EP0370103A4 - Protease-deficient gram-positive bacteria and their use as host organisms for the production of recombinant products - Google Patents

Protease-deficient gram-positive bacteria and their use as host organisms for the production of recombinant products

Info

Publication number
EP0370103A4
EP0370103A4 EP19890906977 EP89906977A EP0370103A4 EP 0370103 A4 EP0370103 A4 EP 0370103A4 EP 19890906977 EP19890906977 EP 19890906977 EP 89906977 A EP89906977 A EP 89906977A EP 0370103 A4 EP0370103 A4 EP 0370103A4
Authority
EP
European Patent Office
Prior art keywords
deficient
protease
gram
production
positive bacteria
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP19890906977
Other versions
EP0370103A1 (en
Inventor
Peter Blackburn
Michael Arthur Lonetto
Edward L Chang
June Polak
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Microbiology Inc
Original Assignee
Health Research Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Health Research Institute filed Critical Health Research Institute
Publication of EP0370103A1 publication Critical patent/EP0370103A1/en
Publication of EP0370103A4 publication Critical patent/EP0370103A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/32—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Bacillus (G)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
    • G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
    • G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
    • G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
    • G01N2333/96466—Cysteine endopeptidases (3.4.22)
EP19890906977 1988-05-05 1989-03-14 Protease-deficient gram-positive bacteria and their use as host organisms for the production of recombinant products Withdrawn EP0370103A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US19048388A 1988-05-05 1988-05-05
US190483 1988-05-05

Publications (2)

Publication Number Publication Date
EP0370103A1 EP0370103A1 (en) 1990-05-30
EP0370103A4 true EP0370103A4 (en) 1991-11-27

Family

ID=22701537

Family Applications (1)

Application Number Title Priority Date Filing Date
EP19890906977 Withdrawn EP0370103A4 (en) 1988-05-05 1989-03-14 Protease-deficient gram-positive bacteria and their use as host organisms for the production of recombinant products

Country Status (11)

Country Link
EP (1) EP0370103A4 (en)
JP (1) JPH03500606A (en)
AU (1) AU622916B2 (en)
DK (1) DK1590A (en)
FI (1) FI900045A (en)
HU (1) HUT53154A (en)
IL (1) IL89767A0 (en)
NZ (1) NZ228424A (en)
PT (1) PT90463A (en)
WO (1) WO1989010976A1 (en)
ZA (1) ZA892325B (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5143846A (en) * 1988-03-17 1992-09-01 The General Hospital Corporation Protease deficient bacterial hosts
US5294542A (en) * 1991-03-19 1994-03-15 Omnigene, Inc. Residual protease-III
IL102259A0 (en) * 1991-07-01 1993-01-14 Amgen Inc Isolation and characterization of a protease from streptomyces lividans
US5288931A (en) * 1991-12-06 1994-02-22 Genentech, Inc. Method for refolding insoluble, misfolded insulin-like growth factor-I into an active conformation
AU7788194A (en) 1993-10-05 1995-05-01 Medeva Holdings B.V. Vaccine compositions
GB9324529D0 (en) * 1993-11-30 1994-01-19 Univ Singapore Biological control agents
DE4425645A1 (en) * 1994-07-20 1996-02-22 Mueller Karl & Co Kg New lysostaphin gene with deletion in pro-segment repeat region
EP0998571A2 (en) * 1997-07-15 2000-05-10 Genencor International Inc. Proteases from gram-positive organisms
US6762039B2 (en) * 1997-07-15 2004-07-13 Genencor International, Inc. Bacillus subtillis with an inactivated cysteine protease-1
US6528255B1 (en) 1997-12-30 2003-03-04 Genencor International, Inc. Proteases from gram positive organisms
GB9727470D0 (en) 1997-12-30 1998-02-25 Genencor Int Bv Proteases from gram positive organisms
US6465186B1 (en) 1997-12-30 2002-10-15 Genecor International, Inc. Proteases from gram positive organisms
US6599731B1 (en) 1997-12-30 2003-07-29 Genencor International, Inc. Proteases from gram positive organisms

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987006264A1 (en) * 1986-04-16 1987-10-22 Public Health Research Institute Of The City Of Ne Expression of the cloned lysostaphin gene

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988006624A2 (en) * 1987-02-27 1988-09-07 Gist-Brocades N.V. Molecular cloning and expression of genes encoding proteolytic enzymes

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987006264A1 (en) * 1986-04-16 1987-10-22 Public Health Research Institute Of The City Of Ne Expression of the cloned lysostaphin gene

Also Published As

Publication number Publication date
IL89767A0 (en) 1989-09-28
DK1590A (en) 1990-02-05
FI900045A0 (en) 1990-01-04
HUT53154A (en) 1990-09-28
AU622916B2 (en) 1992-04-30
ZA892325B (en) 1990-03-28
AU3765189A (en) 1989-11-29
HU894054D0 (en) 1990-07-28
NZ228424A (en) 1992-06-25
FI900045A (en) 1990-01-04
JPH03500606A (en) 1991-02-14
PT90463A (en) 1989-11-30
EP0370103A1 (en) 1990-05-30
WO1989010976A1 (en) 1989-11-16
DK1590D0 (en) 1990-01-04

Similar Documents

Publication Publication Date Title
HUT64764A (en) Carbapenem-derivatives with antibiotic effect and medical preparatives containing them and process for the production thereof
AU2365388A (en) Abrasive products
AU2588888A (en) Disposable products
HU912263D0 (en) Process for the production of 5-alkyl-kynolone -carbonic acids, and antibacterial substances and fodder additives containing them
AU2094588A (en) Fitted bed sheets
AU7687987A (en) Starch-free yoghurt product
EP0370103A4 (en) Protease-deficient gram-positive bacteria and their use as host organisms for the production of recombinant products
AU1853688A (en) Control of bacteria
GB8700354D0 (en) Culture of micro-organisms
AU1092288A (en) Device permitting the prolonged storage of nutrient products
GB2256430B (en) Transparent ceramics and production process for the same
AU3300789A (en) Floor treatment product
AU6003886A (en) Fitted bedding article
EP0292808A3 (en) Substituted vinyl cephalosporins, process for their preparation and their use as medicines
AU1171692A (en) Method and container for the conservation of animal organism
AU7897087A (en) Genetic transformation of gram-positive lactic acid-producing bacteria
AU1570588A (en) New intermediate products and process
ZA882517B (en) Organic compounds and their use as pharmaceuticals
AU5381890A (en) Interferon for the use in the prevention or treatment of swine pneumonia or related bacterial diseases
EP0545490A3 (en) Antibiotic ab-041 and the process for its production
EP0211490A3 (en) Antibiotics of the vancomycin-class
GB2183665B (en) Bacterial process for the production of dispersants
AP9000186A0 (en) Bacterial strains and their application for the protection of cultures
GB8627774D0 (en) Differentiation of lactobacillus from streptococcos organisms
EP0413615A3 (en) Process for the protection and conservation of micro-organisms, particularly of bacteria

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 19900104

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AT BE CH DE FR GB IT LI LU NL SE

A4 Supplementary search report drawn up and despatched

Effective date: 19911010

AK Designated contracting states

Kind code of ref document: A4

Designated state(s): AT BE CH DE FR GB IT LI LU NL SE

DA4 Supplementary search report drawn up and despatched (deleted)
RA4 Supplementary search report drawn up and despatched (corrected)

Effective date: 19920421

RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: APPLIED MICROBIOLOGY, INC.

17Q First examination report despatched

Effective date: 19931021

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 19940503