EP0832190A1 - Oligonucleotides essentiels de la telomerase de vertebre - Google Patents

Oligonucleotides essentiels de la telomerase de vertebre

Info

Publication number
EP0832190A1
EP0832190A1 EP96918335A EP96918335A EP0832190A1 EP 0832190 A1 EP0832190 A1 EP 0832190A1 EP 96918335 A EP96918335 A EP 96918335A EP 96918335 A EP96918335 A EP 96918335A EP 0832190 A1 EP0832190 A1 EP 0832190A1
Authority
EP
European Patent Office
Prior art keywords
telomerase
rna
dna
htr
activity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP96918335A
Other languages
German (de)
English (en)
Inventor
Carol Greider
Chantal Autexier
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cold Spring Harbor Laboratory
Original Assignee
Cold Spring Harbor Laboratory
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cold Spring Harbor Laboratory filed Critical Cold Spring Harbor Laboratory
Publication of EP0832190A1 publication Critical patent/EP0832190A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Definitions

  • telomere protein components can be produced by combining telomerase protein components with oligonucleotides prepared by recombinant methods, oligonucleotides which are isolated from sources in which they occur in nature or oligonucleotides which are synthetically produced. Similar types of truncated telomerases can be constructed by combining truncated oligonucleotides from other vertebrate telomerase RNA components with telomerase protein.
  • Figure 2 shows the activity of telomerase reconstituted with telomerase RNA mutations and assayed in the absence or presence of dATP.
  • PCR conditions were as described for phTR+l except 10 ng of the template fragment was used and the cycling conditions were the following: 5 cycles at 94 * C 40 sec, 54 * C 20 sec, 72 * C 60 sec, followed by 25 cycles 94"C 40 sec, 60 * C 20 sec, 72 * C 60 sec.
  • Primers hTR170 and TRC31 were used in PCR for constructing phTR170. The sequences of hTR170 and TRC31 are
  • RNAs made from pUC119 based plasmids were the following: hTRl-159 (Xbal 159) , hTRl-169 (B vl 169), hTRl-l ⁇ 2 (PvuII 182) , hTRl-203 (Smal 203), hTRl-273 (BspEl 273) and hTRl-445 (Fspl 445) .
  • the Fspl site at position 445 was created by site-directed mutagenesis.
  • the TGCAGT spanning nucleotides 443 to 448 was altered to TGCGCA which is cut by Fspl.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Wood Science & Technology (AREA)
  • Public Health (AREA)
  • Genetics & Genomics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

L'invention porte sur des séquences d'ADN codant pour des oligonucléotides d'un ARN tronqué correspondant à la partie ARN de la télomérase de vertébré qui est essentielle pour son fonctionnement, ainsi que l'utilisation de ces oligonucléotides. L'invention porte également sur la télomérase de vertébrés produite en combinant l'oligonucléotide d'ARN ou la partie ARN isolée avec la protéine de cette télomérase de vertébré.
EP96918335A 1995-06-07 1996-06-06 Oligonucleotides essentiels de la telomerase de vertebre Withdrawn EP0832190A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US47835295A 1995-06-07 1995-06-07
US478352 1995-06-07
PCT/US1996/009517 WO1996040868A1 (fr) 1995-06-07 1996-06-06 Oligonucleotides essentiels de la telomerase de vertebre

Publications (1)

Publication Number Publication Date
EP0832190A1 true EP0832190A1 (fr) 1998-04-01

Family

ID=23899594

Family Applications (1)

Application Number Title Priority Date Filing Date
EP96918335A Withdrawn EP0832190A1 (fr) 1995-06-07 1996-06-06 Oligonucleotides essentiels de la telomerase de vertebre

Country Status (5)

Country Link
EP (1) EP0832190A1 (fr)
JP (1) JPH11507818A (fr)
AU (1) AU701903B2 (fr)
CA (1) CA2221602A1 (fr)
WO (1) WO1996040868A1 (fr)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6517834B1 (en) 1995-08-04 2003-02-11 Geron Corporation Purified telomerase
US5968506A (en) * 1995-08-04 1999-10-19 Geron Corporation Purified telomerase
US6545133B1 (en) 1995-08-04 2003-04-08 Geron Corporation Methods for purifying telomerase
US6610839B1 (en) 1997-08-14 2003-08-26 Geron Corporation Promoter for telomerase reverse transcriptase
EP1783139B8 (fr) 1996-10-01 2009-09-23 Geron Corporation Sous-unité catalytique de télomérase humaine
US7585622B1 (en) 1996-10-01 2009-09-08 Geron Corporation Increasing the proliferative capacity of cells using telomerase reverse transcriptase
US6093809A (en) 1996-10-01 2000-07-25 University Technology Corporation Telomerase
US6808880B2 (en) 1996-10-01 2004-10-26 Geron Corporation Method for detecting polynucleotides encoding telomerase
US6475789B1 (en) 1996-10-01 2002-11-05 University Technology Corporation Human telomerase catalytic subunit: diagnostic and therapeutic methods
US6261836B1 (en) 1996-10-01 2001-07-17 Geron Corporation Telomerase
CA2274586A1 (fr) * 1996-12-20 1998-07-02 Geron Corporation Procedes de detection et d'inhibition de la telomerase dans l'arn
EP0981627B1 (fr) * 1997-04-04 2003-09-17 Geron Corporation Telomerase purifiee
US7622549B2 (en) 1997-04-18 2009-11-24 Geron Corporation Human telomerase reverse transcriptase polypeptides
US7413864B2 (en) 1997-04-18 2008-08-19 Geron Corporation Treating cancer using a telomerase vaccine
AU745420B2 (en) * 1997-06-20 2002-03-21 Bayer Aktiengesellschaft Human catalytic telomerase sub-unit and its diagnostic and therapeutic use
US7378244B2 (en) 1997-10-01 2008-05-27 Geron Corporation Telomerase promoters sequences for screening telomerase modulators
JP2002500040A (ja) * 1998-01-12 2002-01-08 コールド スプリング ハーバー ラボラトリー 細胞の寿命の延長、方法および試薬
DE19804372A1 (de) * 1998-02-04 1999-08-05 Michael W Dr Dr Dahm Verfahren zur quantitativen Bestimmung von Tumorzellen in einer Körperflüssigkeit und dazu geeignete Testkits

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5489508A (en) * 1992-05-13 1996-02-06 University Of Texas System Board Of Regents Therapy and diagnosis of conditions related to telomere length and/or telomerase activity
WO1995013382A1 (fr) * 1993-11-12 1995-05-18 Geron Corporation Diagnostic et traitement de desordres lies a la longueur de telomeres et/ou l'activite de la telomerase
EP0666313A3 (fr) * 1994-01-27 1996-03-06 Univ Iowa State Res Found Inc Ciblage de la télomérase dans la thérapie génique du cancer.

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO9640868A1 *

Also Published As

Publication number Publication date
JPH11507818A (ja) 1999-07-13
WO1996040868A1 (fr) 1996-12-19
AU6102296A (en) 1996-12-30
CA2221602A1 (fr) 1996-12-19
AU701903B2 (en) 1999-02-11

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