3- [2-ethyl-5- hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] phenoxy] benzoic acid; LLL) 2- [ 2-Propyl-3- [ 5- [ 2 -ethyl -5 -hydroxy- 4- ( 4- fluorophenyl ) phenoxy] pentoxy] phenoxy] benzoic acid;
MMM) 2- [2-Propyl-3- [4- [2-ethyl-5-hydroxy-4- (4- fluorophenyl) phenoxy] butoxy] phenoxy] benzoic acid sesquihydrate; NNN) 2- [2-(2-Methylpropyl)-3-[3-[2-ethyl-5- hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] phenoxy] benzoic acid; 000) 2-[2-Butyl-3-[3-[2-ethyl-5-hydroxy-4-(4- fluorophenyl ) phenoxy] propoxy] phenoxy] benzoic acid hydrate;
PPP) 2- [2- (Phenylmethyl) -3- [3- [2-ethyl-5-hydroxy- 4-(4- fluorophenyl ) phenoxy] propoxy] phenoxy] benzoic acid;
QQQ) 2- [2-Propyl-3- [3- [2-ethyl-5-hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] henoxy] henyla cetic acid;
RRR) 2- [2-Propyl-3- [3- [2 -ethyl- 5 -hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] benzoyl ] benzoic acid;
SSS) 2- [ [2-Propyl-3- [3- [2-ethyl-5-hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] phenyl] methyl ] b enzoic acid;
TTT) 2- [2-Propyl-3- [3- [2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy] propoxy] thiophenoxy] benzoic acid;
UUU) 2- [2-Propyl-3- [3- [2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy] propoxy] phenylsulfinyl ] benzoi c acid; VW) 2- [2-Propyl-3- [3- [2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy] propoxy] phenylsulfonyl] benzoi c acid hydrate;
WWW) 5- [3- [2- (1-Carboxy) ethyl] -4- [3- [2-ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy] propoxy] phenyl ] -4-pentynoic acid disodium salt 0.4 hydrate;
XXX) 1-Phenyl-l- (lH-tetrazol-5-yl) -6- (2-ethyl-4- (4-fluorophenyl ) -5-hydroxyphenoxy) hexane ; YYY) 1- (4- (Carboxymethoxy) phenyl ) -1- (lH-tetrazol-
5-yl) -6- (2-ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) hexane;
ZZZ) 1- (4- (DimethylaminocarbonyImethoxy) phenyl) - 1- (lH-tetrazol-5-yl) -6- (2-ethyl-4- (4- fluorophenyl ) -5-hydroxyphenoxy) hexane;
AAAA) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) -E-propenoic acid;
BBBB) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) -2-methyl-E- propenoic acid;
CCCC) 5- (2- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) ethyl) -1H- tetrazole; DDDD) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -4- (4- carboxybutyloxy) phenyl) propionic acid;
EEEE) 5- [3- [4- (4-Fluorophenyl) -2-ethyl-5- hydroxyphenoxy] propoxy] -3 , 4-dihydro-2H-l- benzopyran-2-one; FFFF) 3- (3-{3- [2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenyloxy] propoxy}phenyl ) propanoic acid;
GGGG) 3- (3-{3- [2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenyloxy] propoxy} -4-propylph enyl) propanoic acid sodium salt;
HHHH) 3- (4-{3- [2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenyloxy] propoxy} -3- propylphenyl) propanoic acid;
IIII) 3- (3-{3-[2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenyloxy] propoxy} -2- propylphenyl) propanoic acid;
JJJJ) 3-{3-[3-(2-Ethyl-5- hydroxyphenyloxy) propoxy] -2- propylphenyl}propanoic acid disodium salt; and
KKKK) 2- [3- [3- [2-Ethyl-5-hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] benzoyl] benzoic acid disodium salt hemihydrate.
26. The use according to claim 22 wherein the leukotriene (LTB4) antagonist is a compound of the structure (Formula B) :
Formula B namely, 2 - [ 2-propyl-3 - [ 3 - [ 2 -ethyl- 5 -hydroxy-4- ( 4- fluorophenyl) phenoxy] propoxy] phenoxy benzoic acid, and the pharmaceutically acceptable salts thereof.
27. The use of claim 15 wherein the anti-cancer agent is selected from the group consisting of Busulfan, Carboplatin, Carmustine, Cisplatin, Cyclophosphamide, Dacarbazine, Ifosfamide, Lomustine, Streptozocin, Oxaliplatin, Temozolomide, Bleomycin, Dactinomycin, Daunorubicin, Doxorubicin, Idarubicin, Mitomycin-C, Plicamycin, Cryptophycin, Cytarabine, Floxuridine, Fludarabine, 5-Fluorouracil, Hydroxyurea, 6-Mercaptopurine, Methotrexate, Thioguanine; Capecitabine, Aldesleukin, Interferon Alfa-2A, Interleukin-2, Interleukin-12 (recombinant) , Interferon Alfa-2B (recombinant) , Interferon Alfa-n3, Interferon Gamma-IB, Herceptin interleukin-2, interleukin-12, Aminoglutethimide, Anastrozole, Flutamide, Goserelin, Leuprolide, Megestrol, Mitotane, Tamoxifen, Chlorambucil, Estramustine, Mechlorethamine, Melphalan, Thiotepa, Docetaxel, Etoposide, Irinotecan HCI, Paclitaxel, Teniposide, Topotecan, Vinblastine, Vincristine, Vinorelbine, Altretamine, Amifostine Asparaginase- Escherichia coli strain, BCG Live (Intravesical) , Cladribine, Leucovorin, Levamisole, Mitoxantrone, Pegaspargase, Pentostatin, and Procarbazine.
28. The use of claim 26 wherein the anti-cancer agent is selected from the group consisting of Busulfan, Carboplatin, Carmustine, Cisplatin, Cyclophosphamide, Dacarbazine, Ifosfamide, Lomustine, Streptozocin,
Oxaliplatin, Temozolomide, Bleomycin, Dactinomycin, Daunorubicin, Doxorubicin, Idarubicin, Mitomycin-C, Plicamycin, Cryptophycin, Cytarabine, Floxuridine, Fludarabine, 5-Fluorouracil, Hydroxyurea, 6-Mercaptopurine, Methotrexate, Thioguanine; Capecitabine, Aldesleukin, Interferon Alfa-2A, Interleukin-2, Interleukin-12 (recombinant) , Interferon Alfa-2B (recombinant) , Interferon Alfa-n3, Interferon Gamma-IB, Herceptin interleukin-2, interleukin-12, Aminoglutethimide, Anastrozole, Flutamide, Goserelin, Leuprolide, Megestrol, Mitotane, Tamoxifen, Chlorambucil, Estramustine, Mechlorethamine, Melphalan, Thiotepa, Docetaxel, Etoposide, Irinotecan HCI, Paclitaxel, Teniposide, Topotecan, Vinblastine, Vincristine, Vinorelbine, Altretamine, Amifostine Asparaginase- Escherichia coli strain, BCG Live (Intravesical) , Cladribine, Leucovorin, Levamisole, Mitoxantrone, Pegaspargase, Pentostatin, and Procarbazine.
29. A method of treating cancer in a human patient by administering to said patient a composition comprising a therapeutically effective amount of a leukotriene (LTB4) antagonist and one or more anti-cancer agents.
30. The method according to claim 29 wherein the leukotriene (LTB4) antagonist is represented by the formula (I)
(i) wherein :
X is selected from the group consisting of,
(i) a five membered substituted or unsubstituted heterocyclic radical containing from 1 to 4 hetero atoms independently selected from sulfur, nitrogen or oxygen; and
(ii) a fused bicyclic radical wherein a carbocyclic group is fused to two adjacent carbon atoms of the five membered heterocyclic radical, (i);
Yi is a bond or divalent linking group containing 1 to 9 atoms;
Y2 and Y3 are divalent linking groups independently selected from -CH2-, -0-, or -S-;
Z is an Acidic Group;
Rl is C1-C10 alkyl, aryl, C3-C8 cycloalkyl, C2-C1Q alkenyl, c2"c10 alkynyl, C6-C2o aralkyl, C6-C2o alkaryl, Cl-C10 haloalkyl, C6-C20 aryloxy, or CI-CIQ alkoxy; R2 is hydrogen, halogen, CI-CIQ haloalkyl, CI-CIQ alkoxy, cl~c10 alkyl, C3-C8 cycloalkyl, Acidic Group, or - (CH2) 1-7- (Acidic Group);
R3 is hydrogen, halogen, C -C Q alkyl, aryl, CI-CIQ haloalkyl, CI~CIQ alkoxy, C5-C20 aryloxy, or C3-C8 cycloalkyl;
R4 is C1-C4 alkyl, C3-C4 cycloalkyl, - (CH2) 1-7- (C3-C4 cycloalkyl), C2-C4 alkenyl, C2-C4 alkynyl, benzyl , or aryl ; and
n is O, 1, 2, 3, 4, 5, or 6;
or a pharmaceutically acceptable salt, solvate, or prodrug derivative thereof, in combination with a therapeutically effective amount of one or more anti-cancer agents.
31. The method according to claim 30 wherein X is a heterocyclic radical selected from the group consisting of substituents represented by the following formulae:
where RIO is a radical selected from hydrogen or
C1-C4 alkyl; and Rll is a radical selected from hydrogen, halo, CI-CIQ alkyl, CI-CIQ haloalkyl, CI-CIQ alkoxy, aryl, or C6-C2Q aryloxy.
32. The method according to claim 31 wherein the Rl, R2 , R3 and R4 groups for substitution in formula (I) are selected from the following variables coded ROl thru R16
and;
the Yl, Y2 , and Y3 groups for substitution in formula (I) are selected from the following variables coded YOl thru Y27:
and; the X and Z groups and the n variable for substitution in formula (I) are selected from the following variables coded XZnOl thru XZn24:
33. A method according to claim 30 wherein the leukotriene B4 antagonist is described by formula (II) :
wherein;
X2 is a heterocyclic radical selected from,
or
R21 is ethyl, 2-propen-l-yl, 3-propen-l-yl, n-propyl, iso-propyl, n-butyl, sec-butyl, or tert-butyl; and
R22 is hydrogen, n-butyl, sec-butyl, flouro, chloro, -CF3, or tert-butyl.
Z2 is the Acidic Group selected from carboxyl, tetrazolyl, or N-sulfonamidyl; or a salt, solvate or prodrug thereof.
34. The method according to claim 33, wherein the leukotriene antagonist is a compound selected from the following:
10
or an acid, salt, solvate or prodrug derivative thereof,
35. The method according to claim 34 wherein the leukotriene antagonist is a compound selected from the following:
or an acid, salt, solvate or prodrug derivative thereof.
36. The method of claim 29 wherein the leukotriene
(LTB4) antagonist represented by a compound of the structure (Formula A) :
Formula A or a pharmaceutically acceptable base addition salt thereof, wherein:
R ' is C1-C5 alkyl, C2-C5 alkenyl, C2-C5 alkynyl, C1-C4 alkoxy, (C1-C4 alkyl) thio, halo, or R2~substitutedphenyl ; each R2 ' and R3 > are each independently hydrogen, halo, hydroxy, C1-C4 alkyl, C1-C4 alkoxy, (C1-C4 alkyl) - (0) g S- , trifluoromethyl, or di- (C1-C3 alkyl) amino; X' is -0-, -S-, -C(=0), or -CH2-; Y' is -0- or -CH2-; or when taken together, -X'-Y'- is -CH=CH- or -C=C-; Z' is a straight or branched chain C1-C10 alkylidenyl; A' is a bond, -0-, -S-, -CH=CH-, or -CRaRj-, where Ra and R& are each independently hydrogen, C1-C5 alkyl, or R7- substituted phenyl, or when taken together with the carbon atom to which they are attached form a C4-C8 cycloalkyl ring;
R4 ' is Rg, or taken from one of the following formulae:
wherein: each R6 is independently -COOH, 5-tetrazolyl, - C0N(R9)2, or -CONHSO2R10; each R7 is hydrogen, C1-C4 alkyl, C2-C5 alkenyl, C2-C5 alkynyl, benzyl, methoxy, -W-R6, -T-G-R6, (C1-C4 alkyl) -T- (C1-C4 alkylidenyl) -0-, or hydroxy;
R8 is hydrogen or halo; each R9 is independently hydrogen, phenyl, or C1-C4 alkyl, or when taken together with the nitrogen atom form a morpholino, piperidino, piperazino, or pyrrolidino group;
RlO is C1-C4 alkyl or phenyl; Rll is R2, -W-R6, or -T-G-R6; each is a bond or a straight or branched chain divalent hydrocarbyl radical of one to eight carbon atoms; each G is a straight or branched chain divalent hydrocarbyl radical of one to eight carbon atoms; each T is a bond, -CH2-, -0- , -NH-, -NHC0-, -C(=0)-, or (0)g S-;
K is -C(=0)- or -CH(OH)-; each q is independently 0, 1, or 2; p is 0 or 1 ; and t is 0 or 1; provided when X is -0- or -S-, Y is not -0- ; provided when A is -0- or -S-, R4 is not R6 ; and provided is not a bond when p is 0; in combination with a therapeutically effective amount of one or more anti-cancer agents.
37. The method of claim 36 wherein R4'is selected from the following formulae:
38. The composition of claim 37 wherein R4'is:
39. The method of claim 36 wherein said compound is selected from the group (A) to (KKKK) consisting of:
A) 2-Methyl-2- (lH-tetrazol-5-yl) -7- (2-ethyl-4- (4- fluorophenyl) -5-hydroxyphenoxy) heptane;
B) 2-Methyl-2- (lH-tetrazol-5-yl) -7- (2-ethyl-4-
( 3-fluorophenyl ) -5-hydroxyphenoxy) heptane ;
C) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- (4- dimethylaminocarbonylbutyloxy) phenyl ) propion ic acid;
D) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionic acid;
E) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- ( 4- carboxybutyloxy) phenyl) propionic acid;
F) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- methoxyphenyl) propionic acid; G) 3-(2-(3-(2-Ethyl-4-(4-fluorophenyl)-5- hydroxyphenoxy) propoxy) -6- (4- (lH-tetrazol-5- yl ) butyloxy) phenyl ) propionic acid;
H) Methyl 3- (2- (4- (2-ethyl-4- (4-fluorophenyl) - 5-hydroxyphenoxy) - (1- butenyl) ) phenyl) propionate; I) 3- (2- (4- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) - (1-butenyl ) ) phenyl ) propionic acid;
J) 3- (2- (4- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) butyl ) phenyl ) propionic acid;
K) 3- (2- (4- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) butyl ) -6- methoxyphenyl) propionic acid;
L) Methyl 3- (2- (3- (2-ethyl-4- (4-fluorophenyl) - 5-hydroxyphenoxy) propoxy) -6- hydroxyphenyl ) propionate;
M) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- hydroxyphenyl) propionic acid;
N) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- (4- butyloxy) phenyl) propionic acid; 0) 3-(2-(3-(2-Ethyl-4-(4-fluorophenyl)-5- hydroxyphenoxy) propoxy) -6- (4- methylthiobutyloxy) phenyl) propionic acid;
P) 3- (2- (3- (2 , 4-Di- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -6- ( 4- carboxybutoxy) phenyl) propionic acid;
Q) 6-Methyl-6- (lH-tetrazol-5-yl) -11- (2-ethyl-4- ( 4- fluorophenyl ) -5-hydroxyphenoxy) undecane ;
R) N,N-Dimethyl-3- (2- (3- (2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionamide ; S) N-Methanesulfonyl-3-(2-(3-(2-ethyl-4-(4- fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionamide; T) N-Phenylsulfonyl-3- (2- (3- (2-ethyl-4- (4- fluorophenyl ) -5- hydroxyphenoxy) propoxy) phenyl ) propionamide ;
U) 3- (2- (3- ( 2 -Butyl-4-( 4-fluorophenyl) -5- hydroxyphenoxy) ropoxy) phenyl ) propionic acid; V) Ethyl 3-(2-(4-(2-ethyl-4-(4-fluorophenyl)-5- hydroxyphenoxy) butyloxy) phenyl ) propionate ;
W) 3- (2- (4- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) butyloxy) phenyl ) propionic acid;
X) Methyl 3- (2- (3- (2-ethyl-4- (4-fluorophenyl) - 5-hydroxyphenoxy) propoxy) -6- ( 4- (methoxycarbonyl ) phenoxy) phenyl ) propionate ;
Y) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) - 6- ( 4- carboxyphenoxy) phenyl ) propionic acid; Z) 3-(2-(3-(2-Ethyl-4-(4-fluorophenyl)-5- hydroxyphenoxy) propoxy) -4- (4- carboxyphenoxy) phenyl) propionic acid;
AA) 3, 3-Dimethyl-3- (2- (3- (2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionic acid;
BB) 2-Methyl-2- (lH-tetrazol-5-yl) -3- (2- (3- (2- ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propane ,-
CO 2-Methyl-2- ( IH-tetrazol-5-yl) -3 -hydroxy-3- (2- (3- (2-ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propane ;
DD) 3- (2- (3-(2-Bromo-4-(4-fluorophenyl)-5- hydroxyphenoxy) propoxy) phenyl ) propionic acid;
EE) 3- (2- (3- (2-Ethylthio-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionic acid; FF) Methyl 3- ( 2-hydroxy-3 - ( 4- methoxycarbonylbutyl ) -6- (3- (2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) propionate ;
GG) 5- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -8- (4-carboxybuty 1 ) dihydrocoumarin ;
HH) 2-Phenyl-4-ethyl-5- [6- (2H-tetrazol-5-yl) -6- methylheptyloxy] phenol sodium salt;
II) 2- (4-Methylphenyl) -4-ethyl-5- [ 6-methyl-6- (2H-tetrazol-5-yl) heptyloxy] phenol disodium salt;
JJ) 2-(3-Methylphenyl) -4-ethyl-5- [6-methyl-6- ( 2H-tetrazol-5-yl) heptyloxyIphenol sodium salt;
KK) 2- (2-Methylphenyl) -4-ethyl-5- [6-methyl-6-
(2H-tetrazol-5-yl) heptyloxy] phenol disodium salt;
LL) 2- (4-Methoxyphenyl) -4-ethyl-5- [6-methyl-6-
(2H-tetrazol-5-yl) heptyloxy] phenol sodium salt ; MM) 2- ( 3 -Methoxyphenyl )-4-ethyl- 5- [6-methyl-6-
(2H-tetrazol-5-yl) heptyloxy] phenol sodium salt ;
NN) 2- (4-Trifluoromethylphenyl) -4-ethyl-5- [6- methyl-6- (2H-tetrazol-5-yl)heptyloxy]phenol disodium salt;
00) 2- (3-Dimethylaminophenyl) -4-ethyl-5- [6- methyl-6- ( 2H-tetrazol-5-yl) heptyloxyIphenol disodium salt;
PP) 3- (5- (6- ( 4-Pheny1-5-hydroxy-2 - ethylphenoxy) propoxy) -2-carboxymethyl- 1,2,3,4 -tetrahydronaphthalen-1 (2H) - one) propanoic acid; QQ) 3- (5- (6- (4- (4-Fluorophenyl) -5-hydroxy-2- ethylphenoxy) propoxy) -2-carboxymeth yl- 1,2,3,4-tetrahydronaphthalen-1 (2H) - one) propanoic acid;
RR) 3- (4- (5- (4- (4-Fluorophenyl) -5-hydroxy-2- ethylphenoxy) propoxy) -2-carboxymeth yl-2, 3- dihydroinden-1 (2H) -one) propanoic acid;
SS) 3, 3-Dimethyl-5- (3- (2-carboxyethyl) -4- (3- (4- fluorophenyl) -5-hydroxy-2- ethylphenoxy) propoxy) phenyl ) -5-oxopentanoic acid;
TT) 7-[3-[ (5-Ethyl-2-hydroxy[l,l '-biphenyl] -4- yl) oxy] propoxy] -3 , 4-dihydro-8-propyl-2H-l- benzopyran-2-carboxylic acid; UU) 8-Propyl-7- [3- [4- (4-fluorophenyl) -2-ethyl-5- hydroxyphenoxy] propoxy] -3 , 4-dihydro-2H-l- benzopyran-2-carboxylic acid;
W) 2- [3- [3- [ ( 5-Ethyl-2-hydroxy [1, 1 ' -biphenyl] - 4-yl) oxy] propoxy] -2-propylphenoxy] propanoic acid;
WW) 2- (4-Chloroρhenyl) -4-ethyl-5- [6-methyl-6- (2H-tetrazol-5-yl ) heptyloxy] phenol monosodium salt;
XX) 2- (3, 5-Dichlorophenyl) -4-ethyl-5- [ 6-methyl-
6- ( 2H-tetrazol-5-yl) heptyloxy] phenol monosodium salt;
YY) 3- [2- [3- [ ( 5-Ethyl-2-hydroxy [1, 1' -biphenyl] -
4-yl) oxy] propoxy] -1-dibenzofuran] propanoic acid disodium salt; ZZ) 7-Carboxy-9-oxo-3- [3- ( 2-ethyl-5-hydroxy-4- phenylphenoxy) propoxy] -9H-xanthene-4- propanoic acid disodium salt monohydrate;
AAA) 2- [2-Propyl-3- [3- ( 2-ethyl-5-hydroxy-4- phenylphenoxy) propoxy] phenoxy] benzoic acid sodium salt hemihydrate; BBB) 3 - [ 3 - ( 2 -Ethyl-5-hydroxy-4- phenylphenoxy) propoxy] [1,1' -biphenyl] -4- propanoic acid disodium salt monohydrate; CCC) 5-Ethyl-4-[3-[2-propyl-3-[2-(2H-tetrazol-5- yl ) phenoxy] phenoxy] propoxy] [1,1' -biphenyl ] - 2-ol disodium salt sesquihydrate;
DDD) 3-[4-[3-[ 3-(2-Ethyl-5-hydroxy-4- phenylphenoxy) propoxy] -9-oxo-9H- xanthene] ] propanoic acid sodium salt hemihydrate ;
EEE) 2-Fluoro-6- [2-propyl-3- [3- (2-ethyl-5- hydroxy-4- phenylphenoxy) propoxy] phenoxy] benzoic acid disodium salt;
FFF) 2- [2-Propyl-3- [3- [2-ethyl-4- (4- fluorophenyl ) -5- hydroxyphenoxy] propoxy] phenoxy] benzoic acid sodium salt;
GGG) 3- [4- [7-Carboxy-9-oxo-3- [3- [2-ethyl-4- (4- fluorophenyl) -5-hydroxyphenoxy] propoxy] -9H- xanthene] ] propanoic acid disodium salt trihydrate;
HHH) 3- [4- [9-OXO-3- [3- [2-ethyl-4- (4- fluorophenyl) -5-hydroxyphenoxy] propoxy] -9H- xanthene] ] propanoic acid;
III) 3-[2-[l-[2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy] propoxy] -4- (5-oxo-5- morpholinopentanamido) phenyl] propanoic acid;
JJJ) 2-Fluoro-6- [2-propyl-3- [3- [2-ethyl-5- hydroxy-4- ( 4- fluorophenyl ) phenoxy] propoxy] phenoxy] benzoic acid disodium salt hydrate;
KKK) 4-Fluoro-2- [2-propyl-3- [3- [2-ethyl-5- hydroxy-4- ( 4- fluorophenyl ) phenoxy] propoxy] phenoxy] benzoic acid; LLL) 2- [ 2 -Propyl-3 - [ 5- [ 2-ethyl-5-hydroxy-4- ( 4- fluorophenyl) phenoxy] pentoxy] phenoxy] benzoic acid; MMM) 2- [2-Propyl-3- [4- [2-ethyl-5-hydroxy-4- (4- fluorophenyl ) phenoxy] butoxy] phenoxy] benzoic acid sesquihydrate;
NNN) 2- [2- (2-Methylpropyl) -3- [3- [2-ethyl-5- hydroxy-4- ( 4- fluorophenyl ) phenoxy] propoxy] phenoxy] benzoic acid;
000) 2- [2-Butyl-3- [3- [2-ethyl-5-hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] phenoxy] benzoic acid hydrate;
PPP) 2- [2- (Phenylmethyl) -3- [3- [2-ethyl-5-hydroxy- 4-(4- fluorophenyl ) phenoxy] propoxy] phenoxy] benzoic acid;
QQQ) 2- [2-Propyl-3- [3- [2-ethyl-5-hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] phenoxy] phenyla cetic acid;
RRR) 2- [2-Propyl-3- [3- [2-ethyl-5-hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] benzoyl] benzoic acid;
SSS) 2- [ [2-Propyl-3- [3- [2-ethyl-5-hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] phenyl ] methyl ] b enzoic acid; TTT) 2-[2-Propyl-3-[3- [2-ethyl-4- (4- fluorophenyl) -5- hydroxyphenoxy] propoxy] thiophenoxy] benzoic acid; UUU) 2-[2-Propyl-3-[3-[2-ethyl-4-(4- fluorophenyl) -5- hydroxyphenoxy] propoxy] phenylsulfinyl ] benzoi c acid; VW) 2-[2-Propyl-3-[3-[2-ethyl-4-(4- fluorophenyl) -5- hydroxyphenoxy] propoxy] phenylsulfonyl ] benzoi c acid hydrate; WW) 5- [3- [2- (1-Carboxy) ethyl] -4- [3- [2-ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy] propoxy] phenyl ] -4-pentynoic acid disodium salt 0.4 hydrate;
XXX) 1-Phenyl-l- (lH-tetrazol-5-yl) -6- (2-ethyl-4- (4-fluorophenyl) -5-hydroxyphenoxy) hexane; YYY) 1- (4- (Carboxymethoxy) phenyl ) -1- ( IH-tetrazol-
5-yl) -6- (2-ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) hexane;
ZZZ) 1- (4- (Dimethylammocarbonylmethoxy) phenyl) - 1- (lH-tetrazol-5-yl) -6- (2-ethyl-4- (4- fluorophenyl ) -5-hydroxyphenoxy) hexane ;
AAAA) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) -E-propenoic acid;
BBBB) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl) -2-methyl-E- propenoic acid;
CCCC) 5- (2- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) phenyl ) ethyl ) -1H- tetrazole; DDDD) 3- (2- (3- (2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenoxy) propoxy) -4- (4- carboxybutyloxy) phenyl) propionic acid;
EEEE) 5- [3- [4- (4-Fluorophenyl ) -2-ethyl-5- hydroxyphenoxy] propoxy] -3, 4-dihydro-2H-l- benzopyran-2-one;
FFFF) 3- (3-{3- [2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenyloxy] propoxy}phenyl ) propanoic acid;
GGGG) 3- (3-{3- [2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenyloxy] propoxy} -4-propylph enyl) propanoic acid sodium salt;
HHHH) 3- (4-{3- [2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenyloxy] propoxy} -3- propylphenyl) propanoic acid; IIII) 3- (3-{3- [2-Ethyl-4- (4-fluorophenyl) -5- hydroxyphenyloxy] propoxy} -2- propylphenyl) propanoic acid;
JJJJ) 3-{3-[3-(2-Ethyl-5- hydroxyphenyloxy) propoxy] -2- propylphenyl}propanoic acid disodium salt; and
KKKK) 2- [3- [3- [2-Ethyl-5-hydroxy-4- (4- fluorophenyl ) phenoxy] propoxy] benzoyl ] benzoic acid disodium salt hemihydrate.
40. The method according to claim 36 wherein the leukotriene (LTB4) antagonist is a compound of the structure (Formula B) :
Formula B
namely, 2- [2-propyl-3- [3- [2-ethyl-5-hydroxy-4- (4- fluorophenyl) phenoxy] propoxy] phenoxy benzoic acid, and the pharmaceutically acceptable salts thereof.
41. The method according to claim 36 wherein the anti- cancer agent is selected from the group consisting of Busulfan, Carboplatin, Carmustine, Cisplatin, Cyclophosphamide, Dacarbazine, Ifosfamide, Lomustine, Streptozocin, Oxaliplatin, Temozolomide, Bleomycin, Dactinomycin, Daunorubicin, Doxorubicin, Idarubicin, Mitomycin-C, Plicamycin, Cryptophycin, Cytarabine, Floxuridine, Fludarabine, 5-Fluorouracil, Hydroxyurea, 6- Mercaptopurine, Methotrexate, Thioguanine; Capecitabine, Aldesleukin, Interferon Alfa-2A, Interleukin-2, Interleukin- 12 (recombinant) , Interferon Alfa-2B (recombinant) , Interferon Alfa-n3, Interferon Gamma-IB, Herceptin interleukin-2, interleukin-12, Aminoglutethimide,
Anastrozole, Flutamide, Goserelin, Leuprolide, Megestrol, Mitotane, Tamoxifen, Chlorambucil, Estramustine, Mechlorethamine, Melphalan, Thiotepa, Docetaxel, Etoposide, Irinotecan HCI, Paclitaxel, Teniposide, Topotecan, Vinblastine, Vincristine, Vinorelbine, Altretamine,
Amifostine Asparaginase-Esα erichia coli strain, BCG Live (Intravesical), Cladribine, Leucovorin, Levamisole, Mitoxantrone, Pegaspargase, Pentostatin, and Procarbazine.
42. The use of claim 40 wherein the anti-cancer agent is selected from the group consisting of Busulfan, Carboplatin, Carmustine, Cisplatin, Cyclophosphamide, Dacarbazine, Ifosfamide, Lomustine, Streptozocin, Oxaliplatin, Temozolomide, Bleomycin, Dactinomycin, Daunorubicin, Doxorubicin, Idarubicin, Mitomycin-C, Plicamycin, Cryptophycin, Cytarabine, Floxuridine, Fludarabine, 5-Fluorouracil, Hydroxyurea, 6-Mercaptopurine, Methotrexate, Thioguanine; Capecitabine, Aldesleukin, Interferon Alfa-2A, Interleukin-2, Interleukin-12 (recombinant) , Interferon Alfa-2B (recombinant) , Interferon Alfa-n3, Interferon Gamma-IB, Herceptin interleukin-2, interleukin-12, Aminoglutethimide, Anastrozole, Flutamide, Goserelin, Leuprolide, Megestrol, Mitotane, Tamoxifen, Chlorambucil, Estramustine, Mechlorethamine, Melphalan, Thiotepa, Docetaxel, Etoposide, Irinotecan HCI, Paclitaxel, Teniposide, Topotecan, Vinblastine, Vincristine, Vinorelbine, Altretamine, Amifostine Asparaginase- Escherichia coli strain, BCG Live (Intravesical) , Cladribine, Leucovorin, Levamisole, Mitoxantrone, Pegaspargase, Pentostatin, and Procarbazine.
EP00983695A 1999-11-11 2000-11-09 Oncolytic combinations for the treatment of cancer Withdrawn EP1231939A2 (en)

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Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU1916501A (en) * 1999-11-11 2001-06-06 Eli Lilly And Company Oncolytic combinations for the treatment of cancer
WO2001034198A2 (en) * 1999-11-11 2001-05-17 Eli Lilly And Company Oncolytic combinations for the treatment of cancer
JP2001247459A (en) 2000-03-03 2001-09-11 Oakland Uniservices Ltd Cancer combination therapy
CN1237968C (en) * 2000-05-09 2006-01-25 克赖顿大学 Method for inhibiting proliferation and inducing apoptosis of cancer cells
WO2002009700A1 (en) 2000-07-28 2002-02-07 Cancer Research Technology Limited Cancer treatment by combination therapy
GB0121285D0 (en) 2001-09-03 2001-10-24 Cancer Res Ventures Ltd Anti-cancer combinations
GB2386836B (en) 2002-03-22 2006-07-26 Cancer Res Ventures Ltd Anti-cancer combinations
GB2394658A (en) 2002-11-01 2004-05-05 Cancer Rec Tech Ltd Oral anti-cancer composition
WO2008044729A1 (en) 2006-10-12 2008-04-17 Institute Of Medicinal Molecular Design. Inc. Carboxylic acid derivative
US8633245B2 (en) 2008-04-11 2014-01-21 Institute Of Medicinal Molecular Design, Inc. PAI-1 inhibitor
ES2571452T3 (en) 2012-01-10 2016-05-25 Lilly Co Eli Leukotriene B4 antagonist compound

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES8602818A1 (en) * 1983-10-31 1985-12-01 Merck Frosst Canada Inc 5-Lipoxygenase inhibitors.
US4704400A (en) * 1986-06-24 1987-11-03 Merck & Co., Inc. Medicarpin derivatives and analogs
ES2062943B1 (en) * 1993-03-23 1995-11-16 Uriach & Cia Sa J NEW DERIVATIVES OF (2-METHYL-3-PIRIDIL) CYANOMETILPIPERAZINES.
US6034256A (en) * 1997-04-21 2000-03-07 G.D. Searle & Co. Substituted benzopyran derivatives for the treatment of inflammation

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO0134135A2 *

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