EP1151306A1 - Spectrometrie de masse a haut rendement - Google Patents

Spectrometrie de masse a haut rendement

Info

Publication number
EP1151306A1
EP1151306A1 EP00913451A EP00913451A EP1151306A1 EP 1151306 A1 EP1151306 A1 EP 1151306A1 EP 00913451 A EP00913451 A EP 00913451A EP 00913451 A EP00913451 A EP 00913451A EP 1151306 A1 EP1151306 A1 EP 1151306A1
Authority
EP
European Patent Office
Prior art keywords
cell
samples
enzyme
column
mass spectrometry
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP00913451A
Other languages
German (de)
English (en)
Inventor
Sun Ai Raillard
Yong Hong Chen
Claus Krebber
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Maxygen Inc
Original Assignee
Maxygen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Maxygen Inc filed Critical Maxygen Inc
Publication of EP1151306A1 publication Critical patent/EP1151306A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/02—Details
    • H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un appareil et des procédés permettant de réaliser une spectrométrie de masse à haut rendement. Ces procédés consistent à préparer des consistent, entre autres, à utiliser un tampon approprié pendant la génération d'échantillons ou à utiliser un support solide pour des composants marqués. Les échantillons ainsi préparés n'exigent pas une séparation en colonnes. Cet appareil comporte une plaque de culture cellulaire servant à cultiver des cellules et à obtenir des produits et/ou des réactifs, un système de purification parallèle hors circuit, un spectromètre de masse et un échantillonneur automatique qui transporte les échantillons et les injecte dans le spectromètre de masse. Ces procédés et cet appareil servent, par exemple, à cribler des voies de réaction enzymatique.
EP00913451A 1999-02-11 2000-02-11 Spectrometrie de masse a haut rendement Withdrawn EP1151306A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US11976699P 1999-02-11 1999-02-11
US119766P 1999-02-11
US14884899P 1999-08-12 1999-08-12
US148848P 1999-08-12
PCT/US2000/003686 WO2000048004A1 (fr) 1999-02-11 2000-02-11 Spectrometrie de masse a haut rendement

Publications (1)

Publication Number Publication Date
EP1151306A1 true EP1151306A1 (fr) 2001-11-07

Family

ID=26817677

Family Applications (1)

Application Number Title Priority Date Filing Date
EP00913451A Withdrawn EP1151306A1 (fr) 1999-02-11 2000-02-11 Spectrometrie de masse a haut rendement

Country Status (3)

Country Link
EP (1) EP1151306A1 (fr)
AU (1) AU3489900A (fr)
WO (1) WO2000048004A1 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6680203B2 (en) * 2000-07-10 2004-01-20 Esperion Therapeutics, Inc. Fourier transform mass spectrometry of complex biological samples
EP1404707A4 (fr) * 2001-05-29 2005-02-02 Univ Michigan Systemes et procedes d'analyse de proteines
WO2003073464A1 (fr) * 2002-02-28 2003-09-04 Metanomics Gmbh & Co. Kgaa Procede de spectrometrie de masse pour analyser des melanges de substances
WO2003087834A2 (fr) * 2002-04-08 2003-10-23 Affinium Pharmaceuticals, Inc. Purification, caracterisation et identification a haut rendement de proteines recombinantes
GB0311961D0 (en) 2003-05-23 2003-06-25 Health Prot Agency Mass-based toxin assay and substrates therefor
FR2857451B1 (fr) * 2003-07-11 2005-09-30 Commissariat Energie Atomique Procede et dispositif pour l'analyse de milieux reactionnels vivants
US7833745B2 (en) 2003-09-11 2010-11-16 E. I. Du Pont De Nemours And Company Direct detection method for products of cellular metabolism using ToF-SIMS

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998020020A2 (fr) * 1996-11-06 1998-05-14 Sequenom, Inc. Immobilisation haute densite d'acides nucleiques

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998020020A2 (fr) * 1996-11-06 1998-05-14 Sequenom, Inc. Immobilisation haute densite d'acides nucleiques

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
"Filtration", WIKIPEDIA, pages 1 - 3, Retrieved from the Internet <URL:http://en.wikipedia.org/w/index.php?title=Filtration&printable=yes> [retrieved on 20070403] *
"Mass spectrometry", WIKIPEDIA, pages 1 - 13, Retrieved from the Internet <URL:http://en.wikipedia.org/w/index.php?title=Mass_spectrometry&printable=yes> [retrieved on 20070403] *
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES, vol. 6, no. 1, August 1998 (1998-08-01), pages S93, XP008062899 *
JOURNAL OF CHROMATOGRAPHY B, vol. 713, 1998, pages 3 - 25 *
See also references of WO0048004A1 *

Also Published As

Publication number Publication date
WO2000048004A1 (fr) 2000-08-17
AU3489900A (en) 2000-08-29
WO2000048004A9 (fr) 2001-09-27

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