EP0799204A1 - 1,3,3-(trisubstituted)cyclohexane monomers and related compounds - Google Patents

Abstract

This invention relates to certain 1,3,3-(trisubstituted)cyclohexane monomers and related compounds which are useful in treating allergic and inflammatory diseases and for inhibiting the production of Tumor Necrosis Factor (TNF).

Description

l,3,3-(Trisubstituted)cyclohexane Monomers and Related

Compounds

Field of Invention

The present invention relates to novel l,3,3-(trisubstituted)cyclohexane monomers and related compounds, pharmaceutical compositions containing these compounds, and their use in treating allergic and inflammatory diseases and for inhibiting the production of Tumor Necrosis Factor (TNF).

Background of the Invention

Bronchial asthma is a complex, multifactorial disease characterized by reversible narrowing of the airway and hyperreactivity of the respiratory tract to external stimuli. Identification of novel therapeutic agents for asthma is made difficult by the fact that multiple mediators are responsible for the development of the disease. Thus, it seems unlikely that eliminating the effects of a single mediator will have a substantial effect on all three components of chronic asthma. An alternative to the "mediator approach" is to regulate the activity of the cells responsible for the pathophysiology of the disease.

One such way is by elevating levels of cAMP (adenosine cyclic 3',5'- monophosphate). Cyclic AMP has been shown to be a second messenger mediating the biologic responses to a wide range of hormones, neurotransmitters and drugs; [Krebs Endocrinology Proceedings of the 4th International Congress Excerpta Medica, 17-29, 1973]. When the appropriate agonist binds to specific cell surface receptors, adenylate cyclase is activated, which converts Mg⁺2-ATP to cAMP at an accelerated rate.

Cyclic AMP modulates the activity of most, if not all, of the cells that contribute to the pathophysiology of extrinsic (allergic) asthma. As such, an elevation of cAMP would produce beneficial effects including: 1) airway smooth muscle relaxation, 2) inhibition of mast cell mediator release, 3) suppression of neutrophil degranulation, 4) inhibition of basophil degranulation, and 5) inhibition of monocyte and macrophage activation. Hence, compounds that activate adenylate cyclase or inhibit phosphodiesterase should be effective in suppressing the inappropriate activation of airway smooth muscle and a wide variety of inflammatory cells. The principal cellular mechanism for the inactivation of cAMP is hydrolysis of the 3'-phosphodiester bond by one or more of a family of isozymes referred to as cyclic nucleotide phosphodiesterases (PDEs).

It has now been shown that a distinct cyclic nucleotide phosphodiesterase (PDE) isozyme, PDE IV, is responsible for cAMP breakdown in airway smooth muscle and inflammatory cells. [Torphy, "Phosphodiesterase Isozymes: Potential Targets for Novel Anti-asthmatic Agents" in New Drugs for Asthma, Barnes, ed. IBC Technical Services Ltd., 1989]. Research indicates that inhibition of this enzyme not only produces airway smooth muscle relaxation, but also suppresses degranulation of mast cells, basophils and neutrophils along with inhibiting the activation of monocytes and neutrophils. Moreover, the beneficial effects of PDE IV inhibitors are markedly potentiated when adenylate cyclase activity of target cells is elevated by appropriate hormones or autocoids, as would be the case in vivo. Thus PDE IV inhibitors would be effective in the asthmatic lung, where levels of prostaglandin E2 and prostacyclin (activators of adenylate cyclase) are elevated. Such compounds would offer a unique approach toward the pharmacotherapy of bronchial asthma and possess significant therapeutic advantages over agents currently on the market. The compounds of this invention also inhibit the production of Tumor Necrosis

Factor (TNF), a serum glycoprotein. Excessive or unregulated TNF production has been implicated in mediating or exacerbating a number of diseases including rheumatoid arthritis, rheumatoid spondylitis, os-eoarthri-is, gouty arthritis and other arthritic conditions; sepsis, septic shock, endotoxic shock, gram negative sepsis, toxic shock syndrome, adult respiratory distress syndrome, cerebral malaria, chronic pulmonary inflammatory disease, silicosis, pulmonary sarcoidosis, bone resorption diseases, reperfusion injury, graft vs. host reaction, allograft rejections, fever and myalgias due to infection, such as influenza, cachexia secondary to infection or malignancy, cachexia secondary to human acquired immune deficiency syndrome (AIDS), AIDS, ARC (AIDS related complex), keloid formation, scar tissue formation, Crohn's disease, ulcerative colitis, or pyresis, in addition to a number of autoimmune diseases, such as multiple sclerosis, autoimmune diabetes and systemic lupus erythematosis.

AIDS results from the infection of T lymphocytes with Human Immunodeficiency Virus (HIV). At least three types or strains of HIV have been identified, i.e., HIV-1, HIV-2 and HIV-3. As a consequence of HIV infection, T-cell- mediated immunity is impaired and infected individuals manifest severe opportunistic infections and/or unusual neoplasms. HIV entry into the T lymphocyte requires T lymphocyte activation. Viruses such as HIV-1 or HIV-2 infect T lymphocytes after T cell activation and such virus protein expression and/or replication is mediated or maintained by such T cell activation. Once an activated T lymphocyte is infected with HIV, the T lymphocyte must continue to be maintained in an activated state to permit HIV gene expression and/or HIV replication.

Cytokines, specifically TNF, are implicated in activated T-cell-mediated HIV protein expression and/or virus replication by playing a role in maintaining T lymphocyte activation. Therefore, interference with cytokine activity such as by inhibition of cytokine production, notably TNF, in an HTV-infected individual aids in limiting the maintenance of T cell activation, thereby reducing the progression of HIV infectivity to previously uninfected cells which results in a slowing or elimination of the progression of immune dysfunction caused by HIV infection. Monocytes, macrophages, and related cells, such as kupffer and glial cells, have also been implicated in maintenance of the HIV infection. These cells, like T cells, are targets for viral replication and the level of viral replication is dependent upon the activation state of the cells. [See Rosenberg et al. , The Immunopathogenesis of HIV Infection, Advances in Immunology, Vol. 57, 1989]. Monokines, such as TNF, have been shown to activate HIV replication in monocytes and/or macrophages [See Poli et al, Proc. Natl. Acad. Sci., 87:782-784, 1990], therefore, inhibition of monokine production or activity aids in limiting HIV progression as stated above for T cells.

TNF has also been implicated in various roles with other viral infections, such as the cytomegalovirus (CMV), influenza virus, adenovirus, and the herpes virus for similar reasons as those noted.

TNF is also associated with yeast and fungal infections. Specifically Candida albicans has been shown to induce TNF production in vitro in human monocytes and natural killer cells. [See Riipi et al, Infection and Immunity, 58(9):2750-54, 1990; and Jafari et al. Journal of Infectious Diseases, 164:389-95, 1991. See also Wasan et al, Antimicrobial Agents and Chemotherapy, 35,(10):2046-48, 1991 ; and Luke et al. Journal of Infectious Diseases, 162:21 1-214,1990].

The ability to control the adverse effects of TNF is furthered by the use of the compounds which inhibit TNF in mammals who are in need of such use. There remains a need for compounds which are useful in treating TNF-mediated disease states which are exacerbated or caused by the excessive and or unregulated production of TNF. In a first aspect, this invention relates to compounds of Formula (I):

wherein:

R] is selected from -(CR4R5)_nC(O)O(CR4R5)_mR6- -(CR4R5)nC(O)NR4(CR4R5)mR6, -(CR4R5)_nO(CR4R5)π_R6, or -(CR4R5)rR6 wherein the alkyl moieties is unsubstituted or substituted with one or more fluorines; m is 0 to 2; n is 0 to 4; r is 0 to 6;

R4 and R5 are independently selected from hydrogen or Ci-2 alkyl; R6 is hydrogen, methyl, hydroxyl, aryl, halo substituted aryl, aryloxyC] -3 alkyl, halo substituted aryloxyC 1.3 alkyl, indanyl, indenyl, C7-11 polycycloalkyl, tetrahydrofuranyl, furanyl, tetrahydropyranyl, pyranyl, tetrahydrothienyl, thienyl, tetrahydrothiopyranyl, thiopyranyl, C3_6 cycloalkyl, or a C4.6 cycloalkyl containing one or two unsaturated bonds, wherein the cycloalkyl or heterocyclic moiety is unsubstituted or substituted by 1 to 3 methyl groups, one ethyl group, or an hydroxyl group; provided that: a) when R6 is hydroxyl, then m is 2; or b) when R6 is hydroxyl, then r is 2 to 6; or c) when R6 is 2-tetrahydropyranyl, 2-tetrahydrothiopyranyl, 2-tetrahydrofuranyl, or 2-tetrahydrothienyl, then m is 1 or 2; or d) when R6 is 2-tetrahydropyranyl, 2-tetrahydrothiopyranyl, 2-tetrahydrofuranyl, or 2-tetrahydrothienyl, then r is 1 to 6; e) when n is 1 and m is 0, then R is other than H in

-(CR4R5)nO(CR4R5)mR6; X is YR2, halogen, fluorine, NR4R5, or formyl amine;

Y is O or S(O)_m-, m' is O, l, or 2;

X2 is O or NR8;

X3 is hydrogen or X; R2 is selected from the group consisting of -CH3 and -CH2CH3 optionally substituted by 1 or more fluorines;

R3 is COOR14, C(O)NR4Ri4 or R7; s is 0 to 4;

W is alkyl of 2 to 6 carbons, alkenyl of 2 to 6 carbon atoms or alkynyl of 2 to 6 carbon atoms;

Z is CR8R8OR.4- CR8R8OR15, CRδR8SRl4, CRsR8SRl5, CR8R8S(O)m'R7, CR8R8NR.θRl4, CR8R8NRlθS(O)2NRιoRl4, CR8R8NR_θS(O)2R7, CR8R8NR_θC(Y')Rl4, CR8R8NR.θQO)OR7, CR8R8NRlθC(Y')NRioRl4, CR8R8NR.θC(NCN)NRioRl4, CR8R8NR_oC(CR4NO2)NRioRl4, CR8R8NRlθC(NCN)SR9,

CR8R8NRl0C(CR4NO2)SR9, CR8RδC(O)ORi4, CR8 8C(Y')NRlθRl4, CR8R8C(NRio)NRioRl4, CRδRδCN, CRsR8(tetrazolyl), CRsRsGmidazolyl), CR8R8(imidazolidinyl), CRsR8(pyrazolyl), CR8R8(thiazolyl), CR8R8(thiazolidinyl), CR8R8(oxazolyl), CRsR8(oxazolidinyl), CRsR8(triazolyl), CRsR8(isoxazolyl), CR8R8(oxadiazolyl), CR8R8(thiadiazolyl), CR8R8(morpholinyl), CR8R8(piperidinyl), CR8R8(piperazinyl), CR8R8(pyrrolyl), CRsR8C(NOR8)Rl4, CR8RδC(NOR_4)R8, CR8R8NRlθC(NRιo)SR9, CR8R8NRiθC(NRιo)NRιoRl4, CR8R8NRlθC(O)C(O)NRioRl4, or CRsR8NRlθC(O)C(O)ORi4; X5 is H, R9, OR8, CN, C(O)R8, C(O)OR8, C(O)NRδR8, or NRδRδ; X is H, R9, OR8, CN, C(O)Rδ, C(O)OR8, C(O)NRδR8, or NRδR ;

Y' is O or S;

R7 is -(CR4R5)ςRi2 or C]_6 alkyl wherein the R12 or C\. alkyl group is unsubstituted or substituted one or more times by methyl or ethyl unsubstituted or substituted by 1-3 fluorines, -F, -Br, -Cl, -NO₂, -NRIQRI 1 , -C(O)R8, -CO2R8, -O(CH₂)_qR8, -CN, -C(O)NRi(>Rι 1, -O(CH₂)_qC(O)NRιoRl 1 , -O(CH₂)_qC(O)R9, -NRioC(O)NRιoRl 1 , -NRιoC(O)Rι 1, -NRι₀C(O)OR9, -NRιoC(O)Ri3, -C(NRiθ)NRlθRl l, -C(NCN)NRιoRl l , -C(NCN)SR9, -NRioC(NCN)SR9 , -NRioC(NCN)NRioRl 1, -NRιoS(O)2R9, -S(O)_m'R9, -NRιoC(O)C(O)NRιoRl 1 , - NR 1 oC(O)C(O)R 10, or R 13 ; q is 0, 1, or 2;

Rl2 is R13, C3-C7 cycloalkyl, or an unsubstituted or substituted aryl or heteroaryl group selected from the group consisting of (2-, 3- or 4-pyridyl), pyrimidyl, pyrazolyl, (1- or 2-imidazolyl), pyrrolyl, piperazinyl, piperidinyl, morpholinyl, furanyl, (2- or 3-thienyl), quinolinyl, naphthyl, and phenyl;

Rl2 is selected from R13, C3-7 cycloalkyl, (2-, 3- or 4-pyridyl), pyrimidyl, pyrazolyl, (1- or 2-imidazolyl), pyrrolyl, piperazinyl, piperidinyl, morpholinyl, furanyl, (2- or 3-thienyl), quinolinyl, naphthyl, or phenyl;

Rg is hydrogen or R9; R9 is C- _4 alkyl optionally substituted by one to three fluorines;

RlO is ORδ or Rn ;

Rl j is hydrogen, or C 1.4 alkyl optionally substituted by one to three fluorines; or when Rio and Rl 1 are as NRioRl 1 they may together with the nitrogen form a 5 to 7 membered ring comprised of carbon or carbon and at least one additional heteroatom selected from O, N, or S;

R 13 is a substituted or unsubstituted heteroaryl group selected from the group consisting of oxazolidinyl, oxazolyl, thiazolyl, pyrazolyl, triazolyl, tetrazolyl, imidazolyl, imidazolidinyl, thiazolidinyl, isoxazolyl, oxadiazolyl, and thiadiazolyl, and where R_{i 3} is substituted on R₁₂ or R_]3 the rings are connected through a carbon atom and each second R_{J 3} ring may be unsubstituted or substituted by one or two Cj-2 alkyl groups unsubstituted or substituted on the methyl with 1 to 3 fluoro atoms;

Rl4 is hydrogen or R7; or when R and R14 are as NRδRl4 they may together with the nitrogen form a 5 to 7 membered ring comprised of carbon or carbon and one or more additional heteroatoms selected from O, N, or S; R₁₅ is C(O)Ri4, C(O)NR4Ri4, S(O)2R7, or S(O)2NR4Rl4; provided that:

(f) R7 is not Cι_4 alkyl optionally substituted by one to three fluorines; or the pharmaceutically acceptable salts thereof. This invention also relates to the pharmaceutical compositions comprising a compound of Formula (I) and a pharmaceutically acceptable carrier or diluent. The invention also relates to a method of mediation or inhibition of the enzymatic activity (or catalytic activity) of PDE IV in mammals, including humans, which comprises administering to a mammal in need thereof an effective amount of a compound of Formula (I) as shown below.

The invention further provides a method for the treatment of allergic and inflammatory disease which comprises administering to a mammal, including humans, in need thereof, an effective amount of a compound of Formula (I). The invention also provides a method for the treatment of asthma which comprises administering to a mammal, including humans, in need thereof, an effective amount of a compound of Formula (I).

This invention also relates to a method of inhibiting TNF production in a mammal, including humans, which method comprises administering to a mammal in need of such treatment, an effective TNF inhibiting amount of a compound of Formula (I). This method may be used for the prophylactic treatment or prevention of certain TNF mediated disease states amenable thereto.

This invention also relates to a method of treating a human afflicted with a human immunodeficiency virus (HIV), which comprises administering to such human an effective TNF inhibiting amount of a compound of Formula (I).

Compounds of Formula (I) are also useful in the treatment of additional viral infections, where such viruses are sensitive to upregulation by TNF or will elicit TNF production in vivo.

In addition, compounds of Formula (I) are also useful in treating yeast and fungal infections, where such yeast and fungi are sensitive to upregulation by TNF or will elicit TNF production in vivo.

Detailed Description of the Invention

This invention also relates to a method of mediating or inhibiting the enzymatic activity (or catalytic activity) of PDE TV in a mammal in need thereof and to inhibiting the production of TNF in a mammal in need thereof, which comprises administering to said mammal an effective amount of a compound of Formula (I).

Phosphodiesterase IV inhibitors are useful in the treatment of a variety of allergic and inflammatory diseases including: asthma, chronic bronchitis, atopic dermatitis, urticaria, allergic rhinitis, allergic conjunctivitis, vernal conjunctivitis, eosinophihc granuloma, psoriasis, rheumatoid arthritis, septic shock, ulcerative colitis, Crohn's disease, reperfusion injury of the myocardium and brain, chronic glomerulonephritis, endotoxic shock and adult respiratory distress syndrome. In addition, PDE IV inhibitors are useful in the treatment of diabetes insipidus and central nervous system disorders such as depression and multi-infarct dementia.

The viruses contemplated for treatment herein are those that produce TNF as a result of infection, or those which are sensitive to inhibition, such as by decreased replication, directly or indirectly, by the TNF inhibitors of Formula (I). Such viruses include, but are not limited to HIV-1, HIV-2 and HIV-3, cytomegalovirus (CMV), influenza, adenovirus and the Herpes group of viruses, such as, but not limited to, Herpes zoster and Herpes simplex.

This invention more specifically relates to a method of treating a mammal, afflicted with a human immunodeficiency virus (HIV), which comprises administering to such mammal an effective TNF inhibiting amount of a compound of Formula (I).

The compounds of this invention may also be used in association with the veterinary treatment of animals, other than in humans, in need of inhibition of TNF production. TNF mediated diseases for treatment, therapeutically or prophylactically, in animals include disease states such as those noted above, but in particular viral infections. Examples of such viruses include, but are not limited to feline immunodeficiency virus (FIV) or other retroviral infection such as equine infectious anemia virus, caprine arthritis virus, visna virus, maedi virus and other lentiviruses. The compounds of this invention are also useful in treating yeast and fungal infections, where such yeast and fungi are sensitive to upregulation by TNF or will elicit TNF production in vivo. A preferred disease state for treatment is fungal meningitis. Additionally, the compounds of Formula (I) may be administered in conjunction with other drugs of choice for systemic yeast and fungal infections. Drugs of choice for fungal infections, include but are not limited to the class of compounds called the polymixins, such as Polymycin B, the class of compounds called the imidazoles, such as clotrimazole, econazole, miconazole, and ketoconazole; the class of compounds called the triazoles, such as fluconazole, and itranazole, and the class of compound called the Amphotericins, in particular Amphotericin B and liposomal Amphotericin B.

The compounds of Formula (I) may also be used for inhibiting and/or reducing the toxicity of an anti-fungal, anti-bacterial or anti-viral agent by administering an effective amount of a compound of Formula (I) to a mammal in need of such treatment. Preferably, a compound of Formula (I) is administered for inhibiting or reducing the toxicity of the Amphotericin class of compounds, in particular Amphotericin B.

The term "Cι_₃ alkyl", "C1.4 alkyl", "Cγ.^ alkyl" or "alkyl" groups as used herein is meant to include both straight or branched chain radicals of 1 to 10, unless the chain length is limited thereto, including, but not limited to methyl, ethyl, n-propyl, isopropyl. n-butyl, sec-butyl, isobutyl, rt-butyl, and the like. "Alkenyl" means both straight or branched chain radicals of 1 to 6 carbon lengths, unless the chain length is limited thereto, including but not limited to vinyl, 1- propenyl, 2-propenyl, 2-propynyl, or 3-methyl-2-propenyl.

The term "cycloalkyl" or "cycloalkyl alkyl" means groups of 3-7 carbon atoms, such as cyclopropyl, cyclopropylmethyl, cyclopentyl, or cyclohexyl.

"Aryl" or "aralkyl", unless specified otherwise, means an aromatic ring or ring system of 6-10 carbon atoms, such as phenyl, benzyl, phenethyl, or naphthyl. Preferably the aryl is monocyclic, i.e, phenyl. The alkyl chain is meant to include both straight or branched chain radicals of 1 to 4 carbon atoms. "Heteroaryl" means an aromatic ring system containing one or more heteroatoms, such as imidazolyl, triazolyl, oxazolyl, pyridyl, pyrimidyl, pyrazolyl, pyrrolyl, furanyl, or thienyl.

"Halo" means all halogens, i.e., chloro, fluoro, bromo, or iodo.

"Inhibiting the production of IL-1" or "inhibiting the production of TNF" means: a) a decrease of excessive in vivo IL-1 or TNF levels, respectively, in a human to normal levels or below normal levels by inhibition of the in vivo release of IL-1 by all cells, including but not limited to monocytes or macrophages; b) a down regulation, at the translational or transcriptional level, of excessive in vivo IL-1 or TNF levels, respectively, in a human to normal levels or below normal levels; or c) a down regulation, by inhibition of the direct synthesis of IL- 1 or TNF levels as a postranslational event.

The phrase "TNF mediated disease or disease states" means any and all disease states in which TNF plays a role, either by production of TNF itself, or by TNF causing another cytokine to be released, such as but not limited to IL-1 or IL-6. A disease state in which IL-1, for instance is a major component, and whose production or action, is exacerbated or secreted in response to TNF, would therefore be considered a disease state mediated by TNF. As TNF-β (also known as lymphotoxin) has close structural homology with TNF-α (also known as cachectin), and since each induces similar biologic responses and binds to the same cellular receptor, both TNF-α and TNF-β are inhibited by the compounds of the present invention and thus are herein referred to collectively as "TNF" unless specifically delineated otherwise. Preferably TNF-α is inhibited. "Cytokine" means any secreted polypeptide that affects the functions of cells, and is a molecule which modulates interactions between cells in immune, inflammatory, or hematopoietic responses. A cytokine includes, but is not limited to, monokines and lymphokines regardless of which cells produce them. The cytokine inhibited by the present invention for use in the treatment of a HIV-infected human must be a cytokine which is implicated in (a) the initiation and/or maintenance of T cell activation and/or activated T cell-mediated HIV gene expression and/or replication, and/or (b) any cytokine-mediated disease associated problem such as cachexia or muscle degeneration. Preferrably, this cytokine is TNF-α. The preferred compounds for this invention are as follows: When Ri is an alkyl substituted by 1 or more halogens, the halogens are preferably fluorine and chlorine, more preferably a Ci-4 alkyl substituted by 1 or more fluorines. The preferred halo-substituted alkyl chain length is one or two carbons, and most preferred are the moieties -CF3, -CH2F, -CHF2, -CF2CHF2, -CH2CF3, and - CH2CHF2. Preferred R- substitutents are CH2-cyclopropyl, CH2-C5-6 cycloalkyl, C4-6 cycloalkyl with or without an OH group, C7-1 1 polycycloalkyl, (3- or 4- cyclopentenyl), phenyl, tetrahydrofuran-3-yl, benzyl or Ci-2 alkyl optionally substituted by 1 or more fluorines, -(CH2)l-3C(O)O(CH2)0-2CH3, -(CH2)l-3θ(CH2)0-2CH3, and -(CH2)2-4OH.

When the R- term contains the moiety (CR4R5), the R4 and R5 terms are independently hydrogen or alkyl. This allows for branching of the individual methylene units as (CR4R5)_n or (CR4R5)m; each repeating methylene unit is independent of the other, e.g., (CR4R5)_n wherein n is 2 can be -CH2CH(-CH3)-, for instance. The individual hydrogen atoms of the repeating methylene unit or the branching hydrocarbon can optionally be substituted by fluorine independent of each other to yield, for instance, the preferred R] substitutions, as noted above.

When R] is a C7-1 1 polycycloalkyl, examples are bicyclo[2.2.1]-heptyl, bicyclo[2.2.2]octyl, bicyclo[3.2.1]octyl, tricyclo[5.2.1.0²*⁶]decyl, etc. additional examples of which are described in Saccamano et al, WO 87/06576, published 5 November 1987.

W is preferably alkyl, alkenyl or alkynyl of 2 to 4 carbon atoms, and where it is alkenyl or alkynyl, that one or two double or triple bonds be present.

Z is preferably CRδRδORi4, CRδRδORis, CR8R8SR14, CR R SRlS, CR8RδS(O)m-R7, CRδRδNRlθRl4, CRδRδNS(O)2NRιoRl4, CRδR NS(O)2R7, CR8R8NRlθC(O)Ri4, CRδRδNRlθC(O)OR7, CRδRδNR iθC(O)NRιoR 14, CRδRδNR l oC(NCN)NR 1 oR 14. CR RδNR 1 oC(CR4NO2)NR 1 oR 14, CRδRδNRlθC(NCN)SR9, CRδRδNR 10C(CR4NO2)SR9, CRδRδC(O)ORi4, CRδRδC(O)NRioRl4, CRδRδC(NRio)NRιoRl4, CRδRδCN, CRδRδ(oxadiazolyl), CRδRδ(thiadiazolyl), CRδRδC(NORδ)Rl4, CRδRδC(NORi4)Rδ, CRgRδNR 1 oC(NR 1 o)SR9, CRδR NR l θC(NR 1 o)NR 1 oR 14,

CRδRδNR ιoC(O)C(O)NRιoR 14, or CRδRδNR ioC(O)C(O)OR 14; most preferred are those compounds wherein the R group of Z is H and the R14 group of Z is R4. X₄ is preferably H, OH, OCH3, CN, C(O)Rδ, C(O)OH, C(O)OCH3, C(O)NH2, CON(CH3)2, NH2, or N(CH3)2- The most preferred X4 groups are H, OH, CN, C(O)OH, C(O)NH2 or NH2.

Preferred X groups are those wherein X is YR2 and Y is oxygen. The preferred X2 group is oxygen. The preferred X3 is hydrogen. Preferred R2 groups, where applicable, are C 1-2 alkyl unsubstituted or substituted by 1 or more halogens. The halogen atoms are preferably fluorine and chlorine, more preferably fluorine. More preferred R2 groups are those wherein R2 is methyl, or the fluoro-substituted alkyls, specifically a Ci-2 alkyl, such as a -CF3, -CHF2, or -CH2CHF2 moiety. Most preferred are the -CHF2 and -CH3 moieties.

Preferred R7 moieties include unsubstituted or substituted -(CH2)0-2(2-, 3- or 4-pyridyl), (CH2)l-2(2-imidazolyl), (CH2)2(4-moφholinyl), (CH2)2(4-piperazinyl), (CH2) 1-2(2 -thienyl), (CH2)l-2(4-thiazolyl), unsubstituted or substituted pyrimidinyl, and substituted or unsubstituted (CH2)0-2phenyl. Preferred rings when Rio and Rl l in the moiety -NRioRl 1 together with the nitrogen to which they are attached form a 5 to 7 membered ring containing at least one additional heteroatom selected from O, N or S include, but are not limited to 1- imidazolyl, 2-(Rδ)- 1-imidazolyl, 1-pyrazolyl, 3-(Rδ)- 1-pyrazolyl, 1-triazolyl, 2- triazolyl, 5-(Rδ)- 1-triazolyl, 5-(Rδ)-2-triazolyl, 5-(R8)- 1-tetrazolyl, 5-(Rδ)-2-tetrazolyl, 1-tetrazolyl, 2-tetrazloyl, moφholinyl, piperazinyl, 4-(Rs)- 1 -piperazinyl, or pyrrolyl ring.

Preferred rings when Rio and R 14 in the moiety -NR10R14 together with the nitrogen to which they are attached form a 5 to 7 membered ring containing at least one additional heteroatom selected from O, N or S include, but are not limited to 1- imidazolyl, 1-pyrazolyl, 1-triazolyl, 2-triazolyl, 1-tetrazolyl, 2-tetrazolyl, moφholinyl, piperazinyl, and pyrrolyl. The respective rings may be additionally substituted, where applicable, on an available nitrogen or carbon by the moiety R7 as described herein for Formula (I). Illustrations of such carbon substitutions includes, but are not limited to, 2-(R7)- 1-imidazolyl, 4-(R7)- 1-imidazolyl, 5-(R7)- 1-imidazolyl, 3-(R7)- 1-pyrazolyl, 4-(R7)- 1-pyrazolyl, 5-(R7)- 1-pyrazolyl, 4-(R7)-2-triazolyl, 5-(R7)-2-triazolyl, 4-(R7)- 1-triazolyl, 5 -(R7)- 1-triazolyl, 5-(R7)- 1-tetrazolyl, and 5-(R7)-2-tetrazolyl. Applicable nitrogen substitution by R7 includes, but is not limited to, l-(R7)-2-tetrazolyl, 2-(R7)- 1-tetrazolyl, 4-(R7)-l-piperazinyl. Where applicable, the ring can be substituted one or more times by R7. Preferred groups for NR 1 oR 14 which contain a heterocyclic ring are 5-(R 14)- 1 - tetrazolyl, 2-(R 14)- 1-imidazolyl, 5-(Ri4)-2-tetrazolyl, or 4-(R--*.)-l-piperazinyl.

Preferred rings for R13 include (2-, 4- or 5-imidazolyl), (3-, 4- or 5-pyrazolyl), (4- or 5-triazolyl[ 1,2,3]), (3- or 5-triazolyl[ 1,2,4]), (5-tetrazolyl), (2-, 4- or 5-oxazolyl), (3-, 4- or 5-isoxazolyl), (3- or 5-oxadiazolyl[l,2,4]), (2-oxadiazolyl[ 1,3,4]), (2-thiadiazolyl[ 1,3,4]), (2-, 4-, or 5-thiazolyl), (2-, 4-, or 5-oxazolidinyl), (2-, 4-, or 5-thiazolidinyl), or (2-, 4-, or 5-imidazolidinyl).

When the R7 group is optionally substituted by a heterocyclic ring such as imidazolyl, pyrazolyl, triazolyl, tetrazolyl, or thiazolyl, the heterocyclic ring itself may be optionally substituted by Rδ either on an available nitrogen or carbon atom, such as l-(Rδ)-2-imidazolyl, 1 -(Rδ)-4-imidazolyl, l-(Rδ)-5-imidazolyl, l-(Rδ)-3-pyrazolyl, l-(Rδ)-4-pyrazolyl, l-(Rδ)-5-pyrazolyl, 1 -(Rδ)-4-triazolyl, or l-(Rδ)-5-triazolyl. Where applicable, the ring may be substituted one or more times by R&.

Preferred are those compounds of Formula (I) wherein R is -CH2- cyclopropyl, -CH2-C5-6 cycloalkyl, -C4-6 cycloalkyl unsubstituted or substituted by OH, tetrahydrofuran-3-yl, (3- or 4-cyclopentenyl), benzyl or -Ci-2 alkyl unsubstituted or substituted by 1 or more fluorines, and -(CH2)2-4 OH; R2 is methyl or fluoro- substituted alkyl, W is ethynyl or 1,3-butadiynyl; R is R₇ where R is an unsubstituted or substituted aryl or heteroaryl ring, X is YR2, and Z is CRδRδORi5, or CRδRδNRlθRl4-

Most preferred are those compounds wherein Rj is -CH2-cyclopropyl, cyclopentyl, 3-hydroxycyclopentyl, methyl or CF2H; X is YR2; Y is oxygen; X2 is oxygen; X3 is hydrogen; and R2 is CF2H or methyl, W is ethynyl or 1,3-butadiynyl, and R3 is a substituted or unsubstituted pyrimidinyl ring. The exemplified compounds are: c«-[3-(3-cyclopentyloxy-4-methoxyphenyl)-3-(4-pyridylethynyl)-l- (hydroxymethyl)cyclohexane], and cis - [3 -(3 -cyclopentyloxy-4-methoxypheny 1 )-3-(4-pyridy lethyny 1 )- 1 - (aminomethyl)cyclohexane] . All of the compounds of Formula (I) are useful in the method of inhibiting the production of TNF, preferably by macrophages, monocytes or macrophages and monocytes, in a mammal, including humans, in need thereof. All of the compounds of Formula (I) are useful in the method of inhibiting or mediating the enzymatic or catalytic activity of PDE IV and in treatment of disease states mediated thereby. Pharmaceutically acceptable salts of the instant compounds, where they can be prepared, are also intended to be covered by this invention. These salts will be ones which are acceptable in their application to a pharmaceutical use. By that it is meant that the salt will retain the biological activity of the parent compound and the salt will not have untoward or deleterious effects in its application and use in treating diseases.

Pharmaceutically acceptable salts are prepared in a standard manner. The parent compound, dissolved in a suitable solvent, is treated with an excess of an organic or inorganic acid, in the case of acid addition salts of a base, or an excess of organic or inorganic base where the molecule contains a COOH for example.

Pharmaceutical compositions of the present invention comprise a pharmaceutical carrier or diluent and some amount of a compound of the formula (I). The compound may be present in an amount to effect a physiological response, or it may be present in a lesser amount such that the user will need to take two or more units of the composition to effect the treatment intended. These compositions may be made up as a solid, liquid or in a gaseous form. Or one of these three forms may be transformed to another at the time of being administered such as when a solid is delivered by aerosol means, or when a liquid is delivered as a spray or aerosol.

The nature of the composition and the pharmaceutical carrier or diluent will, of course, depend upon the intended route of administration, for example parenterally, topically, orally or by inhalation.

For topical administration the pharmaceutical composition will be in the form of a cream, ointment, liniment, lotion, pastes, aerosols, and drops suitable for administration to the skin, eye, ear, or nose.

For parenteral administration the pharmaceutical composition will be in the form of a sterile injectable liquid such as an ampule or an aqueous or non-aqueous liquid suspension. For oral administration the pharmaceutical composition will be in the form of a tablet, capsule, powder, pellet, atroche, lozenge, syrup, liquid, or emulsion.

When the pharmaceutical composition is employed in the form of a solution or suspension, examples of appropriate pharmaceutical carriers or diluents include: for aqueous systems, water; for non-aqueous systems, ethanol, glycerin, propylene glycol, corn oil, cottonseed oil, peanut oil, sesame oil, liquid parafms and mixtures thereof with water; for solid systems, lactose, kaolin and mannitol; and for aerosol systems, dichlorodifluoromethane, chlorotrifluoroethane and compressed carbon dioxide. Also, in addition to the pharmaceutical carrier or diluent, the instant compositions may include other ingredients such as stabilizers, antioxidants, preservatives, lubricants, suspending agents, viscosity modifiers and the like, provided that the additional ingredients do not have a detrimental effect on the therapeutic action of the instant compositions.

The pharmaceutical preparations thus described are made following the conventional techniques of the pharmaceutical chemist as appropriate to the desired end product.

In these compositions, the amount of carrier or diluent will vary but preferably will be the major proportion of a suspension or solution of the active ingredient. When the diluent is a solid it may be present in lesser, equal or greater amounts than the solid active ingredient. Usually a compound of formula I is administered to a subject in a composition comprising a nontoxic amount sufficient to produce an inhibition of the symptoms of a disease in which leukotrienes are a factor. Topical formulations will contain between about 0.01 to 5.0% by weight of the active ingredient and will be applied as required as a preventative or curative agent to the affected area. When employed as an oral, or other ingested or injected regimen, the dosage of the composition is selected from the range of from 50 mg to 1000 mg of active ingredient for each administration. For convenience, equal doses will be administered 1 to 5 times daily with the daily dosage regimen being selected from about 50 mg to about 5000 mg. No unacceptable toxicological effects are expected when these compounds are administered in accordance with the present invention.

The following examples are set forth to illustrate the invention. These examples are not intended to limit the scope of the invention in any manner or fashion. Reference is made to the claims for what is reserved to the inventor hereunder. Methods Of Preparation

Synthetic Scheme(s) With Textual Description

Compounds of Formula (I) may be prepared by the processes disclosed herein which comprise reacting a terminal acetylene, wherein Z and X4 represent Z and X4 as defined in relation to Formula (I) or a group convertible to Z and X4, as, e.g., compound 1-Scheme 1. with an appropriate halide, R3X, wherein R3 represents R3 as defined in relation to Formula (I) or a group convertible to R3, in the presence of a suitable catalyst, such as a copper (I) halide and a bivalent or zerovalent palladium compound in the presence of, e.g., triphenylphosphine, in a suitable solvent, such as an amine, as in the procedure of Brandsma et al. (Syn. Comm., 1990, 20, lδδ9), provides a compound of the Formula 2-Scheme 1. Compounds of the Formula 1-Scheme 1 may be prepared by procedures analogous to those described in copending U.S.patent applications 0δ/131054 filed 1 October 1993 and its progeny PCT/US94/10δl6 filed 23 September 1994.

Scheme 1

-L

a) Pd(PPh 3)₄, PPh 3, Cul, R ₃ X, pipβridine

Alternatively, compounds of the Formula (I), wherein Z, X4 and R3 represent Z, X4 and R3 as defined in relation to Formula (I) or a group convertible to Z, X4 or R3, may be prepared from the corresponding ketones as, e.g., compound 1-Scheme 2. by the synthetic procedures described in copending U.S. patent applications 08/131054 filed 1 October 1993 and its progeny PCT/US94/10816 filed 23 September 1994; syntheses of such ketone starting materials are described in co-pending U.S. applications 08/130215 filed 01 October 1993 and PCT/US94/10815 filed 23 September 1994 or co-pending USSN 08/130213 and PCT application PCT/US94/10767 filed on 01 October 1993 and 23 September 1994 respectively.

Scheme 2

Alternatively, oxidative carbonylation of a terminal acetylene as, e.g. , compound 1-Scheme 3. using an appropriate metal salt, such as a copper salt with a catalytic amount of a palladium salt, in the presence of a suitable base as an acid trap, such as sodium acetate, in a suitable alcohol, such as methanol, as in the method of Tsuji et αl. (Tet. Lett., 1980, 21, 849), then provides the compound of the Formula ___. Scheme 3; such compounds may then be converted to other compounds of the Formula (I) by manipulation of the ketone as described above and by independent manipulation of the carboxylic ester moiety using standard transesterification or amidation conditions. Syntheses of such ketone starting materials are described in co- pending U.S. applications 08/130215 filed 01 October 1993 and PCT/US94/10815 filed 23 September 1994.

Scheme 3

COOCH ₃

a) PdCI ₂. CuCI ₂, NaO ₂CCH ₃ , CO, CH ₃OH; as inScheme 2

Likewise, oxidative carbonylation of a terminal acetylene as, e.g., compound _1_. Scheme 4. wherein Z and X4 represents Z and X4 as defined in relation to Formula (I) or a group convertible to Z or X4, using an appropriate metal salt, such as a copper salt with a catalytic amount of a palladium salt, in the presence of a suitable base as an acid trap, such as sodium acetate, in a suitable alcohol, such as methanol, as in the method of Tsuji et al. (Tet. Lett., 1980, 21, 849), then provides the compound of the Formula 2-Scheme 4: such compounds may then be converted to other compounds of the Formula (I) by manipulation of the carboxylic ester moiety using standard transesterification or amidation conditions.

Scheme 4

J_

a) PdCI ₂ , CuCI ₂, Na0 ₂CCH ₃ , CO, CH ₃OH Preparation of the remaining compounds of the Formula (I) may be accomplished by procedures analogous to those described above and in the Examples, infra.

It will be recognized that some compounds of the Formula (I) may exist in distinct diastereomeric forms possessing distinct physical and biological properties; such isomers may be separated by standard chromatographic methods.

Experimental Examples

Example 1 Preparation of cM-r3-(3-cyclopentyloxy-4-methoxyphenyl)-3-(4-pyridylethynyl)-l - (hydτoxymethyl)cyclohexane]

A suspension of cis-[3-(3-cyclopentyloxy-4-methoxyphenyl)-3-(4- pyridylethynyl)cyclohexane-l -carboxylic acid] (0.22 g, 0.52 mmol, prepared as described in a copending U.S. application filed on even date herewith and identified as P50296) in tetrahydrofuran (2.0 mL) with methanol (0.025 mL) and lithium borohydride (0.02 g, 0.78 mmol) is stirred overnight at room temperature under an argon atmosphere. The reaction mixture is partitioned between methylene chloride and acidic water, is extracted three times, is dried (magnesium sulfate) and is evaporated. Purification by flash column chromatography provides the product.

Example 2

Preparation of c.s-[3-(3-cyclopentyloxy-4-methoxyphenyl)-3-(4-pyridylethynyl)- 1 -

(aminomethyl)cyclohexane] A solution of c.-r-[3-(3-cyclopentyloxy-4-methoxyphenyl)-3-(4-pyridylethynyl)- l-(hydroxymethyl)cyclohexane] (0.067 g, 0.16 mmol) in tetrahydrofuran (2 mL) under an argon atmosphere is treated with triphenylphosphine (0.04 g, 0.16 mmol), phthalimide (0.02 g, 0.16 mmol) and then diethylazodicarboxylate (0.03 mL, 0.16 mmol) is added dropwise. The reaction flask is covered with foil and the mixture is stirred at room temperature for 30 h. The solvent is evaporated and the residue is purified by flash column chromatography, to provide the productphthalimide, which is dissolved in ethanol (0.5 mL) under an argon atmosphere and is stirred with hydrazine hydrate (0.08 mL, 0.15 mmol) for 3 days. The precipitate is removed by filtration, the filtrate is applied to a silica column and the product is eluted. UTILITY EXAMPLES

EXAMPLE A Inhibitory effect of compounds of Formula (I) on in vitro TNF production by human monocytes The inhibitory effect of compounds of Formula (I) on in vitro TNF production by human monocytes may be determined by the protocol as described in Badger et al,

EPO published Application 0411 754 A2, February 6, 1991, and in Hanna, WO

90/15534, December 27, 1990.

EXAMPLE B Two models of endotoxic shock have been utilized to determine in vivo TNF activity for the compounds of Formula (I). The protocol used in these models is described in Badger et al, EPO published Application 0411 754 A2, February 6, 1991, and in Hanna, WO 90/15534, December 27, 1990.

The compound of Example 1 herein demonstrated a positive in vivo response in reducing serum levels of TNF induced by the injection of endotoxin.

EXAMPLE C Isolation of PDE Isozymes

The phosphodiesterase inhibitory activity and selectivity of the compounds of Formula (I) can be determined using a battery of five distinct PDE isozymes. The tissues used as sources of the different isozymes are as follows: 1) PDE lb, porcine aorta; 2) PDE Ic, guinea-pig heart; 3) PDE III, guinea-pig heart; 4) PDE IV, human monocyte; and 5) PDE V (also called "la"), canine trachealis. PDEs la, lb, Ic and III are partially purified using standard chromatographic techniques [Toφhy and Cieslinski, Mol. Pharmacol., 37:206-214, 1990]. PDE rv is purified to kinetic homogeneity by the sequential use of anion-exchange followed by heparin-Sepharose chromatography [Toφhy et a , J. Biol. Chem., 267:1798-1804, 1992].

Phosphodiesterase activity is assayed as described in the protocol of Toφhy and

Cieslinski, Mol. Pharmacol., 37:206-214, 1990. Positive ICso's in the nanomolar to μM range for compounds of the workings examples described herein for Formula (I) have been demonstrated.

Claims

What is claimed is:

1. A compound of Formula (I)

wherein:

Rl is selected from -(CR4R5)_nC(O)O(CR4R5)mR6,

-(CR4R5)nC(O)NR4(CR4R5)mR6, -(CR4R5)nO(CR4R5)mR6, or -(CR4R5)rR6 wherein the alkyl moieties is unsubstituted or substituted with one or more fluorines; m is 0 to 2; n is 0 to 4; r is 0 to 6;

R4 and R5 are independently selected from hydrogen or Ci-2 alkyl; R6 is hydrogen, methyl, hydroxyl, aryl, halo substituted aryl, aryloxyC 1.3 alkyl, halo substituted aryloxyC 1.3 alkyl, indanyl, indenyl, C7-11 polycycloalkyl, tetrahydrofuranyl, furanyl, tetrahydropyranyl, pyranyl, tetrahydrothienyl, thienyl, tetrahydrothiopyranyl, thiopyranyl, C3.6 cycloalkyl, or a C4-6 cycloalkyl containing one or two unsaturated bonds, wherein the cycloalkyl or heterocyclic moiety is unsubstituted or substituted by 1 to 3 methyl groups, one ethyl group, or an hydroxyl group; provided that: a) when R6 is hydroxyl, then m is 2; or b) when R6 is hydroxyl, then r is 2 to 6; or c) when R6 is 2-tetrahydropyranyl, 2-tetrahydrothiopyranyl, 2-tetrahydrofuranyl, or 2-tetrahydrothienyl, then m is 1 or 2; or d) when R6 is 2-tetrahydropyranyl, 2-tetrahydrothiopyranyl,

2-tetrahydrofuranyl, or 2-tetrahydrothienyl, then r is 1 to 6; e) when n is 1 and m is 0, then R6 is other than H in

-(CR4R5)nO(CR4R5)mR6;

X is YR2, halogen, fluorine, NR4R5, or formyl amine; Y is O or S(O)πϊ; m' is O, l, or 2; X2 is O or NR8; X3 is hydrogen or X; R2 is selected from the group consisting of -CH3 and -CH2CH3 optionally substituted by 1 or more fluorines;

R3 is COOR14, C(O)NR4Rl4 or R7; s is 0 to 4; W is alkyl of 2 to 6 carbons, alkenyl of 2 to 6 carbon atoms or alkynyl of 2 to 6 carbon atoms;

Z is CRδRδORi4, CRsRδORis, CRδRδSRl4, CRδRδSRl5, CRδRδS(O)m'R7, CRδR NRlθRl4, CRδR NR iθS(O)2NRlθR 14, CRδRδNRiθS(O)2R7, CRδRδNR ioC(Y^*)R 14, CRδRδNR lθC(O)OR7, CRδRδNRlθC(Y')NRioRl4, CRδRδ R lθC(NCN)NRιoR 14, CRδRδNRlθC(CR4Nθ2)NRiθRl4, CRδRδNRlθC(NCN)SR9, CRδRδNR 10C(CR4NO2)SR9, CRδRδC(O)ORi4, CRδRδC(Y')NRioRl4, CRδRδC(NRιo)NRιoRl4, CRδRδCN, CRδRδ(tetrazolyl), CRδRδ(imidazolyl), CRδRδ(imidazolidinyl), CRδRδ(pyrazolyl), CRδRδ( hiazolyl), CRδRδ(thiazolidinyl), CRδRδ(oxazolyl), CRδRδ(oxazolidinyl), CRδRδ(triazolyl), CRδRδ(isoxazolyl),

CRδRδ(oxadiazolyl), CRδRδ( hiadiazolyl), CRδR8(π-oφholinyl), CRδRδ(piperidinyl), CRδRδ(piperazinyl), CRδRδ(pyrrolyl), CRδRδC(NORδ)Rl4, CRδRδC(NORi4)Rδ, CRδRδNRιoC(NRιo)SR9, CRδRδNRioC(NRιo)NRιoRl4, CRδRδNRiθC(O)C(O)NRιoRl4, or CR RδNR ιoC(O)C(O)OR 14; X5 is H, R9, ORδ, CN, C(O)Rδ, C(O)ORδ, C(O)NRδRδ, or NRδRδ;

X₄ is H, R9, ORδ, CN, C(O)Rδ, C(O)ORδ, C(O)NRδRδ, or NRδRδ;

Y' is O or S;

R7 is -(CR4R5)qRi2 or Ci.g alkyl wherein the R12 or Cι_6 alkyl group is unsubstituted or substituted one or more times by methyl or ethyl unsubstituted or substituted by 1-3 fluorines, -F, -Br, -Cl, -NO2, -NRjoRl l , -C(O)Rδ, -Cθ2R ,

-O(CH₂)_qRδ, -CN, -C(O)NRioRl 1, -O(CH₂)_qC(O)NRιoRl l, -O(CH₂)_qC(O)R₉, -NRιoC(O)NRioRl l, -NRιoC(O)Rn, -NRιoC(O)OR9, -NRιoC(O)Ri3, -C(NRιo)NRιoRl l, -C(NCN)NRιoRl l, -C(NCN)SR9, -NRioC(NCN)SR9 , -NRιoC(NCN)NRioRl 1, -NRiθS(O)2R9, -S(O)_m'R9, -NRiθC(O)C(O)NRιoRl 1, - NRιoC(O)C(O)Rιo, or R₁₃; q is O, 1, or 2;

Rl2 is R13, C3-C7 cycloalkyl, or an unsubstituted or substituted aryl or heteroaryl group selected from the group consisting of (2-, 3- or 4-pyridyl), pyrimidyl, pyrazolyl, (1- or 2-imidazolyl), pyrrolyl, piperazinyl, piperidinyl, moφholinyl, furanyl, (2- or 3-thienyl), quinolinyl, naphthyl, and phenyl;

Rl2 is selected from R13, C3-7 cycloalkyl, (2-, 3- or 4-pyridyl), pyrimidyl, pyrazolyl, (1- or 2-imidazolyl), pyrrolyl, piperazinyl, piperidinyl, moφholinyl, furanyl, (2- or 3-thienyl), quinolinyl, naphthyl, or phenyl;

Rδ is hydrogen or R9; R9 is C}_4 alkyl optionally substituted by one to three fluorines;

Rl_Q is ORδ or Rn;

Rl is hydrogen, or C1.4 alkyl optionally substituted by one to three fluorines; or when Rio and Rl 1 are as NRio l 1 they may together with the nitrogen form a 5 to 7 membered ring comprised of carbon or carbon and at least one additional heteroatom selected from O, N, or S;

Rl3 is a substituted or unsubstituted heteroaryl group selected from the group consisting of oxazolidinyl, oxazolyl, thiazolyl, pyrazolyl, triazolyl, tetrazolyl, imidazolyl, imidazolidinyl, thiazolidinyl, isoxazolyl, oxadiazolyl, and thiadiazolyl, and where R-₃ is substituted on R-₂ or R_i3 the rings are connected through a carbon atom and each second R_]3 ring may be unsubstituted or substituted by one or two C _2 alkyl groups unsubstituted or substituted on the methyl with 1 to 3 fluoro atoms;

Rl4 is hydrogen or R7; or when Rδ and R14 are as NRδRl4 they may together with the nitrogen form a 5 to 7 membered ring comprised of carbon or carbon and one or more additional heteroatoms selected from O, N, or S;

R₁₅ is C(O)Ri4, C(O)NR4Rl4, S(O)2R7, or S(O)2NR4Rl4; provided that:

(f) R7 is not Cj-4 alkyl optionally substituted by one to three fluorines; or the pharmaceutically acceptable salts thereof. 2. A compound according to claim 1 wherein K\ is -CH2-cyclopropyl,

-CH2-C5-6 cycloalkyl, -C4-6 cycloalkyl unsubstituted or substituted by OH, tetrahydrofuran-3-yl, (3- or 4-cyclopentenyl), benzyl or -Ci-2 alkyl unsubstituted or substituted by 1 or more fluorines, and -(CH2)2-4 OH; R2 is methyl or fluoro- substituted alkyl, W is ethynyl or 1 ,3-butadiynyl; R₃ is R₇ where R is an unsubstituted or substituted aryl or heteroaryl ring, X is YR2, and Z is CRδ δORlS, or

CRδR8NRlθ l4.

3. A compound according to claim 2 wherein Ri is -CH2-cyclopropyl, cyclopentyl, 3-hydroxycyclopentyl, methyl or CF2H; X is YR2; Y is oxygen; X2 is oxygen; X3 is hydrogen; and R2 is CF2H or methyl, W is ethynyl or 1 ,3-butadiynyl, and R3 is a substituted or unsubstituted pyrimidinyl ring.

4. A compound according to claim 2 which is cw-[3-(3-cyclopentyloxy-4-methoxyphenyl)-3-(4-pyridylethynyl)- 1 -

(hydroxymethyl)cyclohexane], or cw-[3-(3-cyclopentyloxy-4-methoxyphenyl)-3-(4-pyridylethynyl)- 1 - (aminomethyl)cyclohexane].

5. A pharmaceutical composition comprising a compound of Formula I according to claim 1 and a pharmaceutically acceptable excipient.