EP1043582A3 - Diffusion-controlling sensor layer - Google Patents

Diffusion-controlling sensor layer Download PDF

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Publication number
EP1043582A3
EP1043582A3 EP00105379A EP00105379A EP1043582A3 EP 1043582 A3 EP1043582 A3 EP 1043582A3 EP 00105379 A EP00105379 A EP 00105379A EP 00105379 A EP00105379 A EP 00105379A EP 1043582 A3 EP1043582 A3 EP 1043582A3
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EP
European Patent Office
Prior art keywords
substances
diffusion
sensor layer
biological
effect
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP00105379A
Other languages
German (de)
French (fr)
Other versions
EP1043582B1 (en
EP1043582A2 (en
Inventor
Wolfgang Dr. Kreiss
Günther Dr. Eberz
Claus Dr. Weisemann
Klaus Dr. Burger
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer AG
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Bayer AG
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Publication date
Application filed by Bayer AG filed Critical Bayer AG
Priority to EP02014011A priority Critical patent/EP1243926A3/en
Priority to DK00105379T priority patent/DK1043582T3/en
Publication of EP1043582A2 publication Critical patent/EP1043582A2/en
Publication of EP1043582A3 publication Critical patent/EP1043582A3/en
Application granted granted Critical
Publication of EP1043582B1 publication Critical patent/EP1043582B1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/18—Testing for antimicrobial activity of a material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Toxicology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Fluid Adsorption Or Reactions (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Glass Compositions (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)

Abstract

Die Erfindung betrifft eine diffusionskontrollierende Sensorschicht zur Erfassung der biologischen Wirkung von Substanzen, bestehend aus einer diffusionskontrollierenden Matrix und darin suspendierten Sensoren. Die Erfindung besteht weiterhin in einem Verfahren und einer Vorrichtung zur ortsabhängigen Erfassung der biologischen Wirkung von Substanzen mittels der erfindungsgemäßen Sensorschicht (

Figure 80000001
Abbildung" der örtlichen Verteilung von biologischer Aktivität). Eine weitere Ausführungsform des erfindungsgemäßen Verfahrens betrifft die Kopplung von Untersuchungen der biologischen Wirksamkeit von Substanzen mit chromatographischen und elektrophoretischen Trenntechniken, so dass biologisch aktive Einzelsubstanzen direkt in Gemischen identifiziert werden können. Eine zusätzliche Ausführungsform des erfindungsgemäßen Verfahrens beinhaltet die unmittelbare Kombination der Wirkungsdetektion über Sensorschichten mit chromatographischen und elektrophoretischen Trenntechniken, die ihrerseits mit spektroskopischen Techniken gekoppelt sind. Diese enge Kopplung aus Stofftrennung, Wirkungsdetektion und Spektroskopie liefert direkt Strukturergebnisse zu den biologisch aktiven Substanzen eines Stoffgemischs.
Figure 00000001
The invention relates to a diffusion-controlling sensor layer for detecting the biological effect of substances, consisting of a diffusion-controlling matrix and sensors suspended therein. The invention further consists in a method and a device for the location-dependent detection of the biological effect of substances by means of the sensor layer according to the invention (
Figure 80000001
Figure "of the local distribution of biological activity). A further embodiment of the method according to the invention relates to the coupling of studies of the biological effectiveness of substances with chromatographic and electrophoretic separation techniques, so that biologically active individual substances can be identified directly in mixtures. An additional embodiment of the method according to the invention includes the direct combination of effect detection via sensor layers with chromatographic and electrophoretic separation techniques, which in turn are coupled with spectroscopic techniques.This close coupling of material separation, effect detection and spectroscopy provides structural results directly for the biologically active substances of a substance mixture.
Figure 00000001
EP00105379A 1999-04-03 2000-03-21 Diffusion-controlling sensor layer Expired - Lifetime EP1043582B1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP02014011A EP1243926A3 (en) 1999-04-03 2000-03-21 Diffusion-controlling sensor layer
DK00105379T DK1043582T3 (en) 1999-04-03 2000-03-21 Diffusion controlling sensor layer

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19915310 1999-04-03
DE19915310A DE19915310A1 (en) 1999-04-03 1999-04-03 Diffusion-controlling sensor layer

Related Child Applications (1)

Application Number Title Priority Date Filing Date
EP02014011A Division EP1243926A3 (en) 1999-04-03 2000-03-21 Diffusion-controlling sensor layer

Publications (3)

Publication Number Publication Date
EP1043582A2 EP1043582A2 (en) 2000-10-11
EP1043582A3 true EP1043582A3 (en) 2001-05-23
EP1043582B1 EP1043582B1 (en) 2004-08-18

Family

ID=7903543

Family Applications (2)

Application Number Title Priority Date Filing Date
EP00105379A Expired - Lifetime EP1043582B1 (en) 1999-04-03 2000-03-21 Diffusion-controlling sensor layer
EP02014011A Withdrawn EP1243926A3 (en) 1999-04-03 2000-03-21 Diffusion-controlling sensor layer

Family Applications After (1)

Application Number Title Priority Date Filing Date
EP02014011A Withdrawn EP1243926A3 (en) 1999-04-03 2000-03-21 Diffusion-controlling sensor layer

Country Status (10)

Country Link
US (1) US20070292912A1 (en)
EP (2) EP1043582B1 (en)
JP (1) JP2000321208A (en)
AT (1) ATE274183T1 (en)
CA (1) CA2303529A1 (en)
DE (2) DE19915310A1 (en)
DK (1) DK1043582T3 (en)
ES (1) ES2226626T3 (en)
IL (1) IL135394A0 (en)
PT (1) PT1043582E (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4552363B2 (en) * 2001-06-15 2010-09-29 株式会社島津製作所 Liquid chromatograph mass spectrometer
DE10133273A1 (en) * 2001-07-09 2003-01-30 Bayer Cropscience Ag Device and method for the detection of photosynthesis inhibition
FR2856792B1 (en) * 2003-06-27 2006-09-15 Commissariat Energie Atomique METHOD AND DEVICE FOR ASSAYING A BIOLOGICAL OR CHEMICAL SAMPLE

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4162208A (en) * 1975-02-03 1979-07-24 Aladjem Frederick J Quantitative protein analysis by immunodiffusion
US5578460A (en) * 1993-09-23 1996-11-26 E. I. Du Pont De Nemours And Company Electrophoretic method for the isolation and separation of microorganisms and cell populations
US5766963A (en) * 1996-01-26 1998-06-16 Pharmacopeia, Inc. Combination hydroxypropylamine library

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2948904C2 (en) * 1979-12-05 1986-06-19 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V., 3400 Göttingen Device for the optical measurement of concentrations of substances
JPH076984B2 (en) * 1985-05-31 1995-01-30 株式会社日立製作所 Multiple item analysis method
GB8621261D0 (en) * 1986-09-03 1986-10-08 Barnard G J R Enhanced luminescent assay
US5156810A (en) * 1989-06-15 1992-10-20 Biocircuits Corporation Biosensors employing electrical, optical and mechanical signals
US5330718A (en) * 1989-08-16 1994-07-19 Puritan-Bennett Corporation Sensor element and method for making the same
GB2244135B (en) * 1990-05-04 1994-07-13 Gen Electric Co Plc Sensor devices
DE4221036A1 (en) * 1992-06-26 1994-01-05 Siemens Ag Image processing arrangement
AT399402B (en) * 1992-07-09 1995-05-26 Avl Verbrennungskraft Messtech SENSOR MEMBRANE OF AN OPTICAL SENSOR FOR DETERMINING A PHYSICAL OR CHEMICAL PARAMETER OF A SAMPLE
DE4230264A1 (en) * 1992-09-10 1994-03-17 Bayer Ag Analytical method for the investigation of mixtures for toxic components
US5565324A (en) * 1992-10-01 1996-10-15 The Trustees Of Columbia University In The City Of New York Complex combinatorial chemical libraries encoded with tags
AT401526B (en) * 1993-02-10 1996-09-25 Scheirer Winfried REAGENT SOLUTION TO STABILIZE LUMINESCENCE IN LUCIFERASE MEASUREMENT
DE4304728C2 (en) * 1993-02-13 1997-04-10 Igor Dr Popov Method and test kit for the determination of ascorbic acid in biological samples
DE19631395A1 (en) * 1996-08-02 1998-02-05 Basf Ag Drug screening process
JP2000505902A (en) * 1996-12-05 2000-05-16 イデゴ・アーペーエス Sensor stack and multi-compartment fluid delivery device for detecting target molecules in biological fluids by immunoassay
ATE269975T1 (en) * 1997-04-07 2004-07-15 Bioimage As METHOD FOR SCREENING SUBSTANCES THAT HAVE AN EFFECT ON INTRACELLULAR TRANSLOCATION
US6200762B1 (en) * 1997-08-01 2001-03-13 Aurora Biosciences Corporation Photon reducing agents and compositions for fluorescence assays
DE19734800A1 (en) * 1997-08-12 1999-02-18 Heide Prof Dr Schnabl Detection of environmental toxins in soil, composts and substrates
US6117643A (en) * 1997-11-25 2000-09-12 Ut Battelle, Llc Bioluminescent bioreporter integrated circuit
US5942409A (en) * 1998-07-31 1999-08-24 Byk Gulden Lomberg Chemische Fabrik Gmbh Process for identification of substances modulating ureI dependent mechanisms of Helicobacter pylori metabolism
DE29815139U1 (en) * 1998-08-14 1999-01-14 I.U.T. - Privates Institut für Umwelttoxikologie GmbH, 17489 Greifswald Device for detecting the toxic and mutagenic effects of chemicals and mixtures of substances

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4162208A (en) * 1975-02-03 1979-07-24 Aladjem Frederick J Quantitative protein analysis by immunodiffusion
US5578460A (en) * 1993-09-23 1996-11-26 E. I. Du Pont De Nemours And Company Electrophoretic method for the isolation and separation of microorganisms and cell populations
US5766963A (en) * 1996-01-26 1998-06-16 Pharmacopeia, Inc. Combination hydroxypropylamine library

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
P.D. SHAW ET AL.: "Detecting and characterizing N-acyl-homoserine lactone signal molecules by thin-layer chromatography", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 94, no. 12, 1997, NATIONAL ACADEMY OF SCIENCE, WASHINGTON, DC, US, pages 6036 - 6041, XP000990381, ISSN: 0027-8424 *

Also Published As

Publication number Publication date
DK1043582T3 (en) 2004-12-06
DE50007429D1 (en) 2004-09-23
EP1043582B1 (en) 2004-08-18
EP1243926A2 (en) 2002-09-25
IL135394A0 (en) 2001-05-20
US20070292912A1 (en) 2007-12-20
CA2303529A1 (en) 2000-10-03
DE19915310A1 (en) 2000-10-05
PT1043582E (en) 2004-12-31
EP1043582A2 (en) 2000-10-11
EP1243926A3 (en) 2003-09-03
ES2226626T3 (en) 2005-04-01
JP2000321208A (en) 2000-11-24
ATE274183T1 (en) 2004-09-15

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