EP0563240A1 - Lipid receptors for microorganisms and corresponding adhesins, dna sequences encoding adhesins, and use thereof - Google Patents
Lipid receptors for microorganisms and corresponding adhesins, dna sequences encoding adhesins, and use thereofInfo
- Publication number
- EP0563240A1 EP0563240A1 EP19920902654 EP92902654A EP0563240A1 EP 0563240 A1 EP0563240 A1 EP 0563240A1 EP 19920902654 EP19920902654 EP 19920902654 EP 92902654 A EP92902654 A EP 92902654A EP 0563240 A1 EP0563240 A1 EP 0563240A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- protein
- receptor
- adhesin protein
- adhesin
- microorganism
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/205—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Campylobacter (G)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/295—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Chlamydiales (O)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
Definitions
- the present invention provides a variety of compositions and methods related to lipid receptors for microorganisms, the complementary microbial adhesins, and PNA seguences encoding adhesins.
- the present invention provides an isolated PNA molecule encoding an adhesin protein.
- the PNA molecule is cPNA.
- the PNA molecule is geno ic PNA.
- an isolated PNA molecule capable of specifically hybridizing with a PNA molecule encoding an adhesin protein.
- Suitable reporter groups for direct or indirect detection include radioisotopes, fluorophores, enzymes, lumines ⁇ ers, and dye particles. These and other labels are well known in the art and are described, for example, in the following U.S. patents: 3,766,162; 3,791,932; 3,817,837; 3,996,345; and 4,233,402.
- the receptor phospholipids described above may be used to isolate a microbial adhesin molecule. Briefly, a representative example of such an isolation begins with a microorganism preparation, such as a membrane extract, which contains an adhesin protein. Membrane extracts may be obtained using standard methodology.
- EBs chlamydial elementary bodies
- Chlamydial organisms were radioiodinated as described for bacteria (Krivan et al., Arch. Biochem. Biophys. 260:493-496. 1988), with minor modifications. Briefly, 0.5 ml of the chlamydial suspension were reacted with 0.5 mCi of Na 125 I at an ice bath in a tube (10 x 75 mm) coated with 0.1 mg of Iodogen (Pierce Chemical Co., Rockford, 111.). After 4 to 5 min, the suspension was transferred to a centrifuge tube containing 5 ml of Tris- BSA buffer (0.05 M Tris hydrochloride [pH 7.8] containing 0.15 M NaCI and 1% bovine serum albumin).
Abstract
L'invention décrit des molécules lipidiques de cellules hôtes (''récepteurs'') permettant la liaison de micro-organismes. On fabrique des compositions pharmaceutiques à partir desdits récepteurs, en combinaison avec un agent porteur ou diluant acceptables pharmaceutiquement. L'invention se rapporte également à des protéines microbiennes (''adhésines''), induisant la liaison des micro-organismes aux cellules hôtes, ainsi qu'à des séquences d'ADN codant les protéines d'adhésine. On fabrique des vaccins à partir d'une protéine d'adhésine en combinaison avec un agent porteur ou un diluant acceptables pharmaceutiquement.The invention describes lipid molecules of host cells ("receptors") allowing the binding of microorganisms. Pharmaceutical compositions are made from said receptors, in combination with a pharmaceutically acceptable carrier or diluent. The invention also relates to microbial proteins ("adhesins"), which induce the binding of microorganisms to host cells, as well as to DNA sequences encoding adhesin proteins. Vaccines are made from an adhesin protein in combination with a pharmaceutically acceptable carrier or diluent.
Description
Claims
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US63237190A | 1990-12-21 | 1990-12-21 | |
US632371 | 1990-12-21 |
Publications (1)
Publication Number | Publication Date |
---|---|
EP0563240A1 true EP0563240A1 (en) | 1993-10-06 |
Family
ID=24535261
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP19920902654 Withdrawn EP0563240A1 (en) | 1990-12-21 | 1991-12-20 | Lipid receptors for microorganisms and corresponding adhesins, dna sequences encoding adhesins, and use thereof |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP0563240A1 (en) |
JP (1) | JPH06504670A (en) |
CA (1) | CA2098733A1 (en) |
WO (1) | WO1992011367A2 (en) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5506139A (en) * | 1994-07-21 | 1996-04-09 | Connaught Laboratories Limited | Analog of haemophilus Hin47 with reduced protease activity |
US5981503A (en) * | 1994-06-07 | 1999-11-09 | Connaught Laboratories Limited | Analog of Haemophilus Hin47 with reduced protease activity |
US5939297A (en) * | 1994-07-21 | 1999-08-17 | Connaught Laboratories Limited | Analog of haemophilus HIN47 with reduced protease activity |
US6147057A (en) * | 1994-07-21 | 2000-11-14 | Connaught Laboratories Limited | Analog of Haemophilus Hin47 with reduced protease activity |
DE69533102T2 (en) * | 1994-07-21 | 2005-06-02 | Aventis Pasteur Ltd., Toronto | ANALOG OF HAEMOPHILUS HIN47 WITH REDUCED PROTEASE ACTIVITY |
CA2223395A1 (en) * | 1995-06-07 | 1996-12-19 | Bjorn L. Mellgard | Nucleic acid and amino acid sequences relating to helicobacter pylori for diagnostics and therapeutics |
AUPR512401A0 (en) * | 2001-05-18 | 2001-06-14 | Queensland University Of Technology | Adherent entities and uses therefor |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DK219084D0 (en) * | 1984-05-02 | 1984-05-02 | Frederik Carl Peter Lindberg | ANTIGEN |
GB8709746D0 (en) * | 1987-04-24 | 1987-05-28 | Wenman W M | Chlamydia vaccine |
-
1991
- 1991-12-20 JP JP4502887A patent/JPH06504670A/en active Pending
- 1991-12-20 CA CA002098733A patent/CA2098733A1/en not_active Abandoned
- 1991-12-20 EP EP19920902654 patent/EP0563240A1/en not_active Withdrawn
- 1991-12-20 WO PCT/US1991/009810 patent/WO1992011367A2/en not_active Application Discontinuation
Non-Patent Citations (1)
Title |
---|
See references of WO9211367A2 * |
Also Published As
Publication number | Publication date |
---|---|
WO1992011367A3 (en) | 1992-10-15 |
JPH06504670A (en) | 1994-06-02 |
WO1992011367A2 (en) | 1992-07-09 |
CA2098733A1 (en) | 1992-06-22 |
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