EP0924303A3 - Method for detecting nucleic acids - Google Patents

Method for detecting nucleic acids Download PDF

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Publication number
EP0924303A3
EP0924303A3 EP98120922A EP98120922A EP0924303A3 EP 0924303 A3 EP0924303 A3 EP 0924303A3 EP 98120922 A EP98120922 A EP 98120922A EP 98120922 A EP98120922 A EP 98120922A EP 0924303 A3 EP0924303 A3 EP 0924303A3
Authority
EP
European Patent Office
Prior art keywords
nucleic acid
nucleic acids
detecting nucleic
conjugate
binds
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP98120922A
Other languages
German (de)
French (fr)
Other versions
EP0924303A2 (en
Inventor
Erasmus Dr. Huber
Frank Dr. Bergmann
Christoph Dr. Seidel
Dieter Dr. Heindl
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Roche Diagnostics GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics GmbH filed Critical Roche Diagnostics GmbH
Publication of EP0924303A2 publication Critical patent/EP0924303A2/en
Publication of EP0924303A3 publication Critical patent/EP0924303A3/en
Withdrawn legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Ein Verfahren zum Nachweis einer Nukleinsäure mit Hilfe eines Konjugats aus einem nukleinsäurebindenden Rest und einer chemischen Gruppe, welche selbst nicht an die nachzuweisende Nukleinsäure bindet, aber bei Bindung des Konjugats an die Nukleinsäure eine neue, nachweisbare Eigenschaft erhält, ermöglicht eine homogene Versuchsführung.A method for the detection of a nucleic acid using a conjugate from a nucleic acid binding residue and a chemical group, which itself does not belong to the The nucleic acid to be detected binds, but when the conjugate binds to the nucleic acid receives a new, demonstrable property, enables a homogeneous test procedure.

EP98120922A 1997-11-07 1998-11-04 Method for detecting nucleic acids Withdrawn EP0924303A3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19749163 1997-11-07
DE19749163A DE19749163A1 (en) 1997-11-07 1997-11-07 Detection of nucleic acids with a conjugated probe

Publications (2)

Publication Number Publication Date
EP0924303A2 EP0924303A2 (en) 1999-06-23
EP0924303A3 true EP0924303A3 (en) 2002-09-18

Family

ID=7847876

Family Applications (1)

Application Number Title Priority Date Filing Date
EP98120922A Withdrawn EP0924303A3 (en) 1997-11-07 1998-11-04 Method for detecting nucleic acids

Country Status (3)

Country Link
EP (1) EP0924303A3 (en)
JP (1) JPH11221098A (en)
DE (1) DE19749163A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10125258A1 (en) 2001-05-23 2003-01-09 November Ag Molekulare Medizin Method for determining the binding behavior of ligands that specifically bind to target molecules

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0530998A1 (en) * 1991-08-15 1993-03-10 Microgenics Corporation Detection of complementary nucleotide sequences

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0530998A1 (en) * 1991-08-15 1993-03-10 Microgenics Corporation Detection of complementary nucleotide sequences

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
COREY D R: "Peptide nucleic acids: expanding the scope of nucleic acid recognition", TRENDS IN BIOTECHNOLOGY, ELSEVIER PUBLICATIONS, CAMBRIDGE, GB, vol. 15, no. 6, 1 June 1997 (1997-06-01), pages 224 - 229, XP004064511, ISSN: 0167-7799 *
TYAGI S ET AL: "MOLECULAR BEACONS: PROBES THAT FLUORESCE UPON HYBRIDIZATION", NATURE BIOTECHNOLOGY, NATURE PUBLISHING, US, vol. 14, 1 March 1996 (1996-03-01), pages 303 - 308, XP000196024, ISSN: 1087-0156 *

Also Published As

Publication number Publication date
JPH11221098A (en) 1999-08-17
EP0924303A2 (en) 1999-06-23
DE19749163A1 (en) 1999-05-12

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