EP1093463A1

EP1093463A1 - A method for preparing a compound with growth hormone releasing properties

Info

Publication number: EP1093463A1
Application number: EP99955478A
Authority: EP
Inventors: Claus Ulrik Jessen
Original assignee: Novo Nordisk AS
Current assignee: Novo Nordisk AS
Priority date: 1998-06-09
Filing date: 1999-06-09
Publication date: 2001-04-25
Also published as: AU4256799A; JP4574849B2; JP2002517514A; WO1999064456A1

Abstract

A method for preparing a compound of formula (I) or a salt thereof.

Description

A METHOD FOR PREPARING A COMPOUND WITH GROWTH HORMONE RELEASING PROPERTIES

FIELD OF INVENTION

The present invention relates to a process for preparing a compound of formula I, a GH secretagogue which can be used i.a. in treating medical disorders resulting from a deficiency in growth hormone.

BACKGROUND OF THE INVENTION

Growth hormone is a hormone which stimulates growth of all tissues capable of growing. In addition, growth hormone is known to have a number of effects on metabolic processes, e.g., stimulation of protein synthesis and free fatty acid mobilisation and to cause a switch in energy metabolism from carbohydrate to fatty acid metabolism. Deficiency in growth hormone can result in a number of severe medical disorders, e.g., dwarfism.

Growth hormone is released from the pituitary. The release is under tight control of a number of hormones and neurotransmitters either directly or indirectly. Growth hormone release can be stimulated by growth hormone releasing hormone (GHRH) and inhibited by somatostatin. In both cases the hormones are released from the hypothalamus but their action is mediated primarily via specific receptors located in the pituitary. Other compounds which stimulate the release of growth hormone from the pituitary have also been described. For example arginine, L-3,4-dihydroxyphenylalanine (L-Dopa), glucagon, vasopressin, PACAP (pituitary adenylyl cyclase activating peptide), muscarinic receptor agonists and a synthetic hexapeptide, GHRP (growth hormone releasing peptide) release endogenous growth hormone either by a direct effect on the pituitary or by affecting the release of GHRH and/or somatostatin from the hypothalamus.

In disorders or conditions where increased levels of growth hormone is desired, the protein nature of growth hormone makes anything but parenteral administration non-viable. Furthermore, other directly acting natural secretagogues, e.g., GHRH and PACAP, are longer polypeptides for which reason parenteral administration is preferred. WO 97/23508 discloses a method for preparing the compound of formula I. This method is very expensive and makes use of reagents which are adverse to the environment.

It is an object of the present invention to provide a novel method for preparing a compound of formula I which method results in high yields and high purity. Moreover the present method is reproducible and more economic, and is suitable for large scale production.

SUMMARY OF THE INVENTION

In accordance with the present invention there is provided a novel and improved method for preparing a compound of formula I

or a salt thereof, comprising a) treating a compound of formula II

NHP O

. _0H (ID

or a salt thereof, wherein P is a protecting group, with an agent capable of forming an amide or ester or mixed carbonic anhydride or anhydride or acidhalide, in a solvent selected from an organic solvent or mixture of organic solvents or a mixture of organic solvent(s) and water, thereby producing and isolating a compound of formula III

NHP O

or a salt thereof, wherein R together with the carbonyl moiety is an amide or ester or mixed carbonic anhydride or anhydride or acidhalide, b) treating the isolated compound of formula III, or a salt thereof, with a compound of formula (IV)

or a salt thereof, in a solvent selected from an organic solvent or mixture of organic solvents or a mixture of organic solvent(s) and water, thereby producing a compound of formula (V)

or a salt thereof, which is then deprotected in a conventional manner, to obtain the compound of formula (I) or a salt thereof.

DESCRIPTION OF THE INVENTION

Accordingly, the present invention relates to a method for preparing a compound of formula

or a salt thereof, comprising a) treating a compound of formula II

NHP O

OH <"> or a salt thereof, wherein P is a protecting group, with an agent capable of forming an amide or ester or mixed carbonic anhydride or anhydride or acidhalide, in a solvent selected from an organic solvent or mixture of organic solvents or a mixture of organic solvent(s) and water, thereby producing and isolating a compound of formula III

NHP O

^■R ^(l"> or a salt thereof, wherein R together with the carbonyl moiety is an amide or ester or mixed carbonic anhydride or anhydride or acidhalide, b) treating the isolated compound of formula III, or a salt thereof, with a compound of formula (IV)

In one embodiment of the present method P is a group of formula -C(=O)-C_1-12alkyl, -C(=O)- O-C.,.₁₂alkyl, -C(=O)-C_1-12alkenyl or -C^OJ-O-C_L^alkenyl, optionally substituted with one or more halogen, C^alkyl, hetaryl, aryl or fused-ring aromatic system. P is preferably selected from -C(=O)H, Troc, Boc, and Fmoc. In one embodiment P is Troc. In a second embodiment P is Boc. In a third embodiment P is Fmoc. In a most preferred embodiment P is Troc.

In another embodiment of the present method the agent capable of forming an amide or ester or mixed carbonic anhydride or anhydride or acidhalide is selected from benzotriazole, isobutyl chlorocarbonate, DHOBt, HOBt, HOSu, and HOAt. In a most preferred embodiment the agent capable of forming an amide or ester or mixed carbonic anhydride or anhydride or acidhalide is DHOBt.

Furthermore the solvent in step b of the present method is preferably a mixture of an organic solvent and water, preferably an ester and water, such as C₁.₆alkyl-C(=0)-0-C₁.₆alkyl, e.g. ethylacetate and water.

The de-protection is usually carried out by acidic, basic, oxidative or reductive cleavage, eg. when the protection group is Troc then reductive cleavage is carried out with Zn and acetic acid.

In a preferred embodiment of the present method P is Troc and the agent capable of forming an amide or ester or mixed carbonic anhydride or anhydride or acidhalide is DHOBt.

In another aspect the present invention relates to a compound of formula III

NHP O

(iιι>

or a salt thereof, wherein R together with the carbonyl moiety is an amide or ester or mixed carbonic anhydride or anhydride or acidhalide, and P is a protecting group. In one embodi- ment P is a group of formula -C(=0)-C₁.₁₂alkyl, -C(=0)-0-C_1-12alkyl, -C(=0)-C₁.₁₂alkenyl or - C(=0)-0-C₁.₁₂alkenyl, optionally substituted with one or more halogen, C_1-6alkyl, hetaryl, aryl or fused-ring aromatic system. P is preferably selected from -C(=O)H, Troc, Boc, and Fmoc. In one embodiment P is Troc. In a second embodiment P is Boc. In a third embodiment P is Fmoc. In a most preferred embodiment P is Troc. In a further embodiment the agent capable of forming an amide or ester or mixed carbonic anhydride or anhydride or acidhalide is selected from benzotriazole, isobutyl chlorocarbonate, DHOBt, HOBt, HOSu, and HOAt, preferably DHOBt. In a most preferred embodiment P is Troc and said amide or ester or mixed carbonic anhydride or anhydride or acidhalide is obtained by reacting DHOBt with the car- boxylic acid moiety in the compound of formula II. In a further aspect the present invention relates to a compound of formula V

or a salt thereof, wherein P is Troc or Fmoc. In one embodiment the compound is

The compounds of the present invention, i.e. formula I, II, III, IV and V may optionally be on a salt form, such as a pharmaceutically acceptable salt form e.g. the pharmaceutically acceptable acid addition salts of compounds of formula I, II, III, IV and V, which include those prepared by reacting the compound of formula I, II, III, IV and V with an inorganic or organic acid such as hydrochloric, hydrobromic, sulfuric, acetic, phosphoric, lactic, malic, maleic, mandelic phthalic, citric, glutaric, gluconic, methanesulfonic, salicylic, succinic, tartaric, toluenesulfonic, trifluoracetic, sulfamic or fumaric acid and/or water. Scheme 1

NHTroc

The process according to the present invention is illustrated in above scheme 1 and should in no way be interpreted as limiting the invention in any way. Thus, the above use of specific compounds are only for illustrative purposes.

The compound 1 is a commercially available compound or may be prepared from known starting materials by conventional reaction steps, for instance as illustrated in example 1 , wherein 5-(N-Boc)-Amino-5-methyl-hex-(2E)-enoic acid disclosed in e.g. WO 97/23508 is converted to 5-(N-Troc)-Amino-5-methyl-hex-(2E)-enoic acid. It is possible to start from 5-(N- Boc)-Amino-5-methyl-hex-(2E)-enoic acid and introduce a suitable protecting group, such as a group of formula -C(=O)-C₁.₁₂alkyl, -C(=O)-0-C_1-12alkyl, -C(=O)-C₁.₁₂alkenyl or -C(=O)-O-C_1- ₁₂alkenyl, optionally substituted with one or more halogen, C_1-6alkyl, hetaryl, aryl or fused-ring aromatic system, e.g. a protecting group selected from -C(=0)H, trichloroethyloxycarbonyl (Troc), tert-butyloxycarbonyl (Boc), and fluorenylmethyloxycarbonyl (Fmoc), preferably Troc, using a similar procedure as described in example 1 herein. Compound 1 is then reacted with an agent capable of forming an amide or ester or mixed carbonic anhydride or anhy- dride or acidhalide, such as benzotriazole, isobutyl chlorocarbonate, 3,4-Dihydro-3-hydroxy- 4-0x0-1 ,2,3-benzotriazine (DHOBt), N-hydroxy benzotriazol (HOBt), N-hydroxy succinimid (HOSu), and 1-hydroxy-7-azabenzotriazole (HOAt), preferably DHOBt, in a solvent selected from an organic solvent or mixture of organic solvents or a mixture of organic solvent(s) and water, such as ethylacetate, thereby producing the compound 2, which is isolated in a conventional manner, e.g. as described in connection with example 2 herein. The isolated compound 2, which is very crystallinic and thus easy to handle, is treated with compound 3, either as the free amine or as a salt, e.g. the HCI salt, in a solvent selected from an organic solvent or mixture of organic solvents or a mixture of organic solvent(s) and water, preferably a mixture of an organic solvent and water, such as an ether and water, e.g. THF and water, preferably an ester and water, such as C₁.₆alkyl-C(=0)-0-C₁.₆alkyl, e.g. ethylacetate and water, thereby producing compound 4, which is then deprotected in a conventional manner, such as by reductive cleavage with an organic acid, such as acetic acid and Zn, or the like, to obtain the compound 5, which is the compound of formula I. The compound 3 may be obtained as described in WO 97/23508.

In the above structural formulas and throughout the present specification, the following terms have the indicated meanings:

The C_1-12-alkyl, C_1j3-alkyl, or C_M-alkyl groups specified herein are intended to include those al- kyl or alkylene groups of the designated length in either a linear or branched or cyclic configuration. Examples of linear alkyl are methyl, ethyl, propyl, butyl, pentyl, hexyl and heptyl. Examples of branched alkyl are isopropyl, sec-butyl, tert-butyl, isopentyl, isohexyl and isoheptyl. Examples of cyclic alkyl are C^-cycloalkyl such as cyclopropyl, cyclobutyl, cyclopentyl, cyclo- hexyl and cycloheptyl. The C_1-6-alkoxy groups specified herein are intended to include an oxygen atom connected to a C_1-6-alkyl as defined above. Examples are methoxy, ethoxy, isopro- pyloxy, sec-butyloxy, tert-butyloxy, cyclopropyloxy, cyclobutyloxy, etc.

The term "aryl" is intended to include monovalent carbocyclic aromatic ring moieties, being ei- ther monocyclic, bicyclic or polycyclic, e.g. selected from the group consisting of phenyi and naphthyl, optionally substituted with one or more C_1-6-alkyl, C_1-6-alkoxy, halogen, amino or aryl. Such aryl groups are described in Morrison and Boyd "Organic Chemistry", 4. Ed. The term "hetaryl" is intended to include monovalent heterocyclic aromatic ring moieties, being either monocyclic, bicyclic or polycyclic, e.g. selected from the group consisting of pyridyl, 1-H- tetrazol-5-yl, thiazolyl, imidazolyl, indolyl, pyrimidinyl, thiadiazolyl, pyrazolyl, oxazolyl, isoxa- zolyl, oxadiazolyl, thienyl, quinolinyl, pyrazinyl, or isothiazolyl, optionally substituted by one or more C^-alkyl, C_1-6-alkoxy, halogen, amino or aryl. Such hetaryl groups are described in Morrison and Boyd "Organic Chemistry", 4. Ed.

The term "fused-ring aromatic system" is intended to include monovalent aromatic ring moieties, being either bicyclic or polycyclic hydrocarbons, such as a system selected from the group consisting of fluorenes, e.g. flourenyl. Such fused-ring aromatic systems are described in Morrison and Boyd "Organic Chemistry", 4. Ed.

The term "halogen" is intended to include chlorine (Cl), fluorine (F), bromine (Br) and iodine (I).

The term "Protecting group" is intended to include any group which protect the amino group when the carboxylic group in the compound of formula II is subjected to functional derivati- zation, and which is easy to remove afterwards by cleavage. Such protecting groups are described in "Protective groups in organic chemistry", 2. Ed, Greene.T.W.; Wuts.P.G.M., John Wiley&Sons.lnc. 1991 ; and "The Peptides, Analysis, Synthesis, Biology", vol 3 "Protection of Functional Groups in Peptide synthesis", Gross.E.; Meienhofer, J.; Academic Press. A suitable protecting group is a group of formula -C(=0)-C₁.₁₂alkyl, -C(=0)-O-C_1-12alkyl, -C(=O)-C₁. ₁₂alkenyl or -C(=0)-0-C_1-12alkenyl, optionally substituted with one or more halogen, C_halky!, hetaryl, aryl or fused-ring aromatic system, e.g. Troc, Boc, and Fmoc.

The term "agent capable of forming an amide or ester or mixed carbonic anhydride or anhydride or acidhalide" is intended to include such agents which activates the compound by forming a functional derivative which may be used in acylation. Such agents are described in "The Peptides, Analysis, Synthesis, Biology", vol 1 "Major Methods of Bond Formation", Gross.E.; Meienhofer, J.; Academic Press, 1981. Suitable agents are selected from benzotri- azoie, isobutyl chlorocarbonate, DHOBt, HOBt, HOSu, and HOAt.

The term "de-protection" is intended to include acidic, basic, oxidative or reductive cleavage as described in "The Peptides, Analysis, Synthesis, Biology", vol 1 "Major Methods of Bond Formation", Gross.E.; Meienhofer, J.; Academic Press, 1981. When, for instance, the protection group is Troc then reductive cleavage is carried out with Zn and acetic acid.

The compounds of formula I when used for the intended purpose of releasing endogenous growth hormone may be administered in pharmaceutically acceptable acid addition salt form or, where appropriate, as a alkali metal or alkaline earth metal or lower alkylammonium salt. Such salt forms are believed to exhibit approximately the same order of activity as the free base forms.

The compounds of the general formula I possess the ability to release endogenous growth hormone in vivo as mentioned in WO 97/23508. The compounds may therefore be used in the treatment of conditions which require increased plasma growth hormone levels such as in growth hormone deficient humans or in elderly patients or livestock.

To those skilled in the art, it is well known that the current and potential uses of growth hormone in humans are varied and multitudinous. Thus, compounds of formula I can be administered for purposes stimulating release of growth hormone from the pituitary and would then have similar effects or uses as growth hormone itself. Compounds of formula I are useful for stimulation of growth hormone release in the elderly, prevention of catabolic side effects of glu- cocorticoids, prevention and treatment of osteoporosis, treatment of chronic fatigue syndrom (CFS), treatment of acute fatigue syndrom and muscle loss following election surgery, stimulation of the immune system, acceleration of wound healing, accelerating bone fracture repair, accelerating complicated fractures, e.g. disctraction osteogenesis, treatment of wasting secondary to fractures, treatment of growth retardation, treating growth retardation resulting from re- nal failure or insufficiency, treatment of cardiomyopathy, treatment of wasting in connection with chronic liver disease, treatment of thrombocytopenia, treatment of growth retardation in connection with Crohn's disease, treatment of short bowel syndrome, treatment of wasting in connection with chronic obstructive pulmonary disease (COPD), treatment of complications associated with transplantation, treatment of physiological short stature including growth hor- mone deficient children and short stature associated with chronic illness, treatment of obesity and growth retardation associated with obesity, treatment of anorexia, treatment of growth retardation associated with the Prader-Willi syndrome and Turner's syndrome; increasing the growth rate of a patient having partial growth hormone insensitive syndrome, accelerating the recovery and reducing hospitalization of burn patients; treatment of intrauterine growth retarda- tion, skeletal dysplasia, hypercortisolism and Cushing's syndrome; induction of pulsatile growth hormone release; replacement of growth hormone in stressed patients, treatment of osteo- chondrodysplasias, Noonan's syndrome, schizophrenia, depressions, Alzheimer's disease, delayed wound healing and psychosocial deprivation, treatment of catabolism in connection with pulmonary dysfunction and ventilator dependency, treatment of cardiac failure or related vascular dysfunction, treatment of impaired cardiac function, treatment or prevention of myo- cardial infarction, lowering blood pressure, protection against ventricular dysfunction or prevention of reperfusion events, treatment of adults in chronic dialysis, attenuation of protein cata- bolic responses after major surgery, reducing cachexia and protein loss due to chronic illness such as cancer or AIDS; treatment of hyperinsulinemia including nesidioblastosis, adjuvant treatment for ovulation induction; stimulation of thymic development and prevention of the age-related decline of thymic function, treatment of immunosuppressed patients, treatment or sarcopenia, treatment of wasting in connection with AIDS, improvement in muscle strength, mobility, maintenance of skin thickness, treatment of metabolic homeostasis and renal homeo- stasis in the frail elderly, stimulation of osteoblasts, bone remodelling and cartilage growth, regulation of food intake, stimulation of the immune system in companion animals and treatment of disorders of aging in companion animals, promoting growth in livestock and stimulation of wool growth in sheep, increasing milk production in livestock, treatment of metabolic syndrome (syndrome X), treatment of insulin resistance, including NIDDM, in mammals, e.g. hu- mans, treatment of insulin resistance in the heart, improvement of sleep quality and correction of the relative hyposomatotropism of senescence due to high increase in REM sleep and a decrease in REM latency, treatment of hypothermia, treatment of frailty associated with aging, treatment of congestive heart failure, treatment of hip fractures, treatment of immune deficiency in individuals with a depressed T4/T8 cell ratio, treatment of muscular atrophy, treat- ment of musculoskeletal impairment in elderly, enhancing the activity of protein kinase B (PKB), improvement of the overall pulmonary function, and treatment of sleep disorders. Treatment is also intended to include prophylactic treatment.

For the above indications the dosage will vary depending on the compound of formula I em- ployed, on the mode of administration and on the therapy desired. The dosage of the compounds according to this invention is suitably 0.01-500 mg/day, e.g. from about 5 to about 50 mg, such as about 10 mg per dose, when administered to patients, e.g. humans, as a drug. However, generally dosage levels between 0.0001 and 100 mg/kg body weight daily are administered to patients and animals to obtain effective release of endogenous growth hormone. Morever the compounds of formula I have no or substantially no side-effects, when administered in the above dosage levels, such side-effects being e.g. release of LH, FSH, TSH, ACTH, vasopressin, oxytocin, cortisol and/or prolactin. Usually, dosage forms suitable for oral, nasal, pulmonal or transdermal administration comprise from about 0.0001 mg to about 100 mg, pref- erably from about 0.001 mg to about 50 mg of the compounds of formula I admixed with a pharmaceutically acceptable carrier or diluent.

Optionally, a pharmaceutical composition comprising the compound of formula I prepared by the method of the invention may be combined with one or more compounds exhibiting a differ- ent activity, e.g., an antibiotic or other pharmacologically active material.

The route of administration may be any route which effectively transports the active compound to the appropriate or desired site of action, such as oral, nasal, pulmonary, transdermal or parenteral, the oral route being preferred.

Apart from the pharmaceutical use of the compounds of formula I, they may be useful in vitro tools for investigating the regulation of growth hormone release.

Compounds of formula I may also be useful in vivo tools for evaluating the growth hormone releasing capability of the pituitary. For example, serum samples taken before and after administration of these compounds to humans can be assayed for growth hormone. Comparison of the growth hormone in each serum sample would directly determine the ability of the patients pituitary to release growth hormone.

Compounds of formula I may be administered to commercially important animals to increase their rate and extent of growth, and to increase milk production.

A further use of growth hormone secretagogue compounds of formula I is in combination with other secretagogues such as GHRP (2 or 6), GHRH and its analogues, growth hormone and its analogues or somatomedins including IGF-1 and IGF-2.

Any novel feature or combination of features described herein is considered essential to this invention. EXAMPLES:

The process for preparing compounds of formula I is further illustrated in the following examples, which however, are not to be construed as limiting.

The structures of the compounds are confirmed by either elemental analysis (MA) nuclear magnetic resonance (NMR) or mass spectrometry (MS). NMR shifts (d) are given in parts per million (ppm) and only selected peaks are given, mp is melting point and is given in °C. Column chromatography was carried out using the technique described by W.C. Still et al, J. Org. Chem. 1978, 43, 2923-2925 on silica gel 60. Compounds used as starting materials are either known compounds or compounds which can readily be prepared by methods known per se.

Example 1 Synthesis of 5-(N-Troc)-amino-5-methyl-hex-2E-enoic acid

104g (0,43mol) 5-(N-Boc)-Amino-5-methyl-hex-(2E)-enoic acid is dissolved in 800ml of ethylacetate (EtOAc). The mixture is cooled to 0-5°C on an ice bath and HCI gas is bubbled to the mixture while stirring. Precipitation occurs after 20min. The temperature rises to max15°C via control of HCI addition. App. 92g of HCI gas is used to complete the reaction. The reaction is followed via HPLC.

The next day the mixture is evaporated to halve volume and stripped with 2x100ml EtOAc in order to remove HCI. The mixture is kept at 0-5°C for 4h. The resulting precipitate is isolated and dried at 30°C in the vacuum to constant yield. The yield of 5-Amino-5-methyl-hex-(2E)- enoic acid, HCI is 73g (95%). The HCI salt is dissolved in 730 ml NaHC0₃/NaOH buffer, pH 11, and 730 ml 2-PrOH, pH is approx. 9,5. Cooled to0-5°C.

Succinimidyl-2,2,2-trichloroethylcarbonate (TrocOSu) is added in 3 portions over 1.5 hours. pH is maintained at 9.5-10 with 2M NaOH (approx. 75ml). Precipitation occurs. The mixture is stirred overnight at 0-5°C to complete the reaction. The reaction mixture (pH 9.1) is fil- tered. The 2-propanol (2-PrOH) content in the filtrate is distilled off under vacuum (30°C). pH is adjusted to 9.5 with 2M NaOH. 500ml methyl-tert-butylether (MTBE) are added. 3 phases occur. The two lower phases which contains the product are isolated. The organic MTBE phase is extracted with 300ml water (pH 10, adjusted with aq. NaOH). The aqueous phases are combined and 500ml of MTBE are added. The mixture is acidified to pH 2 with 4M HCI at ambient temperature. Evolution of C0₂ gas. The organic phase is isolated and the water phase extracted with 500ml MTBE. The combined organic phases are dried. Evaporation of solvent yields 131g of 5-(N-Troc)-amino-5-methyl-hex-(2E)-enoic acid (100%). It is isolated as an oil which slowly crystallises.

Example 2

5-ιN-Trichloroethyl-oxycarbonyl)-amino-5-methyl-hex-(2E)-en-(3,4-Dihydro-3-hydroxy-4-oxo- 1.2.3-benzotriazine) acid ester 102g of 5-(N-Trichloroethyloxycarbonyl)-amino-5-methyl-hex-(2E)-enoic acid and 66g of 3,4- Dihydro-3-hydroxy-4-oxo-1 ,2,3-benzotriazine (DHOBt) are added to 1000ml EtOAc while stirring. The mixture is cooled to 0 to -5°C and 52g dicyclohexyl carbodiimide (DCC) dissolved in 300ml EtOAc is added over 1 hour under nitrogen atmosphere. The solution is stirred overnight at 0 to -5°C to complete the reaction. The solution is warmed up to ambient tem- perature and filtered . The EtOAc content in the filtrate is distilled off under vacuum (30°C) and replaced with 2-PrOH. Residual EtOAc is stripped off with 2-PrOH (2x 200ml). The raw product is re-crystallised in 950ml of 2-PrOH. Crystallisation starts at 70°C. The mixture is slowly cooled to 0-5°C and stirred overnight at 0-5°C. The mixture is filtered and washed with 2-PrOH. The filter cake is dried under vacuum to constant weight. The yield is 93g of 5- (N-Trichloroethyl-oxycarbonyl)-amino-5-methyl-hex-2E-en-(3,4-Dihydro-3-hydroxy-4-oxo- 1 ,2,3-benzotriazine) acid ester (63%). White crystals.

Example 3

Synthesis of 5-(N-Trichloroethyloxycarbonyl)amino-5-methylhex-(2E)-enoic acid N-methyl-N- ( ( 1 RV-1 -( N-methyl-N-(f 1 R 1 -(methylcarbamoyl 2-phenylethyπcarbamoyn-2-(2- naphthy ethyhamide

A 2 L reactor is equipped with a mechanical stirrer, thermometer, heating facility and N₂-inlet. 80,2g N-Methyl-N-((1 R)-1-(N-methyl-N-((1 R)-1-(methylcarbamoyl)-2-phenylethyl)carbamoyl)- 2-(2-naphthyl)ethyl)amide, HCI is suspended in 540ml EtOAc at 20-25°C. 18,6g N-methyl morpholine (NMM) and thereafter 540ml water is added. N-Methyl-N-((1 R)-1-(N-methyl-N- ((1 R)-1 -(methylcarbamoyl)-2-phenylethyl)carbamoyl)-2-(2-naphthyl)ethyl)amide, HCI slowly dissolves. After most of N-Methyl-N-((1 R)-1-(N-methyl-N-((1R)-1-(methylcarbamoyl)-2- phenylethyl)carbamoyl)-2-(2-naphthyl)ethyl)amide, HCI is dissolved 5-(N-Trichloroethyl- oxycarbonyl)-amino-5-methyl-hex-2E-en-(3,4-Dihydro-3-hydroxy-4-oxo-1 ,2,3-benzotriazine) acid ester is put into the reactor and the mixture is heated to 50-55°C. The temperature is retained at 50-55°C for 24-48h in order to complete the reaction. After cooling 200ml EtOAc is added and the organic layer isolated. The organic layer is washed with 1x 500ml 0,5M KHSO₄, 1x 500ml sat.NaHCO₃ and 1x 300ml water. Thereafter the organic phase is dried over MgSO₄ and filtered leaving 146g of title compound (>100%, contains EtOAc) oil/foam after evaporation.

The utilisation of 37,2g instead of 18,6g NMM enhances significantly the reactivity and the reaction is finished in less than 24 hours.

Example 4

Synthesis of compound of formula I

A 1 L reactor is equipped with a mechanical stirrer, thermometer, heating/cooling facility, dropping funnel and N₂-inlet. 35,3g activated Zn dust (<60μm) is suspended in 162ml acetic acid. 5-(N-Trichloroethyloxycarbonyl)amino-5-methylhex-(2E)-enoic acid N-methyl-N-((1R)-1- (N-methyl-N-((1 R)-1-(methylcarbamoyl)-2-phenylethyl)carbamoyl)-2-(2-naphthyl)ethyl)amide dissolved in 162ml EtOAc is slowly added over 3h. Heat and gas is developed, especially in the beginning. The temperature is kept at 30-35°C for 18-24h in order to complete the reac- tion. Next day the mixture is cooled to 20-25°C and 200ml EtOAc and 600ml water are added. H₂ is developed. The reaction mixture is filtered and the water phase isolated. The organic layer is extracted with 1x 500ml and 1x 100ml 0,01 M HCI. 800ml EtOAc is added to the combined water phases and the pH is adjusted to 9 with 400ml 25% NH₃ in water. The temperature is kept at 15-25°C. The organic layer is isolated and the water phase extracted with 400ml EtOAc. The combined organic phase is washed with 1x500ml and 1x 250ml 1% NH₃ in water, dried over MgSO₄, filtered and evaporated to dryness leaving 94g of the compound of formula I (99%, contains EtOAc). Off white foam/oil.

Example 5 Synthesis of compound of formula I using Fmoc as protection group

The synthesis of the compound of formula I could be performed using the same methods as described in examples 1-4 but with Fmoc as projection group instead of Troc. In that case the steps would be synthesis of 5-(N-Fmoc)-amino-5-methyl-hex-2E-enoic acid, followed by synthesis of 5-(N-Fluorenylmethyloxycarbonyl)-amino-5-methyl-hex-(2E)-en-(3,4- Dihydro-3-hydroxy-4-oxo-1 ,2,3-benzotriazine) acid ester, followed by synthesis of 5-(N- Fluo- renylmethyloxycarbonyl)amino-5-methylhex-(2E)-enoic acid N-methyl-N-((1 R)-1-(N-methyl- N-((1 R)-1 -(methylcarbamoyl)-2-phenylethyl)carbamoyl)-2-(2-naphthyl)ethyl)amide using 18,6 g of NMM and keeping pH below 7. This compound is deprotected to the compound of for- mula I.

Reference example

Preparation of the compound of formula I as described in WO97/23508.

3-Hydroxy-1 , 1 -dimethylpropylcarbamic acid tert-butyl ester:

Step A: At 0 °C, ethyl chloroformate (1.10 mL, 11.5 mmol) was given dropwise to a solution of 3-tert-butoxycarbonylamino-3-methylbutanoic acid (2.50 g, 11.5 mmol) and triethylamine (1.92 mL, 13.8 mmol) in tetrahydrofuran (10 mL). The solution was stirred for 40 min at 0 °C. The formed precipitate was filtered off and washed with tetrahydrofuran (20 mL). The liquid was immediately cooled to 0 °C. A 2M solution of lithium boronhydride in tetrahydrofuran (14.4 mL, 28.8 mmol) was added dropwise. The solution was stirred at 0 °C for 2 h, and then warmed to room temperature, over a period of 4 h. It was cooled to 0 °C. Methanol (5 mL) was added carefully. 1N Hydrochloric acid (100 mL) was added. The solution was extracted with ethyl acetate (2 x 100 mL, 3 x 50 mL). The combined organic layers were washed with saturated sodium hydrogen carbonate solution (100 mL) and dried over magnesium sulfate. The solvent was removed in vacuo. The crude product was chromatographed on silica (110 g) with ethyl acetate/heptane 1 :2 to give 1.84 g of 3-hydroxy-1 ,1-dimethylpropylcarbamic acid tert-butyl ester.

3-(tert-Butoxycarbonylamino)-3-methylbutanal:

Step B: DMSO (1.22 mL, 17.2 mmol) was added to a solution of oxalyl chloride (1.1 mL, 12.9 mmol) at -78 °C in dichloromethane (15 mL). The mixture was stirred for 15 min at -78 °C. A solution of 3-hydroxy-1 ,1-dimethylpropylcarbamic acid tert-butyl ester (1.75 g, 8.6 mmol) in dichloromethane (10 mL) was added dropwise over a period of 15 min. The solution was stirred at -78 °C for another 15 min. Triethylamine (6.0 mL, 43 mmol) was added. The solution was stirred at -78 °C for 5 min and then warmed to room temperature. The solution was diluted with dichloromethane (100 mL) and extracted with 1N hydrochloric acid (100 mL). The aqueous phase was extracted with dichloromethane (50 mL). The combined organic layers were washed with saturated sodium hydrogen carbonate solution (100 mL) and dried over magnesium sulfate. The solvent was removed in vacuo. The crude product was purified by column chromatography on silica (140 g) with ethyl acetate/heptane (1 :3) to give 1.10 g of 3- (tert-butoxycarbonylamino)-3-methylbutanal.

Ethyl (2E)-5-(tert-Butoxycarbonylamino)-5-methylhex-2-enoate:

Step C: Triethylphoshonoacetate (1.96 mL, 9.8 mmol) was dissolved in tetrahydrofuran (30 mL). Potassium tert-butoxide (1.10 g, 9.8 mmol) was added. The solution was stirred for 40 min at room temperature. A solution of 3-(tert-butoxycarbonylamino)-3-methylbutanal (1.10 g, 5.5 mmol) in Tetrahydrofuran (6 mL) was added. The solution was stirred at room temperature, for 75 min. It was diluted with ethyl acetate (100 mL) and 1N hydrochloric acid (100 L). The phases were separated. The aqueous phase was extracted with ethyl acetate (2 x 50 mL). The combined organic phases were washed with saturated sodium hydrogen carbonate solution (60 mL) and dried over magnesium sulfate. The solvent was removed in vacuo. The crude product was purified by column chromatography on silica (90 g) with ethyl acetate/hepatane (1 :4) to give 1.27 g of ethyl (2E)-5-(tert-butoxycarbonylamino)-5-methylhex-2-enoate. (2E)-5-(tert-Butoxycarbonylamino)-5-methylhex-2-enoic acid:

Step D: Ethyl (2E)-5-(tert-butoxycarbonylamino)-5-methylhex-2-enoate (1.233 g, 4.54 mmol) was dissolved in dioxane (20 mL). Lithium hydroxide (0.120 g, 5.00 mmol) was added as a solid. Water (10 mL) was added, until a clear solution was reached. The solution was stirred 16 h at room temperature. The solution was diluted with water (70 L) and was extracted with tert-butyl methyl ether (2 x 100 mL). The aqueous phase was acidified with 1N sodium hydrogensulfate solution (pH = 1) and was extracted with tert-butylmethylether (3 x 70 mL). The organic phases were combined and dried over magnesium sulfate. The solvent was removed in vacuo to give 1.05 g of (2E)-5-(tert-butoxycarbonylamino)-5-methylhex-2-enoic acid. The crude product was used for further syntheses.

N-Methyl-N-((R)-1-(methylcarbamoyl)-2-phenylethyl)carbamic acid tert-butyl ester:

Step E: N-Tert-butoxycarbonyl-N-methyl-D-phenylalanine (1.22 g, 4.4 mmol), 1- hydroxybenzotriazole hydrate(0.59 g, 4.4 mmol) and 1-ethyl-3-(3-dimethyl- aminopropyl)carbodiimid hydrochloride (0.88 g, 4.6 mmol) were dissolved in N,N- dimethylformamide (25 mL) and stirred for 30 min. Methylamine (0.51 g of a 40% solution in methanol, 6.6 mmol) was added and the mixture was stirred overnight. Methylene chloride (80 mL) and water (100 mL) were added and the phases were separated. The organic phase was washed with sodium hydroxide (20 mL, 1N), sodium hydrogensulfate (50 mL, 10 %) and water (50 mL). The organic phase was dried (magnesium sulfate) and the solvent removed in vacuo to afford 1.39 g of N-methyl-N-((R)1-(methylcarbamoyl)-2-phenylethyl)carbamic acid tert-butyl ester.

(R)-N-Methyl-2-methylamino-3-phenylpropionamide:

Step F: N-Methyl-N-((R)1-(methylcarbamoyl)-2-phenylethyl)carbamic acid tert-butyl ester (1.39 g, 7.23mmol) was dissolved in a mixture of trifluoroacetic acid (5 mL) and methylene chloride (10 mL) and stirred for 45 min. The volatiles were removed in vacuo and the residue was stirred with a mixture of ethyl acetate (100 mL) and water (100 mL). Sodium hydrogen carbonate (50 mL, saturated) was added and the phases were separated. The organic phase was dried (magnesium sulfate) and the solvent removed in vacuo to afford 330 mg of (R)-N- methyl-2-methylamino-3-phenylpropionamide.

N-Methyl-N-{(1 R)-1 -(N-methyl-N-((1 R)-1 -(methylcarbamoyl)-2-phenylethyl)carbamoyl)-2-(2- naphthyl)ethyl}carbamic acid tert-butyl ester:

Step G: (R)-Tert-butoxycarbonyl-N-methylamino-3-(2-naphthyl)propionic acid (548 mg, 1.66 mmol) was dissolved in methylene chloride (5 mL); 1-hydroxy-7-azabenzotriazole (227 mg, 1,66 mmol) was added along with N,N-dimethylformamide (2 mL). 1-Ethyl-3-(3- dimethylaminopropyl)carbodiimide hydrochloride (351 mg, 1.83 mmol) was added and the solution was stirred for 15 min. (R)-N-Methyl-2-methylamino-3-phenylpropionamide (320 mg, 1.66 mmol) dissolved in methylene chloride (4 mL) and diisopropylethylamine (0.28 mL, 1.66 mmol) were added and the mixture was stirred overnight. Methylene chloride (50 mL) was added and the organic phase was washed with water (100 mL), sodium hydrogensulfate (50 mL, 5%) and sodium hydrogen carbonate (50 L, saturated). The organic phase was dried (magnesium sulfate) and the solvent removed in vacuo. The residue was chromatographed (silica, 2 x 45 cm) using ethylacetate/methylene chloride (1:1) to afford 604 mg of N-methyl-N- {(1 R)-1 -(N-methyl-N-((1 R)-1 -(methylcarbamoyl)-2-phenylethyl)carbamoyl)-2-(2-naphthyl)- ethyl}carbamic acid tert-butyl ester.

(2R)-N-Methyl-2-methylamino-N-((1R)-1-(methylcarbamoyl)-2-phenylethyl)-3-(2- naphthyl)propionamide:

Step H: N-Methyl-N-{(1 R)-1 -(N-methyl-N-((1 R)-1 -(methylcarbamoyl)-2-phenylethyl)carbamoyl)- 2-(2-naphthyl)ethyl}carbamic acid tert-butyl ester (600 mg, 1.19 mmol) was stirred in trifluoroacetic acid/methylene chloride (1:1, 5 mL) for 10 min and the volatiles were removed in vacuo. The residue was stripped with diethylether (2 x 5 mL) and dissolved in methanol (2 mL) and mixed with sodium hydrogen carbonate (10 mL) and ethylacetate (15 mL). The organic phase was separated and dried (magnesium sulfate) to afford 420 mg of (2R)-N-methyl-2- methylamino-N-((1 R)-1-(methylcarbamoyl)-2-phenylethyl)-3-(2-naphthyl)propionamide. ((3E)-1 , 1 -Dimethyl-4-(N-methyl-N-((1 R)-1 -(N-methyl-N-((1 R)-1 -(methylcarbamoyl)-2- phenylethyl)carbamoyl)-2-(2-naphthyl)ethyl)carbamoyl)but-3-enyl)carbamic acid tert-butyl ester:

Step I: (2E)-5-(tert-Butyloxycarbonylamino)-5-methylhex-2-enoic acid (200 mg, 0.82 mmol), 1- hydroxy-7-azabenzotriazole (112 mg, 0.82 mmol) and 1-ethyl-3-(3-dimethylaminopropyl)- carbodiimide hydrochloride (173 mg, 0.90 mmol) were dissolved in a mixture of methylene chloride (10 mL) and N,N-dimethylformamide (1 mL) and strirred for 15 min. N-Methyl-2- methylamino-N-((1 R)-1 -(methylcarbamoyl)-2-phenylethyl)-3-(2-naphthyl)propionamide (332 mg, 0.82 mol) dissolved in methylene chloride (5 mL) and diisopropylethylamine (0.14 mL) were added and the mixture was stirred overnight under nitrogen atmosphere. The mixture was diluted with methylene chloride (50 mL), washed with water (50 mL), sodium hydrogen carbonate (30 mL, saturated), and sodium hydrogensulfate (30 mL, 5%). The phases were separated and the organic phase was dried with magnesium sulfate and evaporated in vacuo. The residue was chromatographed (silica, 2 x 40 cm) to afford 450 mg of ((3E)-1 ,1-dimethyl-4- (N-methyl-N-((1 R)-1 -(N-methyl-N-((1 R)-1 -(methylcarbamoyl)-2-phenylethyl)carbamoyl)-2-(2- naphthyl)ethyl)carbamoyl)but-3-enyl)-carbamic acid tert-butyl ester.

Step J: ((3E)-1 , 1 -Dimethyl-4-(methyl-((1 R)-1 -(methyl-((1 R)-1 -(methylcarbamoyl)-2- phenylethyl)carbamoyl)-2-(2-naphthyl)ethyl)carbamoyl)but-3-enyl)carbamic acid tert-butyl ester (403 mg, 0.63 mmol) was stirred in a mixture of trifluroacetic acdi (4mL) and methylene chloride (4 mL) for 10 min. The volatiles were removed in vacuo and the crude product was chromatograped on silica (400g) using a mixture of methylene chloride, ethanol and ammonia (25% in water) (80/18/2) as eluent. The isolated product was dissolved in 3M hydrochloric acid in ethyl acetate and evaporated, then redissolved in methylene chloride and evaporated twice to afford 140 mg of the title compound.

Claims

1. A method for preparing a compound of formula I

or a salt thereof, comprising a) treating a compound of formula II

NHP O

or a salt thereof, in a solvent selected from an organic solvent or mixture of organic solvents or a mixture of organic solvent(s) and water, thereby producing a compound of formula (V) or a salt thereof, which is then deprotected in a conventional manner, to obtain the compound of formula (I) or a salt thereof.

2. The method according to claim 1 wherein P is a group of formula -C(=O)-C_1-12alkyl, - C(=0)-0-C₁.₁₂alkyl, -C^O^C^alkenyl or -C(=0)-0-C_1-12alkenyl, optionally substituted with one or more selected from halogen, C_1-6alkyl, hetaryl, aryl or fused-ring aromatic system.

3. The method according to claim 2 wherein P is selected from -C(=0)H, Troc, Boc, and Fmoc.

4. The method according to any one of claims 1-3 wherein said agent capable of forming an amide or ester or mixed carbonic anhydride or anhydride or acidhalide is selected from ben- zotriazole, isobutyl chlorocarbonate, DHOBt, HOBt, HOSu, and HOAt.

5. The method according to claim 4 wherein said active amide or active ester forming agent or mixed carbonic anhydride is DHOBt.

6. The method according to any one of claims 1-5 wherein said solvent in step b is a mixture of an organic solvent and water.

7. A compound of formula III

NHP O

^" R ^(lll) or a salt thereof, wherein R together with the carbonyl moiety is an amide or ester or mixed carbonic anhydride or anhydride or acidhalide and P is a protecting group.

8. The compound according to claim 7 wherein P is a group of formula -C(=0)-C_1-12alkyl, - C(=O)-O-C₁.₁₂alkyl, -C(=O)-C_1-12alkenyl or -C^OJ-O-C^alkenyl, optionally substituted with one or more selected from halogen, C_1-6alkyl, hetaryl, aryl or fused-ring aromatic system.

9. The compound according to claim 8 wherein P is selected from -C(=0)H, Troc, Boc, and Fmoc.

10. The compound according to any one of claims 7-9 wherein said amide or ester or mixed carbonic anhydride or anhydride or acidhalide is obtained by reacting benzotriazole, isobutyl chlorocarbonate, DHOBt, HOBt, HOSu, or HOAt, with the carboxylic acid moiety in the compound of formula II.

11. The compound according to claim 10 wherein said amide or ester or mixed carbonic anhydride or anhydride or acidhalide is obtained by reacting DHOBt with the carboxylic acid moiety in the compound of formula II.

12. The compound according to any one of claims 7-11 wherein P is Troc and said amide or ester or mixed carbonic anhydride or anhydride or acidhalide is obtained by reacting DHOBt with the carboxylic acid moiety in the compound of formula II.

13. A compound of formula (V)

or a salt thereof, wherein P is Troc or Fmoc.