EP0804415A1 - Novel farnesyl transferase inhibitors, preparation thereof and pharmaceutical compositions containing same - Google Patents

Novel farnesyl transferase inhibitors, preparation thereof and pharmaceutical compositions containing same

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Publication number
EP0804415A1
EP0804415A1 EP96901397A EP96901397A EP0804415A1 EP 0804415 A1 EP0804415 A1 EP 0804415A1 EP 96901397 A EP96901397 A EP 96901397A EP 96901397 A EP96901397 A EP 96901397A EP 0804415 A1 EP0804415 A1 EP 0804415A1
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EP
European Patent Office
Prior art keywords
radical
hydrogen atom
carbon atoms
alkyl
optionally substituted
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
EP96901397A
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German (de)
French (fr)
Inventor
Bernard Baudoin
Christopher Burns
Alain Commercon
Alain Lebrun
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Aventis Pharma SA
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Rhone Poulenc Rorer SA
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Publication of EP0804415A1 publication Critical patent/EP0804415A1/en
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C323/00—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups
    • C07C323/23—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and nitrogen atoms, not being part of nitro or nitroso groups, bound to the same carbon skeleton
    • C07C323/24—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and nitrogen atoms, not being part of nitro or nitroso groups, bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton
    • C07C323/25—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and nitrogen atoms, not being part of nitro or nitroso groups, bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton the carbon skeleton being acyclic and saturated
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
    • A61K31/19—Carboxylic acids, e.g. valproic acid
    • A61K31/195—Carboxylic acids, e.g. valproic acid having an amino group
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/21—Esters, e.g. nitroglycerine, selenocyanates
    • A61K31/215—Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids
    • A61K31/22—Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids of acyclic acids, e.g. pravastatin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C235/00—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms
    • C07C235/02—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms having carbon atoms of carboxamide groups bound to acyclic carbon atoms and singly-bound oxygen atoms bound to the same carbon skeleton
    • C07C235/04—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms having carbon atoms of carboxamide groups bound to acyclic carbon atoms and singly-bound oxygen atoms bound to the same carbon skeleton the carbon skeleton being acyclic and saturated
    • C07C235/12—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms having carbon atoms of carboxamide groups bound to acyclic carbon atoms and singly-bound oxygen atoms bound to the same carbon skeleton the carbon skeleton being acyclic and saturated having the nitrogen atom of at least one of the carboxamide groups bound to an acyclic carbon atom of a hydrocarbon radical substituted by carboxyl groups
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C323/00—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups
    • C07C323/50—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and carboxyl groups bound to the same carbon skeleton
    • C07C323/51—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and carboxyl groups bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton
    • C07C323/57—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and carboxyl groups bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton the carbon skeleton being further substituted by nitrogen atoms, not being part of nitro or nitroso groups
    • C07C323/58—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and carboxyl groups bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton the carbon skeleton being further substituted by nitrogen atoms, not being part of nitro or nitroso groups with amino groups bound to the carbon skeleton
    • C07C323/59—Thiols, sulfides, hydropolysulfides or polysulfides substituted by halogen, oxygen or nitrogen atoms, or by sulfur atoms not being part of thio groups containing thio groups and carboxyl groups bound to the same carbon skeleton having the sulfur atoms of the thio groups bound to acyclic carbon atoms of the carbon skeleton the carbon skeleton being further substituted by nitrogen atoms, not being part of nitro or nitroso groups with amino groups bound to the carbon skeleton with acylated amino groups bound to the carbon skeleton

Definitions

  • the present invention relates to new farnesyl transferase inhibitors of general formula:
  • the C-terminal sequence of the Ras gene contains the motif "CAAX” or "Cys-Aaa ⁇ -Aaa2-Xaa M in which Aaa represents an aliphatic amino acid and Xaa represents any amino acid. It is known that tetrapeptides with a CAAX sequence can inhibit famesylation of the Ras protein.
  • X represents an oxygen or sulfur atom
  • R2 represents an alkyl, alkenyl or straight or branched alkynyl radical containing 1 to 6 carbon atoms optionally substituted by a hydroxy radical, alkoxy containing 1 to 4 carbon atoms, mercapto, alkylthio containing 1 to 4 carbon atoms, alkylsulfinyl containing 1 to 4 carbon atoms or alkylsulfonyl containing 1 to 4 carbon atoms, it being understood that, when R2 represents an alkyl radical substituted by a hydroxy radical, R2 can form with the carboxy radical in ⁇ a lactone, R'2 represents an atom of hydrogen or a straight or branched alkyl radical containing 1 to 6 carbon atoms, and
  • R represents a hydrogen atom or an alkyl radical containing 1 to 6 carbon atoms optionally substituted by an alkoxy radical containing 1 to 4 carbon atoms, alkylthio containing 1 to 4 carbon atoms, alkylsulfinyl containing 1 to 4 carbon atoms, alkylsulfonyl containing 1 to 4 carbon atoms, phenyl, phenoxy, phenylthio, phenylsulf nyl, phenylsulfonyl, alkylamino containing 1 to 4 carbon atoms, dialkoylamino each alkyl part of which contains 1 to 4 carbon atoms, or a phenyl radical optionally substituted by a or several atoms or radicals chosen from halogen atoms and alkyl, alkyloxy, alkylthio or alkanoyl radicals.
  • Rj represents a radical of formula YS-Aj- in which Y represents a hydrogen atom or a lysine residue or a fatty acid residue containing up to 20 carbon atoms and A represents an ethylene or propylene radical optionally substituted by an amino radical,
  • R'i represents a hydrogen atom or a methyl radical
  • X represents an oxygen atom
  • R2 represents an alkyl radical containing 1 to 4 carbon atoms optionally substituted by a hydroxy, methoxy, mercapto, methylthio, memylsulfinyl or methylsulfonyl radical
  • R'2 represents a hydrogen atom or a methyl radical
  • R represents a hydrogen atom or an alkyl radical containing 1 to 4 carbon atoms, optionally substituted by an alkoxy radical, or a phenyl radical. More particularly still,
  • Rj represents a radical of formula Y-S-Aj- in which Y represents a hydrogen atom and Aj represents an ethylene or propylene radical optionally substituted by an amino radical,
  • R'i represents a hydrogen atom
  • X represents an oxygen atom
  • R2 represents a methyl, ethyl, propyl or butyl radical optionally substituted by a hydroxy, methoxy, mercapto or methylthio radical
  • R'2 represents a hydrogen atom
  • R represents a hydrogen atom or an alkyl radical containing 1 to 4 carbon atoms.
  • Rj represents a 2-mercapto-ethyl or 1-amino-2-mercapto-ethyl radical
  • R'j represents a hydrogen atom
  • X represents an oxygen atom
  • R2 represents an n.butyl or 2-methylthio-ethyl radical and R'2 represents a hydrogen
  • R represents a hydrogen atom or a methyl radical.
  • the present invention also relates to the stereoisomeric forms of the products of general formula (I).
  • R ⁇ C (X ⁇ ) (Y ⁇ ) (NR' ⁇ ) and R'2CH (NR'2) CO-OH preferably have the configuration of natural amino acids.
  • the present invention also relates to the mineral or organic salts of the products of general formula (I).
  • the new products according to the invention can be prepared by applying known methods derived from the methods used more particularly in peptide chemistry for the assembly of chains.
  • R2 and R'2 are defined as above and R 'represents an alkyl radical containing 1 to 4 carbon atoms, optionally substituted by a phenyl radical, preferably a tert-butyl radical, operating in the presence of an agent condensation, such as hydroxybenzotriazole and dicyclohexylcarbod ⁇ mide, and of a base, such as triethylamine, in an organic solvent, such as dimethylformamide, to give a product of general formula:
  • Ri-CHO (VII) in which Rj is defined as above, it being understood that the amino and mercapto functions carried by Rj are optionally protected by appropriate protective groups such as a trityl radical for the mercapto function or a tert-butoxycarbonyl radical for the amino function, on a product of general formula (V) in the presence of a reducing agent such as sodium cyanoborohydride, sodium borohydride, sodium triacetoxyborohydride or hydrogen in the presence of a catalyst.
  • a reducing agent such as sodium cyanoborohydride, sodium borohydride, sodium triacetoxyborohydride or hydrogen in the presence of a catalyst.
  • the reaction is carried out in an organic solvent such as an alcohol such as methanol optionally in combination with a other organic solvent such as an ether such as tetrahydrofuran. It is particularly advantageous to operate in an anhydrous medium.
  • the protective groups are replaced by hydrogen atoms by application of the usual techniques.
  • the Boc or trityl or tert-butyl protecting groups can be replaced by hydrogen atoms by means of trifluoroacetic acid in the presence of ethanedithiol.
  • the products of general formula (I) in which X represents a sulfur atom can be obtained from a product of general formula (III) in which X represents an oxygen atom by thionation then by performing the steps of reduction, condensation or reductive amination as appropriate and deprotection described above for the preparation of a product of general formula (I) in which X represents an oxygen atom.
  • the products of general formula (I) in which R represents a hydrogen atom can also be obtained by saponification of a product of general formula (VI) followed by the replacement of the protective groups carried by R ⁇ under the conditions described above.
  • 5-nitro-1,2,3,4-tetrahydro-naphthyl-l-carboxylic acid which can be obtained as a mixture with 7-nitro-1,2,3,4-tetrahydro-naphthyl-l acid -carboxylic, can be prepared according to the process described by T. NAKAYAMA et al., Chem. Pharm. Bull., 32, 3968 (1984).
  • the products of general formula (I) can be purified according to the usual methods such as chromatography.
  • the following examples illustrate the preparation of the products according to the invention.
  • the organic solution is washed twice with 50 cm3 of an aqueous solution of sodium hydrogencarbonate at 10% (w / v) then once with 50 cm3 of an aqueous solution of citric acid at 10%, once distilled water, and finally with a saturated aqueous solution of sodium chloride.
  • the organic phase is dried over magnesium sulfate, filtered and then concentrated to dryness under reduced pressure. 3.68 g of the methyl ester of N- (5-nitro-1,2,3,4,4-tetrahydro-naphthyl-1 (R, S) -carbonyl) - (L) - methionine are obtained.
  • the filtrate is concentrated to dryness under reduced pressure.
  • the residue is dissolved in 150 cm3 of ethyl acetate and washed with 100 cm3 of an aqueous solution of sodium hydrogencarbonate at 10% (w / v), 100 cm3 of an aqueous solution of citric acid at 10 % (w / v), 100 cm3 of distilled water, another 100 cm3 of an aqueous solution of sodium hydrogenocrbonate at 10% (w / v), and finally per 100 cm3 of a saturated aqueous solution of sodium chloride sodium.
  • the organic phase is dried over magnesium sulfate, filtered and concentrated to dryness under reduced pressure.
  • the farnesyltransferase activity is determined by the amount of ( ⁇ H) farnesyl transferred from ( ⁇ H) farnesylpyrophosphate [( ⁇ H) FPP) to the p21 H-Ras protein.
  • the standard reaction mixture is composed, for a final volume of 60 ⁇ l, of 50 mM Tris-HCl, 5 mM MgCl2, 5 mM dithiotreitol, octyl- ⁇ -D-glucopyranoside 0.2, p21 H-ras 200 picomoles, ( 3 H) FPP (at 61,000 dpm / picomole) 4.5 picomoles.
  • the reaction is initiated by the addition of approximately 5 ng of human farnesyltransferase purified from cultures of THP1 cells. After incubation for 20 minutes at 37 ° C. in a microtiter plate containing 96 holes of 1 cm 3 per plate (Titer Plate®, Beckman), the reaction is stopped by adding 0.4 cm 3 of 0.1% SDS in methanol. at 0 ° C. 0.4 cm 3 of trichloroacetic acid (TCA) 30% in methanol is then added to the mixture. The plates are left for 1 hour in ice.
  • TCA trichloroacetic acid
  • the precipitated content is then retained on a fiberglass membrane Filtermat®, Pharmacia) with the filtration unit (Combi Cell Harvester®, Skatron) and rinsed with 6% trichloroacetic acid in distilled water.
  • the membranes are dried in the microwave oven then impregnated with scintillant by melting under hot air of Meltilex® OPharmacia) and finally counted in cpm in a ⁇ -Plate® counter (LKB). Each test is repeated 3 times.
  • the activity unit is defined by 1 picomole of (-1H) FPP transferred to p21 H-Ras in 20 minutes.
  • the inhibition percentages are obtained by comparison of the tests with and without inhibitor after deduction of the blanks, the IC50 being measured from the inhibitions obtained with 9 different concentrations using the Enzfitter® or Grafit® software.
  • Cell activity can be determined as follows:
  • the cell line is the THAC line (CCL 39 cells transfected with activated Ha-Ras) according to K. Seu en et al., EMBO J., 2 (1) 161-168 (1988).
  • the cells are seeded in 6 cm diameter petri dishes containing DMEM medium, 5% fetal calf serum, 1% G418. After 24 hours of culture, the culture medium is changed (with or without serum) and the product to be studied is added in solution in dimethylformamide (DMF), in the presence or in the absence of DTT (final concentrations of 0.5% in DMF and O.lmM in DTT).
  • DMF dimethylformamide
  • the lysates are clarified by centrifugation at 4,000 rpm for 10 minutes. Extraction with Triton XI 14 makes it possible to separate the farnesylated Ras protein from the non-famesylated Ras protein [C. BORDIER, J. Biol. Chem., 25â (4), 1604-1607 (1981)].
  • the famesylated Ras protein which is more hydrophobic, is found in the detergent phase while the non-famesylated Ras protein is found in the aqueous phase.
  • the samples are denatured by heating to 95 ° C. in denaturation buffer for electrophoresis and deposited on a 14% polyacrylamide gel. When the dye reaches the bottom of the gel, the proteins in the gel are transferred to a PVDF membrane.
  • the Ras protein is revealed by the Western blot technique: the membrane is incubated with a specific anti-Ras monoclonal antibody (pan-Ras Ab3, Oncogene Science) then with protein A labeled at 125 d. After autoradiography, the bands are identified, cut and counted in a ⁇ counter. The radioactivity of the bands corresponding to famesylated Ras and to non-famesylated Ras makes it possible to determine the percentage inhibition of famesylation of the Ras protein. The results obtained are collated in Table I. TABLE I
  • the new products of general formula (I) can be in the form of non-toxic and pharmaceutically acceptable salts.
  • These non-toxic salts include the salts with mineral acids (hydrochloric, sulfuric, hydrobromic, phosphoric, nitric) or with organic acids (acetic, propionic, succinic, maleic, hydroxymaleic, benzoic, fumaric, methanesulfonic, trifluoroacetic or oxalic acids) or with mineral bases (soda, potash, lithine, lime) or organic bases (tertiary amines such as triethylamine, piperidine, benzylamine) depending on the nature of the Rjet R symbols of the product of general formula (I).
  • the present invention also relates to pharmaceutical compositions which contain at least one product of general formula (I) in combination with one or more diluents or pharmaceutically acceptable adjuvants whether inert or physiologically active.
  • compositions can be administered orally, parenterally or rectally.
  • compositions for oral administration include tablets, pills, powders or granules.
  • the active product according to the invention is mixed with one or more inert diluents such as sucrose, lactose or starch.
  • these compositions can include substances other than diluents, for example a lubricant such as magnesium stearate.
  • compositions for oral administration can be used pharmaceutically acceptable emulsions, solutions, suspensions, syrups, elixirs containing inert diluents such as water or paraffin oil. These compositions can also include substances other than diluents, for example wetting, sweetening or flavoring products.
  • the compositions according to the invention for parenteral administration can be sterile aqueous or non-aqueous solutions, suspensions or emulsions.
  • solvent or vehicle propylene glycol, a polyethylene glycol, vegetable oils, in particular olive oil or injectable organic esters, for example ethyl oleate, can be used.
  • compositions can also contain adjuvants, in particular wetting agents, emulsifiers and dispersants. Sterilization can be done in several ways, for example using a bacteriological filter, incorporating sterilizing agents into the composition or by heating. They can also be prepared in the form of sterile solid compositions which can be dissolved at the time of use in sterile water or any other sterile injectable medium.
  • compositions for rectal administration are suppositories which may contain, in addition to the active product, excipients such as cocoa butter.
  • compositions according to the invention are particularly useful in human therapy in the treatment of cancers of various origins.
  • the doses depend on the desired effect, on the duration of the treatment and on the factors specific to the subject to be treated.
  • the doses are, in humans, between 0.1 and 20 mg / kg per day intraperitoneally.
  • the following example illustrates a composition according to the invention.
  • Example 2 200 mg of the product obtained in Example 1 are dissolved in 100 cm 3 of physiological saline. The solution obtained is aseptically distributed in 10 cm 3 ampoules. The ampoules are administered as a single injection or as an infusion.

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Abstract

Novel farnesyl transferase inhibitors of general formula (I), preparation thereof and pharmaceutical compositions containing same. In general formula (I), R1 is Y-S-A1 (where Y is a hydrogen atom, an amino acid residue, a fatty acid residue, or an alkyl or alkoxycarbonyl radical, and A1 is a C1-4 alkylene radical optionally α-substituted in the ⊃C(X1)(Y1) grouping by an amino, alkylamino, alkanoylamino or alkoxycarbonylamino radical wherein the alkyl or alkanoyl portion contains 1-6 carbon atoms; each of X1 and Y1 is a hydrogen atom or X1 and Y1, taken together with the carbon atom to which they are attached, form a ⊃C=O grouping; R'1 is hydrogen or a C1-6 alkyl radical; X is an oxygen or sulphur atom; R2 is a C1-6 alkyl, alkenyl or alkynyl radical optionally substituted by hydroxy, alkoxy, mercapto, alkylthio, alkylsulphinyl or alkylsulphonyl, with the proviso that when R2 is an alkyl radical substituted by a hydroxy radical, then R2 and the α carboxy radical can form a lactone; R'2 is hydrogen or a C1-6 alkyl radical; and R is a hydrogen atom, an optionally substituted alkyl radical or an optionally substituted phenyl radical. Said novel products have anticancer properties.

Description

NOUVEAUX INHIBITEURS DE FARNESYL TRANSFERASE. LEUR NOVEL FARNESYL TRANSFERASE INHIBITORS. THEIR
PREPARATION ET LES COMPOSITIONS PHARMACEUTIQUESPREPARATION AND PHARMACEUTICAL COMPOSITIONS
QUI LES CONTIENNENTWHO CONTAIN THEM
La présente invention concerne de nouveaux inhibiteurs de farnesyl transfé- rase de formule générale :The present invention relates to new farnesyl transferase inhibitors of general formula:
éventuellement leurs sels, leur préparation et les compositions pharmaceutiques qui les contiennent. optionally their salts, their preparation and the pharmaceutical compositions containing them.
L'inhibition de la farnesyl transférase et, par conséquent, de la famésylation de la protéine Ras, bloque la capacité de la protéine Ras mutée à transformer les cellules normales en cellules cancéreuses.Inhibition of farnesyl transferase and, therefore, famesylation of the Ras protein, blocks the ability of the mutated Ras protein to transform normal cells into cancer cells.
La séquence C-terminale du gène Ras contient le motif "CAAX" ou "Cys- Aaaι-Aaa2-XaaM dans lequel Aaa représente un aminoacide aliphatique et Xaa représente un aminoacide quelconque. II est connu que des tétrapeptides avec une séquence CAAX peuvent inhiber la famésylation de la protéine Ras. Par exemple, dans la demande PCT WO 91/16340 et dans la demande EP 0 461 869 ont été décrits des peptides inhibiteurs de la famésyl transférase Cys-Aaaj-Aaa2-Xaa qui sont particulièrement représentés par les peptides Cys-Val-Leu-Ser, Cys-Val-Ile-Met et Cys-Val-Val-Met qui manifestent leur activité inhibitrice à des concentrations voisines de 10"^ ou de 10~?M.The C-terminal sequence of the Ras gene contains the motif "CAAX" or "Cys-Aaaι-Aaa2-Xaa M in which Aaa represents an aliphatic amino acid and Xaa represents any amino acid. It is known that tetrapeptides with a CAAX sequence can inhibit famesylation of the Ras protein. For example, in PCT application WO 91/16340 and in application EP 0 461 869, peptides inhibitors of famesyl transferase Cys-Aaaj-Aaa2-Xaa have been described which are particularly represented by the peptides Cys-Val-Leu-Ser, Cys-Val-Ile-Met and Cys-Val-Val-Met which show their inhibitory activity at concentrations close to 10 "^ or 10 ~ ? M.
Il a maintenant été trouvé, et c'est ce qui fait l'objet de la présente invention, que les peptides de formule générale (I) manifestent leur activité inhibitrice (IC50) à des concentrations de l'ordre de 10"^ ou de 10"^M. Dans la formule générale (I), Ri représente un radical de formule générale Y-S-A^- dans lequel Y représente un atome d'hydrogène, ou un reste d'aminoacide ou un reste d'acide gras ou un radical alkyle ou alkoxycarbonyle, et Aj représente un radical alcoylène droit ou ramifié contenant 1 à 4 atomes de carbone éventuellement substitué en α du groupement >C(Xι)(Yj) par un radical amino, alkylamino, alkanoylamino, alkoxycarbonylamino dont la partie alkyle ou alkanoyle droite ou ramifiée contient 1 à 6 atomes de carbone, X et Yj représentent chacun un atome d'hydrogène ou forment ensemble avec l'atome de carbone auquel ils sont liés un groupement >C=O, R'j représente un atome d'hydrogène ou un radical alkyle droit ou ramifié contenant 1 à 6 atomes de carbone,It has now been found, and this is the subject of the present invention, that the peptides of general formula (I) manifest their inhibitory activity (IC50) at concentrations of the order of 10 "^ or 10 "^ M. In the general formula (I), Ri represents a radical of general formula YSA ^ - in which Y represents a hydrogen atom, or an amino acid residue or a fatty acid residue or an alkyl or alkoxycarbonyl radical, and Aj represents a straight or branched alkylene radical containing 1 to 4 carbon atoms optionally substituted at α of the group > C (Xι) (Yj) by an amino, alkylamino, alkanoylamino, alkoxycarbonylamino radical in which the straight or branched alkyl or alkanoyl part contains 1 to 6 carbon atoms, X and Yj each represent a hydrogen atom or form together with l 'carbon atom to which they are linked a group> C = O, R'j represents a hydrogen atom or a straight or branched alkyl radical containing 1 to 6 carbon atoms,
X représente un atome d'oxygène ou de soufre,X represents an oxygen or sulfur atom,
R2 représente un radical alkyle, alkényle ou alkynyle droit ou ramifié contenant 1 à 6 atomes de carbone éventuellement substitué par un radical hydroxy, alkoxy contenant 1 à 4 atomes de carbone, mercapto, alkylthio contenant 1 à 4 atomes de carbone, alkylsulfinyle contenant 1 à 4 atomes de carbone ou alkylsulfonyle contenant 1 à 4 atomes de carbone, étant entendu que, lorsque R2 représente un radical alkyle substitué par un radical hydroxy, R2 peut former avec le radical carboxy en α une lactone, R'2 représente un atome d'hydrogène ou un radical alkyle droit ou ramifié contenant 1 à 6 atomes de carbone, etR2 represents an alkyl, alkenyl or straight or branched alkynyl radical containing 1 to 6 carbon atoms optionally substituted by a hydroxy radical, alkoxy containing 1 to 4 carbon atoms, mercapto, alkylthio containing 1 to 4 carbon atoms, alkylsulfinyl containing 1 to 4 carbon atoms or alkylsulfonyl containing 1 to 4 carbon atoms, it being understood that, when R2 represents an alkyl radical substituted by a hydroxy radical, R2 can form with the carboxy radical in α a lactone, R'2 represents an atom of hydrogen or a straight or branched alkyl radical containing 1 to 6 carbon atoms, and
R représente un atome d'hydrogène ou un radical alcoyle contenant 1 à 6 atomes de carbone éventuellement substitué par un radical alcoxy contenant 1 à 4 atomes de carbone, alcoylthio contenant 1 à 4 atomes de carbone, alkylsulfinyle contenant 1 à 4 atomes de carbone, alkylsulfonyle contenant 1 à 4 atomes de carbone, phényle, phénoxy, phénylthio, phénylsulf nyl, phénylsulfonyl, alcoylamino contenant 1 à 4 atomes de carbone, dialcoylamino dont chaque partie alcoyle contient 1 à 4 atomes de carbone, ou un radical phényle éventuellement substitué par un ou plusieurs atomes ou radicaux choisis parmi les atomes d'halogène et les radicaux alcoyles, alcoyloxy, alcoylthio ou alcanoyle.R represents a hydrogen atom or an alkyl radical containing 1 to 6 carbon atoms optionally substituted by an alkoxy radical containing 1 to 4 carbon atoms, alkylthio containing 1 to 4 carbon atoms, alkylsulfinyl containing 1 to 4 carbon atoms, alkylsulfonyl containing 1 to 4 carbon atoms, phenyl, phenoxy, phenylthio, phenylsulf nyl, phenylsulfonyl, alkylamino containing 1 to 4 carbon atoms, dialkoylamino each alkyl part of which contains 1 to 4 carbon atoms, or a phenyl radical optionally substituted by a or several atoms or radicals chosen from halogen atoms and alkyl, alkyloxy, alkylthio or alkanoyl radicals.
Plus particulièrement, Rj représente un radical de formule Y-S-Aj- dans lequel Y représente un atome d'hydrogène ou un reste lysine ou un reste d'acide gras contenant jusqu'à 20 atomes de carbone et A représente un radical éthylène ou propylène éventuellement substitué par un radical amino,More particularly, Rj represents a radical of formula YS-Aj- in which Y represents a hydrogen atom or a lysine residue or a fatty acid residue containing up to 20 carbon atoms and A represents an ethylene or propylene radical optionally substituted by an amino radical,
XI et Yl représentent chacun un atome d'hydrogène ou forment ensemble avec l'atome de carbone auquel ils sont liés un groupement >C=O,XI and Yl each represent a hydrogen atom or form together with the carbon atom to which they are linked a group> C = O,
R'i représente un atome d'hydrogène ou un radical méth le, X représente un atome d'oxygène, R2 représente un radical alkyle contenant 1 à 4 atomes de carbone éventuellement substitué par un radical hydroxy, méthoxy, mercapto, méthylthio, mémylsulfinyle ou méthylsulfonyle,R'i represents a hydrogen atom or a methyl radical, X represents an oxygen atom, R2 represents an alkyl radical containing 1 to 4 carbon atoms optionally substituted by a hydroxy, methoxy, mercapto, methylthio, memylsulfinyl or methylsulfonyl radical,
R'2 représente un atome d'hydrogène ou un radical méthyle, et R représente un atome d'hydrogène ou un radical alcoyle contenant 1 à 4 atomes de carbone, éventuellement substitué par un radical alcoxy, ou un radical phényle. Plus particulièrement encore,R'2 represents a hydrogen atom or a methyl radical, and R represents a hydrogen atom or an alkyl radical containing 1 to 4 carbon atoms, optionally substituted by an alkoxy radical, or a phenyl radical. More particularly still,
Rj représente un radical de formule Y-S-Aj- dans lequel Y représente un atome d'hydrogène et Aj représente un radical éthylène ou propylène éventuellement substitué par un radical amino,Rj represents a radical of formula Y-S-Aj- in which Y represents a hydrogen atom and Aj represents an ethylene or propylene radical optionally substituted by an amino radical,
Xl et Yl représentent chacun un atome d'hydrogène ou forment ensemble avec l'atome de carbone auquel ils sont liés un groupement >C=O,Xl and Yl each represent a hydrogen atom or form together with the carbon atom to which they are linked a group> C = O,
R'i représente un atome d'hydrogène,R'i represents a hydrogen atom,
X représente un atome d'oxygène, R2 représente un radical méthyle, éthyle, propyle ou butyle éventuellement substitué par un radical hydroxy, méthoxy, mercapto ou méthylthio,X represents an oxygen atom, R2 represents a methyl, ethyl, propyl or butyl radical optionally substituted by a hydroxy, methoxy, mercapto or methylthio radical,
R'2 représente un atome d'hydrogène, etR'2 represents a hydrogen atom, and
R représente un atome d'hydrogène ou un radical alcoyle contenant 1 à 4 atomes de carbone. Tout particulièrement intéressants sont les produits de formule générale (I) dans laquelle Rj représente un radical mercapto-2 éthyle ou amino-1 mercapto-2 éthyle, X et Yj représentent chacun un atome d'hydrogène ou forment ensemble avec l'atome de carbone auquel ils sont liés un groupement >C=O, R'j représente un atome d'hydrogène, X représente un atome d'oxygène, R2 représente un radical n.butyle ou méthylthio-2 éthyle et R'2 représente un atome d'hydrogène, et R représente un atome d'hydrogène ou un radical méthyle.R represents a hydrogen atom or an alkyl radical containing 1 to 4 carbon atoms. Very particularly interesting are the products of general formula (I) in which Rj represents a 2-mercapto-ethyl or 1-amino-2-mercapto-ethyl radical, X and Yj each represent a hydrogen atom or form together with the carbon atom to which they are linked a group> C = O, R'j represents a hydrogen atom, X represents an oxygen atom, R2 represents an n.butyl or 2-methylthio-ethyl radical and R'2 represents a hydrogen, and R represents a hydrogen atom or a methyl radical.
La présente invention concerne également les formes stéréoisomères des produits de formule générale (I). Les restes des aminoacides représentés parThe present invention also relates to the stereoisomeric forms of the products of general formula (I). The remains of the amino acids represented by
Rι C(Xι)(Yι)(NR'ι) et R'2CH(NR'2)CO-OH ont de préférence la configuration des aminoacides naturels.Rι C (Xι) (Yι) (NR'ι) and R'2CH (NR'2) CO-OH preferably have the configuration of natural amino acids.
La présente invention concerne également les sels minéraux ou organiques des produits de formule générale (I).The present invention also relates to the mineral or organic salts of the products of general formula (I).
Les nouveaux produits selon l'invention peuvent être préparés par application des méthodes connues dérivées des méthodes utilisées plus particulièrement en chimie peptidique pour l'assemblage de chaînes. Généralement, les produits de formule générale (I), laquelle Xj et Yj forment ensemble avec l'atome de carbone auquel ils sont liés un groupement >C=O et X représente un atome d'oxygène sont obtenus à partir de l'acide 5-nitro-l,2,3,4- tétrahydro-naphtyl-1-carboxylique sur lequel est condensé un aminoacide de formule générale :The new products according to the invention can be prepared by applying known methods derived from the methods used more particularly in peptide chemistry for the assembly of chains. Generally, the products of general formula (I), which Xj and Yj form together with the carbon atom to which they are bonded a group> C = O and X represents an oxygen atom are obtained from acid 5 -nitro-1,2,3,4- tetrahydro-naphthyl-1-carboxylic on which is condensed an amino acid of general formula:
R',R ',
HN COOR' (II)HN COOR '(II)
dans laquelle R2 et R'2 sont définis comme précédemment et R' représente un radical alcoyle contenant 1 à 4 atomes de carbone, éventuellement substitué par un radical phényle, de préférence un radical tert-butyle, en opérant en présence d'un agent de condensation, tel que hydroxybenzotriazole et le dicyclohexylcarbodϋmide, et d'une base, telle que la triéthylamine, dans un solvant organique, tel que le diméthyl- formamide, pour donner un produit de formule générale : in which R2 and R'2 are defined as above and R 'represents an alkyl radical containing 1 to 4 carbon atoms, optionally substituted by a phenyl radical, preferably a tert-butyl radical, operating in the presence of an agent condensation, such as hydroxybenzotriazole and dicyclohexylcarbodϋmide, and of a base, such as triethylamine, in an organic solvent, such as dimethylformamide, to give a product of general formula:
dans laquelle X représente un atome d'oxygène, R2, R'2 et R' sont définis comme précédemment qui est réduit, de préférence au moyen de chlorure stanneux, en produit de formule générale : in which X represents an oxygen atom, R2, R'2 and R 'are defined as above which is reduced, preferably by means of stannous chloride, to the product of general formula:
dans laquelle X représente un atome d'oxygène, R2, R'2 et R' sont définis comme précédemment, sur lequel est condensé un produit de formule générale : in which X represents an oxygen atom, R2, R'2 and R ' are defined as above, on which a product of general formula is condensed:
(V) x γ. dans laquelle Rj est défini comme précédemment et Xj et Yj forment ensemble avec l'atome de carbone auquel ils sont liés un groupement >C=O, étant entendu que les fonctions amino et mercapto portées par Rj sont éventuellement protégées par des groupements protecteurs appropriés tel qu'un radical trityle pour la fonction mercapto ou un radical tert-butoxycarbonyle pour la fonction amino, en opérant de préférence en présence d'un halogénoformiate d'alcoyle (chloroformiate d'isobutyle) et d'une base organique (N-méthylmorpholine) dans un solvant organique inerte (têtrahydrofuranne) pour obtenir un produit de formule générale :(V) x γ. in which Rj is defined as above and Xj and Yj form together with the carbon atom to which they are linked a group> C = O, it being understood that the amino and mercapto functions carried by Rj are optionally protected by appropriate protective groups such than a trityl radical for the mercapto function or a tert-butoxycarbonyl radical for the amino function, preferably operating in the presence of an alkyl haloformate (isobutyl chloroformate) and an organic base (N-methylmorpholine) in an inert organic solvent (tetrahydrofuran) to obtain a product of general formula:
XlχYl dans laquelle les symboles X, Xj, Y\, Rj, R2, R'2 et R1 sont définis comme précédemment dont les groupements protecteurs sont remplacés par des atomes d'hydrogène, au moyen d'acide trifluoroacétique en présence d'éthaneditiol, lorsque les groupements protecteurs représentent des radicaux trityle, tert-butoxycarbonyle ou tert-butyle, pour obtenir un produit de formule générale (I) dans laquelle Xj et Yj forment ensemble avec l'atome de carbone auquel ils sont liés un groupement >C=O. Généralement les produits de formule générale (I) dans laquelle les symboles Xj et Y représentent chacun un atome d'hydrogène peuvent être obtenus par action d'un aldéhyde de formule générale : Xl χ Yl in which the symbols X, Xj, Y \, Rj, R2, R'2 and R 1 are defined as above, the protective groups of which are replaced by hydrogen atoms, by means of trifluoroacetic acid in the presence of 'ethaneditiol, when the protective groups represent trityl, tert-butoxycarbonyl or tert-butyl radicals, to obtain a product of general formula (I) in which Xj and Yj form together with the carbon atom to which they are linked a group> C = O. Generally the products of general formula (I) in which the symbols Xj and Y each represent a hydrogen atom can be obtained by the action of an aldehyde of general formula:
Ri-CHO (VII) dans laquelle Rj est défini comme précédemment, étant entendu que les fonctions amino et mercapto portées par Rj sont éventuellement protégées par des groupements protecteurs appropriés tel qu'un radical trityle pour la fonction mercapto ou un radical tert-butoxycarbonyle pour la fonction amino, sur un produit de formule générale (V) en présence d'un agent réducteur tel que le cyanoborohydrure de sodium, le borohydrure de sodium, le triacétoxyborohydrure de sodium ou l'hydrogène en présence d'un catalyseur. Généralement la réaction s'effectue dans un solvant organique tel qu'un alcool comme le méthanol éventuellement en association avec un autre solvant organique tel qu'un éther comme le tétrahydrofuranne. Il est particulière¬ ment avantageux d'opérer en milieu anhydre.Ri-CHO (VII) in which Rj is defined as above, it being understood that the amino and mercapto functions carried by Rj are optionally protected by appropriate protective groups such as a trityl radical for the mercapto function or a tert-butoxycarbonyl radical for the amino function, on a product of general formula (V) in the presence of a reducing agent such as sodium cyanoborohydride, sodium borohydride, sodium triacetoxyborohydride or hydrogen in the presence of a catalyst. Generally the reaction is carried out in an organic solvent such as an alcohol such as methanol optionally in combination with a other organic solvent such as an ether such as tetrahydrofuran. It is particularly advantageous to operate in an anhydrous medium.
La condensation de l'aldéhyde avec l'aminé étant réalisée, les groupements protecteurs sont remplacés par des atomes d'hydrogène par application des techniques habituelles. Ainsi, les groupements protecteurs Boc ou trityle ou tert-butyle peuvent être remplacés par des atomes d'hydrogène au moyen d'acide trifluoroacétique en présence d'éthanedithiol.The condensation of the aldehyde with the amine being carried out, the protective groups are replaced by hydrogen atoms by application of the usual techniques. Thus, the Boc or trityl or tert-butyl protecting groups can be replaced by hydrogen atoms by means of trifluoroacetic acid in the presence of ethanedithiol.
Généralement, les produits de formule générale (I) dans laquelle X représente un atome de soufre peuvent être obtenus à partir d'un produit de formule générale (III) dans laquelle X représente un atome d'oxygène par thionation puis en effectuant les étapes de réduction, de condensation ou d'amination réductrice selon le cas et de déprotection décrites précédemment pour la préparation d'un produit de formule générale (I) dans laquelle X représente un atome d'oxygène.Generally, the products of general formula (I) in which X represents a sulfur atom can be obtained from a product of general formula (III) in which X represents an oxygen atom by thionation then by performing the steps of reduction, condensation or reductive amination as appropriate and deprotection described above for the preparation of a product of general formula (I) in which X represents an oxygen atom.
Lorsque dans la formule générale (I) le symbole R2 forme avec la fonction carboxy en α une lactone, le traitement en milieu basique du produit correspondant conduit au produit de formule générale (I) dans laquelle R2 représente un radical alkyle substitué par un radical hydroxy. Généralement, l'ouverture de la lactone s'effectue dès que le pH devient supérieur à 7. Il est particulièrement avantageux d'opérer en présence d'une base minérale (soude, potasse) en milieu hydro-alcoolique tel qu'un mélange eau-méthanol.When in the general formula (I) the symbol R2 forms with the carboxy function in α a lactone, the treatment in basic medium of the corresponding product leads to the product of general formula (I) in which R2 represents an alkyl radical substituted by a hydroxy radical . Generally, the lactone is opened as soon as the pH becomes greater than 7. It is particularly advantageous to operate in the presence of a mineral base (soda, potash) in a hydro-alcoholic medium such as a water mixture. -methanol.
Les produits de formule générale (I) dans laquelle R représente un radical alcoyle éventuellement substitué ou un radical phényle éventuellement substitué comme indiqué précédemment peuvent être obtenus par estérification d'un produit de formule générale (I) dans laquelle R représente un atome d'hydrogène dans les conditions habituelles d'estérification qui ne touchent pas au reste de la molécule.The products of general formula (I) in which R represents an optionally substituted alkyl radical or an optionally substituted phenyl radical as indicated above can be obtained by esterification of a product of general formula (I) in which R represents a hydrogen atom under the usual esterification conditions which do not affect the rest of the molecule.
Les produits de formule générale (I) dans laquelle R représente un atome d'hydrogène peuvent aussi être obtenus par saponification d'un produit de formule générale (VI) suivi du remplacement des groupements protecteurs portés par R\ dans les conditions décrites précédemment. L'acide 5-nitro-l,2,3,4-tétrahydro-naphtyl-l-carboxylique, qui peut être obtenu en mélange avec l'acide 7-nitro-l,2,3,4-tétrahydro-naphtyl-l-carboxylique, peut être préparé selon le procédé décrit par T. NAKAYAMA et coll., Chem. Pharm. Bull., 32, 3968 (1984).The products of general formula (I) in which R represents a hydrogen atom can also be obtained by saponification of a product of general formula (VI) followed by the replacement of the protective groups carried by R \ under the conditions described above. 5-nitro-1,2,3,4-tetrahydro-naphthyl-l-carboxylic acid, which can be obtained as a mixture with 7-nitro-1,2,3,4-tetrahydro-naphthyl-l acid -carboxylic, can be prepared according to the process described by T. NAKAYAMA et al., Chem. Pharm. Bull., 32, 3968 (1984).
Les produits de formule générale (I) peuvent être purifiés selon les méthodes habituelles telles que la chromatographie. Les exemples suivants illustrent la préparation des produits selon l'invention.The products of general formula (I) can be purified according to the usual methods such as chromatography. The following examples illustrate the preparation of the products according to the invention.
EXEMPLE 1EXAMPLE 1
On prépare l'acide 5-nitro-l,2,3,4-tétrahydro-naphtyl-l(R,S)-carboxylique selon la méthode de NAKAYAMA, T et coll., Chem. Pharm. Bull., 3.2, 3968 1984). A une solution de 1,23 g d'acide 5-nitro-l,2,3,4-tétrahydro-naphtyl-l(R,S)- carboxylique dans 25 cm3 de chloroforme et 7,5 cm3 de diméthylformamide, on ajoute 1,24 g de chlorhydrate de l'ester méthylique de la (L)-méthionine, 0,84 g de 1-hydroxybenzotriazole, 0,9 cm3 de i-riéthylamine et 1,28 g de dicyclohexyl- carbodiimide. Le mélange réactiormel est agité pendant 2 jours à une température voisine de 20°C, filtré sur verre fritte, puis lavé par 50 cm3 de chloroforme. La solution organique est lavée 2 fois par 50 cm3 d'une solution aqueuse d'hydrogénocarbonate de sodium à 10 % (p/v) puis une fois par 50 cm3 d'une solution aqueuse d'acide citrique à 10 %, une fois par de l'eau distillé, et enfin par une solution aqueuse saturée de chlorure de sodium. La phase organique est séchée sur du sulfate de magnésium, filtrée puis concentrée à sec sous pression réduite. On obtient 3,68 g de l'ester méthylique de la N-(5-nitro-l,2,3,4-tétrahydro-naphtyl-l(R,S)-carbonyl)-(L)- méthionine sous forme d'une huile dont les caractéristiques sont les suivantes : -spectre de résonance magnétique nucléaire du proton (300 MHz ; (CD3)2SO d6 ; δ en ppm ; constantes de couplage J en Hz) : 1,55-2.10 (mt, 6H : CH2CH2 en 2 et CH2 en 3) ; 2,07 (s, 3H : SCH3) ; 2,56 (mt, 1H : CH en 1) ; 4,45 (mt, 1H : CHCOO) ; 7,35-7,55 et 7,77 ( 2 mis, respectivement 2H et 1H : H en 6 et H en 7 et H en 8) ; 8,63 et 8,69 (2 d, J = 8,5, 1H : CONH).5-Nitro-1,2,3,4-tetrahydro-naphthyl-1 (R, S) -carboxylic acid is prepared according to the method of NAKAYAMA, T et al., Chem. Pharm. Bull., 3.2, 3968 1984). To a solution of 1.23 g of 5-nitro-1,2,3,4,4-tetrahydro-naphthyl-1 (R, S) - carboxylic acid in 25 cm3 of chloroform and 7.5 cm3 of dimethylformamide, 1.24 g of (L) -methionine methyl ester hydrochloride, 0.84 g of 1-hydroxybenzotriazole, 0.9 cm3 of i-riethylamine and 1.28 g of dicyclohexylcarbodiimide. The reaction mixture is stirred for 2 days at a temperature in the region of 20 ° C., filtered through sintered glass, then washed with 50 cm 3 of chloroform. The organic solution is washed twice with 50 cm3 of an aqueous solution of sodium hydrogencarbonate at 10% (w / v) then once with 50 cm3 of an aqueous solution of citric acid at 10%, once distilled water, and finally with a saturated aqueous solution of sodium chloride. The organic phase is dried over magnesium sulfate, filtered and then concentrated to dryness under reduced pressure. 3.68 g of the methyl ester of N- (5-nitro-1,2,3,4,4-tetrahydro-naphthyl-1 (R, S) -carbonyl) - (L) - methionine are obtained. '' an oil whose characteristics are as follows: - nuclear magnetic resonance spectrum of the proton (300 MHz; (CD3) 2SO d6; δ in ppm; coupling constants J in Hz): 1.55-2.10 (mt, 6H: CH2CH2 in 2 and CH2 in 3); 2.07 (s, 3H: SCH3); 2.56 (mt, 1H: CH in 1); 4.45 (mt, 1H: CHCOO); 7.35-7.55 and 7.77 (2 mis, respectively 2H and 1H: H in 6 and H in 7 and H in 8); 8.63 and 8.69 (2 d, J = 8.5, 1H: CONH).
A une solution de 3,68 g d'ester méthylique de la N-(5-nitro-l,2,3,4- tétrahydro-naphthyl-l(R,S)-carbonyl)-(L)-méthionine dans 100 cm3 d'éthanol, on ajoute 6,32 g de dihydrate de chlorure d'étain (II). Le mélange réactionnel est agité pendant 30 minutes à une température voisine de 70°C puis refroidi à une température voisine de 20°C. Après dilution par 100 cm3 d'acétate d'éthyle, le mélange réactionnel est versé sur de la glace puis amené à pH = 7-8 par addition d'une solution aqueuse d'hydrogénocarbonate de sodium à 5 %. Le mélange obtenu est filtré sur verre fritte garni de célite. La phase organique est séparée par décantation et la phase aqueuse est extraite 2 fois par 150 cm3 d'acétate d'éthyle. Les phases organiques sont réunies, séchées sur du sulfate de magnésium, filtrées et concentrées à sec sous pression réduite. On obtient ainsi 2,49 g d'ester méthylique de la N-(5-amino-l, 2,3,4- tétrahydro-naphtyl-l(R,S)-carbonyl)-(L)-méthionine sous forme d'une huile dont les caractéristiques sont les suivantes : - spectre de résonance magnétique nucléaire du proton (300 MHz ; (CD3)2SO d6 ; δ en ppm ; constantes de couplage J en Hz) : 1,50-2,10 (mt, 6H : CH2CH2 en 2 et CH3 en 2) ; 2,10 (s, 3H : SCH3) ; 2,39 et 2,53 (2 mts, 2H chacun : CH2S et CH2 en 4) ;To a solution of 3.68 g of methyl ester of N- (5-nitro-1,2,3,4- tetrahydro-naphthyl-1 (R, S) -carbonyl) - (L) -methionine in 100 cm3 of ethanol, 6.32 g of tin (II) chloride dihydrate is added. The reaction mixture is stirred for 30 minutes at a temperature in the region of 70 ° C and then cooled to a temperature in the region of 20 ° C. After dilution with 100 cm3 of ethyl acetate, the reaction mixture is poured onto ice and then brought to pH = 7-8 by addition of a 5% aqueous sodium hydrogen carbonate solution. The mixture obtained is filtered through a sintered glass lined with celite. The organic phase is separated by decantation and the aqueous phase is extracted twice with 150 cm3 of ethyl acetate. The organic phases are combined, dried over magnesium sulfate, filtered and concentrated to dryness under reduced pressure. 2.49 g of N- (5-amino-1,2,3,4,4-tetrahydro-naphthyl-1 (R, S) -carbonyl) - (L) -methionine methyl ester are thus obtained. '' an oil with the following characteristics: - proton nuclear magnetic resonance spectrum (300 MHz; (CD3) 2SO d6; δ in ppm; coupling constants J in Hz): 1.50-2.10 (mt, 6H: CH2CH2 in 2 and CH3 in 2) ; 2.10 (s, 3H: SCH3); 2.39 and 2.53 (2 mts, 2H each: CH2S and CH2 in 4);
3.66 (mt, 1H : CH en 1) ; 3,69 (s, 3H : CHCOO) ; 4,75 (s large, 2H : NH2) ; 6,30- 6,60 et 6,82 (2 mts, respectivement 2H et 1H : H en 6 et H en 7 et H en 8) ; 8,48 (d,3.66 (mt, 1H: CH in 1); 3.69 (s, 3H: CHCOO); 4.75 (br s, 2H: NH2); 6.30-6.60 and 6.82 (2 mts, respectively 2H and 1H: H in 6 and H in 7 and H in 8); 8.48 (d,
J = 9, 1H : CONH).J = 9, 1H: CONH).
A une solution de 1,24 g d'ester méthylique de la N-(5-amino-l,2,3,4- téi-rahydro-naphtyl-l(R,S)-carbonyl)-(L)-méthionine dans 60 cm3 de méthanol, on ajoute 3,73 g de S-triphénylméthyl-N-tert-butoxycarbonyl-cystéinal, 0,48 cm3 d'acide acétique concentré, du tamis moléculaires (3Â) puis 0,52 g de cyanoborohydrure de sodium . Le mélange réactionnel est agité pendant 2 jours à une température voisine de 20°C puis filtré sur verre fritte garni de célite. Le verre fritte est lavé par du méthanol. Le filtrat est concentré à sec sous pression réduite. Le résidu est dissous dans 150 cm3 d'acétate d'éthyle et lavé par 100 cm3 d'une solution aqueuse d'hydrogénocarbonate de sodium à 10 % (p/v), 100 cm3 d'une solution aqueuse d'acide citrique à 10 % (p/v), 100 cm3 d'eau distillé, encore 100 cm3 d'une solution aqueuse d'hydrogénocrbonate de sodium à 10 % (p/v), et enfin par 100 cm3 d'une solution aqueuse saturée de chlorure de sodium. La phase organique est séchée sur du sulfate de magnésium, filtrée et concentrée à sec sous pression réduite. On obtient 5,62 g de l'ester méthylique de la N- [5- (2(R) -tert-butoxycarbonylamino-3-triphénylméthylmercapto-propylamino) - l,2,3,4-tétrahydro-naphtyl-l(R,S)-carbonyl]-(L)-méthionine sous forme d'un meringue beige dont les caractéristiques sont les suivantes :To a solution of 1.24 g of methyl ester of N- (5-amino-1,2,3,4,4-tei-rahydro-naphthyl-1 (R, S) -carbonyl) - (L) -methionine in 60 cm3 of methanol, 3.73 g of S-triphenylmethyl-N-tert-butoxycarbonyl-cysteinal, 0.48 cm3 of concentrated acetic acid, molecular sieve (3A) and then 0.52 g of sodium cyanoborohydride are added . The reaction mixture is stirred for 2 days at a temperature in the region of 20 ° C. and then filtered through sintered glass lined with celite. The sintered glass is washed with methanol. The filtrate is concentrated to dryness under reduced pressure. The residue is dissolved in 150 cm3 of ethyl acetate and washed with 100 cm3 of an aqueous solution of sodium hydrogencarbonate at 10% (w / v), 100 cm3 of an aqueous solution of citric acid at 10 % (w / v), 100 cm3 of distilled water, another 100 cm3 of an aqueous solution of sodium hydrogenocrbonate at 10% (w / v), and finally per 100 cm3 of a saturated aqueous solution of sodium chloride sodium. The organic phase is dried over magnesium sulfate, filtered and concentrated to dryness under reduced pressure. 5.62 g of the methyl ester of N- [5- (2 (R) -tert-butoxycarbonylamino-3-triphenylmethylmercapto-propylamino) - 1,2,3,4-tetrahydro-naphthyl-1 are obtained. , S) -carbonyl] - (L) -methionine in the form of a beige meringue, the characteristics of which are as follows:
- spectre de résonance magnétique nucléaire du proton (300 MHz ; (CD3)2SO d6 avec quelques gouttes de CD3COOD d4 ; δ en ppm ; T = 393 K ; constantes de couplage J en Hz) : 1,38 (s, 9H : C(CH3) ) ; 1,50-2,05 (mt, 6H : CH2CH2 en 2 et CH2 en 3) ; 2,06 (s, 3H : SCH3) ; 2,05-2,45 et 2,53 ( 2 mts, respectivement 4H et 2H: 2 CH S et CH2 en 4) ; 2,85-3,05 (mt, 2H : NH2) ; 3,63 (mt, 1H : CH en 1) ; 3,63 et- proton nuclear magnetic resonance spectrum (300 MHz; (CD3) 2SO d6 with a few drops of CD3COOD d4; δ in ppm; T = 393 K; coupling constants J in Hz): 1.38 (s, 9H: C (CH 3 )); 1.50-2.05 (mt, 6H: CH 2 CH 2 in 2 and CH2 in 3); 2.06 (s, 3H: SCH3); 2.05-2.45 and 2.53 (2 mts, respectively 4H and 2H: 2 CH S and CH2 in 4); 2.85-3.05 (mt, 2H: NH 2 ); 3.63 (mt, 1H: CH in 1); 3.63 and
3.67 (2 s, 3H : COOCH3) ; 3,71 (mt, 1H : CHN) ; 4,43 (mt, 1H : CHCOO) ; 6,30- 6,56 et 6,90 (2 mts, respectivement 2H et 1H : H en 6 et H en 7 et H en 8) ; 7,15-7,35 (mt, 15H : aromatiques) ; 8,36 et 8,39 (2 d résiduels, J = 10, 1H : CONH).3.67 (2 s, 3H: COOCH3); 3.71 (mt, 1H: CHN); 4.43 (mt, 1H: CHCOO); 6.30-6.56 and 6.90 (2 mts, respectively 2H and 1H: H in 6 and H in 7 and H in 8); 7.15-7.35 (mt, 15H: aromatics); 8.36 and 8.39 (residual 2 d, J = 10, 1H: CONH).
A un mélange de 5,45 g de l'ester méthylique de N-[5-(2(R)-tert- butoxycarbonylamino- 3-triphénylméthylmercapto-propylamino) - 1 ,2 ,3 ,4-tétrahydro- naphtyl-l(R,S)-carbonyl]-(L)-méthionine dans 10 cm3 d'éthanedithiol, on ajoute, à une température voisine de 20°C, 110 cm3 d'acide trifluoroacétique. Le mélange réactionnel est agité pendant 2 heures à une température voisine de 20°C, puis concentré sous pression réduite. Le résidu est trituré 3 fois par 25 cm3 d'éther éthylique puis séché sous pression réduite. Le résidu est purifié par chromatographie liquide à haute performance (phase C18) en éluant avec un mélange acétonitrile-eau contenant 0,1 % d'acide trifluoroacétique. On obtient 0,4 g de trifluoroacétate de l'ester méthylique de la N-[5-(2(R)-amino-3-mercapto-propylamino)-l,2,3,4- tétrahydro-naphtyl-l(R,S)-carbonyl]-(L)-méthionine sous forme d'une poudre dont les caractéristiques sont les suivantes :To a mixture of 5.45 g of the methyl ester of N- [5- (2 (R) -tert- butoxycarbonylamino- 3-triphenylmethylmercapto-propylamino) - 1, 2, 3, 4-tetrahydro-naphthyl-1 ( R, S) -carbonyl] - (L) -methionine in 10 cm 3 of ethanedithiol, 110 cm 3 of trifluoroacetic acid are added at a temperature close to 20 ° C. The reaction mixture is stirred for 2 hours at a temperature in the region of 20 ° C., then concentrated under reduced pressure. The residue is triturated 3 times with 25 cm 3 of ethyl ether and then dried under reduced pressure. The residue is purified by high performance liquid chromatography (phase C18), eluting with an acetonitrile-water mixture containing 0.1% trifluoroacetic acid. 0.4 g of trifluoroacetate is obtained from the methyl ester of N- [5- (2 (R) -amino-3-mercapto-propylamino) -1,2,3,4-tetrahydro-naphthyl-1 (R , S) -carbonyl] - (L) -methionine in the form of a powder, the characteristics of which are as follows:
- spectre de résonance magnétique nucléaire du proton (300 MHz ; (CD3)2SO d6 avec quelques gouttes de CD3COOD d4 ; T = 393°K ; δ en ppm ; constantes de couplage J en Hz) : 1,55-2,10 (mt, 6H : CH2CH2 en 2 et CH2 en 3) ; 2,04 (s, 3H : SCH3) ; 2,30-2,65 et 2,80 (2 mts, respectivement 4H et 2H : 2 CH2S et CH2 en 4) ; 3,30 et 3,35-3,50 (respectivement dd, J = 14 et 8 et mt, 1H chacun NCH2) ; 3,43 (mt, 1H : CHN) ; 3,63 (mt, 1H : CH en 1) ; 3,63 (s, 3H : COOCH3) ; 4,42 (mt, 1H : CHCOO) ; 6,40-6,60 et 6,95 (2 mts, respectivement 2H et 1H : H en 6 et H en 7 et H en 8) ; 8,35 et 8,38 (2 d résiduels, J = 8, 1H : CONH).- proton nuclear magnetic resonance spectrum (300 MHz; (CD3) 2SO d6 with a few drops of CD3COOD d4; T = 393 ° K; δ in ppm; coupling constants J in Hz): 1.55-2.10 ( mt, 6H: CH2CH2 at 2 and CH2 at 3); 2.04 (s, 3H: SCH3); 2.30-2.65 and 2.80 (2 mts, respectively 4H and 2H: 2 CH2S and CH2 in 4); 3.30 and 3.35-3.50 (respectively dd, J = 14 and 8 and mt, 1H each NCH2); 3.43 (mt, 1H: CHN); 3.63 (mt, 1H: CH in 1); 3.63 (s, 3H: COOCH3); 4.42 (mt, 1H: CHCOO); 6.40-6.60 and 6.95 (2 mts, respectively 2H and 1H: H in 6 and H in 7 and H in 8); 8.35 and 8.38 (residual 2 d, J = 8, 1H: CONH).
- analyse élémentaire : C20H31N3O3S2, 1,25 CF3CO2H- elementary analysis: C20H31N3O3S2, 1.25 CF3CO2H
Calculé % : C = 47,7 ; H = 5,70 ; N = 7,4 ; S = 11,9 Trouvé : 47,8 ; 5,37 ; 7, 1 ; 10,6Calculated%: C = 47.7; H = 5.70; N = 7.4; S = 11.9 Found: 47.8; 5.37; 7, 1; 10.6
EXEMPLE 2 A une solution de 1,98 g d'ester méthylique de la N-[5-(2(R)-tert- butoxycarbonylamino3-triphénylméΛylmercapto-propylammo)-l,2,3,4-tétrahydro- naphtyl-l(R,S)-carbonyl]-(L)-méthionine dans 8,4 cm3 d'eau distillée et 30 cm3 de tétrahydrofuranne, on ajoute 0,17 g de lithine monohydratée (LiOH, H2O). La solution est agitée pendant 20 heures à une température voisine de 20°C, puis concentrée sous pression réduite. Le résidu est dissous dans de l'eau distillée. Le pH est amené à 3 par addition d'une solution aqueuse d'acide citrique à 10 % p/v). La phase aqueuse est extraite 3 fois par 100 cm3 d'acétate d'éthyle. Les phases organiques sont réunies, lavées avec une solution aqueuse saturée de chlorure de sodium, séchées sur du sulfate de magnésium, filtrées et concentrées à sec sous pression réduite. On obtient 1,92 g de la N-[5-(2(R)-tert-butoxycarbonylamino-3-triphénylméthylmercapto- propylamino)-l,2,3,4-tétrahydro-naphtyl-l (R,S)-carbonyl]-(L)-méthionine sous forme d'une meringue.EXAMPLE 2 To a solution of 1.98 g of methyl ester of N- [5- (2 (R) -tert- butoxycarbonylamino3-triphenylméΛylmercapto-propylammo) -l, 2,3,4-tetrahydro-naphthyl-l ( R, S) -carbonyl] - (L) -methionine in 8.4 cm3 of distilled water and 30 cm3 of tetrahydrofuran, 0.17 g of lithine monohydrate (LiOH, H2O) is added. The solution is stirred for 20 hours at a temperature in the region of 20 ° C., then concentrated under reduced pressure. The residue is dissolved in distilled water. The pH is brought to 3 by addition of an aqueous solution of citric acid at 10% w / v). The aqueous phase is extracted 3 times with 100 cm3 of ethyl acetate. The organic phases are combined, washed with a saturated aqueous solution of sodium chloride, dried over magnesium sulfate, filtered and concentrated to dryness under reduced pressure. 1.92 g of N- [5- (2 (R) -tert-butoxycarbonylamino-3-triphenylmethylmercapto-propylamino) -1, 2,3,4-tetrahydro-naphthyl-1 (R, S) -carbonyl are obtained. ] - (L) -methionine in the form of a meringue.
A un mélange de 0,5 g de N-[5-(2(R)-tert-butoxycarbonylamino-3-triphényl- méthylmercapto-propylamino)-l,2,3,4-tétrahydro-naphtyl-l(R,S)-carbonyl]-(L)- méthionine dans 2,5 cm3 de triéthylsilane, on ajoute, à une température voisine de 20°C, 8 cm3 d'acide trifluoroacétique. Le mélange réactionnel est agité pendant 2 heures à une température voisine de 20°C, puis concentré sous pression réduite . Le résidu est trituré 3 fois par 25 cm3 d'éther éthylique puis séché sous pression réduite. Le résidu est purifié par chromatographie liquide à haute performance (phase Cl 8) en éluant avec un mélange acétonitrile-eau contenant 0,1 % d'acide trifluoroacétique. On obtient 0,094 g de trifluoroacétate de la N-[5-(2(R)-amino-3-mercapto-propylamino) l,2,3,4-tétrahydro-naphtyl-l(R,S)-carbonyl]-(L)-méthionine sous forme d'une poudre dont les caractéristiques sont les suivantes : -spectre de résonance magnétique nucléaire du proton (300 MHz ; (CD3)2SO d6 ; δ en ppm ; constantes de couplage J en Hz) : 1,55-2,10 (mt, 6H : CH2CH2 en 2 et CH2 en 3) ; 2,06 (s, 3H :SCH3) ; 2,30-2,65 et 2,75-2,30 (2 mts, respectivement 4H et 2H: 2 CH2S et CH2 en 4) ; 3,30 et 3,35-3,50 ( respectivement dd, J= 14 et 8 et mt, 1H chacun : NCH2) ; 3,43 (mt, 1H : CHN) ; 3,66 (mt, 1H : CH en 1) ; 4,37 (mt, 1H : CHCOO) ; 6,40-6,60 et 6,95 (2 mts, respectivement 2H et 1H : H en 6 et H en 7 et H en 8) ; 7,95 (s large, 3H : NH3+) ; 8,33 et 8,35 (2 d, J = 9, 1H : CONH). - analyse élémentaire : C19H29N3O3S2. 1,33 CF3CO2HTo a mixture of 0.5 g of N- [5- (2 (R) -tert-butoxycarbonylamino-3-triphenyl-methylmercapto-propylamino) -l, 2,3,4-tetrahydro-naphthyl-l (R, S ) -carbonyl] - (L) - methionine in 2.5 cm3 of triethylsilane, 8 cm3 of trifluoroacetic acid is added, at a temperature in the region of 20 ° C. The reaction mixture is stirred for 2 hours at a temperature in the region of 20 ° C., then concentrated under reduced pressure. The residue is triturated 3 times with 25 cm 3 of ethyl ether and then dried under reduced pressure. The residue is purified by high performance liquid chromatography (phase Cl 8), eluting with an acetonitrile-water mixture containing 0.1% trifluoroacetic acid. 0.094 g of trifluoroacetate of N- [5- (2 (R) -amino-3-mercapto-propylamino) 1,2,3,4-tetrahydro-naphthyl-1 (R, S) -carbonyl] - is obtained L) -methionine in the form of a powder, the characteristics of which are as follows: -pronon nuclear magnetic resonance spectrum (300 MHz; (CD3) 2SO d6; 6 in ppm; coupling constants J in Hz): 1.55 -2.10 (mt, 6H: CH2CH2 at 2 and CH2 at 3); 2.06 (s, 3H: SCH3); 2.30-2.65 and 2.75-2.30 (2 mts, respectively 4H and 2H: 2 CH2S and CH2 in 4); 3.30 and 3.35-3.50 (respectively dd, J = 14 and 8 and mt, 1H each: NCH 2 ); 3.43 (mt, 1H: CHN); 3.66 (mt, 1H: CH in 1); 4.37 (mt, 1H: CHCOO); 6.40-6.60 and 6.95 (2 mts, respectively 2H and 1H: H in 6 and H in 7 and H in 8); 7.95 (broad s, 3H: NH 3 +); 8.33 and 8.35 (2 d, J = 9, 1H: CONH). - elementary analysis: C19H29N3O3S2. 1.33 CF3CO2H
Calculé % : C = 46,2 ; H = 5,42 ; N = 7,46 ; S = 11, 38 ; F = 13,46 Trouvé : 45,8 ; 5,5 ; 7,47 ; 11,25 ; 13,2 L'activité inhibitrice de la farnésyltransférase et de la famésylation de la protéine Ras peut être mise en évidence dans le test suivant :Calculated%: C = 46.2; H = 5.42; N = 7.46; S = 11.38; F = 13.46 Found: 45.8; 5.5; 7.47; 11.25; 13.2 The inhibiting activity of farnesyltransferase and famesylation of the Ras protein can be demonstrated in the following test:
L'activité farnésyltransférase est déterminée par la quantité de (^H) farnesyl transférée à partir du (^H) farnésylpyrophosphate [(^H) FPP) sur la protéine p21 H-Ras. Le mélange réactionnel standard est composé, pour un volume final de 60 μl, de Tris-HCl 50mM, MgCl2 5mM, dithiotréitol 5 mM, octyl-β-D-glucopyranoside 0,2 , p21 H-ras 200 picomoles, (3H) FPP (à 61000 dpm/picomole) 4,5 picomoles.The farnesyltransferase activity is determined by the amount of (^ H) farnesyl transferred from (^ H) farnesylpyrophosphate [(^ H) FPP) to the p21 H-Ras protein. The standard reaction mixture is composed, for a final volume of 60 μl, of 50 mM Tris-HCl, 5 mM MgCl2, 5 mM dithiotreitol, octyl-β-D-glucopyranoside 0.2, p21 H-ras 200 picomoles, ( 3 H) FPP (at 61,000 dpm / picomole) 4.5 picomoles.
La réaction est initiée par addition d'environ 5 ng de farnésyltransférase humaine purifiée à partir de cultures de cellules THP1. Après incubation pendant 20 minutes à 37°C en plaque de microtitration contenant 96 trous de 1 cm3 par plaque (Titer Plate®, Beckman), la réaction est stoppée par addition de 0,4 cm3 de SDS à 0,1 % dans le méthanol à 0°C. Le mélange est ensuite additionné de 0,4 cm3 d'acide trichloroacétique (TCA) à 30 % dans le méthanol. Les plaques sont laissées pendant 1 heure dans la glace. Le contenu précipité est alors retenu sur membrane en fibre de verre Filtermat®, Pharmacia) avec l'unité de filtration (Combi Cell Harvester®, Skatron) et rincé avec de l'acide trichloroacétique à 6 % dans l'eau distillée. Les membranes sont séchées au four à micro-ondes puis imprégnées de scintillant par fusion sous air chaud de Meltilex® OPharmacia) et enfin comptées en cpm dans un compteur β-Plate® (LKB). Chaque essai est répété 3 fois.The reaction is initiated by the addition of approximately 5 ng of human farnesyltransferase purified from cultures of THP1 cells. After incubation for 20 minutes at 37 ° C. in a microtiter plate containing 96 holes of 1 cm 3 per plate (Titer Plate®, Beckman), the reaction is stopped by adding 0.4 cm 3 of 0.1% SDS in methanol. at 0 ° C. 0.4 cm 3 of trichloroacetic acid (TCA) 30% in methanol is then added to the mixture. The plates are left for 1 hour in ice. The precipitated content is then retained on a fiberglass membrane Filtermat®, Pharmacia) with the filtration unit (Combi Cell Harvester®, Skatron) and rinsed with 6% trichloroacetic acid in distilled water. The membranes are dried in the microwave oven then impregnated with scintillant by melting under hot air of Meltilex® OPharmacia) and finally counted in cpm in a β-Plate® counter (LKB). Each test is repeated 3 times.
L'unité d'activité est définie par 1 picomole de (-1H) FPP transférée sur p21 H-Ras en 20 minutes.The activity unit is defined by 1 picomole of (-1H) FPP transferred to p21 H-Ras in 20 minutes.
Les pourcentages d'inhibition sont obtenus par comparaison des essais avec et sans inhibiteur après déduction des blancs, les IC50 étant mesurées à partir des inhibitions obtenues avec 9 concentrations différentes en utilisant les logiciels Enzfitter® ou Grafit®. L'activité sur cellules peut être déterminée de la manière suivante :The inhibition percentages are obtained by comparison of the tests with and without inhibitor after deduction of the blanks, the IC50 being measured from the inhibitions obtained with 9 different concentrations using the Enzfitter® or Grafit® software. Cell activity can be determined as follows:
La lignée cellulaire est la lignée THAC (cellules CCL 39 transfectées par Ha-Ras activé) selon K. Seu en et coll., EMBO J., 2(1) 161-168 (1988). Les cellules sont ensemencées dans des boîtes de Pétri de 6 cm de diamètre contenant un milieu DMEM, sérum de veau foetal 5 %, G418 1 %. Après 24 heures de culture, le milieu de culture est changé (avec ou sans sérum) et le produit à étudier est ajouté en solution dans le diméthylformamide (DMF), en présence ou en absence de DTT (concentrations finales de 0,5 % en DMF et O.lmM en DTT). Après 24 heures de culture à 37°C, les cellules sont lysées dans 1 cm3 de tampon de lyse (Tris, HC1 20mM, Triton X114 1 Ψc, MgCl2 5 mM, DTT 7 mM, NaCl 150 mM, pH = 7,4). Les lysats sont clarifiés par centrifugation à 4.000 tours/minute pendant 10 minutes. L'extraction par le Triton XI 14 permet de séparer la protéine Ras farnésylée de la protéine Ras non famésylée [C. BORDIER, J. Biol. Chem., 25â (4), 1604-1607 (1981)]. La protéine Ras famésylée qui est plus hydro- phobe se trouve dans la phase détergente tandis que la protéine Ras non famésylée est dans la phase aqueuse. Les échantillons sont dénaturés par chauffage à 95°C dans du tampon de dénaturation pour électrophorèse et déposées sur un gel de polyacrylamide à 14 %. Lorsque le colorant atteint le bas du gel, les protéines du gel sont transférées sur une membrane PVDF. La protéine Ras est révélée par la technique de Western blot : la membrane est incubée avec un anticorps monoclonal spécifique anti-Ras (pan-Ras Ab3, Oncogène Science) puis avec de la protéine A marquée à 125j Après autoradiographie, les bandes sont identifiées, découpées et comptées dans un compteur γ. La radioactivité des bandes correspondant à Ras famésylée et à Ras non famésylée permet de déterminer le pourcentage d'inhibition de la famésylation de la protéine Ras. Les résultats obtenus sont rassemblés dans le tableau I. TABLEAU IThe cell line is the THAC line (CCL 39 cells transfected with activated Ha-Ras) according to K. Seu en et al., EMBO J., 2 (1) 161-168 (1988). The cells are seeded in 6 cm diameter petri dishes containing DMEM medium, 5% fetal calf serum, 1% G418. After 24 hours of culture, the culture medium is changed (with or without serum) and the product to be studied is added in solution in dimethylformamide (DMF), in the presence or in the absence of DTT (final concentrations of 0.5% in DMF and O.lmM in DTT). After 24 hours of culture at 37 ° C., the cells are lysed in 1 cm3 of lysis buffer (Tris, 20 mM HCl, Triton X114 1 Ψc, 5 mM MgCl2, 7 mM DTT, 150 mM NaCl, pH = 7.4) . The lysates are clarified by centrifugation at 4,000 rpm for 10 minutes. Extraction with Triton XI 14 makes it possible to separate the farnesylated Ras protein from the non-famesylated Ras protein [C. BORDIER, J. Biol. Chem., 25â (4), 1604-1607 (1981)]. The famesylated Ras protein, which is more hydrophobic, is found in the detergent phase while the non-famesylated Ras protein is found in the aqueous phase. The samples are denatured by heating to 95 ° C. in denaturation buffer for electrophoresis and deposited on a 14% polyacrylamide gel. When the dye reaches the bottom of the gel, the proteins in the gel are transferred to a PVDF membrane. The Ras protein is revealed by the Western blot technique: the membrane is incubated with a specific anti-Ras monoclonal antibody (pan-Ras Ab3, Oncogene Science) then with protein A labeled at 125 d. After autoradiography, the bands are identified, cut and counted in a γ counter. The radioactivity of the bands corresponding to famesylated Ras and to non-famesylated Ras makes it possible to determine the percentage inhibition of famesylation of the Ras protein. The results obtained are collated in Table I. TABLE I
PRODUIT Activité inhibitrice % d'inhibition sur IC50 nM cellules CTHAC)PRODUCT Inhibitory activity% inhibition on IC 50 nM CTHAC cells)
Exemple 1 15 > 10 (10 μM)Example 1 15> 10 (10 μM)
Exemple 2 405 50 (50 μM)Example 2 405 50 (50 μM)
Les nouveaux produits de formule générale (I) peuvent se présenter sous forme de sels non toxiques et pharmaceutiquement acceptables. Ces sels non toxiques comprennent les sels avec les acides minéraux (acides chlorhydrique, sulfurique, bromhydrique, phosphorique, nitrique) ou avec les acides organiques (acides acétique, propionique, succinique, maléique, hydroxymaléique, benzoïque, fumarique, méthanesulfonique, trifluoroacétique ou oxalique) ou avec les bases minérales (soude, potasse, lithine, chaux) ou organiques (aminés tertiaires comme la triéthylamine, la pipéridine, la benzylamine) selon la nature des symboles Rjet R du produit de formule générale (I).The new products of general formula (I) can be in the form of non-toxic and pharmaceutically acceptable salts. These non-toxic salts include the salts with mineral acids (hydrochloric, sulfuric, hydrobromic, phosphoric, nitric) or with organic acids (acetic, propionic, succinic, maleic, hydroxymaleic, benzoic, fumaric, methanesulfonic, trifluoroacetic or oxalic acids) or with mineral bases (soda, potash, lithine, lime) or organic bases (tertiary amines such as triethylamine, piperidine, benzylamine) depending on the nature of the Rjet R symbols of the product of general formula (I).
La présente invention concerne également les compositions pharmaceutiques qui contiennent au moins un produit de formule générale (I) en association avec un ou plusieurs diluants ou adjuvants pharmaceutiquement acceptables qu'ils soient inertes ou physiologiquement actifs.The present invention also relates to pharmaceutical compositions which contain at least one product of general formula (I) in combination with one or more diluents or pharmaceutically acceptable adjuvants whether inert or physiologically active.
Ces compositions peuvent être administrées par voie orale, parentérale ou rectale.These compositions can be administered orally, parenterally or rectally.
Les compositions pour administration orale comprennent des comprimés, des pilules, des poudres ou des granulés. Dans ces compositions le produit actif selon l'invention est mélangé à un ou plusieurs diluants inertes tels que saccharose, lactose ou amidon. Ces compositions peuvent comprendre des substances autres que les diluants, par exemple un lubrifiant tel que le stéarate de magnésium.The compositions for oral administration include tablets, pills, powders or granules. In these compositions, the active product according to the invention is mixed with one or more inert diluents such as sucrose, lactose or starch. These compositions can include substances other than diluents, for example a lubricant such as magnesium stearate.
Comme compositions liquides pour administration orale peuvent être utilisées des émulsions pharmaceutiquement acceptables, des solutions, des suspensions, des sirops, des élixirs contenant des diluants inertes tel que l'eau ou l'huile de paraffine. Ces compositions peuvent également comprendre des substances autres que les diluants, par exemple des produits mouillants, édulcorants ou aromatisants. Les compositions selon l'invention pour administration parentérale peuvent être des solutions stériles aqueuses ou non aqueuses, des suspensions ou des émulsions. Comme solvant ou véhicule, on peut employer le propylèneglycol, un polyéthylèneglycol, des huiles végétales, en particulier l'huile d'olive ou des esters organiques injectables par exemple l'oléate d'éthyle. Ces compositions peuvent également contenir des adjuvants, en particulier des agents mouillants, émulsifiants et dispersants. La stérilisation peut se faire de plusieurs façons, par exemple à l'aide d'un filtre bactériologique, en incorporant à la composition des agents stérilisants ou par chauffage. Elles peuvent être également préparées sous forme de compositions solides stériles qui peuvent être dissoutes au moment de l'emploi dans de l'eau stérile ou tout autre milieu stérile injectable.As liquid compositions for oral administration can be used pharmaceutically acceptable emulsions, solutions, suspensions, syrups, elixirs containing inert diluents such as water or paraffin oil. These compositions can also include substances other than diluents, for example wetting, sweetening or flavoring products. The compositions according to the invention for parenteral administration can be sterile aqueous or non-aqueous solutions, suspensions or emulsions. As solvent or vehicle, propylene glycol, a polyethylene glycol, vegetable oils, in particular olive oil or injectable organic esters, for example ethyl oleate, can be used. These compositions can also contain adjuvants, in particular wetting agents, emulsifiers and dispersants. Sterilization can be done in several ways, for example using a bacteriological filter, incorporating sterilizing agents into the composition or by heating. They can also be prepared in the form of sterile solid compositions which can be dissolved at the time of use in sterile water or any other sterile injectable medium.
Les compositions pour administration rectale sont des suppositoires qui peuvent contenir, outre le produit actif, des excipients tels que le beurre de cacao.The compositions for rectal administration are suppositories which may contain, in addition to the active product, excipients such as cocoa butter.
Les compositions selon l'invention sont particulièrement utiles en thérapeu- tique humaine dans le traitement de cancers d'origines diverses.The compositions according to the invention are particularly useful in human therapy in the treatment of cancers of various origins.
En thérapeutique humaine, les doses dépendent de l'effet recherché, de la durée du traitement et des facteurs propres au sujet à traiter.In human therapy, the doses depend on the desired effect, on the duration of the treatment and on the factors specific to the subject to be treated.
Généralement, les doses sont comprises, chez l'homme, entre 0,1 et 20 mg/kg par jour par voie intra-péritonéale. L'exemple suivant illustre une composition selon l'invention.Generally, the doses are, in humans, between 0.1 and 20 mg / kg per day intraperitoneally. The following example illustrates a composition according to the invention.
EXEMPLEEXAMPLE
On dissout 200 mg du produit obtenu à l'exemple 1 dans 100 cm3 de sérum physiologique. La solution obtenue est répartie aseptiquement en ampoules de 10 cm3. Les ampoules sont administrées en injection unique ou en perfusion. 200 mg of the product obtained in Example 1 are dissolved in 100 cm 3 of physiological saline. The solution obtained is aseptically distributed in 10 cm 3 ampoules. The ampoules are administered as a single injection or as an infusion.

Claims

REVENDICATIONS
1 - Nouveaux produits de formule générale :1 - New products of general formula:
X^Y. l dans laquelle : Ri représente un radical de formule générale Y-S-Aj- dans lequel Y représente un atome d'hydrogène, ou un reste d'aminoacide ou un reste d'acide gras ou un radical alkyle ou alkoxycarbonyle, et Aj représente un radical alcoylène droit ou ramifié contenant 1 à 4 atomes de carbone éventuellement substitué en α du groupement >C(Xj)(Yι) par un radical amino, alkylamino, alkanoylamino, alkoxycarbonylamino dont la partie alkyle ou alkanoyle droite ou ramifiée contient 1 à 6 atomes de carbone, Xj et Yj représentent chacun un atome d'hydrogène ou forment ensemble avec l'atome de carbone auquel ils sont liés un groupement >C=O,X ^ Y. l in which: Ri represents a radical of general formula YS-Aj- in which Y represents a hydrogen atom, or an amino acid residue or a fatty acid residue or an alkyl or alkoxycarbonyl radical, and Aj represents a radical straight or branched alkylene containing 1 to 4 carbon atoms optionally substituted at α in the group> C (Xj) (Yι) by an amino, alkylamino, alkanoylamino, alkoxycarbonylamino radical, the straight or branched alkyl or alkanoyl part of which contains 1 to 6 atoms carbon, Xj and Yj each represent a hydrogen atom or form together with the carbon atom to which they are linked a group> C = O,
R'i représente un atome d'hydrogène ou un radical alkyle droit ou ramifié contenant 1 à 6 atomes de carbone, X représente un atome d'oxygène ou de soufre,R'i represents a hydrogen atom or a straight or branched alkyl radical containing 1 to 6 carbon atoms, X represents an oxygen or sulfur atom,
R2 représente un radical alkyle, alkényle ou alkynyle droit ou ramifié contenant 1 à 6 atomes de carbone éventuellement substitué par un radical hydroxy, alkoxy contenant 1 à 4 atomes de carbone, mercapto, alkylthio contenant 1 à 4 atomes de carbone, alkylsulfinyle contenant 1 à 4 atomes de carbone ou alkylsulfonyle contenant 1 à 4 atomes de carbone, étant entendu que, lorsque R2 représente un radical alkyle substitué par un radical hydroxy, R2 peut former avec le radical carboxy en α une lactone,R2 represents an alkyl, alkenyl or straight or branched alkynyl radical containing 1 to 6 carbon atoms optionally substituted by a hydroxy radical, alkoxy containing 1 to 4 carbon atoms, mercapto, alkylthio containing 1 to 4 carbon atoms, alkylsulfinyl containing 1 to 4 carbon atoms or alkylsulfonyl containing 1 to 4 carbon atoms, it being understood that, when R2 represents an alkyl radical substituted by a hydroxy radical, R2 can form with the carboxy radical in α a lactone,
R'2 représente un atome d'hydrogène ou un radical alkyle droit ou ramifié contenant 1 à 6 atomes de carbone, et R représente un atome d'hydrogène ou un radical alcoyle contenant 1 à 6 atomes de carbone éventuellement substitué par un radical alcoxy contenant 1 à 4 atomes de carbone, alcoylthio contenant 1 à 4 atomes de carbone, alkylsulfinyle contenant 1 à 4 atomes de carbone, alkylsulfonyle contenant 1 à 4 atomes de carbone, phényle, phénoxy, phénylthio, phénylsulfinyl, phénylsulfonyl, alcoylamino contenant 1 à 4 atomes de carbone, dialcoylamino dont chaque partie alcoyle contient 1 à 4 atomes de carbone, ou un radical phényle éventuellement substitué par un ou plusieurs atomes ou radicaux choisis parmi les atomes d'halogène et les radicaux alcoyles, alcoyloxy, alcoylthio ou alcanoyle.R'2 represents a hydrogen atom or a straight or branched alkyl radical containing 1 to 6 carbon atoms, and R represents a hydrogen atom or an alkyl radical containing 1 to 6 carbon atoms optionally substituted by an alkoxy radical containing 1 to 4 carbon atoms, alkylthio containing 1 to 4 carbon atoms, alkylsulfinyl containing 1 to 4 carbon atoms, alkylsulfonyl containing 1 to 4 carbon atoms, phenyl, phenoxy, phenylthio, phenylsulfinyl, phenylsulfonyl, alkylamino containing 1 to 4 carbon atoms, dialkoylamino in which each alkyl part contains 1 to 4 carbon atoms, or a phenyl radical optionally substituted by one or more atoms or radicals chosen from halogen atoms and the alkyl, alkyloxy, alkylthio or alkanoyl radicals.
2 - Nouveaux produits selon la revendication 1 pour lesquels :2 - New products according to claim 1 for which:
Rj représente un radical de formule Y-S-Aj- dans lequel Y représente un atome d'hydrogène ou un reste lysine ou un reste d'acide gras contenant jusqu'à 20 atomes de carbone et Aj représente un radical éthylène ou propylène éventuellement substitué par un radical amino,Rj represents a radical of formula YS-Aj- in which Y represents a hydrogen atom or a lysine residue or a fatty acid residue containing up to 20 carbon atoms and Aj represents an ethylene or propylene radical optionally substituted by a amino radical,
Xj et Yj représentent chacun un atome d'hydrogène ou forment ensemble avec l'atome de carbone auquel ils sont liés un groupement >C=O,Xj and Yj each represent a hydrogen atom or form together with the carbon atom to which they are linked a group> C = O,
R'i représente un atome d'hydrogène ou un radical méthyle,R'i represents a hydrogen atom or a methyl radical,
X représente un atome d'oxygène, R2 représente un radical alkyle contenant 1 à 4 atomes de carbone éventuellement substitué par un radical hydroxy, méthoxy, mercapto, méthylthio, méthylsulfinyle ou méthylsulfonyle,X represents an oxygen atom, R2 represents an alkyl radical containing 1 to 4 carbon atoms optionally substituted by a hydroxy, methoxy, mercapto, methylthio, methylsulfinyl or methylsulfonyl radical,
R'2 représente un atome d'hydrogène ou un radical méthyle, etR'2 represents a hydrogen atom or a methyl radical, and
R représente un atome d'hydrogène ou un radical alcoyle contenant 1 à 4 atomes de carbone, éventuellement substitué par un radical alcoxy, ou un radical phényle.R represents a hydrogen atom or an alkyl radical containing 1 to 4 carbon atoms, optionally substituted by an alkoxy radical, or a phenyl radical.
3 - Nouveaux produits selon la revendication 1 pour lesquels :3 - New products according to claim 1 for which:
Rj représente un radical de formule Y-S-Aj- dans lequel Y représente un atome d'hydrogène et A représente un radical éthylène ou propylène éventuellement substitué par un radical amino, Xj et Yj représentent chacun un atome d'hydrogène ou forment ensemble avec l'atome de carbone auquel ils sont liés un groupement >C=O,Rj represents a radical of formula YS-Aj- in which Y represents a hydrogen atom and A represents an ethylene or propylene radical optionally substituted by an amino radical, Xj and Yj each represent a hydrogen atom or form together with the carbon atom to which they are linked a group> C = O,
R'i représente un atome d'hydrogène,R'i represents a hydrogen atom,
X représente un atome d'oxygène,X represents an oxygen atom,
R2 représente un radical méthyle, éthyle, propyle ou butyle éventuellement substitué par un radical hydroxy, méthoxy, mercapto ou méthylthio,R2 represents a methyl, ethyl, propyl or butyl radical optionally substituted by a hydroxy, methoxy, mercapto or methylthio radical,
R'2 représente un atome d'hydrogène, etR'2 represents a hydrogen atom, and
R représente un atome d'hydrogène ou un radical alcoyle contenant 1 à 4 atomes de carbone. 4 - Nouveaux produits selon la revendication 1 pour lesquels Rj représente un radical mercapto-2 éthyle ou amino- 1 mercapto-2 éthyle, X\ et Yj représentent chacun un atome d'hydrogène ou forment ensemble avec l'atome de carbone auquel ils sont liés un groupement >C=O, R'j représente un atome d'hydrogène, X représente un atome d'oxygène, R2 représente un radical n.butyle ou méthylthio-2 éthyle et R'2 représente un atome d'hydrogène, et R représente un atome d'hydrogène ou un radical méthyle.R represents a hydrogen atom or an alkyl radical containing 1 to 4 carbon atoms. 4 - New products according to claim 1 for which Rj represents a 2-mercapto-ethyl or amino-1-mercapto-2-ethyl radical, X \ and Yj each represent a hydrogen atom or form together with the carbon atom to which they are linked a group> C = O, R'j represents a hydrogen atom, X represents an oxygen atom, R2 represents an n.butyl or 2-methylthioethyl radical and R'2 represents a hydrogen atom, and R represents a hydrogen atom or a methyl radical.
5 - Composition pharmaceutique caractérisée en ce qu'elle contient une quantité suffisante d'un produit selon l'une des revendications 1 à 4 en association avec un ou plusieurs diluants ou adjuvants pharmaceutiquement acceptables inertes ou physiologiquement actifs. 5 - Pharmaceutical composition characterized in that it contains a sufficient amount of a product according to one of claims 1 to 4 in combination with one or more diluents or pharmaceutically acceptable adjuvants inert or physiologically active.
EP96901397A 1995-01-18 1996-01-16 Novel farnesyl transferase inhibitors, preparation thereof and pharmaceutical compositions containing same Ceased EP0804415A1 (en)

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