EP1071685A4

EP1071685A4 - Titanium catalyzed preparation of carbapenem intermediates

Info

Publication number: EP1071685A4
Application number: EP99916629A
Authority: EP
Inventors: Philip J Pye; Paul J Reider; Kai Rossen; Ralph P Volante
Original assignee: Merck and Co Inc
Current assignee: Merck and Co Inc
Priority date: 1998-04-16
Filing date: 1999-04-12
Publication date: 2001-07-18
Also published as: CA2328219A1; JP2002511470A; WO1999052908A1; AU745980B2; EP1071685A1; AU3490899A

Abstract

An efficient method for the synthesis of a compound of formula (2) is disclosed which comprises reacting a 4-acyl-2-azetidinone with a titanium, zirconium or hafnium enolate of a 1-hydroxy-2-butanone derivative.

Description

TITLE OF THE INVENTION TITANIUM CATALYZED PREPARATION OF CARBAPENEM INTERMEDIATES

BACKGROUND OF THE INVENTION

The present invention relates to a process for synthesizing l-β-methyl-2- hydroxymethyl carbapenem intermediates. Generally the carbapenems are substituted at the 2-position. The intermediate compounds are included as well. European applications 0330108, 0102239, 0212404, 0695753 and 0476649 disclose methods for synthesizing various antibiotic derivatives.

Many of the carbapenems are useful against gram positive microorganisms, especially methicillin resistant Staphylococcus aureus (MRSA), methicillin resistant Staphylococcus epidermidis (MRSE), and methicillin resistant coagulase negative Staphylococci (MRCNS). These antibacterials thus comprise an important contribution to therapy for treating infections caused by these difficult to control pathogens. There is an increasing need for agents effective against such pathogens (MRSA/MRCNS) which are at the same time relatively free from undesirable side effects.

SUMMARY OF THE INVENTION The invention describes a short and high yielding synthesis of protected l-β-methyl-2-hydroxymethyl substituted carbapenems as key intermediates for the synthesis of anti-MRSA carbapenem antibiotics. The synthesis involves a highly diastereoselective addition of a titanium, zirconium or hafnium enolate of a suitably protected l-hydroxy-2- butanone derivative with 4-acyl-2-azetidinone. Using this enolate, the resulting derivatized 2-azetidinone product is obtained largely as a single diastereomer rather than a mixture. Additionally, the two chiral centers which are produced are of the correct absolute stereochemical

- 1 - configuration for subsequent synthesis of l-β-methyl-2-hydroxymethyl substituted carbapenems.

In one aspect of the invention, a process of synthesizing a compound of formula 2:

R¹

X H H U

H₃C O^-YR²

O

^— NH

O

is disclosed wherein R¹ represents H or a suitable protecting group for an alcohol; R² represents a benzyl, C^ alkyl or aryl; Y represents C^_g alkyl, O, NH or S; and X represents O, NH, or S comprising reacting a compound of formula 1:

O

H,C H O Λ R

— NH

O

wherein R¹ is described above and R⁴ represents C₁.₁₅ alkyl, aryl or C₁ ar alkyl; with a compound of formula 3:

^TT^< Y^YR:

X

wherein R², X and Y are as previously defined in the presence of WZ₄ and an amine to produce a compound of formula 2, wherein W is a titanium, zirconium or hafnium metal and Z represents halo, sulfonate, alkoxy, aryloxy or combination thereof. DETAILED DESCRIPTION OF THE INVENTION

The present invention relates to a process for making protected l-β-methyl-2-hydroxymethyl substituted carbapenems which are key intermediates in the synthesis of anti-MRSA carbapenem antibiotics (such as those disclosed in USSN 08/825,786 filed on April 08, 1997, the teachings of which are hereby incorporated by reference). The intermediates can be readily coupled to a wide range of functional groups (see USSN 08/825,786). The invention is described herein in detail using the terms defined below unless otherwise specified.

The term "alkyl" refers to a monovalent alkane (hydrocarbon) derived radical containing from 1 to 10 carbon atoms unless otherwise defined. It may be straight, branched or cyclic. Preferred alkyl groups include methyl, ethyl, propyl, isopropyl, butyl, t- butyl, cyclopentyl and cyclohexyl. When substituted, alkyl groups may be substituted with up to four substituent groups, selected from R^ and R¹, as defined, at any available point of attachment. When the alkyl group is said to be substituted with an alkyl group, this is used interchangeably with "branched alkyl group".

Cycloalkyl is a species of alkyl containing from 3 to 15 carbon atoms, without alternating or resonating double bonds between carbon atoms. It may contain from 1 to 4 rings which are fused. The term "alkenyl" refers to a hydrocarbon radical straight, branched or cyclic containing from 2 to 10 carbon atoms and at least one carbon to carbon double bond. Preferred alkenyl groups include ethenyl, propenyl, butenyl and cyclohexenyl.

The term "alkynyl" refers to a hydrocarbon radical straight or branched, containing from 2 to 10 carbon atoms and at least one carbon to carbon triple bond. Preferred alkynyl groups include ethynyl, propynyl and butynyl.

Aryl refers to aromatic rings e.g., phenyl, substituted phenyl and the like as well as rings which are fused, e.g., naphthyl, phenanthrenyl and the like. An aryl group thus contains at least one ring having at least 5 atoms, with up to five such rings being present, containing up to 22 atoms therein, with alternating (resonating) double bonds between adjacent carbon atoms or suitable heteroatoms. The preferred aryl groups are phenyl, naphthyl and phenanthrenyl. Aryl groups may likewise be substituted as defined. Preferred substituted aryls include phenyl and naphthyl.

Aryl also refer to heteroaryl, which is a monocyclic aromatic hydrocarbon group having 5 or 6 ring atoms, or a poly cyclic aromatic group having 8 to 16 atoms, containing at least one heteroatom, O, S, S(O), Sθ2 or N, in which a carbon or nitrogen atom is the point of attachment, and in which one or two additional carbon atoms is optionally replaced by a heteroatom selected from O or S, and in which from 1 to 3 additional carbon atoms are optionally replaced by nitrogen heteroatoms, said heteroaryl group being optionally substituted as described herein. Examples of this type are pyrrole, pyridine, oxazole, thiazole and oxazine. Additional nitrogen atoms may be present together with the first nitrogen and oxygen or sulfur, giving, e.g., thiadiazole and the like.

As used herein, "aralkyl" is intended to mean an aryl or heteroaralkyl moiety, as defined above, attached through a C^_g alkyl linker, where alkyl is defined above. Examples of aralkyls include, but are not limited to, benzyl, naphtylmethyl, phenylpropyl, 2-pyridylmethyl, 2-imidazolylethyl, 2-quinolinylmethy, 2-imidazolylmethyl and the like.

Examples of polycyclic heteroaromatics include benzopyrans, benzofurans, benzopyrroles, benzimidazoles, benzothiazoles, quinolines, purines, isoquinolines, benzopyrimidines, dibenzofurans, dibenzothiophenes, 1,8-naphthosultams,

The term "heterocycle" (heterocyclyl) refers to a 5-16 membered cycloalkyl group (nonaromatic) with 1-4 rings, in which one of the carbon atoms in the ring is replaced by a heteroatom selected from O, S or N, and in which up to three additional carbon atoms may be replaced by heteroatoms.

The term "heteroatom" means O, S, S(O), S(0)₂ or N, selected on an independent basis.

- 4 - Halogen and "halo" refer to bromine, chlorine, fluorine and iodine.

When a group is termed "protected", such as R¹, R⁵ and the like, this means that the group is in modified form to preclude undesired side reactions at the protected site. Suitable protecting groups for the compounds of the present invention will be recognized from the present application taking into account the level of skill in the art, and with reference to standard textbooks, such as Greene, T. W. et al. Protective Groups in Organic Synthesis Wiley, New York (1991). Examples of suitable protecting groups are contained throughout the specification. In some of the compounds of the present invention, R¹ and R⁵ represent alcohol and carboxyl protecting groups, respectively. Likewise, Y may represent a protecting group for X, which in turn represents O or N. These groups are generally removable, i.e., they can be removed, if desired, by procedures which will not cause cleavage or other disruption of the remaining portions of the molecule. Such procedures include chemical and enzymatic hydrolysis, treatment with chemical reducing or oxidizing agents under mild conditions, treatment with a transition metal catalyst and a nucleophile and catalytic hydrogenation.

Examples of carboxyl protecting groups R⁵ include allyl, benzhydryl, 2-naphthylmethyl, benzyl, silyl groups such as t-butyldimethylsilyl (TBDMS), trimethylsilyl, (TMS), triethylsilyl (TES), phenacyl, p-methoxybenzyl, o-nitrobenzyl, p-methoxyphenyl, p- nitrobenzyl (pNB), 4-pyridylm ethyl and t-butyl, preferably pNB and benzyl.

Examples of suitable alcohol protecting groups R¹ include hydrogen, trialkylsilyl, diarylalkylsilyl, aryldialkylsilyl or trityl such as TMS, TES, TBDMS, alkyl carbonates such as benzyl carbonate, allyl carbonate, benzyl ether, diarylalkylsilyl, aryldialkylsilyl & trityl and the like. Preferred R¹ groups are trialkylsilyl or hydrogen.

Another aspect of the process that is of particular interest is the synthesis of a compound of formula 5:

5 -

5 c ^o-R⁵

wherein R¹ represents H or a suitable protecting group for an alcohol; R² represents a benzyl, C^ alkyl or aryl; Y represents C^₃ alkyl, O, NH or S; X represents O, NH, or S and R⁵ represents a carboxy protecting group, comprising reacting a compound of formula 2:

H.C

wherein Rl, R2, X and Y are as previously described, with an activated oxalic acid agent in the presence of a base to produce a compound of formula 5. In another aspect of the invention a process for synthesizing a compound of structural formula 6

H,C

is disclosed wherein R¹ represents H or a suitable protecting group for an alcohol; R² represents a benzyl, C_α.6 alkyl or aryl; Y represents C_{l 3} alkyl, O, NH or S; X represents O, NH, or S and R⁵ represents a carboxy protecting group, comprising reacting a compound of formula 5:

H C

O

R⁵

wherein R¹, R², R⁵, X and Y are as previously described with a phosphite or phosphonite reagent to produce a compound of formula 6.

Another aspect of the process that is of interest is the synthesis of a carbapenem compound of formula 6

wherein R¹ represents H or a suitable protecting group for an alcohol; R² represents a benzyl, C^ alkyl or aryl; Y represents C^g alkyl, O, NH or S; X represents O, NH, or S and R⁵ represents a carboxy protecting group, comprising reacting a compound of formula 2:

HoC tf — NH

O wherein Rl, R2, X and Y are as previously described, with an activated oxalic acid agent in the presence of a base to produce a compound of formula 5

HoC

and reacting a compound of formula 5, wherein R¹, R², R⁵, X and Y are as previously described with a phosphite or phosphonite reagent to produce a compound of formula 6.

7>

O

wherein R¹ represents H or a suitable protecting group for an alcohol; R² represents a benzyl, C^ alkyl or aryl; Y represents C^_g alkyl, O, NH or S; X represents O, NH, or S and R⁵ represents a carboxy protecting group, comprising reacting a compound of formula 1:

- 8 - )R¹ o H H Ω

HoC .A^ ^" R

7> ^•NH

O

wherein R¹ is described above and R⁴ represents C^ alkyl, aryl or C_ι_₆ aralkyl; with a compound of formula 3:

T V X

wherein R², X and Y are as previously defined in the presence of WZ₄ and an amine to produce a compound of formula 2:

HoC

wherein W is a titanium, zirconium or hafnium metal and Z represents halo, sulfonate, alkoxy, aryloxy or combination thereof, and Rl, R2, X and Y are as previously described, reacting a compound of formula 2 with an activated oxalic acid agent in the presence of a base to produce a compound of formula 5 HoC

R⁵

OR¹

X

HΛ Ό Λ YR^< o

^~ N, o -.0

5 0^0- R⁵ wherein R¹ represents H or a suitable protecting group for an alcohol; R² represents a benzyl, C^g alkyl or aryl; Y represents C^g alkyl, O, NH or S; X represents O, NH, or S and R⁵ represents a carboxy protecting group, comprising reacting a compound of formula 1:

„_Λ V ^LR'

^■NH

O

- 10 - wherein R¹ is described above and R⁴ represents C₁.₁₅ alkyl, aryl or C_{1 6} ar alkyl; with a compound of formula 3:

O Ύ x ^R:

wherein W is a titanium, zirconium or hafnium metal and Z represents halo, sulfonate, alkoxy, aryloxy or combination thereof, and Rl, R2, X and Y are as previously described, and reacting a compound of formula 2 with an oxalimide forming agent in the presence of a base to produce a compound of formula 5.

Suitable amines includes trialkylamines such as triethylamine, tributylamine, trimethylamine, ethyl dimethylamine, tri- n-propylamine, di-isopropylethylamine, aniline, N_jN-di-C^_g- alkylanilines such as N,N-diethylaniline and the like.

Suitable bases include trialkylamines such as triethylamine, trimethylamine, ethyldimethylamine, tri-n-propylamine and the like, l,8-diazabicyclo[5.4.0.]undec-7-ene (DBU), pyridine, imidazole, lutidine, collidine, 4-dimethylaminomethylpyridine, inorganic carbonates and bicarbonates such as sodium carbonate, sodium bicarbonate, potassium bicarbonate, potassium carbonate, and the like and tartrates such as potassium sodium tartrate, potassium tartrate, potassium bitartrate, sodium tartrate, sodium bitartrate and the like, preferably pyridine, lutidine or collidine.

11 Suitable phosphites include P(OR^a)(OR^b)(OR^c); P(OR^a)(OR^b)(NR^cR^d); P(R^a)(R^b)(R^c); catechol phosphites or catechol dimer phosphites, wherein R^a, R^b, R^c and R^d may be the same or different and represent a straight or branched chain C^g alkyl or a phenyl, both of which may be optionally substituted with, for example, a C^ alkyl.

Preferable phosphites are trialkylphosphites such as triethyl phosphite, tributyl phosphite, triisopropyl phosphite, trimethyl phosphite and the like, most preferably triethylphosphite.

Suitable phosphonites include P(OR^e)(OR^f)(R ), wherein R^e and R^f independently represent C_{1 4} alkyl, allyl, benzyl or phenyl, optionally substituted with C^ alkyl or C^ alkoxy and R^g presents C^ alkyl, trifluoromethyl or phenyl, which is optionally substituted with C_1.3 alkyl or C_1.3 alkoxy.

Suitable activated oxylic acid agents include acid and carbodiimide moieties such as oxalyl chloride and benzyl oxalyl chloride. In particular, processes of interest are those described above wherein R¹ represents an alcohol protecting group selected from the group consisting of: H, TES, TMS, TBDMS, pNB, p- nitrobenzyloxycarbonyl, allyl and allyloxycarbonyl. Other processes that are of particular interest are those described above wherein R⁵ represents an carboxylic acid protecting group selected from the group consisting of: p-nitrobenzyl (pNB), trimethylsilyl (TMS), triethylsilyl (TES), tert-butyldimethylsilyl (TBDMS), allyl, p-methoxybenzyl, benzyl, trichloroethyl, 2- trimethylsilyl ethyl, and the like.

Still other processes that are of particular interest are those described above wherein X represents O.

Still other processes that are of particular interest are those described above wherein Y represents O or CH₂. Still other processes that are of particular interest are those described above wherein Y represents O.

Still other processes that are of particular interest are those described above wherein W represents zirconium metal.

12 - Still other processes that are of particular interest are those described above wherein W represents titanium metal.

Still other processes that are of particular interest are those described above wherein W represents hafnium metal.

Still other processes that are of particular interest are those described above wherein Z represents a halogen, most preferably chloride.

The process of the present invention is illustrated by the following generic scheme:

SCHEME A

3H

O

1.^" O OR² c ^H H oAc .^H H

H, O T o H₃C R²

-NH Metaltetrahalide ^ — NH Trialkylamine O TESCl j

2. Acid

3TES

O

DTES H .

O U

H C θ"^O ²

H

H₃C .A OR² p-nitrobenzyloxalyl chloride O n *^" o j— NH

Trialkylphosphite reagent

H,C O Ϋ

C0₂R⁵

l-Hydroxy-2-butanone is readily available and can be suitably protected by a number of synthetic methods. (3R,4R)-4-acetoxy- 3- [(R)-(tertbutylmethylsilyloxy)ethyl] -2-azetidinone and (3R,4R)-4- acetoxy-3-[(R)-(hydroxyethyl]-2-azetidinone are both readily available and undergo the addition reaction with high diastereoselectivity and in high yield.

13 - Typical conditions for the reaction involve generation of the titanium, zirconium or hafnium enolate of a suitably protected derivative of 1-hydroxybutanone such as an alkyl or aryl carbonate, preferably ethyl carbonate or isobutylcarbonate. This can be achieved by the addition of the corresponding metal tetrahalide to the derivative of 1- hydroxybutanone followed by addition of a trialkylamine. The stoichiometry of the enolate formation requires at about 0.5 to 3.0 equivalents, preferably 1 to 2.0 equivalents of metal tetrahalide. About 0.5 to about 5 equivalents, preferably about 1 to about 3 equivalents and most preferably about 1 to about 2.0 equivalents of trialkyl amine is used. The enolate generation is generally carried out at a temperature of about -80°C to about 60°C, preferably about -40°C to about 30°C.

Generally, the azetidinone is added to the enolate and the reaction temperature warmed to about 0°C - 30°C. The stoichiometry of the reaction requires about 1.0 to about 5 equivalents, preferably about 1 to about 2.0 equivalents of the enolate of the alkyl or aryl carbonate of 1- hydroxybutanone or its synthetic equivalent.

Suitable solvents for the reaction include aromatic solvents such as benzene, toluene, xylene and the like, ethereal solvents such as tetrahydrofuran (THF), diethyl ether, dioxane and the like and haloalkyl solvents such as 1,2 dichloroethane, dichloromethane, chloroform, and the like, preferably the aromatic solvents.

In a typical reaction, the azetidinone is reacted with, for example, a titanium enolate of the ethyl or isobutyl carbonate of 1- hydroxy-2-butanone, preferably the isobutyl carbonate moiety. The protecting group (e.g. TBDMS) is then preferably removed by the addition of an acid such as hydrofluoric acid (HF), HC1, or fluorosilicic acid (H₂SiF₆ ) and subsequently reprotected with another alcohol protecting group (e.g. TES derivative, typically using TESC1, benzyl ethers or allyl ethers), in the presence of a base such as imidazole or pyridine.

Reaction with p-nitrobenzyl oxalyl chloride affords the oxalimide, the precursor to the cyclization step. The cyclization step typically involves reacting the oxalimide in the presence of a phosphite or phosphonite reagent, preferably a trialkylphosphite agent. The stoichiometry of the

14 cyclization requires from about 2 to about 6 equivalents, preferably about 2.5 to about 5 equivalents of the phosphite or phosphonite. The cyclization is generally carried out at a temperature of about 25°C to about 200°C, depending on the nature of the phosphorus reagent used. When using a trialkylphosphite reagent the temperature is generally about 90°C to about 160°C.

The carbapenem produced in the cyclization is a key intermediate in the synthesis of anti-MRSA carbapenem antibiotics and can be readily coupled to a wide range of functional groups in via methods taught in USSN 08/825,786.

The final product may be characterized structurally by techniques such as NMR, IR, MS, and UV. For ease of handling, the final product, if not crystalline, may be lyophilized from water to afford an amorphous, easily handled solid. The compounds of the present invention are valuable intermediates for antibacterial agents that are active against various Gram-positive and to a lesser extent Gram-negative bacteria, and accordingly find utility in human and veterinary medicine.

Many of the compounds that can be made in accordance with the present invention are biologically active against

MRSA/MRCNS. In vitro antibacterial activity is predictive of in vivo activity when the compounds are administered to a mammal infected with a susceptible bacterial organism.

The invention is further described in connection with the following non-limiting examples.

EXAMPLE 1

O TTBBDI MS

O ^H r -OAc T H₃C O .X OiBu

NH TiCI₄ — NH Bu₃N o PhMe

15 (3R,4R)-4-acetoxy-3-[(R)-tertbutyldimethyl- 32.0g (0.11 mol) silyloxy)ethyl] -2-azetidinone isobutyl l-(2-oxobutane)carbonate 29.4g (0.156 mol) titanium tetrachloride (1M in toluene) 156 mL tributylamine 44 mL toluene 400 mL

Titanium tetrachloride solution was added to a solution of the isobutyl carbonate in toluene at -40°C. Tributylamine was added. The acetoxy azetidinone was then added and the reaction stirred at room temperature. After 3 hours the reaction was quenched with dilute hydrochloric acid. The toluene layer was washed with dilute HCl. The toluene layer was used in the subsequent step. Isolated prod, 13C NMR (CDC13) δ -5.0, -4.3, 11.7, 17.9, 18.8, 22.5, 25.8, 27.8, 44.6, 51.0, 61.7, 65.4, 69.8, 74.8, 154.8, 168.3, 205.65

EXAMPLE 2

OTBDMS

H₃C O ^OiBu ^H,c OiBu

HF o O »^ — M MeeCCNN / / PPhhMMee _^~N H

O

TBDMS azetidinone isobutyl carbonate in toluene solution from above Examplel 40g in 450 mL

HF (48% aqueous) 20 mL

Acetonitrile 400 mL

To the toluene solution from Example 1 was added acetonitrile and the HF solution. After 6 hours the reaction was quenched with aq. Rochelles salt. The toluene layer was dried and the solvent was removed. The crystalline product was swished with hexanes and filtered to yield 4-[3- ((l-oxy-2-oxobutane)isobutyl carbonate)] -2-azetidinone (23.3g) as a white solid.

16 1H NMR δ 0.95 (d, 6H), 1.25 (d, 3H), 1.3 (d, 3H), 2.0 (m, 1H), 2.9 (m, 2H), 3.85 (m, 1H), 3.9 (d, 2H), 4.1 (m, 1H), 4.75 (m, 2H), 6.3 (s, 1H)

EXAMPLE 3

O OTES ϊ O

H H

O OiBu TESCI H₃C A OiBu i-_N ^H ° H o

MeCN jr—H

O

4-[3-((l-oxy-2-oxobutane)isobutyl carbonate)]- 8.04g (0.027 mol) 2-azetidinone triethylsilyl chloride 4.7 mL (0.028 mol) imidazole 2.0g (0.029 mol) acetonitrile 60 mL

To a slurry of the azetidinone in acetonitrile was added imidazole. The reaction became homogeneous and triethylsilyl chloride was added. After 2 hours the reaction was given an aqueous work up and the organics were concentrated in vacuo to afford 11. Og of TES azetidinone isobutyl carbonate.

1H NMR 6 0.5 (q, 9H), 0.9 (t, 6H), 0.9 (d, 6H), 1.2 ( 2 doublets, 6H), 2.0 (m, 1H), 2.9 (m, 2H), 3.8 (m, 1H), 3.9 (d, 2H), 4.1 (m, 1H), 4.7 (m, 2H), 6.4 (s, 1H)

EXAMPLE 4

9, o cι

O i-NH ° Pyridine ^ ° - \ °.₀

MeCN r cr O-pNB

17 TES azetidinone isobutyl carbonate ll.Og (0.027 mol) p-NB oxalylchloride 7.15g (0.029 mol) pyridine 4 mL acetonitrile 150 L

Pyridine was added to a solution of pNB oxalylchloride in acetonitrile.

After 20 minutes the TES azetidinone isobutyl carbonate was added. The reaction was given an aqueous work up, the organics were concentrated in vacuo to afford TES oxalimide isobutyl carbonate (15.2 g) as a white solid.

13C NMR δ 4.8, 6.7, 14.0, 18.8, 22.5, 27.8, 40.9, 54.4, 61.3, 64.7, 66.7, 69.9,

74.8, 123.8, 129.0, 141.2, 148.0, 154.7, 156.0, 159.4, 164.9, 204.6.

EXAMPLE 5

OTES

_H3c l!_^λ_ι -₀A_0iBu

J — N ° P(OEt)₂ p-xylene

O^O-pNB C 0₂pNB

TES oxalimide isobutyl carbonate 15.2g (0.024 mol) triethyl phosphite 8.8 mL xylene 200 mL

Triethyl phosphite was added to a solution of TES oxalimide isobutyl carbonate in xylene. The reaction was heated to 135°C for 3 hours. The reaction was given several aqueous washes, dried and the solvent removed in vacuo to afford the desired compound (12.2g). lH NMR (399.87 MHz, CDCI3) d 8.22 (m, 2 H), 7.66 (m, 2 H), 5.57 (d,

J=14.5, 1 H), 5.46 (d, J=13.7, 1 H), 5.27 (d, J=13.7, 1 H), 4.83 (dd, J=14.5, 1.2, 1 H), 4.26 (overlapping m, 2 H), 3.95 (d, J=6.8, 2 H), 3.33 (m, 1 H), 3.28

18 (dd, J=5.6, 3.2, 1 H), 1.99 ( , 1 H), 1.26 (d, J=6.0, 3 H), 1.20 (d, J=7.2, 3 H),

0.95 (t, J=8.0, 9 H), 0.60 (m, 6 H)

13C NMR (100.55 MHz, CDCI3) d 174.8, 160.4, 155.0, 147.7, 145.5, 142.6,

128.4, 128.1, 123.7, 74.5, 65.7, 65.5, 61.6, 60.7, 55.9, 40.3, 27.8, 22.5, 18.8, 15.3, 6.7, 4.9

19

Claims

WHAT IS CLAIMED IS:

1. A process of synthesizing a compound of formula 2:

)R¹

X H H

HoC^* O Λ YR^<

O

^-NH

O

is disclosed wherein R¹ represents H or a suitable protecting group for an alcohol; R² represents a benzyl, C_j.6 alkyl or aryl; Y represents C^_g alkyl, 0, NH or S; and X represents O, NH, or S comprising reacting a compound of formula 1:

O

HoC

wherein R¹ is described above and R⁴ represents C_1.15 alkyl, aryl or C^_g ar alkyl; with a compound of formula 3:

T X

wherein R², X and Y are as previously defined in the presence of WZ₄ and an amine to produce a compound of formula 2, wherein W is a titanium, zirconium or hafnium metal and Z represents halo, sulfonate, alkoxy, aryloxy or combination thereof.

- 20

2. A process of synthesizing a compound of formula 5

X X

H H₃,CC X O^YR²

^—-N_v oo

5 ^V 0^0- R⁵

wherein R¹ represents H or a suitable protecting group for an alcohol; R² represents a benzyl, C^g alkyl or aryl; Y represents C_x.3 alkyl, O, NH or S; X represents O, NH, or S and R⁵ represents a carboxy protecting group, comprising reacting a compound of formula 2:

HoC

wherein Rl, R2, X and Y are as previously described, with an activated oxalic acid agent in the presence of a base to produce a compound of formula 5.

A process of synthesizing a compound of structural formula 6

YR^Σ

21 - is disclosed wherein R¹ represents H or a suitable protecting group for an alcohol; R² represents a benzyl, C^g alkyl or aryl; Y represents C^ alkyl, O, NH or S; X represents O, NH, or S and R⁵ represents a carboxy protecting group, comprising reacting a compound of formula 5:

HoC

4. A process of synthesizing a carbapenem compound of formula 6

wherein R¹ represents H or a suitable protecting group for an alcohol; R² represents a benzyl, C^g alkyl or aryl; Y represents C_λ_₃ alkyl, O, NH or S; X represents O, NH, or S and R5 represents a carboxy protecting group, comprising reacting a compound of formula 2:

22 HoC

wherein Rl, R2, X and Y are as previously described, with an activated oxalic acid agent in the presence of a base to produce a compound of formula 5

HoC

R⁵

A process of synthesizing a carbapenem compound of formula 6

HoC YR^<

wherein R¹ represents H or a suitable protecting group for an alcohol; R² represents a benzyl, C^g alkyl or aryl; Y represents C ₃ alkyl, O, NH or

23 - S; X represents O, NH, or S and R5 represents a carboxy protecting group, comprising reacting a compound of formula 1:

X

o // ^•NH

wherein R¹ is described above and R⁴ represents C^ alkyl, aryl or C^_g ar alkyl; with a compound of formula 3:

⁰ I

wherein W is a titanium, zirconium or hafnium metal and Z represents halo, sulfonate, alkoxy, aryloxy or combination thereof, and Rl, R2, X and Y are as previously described, reacting a compound of formula 2 with an activated oxalic acid agent in the presence of a base to produce a compound of formula 5

- 24

R⁵

A process of synthesizing a carbapenem compound of formula 5:

HoC

wherein R¹ represents H or a suitable protecting group for an alcohol; R² represents a benzyl, C^g alkyl or aryl; Y represents C^_g alkyl, O, NH or S; X represents O, NH, or S and R5 represents a carboxy protecting group, comprising reacting a compound of formula 1: o

HoC c R

25 wherein R¹ is described above and R⁴ represents C_U5 alkyl, aryl or C^_g ar alkyl; with a compound of formula 3:

O ^■γ x ^R

HoC

wherein W is a titanium, zirconium or hafnium metal and Z represents halo, sulfonate, alkoxy, aryloxy or combination thereof, and Rl, R2, X and Y are as previously described, and reacting a compound of formula 2 with a pNB oxalimide forming agent in the presence of a base to produce a compound of formula 5.

7. A process in accordance with claim 1 wherein R¹ represents a member selected from the group consisting of: H, TES, TMS, TBDMS, pNB, p-nitrobenzyloxycarbonyl, allyl and allyloxycarbonyl; R⁵ represents a carboxylic acid protecting group selected from the group consisting of: p-nitrobenzyl (PNB), benzyl, trimethylsilyl (TMS), triethylsilyl (TES), tert-butyldimethylsilyl (TBDMS), allyl, p-methoxybenzyl, trichloroethyl, and 2- trimethylsilylethyl; R² represents C^g alkyl and R⁴ represents a C_{1 5} alkyl, aryl or aralkyl.

8. A process in accordance with claim 1 wherein X represents O.

26 -

9. A process in accordance with claim 1 wherein Y represents O or CH₂.

10. A process in accordance with claim 9 wherein Y represents O.

11. A process in accordance with claim 1 wherein W represents zirconium metal.

12. A process in accordance with claim 1 wherein W represents titanium metal.

13. A process in accordance with claim 1 wherein W represents hafnium metal.

14. A process in accordance with claim 1 wherein Z represents a halogen.

15. A process in accordance with claim 14 wherein Z represents chloride.

16. A process in accordance with claim 1 wherein the amine represents triethylamine, tributylamine, trimethylamine, ethyl dimethylamine, tri-n-propylamine, di-isopropylethylamine, aniline,and N,N-dialkylanilines.

17. A process in accordance with claim 7 wherein R¹ represents a member selected from the group consisting of: H, TES, TMS, TBDMS, and pPNB; R⁵ represents p-nitrobenzyl (pNB) or benzyl; R² represents ethyl or isobutyl; and R⁴ represents acyl.

- 27

18. A process in accordance with claim 1 which is further reacted with an acid to produce in place of compound 2 a compound of formula 2a

OR"*

X

H

HoC O X YR^<

O

7 - NH

O

2a wherein R^la represents H, TES, TMS, TBDMS, or pNB, provided that R¹ and R^la are not the same.

19. A process in accordance with claim 18 wherein the acid presents hydrofluoric acid or fluorosilicic acid or HCl.

20. A process of synthesizing a compound of formula 2:

HoC

is disclosed wherein R¹ represents a member selected from the group consisting of: H, TES, TMS, TBDMS, and pNB; R² represents ethyl or isobutyl; Y represents O; and X represents O comprising reacting a compound of formula 1:

f X

^■NH

O

28 - wherein R¹ is described above and R⁴ represents C_{l 5} alkyl, aryl or C^_g ar alkyl; with a compound of formula 3:

O Ύ x ^R

wherein R², X and Y are as previously defined in the presence of WZ₄ and an amine to produce a compound of formula 2, wherein W is a titanium and Z represents chloride.

21. A process in accordance with claim 20 which is further reacted with an acid to produce in place of compound 2 a compound of formula 2 a

HoC

2a wherein R^la represents H, TES, TMS, TBDMS, and pNB, provided that R¹ and R^la are not the same and the acid presents hydrofluoric acid, hydrochloric acid or fluorosilicic acid.

22. A process in accordance with claim 20 wherein the amine represents triethylamine, tributylamine, trimethylamine, ethyldimethylamine, tri-n-propylamine, di-isopropylethylamine, aniline, and N,N-dialkylanilines.

23. A process in accordance with claim 2 wherein the base represents triethylamine, trimethylamine, ethyldimethylamine, tri-n-propylamine, l,8-diazabicyclo[5.4.0.]undec-7-ene (DBU), pyridine, imidazole, lutidine, collidine, 4-dimethylaminomethyl pyridine, sodium carbonate, sodium bicarbonate, potassium bicarbonate, potassium

29 carbonate, potassium sodium tartrate, potassium tartrate, potassium bitartrate, sodium tartrate or sodium bitartrate and the oxalic acid agent represents pNB oxalyl chloride or benzyl oxalyl chloride.

24. A process according to claim 20 wherein the base is pyridine, lutidine or collidine.

25. A process according to claim 3 wherein the phosphite represents P(OR^a)(OR^b)(OR^c); P(OR^a)(OR^b)(NR^cR^d); P(R^a)(R^b)(R^c); catechol phosphites or catechol dimer phosphites, wherein R^a, R^b, R^c and R^d may be the same or different and represent a straight or branched chain C^_g alkyl or a phenyl, both of which may be optionally substituted with, for example, a C^g alkyl.

26. A process according to claim 25 wherein the phosphites are trialkylphosphites such as triethyl phosphite, tributyl phosphite, triisopropyl phosphite, and trimethyl phosphite.

27 A process according to claim 3 wherein the phosphonites represent P(OR^e)(OR^f)(R^ε), wherein R^e and R^f independently represent C_w alkyl, allyl, benzyl or phenyl, optionally substituted with C^_g alkyl or C^ alkoxy and R^g presents Cl-4 alkyl, trifluorom ethyl or phenyl, which is optionally substituted with C^ alkyl or C_x.3 alkoxy.

28. A process of synthesizing a carbapenem compound of formula 6

- 30 wherein R¹ represents a member selected from the group consisting of: H, TES, TMS, TBDMS, and pNB; R² represents ethyl or isobutyl; Y represents O; and X represents 0; and R⁵ represents p-nitrobenzyl (PNB) or benzyl comprising reacting a compound of formula 1:

HoC

^'<V

O Y^YR'

X

wherein R², X and Y are as previously defined, in the presence of WZ₄ and an amine to produce a compound of formula 2, wherein W is a titanium and Z represents chloride to produce a compound of formula 2

HoC

reacting a compound of formula 2 with an pNB oxalyl chloride in the presence of a base such as pyridine, lutidine or collidine to produce a compound of formula 5

31 HoC

R⁵

and reacting a compound of formula 5, wherein R¹, R², R⁵, X and Y are as previously described with triethylphosphite to produce a compound of formula 6.

29. A process according to claim 28 wherein the amine represents triethylamine, tributylamine, trimethylamine, ethyldimethylamine, tri-n-propylamine, di-isopropylethylamine, aniline, or N,N-dialkylanilines.

30. A process in accordance with claim 28 which is further reacted with an acid to produce in place of compound 2 a compound of formula 2a

HoC

O

2a wherein R^la represents H, TES, TMS, TBDMS, or pNB, provided that R¹ and R^la are not the same and the acid presents hydrofluoric acid, HCl or fluorosilicic acid.

32 -