EP1144669A4 - Method and apparatus for the automated generation of nucleic acid ligands - Google Patents

Method and apparatus for the automated generation of nucleic acid ligands

Info

Publication number
EP1144669A4
EP1144669A4 EP00909909A EP00909909A EP1144669A4 EP 1144669 A4 EP1144669 A4 EP 1144669A4 EP 00909909 A EP00909909 A EP 00909909A EP 00909909 A EP00909909 A EP 00909909A EP 1144669 A4 EP1144669 A4 EP 1144669A4
Authority
EP
European Patent Office
Prior art keywords
nucleic acid
acid ligands
automated generation
automated
generation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP00909909A
Other languages
German (de)
French (fr)
Other versions
EP1144669A1 (en
Inventor
Larry Gold
Dominic A Zichi
Robert Jenison
Daniel J Schneider
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Somalogic Inc
Original Assignee
Somalogic Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/232,946 external-priority patent/US6569620B1/en
Application filed by Somalogic Inc filed Critical Somalogic Inc
Publication of EP1144669A1 publication Critical patent/EP1144669A1/en
Publication of EP1144669A4 publication Critical patent/EP1144669A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1048—SELEX
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B01J2219/00315—Microtiter plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00364—Pipettes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/005—Beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00686—Automatic
    • B01J2219/00689—Automatic using computers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00686—Automatic
    • B01J2219/00691—Automatic using robots
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00702—Processes involving means for analysing and characterising the products
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
EP00909909A 1999-01-19 2000-01-14 Method and apparatus for the automated generation of nucleic acid ligands Withdrawn EP1144669A4 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US09/232,946 US6569620B1 (en) 1990-06-11 1999-01-19 Method for the automated generation of nucleic acid ligands
US232946 1999-01-19
US35623399A 1999-07-16 1999-07-16
US356233 1999-07-16
PCT/US2000/001001 WO2000043534A1 (en) 1999-01-19 2000-01-14 Method and apparatus for the automated generation of nucleic acid ligands

Publications (2)

Publication Number Publication Date
EP1144669A1 EP1144669A1 (en) 2001-10-17
EP1144669A4 true EP1144669A4 (en) 2005-01-05

Family

ID=26926487

Family Applications (1)

Application Number Title Priority Date Filing Date
EP00909909A Withdrawn EP1144669A4 (en) 1999-01-19 2000-01-14 Method and apparatus for the automated generation of nucleic acid ligands

Country Status (7)

Country Link
EP (1) EP1144669A4 (en)
JP (1) JP2002534985A (en)
KR (1) KR20010101573A (en)
AU (1) AU777823B2 (en)
CA (1) CA2360748A1 (en)
MX (1) MXPA01007352A (en)
WO (1) WO2000043534A1 (en)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001096559A2 (en) * 2000-06-15 2001-12-20 Board Of Regents The University Of Texas System Regulatable, catalytically active nucleic acids
EP1340082A2 (en) * 2000-08-23 2003-09-03 Imego AB Microfluidic device and method with transport of magnetic particles into and out of wells
US20020048821A1 (en) 2000-08-24 2002-04-25 David Storek Sample preparing arrangement and a method relating to such an arrangement
US7998746B2 (en) 2000-08-24 2011-08-16 Robert Otillar Systems and methods for localizing and analyzing samples on a bio-sensor chip
US7179659B2 (en) 2001-04-02 2007-02-20 Agilent Technologies, Inc. Sensor surfaces for detecting analytes and methods of use
EP1386138A4 (en) * 2001-04-02 2005-05-18 Agilent Technologies Inc Systems and apparatus for the analysis of molecular interactions
CN1653338A (en) * 2002-05-17 2005-08-10 贝克顿·迪金森公司 Automated system for isolating, amplifying and detecting a target nucleic acid sequence
EP1543158B1 (en) 2002-07-25 2011-07-13 Archemix Corp. Regulated aptamer therapeutics
DE10241938A1 (en) * 2002-09-10 2004-03-25 Noxxon Pharma Ag Selecting specific-bonding nucleic acid, by a modified SELEX process that includes purification by ultrafiltration to facilitate automation
JP5156905B2 (en) * 2007-04-20 2013-03-06 独立行政法人科学技術振興機構 Aptamer generating apparatus and aptamer generating method
JP2011500076A (en) * 2007-10-22 2011-01-06 プロノタ・エヌブイ How to select aptamers
JP5393610B2 (en) * 2010-07-28 2014-01-22 株式会社日立ハイテクノロジーズ Nucleic acid analyzer
US9175332B2 (en) 2010-07-29 2015-11-03 Roche Molecular Systems, Inc. Generic PCR
WO2012013732A1 (en) * 2010-07-29 2012-02-02 F. Hoffmann - La Roche Ag Generic pcr
EP2426222A1 (en) * 2010-09-07 2012-03-07 F. Hoffmann-La Roche AG Generic buffer for amplification
DE102013200193A1 (en) 2013-01-09 2014-07-10 Hamilton Bonaduz Ag Sample processing system with dosing device and thermocycler
CN103614291A (en) * 2013-11-13 2014-03-05 戴小波 Full-automatic fluorescence quantitative gene amplification instrument
CN114276896B (en) * 2021-12-22 2023-12-29 成都瀚辰光翼科技有限责任公司 Automatic nucleic acid extraction method and storage medium
CN114923839B (en) * 2022-07-18 2022-10-28 高分(北京)生物科技有限公司 Full-automatic ultrahigh-flux cell imaging counter and sample detection method

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0236069A2 (en) * 1986-02-25 1987-09-09 The Perkin-Elmer Corporation Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
WO1991019813A1 (en) * 1990-06-11 1991-12-26 The University Of Colorado Foundation, Inc. Nucleic acid ligands
WO1995008003A1 (en) * 1993-09-17 1995-03-23 University Research Corporation Systematic evolution of ligands by exponential enrichment: photoselection of nucleic acid ligands and solution selex
WO1996034874A1 (en) * 1995-05-03 1996-11-07 Nexstar Pharmaceuticals, Inc. Nucleic acid ligands of tissue target
WO1998033941A1 (en) * 1997-01-31 1998-08-06 Nexstar Pharmaceuticals, Inc. Flow cell selex

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69126690T2 (en) * 1990-04-06 1998-01-02 Perkin Elmer Corp AUTOMATED LABORATORY FOR MOLECULAR BIOLOGY
US5985548A (en) * 1993-02-04 1999-11-16 E. I. Du Pont De Nemours And Company Amplification of assay reporters by nucleic acid replication
US5737498A (en) * 1995-07-11 1998-04-07 Beckman Instruments, Inc. Process automation method and apparatus
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0236069A2 (en) * 1986-02-25 1987-09-09 The Perkin-Elmer Corporation Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
WO1991019813A1 (en) * 1990-06-11 1991-12-26 The University Of Colorado Foundation, Inc. Nucleic acid ligands
US5843653A (en) * 1990-06-11 1998-12-01 Nexstar Pharmaceuticals, Inc. Method for detecting a target molecule in a sample using a nucleic acid ligand
WO1995008003A1 (en) * 1993-09-17 1995-03-23 University Research Corporation Systematic evolution of ligands by exponential enrichment: photoselection of nucleic acid ligands and solution selex
WO1996034874A1 (en) * 1995-05-03 1996-11-07 Nexstar Pharmaceuticals, Inc. Nucleic acid ligands of tissue target
WO1998033941A1 (en) * 1997-01-31 1998-08-06 Nexstar Pharmaceuticals, Inc. Flow cell selex

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO0043534A1 *

Also Published As

Publication number Publication date
KR20010101573A (en) 2001-11-14
CA2360748A1 (en) 2000-07-27
AU3209200A (en) 2000-08-07
WO2000043534A1 (en) 2000-07-27
EP1144669A1 (en) 2001-10-17
AU777823B2 (en) 2004-11-04
JP2002534985A (en) 2002-10-22
MXPA01007352A (en) 2003-06-06

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