EP1165507A1 - Biocatalyst and its use in enzymatic resolution of racemic beta-lactams - Google Patents
Biocatalyst and its use in enzymatic resolution of racemic beta-lactamsInfo
- Publication number
- EP1165507A1 EP1165507A1 EP00918984A EP00918984A EP1165507A1 EP 1165507 A1 EP1165507 A1 EP 1165507A1 EP 00918984 A EP00918984 A EP 00918984A EP 00918984 A EP00918984 A EP 00918984A EP 1165507 A1 EP1165507 A1 EP 1165507A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- lactam
- process according
- enantiomerically enriched
- ncimb
- accession
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D205/00—Heterocyclic compounds containing four-membered rings with one nitrogen atom as the only ring hetero atom
- C07D205/12—Heterocyclic compounds containing four-membered rings with one nitrogen atom as the only ring hetero atom condensed with carbocyclic rings or ring systems
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C12N9/86—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in cyclic amides, e.g. penicillinase (3.5.2)
Definitions
- This invention relates to a process for the production of optically active ⁇ -lactams by enzymatic resolution of the racemic ⁇ -lactam.
- ⁇ -lactam ring as a structural feature in biologically important compounds is well established, as is the synthetic utility of ⁇ -lactams.
- the utility of ⁇ -lactams as masked ⁇ -amino acids is illustrated by their use as precursors to the C-13 taxol side chain.
- the active molecules are often chiral, and it is preferable that they are accessible in the form of single enantiomers. This feature is also utilised in the design of stereodefined scaffolds, to generate single enantiomer compounds libraries for initial lead identification as part of a drug discovery programme.
- Alicyclic ⁇ -amino acids can be cyclised to the corresponding ⁇ -lactams, typically by use of carbod ⁇ mide reagents. They are also compounds having biological or pharmaceutical activity.
- the natural product cispentacin, (-)-(lR,2S)-2- aminocyclopentanecarboxylic acid has potent antijfungal properties. It is synthesised as a natural product in Streptomyces setonii and Bacillus cereus, and is inhibitory to strains of Candida.
- the same amino acid has also been used to probe the relationship between structure and taste in L-aspartyl dipeptides, where the absolute structure of the ⁇ -amino acid strongly affected the taste of the dipeptide.
- Optically pure alicyclic ⁇ -amino acids may be made, for example, by the enzyme- catalysed solvent-based bioresolution of the amino carboxyl esters (Kanerva et al. Tetrahedron: Asymmetry, 1996, 7, 1705).
- Such racemic amino esters are readily synthesised from ⁇ -lactams of formula (1), which are themselves synthesised by cycloaddition of the appropriate cyclic alkene and chlorosulfonyl isocyanate, followed by reductive work-up using sodium sulfite:
- WO-A-97/10713 and EP-A-0232017 disclose various bicyclic compounds, including 7-azabicyclo[4.2.0]oct-4-en-8-one of the formula
- WO-A-97/10713 includes reference to enantiomers, but there is no evident means of resolution. For example, the compounds will not readily form salts with chiral resolving agents.
- EP-A-0002564 discloses ⁇ -lactams, including bicyciic structures. It is suggested that the racemic lactams might be resolved, and refers to GB-A- 1273278, but that depends on melt crystallisation, and probably requires that the substrate exists as a conglomerate.
- the present invention is based on the discovery of a novel lactamase biocatalyst that allows efficient access to a range of synthetically useful ⁇ -lactams and the corresponding ⁇ -amino acids.
- a preferred embodiment of the invention is the application of this methodology to the preparation of novel single enantiomer cyclohexene-fused ⁇ - lactams.
- the novel biocatalyst is capable of enantioselective hydrolysis of racemic lactams of the formulae (2) and (3)
- a further aspect of the invention lies in the novel, enantiomerically enriched compounds of formulae (2) and (3), preferably as the levorotatory enantiomer.
- Lactams (2) and (3) are conveniently synthesised by cycloaddition of chlorosulfonyl isocyanate with norbornadiene and cyclohexa-l,3-diene respectively (Stajer et al, Tetrahedron, 1984, 40, 2385; Malpass et al, J. Chem. Soc, Perkin Trans. 1, 1991, 2276).
- Enantiomerically enriched products obtained from the bioresolution of lactams (2) and (3) can be used to prepare information-rich chiral scaffolds for elaboration into single enantiomer compounds libraries.
- Thealkene functionality is amenable to a range of transformations, especially through reaction with oxidants.
- conversion of (3) to the chiral scaffold (4) gives three points for structural elaboration into defined regions of 3-dimensional space.
- the more rigid scaffold (5) can be prepared via bioresolution of (2).
- the present invention embodies a novel ⁇ -lactamase biocatalyst from Pseudomonas putida, combining the key attributes of high enantioselectivity with superior catalytic activity to ⁇ -lactamases described previously.
- This biocatalyst has been deposited (see Example 1). Use of this biocatalyst allows certain single enantiomer ⁇ - lactams to be prepared for the first time in synthetically useful amounts.
- the biocatalyst can be used under conditions that can readily be determined, to produce a mixture of compounds. These can be separated by known methods. More particularly, the enantiomerically enriched ⁇ -amino acid produced by hydrolysis can be isolated. The isolated ⁇ -amino acid may then be subjected to a condensation reaction, to reform the ⁇ -lactam ring.
- the biocatalyst can be used in whole cell or paste form.
- the enzyme can also be isolated, by techniques known to those skilled in the art.
- Examples 1 , 2, 4, 6 and 8 illustrate the invention.
- Examples 3 , 5 and 7 illustrate the preparation of racemic substrates.
- TFAA is trifluoroacetic anhydride.
- Glycerol stocks of 96 bacterial strains were used to inoculate 1.0ml Tryptone soya broth (Oxoid CM 129) per well in 2.2ml 96-well plates (Advanced Biotechnologies AB-0661). These were then shaken at 25°C on a Heidolph Titramax 1000 incubator at max rpm for 45 hours. The cells were harvested by centrifugation at 1 OOOg, 4°C, for 10 minutes, and the cell pastes were stored at -20°C.
- a lO ⁇ l loopfiil of colony of CMC 103381 was used to inoculate seed flasks (100ml Tryptone soya broth (Oxoid CM 129) per 500ml Erlenmeyer flask). These were shaken at 25°C, 3 OOrpm in a temperature-controlled shaker (New Brunswick
- the fermentation medium contained, per litre: 15g yeast extract (Oxoid L21), 8g KH 2 PO 4 , 7g K 2 HPO , lg MgSO 4 .7H 2 O, lg (NH 4 ) 2 SO 4 , 1ml trace elements solution, 1.0ml polypropylene glycol (Merck 29767 6Y), and 20g glucose.
- the trace elements solution contained, per litre: 250ml cone.
- the 15ml of buffered solution remaining from the bioresolution described above contain lg (7.1 mmol) of amino acid (maximum).
- To the stirred buffer solution was slowly added, at IOC, 1.55g (7.1mmol) dibutyl dicarbonate dissolved in 20ml THF.
- the pH of the solution was maintained at 9 by addition of 3M NaOH solution.
- the reaction mixture was allowed to warm up to room temperature and left stirring overnight. The reaction was halted after 18 hours and THF was removed by rotary evaporator.
- the aqueous reaction mixture was acidified to pH3 with 10% potassium hydrogen sulfate solution.
- the mixture was then extracted with 3 x 50ml of ethyl acetate and the combined organic extracts were dried over magnesium sulfate. Removal of solvent by rotary evaporator yielded 1.1 g, 62% of a white solid.
- Cyclopentadiene was freshly prepared by thermolysis of cyclopentadiene dimer.
Abstract
A process for the preparation of an enantiomerically enriched β-lactam, comprises enantioselective hydrolysis of the corresponding racemic β-lactam in the presence of a lactamase enzyme capable of enantioselective hydrolysis of 3-azatricyclo[4.2.1.02,5]non-7-en-4-one and 7-azabicyclo[4.2.0]oct-4-en-8-one.
Description
BIOCATALYST AND ITS USE IN ENZYMATIC RESOLUTION OF RACEMIC
BETA-LACTAMS
Field of the Invention
This invention relates to a process for the production of optically active β-lactams by enzymatic resolution of the racemic β-lactam. Background of the Invention
The utility of the β-lactam ring as a structural feature in biologically important compounds is well established, as is the synthetic utility of β-lactams. For example, the utility of β-lactams as masked β-amino acids is illustrated by their use as precursors to the C-13 taxol side chain. The active molecules are often chiral, and it is preferable that they are accessible in the form of single enantiomers. This feature is also utilised in the design of stereodefined scaffolds, to generate single enantiomer compounds libraries for initial lead identification as part of a drug discovery programme.
Alicyclic β-amino acids can be cyclised to the corresponding β-lactams, typically by use of carbodϋmide reagents. They are also compounds having biological or pharmaceutical activity. For example, the natural product cispentacin, (-)-(lR,2S)-2- aminocyclopentanecarboxylic acid, has potent antijfungal properties. It is synthesised as a natural product in Streptomyces setonii and Bacillus cereus, and is inhibitory to strains of Candida. The same amino acid has also been used to probe the relationship between structure and taste in L-aspartyl dipeptides, where the absolute structure of the β-amino acid strongly affected the taste of the dipeptide.
Optically pure alicyclic β-amino acids may be made, for example, by the enzyme- catalysed solvent-based bioresolution of the amino carboxyl esters (Kanerva et al. Tetrahedron: Asymmetry, 1996, 7, 1705). Such racemic amino esters are readily synthesised from β-lactams of formula (1), which are themselves synthesised by cycloaddition of the appropriate cyclic alkene and chlorosulfonyl isocyanate, followed by reductive work-up using sodium sulfite:
It is generally preferable to resolve enantiomers as early as possible in a synthetic sequence, to avoid having to perform the chemistry on the racemate at twice the scale as for the enantiomer. Thus, it is useful to be able to access β-lactam precursors of β-amino acids in resolved form. One approach is to derivatise the β-lactam to theN-hydroxymethyl- β-lactam using paraformaldehyde (Csomόs et al, Tetrahedron: Asymmetry, 1996, 7, 1789). The resulting primary alcohols can then be resolved using a lipase, for example to access the O-acylated precursor to cispentacin, as depicted below. Besides the extra derivatisation and deprotection required, the resolution with lipase AK requires high enzyme loading and chromatographic separation of the products, making this general approach unattractive at scale.
WO-A-97/10713 and EP-A-0232017 disclose various bicyclic compounds, including 7-azabicyclo[4.2.0]oct-4-en-8-one of the formula
(3)
These compounds are in racemic form. WO-A-97/10713 includes reference to enantiomers, but there is no evident means of resolution. For example, the compounds will not readily form salts with chiral resolving agents.
EP-A-0002564 discloses β-lactams, including bicyciic structures. It is suggested that the racemic lactams might be resolved, and refers to GB-A- 1273278, but that depends on melt crystallisation, and probably requires that the substrate exists as a conglomerate.
Kurihara et al, Tetrahedron Lett., 1985, 26 (47), 5831, and also Tamara et al, Tetrahderon Lett. 1986, 27 (32), 3749, disclose a β-lactam, in single enantiomer form, of the formula
In the former paper, enzymatic desymmetrisation of a precursor (before conversion to a β-amino acid and then formation of the lactam ring) is involved. The latter involves classical resolution, using cinchonidine, of a ring-open precursor.
A more direct approach would be to resolve the lactam itself, thereby removing the separation problem evident in the work of Csomόs et al (reference cited above), since the resulting amino acid is soluble in buffer, whilst the unreacted substrate can be extracted into solvent. This has been shown in one instance (Evans et al, J. Chem. Soc, Perkin Trans. 1, 1991, 2276; see also WO-A-92/ 18477) where Rhodococcus equi was used to provide (1R, 5S)-6-azabicyclo[3.2.0]hept-3-ene-7-one:
However, this biotransformation is an exceedingly slow process and is therefore not viable for operation on a commercial scale; 980mg whole-cell paste was needed to resolve 340mg racemate in 212 hrs, in order to recover unreacted lactam of >99% ee. Summary of the Invention
The present invention is based on the discovery of a novel lactamase biocatalyst that allows efficient access to a range of synthetically useful β-lactams and the corresponding β-amino acids. A preferred embodiment of the invention is the application of this methodology to the preparation of novel single enantiomer cyclohexene-fused β- lactams. More particularly, the novel biocatalyst is capable of enantioselective hydrolysis of racemic lactams of the formulae (2) and (3)
(2) (3)
A further aspect of the invention lies in the novel, enantiomerically enriched compounds of formulae (2) and (3), preferably as the levorotatory enantiomer. Description of the Invention
For identification of a suitable biocatalyst, a microbial screen was targeted at hydrolysis of racemic lactams (2) and (3). Hitherto, neither compound has been prepared in enantiomerically enriched form. Lactams (2) and (3) are conveniently synthesised by cycloaddition of chlorosulfonyl isocyanate with norbornadiene and cyclohexa-l,3-diene respectively (Stajer et al, Tetrahedron, 1984, 40, 2385; Malpass et al, J. Chem. Soc, Perkin Trans. 1, 1991, 2276).
400 Microbial strains were screened for hydrolysis of the lactams (2) and (3). Surprisingly, one strain of Pseudomonas putida not only hydrolysed the lactam, but did so selectively, with much higher activity than observed for the prior art strain of Rhodococcus (Evans et al, reference as above). Thus, for lactam (2), when stirred with an equal weight of cell paste in phosphate buffer at 25°C, resolution was complete after 15 hours (>98% ee residual substrate), and the enantiomerically pure lactam extracted into
ethyl acetate in 38% yield. Similarly for lactam (3), after incubation with an equal weight of cells for 21 hours in buffer, the ee of residual lactam was >90%. Recovery of the enantiomerically enriched β-amino acids from aqueous solution is facilitated by conversion to N-Boc derivatives under standard conditions, followed by solvent extraction.
Enantiomerically enriched products obtained from the bioresolution of lactams (2) and (3) can be used to prepare information-rich chiral scaffolds for elaboration into single enantiomer compounds libraries. Thealkene functionality is amenable to a range of transformations, especially through reaction with oxidants. For example, conversion of (3) to the chiral scaffold (4) gives three points for structural elaboration into defined regions of 3-dimensional space. Similarly, the more rigid scaffold (5) can be prepared via bioresolution of (2).
(3) (4)
(2) (5)
In summary, the present invention embodies a novel β-lactamase biocatalyst from Pseudomonas putida, combining the key attributes of high enantioselectivity with superior catalytic activity to β-lactamases described previously. This biocatalyst has been deposited (see Example 1). Use of this biocatalyst allows certain single enantiomer β- lactams to be prepared for the first time in synthetically useful amounts.
As will be evident to those skilled in the art, the biocatalyst can be used under conditions that can readily be determined, to produce a mixture of compounds. These can be separated by known methods. More particularly, the enantiomerically enriched β-amino acid produced by hydrolysis can be isolated. The isolated β-amino acid may then be subjected to a condensation reaction, to reform the β-lactam ring.
The biocatalyst can be used in whole cell or paste form. The enzyme can also be isolated, by techniques known to those skilled in the art.
The following Examples 1 , 2, 4, 6 and 8 illustrate the invention. Examples 3 , 5 and 7 illustrate the preparation of racemic substrates. TFAA is trifluoroacetic anhydride. Example 1 Biocatalyst
Glycerol stocks of 96 bacterial strains (obtained from the Applicant's strain collection) were used to inoculate 1.0ml Tryptone soya broth (Oxoid CM 129) per well in 2.2ml 96-well plates (Advanced Biotechnologies AB-0661). These were then shaken at 25°C on a Heidolph Titramax 1000 incubator at max rpm for 45 hours. The cells were harvested by centrifugation at 1 OOOg, 4°C, for 10 minutes, and the cell pastes were stored at -20°C.
Cell pastes of the 96-well culture plates SCL0003 were resuspended in 0.5ml of
20g.L-' of substrate in 0.1M KH2PO4, pH 7.0 (13.6g KH2PO4 in l.OL water, adjusted to pH 7.0 with 12M NaOH) and then shaken at 25°C on a Heidolph Titramax 1000 incubator at max rpm for 40 (on the norbornadiene lactam (2) derivative) to 66 hours (on the cyclohexa-l,3-diene lactam (3) derivative). Reactions were stopped by diluting 1 in 10 in a 1 : 1 mix of MeOH: 1 OmM KH2PO , pH 7.0 ( 1.36g KH2PO4 in 1.0L water, adjusted to pH
7.0 with 12M NaOH). These were then assayed by HPLC. A 15cm 5μ Hichrom KR100
C8 Column was used with a running buffer of a 1 : 1 mix of MeOH: 1 OmM KH2PO4, pH 7.0 (1.36g KH2PO4 in l.OL water, adjusted to pH 7.0 with 12M NaOH) at a flow rate of l.Oml.min'1. Detection was at 210 nm. Results showed that a strain of Pseudomonas putida had reached 45% conversion on the norbornadiene lactam derivative and 43% conversion on the cyclohexa-l-3-diene lactam derivative. This strain, designated CMC
103381, was deposited at NCHVffi Ltd., 23 St. Machar Drive, Aberdeen AB24 3RY, Scotland, on 31.03.99 and again on 29.03.00. The respective accession numbers are
41013 and
Example 2 Cell Paste
A lOμl loopfiil of colony of CMC 103381 (from agar plate) was used to inoculate seed flasks (100ml Tryptone soya broth (Oxoid CM 129) per 500ml Erlenmeyer flask). These were shaken at 25°C, 3 OOrpm in a temperature-controlled shaker (New Brunswick
G-25 with 25 mm (1 inch) throw) for 23 hours. A 0.67% v/v inoculum was used to inoculate the fermenters. The fermenters used were 3.01 Applikon fermenters containing
1.5L medium per fermenter. The fermentation medium contained, per litre: 15g yeast extract (Oxoid L21), 8g KH2PO4, 7g K2HPO , lg MgSO4.7H2O, lg (NH4)2SO4, 1ml trace elements solution, 1.0ml polypropylene glycol (Merck 29767 6Y), and 20g glucose. The trace elements solution contained, per litre: 250ml cone. HC1, 3.6g CaCl2.2H20, 2.0gZnO, 0.85g CuCl2.2H2O, 2.0g MnCl2.4H2O, 5.4g FeCl3.6H2O, 2.4g CoCl2.2H2O, 4.8g Na2MoO4.2H2O, and 0.3g H3BO3. Control was with Biolab II controllers (Brighton systems). The fermenters were grown at 25°C, pH control between 6.9 and 7.1 (with 5M NaOH H3PO4), 800 rpm, air flow at 1.Oml.min"1 for 40 hours. Cell pastes were harvested by centrifugation at 9,000g, 4°C for 20 minutes and were stored at -20°C for future use. Example 3 exo-3-Aza-tricyclo[4.2.1.02'5Jnon-7-en-4-one
A solution of 20.3g norbornadiene in 100ml dichloromethane was slowly added, with stirring, to a solution of 19.1ml chlorosulfonyl isocyanate in 40ml dichloromethane. Upon addition, the solution turned red and gradually darkened to a deep purple colour. After the addition was complete, the mixture was left stirring for a further 30 minutes. The solution was then slowly added to a stirred mixture of 75ml of 33% sodium sulfite solution and 50ml dichloromethane, keeping the temperature at 25°C. The reaction mixture was left stirring for a further 30 minutes after the addition was complete. The organic layer was then separated from the aqueous and dried over magnesium sulfate. Removal of solvent by rotary evaporator yielded 18g, 61%, of a white solid. GC-MS : indicated m z: 135 (M+), 107, 91, 70.
Example 4 Bioresolution of __α-»-3-AzatricycIo[4.2.1.02,s]non-7-en-4-one
Into a 125ml conical flask were placed 2g lactam, 40ml of 50mM phosphate buffer, pH 7 and 0.5g P. putida CMC 103381. The reaction vessel was placed inside a heated jacket to ensure that the temperature remained at 25°C. The reaction mixture was gently stirred and, after 24 hours, a further 1.5g of P. putida cells were added to the reaction mixture. After a total reaction time of 39 hours, an aliquot from the reaction was found by chiral GC to contain only a single enantiomer of the cyclic β-lactam. The reaction was halted after 46 hours, the enzyme cells were spun out by centrifugation (3400 rpm) and the resulting pellet was washed with distilled water and respun. The supernatant was extracted with 2 x 70ml ethyl acetate, the organic extracts were combined and dried over magnesium sulfate. Removal of solvent by rotary evaporator yielded 770mg of a
white solid, [α]D = -91° (20°C, MeOH). Chiral GC analysis was carried out using the Chirasil Dex-CB column: Retention time: 4.77 minutes, enantiomeric excess >95%. Example 5 7-AzabicycIo[4.2.0]-oct-4-en-8-one
A solution of 10ml of cyclohexa-l,3-diene in 35ml of dichloromethane was slowly added, with stirring, to a solution of 7.4ml of chlorosulfonyl isocyanate in 150ml of dichloromethane. Upon addition, the solution turned red and gradually darkened to a deep purple colour. After the addition was complete, the mixture was left stirring for a further 5 minutes. The solution was then slowly added to a stirred mixture of 100ml of 25% sodium sulfite solution and 50ml dichloromethane. The reaction mixture was left stirring for a further 20 minutes after the addition was complete. The organic layer was then separated from the aqueous and dried over magnesium sulfate. Removal of solvent by rotary evaporator yielded 3.4g, 33%, of a yellow oil which solidified on standing. GC-MS : indicated m/z: 123 (M+), 94, 80
'H NMR (CDC13): 6.3-5.8 (2H; m), 4.1 (1H; t), 3.5 (1H; brs), 2.3-1.3 (4H; m). Example 6 Bioresolution of 7-azabicyclo[4.2.0J-oct-4-en-8-one
Into a 100ml conical flask were placed 1.49g lactam, 40ml of 50mM phosphate buffer, pH 7 and 1.4 lg E. putida CMC 103381. The reaction vessel was placed within a heated jacket and the temperature was maintained at 30°C. The reaction mixture was stirred continuously for 22 hours, after which time an aliquot was taken and analysed by chiral GC to determine whether the resolution was complete. The aliquot was extracted with ethyl acetate and derivatised using TFAA. The resulting chiral GC chromatogram indicated lactam with an e.e. of 93%. The reaction was halted, enzyme cells were spun off by centrifuge (3400 rpm) and the supernatant was extracted with 3 x 50ml of ethyl acetate. The organic phase was dried over magnesium sulfate and solvent was removed by rotary evaporator to yield 460mg of an off-white solid, [α]D= -105.5° (20°C, MeOH).
Chiral GC analysis was carried out using a Chirasil Dex-CB column: Retention times: 15.46 mins (major enantiomer), 15.79 mins (minor enantiomer). Enantiomeric excess: 93%.
The amino acid produced from the bioresolution of 7-azabicyclo[4.2.0]-oct-4-en-8- one was isolated in two steps as the hydrochloride salt.
The 15ml of buffered solution remaining from the bioresolution described above contain lg (7.1 mmol) of amino acid (maximum). To the stirred buffer solution was slowly
added, at IOC, 1.55g (7.1mmol) dibutyl dicarbonate dissolved in 20ml THF. The pH of the solution was maintained at 9 by addition of 3M NaOH solution. The reaction mixture was allowed to warm up to room temperature and left stirring overnight. The reaction was halted after 18 hours and THF was removed by rotary evaporator. The aqueous reaction mixture was acidified to pH3 with 10% potassium hydrogen sulfate solution. The mixture was then extracted with 3 x 50ml of ethyl acetate and the combined organic extracts were dried over magnesium sulfate. Removal of solvent by rotary evaporator yielded 1.1 g, 62% of a white solid.
Into a 25ml conical flask was placed 0.71g (2.81mmol) of Boc-protected amino acid, dissolved in 15ml THF. The stirred reaction mixture was acidified to pH 3 with 3M HC1 solution and the reaction was vigorously stirred for 3 hours at ambient temperature. The reaction mixture was left to stand overnight and extracted with 10ml ethyl acetate. The aqueous phase was concentrated under vacuum to yield 480mg, 87% yield of an off- white solid. ΗNMR (CD3OD): 6.05-5.85 (1H; m), 5.65-5.50 (1H; m), 3.9 (1H; brs), 2.85 (1H; dt), 2.15-1.65 (4H; m). Example 7 6-Azabicyclo[3.2.0Jhept-3-en-7-one
Cyclopentadiene was freshly prepared by thermolysis of cyclopentadiene dimer.
It was stored at -20°C, until required to prevent dimerisation. A solution of cyclopentadiene (1.65g, 25mmol) in dichloromethane (15ml) was slowly added to a stirred solution of chlorosulphonyl isocyanate (1.8ml, 21mmol) in dichloromethane (35ml) at
-20°C for 15 minutes. Upon the addition the solution turned to dark red. The temperature was kept -20°C. After a further 20 minutes, a solution of anhydrous sodium bisulfite (6g in 25ml water) was added dropwise to the stirred solution. After 30 minutes the organic layer was separated and the aqueous layer was extracted twice with dichloromethane
( 15ml). The combined organic phases were dried over magnesium sulfate then evaporated.
1H-NMR showed a 55/1 mixture of the 6-azabicyclo[3.2.0]hept-3-en-7-one and the 2- azabicyclo[2.2.1]hept-5-en-3-one. The products were separated by chromatography on silica gel. 6-Azabicyclo[3.2.0]hept-3-en-7-one was isolated as a yellow oil (0.95g, 40%). Example 8 Bioresolution of racemic 6-azabicyclo[3.2.0]hept-3-en-7-one
In a jacketed vessel at 30°C were placed 10ml phosphate buffer (50 mM, pH 7), 200mg 6-azabicyclo[3.2.0]hept-3-en-7-one and P. putida CMC103381 (200mg). The
reaction was stirred overnight. The ee of the residual substrate was assayed by chiral gas chromatography using a TFAA-derivatised sample. An aliquot was taken after 22h, derivatised using TFAA and analysed by GC.
Chiral Gas Chromatography, column Chirasil Dex CB
Racemate : enantiomer 1 retention = 4.09 min, enantiomer 2 t = 4.40 min.
Sample of the bioresolution reaction : GC chromatogram revealed almost exclusively single enantiomer (enantiomer 1 1 = 4.09 min), giving residual substrate of >98%ee.
Claims
1. A process for the preparation of an enantiomerically enriched β-lactam, which comprises enantioselective hydrolysis of the corresponding racemic β-lactam in the presence of a lactamase enzyme capable of enantioselective hydrolysis of 3- azatricyclo[4.2.1.02'5]non-7-en-4-one and 7-azabicyclo[4.2.0]oct-4-en-8-one.
2. A process according to claim 1, wherein the lactamase enzyme is in isolated and purified form.
3. A process according to claim 1 , wherein the lactamase enzyme is in the form of a cell paste or intact cells.
4. A process according to any preceding claim, which additionally comprises isolation of the enantiomerically enriched β-amino acid produced by hydrolysis.
5. A process according to claim 4, wherein the isolated β-amino acid is then subjected to a condensation reaction to reform the β-lactam ring.
6. A process according to any preceding claim, wherein the lactam is a fused polycyclic compound of the type represented by formula (1)
wherein ring A is any monocyclic or any polycyclic ring, optionally substituted with one or more non-interfering groups.
7. A process according to claim 6, wherein the lactam has the formula
wherein ring A is unsaturated and optionally also bridged or further fused.
8. A process according to claim 7, wherein the lactam is 7-en-4-one (2)
(2)
9. A process according to claim 1 , wherein the lactam is 7-azabicyclo[4.2.0]oct-4-en- 8-one (3)
(3)
10. A process according to any preceding claim, wherein the lactamase enzyme is obtainable from a microorganism having the characteristics of that available as the Pseudomonas putida strain identified as CMC 103381, Accession No. NCIMB
11. Enantiomerically enriched 3-azatricyclo[4.2.1.02,5]non-7-en-4-one of formula (2) as shown in claim 8, in an enantiomeric excess of at least 80%.
12. Enantiomerically enriched 7-azabicyclo[4.2.0]oct-4-en-8-one of formula (3) as shown in claim 9, in an enantiomeric excess of at least 80%.
13. A lactam according to claim 11 or claim 12, wherein the enantiomeric excess is at least 95%.
14. Enantiomerically enriched levorotatory enantiomer according to any of claims 11 to 13.
15. A lactamase enzyme obtainable from a microorganism having the characteristics of that available as the Pseudomonas putida strain identified as CMC103381, Accession No. NCIMB
16. Pseudomonas putida strain identified as CMC 103381, Accession No. NCIMB AMENDED CLAIMS
[received by the International Bureau on 4 September 2000 (04.09.00); original claims 10, 15 and 16 amended; remaining claims unchanged (1 page)]
8. A process according to claim 7. wherein the lactam is
7-en-4-one (2)
9. A process according to claim 1 , wherein the lactam is 7-azabicyclo[4.2.0]oct-4-en-
8-one (3)
(3)
10. (amended) A process according to any preceding claim, wherein the lactamase enzyme is obtainable from a microorganism having the characteristics of that available as the Rhodococcus globerulus strain identified as CMC 103381 , Accession No . NCIMB 41042.
11. Enantiomerically enriched 3-azatricyclo[4.2.1.02,5]non-7-en-4-one of formula (2) as shown in claim 8, in an enantiomeric excess of at least 80%.
12. Enantiomerically enriched 7-azabicyclo[4.2.0]oct-4-en-8-one of formula (3) as shown in claim 9, in an enantiomeric excess of at least 80%.
13. A lactam according to claim 11 or claim 12, wherein the enantiomeric excess is at least 95%.
14. Enantiomerically enriched levorotatory enantiomer according to any of claims 11 to 13.
15. (amended) A lactamase enzyme obtainable from a microorganism having the characteristics of that available as the Rhodococcus globerulus strain identified as
CMC103381, Accession No. NCIMB 41042. 1 ό.(amended) Rhodococcus globerulus strain identified as CMC 103381 , Accession No.
NCIMB 41042. Statement under Article 19(1)
Claims 10, 15 and 16 have been amended, in order to give the accession number (not available on filing) and the correct taxonomic name for the deposited strain. In support of these amendments, I refer to my letter dated 12th July and also to the attached copies of a Declaration and receipt issued by NCIMB.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB9907415 | 1999-03-31 | ||
GBGB9907415.5A GB9907415D0 (en) | 1999-03-31 | 1999-03-31 | Biocatalyst and its use |
PCT/GB2000/001198 WO2000058283A1 (en) | 1999-03-31 | 2000-03-29 | Biocatalyst and its use in enzymatic resolution of racemic beta-lactams |
Publications (1)
Publication Number | Publication Date |
---|---|
EP1165507A1 true EP1165507A1 (en) | 2002-01-02 |
Family
ID=10850715
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP00918984A Withdrawn EP1165507A1 (en) | 1999-03-31 | 2000-03-29 | Biocatalyst and its use in enzymatic resolution of racemic beta-lactams |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP1165507A1 (en) |
JP (1) | JP2002539813A (en) |
AU (1) | AU3974900A (en) |
CA (1) | CA2360944A1 (en) |
GB (1) | GB9907415D0 (en) |
WO (1) | WO2000058283A1 (en) |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE1807495A1 (en) * | 1968-11-07 | 1970-06-11 | Hoechst Ag | Process for the separation of binary mixtures |
EP0002564B1 (en) * | 1977-11-12 | 1984-06-20 | Beecham Group Plc | Derivatives of 7-oxo-1-azabicyclo(3.2.0)-hept-2-ene-2-carboxylic acid, their preparation, pharmaceutical compositions containing them and intermediates |
WO1987004429A1 (en) * | 1986-01-23 | 1987-07-30 | The Upjohn Company | Antimicrobial n-acyl-2-azetidinones |
DE69016739T2 (en) * | 1989-10-16 | 1995-06-14 | Chiroscience Ltd | Chiral azabicycloheptanones and process for their preparation. |
GB9108384D0 (en) * | 1991-04-19 | 1991-06-05 | Enzymatix Ltd | Bicycloheptanes |
AU6988996A (en) * | 1995-09-21 | 1997-04-09 | Novartis Ag | 7-azabicyclo(4.2.0)oct-4-en-8-one derivates as plant-microbicides |
JP2003517266A (en) * | 1998-02-17 | 2003-05-27 | ジー・ディー・サール・アンド・カンパニー | Lactam enzymatic resolution method |
-
1999
- 1999-03-31 GB GBGB9907415.5A patent/GB9907415D0/en not_active Ceased
-
2000
- 2000-03-29 CA CA002360944A patent/CA2360944A1/en not_active Abandoned
- 2000-03-29 AU AU39749/00A patent/AU3974900A/en not_active Abandoned
- 2000-03-29 JP JP2000607986A patent/JP2002539813A/en active Pending
- 2000-03-29 WO PCT/GB2000/001198 patent/WO2000058283A1/en not_active Application Discontinuation
- 2000-03-29 EP EP00918984A patent/EP1165507A1/en not_active Withdrawn
Non-Patent Citations (1)
Title |
---|
See references of WO0058283A1 * |
Also Published As
Publication number | Publication date |
---|---|
GB9907415D0 (en) | 1999-05-26 |
WO2000058283A1 (en) | 2000-10-05 |
CA2360944A1 (en) | 2000-10-05 |
JP2002539813A (en) | 2002-11-26 |
AU3974900A (en) | 2000-10-16 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP0424064B1 (en) | Chiral azabicyloheptanone and a process for their preparation | |
Mollica et al. | Synthesis of (S)-5, 6-dibromo-tryptophan derivatives as building blocks for peptide chemistry | |
Garcı́a-Urdiales et al. | Enzymatic ammonolysis of ethyl (±)-4-chloro-3-hydroxybutanoate. Chemoenzymatic syntheses of both enantiomers of pyrrolidin-3-ol and 5-(chloromethyl)-1, 3-oxazolidin-2-one | |
US5284769A (en) | Process for preparing a single enantiomer of a lactam using lactamase | |
JP6145538B2 (en) | Process for the production of (3aS, 7aR) -hexahydroisobenzofuran-1 (3H) -one by biological resolution of dimethyl cyclohexane-1,2-dicarboxylate using a catalyst | |
KR100479894B1 (en) | Process for Preparing Enantiomerically Enriched N-derivatised Lactams | |
WO2000058283A1 (en) | Biocatalyst and its use in enzymatic resolution of racemic beta-lactams | |
JP2010532992A (en) | Microbial kinetic resolution of ethyl 3,4-epoxybutyrate | |
EP1002871A1 (en) | Process for preparing optically active 3-hydroxy-pyrrolidine derivatives by enzymatic hydroxylation | |
JP4843813B2 (en) | Method for preparing R- or S-form α-substituted heterocyclic carboxylic acid and enantiomer of α-substituted heterocyclic carboxylic acid ester of the opposite mirror image using enzyme | |
Bertoli et al. | Chemoenzymatic synthesis of optically active α-methylene-γ-carboxy-γ-lactams and γ-lactones | |
JP3650648B2 (en) | Preparation of enantiomerically pure lactam | |
JP2003534807A (en) | Optical resolution of racemic α-substituted heterocyclic carboxylic acids using enzymes | |
Sigmund et al. | Enantioselective enzymatic aminolysis of a racemic 2-isoxazolylacetate alkyl ester | |
EP0407190A2 (en) | Process for producing antibacterial intermediates via enzyme hydrolysis of racemic substrates | |
AU2014200205B2 (en) | Preparation of (3aS,7aR)-hexahydroisobenzofuran-1(3H)-one by catalyzed biological resolution of dimethyl cyclohexane-1,2-dicarboxylate | |
Lloyd et al. | A concise synthesis of single‐enantiomer β‐lactams and β‐amino acids using Rhodococcus globerulus | |
Kamal et al. | Lipase-catalyzed resolution of 1-chloro-3-[(4-morpholin-4-yl-1, 2, 5-thiadiazole-3-yl) oxy] propan-2-ol: Synthesis of (R)-and (S)-timolol | |
Tarui et al. | Microbial enantioselective ester hydrolysis for the preparation of optically active 4, 1-benzoxazepine-3-acetic acid derivatives as squalene synthase inhibitors | |
Klempier et al. | Nitrilase‐and Nitrile Hydratase‐Catalyzed Enantioselective Preparation of Non‐Proteinogenic Amino Acids | |
MXPA99011966A (en) | Process for preparing enantiomerically enriched n-derivatised lactams |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
17P | Request for examination filed |
Effective date: 20011015 |
|
AK | Designated contracting states |
Kind code of ref document: A1 Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE |
|
AX | Request for extension of the european patent |
Free format text: AL;LT;LV;MK;RO;SI |
|
17Q | First examination report despatched |
Effective date: 20021025 |
|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN |
|
18D | Application deemed to be withdrawn |
Effective date: 20040527 |