EP1194445A4 - Methods for protein purification using aqueous two-phase extraction - Google Patents
Methods for protein purification using aqueous two-phase extractionInfo
- Publication number
- EP1194445A4 EP1194445A4 EP00928866A EP00928866A EP1194445A4 EP 1194445 A4 EP1194445 A4 EP 1194445A4 EP 00928866 A EP00928866 A EP 00928866A EP 00928866 A EP00928866 A EP 00928866A EP 1194445 A4 EP1194445 A4 EP 1194445A4
- Authority
- EP
- European Patent Office
- Prior art keywords
- methods
- phase extraction
- aqueous
- protein purification
- growth hormone
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title abstract 3
- 238000000605 extraction Methods 0.000 title abstract 2
- 238000001742 protein purification Methods 0.000 title 1
- 229940122853 Growth hormone antagonist Drugs 0.000 abstract 1
- 102000002265 Human Growth Hormone Human genes 0.000 abstract 1
- 108010000521 Human Growth Hormone Proteins 0.000 abstract 1
- 239000000854 Human Growth Hormone Substances 0.000 abstract 1
- 238000002955 isolation Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Endocrinology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Biophysics (AREA)
- Gastroenterology & Hepatology (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/307,549 US6437101B1 (en) | 1999-05-07 | 1999-05-07 | Methods for protein purification using aqueous two-phase extraction |
US307549 | 1999-05-07 | ||
PCT/US2000/012320 WO2000068260A1 (en) | 1999-05-07 | 2000-05-04 | Methods for protein purification using aqueous two-phase extraction |
Publications (3)
Publication Number | Publication Date |
---|---|
EP1194445A1 EP1194445A1 (en) | 2002-04-10 |
EP1194445A4 true EP1194445A4 (en) | 2002-10-09 |
EP1194445B1 EP1194445B1 (en) | 2008-01-23 |
Family
ID=23190235
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP00928866A Expired - Lifetime EP1194445B1 (en) | 1999-05-07 | 2000-05-04 | Methods for purifying recombinant growth hormone antagonist proteins using aqueous two-phase extraction |
Country Status (13)
Country | Link |
---|---|
US (1) | US6437101B1 (en) |
EP (1) | EP1194445B1 (en) |
JP (1) | JP2002544139A (en) |
AT (1) | ATE384737T1 (en) |
AU (1) | AU777974B2 (en) |
BR (1) | BR0010707A (en) |
CA (1) | CA2372513A1 (en) |
DE (1) | DE60037886T2 (en) |
ES (1) | ES2303507T3 (en) |
IL (1) | IL146381A0 (en) |
MX (1) | MXPA01011356A (en) |
NZ (1) | NZ515381A (en) |
WO (1) | WO2000068260A1 (en) |
Families Citing this family (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6966992B2 (en) * | 2001-03-19 | 2005-11-22 | Akzo Nobel Nv | Method of purification of molecules using unbranched terminal alkyldiols |
CA2496687A1 (en) * | 2002-09-18 | 2004-04-01 | Centre Hospitalier De L'universite De Montreal (Chum) | Ghrh analogues |
DE502004005503D1 (en) * | 2004-06-25 | 2007-12-27 | Kuehni Ag | Method for extraction of biological material |
DE602005009827D1 (en) * | 2004-06-29 | 2008-10-30 | Ares Trading Sa | PROCESS FOR PURIFYING IL-18 BINDING PROTEIN |
US20070048786A1 (en) * | 2005-09-01 | 2007-03-01 | Arnon Chait | Systems and methods for fractionation of protein mixtures |
US20080070230A1 (en) * | 2006-06-12 | 2008-03-20 | Wyeth | Methods for reducing or preventing liquid-liquid phase separation in high concentration protein solutions |
KR100836171B1 (en) * | 2007-01-19 | 2008-06-09 | 인하대학교 산학협력단 | Affinity separation by partition enhancing material conjugated biomolecules in aqueous two-phase extraction system |
DE102008013490A1 (en) | 2008-03-10 | 2009-09-17 | Bayer Technology Services Gmbh | Process for the purification of therapeutic proteins |
CN102224160A (en) * | 2008-11-25 | 2011-10-19 | 通用电气健康护理生物科学股份公司 | Aqueous two phase extraction augmented precipitation process for purification of therapeutic proteins |
DE102011001743A1 (en) * | 2011-04-01 | 2012-10-04 | Technische Universität Dortmund | Process for separation / purification of biomolecules |
CN102662015B (en) * | 2012-04-12 | 2013-11-20 | 湖南师范大学 | Biological sample purification method based on inorganic salt promoted phase transition and separation of nano-gold |
US20130310554A1 (en) * | 2012-05-18 | 2013-11-21 | Hong Kong Baptist University | Aqueous Diphase Solvent System for Preparing High-Purity Polysaccharides |
CN111249531B (en) * | 2020-01-19 | 2022-03-22 | 巴斯特医药科技(常州)有限公司 | Method for preparing collagen scaffold by using high-activity plant enzyme |
CN114957494A (en) * | 2021-02-23 | 2022-08-30 | 江苏农牧科技职业学院 | Process for extracting protein in polygonatum polysaccharide by adopting double aqueous phases |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5815989A (en) * | 1981-06-12 | 1983-01-29 | Sanki Eng Kk | Purification of physiologically active protein and its device |
EP0387760A1 (en) * | 1989-03-15 | 1990-09-19 | Forschungszentrum Jülich Gmbh | Process to extract proteins from aqueous biomass suspensions |
WO1992007868A1 (en) * | 1990-11-06 | 1992-05-14 | Kabi Pharmacia Ab | A method for isolating and purifying peptides and proteins |
US5734024A (en) * | 1996-04-19 | 1998-03-31 | Boris Y. Zaslavsky | Method for determining the biological activity of recombinant human growth hormone |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DK144731C (en) | 1976-07-30 | 1982-10-18 | Nordisk Insulinlab | PROCEDURE FOR INSULATING PROTEIN HORMONES DERIVED FROM HUMAN HYPPHYPHYSE TISSUE |
US5139943A (en) * | 1989-06-13 | 1992-08-18 | Genencor International, Inc. | Processes for the recovery of microbially produced chymosin |
US5151358A (en) | 1989-06-13 | 1992-09-29 | Genencor International, Inc. | Processes for the recovery of naturally produced chymosin |
US5182369A (en) | 1990-02-28 | 1993-01-26 | Monsanto Company | Method for purifying somatotropin monomers |
US5328841A (en) * | 1990-10-05 | 1994-07-12 | Genencor International, Inc. | Methods for isolating EG III cellulase component and EG III cellulase in polyethylene glycol using inorganic salt and polyethylene glycol |
US5407810A (en) | 1993-08-20 | 1995-04-18 | Genentech, Inc. | Aqueous multiple-phase isolation of polypeptide |
GB0205898D0 (en) * | 2002-03-13 | 2002-04-24 | Pharmacia Ab | Treatment of type I diabetes mellitus |
-
1999
- 1999-05-07 US US09/307,549 patent/US6437101B1/en not_active Expired - Lifetime
-
2000
- 2000-05-04 JP JP2000616234A patent/JP2002544139A/en not_active Withdrawn
- 2000-05-04 BR BR0010707-7A patent/BR0010707A/en not_active IP Right Cessation
- 2000-05-04 WO PCT/US2000/012320 patent/WO2000068260A1/en active Search and Examination
- 2000-05-04 ES ES00928866T patent/ES2303507T3/en not_active Expired - Lifetime
- 2000-05-04 EP EP00928866A patent/EP1194445B1/en not_active Expired - Lifetime
- 2000-05-04 NZ NZ515381A patent/NZ515381A/en unknown
- 2000-05-04 AU AU47036/00A patent/AU777974B2/en not_active Ceased
- 2000-05-04 AT AT00928866T patent/ATE384737T1/en not_active IP Right Cessation
- 2000-05-04 DE DE60037886T patent/DE60037886T2/en not_active Expired - Fee Related
- 2000-05-04 MX MXPA01011356A patent/MXPA01011356A/en active IP Right Grant
- 2000-05-04 IL IL14638100A patent/IL146381A0/en unknown
- 2000-05-04 CA CA002372513A patent/CA2372513A1/en not_active Abandoned
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5815989A (en) * | 1981-06-12 | 1983-01-29 | Sanki Eng Kk | Purification of physiologically active protein and its device |
EP0387760A1 (en) * | 1989-03-15 | 1990-09-19 | Forschungszentrum Jülich Gmbh | Process to extract proteins from aqueous biomass suspensions |
WO1992007868A1 (en) * | 1990-11-06 | 1992-05-14 | Kabi Pharmacia Ab | A method for isolating and purifying peptides and proteins |
US5734024A (en) * | 1996-04-19 | 1998-03-31 | Boris Y. Zaslavsky | Method for determining the biological activity of recombinant human growth hormone |
Non-Patent Citations (3)
Title |
---|
DATABASE CONFERENCE PAPERS INDEX 12 October 1999 (1999-10-12), VALAX,P.: ""Primary purification step: Aqueous two phase liquid-liquid extraction of a modified growth hormone from homogenized E. coli"", XP002204007 * |
DATAR R ET AL: "STUDIES ON THE REMOVAL OF ESCHERICHIA-COLI CELL DEBRIS BY AQUEOUS TWO-PHASE POLYMER EXTRACTION", JOURNAL OF BIOTECHNOLOGY, vol. 3, no. 4, 1986, pages 207 - 220, XP001084800, ISSN: 0168-1656 * |
DYR J E ET AL: "Separation used for purification of recombinant proteins", JOURNAL OF CHROMATOGRAPHY B: BIOMEDICAL SCIENCES & APPLICATIONS, ELSEVIER SCIENCE PUBLISHERS, NL, vol. 699, no. 1-2, 10 October 1997 (1997-10-10), pages 383 - 401, XP004095005, ISSN: 1570-0232 * |
Also Published As
Publication number | Publication date |
---|---|
MXPA01011356A (en) | 2003-08-20 |
WO2000068260A1 (en) | 2000-11-16 |
DE60037886D1 (en) | 2008-03-13 |
IL146381A0 (en) | 2002-07-25 |
ATE384737T1 (en) | 2008-02-15 |
AU777974B2 (en) | 2004-11-04 |
US6437101B1 (en) | 2002-08-20 |
BR0010707A (en) | 2002-06-04 |
EP1194445B1 (en) | 2008-01-23 |
ES2303507T3 (en) | 2008-08-16 |
AU4703600A (en) | 2000-11-21 |
JP2002544139A (en) | 2002-12-24 |
CA2372513A1 (en) | 2000-11-16 |
EP1194445A1 (en) | 2002-04-10 |
NZ515381A (en) | 2003-10-31 |
DE60037886T2 (en) | 2009-01-22 |
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Inventor name: HAYENGA, KIRK, J. Inventor name: VALEX, PASCAL, P. |
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