EP1159414A4 - Verfahren zur steigerung der durch ein elektrisches feld vermittelten einbringung biologischen materials in zellen - Google Patents

Verfahren zur steigerung der durch ein elektrisches feld vermittelten einbringung biologischen materials in zellen

Info

Publication number
EP1159414A4
EP1159414A4 EP00964909A EP00964909A EP1159414A4 EP 1159414 A4 EP1159414 A4 EP 1159414A4 EP 00964909 A EP00964909 A EP 00964909A EP 00964909 A EP00964909 A EP 00964909A EP 1159414 A4 EP1159414 A4 EP 1159414A4
Authority
EP
European Patent Office
Prior art keywords
cells
electric field
biological materials
mediated delivery
enhancing electric
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP00964909A
Other languages
English (en)
French (fr)
Other versions
EP1159414A1 (de
Inventor
Alan D King
Robert W Malone
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cyto Pulse Sciences Inc
University of Maryland at Baltimore
Original Assignee
Cyto Pulse Sciences Inc
University of Maryland at Baltimore
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cyto Pulse Sciences Inc, University of Maryland at Baltimore filed Critical Cyto Pulse Sciences Inc
Publication of EP1159414A1 publication Critical patent/EP1159414A1/de
Publication of EP1159414A4 publication Critical patent/EP1159414A4/de
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
EP00964909A 1999-09-24 2000-09-25 Verfahren zur steigerung der durch ein elektrisches feld vermittelten einbringung biologischen materials in zellen Withdrawn EP1159414A4 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US15564099P 1999-09-24 1999-09-24
US155640P 1999-09-24
PCT/US2000/021982 WO2001023537A1 (en) 1999-09-24 2000-09-25 Process for enhancing electric field-mediated delivery of biological materials into cells

Publications (2)

Publication Number Publication Date
EP1159414A1 EP1159414A1 (de) 2001-12-05
EP1159414A4 true EP1159414A4 (de) 2003-08-27

Family

ID=22556208

Family Applications (1)

Application Number Title Priority Date Filing Date
EP00964909A Withdrawn EP1159414A4 (de) 1999-09-24 2000-09-25 Verfahren zur steigerung der durch ein elektrisches feld vermittelten einbringung biologischen materials in zellen

Country Status (3)

Country Link
EP (1) EP1159414A4 (de)
CA (1) CA2390716A1 (de)
WO (1) WO2001023537A1 (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003051454A2 (en) * 2001-12-14 2003-06-26 Genetronics, Inc. Methods for particle-assisted polynucleotide immunization using a pulsed electric field
BRPI0611534A2 (pt) 2005-05-12 2010-09-21 Novartis Ag genes e proteìnas da brachyspira hyodysenteriae e uso das mesmas para diagnose e terapia
MX2010001381A (es) 2007-08-03 2010-09-14 Spirogene Pty Ltd Genes y proteinas de brachyspira hyodysenteriae y usos de los mismos.
GB0720250D0 (en) 2007-10-17 2007-11-28 Univ Edinburgh Immunogenic compositions containing escherichia coli h7 flagella and methods of use thereof
EP2363407A1 (de) 2008-02-28 2011-09-07 Murdoch University Neuartige Brachyspira-Sequenzen, immunogene Zusammensetzungen, Herstellungsverfahren dafür und Verwendung davon
AU2009227986C1 (en) 2008-03-27 2014-06-19 Murdoch University Novel sequences of Brachyspira, immunogenic compositions, methods for preparation and use thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0424688A2 (de) * 1989-09-28 1991-05-02 Iowa State University Research Foundation, Inc. Synergistisches Verfahren zur Wirtszellentransformation
WO1993019768A1 (en) * 1992-04-03 1993-10-14 The Regents Of The University Of California Self-assembling polynucleotide delivery system
WO1996040961A1 (en) * 1995-06-07 1996-12-19 Life Technologies, Inc. Peptide-enhanced cationic lipid transfections
US5945400A (en) * 1995-02-17 1999-08-31 Rhone-Poulenc Rorer Sa Nucleic acid-containing composition, preparation and use thereof

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69727500T2 (de) * 1997-06-10 2004-10-07 Cyto Pulse Sciences Inc Verfahren und apparatur zur behandlung von material mit elektrischen feldern unterschiedlicher orientierung

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0424688A2 (de) * 1989-09-28 1991-05-02 Iowa State University Research Foundation, Inc. Synergistisches Verfahren zur Wirtszellentransformation
WO1993019768A1 (en) * 1992-04-03 1993-10-14 The Regents Of The University Of California Self-assembling polynucleotide delivery system
US5945400A (en) * 1995-02-17 1999-08-31 Rhone-Poulenc Rorer Sa Nucleic acid-containing composition, preparation and use thereof
WO1996040961A1 (en) * 1995-06-07 1996-12-19 Life Technologies, Inc. Peptide-enhanced cationic lipid transfections
US5736392A (en) * 1995-06-07 1998-04-07 Life Technologies, Inc. Peptide-enhanced cationic lipid transfections

Non-Patent Citations (15)

* Cited by examiner, † Cited by third party
Title
BABA MIYAKO ET AL: "In vivo electroporetic transfer of bcl-2 antisense oligonucleotide inhibits the development of hepatocellular carcinoma in rats.", INTERNATIONAL JOURNAL OF CANCER., vol. 85, no. 2, 15 January 2000 (2000-01-15), pages 260 - 266, XP002245188, ISSN: 0020-7136 *
BOUSSIF O ET AL: "A VERSATILE VECTOR FOR GENE AND OLIGONUCLEOTIDE TRANSFER INTO CELLS IN CULTURE AND IN VIVO: POLYETHYLENIMINE", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 92, no. 16, August 1995 (1995-08-01), pages 7297 - 7301, XP002006502, ISSN: 0027-8424 *
CHERNOMORDIK L V ET AL: "INCREASED BINDING OF LIPOSOMES TO CELLS BY ELECTRIC TREATMENT", BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1070, no. 1, 1991, pages 193 - 197, XP009012350, ISSN: 0006-3002 *
COULBERSON A L ET AL: "Gene packaging with lipids, peptides and viruses inhibits transfection by electroporation in vitro", JOURNAL OF CONTROLLED RELEASE, ELSEVIER SCIENCE PUBLISHERS B.V. AMSTERDAM, NL, vol. 86, no. 2-3, 17 January 2003 (2003-01-17), pages 361 - 370, XP004401129, ISSN: 0168-3659 *
FELGNER P L: "PARTICULATE SYSTEMS AND POLYMERS FOR IN VITRO AND IN VIVO DELIVERY OF POLYNUCLEOTIDES", ADVANCED DRUG DELIVERY REVIEWS, AMSTERDAM, NL, vol. 5, no. 3, 1 September 1990 (1990-09-01), pages 163 - 187, XP000603153, ISSN: 0169-409X *
GAO X ET AL: "CATIONIC LIPOSOME-MEDIATED GENE TRANSFER", GENE THERAPY, MACMILLAN PRESS LTD., BASINGSTOKE, GB, vol. 2, no. 10, 1 December 1995 (1995-12-01), pages 710 - 722, XP000749400, ISSN: 0969-7128 *
GODBEY W T ET AL: "Poly(ethylenimine) and its role in gene delivery", JOURNAL OF CONTROLLED RELEASE, ELSEVIER SCIENCE PUBLISHERS B.V. AMSTERDAM, NL, vol. 60, no. 2-3, 5 August 1999 (1999-08-05), pages 149 - 160, XP004362929, ISSN: 0168-3659 *
LEDLEY F D: "NONVIRAL GENE THERAPY: THE PROMISE OF GENES AS PHARMACEUTICAL PRODUCTS", HUMAN GENE THERAPY, XX, XX, vol. 6, September 1995 (1995-09-01), pages 1129 - 1144, XP001007448, ISSN: 1043-0342 *
MACCARRONE M ET AL: "Gene transfer to lentil protoplasts by lipofection and electroporation", JOURNAL OF LIPOSOME RESEARCH 1993 UNITED STATES, vol. 3, no. 3, 1993, pages 707 - 716, XP009012606, ISSN: 0898-2104 *
MACHY P ET AL: "Gene transfer from targeted liposomes to specific lymphoid cells by electroporation.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. UNITED STATES NOV 1988, vol. 85, no. 21, November 1988 (1988-11-01), pages 8027 - 8031, XP001152921, ISSN: 0027-8424 *
MACK K D ET AL: "A novel method for DEAE-dextran mediated transfection of adherent primary cultured human macrophages", JOURNAL OF IMMUNOLOGICAL METHODS, ELSEVIER SCIENCE PUBLISHERS B.V.,AMSTERDAM, NL, vol. 211, no. 1-2, 1998, pages 79 - 86, XP004120571, ISSN: 0022-1759 *
SAWAI KEISUKE ET AL: "A novel method of cell-specific mRNA transfection.", MOLECULAR GENETICS AND METABOLISM, vol. 64, no. 1, May 1998 (1998-05-01), pages 44 - 51, XP002245185, ISSN: 1096-7192 *
See also references of WO0123537A1 *
SPOERLEIN B ET AL: "LIPOFECTIN DIRECT GENE TRANSFER TO HIGHER PLANTS USING CATIONIC LIPOSOMES", THEORETICAL AND APPLIED GENETICS, vol. 83, no. 1, 1991, pages 1 - 5, XP009012649, ISSN: 0040-5752 *
WELLS J M ET AL: "ELECTROPORATION-ENHANCED GENE DELIVERY IN MAMMARY TUMORS", GENE THERAPY, MACMILLAN PRESS LTD., BASINGSTOKE, GB, vol. 7, no. 7, April 2000 (2000-04-01), pages 541 - 547, XP008010350, ISSN: 0969-7128 *

Also Published As

Publication number Publication date
CA2390716A1 (en) 2001-04-05
WO2001023537A1 (en) 2001-04-05
EP1159414A1 (de) 2001-12-05

Similar Documents

Publication Publication Date Title
IL161858A0 (en) Cell culture process
AU2002225781A1 (en) Microincubator cell culture apparatus including transparent heater
AU6419800A (en) Hybrid chemical and biological process for decontaminating sludge from municipalsewage
IL143421A0 (en) Method for culturing algae
GB9903472D0 (en) Chemical process
GB9905560D0 (en) Process for the manufacture of supported materials
AU6229498A (en) Composition for gene introduction into cell
AU1455301A (en) Chuck heater for improved planar deposition process
AU6387000A (en) Serial analysis of genetic alterations
EP1159414A4 (de) Verfahren zur steigerung der durch ein elektrisches feld vermittelten einbringung biologischen materials in zellen
GB9926250D0 (en) Chemical process
AU1571801A (en) Improved fermentation process
GB9902422D0 (en) Reference material for nucleic acid amplification
AU6350300A (en) Method for chemical analysis of biological material
IL145826A0 (en) Quantitative assay for expression of genes in microarray
AU4417000A (en) Novel pharmaceutical composition suitable for gene therapy
HUP0200342A2 (en) Novel process for preparing alendronic acid
AU2153401A (en) Gene expression in biological conditions
GB9928674D0 (en) Reagent and method for delivery of molecules into cells
AU2370901A (en) Methods for collecting genetic material
AU5515501A (en) Mammalian cell culture chamber
AU6135499A (en) Process of recycling biological waste
EP1105518A4 (de) VERWENDUNG VON mCRP ZUM EINBRINGEN VON MATERIALIEN IN ZELLEN
EP1127941A4 (de) Dna-fragment, welches die genexpressionsdosis erhöht
AU5257000A (en) Procedure for extraction of lipids from biological material

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20011005

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

RIC1 Information provided on ipc code assigned before grant

Ipc: 7C 12N 15/87 B

Ipc: 7C 12N 15/85 B

Ipc: 7C 12N 15/70 B

Ipc: 7C 12N 15/82 B

Ipc: 7C 12N 15/00 A

A4 Supplementary search report drawn up and despatched

Effective date: 20030710

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20050402