EP1034308A4 - Method of generating conditionally expressed mutant cells using expressible antisense sequences - Google Patents

Method of generating conditionally expressed mutant cells using expressible antisense sequences

Info

Publication number
EP1034308A4
EP1034308A4 EP98962914A EP98962914A EP1034308A4 EP 1034308 A4 EP1034308 A4 EP 1034308A4 EP 98962914 A EP98962914 A EP 98962914A EP 98962914 A EP98962914 A EP 98962914A EP 1034308 A4 EP1034308 A4 EP 1034308A4
Authority
EP
European Patent Office
Prior art keywords
antisense sequences
mutant cells
expressed mutant
conditionally expressed
generating conditionally
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP98962914A
Other languages
German (de)
French (fr)
Other versions
EP1034308A1 (en
Inventor
Andrea Marra
Martin Rosenberg
Yinduo Ji
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SmithKline Beecham Corp
Original Assignee
SmithKline Beecham Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SmithKline Beecham Corp filed Critical SmithKline Beecham Corp
Publication of EP1034308A1 publication Critical patent/EP1034308A1/en
Publication of EP1034308A4 publication Critical patent/EP1034308A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Environmental Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Immunology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
EP98962914A 1997-12-04 1998-12-04 Method of generating conditionally expressed mutant cells using expressible antisense sequences Withdrawn EP1034308A4 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US6744697P 1997-12-04 1997-12-04
US67446P 1997-12-04
US8253498P 1998-04-20 1998-04-20
US82534P 1998-04-20
US10516198P 1998-10-21 1998-10-21
US105161P 1998-10-21
PCT/US1998/025808 WO1999028508A1 (en) 1997-12-04 1998-12-04 Method of generating conditionally expressed mutant cells using expressible antisense sequences

Publications (2)

Publication Number Publication Date
EP1034308A1 EP1034308A1 (en) 2000-09-13
EP1034308A4 true EP1034308A4 (en) 2002-04-03

Family

ID=27371157

Family Applications (1)

Application Number Title Priority Date Filing Date
EP98962914A Withdrawn EP1034308A4 (en) 1997-12-04 1998-12-04 Method of generating conditionally expressed mutant cells using expressible antisense sequences

Country Status (3)

Country Link
EP (1) EP1034308A4 (en)
JP (1) JP2002503447A (en)
WO (1) WO1999028508A1 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6139817A (en) * 1996-10-15 2000-10-31 Smithkline Beecham Corporation Method for determining gene essentiality in a pathogen
US6228579B1 (en) 1997-11-14 2001-05-08 San Diego State University Foundation Method for identifying microbial proliferation genes
US6720139B1 (en) 1999-01-27 2004-04-13 Elitra Pharmaceuticals, Inc. Genes identified as required for proliferation in Escherichia coli
AU2002241706A1 (en) * 2000-12-27 2002-07-08 Elitra Pharmaceuticals, Inc. Bacterial promoters and methods of use
WO2002083940A2 (en) * 2001-04-06 2002-10-24 Creatogen Aktiengesellschaft Screening method for anti-microbial drug targets by genome-saturing mutagenesis (gsm)
US7238482B2 (en) 2003-05-07 2007-07-03 E. I. Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
AU2004290052B2 (en) 2003-11-12 2008-12-04 Corteva Agriscience Llc Delta-15 desaturases suitable for altering levels of polyunsaturated fatty acids in oleaginous plants and yeast
US7550286B2 (en) 2004-11-04 2009-06-23 E. I. Du Pont De Nemours And Company Docosahexaenoic acid producing strains of Yarrowia lipolytica
US9175318B2 (en) 2008-12-18 2015-11-03 E. I. Dupont De Nemours And Company Reducing byproduction of malonates by yeast in a fermentation process
US20120247066A1 (en) 2011-04-01 2012-10-04 Ice House America, Llc Ice bagging apparatus and methods
CN105018528B (en) * 2015-08-20 2018-04-03 重庆大学 Heat shock protein gene promoter and the multi-gene expression and silencing system of the control of tetracycline gene promoter

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998021366A1 (en) * 1996-11-13 1998-05-22 Qbi Enterprises, Ltd. Gene identification method
WO1999027135A2 (en) * 1997-11-21 1999-06-03 Yale University Method for identifying and inhibiting functional nucleic acid molecules in cells

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5639595A (en) * 1990-05-01 1997-06-17 Isis Phamaceuticals, Inc. Identification of novel drugs and reagents

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998021366A1 (en) * 1996-11-13 1998-05-22 Qbi Enterprises, Ltd. Gene identification method
WO1999027135A2 (en) * 1997-11-21 1999-06-03 Yale University Method for identifying and inhibiting functional nucleic acid molecules in cells

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DG. PESTOV AND LF LAU: "Genetic selection of growth-inhibitory sequences in mammalian cells", PROC. NATL. ACAD. SCI USA, vol. 91, no. 26, 20 December 1994 (1994-12-20), pages 12549 - 12553, XP002188815 *
DS. KERNODLE ET AL.,: "Expression of an antisense hla fragment in Staphylococcus aureus reduces alpha-toxin production in vitro and attenuates lethal activity in a murine model", INFECTION AND IMMUNITY, vol. 65, no. 1, January 1997 (1997-01-01), pages 179 - 184, XP002188816 *
See also references of WO9928508A1 *
T. PARISH AND NG STOKER: "Development and use of a conditional antisense mutagenesis system in mycobacteria", FEMS MICROBIOL LETT, vol. 154, no. 1, 1 September 1997 (1997-09-01), pages 151 - 157, XP001055684 *
TP. SPANN ET AL.,: "Mutagenesis and gene identification in Dictyostelium by shotgun antisense", PROC. NATL. ACAD. SCI. USA, vol. 93, no. 10, May 1996 (1996-05-01), pages 5003 - 5007, XP002188814 *
Y. JI ET AL.,: "Identification of critical Staphylococcal genes using conditional phenotypes generated by antisense RNA", SCIENCE, vol. 293, no. 5538, 21 September 2001 (2001-09-21), pages 2266 - 2269, XP002188817 *

Also Published As

Publication number Publication date
EP1034308A1 (en) 2000-09-13
WO1999028508A1 (en) 1999-06-10
JP2002503447A (en) 2002-02-05

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