EP0729569A1 - Apparatus for determining the erythrocyte sedimentation rate - Google Patents
Apparatus for determining the erythrocyte sedimentation rateInfo
- Publication number
- EP0729569A1 EP0729569A1 EP95901660A EP95901660A EP0729569A1 EP 0729569 A1 EP0729569 A1 EP 0729569A1 EP 95901660 A EP95901660 A EP 95901660A EP 95901660 A EP95901660 A EP 95901660A EP 0729569 A1 EP0729569 A1 EP 0729569A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- blood
- sample
- determining
- esr
- measuring cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 210000003743 erythrocyte Anatomy 0.000 title claims abstract description 13
- 238000004062 sedimentation Methods 0.000 title abstract description 5
- 210000004369 blood Anatomy 0.000 claims abstract description 86
- 239000008280 blood Substances 0.000 claims abstract description 86
- 210000004027 cell Anatomy 0.000 claims abstract description 38
- 238000005534 hematocrit Methods 0.000 claims abstract description 18
- 239000003085 diluting agent Substances 0.000 claims description 20
- 238000000034 method Methods 0.000 claims description 9
- 238000012937 correction Methods 0.000 claims description 4
- 210000000601 blood cell Anatomy 0.000 claims description 3
- 238000007865 diluting Methods 0.000 claims 1
- 238000005065 mining Methods 0.000 claims 1
- 239000000523 sample Substances 0.000 description 15
- 238000012360 testing method Methods 0.000 description 11
- 210000002381 plasma Anatomy 0.000 description 4
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 3
- 239000003146 anticoagulant agent Substances 0.000 description 3
- 229940127219 anticoagulant drug Drugs 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000012470 diluted sample Substances 0.000 description 2
- 210000003617 erythrocyte membrane Anatomy 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- ULGZDMOVFRHVEP-RWJQBGPGSA-N Erythromycin Chemical compound O([C@@H]1[C@@H](C)C(=O)O[C@@H]([C@@]([C@H](O)[C@@H](C)C(=O)[C@H](C)C[C@@](C)(O)[C@H](O[C@H]2[C@@H]([C@H](C[C@@H](C)O2)N(C)C)O)[C@H]1C)(C)O)CC)[C@H]1C[C@@](C)(OC)[C@@H](O)[C@H](C)O1 ULGZDMOVFRHVEP-RWJQBGPGSA-N 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000000701 coagulant Substances 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- GICLSALZHXCILJ-UHFFFAOYSA-N ctk5a5089 Chemical compound NCC(O)=O.NCC(O)=O GICLSALZHXCILJ-UHFFFAOYSA-N 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002847 impedance measurement Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000010363 phase shift Effects 0.000 description 1
- 230000011218 segmentation Effects 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
- G01N33/491—Blood by separating the blood components
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/04—Investigating sedimentation of particle suspensions
- G01N15/05—Investigating sedimentation of particle suspensions in blood
Definitions
- This invention concerns determination of the erythrocyte sedimentation rate, ESR, using an empirical relationship bet ⁇ ween the ESR value and certain electrically measured quanti- ties of the blood sample the ESR value of which is sought. Determination of the ESR value is a common diagnostic step. Usually, the ESR value is determined by first aspirat ⁇ ing an anticoagulated sample of the patient's blood into a narrow transparent tube so that the tube is filled to a pre- determined height, and then leaving the sample undisturbed for a certain time, typically one hour, whereupon the height of the more or less colourless supernatant column of blood plasma formed as a result of the sedimentation of the red blood cells is measured. The ESR value . ' equal to the height of this column in millimetres if the segmentation time is one hour.
- the rather long time required to arrive at the ESR value using this technique is a serious drawback, and the object of this invention is to render possible a rapid determination of the ESR value using only a small volume of blood.
- an apparatus for determining the ESR value of a sample of blood which has the characterising features set forth in the independent claim.
- the dependent claims recite features of preferred embodiments of the apparatus.
- Fig. 1 is a diagrammatic view of a first embodiment which incorporates a particle counter used for determining the haematocrit of the blood;
- Fig. 2 is a diagrammatic view of a second embodiment in which the haematocrit is determined from the impedance of the blood;
- Fig. 3 is a diagrammatic view showing a modification of the embodiment shown in Fig. 2.
- a tube 2 which extends into the sample in the test tube 1 is connected to a measuring cell 3 provided with four electrodes, two outer electrodes 4 and two inner elec- trodes 5 positioned between the outer electrodes.
- blood is aspirated from the test tube 1 into the measuring cell and a current is passed through the blood in the measuring cell 3 by way of the outer electrodes 4.
- the voltage then produced between the two inner electrodes 5 is measured, and circuitry generally designated 6 determines the electrical impedance of the blood in accordance with well- known techniques.
- the current may be fed to the outer elec ⁇ trodes 4 at three different frequencies (the highest of which should be many times higher than the lowest) ; this permits simultaneous determination of the capacitance C of the blood and the resistance R of the blood plasma and at the same eli ⁇ minates errors due to the interior resistance of the erythro- cytes.
- a further possibility is to use known methods involv- ing only two frequencies and a measurement of phase shift in order to determine C and R.
- the blood is transported through the tube 2 and the mea ⁇ suring cell 3 by means of a pump 7.
- Another pump 8 feeds a liquid diluent from a reservoir 9 into the tube downstream of the measuring cell 3 to dilute the blood before the blood is passed through a very narrow and short passage 10 which forms part of an electronic blood cell counter of a well-known type.
- This blood cell counter comprises a pair of electrodes 11 which are positioned on opposite sides of the passage 10 and connected with circuitry 12 for counting the erythrocytes and determining the haematocrit of the blood.
- Determination of the ESR value of the blood is effected by means of circuitry 15 using an empirical relationship of the kind described below.
- a display 16 indicates the deter ⁇ mined ESR value.
- the circuitry 16 processes the inputs recei ⁇ ved from the impedance determination circuitry 6, the haema ⁇ tocrit determination circuitry 12 and the temperature deter ⁇ mination circuitry 14.
- the quantity is not very critical but the volume of added diluent should not exceed the volume of the undiluted blood sample.
- a preferred ratio of the volume of diluent to the volume of undiluted blood is 1/4 to 1/2 (the volume of anti ⁇ coagulant is included in the volume of diluent) .
- diluent to the blood may be effected from the diluent reservoir 9 by means of the pump 8.
- a separate diluent pump may be provided as described below.
- the addition of a small quantity of diluent is advanta ⁇ geous in that it inhibits certain saturation effects which have been found to occur with undiluted blood and affect the precision of the determination for high ESR values. It is important that only a small amount of diluent is added, be ⁇ cause the addition of a diluent also has been found to impose requirements for an increased accuracy of the ESR determina ⁇ tion circuitry 15. The dilution can be dispensed with if ESR values in the high range need not be determined very accura ⁇ tely.
- a diluent of low electrical conductivity such as an aqueous solution of glycine (monoaminoacetic acid) .
- the diluent should be isotonic so that any change of volume of the erythrocytes is avoided, or the determination of the haematocrit should be carried out on undiluted blood.
- the portion of the blood sample used for de- termining the haematocrit need not pass through the measuring cell 3; this portion of the blood may be fed to the cell counter through a separate line.
- a separate double pump 23 is provided to draw a precisely measured volume of blood from the test tube 1 through a narrow tube 22 and a precisely measured volume of diluent from a diluent reservoir 24 and to feed the blood and the diluent to a vessel 25 in which a thorough mixing is effected by means a suitable mixing device, not shown.
- pump 7 draws the diluted blood through tube 2 into the measuring cell 3 which is provided with a pair of outer electrodes 4 and a pair of inner electrodes 5. These electrodes are con ⁇ nected to impedance determination circuitry 6 which feeds its output to ESR determination circuitry 15. The latter also re- ceives the output of temperature measuring circuitry 14 and drives display 16.
- Fig. 3 illustrates a modification of the means for pro ⁇ viding a diluted sample to be drawn into measuring cell 3. Apart from this modification, the embodiment of Fig. 3 is identical with that shown in Fig. 2.
- the blood sample is taken from the patient into a special cylindrical test tube 36 having a closure 37 through which a hollow needle may be passed.
- the test tube 36 is filled with a predeter- mined volume of diluent and an anticoagulant.
- the test tube 36 is positioned in a holder 38 associated with a level indicator comprising a light source 39 and an array of photosensitive elements 40 on a scale 41.
- a computing circuit 42 connected to the level indicator 39-41 determines the total volume of the diluted sample in the test tube 36 and the degree of dilution.
- the output of circuit 42 is fed to ESR determination circuitry 15 (not shown in Fig. 3, see instead Fig. 2).
- a sealed test tube w ich is pre-filled with a predeter ⁇ mined volume of diluent (including an anticoagulant) and the interior of which is under a partial vacuum.
- diluent including an anticoagulant
- the diluted blood in the test tube 36 is fed to the mea ⁇ suring cell through a hollow needle 43 and tube 2.
- the haematocrit of the blood is determined from impedance measu ⁇ rements by means of the electrodes 4 and 5, and as in the embodiment of Fig. 1 the ESR value is determined using an empirical relationship. A number of examples of relatio "hips for use in determining the ESR value will be given herein ⁇ after.
- the determination as described herein ⁇ after comprises three steps: (1) correction for varying sample temperature, (2) determination of the haematocrit, and (3) determination of the ESR value. Temperature correction of the measured impedances can be accomplished using the empirical formula
- Z 0 is the normalised value of a certain impedance value Z t measured at temperature t in relation to a certain reference temperature t 0 for which the following empirical formulas have been established.
- Constant c is a constant for the temperature dependency of the impedances; this dependency varies slightly with the frequency of the current.
- the haematocrit H can be measured by means of a cell counter as in the embodiment of Fig. 1.
- the haematocrit H expressed as a volume percentage, is calculated using an empirical relationship which includes resistances measured at different frequencies and ratios of these resistances, such as the following relationship which includes the ratios rj and r 2 :
- R lf R 2 and R 3 are the resistances of blood anticoagulated with EDTA, as determined at frequencies of 100 kHz, 800 kHz and 1200 kHz.
- a rough ESR value can be calculated from the following empirical relationship
- C is the capacitance as measured at the second point in time
- the apparatus of Fig. 2 includes or is associated with a cell counter supplying the number of at least one type of cells in the blood, such as the number of white cells and/or the number of erythrocytes
- the ESR value can be calculated using a relationship of the following type
- B is the number of erythrocytes per 10 "12 litre of blood
- constant c 20 -0.078
- constant c 21 0.084
- constant c 22 -0.463
- constant c 23 l.973
- constant c 24 -0.0108
- constant c 25 -o.267
- constant c 26 1.974
- constant c 27 -11.03
- Determination of the ESR value using the method and the apparatus according to the invention requires only a relati ⁇ vely small volume of the sample of blood, 1 ml or less.
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
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Abstract
Apparatus for determining the erythrocyte sedimentation rate, ESR, of a sample of blood comprises a measuring cell (3) receptive of the sample of blood, electrical means (4-6) connected with the measuring cell for determining the capacitance (C) of the sample of blood received in the measuring cell (3), means (10-12; 15) for determining the haematocrit of the sample of blood, and electrical means (15) for determining the ESR using an empirical relationship expressing the ESR as a function of the capacitance (C) and the haematocrit (H) of the sample of blood.
Description
Apparatus for determining the erythrocvte sedimentation rate
This invention concerns determination of the erythrocyte sedimentation rate, ESR, using an empirical relationship bet¬ ween the ESR value and certain electrically measured quanti- ties of the blood sample the ESR value of which is sought. Determination of the ESR value is a common diagnostic step. Usually, the ESR value is determined by first aspirat¬ ing an anticoagulated sample of the patient's blood into a narrow transparent tube so that the tube is filled to a pre- determined height, and then leaving the sample undisturbed for a certain time, typically one hour, whereupon the height of the more or less colourless supernatant column of blood plasma formed as a result of the sedimentation of the red blood cells is measured. The ESR value .' equal to the height of this column in millimetres if the segmentation time is one hour.
The rather long time required to arrive at the ESR value using this technique is a serious drawback, and the object of this invention is to render possible a rapid determination of the ESR value using only a small volume of blood.
To this end, there is provided in accordance with the invention an apparatus for determining the ESR value of a sample of blood which has the characterising features set forth in the independent claim. The dependent claims recite features of preferred embodiments of the apparatus.
As is previously known, the electrical impedance of blood is primarily determined by plasma resistance, erythro¬ cyte interior fluid resistance and erythrocyte membrane capa¬ citance. We have found that the ESR of blood is related to elec¬ trical impedance parameters of the blood, especially the erythrocyte membrane capacitance, and that an alternative and fast method for determination of the ESR value can be based on impedance measurement. The invention will be described in greater detail here¬ inafter, reference being had to the accompanying diagrammatic drawings, in which:
Fig. 1 is a diagrammatic view of a first embodiment which incorporates a particle counter used for determining the haematocrit of the blood;
Fig. 2 is a diagrammatic view of a second embodiment in which the haematocrit is determined from the impedance of the blood;
Fig. 3 is a diagrammatic view showing a modification of the embodiment shown in Fig. 2.
Referring to Fig. 1, the sample of blood the ESR value of which is to be determined and to which an anticoagulant has been added, is received in a suitable vessel, such as a test tube 1. A tube 2 which extends into the sample in the test tube 1 is connected to a measuring cell 3 provided with four electrodes, two outer electrodes 4 and two inner elec- trodes 5 positioned between the outer electrodes. In use of the apparatus, blood is aspirated from the test tube 1 into the measuring cell and a current is passed through the blood in the measuring cell 3 by way of the outer electrodes 4. The voltage then produced between the two inner electrodes 5 is measured, and circuitry generally designated 6 determines the electrical impedance of the blood in accordance with well- known techniques.
For example, the current may be fed to the outer elec¬ trodes 4 at three different frequencies (the highest of which should be many times higher than the lowest) ; this permits simultaneous determination of the capacitance C of the blood and the resistance R of the blood plasma and at the same eli¬ minates errors due to the interior resistance of the erythro- cytes. A further possibility is to use known methods involv- ing only two frequencies and a measurement of phase shift in order to determine C and R.
The blood is transported through the tube 2 and the mea¬ suring cell 3 by means of a pump 7. Another pump 8 feeds a liquid diluent from a reservoir 9 into the tube downstream of the measuring cell 3 to dilute the blood before the blood is passed through a very narrow and short passage 10 which forms part of an electronic blood cell counter of a well-known type. This blood cell counter comprises a pair of electrodes
11 which are positioned on opposite sides of the passage 10 and connected with circuitry 12 for counting the erythrocytes and determining the haematocrit of the blood.
A temperature sensing member 13, such as a thermistor, is associated with the measuring cell 3 and connected to cir¬ cuitry 14 for determining th-*- temperature of the blood in the measuring cell.
Determination of the ESR value of the blood is effected by means of circuitry 15 using an empirical relationship of the kind described below. A display 16 indicates the deter¬ mined ESR value. The circuitry 16 processes the inputs recei¬ ved from the impedance determination circuitry 6, the haema¬ tocrit determination circuitry 12 and the temperature deter¬ mination circuitry 14. Before the blood sample is aspirated into the measuring cell 3 it should be slightly diluted by the addition of a predetermined, relatively small quantity of a liquid diluent. The quantity is not very critical but the volume of added diluent should not exceed the volume of the undiluted blood sample. A preferred ratio of the volume of diluent to the volume of undiluted blood is 1/4 to 1/2 (the volume of anti¬ coagulant is included in the volume of diluent) .
The addition of the diluent to the blood may be effected from the diluent reservoir 9 by means of the pump 8. Alterna- tively, a separate diluent pump may be provided as described below.
The addition of a small quantity of diluent is advanta¬ geous in that it inhibits certain saturation effects which have been found to occur with undiluted blood and affect the precision of the determination for high ESR values. It is important that only a small amount of diluent is added, be¬ cause the addition of a diluent also has been found to impose requirements for an increased accuracy of the ESR determina¬ tion circuitry 15. The dilution can be dispensed with if ESR values in the high range need not be determined very accura¬ tely.
Preferably, a diluent of low electrical conductivity is used, such as an aqueous solution of glycine (monoaminoacetic
acid) . The diluent should be isotonic so that any change of volume of the erythrocytes is avoided, or the determination of the haematocrit should be carried out on undiluted blood. Naturally, the portion of the blood sample used for de- termining the haematocrit need not pass through the measuring cell 3; this portion of the blood may be fed to the cell counter through a separate line.
In the embodiment shown in Fig. 2 a separate double pump 23 is provided to draw a precisely measured volume of blood from the test tube 1 through a narrow tube 22 and a precisely measured volume of diluent from a diluent reservoir 24 and to feed the blood and the diluent to a vessel 25 in which a thorough mixing is effected by means a suitable mixing device, not shown. As in the previous embodiment, pump 7 draws the diluted blood through tube 2 into the measuring cell 3 which is provided with a pair of outer electrodes 4 and a pair of inner electrodes 5. These electrodes are con¬ nected to impedance determination circuitry 6 which feeds its output to ESR determination circuitry 15. The latter also re- ceives the output of temperature measuring circuitry 14 and drives display 16.
Fig. 3 illustrates a modification of the means for pro¬ viding a diluted sample to be drawn into measuring cell 3. Apart from this modification, the embodiment of Fig. 3 is identical with that shown in Fig. 2.
In this case, the blood sample is taken from the patient into a special cylindrical test tube 36 having a closure 37 through which a hollow needle may be passed. Before the blood sample is taken, the test tube 36 is filled with a predeter- mined volume of diluent and an anticoagulant. After the blood sample has been taken, the test tube 36 is positioned in a holder 38 associated with a level indicator comprising a light source 39 and an array of photosensitive elements 40 on a scale 41. A computing circuit 42 connected to the level indicator 39-41 determines the total volume of the diluted sample in the test tube 36 and the degree of dilution. The output of circuit 42 is fed to ESR determination circuitry 15 (not shown in Fig. 3, see instead Fig. 2).
It ,.s also possible to obtain the desired dilution by using a sealed test tube w ich is pre-filled with a predeter¬ mined volume of diluent (including an anticoagulant) and the interior of which is under a partial vacuum. When the blood sample is taken, blood is allowed to flow into the test tube until pressure equilibrium has been reached, i.e. until a predetermined volume of blood has been introduced.
The diluted blood in the test tube 36 is fed to the mea¬ suring cell through a hollow needle 43 and tube 2. In the embodiment shown in Fig. 2 and Fig. 3, the haematocrit of the blood is determined from impedance measu¬ rements by means of the electrodes 4 and 5, and as in the embodiment of Fig. 1 the ESR value is determined using an empirical relationship. A number of examples of relatio "hips for use in determining the ESR value will be given herein¬ after.
As will become apparent from the examples, most of which are based on determination of the electrical impedance of the blood at three different frequencies, different degrees of complexity in the determination can be required, depending on the required accuracy. The determination as described herein¬ after comprises three steps: (1) correction for varying sample temperature, (2) determination of the haematocrit, and (3) determination of the ESR value. Temperature correction of the measured impedances can be accomplished using the empirical formula
Z0 = ZJ1 + c(t-g]
in which Z0 is the normalised value of a certain impedance value Zt measured at temperature t in relation to a certain reference temperature t0 for which the following empirical formulas have been established. Constant c is a constant for the temperature dependency of the impedances; this dependency varies slightly with the frequency of the current.
The haematocrit H can be measured by means of a cell counter as in the embodiment of Fig. 1. In the embodiment of Fig. 2 the haematocrit H, expressed as a volume percentage, is calculated using an empirical relationship which includes
resistances measured at different frequencies and ratios of these resistances, such as the following relationship which includes the ratios rj and r2:
H = c1R1 + c2R3 + 03^ + c4r2 + c5
in which, for a temperature of 26°C, constant c^-0.0223, constant c2=0.200, constant c3=122,8, constant c4=-172.2 and constant c5=51.3, and Rlf R2 and R3 are the resistances of blood anticoagulated with EDTA, as determined at frequencies of 100 kHz, 800 kHz and 1200 kHz.
A rough ESR value can be calculated from the following empirical relationship
ln(ESR) = _?- + c, H07
in which C is the capacitance of the diluted blood, H2 is the haematocrit, expressed as a percentage by volume, and con¬ stant c6=0.98, constant c7=l and constant c8=-7.14 at 37°C when citrate has been added to the blood to prevent coagulation. A more accurate value is obtained if the resistance R of plasma is taken into consideration using the following empir¬ ical relationship
ln(ESR) = -^55 + Cl1
|_| Cl0
in which constant c9=0,207, constant c10=2 and constant cn=-4,62. A further improvement of the accuracy is achieved if the difference, ΔC, between the capacitance values measured at two different points in time after the diluted blood has been aspirated into measuring cell 3 and become stationary, and if corrections for the haematocrit are also introduced. Then, the following empirical relationship can be used:
iInn/(PfcSQmR) = C 2°2 +— C 3ΔC 2 ++ c14R ÷ c15C + c16ΔC + c17H + c18H + c19
in which C is the capacitance as measured at the second point in time, constant Cι2=-0.0646, constant Cι3=0.0166, constant c14=0.414, constant Cι5=1.836, constant c16=0,283, constant c17=- 0.0066, constant clg=0.397 and constant c19=-18.27 when the points in time for the determination of ΔC are 2 and 90 seconds after the blood has been aspirated, the blood has been anticoagulated with EDTA and diluted with one part of a 0,1 mol/1 saline per three parts of blood, and the measure¬ ments have been carried out at 26°C. If the apparatus of Fig. 2 includes or is associated with a cell counter supplying the number of at least one type of cells in the blood, such as the number of white cells and/or the number of erythrocytes, the ESR value can be calculated using a relationship of the following type
■nCESB) ■ *-°* ^ » -■» » ■ - * *+0 r cB* . o^B - _-
in which B is the number of erythrocytes per 10"12 litre of blood, constant c20=-0.078, constant c21=0.084, constant c22=-0.463, constant c23=l.973, constant c24=-0.0108, constant c25=-o.267, constant c26=1.974 and constant c27=-11.03 when the blood has been anticoagulated with EDTA and diluted with one part of a 0,1 mol/1 saline per three parts of blood, and the measurements have been carried out at 26°C.
The influence of the resistance of the interior fluid of the erythrocytes has been found not to have a significant in¬ fluence on the ESR value. It therefore need not be taken into account.
The various constants of the empirical relationships can readily be determined from a number of blood samples of known ESR values.
Determination of the ESR value using the method and the apparatus according to the invention requires only a relati¬ vely small volume of the sample of blood, 1 ml or less.
Claims
1. Apparatus for determining the erythrocyte sedimen¬ tation rate, ESR, of a sample of blood, characterised by a measuring cell (3) having a compartment receptive of the sample of blood, electrical means (4-6) connected with the measuring cell for determining the capacitance C of the sample of blood received in the measuring cell (3) , means (10-12; 15) for determining the haematocrit of the sample of blood, and electrical means (15) for determining the ESR using an empirical relationship expressing the ESR as a func¬ tion of the capacitance C and the haematocrit H of the sample of blood.
2. Apparatus according to claim 1, characterised in that said relationship is of the following type: c„C ln(ESR) = -i- - c8
in which In(ESR) is the natural logarithm of the ESR value ex¬ pressed in mm/h, C is the capacitance of the blood in the measuring cell, H is the haematocrit value of the blood, ex¬ pressed as a percentage by volume, and c6, c7 and c8 are con¬ stants.
3. Apparatus according to claim 1 or 2, characterised in that said electrical means (4-6) for determining the capa¬ citance C includes means for determining the electrical resis¬ tance R of the plasma of the sample of blood and said rela¬ tionship is of the following type:
ln(ESR) = -^5 + cn H Cιo
in which c9, c10 and cn are constants.
4. Apparatus according to any one of claims 1 to 3, characterised in that said electrical means (15) for deter¬ mining the ESR is associated with means for determining the temperature t of the blood received in the measuring cell (3) and in that the said relationship includes R as a factor for correction for the deviation of t from the temperature t0 for which the constants have been determined.
5. Apparatus according to any one of claims 1-4, characterised in that said means for determining the haemato¬ crit H comprises an electronic cell counter (10-12) connected with said ele .rical means (15) for determining the ESR.
6. Apparatus according to any one of claims 1-4, characterised in that said electrical means (4-6) connected with the measuring cell (3) for determining the capacitance C of the sample of blood received in the measuring cell inclu¬ des means for determining for three frequencies of an elec- trie current passed through the sample of blood received in the measuring cell both the capacitance C and the resistance R of the plasma of the sample of blood, and in that said means for determining the haematocrit H of the sample of blood comprises electrical means for determining H from the measured values of R.
7. Apparatus according to any one of claims 1-6, characterised in that said relationship includes the differ¬ ence ΔC in the capacitances of the blood measured at two dif¬ ferent times after the sample of blood has been received in the measuring cell (3) .
8. Apparatus according to claim 7, characterised in that said relationship is of the following type:
ln(ESR) . C"C 2 ÷ C"ΔC 2 r °»* » lfi * CAC f ^ + ^ ^ 0]9 H rl
in which C is the capacitance as measured at the second point in time, constant Cj2, c13, c14, c15, c16, c17, c18 and c19 are constants.
9. Apparatus according to any one of claims 1-8, characterised in that a blood cell counter is associated with sa electrical means (15) for determining the ESR and in that said relationship includes the number of one or more types of cells, such as the number of white cells and/or the number of erythrocytes, per unit volume of the sample of blood.
10. Apparatus according to any one of claims 1-9, characterised by a device (23,24; 38-42) for diluting the sample of blood with a predetermined volume of diluent before the sample of blood is received in the measuring cell (3) , said predetermined volume preferably not exceeding the volume of the sample of blood before it is diluted.
11. A method for determining the erythrocyte sedi en- tation rate, ESR, of a sample of blood, comprising the steps of introducing the sample of blood in a measuring cell (3) , determining by electrical means the capacitance C of the sample of blood in the measuring cell, determining the haema¬ tocrit of the sample of blood, and determining by electrical means the ESR using an empirical relationship expressing the ESR as a function of the capacitance C and the haematocrit H of the sample of blood.
12. A method according to claim 11, characterised in that the difference ΔC in the capacitances of the blood is measured at two different times after the sample of blood has been received in the measuring cell (3) and that the said relationship includes said difference.
13. A method according to claim 11 or 12, characterised in that the sample of- blood is diluted with a predetermined volume of diluent before it is introduced in the measuring cell, said volume preferably not exceeding the volume of the sample of blood before it is diluted.
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE9303751A SE507564C2 (en) | 1993-11-15 | 1993-11-15 | Appts. for rapid measurement of erythrocyte sedimentation rate |
SE9303751 | 1993-11-15 | ||
SE9401646A SE507563C2 (en) | 1993-11-15 | 1994-05-11 | Device for determining blood lowering reaction |
SE9401646 | 1994-05-11 | ||
PCT/SE1994/001075 WO1995014224A1 (en) | 1993-11-15 | 1994-11-15 | Apparatus for determining the erythrocyte sedimentation rate |
Publications (1)
Publication Number | Publication Date |
---|---|
EP0729569A1 true EP0729569A1 (en) | 1996-09-04 |
Family
ID=26661891
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP95901660A Withdrawn EP0729569A1 (en) | 1993-11-15 | 1994-11-15 | Apparatus for determining the erythrocyte sedimentation rate |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP0729569A1 (en) |
JP (1) | JPH09505146A (en) |
CN (1) | CN1135258A (en) |
AU (1) | AU1080795A (en) |
SE (1) | SE507563C2 (en) |
WO (1) | WO1995014224A1 (en) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE69942146D1 (en) * | 1998-03-19 | 2010-04-29 | Inverness Medical Switzerland | DEVICE FOR DETERMINING BLOOD COAGULATION by means of resistance measurements |
DE10210009B3 (en) * | 2002-03-07 | 2004-01-08 | Fresenius Medical Care Deutschland Gmbh | Method for determining the hematocrit and / or blood volume and device for extracorporeal blood treatment with a device for determining the hematocrit and / or blood volume |
DE10321099A1 (en) * | 2003-05-09 | 2004-11-25 | Cgs Sensortechnik Gmbh | Pressure measuring device |
ITUD20030174A1 (en) | 2003-09-03 | 2005-03-04 | Sire Analytical Systems Srl | INTEGRATED SYSTEM FOR HEMATOLOGICAL ANALYSIS AND ITS METHOD. |
KR100844532B1 (en) * | 2006-12-28 | 2008-07-08 | 한국기계연구원 | Erythrocyte sedimentation rate log |
JP2014115256A (en) * | 2012-12-12 | 2014-06-26 | Sony Corp | Container for electrical measurement, apparatus for electrical measurement, and method for electrical measurement |
JP6360696B2 (en) * | 2014-03-18 | 2018-07-18 | 日本光電工業株式会社 | Blood test apparatus and blood test method |
CN107884563A (en) * | 2017-11-26 | 2018-04-06 | 张延艳 | A kind of medical treatment device and its application method for blood analysis |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB1036932A (en) * | 1962-05-03 | 1966-07-20 | Noeller Hans Guenter | Blood sedimentation apparatus |
FR2201762A5 (en) * | 1972-09-29 | 1974-04-26 | Inst Nat Sante Rech Med | |
SE465140B (en) * | 1989-12-13 | 1991-07-29 | Tesi Ab | PROCEDURE AND DEVICE TO DETERMINE BLOOD RECONCILIATION REACTION |
EP0630471A1 (en) * | 1992-03-10 | 1994-12-28 | BARNS, Christopher | Apparatus for determining the physical and/or chemical properties of a sample, particularly of blood |
-
1994
- 1994-05-11 SE SE9401646A patent/SE507563C2/en not_active IP Right Cessation
- 1994-11-15 EP EP95901660A patent/EP0729569A1/en not_active Withdrawn
- 1994-11-15 JP JP7514389A patent/JPH09505146A/en active Pending
- 1994-11-15 WO PCT/SE1994/001075 patent/WO1995014224A1/en not_active Application Discontinuation
- 1994-11-15 AU AU10807/95A patent/AU1080795A/en not_active Abandoned
- 1994-11-15 CN CN 94194142 patent/CN1135258A/en active Pending
Non-Patent Citations (1)
Title |
---|
See references of WO9514224A1 * |
Also Published As
Publication number | Publication date |
---|---|
CN1135258A (en) | 1996-11-06 |
SE9401646D0 (en) | 1994-05-11 |
JPH09505146A (en) | 1997-05-20 |
WO1995014224A1 (en) | 1995-05-26 |
AU1080795A (en) | 1995-06-06 |
SE9401646L (en) | 1995-05-16 |
SE507563C2 (en) | 1998-06-22 |
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