EP1127619B1 - Dispositif d'un thermocycleur pour réactions d'amplification par polymèrase - Google Patents

Dispositif d'un thermocycleur pour réactions d'amplification par polymèrase Download PDF

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Publication number
EP1127619B1
EP1127619B1 EP01112394A EP01112394A EP1127619B1 EP 1127619 B1 EP1127619 B1 EP 1127619B1 EP 01112394 A EP01112394 A EP 01112394A EP 01112394 A EP01112394 A EP 01112394A EP 1127619 B1 EP1127619 B1 EP 1127619B1
Authority
EP
European Patent Office
Prior art keywords
sample
assembly
temperature
block
sample block
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
EP01112394A
Other languages
German (de)
English (en)
Other versions
EP1127619A3 (fr
EP1127619A2 (fr
Inventor
John G. Atwood
Adrian Fawcett
Keith S. Ferrara
Paul M. Hetherington
Richard W. Noreiks
Douglas E. Olsen
John R. Widomski
Charles M. Wittmer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems LLC
Applied Biosystems Inc
Original Assignee
PE Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to EP09016131.6A priority Critical patent/EP2186568B1/fr
Priority to EP03022672A priority patent/EP1386666B1/fr
Priority to EP14198058.1A priority patent/EP2913109A1/fr
Priority to EP09013036.0A priority patent/EP2156892B1/fr
Priority to EP09006229A priority patent/EP2090366B1/fr
Application filed by PE Corp filed Critical PE Corp
Priority claimed from EP98913265A external-priority patent/EP0963250B1/fr
Publication of EP1127619A2 publication Critical patent/EP1127619A2/fr
Publication of EP1127619A3 publication Critical patent/EP1127619A3/fr
Application granted granted Critical
Publication of EP1127619B1 publication Critical patent/EP1127619B1/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50853—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1822—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using Peltier elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Claims (14)

  1. Assemblage pour soumettre à des cycles des récipients de mélanges réactionnels à travers une série d'excursions de température, comprenant:
    un bloc d'échantillon (36) destiné à recevoir des récipients de mélanges réactionnels;
    une pluralité de dispositifs thermoélectriques (60);
    un dissipateur de chaleur (34);
    un mécanisme de fixation positionné de manière à fixer lesdits dispositifs thermoélectriques entre ledit bloc d'échantillon et ledit dissipateur de chaleur ;
    un radiateur (74) positionné autour du périmétre du dit bloc d'échantillon; et
    une pointe (24) ayant une première extrémité et une deuxième extrémité, ladite première extrémité en contact étroit avec ledit bloc d'échantillon et ladite deuxième extrémité en contact étroit avec ledit dissipateur de chaleur de manière à procurer un trajet thermique entre ledit bloc d'échantillon et ledit dissipateur de chaleur, tandis que ladite pointe (24) est positionnée pour réduire les gradients thermiques à travers ledit-bloc d'échantillon (36).
  2. Assemblage selon la revendication 1, dans lequel ladite pointe (24) est positionnée au centre du dit bloc d'échantillon (36).
  3. Assemblage selon l'une quelconque des revendications 1 à 2, dans lequel ledit dissipateur de chaleur (34) comprend :
    une plaque ayant une face supérieure et une face inférieure;
    une pluralité d'ailettes (37) s'étendant perpendiculairement à partir de ladite face inférieure ;
    une rigole (44) s'étendant autour du périmètre de ladite face supérieure pour empêcher une perte de chaleur à partir du dit périmètre; et
    une niche (40) dans ladite plaque pour recevoir un capteur de température (38).
  4. Assemblage selon la revendication 3, comprenant en outre un ventilateur placé à proximité étroite des dites ailettes (37) pour le réglage de l'écoulement d'air à travers lesdites ailettes.
  5. Assemblage selon l'une quelconque des revendications 1 à 4, dans lequel ledit mécanisme de fixation comprend:
    une pique (49) et ladite pique ayant une pluralité d'orifices (53) dans ladite pique pour recevoir des éléments de fixation (52);
    une pluralité de doigts (48) s'étendant latéralement à partir de ladite pique.
  6. Assemblage selon la revendication 5, dans lequel ladite pique (49) est de forme rectangulaire.
  7. Assemblage selon la revendication 5, dans lequel lesdits doigts (48) sont de forme rectangulaire.
  8. Assemblage selon la revendication 5, dans lequel lesdits doigts (48) ont un point haut et un point bas et sont biseautés de manière à avoir une moindre largeur au dit point haut qu'au dit point bas.
  9. Assemblage selon la revendication 5, dans lequel lesdits doigts (48) ont une première extrémité dépassant latéralement à partir de ladite pique (49) et une saillie s'étendant vers le bas à partir de ladite première extrémité.
  10. Assemblage selon l'une quelconque des revendications 1 à 9; dans lequel leditbloc d'échantillon (36) comprend:
    une pluralité de puits d'échantillon (20), pour la réception de récipients d'échantillon, chaque puits ayant un sommet et un fond ;
    une plaque support supérieure (21) reliant les sommets des dits puits d'échantillon ; et
    une plaque inférieure (22) reliant le fond des dits puits d'échantillon.
  11. Assemblage selon la revendication 10, dans lequel ladite plaque support supérieure (21) et lesdits puits d'échantillon (20) sont électroformés en une seule pièce.
  12. Assemblage selon la revendication 10, dans lequel ledit bloc d'échantillons (36) comprend de l'argent.
  13. Assemblage selon la revendication 10, dans lequel lesdits puits d'échantillon (20) sont disposés en un arrangement de 8 x 12.
  14. Assemblage selon la revendication 10, dans lequel ledit bloc d'échantillon (36) est rectangulaire.
EP01112394A 1997-03-28 1998-03-30 Dispositif d'un thermocycleur pour réactions d'amplification par polymèrase Expired - Lifetime EP1127619B1 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP03022672A EP1386666B1 (fr) 1997-03-28 1998-03-30 Ameliorations apportees à un cycleur thermique pour PCR
EP14198058.1A EP2913109A1 (fr) 1997-03-28 1998-03-30 Ensemble thermocycleur pour PCR
EP09013036.0A EP2156892B1 (fr) 1997-03-28 1998-03-30 Cycleur thermique pour PCR
EP09006229A EP2090366B1 (fr) 1997-03-28 1998-03-30 Améliorations dans le cycle thermique pour PCR
EP09016131.6A EP2186568B1 (fr) 1997-03-28 1998-03-30 Ensemble pour cycleur thermique pour PCR

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US4175497P 1997-03-28 1997-03-28
US41754P 1997-03-28
US4612297P 1997-05-09 1997-05-09
US46122P 1997-05-09
EP98913265A EP0963250B1 (fr) 1997-03-28 1998-03-30 Ameliorations apportees a un cycleur thermique pour pcr

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
EP98913265A Division EP0963250B1 (fr) 1997-03-28 1998-03-30 Ameliorations apportees a un cycleur thermique pour pcr

Related Child Applications (3)

Application Number Title Priority Date Filing Date
EP09016131.6A Division EP2186568B1 (fr) 1997-03-28 1998-03-30 Ensemble pour cycleur thermique pour PCR
EP03022672A Division EP1386666B1 (fr) 1997-03-28 1998-03-30 Ameliorations apportees à un cycleur thermique pour PCR
EP14198058.1A Division EP2913109A1 (fr) 1997-03-28 1998-03-30 Ensemble thermocycleur pour PCR

Publications (3)

Publication Number Publication Date
EP1127619A2 EP1127619A2 (fr) 2001-08-29
EP1127619A3 EP1127619A3 (fr) 2001-09-05
EP1127619B1 true EP1127619B1 (fr) 2003-10-08

Family

ID=27239812

Family Applications (1)

Application Number Title Priority Date Filing Date
EP01112394A Expired - Lifetime EP1127619B1 (fr) 1997-03-28 1998-03-30 Dispositif d'un thermocycleur pour réactions d'amplification par polymèrase

Country Status (1)

Country Link
EP (1) EP1127619B1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2198968A1 (fr) * 2008-12-16 2010-06-23 F. Hoffmann-Roche AG Compositions et procédés pour compléter des puits souterrains

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10228431B4 (de) * 2002-06-26 2004-08-26 Eppendorf Ag Laborprobentemperiervorrichtung mit Aufnahmen
US20040072334A1 (en) * 2002-10-15 2004-04-15 The Regents Of The University Of California Thermal cycler
WO2007149696A1 (fr) † 2006-06-23 2007-12-27 Applera Corporation Systèmes et procédés de refroidissement dans des instruments d'analyses biologiques

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4865986A (en) * 1988-10-06 1989-09-12 Coy Corporation Temperature control apparatus
KR100236506B1 (ko) * 1990-11-29 2000-01-15 퍼킨-엘머시터스인스트루먼츠 폴리머라제 연쇄 반응 수행 장치
CA2130013C (fr) * 1993-09-10 1999-03-30 Rolf Moser Appareil servant a modifier automatiquement la temperature du contenu de differentes eprouvettes
US5410130A (en) * 1994-04-20 1995-04-25 Ericomp, Inc. Heating and temperature cycling

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2198968A1 (fr) * 2008-12-16 2010-06-23 F. Hoffmann-Roche AG Compositions et procédés pour compléter des puits souterrains
US8271230B2 (en) 2008-12-16 2012-09-18 Roche Molecular Systems, Inc. System and methods for monitoring a thermoelectric heating and cooling device

Also Published As

Publication number Publication date
EP1127619A3 (fr) 2001-09-05
EP1127619A2 (fr) 2001-08-29

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EP1127619B1 (fr) Dispositif d'un thermocycleur pour réactions d'amplification par polymèrase
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