EP1180111A4 - Methode et appareil de preparation d'une banque de composes utilisant un modulateur optique - Google Patents

Methode et appareil de preparation d'une banque de composes utilisant un modulateur optique

Info

Publication number
EP1180111A4
EP1180111A4 EP00904105A EP00904105A EP1180111A4 EP 1180111 A4 EP1180111 A4 EP 1180111A4 EP 00904105 A EP00904105 A EP 00904105A EP 00904105 A EP00904105 A EP 00904105A EP 1180111 A4 EP1180111 A4 EP 1180111A4
Authority
EP
European Patent Office
Prior art keywords
micro
compound
pluralities
wells
light
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP00904105A
Other languages
German (de)
English (en)
Other versions
EP1180111A1 (fr
Inventor
Jeong-Sun Seo
Yoon-Sik Lee
Yong-Kweon Kim
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Macrogen Inc
Original Assignee
Macrogen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Macrogen Inc filed Critical Macrogen Inc
Publication of EP1180111A1 publication Critical patent/EP1180111A1/fr
Publication of EP1180111A4 publication Critical patent/EP1180111A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B01J2219/00315—Microtiter plates
    • B01J2219/00317—Microwell devices, i.e. having large numbers of wells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00436—Maskless processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00436—Maskless processes
    • B01J2219/00439—Maskless processes using micromirror arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00527—Sheets
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00527—Sheets
    • B01J2219/00529—DNA chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/0059—Sequential processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00612—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports the surface being inorganic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00614—Delimitation of the attachment areas
    • B01J2219/00617—Delimitation of the attachment areas by chemical means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00614—Delimitation of the attachment areas
    • B01J2219/00621—Delimitation of the attachment areas by physical means, e.g. trenches, raised areas
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00623—Immobilisation or binding
    • B01J2219/00626—Covalent
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00632—Introduction of reactive groups to the surface
    • B01J2219/00637—Introduction of reactive groups to the surface by coating it with another layer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00686—Automatic
    • B01J2219/00689—Automatic using computers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00709—Type of synthesis
    • B01J2219/00711—Light-directed synthesis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
    • C07B2200/00—Indexing scheme relating to specific properties of organic compounds
    • C07B2200/11—Compounds covalently bound to a solid support
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries

Definitions

  • the present invention relates to a providing of a compound library, more particularly to a providing of a compound library preparation method using an optical modulator and a compound library preparation apparatus. Furthermore, the present invention provides a compound analyzing method
  • desired compounds are synthesized are bonded to the functional groups, and then light is irradiated only on the necessary area thereof so that the functional groups are exposed.
  • a compound library is synthesized.
  • the present invention provides a method and apparatus for effectively preparing varieties of compounds in a simple process without
  • the present invention seeks to decrease error occurrence that is
  • the present invention easily provides various compound libraries by
  • the present invention provides a method for the preparation of a
  • optical modulator is of a construction in which pluralities of
  • optical switches are aligned, and each optical switch is a device
  • micro-well or moves or rotates an opaque masking plate to
  • step b) can be substituted with a step
  • the method of the present invention can be applied to a variety of
  • the present invention provides compound library chips
  • the present invention provides an apparatus for the preparation of
  • micro-wells that are not to be bonded; and e) a controller comprising at least one optical switch address, irradiating light on a micro-well according to the time, order of compounds to be injected, and information on final compounds produced on each micro-well, and having programs controlling the operating order of the optical irradiator, the compound injecting device, and the washing device.
  • Typical configurations of an optical modulator include the following
  • the first example is an optical modulator having a separate medium
  • a substrate plate which supports the micro-mirror, aligned on a plane, placed separately from the micro-mirror, and comprising
  • each micro-mirror, a landing electrode, and the micro-mirror is a micro-mirror, a landing electrode, and the micro-mirror
  • optical modulator comprising:
  • Another example is an optical modulator irradiating light only at the
  • desired micro-wells comprising:
  • the present invention provides a compound analyzing
  • the present invention provides a compound analyzing
  • FIG. 1 apparatus (FIG. 1 ) comprising:
  • Exemplary compounds of the present invention include varieties of
  • wall as used in the present invention not only refers to the meaning usually used in the field of the present invention but also as a
  • FIG. 2 consists of pluralities of micro-mirrors (a) on a substrate plate (b) (FIG. 2),
  • micro-mirrors are individually adjusted with electrical signals.
  • Each micro-mirror can irradiate light only on a particular micro-well of the substrate plate, which comprises corresponding pluralities of micro-wells of the same magnitude, so that functional groups are activated only at the desired position. Furthermore, other libraries can be obtained depending on the quantity of light irradiated since even the same method can control the reflecting time of a micro-mirror.
  • the present invention is based on an idea that various types of biochips can be effectively manufactured if the micro-mirror is used instead of a photomask as in the previous compound
  • An optical modulator can two-dimensionally align a micro-mirror (a)
  • Each micro-mirror has rotation angles of at least
  • FIG. 4 a illustrates a micro-well
  • micro-mirror c illustrates the incident light
  • d illustrates the reflected light
  • optical modulator The embodiment examples of an optical modulator are as follows.
  • micro-mirror a is connected to pillar c by torsion spring b, wherein pillar c is fixed to substrate plate d as shown in FIG.
  • Micro-mirror a is tilted by applying voltage to one side of electrode e,
  • micro-mirror b is manufactured
  • micro-mirror b can be driven by cladding piezoelectric material or bimetal over cantilever a, as shown in FIG. 6.
  • a is a micro-mirror
  • b is an address electrode
  • c is a landing electrode
  • d is a hinge supporting post
  • e is an electrode supporting post.
  • f1 and f2 illustrate the states of tilt when a voltage is applied to an electrode.
  • Korean Patent Application No. 96-20611 can be referred to as the fourth embodiment example.
  • a prescribed patterned electrode (b) is formed
  • the electrode (b) has a strip shape
  • a post (f) is formed on the substrate
  • a micro-mirror is rotationally connected to the
  • a prescribed perforative slot (g) is formed on the top surface of a micro-mirror, and the post-integrated perforative slot is formed.
  • light can be irradiated through a
  • FIG. 9 and FIG. 10 is a state in which light of two windows is masked, and (b) is a state in which light of the right side windows is irradiated.
  • a protecting group of the present invention means a material that is
  • protecting groups include ⁇ -methyl-2-
  • Examples of an activator of the present invention preferably include
  • the controller program is an important component in the apparatus
  • This program should at least include: the types of compounds injected;
  • optical switch through a code which identifies each optical switch; the time of
  • additional apparatus includes a circuit that deciphers a controller code.
  • the prepared compound libraries are used to measure the activity of
  • the measuring example comprises the steps of i )
  • FIG. 1 illustrates a compound library preparation apparatus and a
  • FIG. 2 is an optical modulator in which pluralities of micro-mirrors
  • optical switch are configured by an optical switch
  • FIG. 3 illustrates a state in which light is reflected on a micro-well
  • FIG. 4 illustrates a state in which a micro-mirror does not reflect light
  • FIG. 5 to FIG. 10 are embodiment examples of an optical modulator
  • FIG. 1 1 (a) and FIG. 1 1 (b) are front and side scanning electron
  • FIG. 12 illustrates a direction in which a mirror array pixel bloc is
  • FIG. 13 is a microscope photograph in which a practically driven
  • FIG. 14 illustrates a procedure in which polynucleotide library is
  • FIG. 15 illustrates a procedure in which a polypeptide library is
  • the invention is capable of modification in
  • FIG. 12 illustrates a direction in which a mirror array pixel bloc is
  • FIG. 13 is a microscope photograph in which the practically
  • the floor of the micro-well was washed with the chloroform solution and dried.
  • reaction termination can be confirmed when the color is removed.
  • nucleotide used in the reaction can be easily combined with an amino group on a chip due to its carboxylic acid shape, and the 5' position has a
  • Ultraviolet rays of 360 nm were irradiated through a micro-mirror for 15 minutes, removing a protecting group at a desired position. After washing
  • nucleotide used in the reaction can be easily combined with the 5' position
  • nucleotide has a protecting group which is decomposed by light (Chemical Formula 4).
  • FIG. 14 represents a process in which
  • polynucleotide C-G-T-A and A-G-T-C are prepared through the above
  • a process for synthesizing a peptide library on a chip is similar to the foregoing.
  • a compound library chip employing a protecting group at a terminal end amine group was mounted on a system and washed with dried acetonitrile.
  • a protecting group was selectively removed and washed with a dried NMP solution. It was reacted for 60 minutes by injecting the already prepared 0.1 M amino acid/ NMP solution and 0.5 M BOP/ HOBt/NMP solution into a micro-well. After the coupling reaction, it was washed with the NMP solution for 10 minutes.
  • Each amino acid used in the coupling reaction has a protecting group of
  • FIG. 15 represents a process in which
  • polypeptide G-E-C-A and H-F-D-B are synthesized through the above
  • the present invention has the following merits because of the use of an optical modulator instead of conventionally used photomasks.
  • an optical switch can be individually operated.
  • an automation of the compound library preparation process can be more easily accomplished since the optical irradiation position and quantity of light can be simply controlled by an electrical signal of a controller. Furthermore, it is appropriate to compose only the program itself even though other configurations are required since the compound library
  • compound composition can be easily modified by controlling the quantity of

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Nanotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Composite Materials (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • General Physics & Mathematics (AREA)
  • Materials Engineering (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)

Abstract

La présente invention concerne la fourniture de banques de composés, plus particulièrement elle a trait une méthode de préparation de banques de composés utilisant un modulateur optique ainsi qu'un appareil de préparation de banques de composés utilisé dans ladite méthode. En outre, la présente invention concerne une méthode d'analyse de composés utilisant la méthode de préparation ainsi qu'un appareil d'analyse de composés utilisé dans celle-ci.
EP00904105A 1999-02-10 2000-02-10 Methode et appareil de preparation d'une banque de composes utilisant un modulateur optique Withdrawn EP1180111A4 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR19990004639 1999-02-10
KR9904639 1999-02-10
PCT/KR2000/000100 WO2000047598A1 (fr) 1999-02-10 2000-02-10 Methode et appareil de preparation d'une banque de composes utilisant un modulateur optique

Publications (2)

Publication Number Publication Date
EP1180111A1 EP1180111A1 (fr) 2002-02-20
EP1180111A4 true EP1180111A4 (fr) 2003-03-26

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EP00904105A Withdrawn EP1180111A4 (fr) 1999-02-10 2000-02-10 Methode et appareil de preparation d'une banque de composes utilisant un modulateur optique

Country Status (4)

Country Link
EP (1) EP1180111A4 (fr)
JP (1) JP2002541433A (fr)
AU (1) AU2577700A (fr)
WO (1) WO2000047598A1 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2332645A1 (fr) 1998-02-11 2011-06-15 The University Of Houston Appareil pour des réactions chimiques et biochimiques au moyen de réactifs photogénérés
US6271957B1 (en) * 1998-05-29 2001-08-07 Affymetrix, Inc. Methods involving direct write optical lithography
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